FastQCFastQC Report
Wed 25 May 2016
SRR2049442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673692
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99290.37135915430797567No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32850.045.377921
TATAACG2250.043.8661842
GTCTCGC10750.035.8695561
GTATCAA55400.033.018741
ATAACGC2850.032.9820943
TCGCTCT11700.030.9307044
TCTCGCT12950.030.8490832
TACCTGG23900.029.8908862
ATCAACG59150.028.922763
TCAACGC59350.028.5084
TCTGTCG10250.028.4278938
GTGGTAT16850.028.1865631
CTCGCTC12650.027.8653093
CGCTCTG11900.027.6472745
TGGTATC16650.027.381082
GTACCTG27300.027.0431751
CAACGCA64950.026.1957385
TATCAAC66550.025.9891992
AACGCAG67900.025.05816
TAACGCA4050.023.2091884
Produced by FastQC (version 0.11.5)