##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049442_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2673692
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.58794318866945	31.0	31.0	34.0	27.0	34.0
2	30.777494939581672	31.0	31.0	34.0	27.0	34.0
3	30.854559911912066	31.0	31.0	34.0	27.0	34.0
4	34.376843705258494	37.0	35.0	37.0	31.0	37.0
5	34.392499584843726	37.0	35.0	37.0	31.0	37.0
6	34.444293508751194	37.0	35.0	37.0	31.0	37.0
7	34.338812398735534	37.0	35.0	37.0	30.0	37.0
8	34.28588184428124	37.0	35.0	37.0	30.0	37.0
9	35.765645407174794	39.0	37.0	39.0	30.0	39.0
10-11	35.68781015165547	39.0	37.0	39.0	30.0	39.0
12-13	35.58043390936578	39.0	37.0	39.0	30.0	39.0
14-15	36.777900932493345	40.0	37.0	41.0	30.0	41.0
16-17	36.76027754879769	40.0	37.0	41.0	30.0	41.0
18-19	36.79267507252144	40.0	37.0	41.0	30.0	41.0
20-21	36.84846553006105	40.0	37.0	41.0	30.0	41.0
22-23	36.8103164089207	40.0	37.0	41.0	30.0	41.0
24-25	36.82981753320876	40.0	37.0	41.0	30.0	41.0
26-27	36.840668072463096	40.0	37.0	41.0	30.0	41.0
28-29	36.81302277898875	40.0	37.0	41.0	30.0	41.0
30-31	36.74866775978684	40.0	37.0	41.0	30.0	41.0
32-33	36.68011031188334	40.0	37.0	41.0	30.0	41.0
34-35	36.60308199298947	40.0	37.0	41.0	30.0	41.0
36-37	36.46697394464284	40.0	37.0	41.0	30.0	41.0
38-39	36.32756203781139	40.0	36.0	41.0	29.5	41.0
40-41	36.193470676502756	40.0	36.0	41.0	29.0	41.0
42-43	36.09070248181167	40.0	36.0	41.0	28.5	41.0
44-45	35.940477063177056	40.0	36.0	41.0	28.0	41.0
46-47	35.80015443065245	40.0	35.0	41.0	28.0	41.0
48-49	35.67708883446561	40.0	35.0	41.0	27.0	41.0
50-51	35.5645362293039	39.0	35.0	41.0	27.0	41.0
52-53	35.422222903760044	39.0	35.0	41.0	27.0	41.0
54-55	35.251372072774274	39.0	35.0	41.0	26.5	41.0
56-57	35.08665209006871	39.0	35.0	41.0	26.0	41.0
58-59	34.894514027793775	39.0	34.5	41.0	26.0	41.0
60-61	34.661366567278506	38.0	34.0	40.0	26.0	41.0
62-63	34.34962759360465	38.0	34.0	40.0	25.5	41.0
64-65	34.06847179854673	37.0	34.0	40.0	25.0	41.0
66-67	33.82156452575689	37.0	34.0	40.0	24.5	41.0
68-69	33.499534725764974	36.0	33.0	39.5	24.0	41.0
70-71	33.144499628229426	36.0	33.0	39.0	23.0	41.0
72-73	32.78796379687713	35.0	33.0	39.0	22.0	40.5
74-75	32.50137169875961	35.0	33.0	38.0	22.0	40.0
76-77	32.188875345402536	35.0	33.0	37.0	20.5	39.5
78-79	31.847404263467894	35.0	32.0	37.0	20.0	39.0
80-81	31.524955567058583	35.0	32.0	36.0	18.5	39.0
82-83	31.244490764082027	35.0	32.0	36.0	17.5	38.0
84-85	30.96502401922136	35.0	32.0	36.0	13.0	37.0
86-87	30.68915005916912	35.0	32.0	35.0	7.5	37.0
88-89	30.460006238564503	35.0	32.0	35.0	2.0	36.5
90-91	30.24246229558229	35.0	31.0	35.0	2.0	36.0
92-93	30.012034482655444	35.0	31.0	35.0	2.0	36.0
94-95	29.78100562817258	35.0	31.0	35.0	2.0	36.0
96-97	29.557702046458605	35.0	31.0	35.0	2.0	35.0
98-99	29.358006456989063	35.0	31.0	35.0	2.0	35.0
100	29.20815000381495	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3270299078283294
1101	2	0.40880231065552763
1101	3	0.39183228359644673
1101	4	0.4140908758585482
1101	5	0.4060851279286908
1101	6	0.4114031236751572
1101	7	0.42792319562964565
1101	8	0.4367142196682039
1101	9	0.580539252009153
1101	10-11	0.528703339778879
1101	12-13	0.5832709830584051
1101	14-15	0.6515525636724178
1101	16-17	0.6457698415211652
1101	18-19	0.6439249178125763
1101	20-21	0.527579720079693
1101	22-23	0.5257728810084004
1101	24-25	0.5290366409062486
1101	26-27	0.5186556540154328
1101	28-29	0.43067555845404826
1101	30-31	0.443887251899703
1101	32-33	0.4395071946391482
1101	34-35	0.4067280313881483
1101	36-37	0.4473925848780169
1101	38-39	0.33347682960303615
1101	40-41	0.39322074495045456
1101	42-43	0.38313035316002697
1101	44-45	0.32961489677249745
1101	46-47	0.3101228085313892
1101	48-49	0.24323075476140588
1101	50-51	0.2829659482583935
1101	52-53	0.3000026116598704
1101	54-55	0.235275052026509
1101	56-57	0.264235840098884
1101	58-59	0.24077776892966085
1101	60-61	0.2633765199205982
1101	62-63	0.2594897773362419
1101	64-65	0.2642830483753187
1101	66-67	0.2539396216489749
1101	68-69	0.18446385937995302
1101	70-71	0.15692055658258397
1101	72-73	0.1701843724150791
1101	74-75	0.2125004899251408
1101	76-77	0.17411534934059603
1101	78-79	0.18798548088090783
1101	80-81	0.24521608784927196
1101	82-83	0.23485386680959408
1101	84-85	0.23249299412090352
1101	86-87	0.15323019347336952
1101	88-89	0.07798219772755743
1101	90-91	0.12491857721900956
1101	92-93	0.08294070676365806
1101	94-95	0.06520741571621969
1101	96-97	0.06985972833244602
1101	98-99	0.04014416302566204
1101	100	0.06911848117846375
1102	1	0.293254260487366
1102	2	0.33827191071285156
1102	3	0.4198104952145769
1102	4	0.34541933343462006
1102	5	0.3597975943321785
1102	6	0.4498603261192571
1102	7	0.4715950655877421
1102	8	0.5334762249278313
1102	9	0.6015139742965658
1102	10-11	0.611758080362911
1102	12-13	0.5786186229765562
1102	14-15	0.7290440352486769
1102	16-17	0.5924568616734902
1102	18-19	0.6116038052509722
1102	20-21	0.5349375124249676
1102	22-23	0.5997869101461646
1102	24-25	0.6322226830658053
1102	26-27	0.5123412399724572
1102	28-29	0.571059777873792
1102	30-31	0.6065634875243902
1102	32-33	0.5822708487300687
1102	34-35	0.5367139708718014
1102	36-37	0.5753115815527039
1102	38-39	0.6210487597465502
1102	40-41	0.6248701779281518
1102	42-43	0.6202760521672772
1102	44-45	0.5802072483885965
1102	46-47	0.5199073136903394
1102	48-49	0.4837814747623881
1102	50-51	0.5091548204749401
1102	52-53	0.4872059809432443
1102	54-55	0.4741135770325684
1102	56-57	0.4601436119752975
1102	58-59	0.5333751650572509
1102	60-61	0.5120599538567916
1102	62-63	0.46567807859197785
1102	64-65	0.47866989389127923
1102	66-67	0.4739756229112331
1102	68-69	0.5212222431629812
1102	70-71	0.4188426791463513
1102	72-73	0.44404806741138003
1102	74-75	0.5217575275831621
1102	76-77	0.5375125725948351
1102	78-79	0.50968113418703
1102	80-81	0.5065450905069113
1102	82-83	0.5100991899046683
1102	84-85	0.5082512519043689
1102	86-87	0.42602054685605495
1102	88-89	0.4183711967200452
1102	90-91	0.45410594795421133
1102	92-93	0.4051850404629107
1102	94-95	0.4611250621413845
1102	96-97	0.49137024800220885
1102	98-99	0.4133194944134111
1102	100	0.3528821189331097
1103	1	0.2558991482288775
1103	2	0.3483686065407845
1103	3	0.3139188069303529
1103	4	0.35899571511362893
1103	5	0.3588398511367288
1103	6	0.3588430647720031
1103	7	0.30360073011810584
1103	8	0.34168698671166453
1103	9	0.3273242647074923
1103	10-11	0.3887664766164036
1103	12-13	0.44460407016214987
1103	14-15	0.5457581911279235
1103	16-17	0.47050846187128315
1103	18-19	0.4811676876756579
1103	20-21	0.49440571359634333
1103	22-23	0.5053338642786969
1103	24-25	0.5080875697973184
1103	26-27	0.46205575545356226
1103	28-29	0.44963487569633287
1103	30-31	0.4828901582808811
1103	32-33	0.5188469660094057
1103	34-35	0.531634079603684
1103	36-37	0.4501221497892516
1103	38-39	0.4402140287667393
1103	40-41	0.484192349008012
1103	42-43	0.44629141633625125
1103	44-45	0.38879393677275687
1103	46-47	0.40194966310343005
1103	48-49	0.4042308901309184
1103	50-51	0.4411150808834421
1103	52-53	0.3736902220055711
1103	54-55	0.3336764998069697
1103	56-57	0.28862309567428923
1103	58-59	0.2808718356505011
1103	60-61	0.28107556167624637
1103	62-63	0.3303661289319706
1103	64-65	0.3210574412616154
1103	66-67	0.2638530973862814
1103	68-69	0.21075840100089493
1103	70-71	0.2509010557872742
1103	72-73	0.2677377290179379
1103	74-75	0.26532706965604547
1103	76-77	0.2225236894340128
1103	78-79	0.3488358119634647
1103	80-81	0.29969918333911494
1103	82-83	0.29498587259805475
1103	84-85	0.25627039029097176
1103	86-87	0.35212938157360796
1103	88-89	0.36970857390883793
1103	90-91	0.2825821133076012
1103	92-93	0.22608580423164426
1103	94-95	0.2541042362044017
1103	96-97	0.3269415702340126
1103	98-99	0.2626609973356189
1103	100	0.2694674543476694
1104	1	0.21084196317879744
1104	2	0.2671273158076879
1104	3	0.3488553680056974
1104	4	0.3970666072900002
1104	5	0.35270808217174476
1104	6	0.2503823448758098
1104	7	0.39228220776682576
1104	8	0.4252358652782462
1104	9	0.45952616263495827
1104	10-11	0.4791607707742003
1104	12-13	0.49805097295804757
1104	14-15	0.5163351182358227
1104	16-17	0.47146456099260803
1104	18-19	0.42866884412560324
1104	20-21	0.5257837324619175
1104	22-23	0.478074092168967
1104	24-25	0.48971882320529403
1104	26-27	0.5916181694376661
1104	28-29	0.5537677377972514
1104	30-31	0.5741090062758403
1104	32-33	0.6097323386332292
1104	34-35	0.6201872422753567
1104	36-37	0.5793486836180364
1104	38-39	0.6258638344745293
1104	40-41	0.6376526468107429
1104	42-43	0.6878558641217154
1104	44-45	0.7126855968663435
1104	46-47	0.6769284794672146
1104	48-49	0.743727553726167
1104	50-51	0.6665480209998691
1104	52-53	0.7127638230457052
1104	54-55	0.6928438314383314
1104	56-57	0.7053714412545489
1104	58-59	0.7575164998579211
1104	60-61	0.7233009087619777
1104	62-63	0.7208891903098191
1104	64-65	0.7006163736924904
1104	66-67	0.681011010016384
1104	68-69	0.6559997063780472
1104	70-71	0.7086921760893645
1104	72-73	0.6470596286912027
1104	74-75	0.6900016915880798
1104	76-77	0.6983998994711698
1104	78-79	0.6956367024371097
1104	80-81	0.6279298278413776
1104	82-83	0.6320943342511498
1104	84-85	0.5889887927906869
1104	86-87	0.5923411562009662
1104	88-89	0.5330653841571547
1104	90-91	0.6047715451209861
1104	92-93	0.5676946938645386
1104	94-95	0.4534750226276927
1104	96-97	0.6136822219405751
1104	98-99	0.5644787126844015
1104	100	0.48273139685566946
1105	1	0.28311910493666304
1105	2	0.3634378686526354
1105	3	0.3685116981120977
1105	4	0.30535581405477075
1105	5	0.2979315666325064
1105	6	0.3789754042929303
1105	7	0.4426900335163211
1105	8	0.3950036525919245
1105	9	0.5840935308606632
1105	10-11	0.6266845303273314
1105	12-13	0.5410650152346648
1105	14-15	0.65277033505172
1105	16-17	0.5860211690762824
1105	18-19	0.5117029032068032
1105	20-21	0.5855477014369797
1105	22-23	0.4951154220437388
1105	24-25	0.5783448482135398
1105	26-27	0.5845816010436309
1105	28-29	0.5158420166194659
1105	30-31	0.6487932409062935
1105	32-33	0.5684880748097427
1105	34-35	0.5842016199222186
1105	36-37	0.5777573046582631
1105	38-39	0.58711778769635
1105	40-41	0.659169220730476
1105	42-43	0.6351882421075601
1105	44-45	0.6470222045672074
1105	46-47	0.6256743917089551
1105	48-49	0.6293016679145964
1105	50-51	0.6179280737104165
1105	52-53	0.6215885056853097
1105	54-55	0.535007159520589
1105	56-57	0.5847880509859493
1105	58-59	0.5612858644381049
1105	60-61	0.6652067174356162
1105	62-63	0.6617241165824552
1105	64-65	0.6541433230077516
1105	66-67	0.6806646133324747
1105	68-69	0.646220050730328
1105	70-71	0.686291753173343
1105	72-73	0.7115142387282845
1105	74-75	0.6668438770037142
1105	76-77	0.6061225968243491
1105	78-79	0.5548540165292302
1105	80-81	0.565154010782102
1105	82-83	0.5702213677279815
1105	84-85	0.573066820048048
1105	86-87	0.5378528884728766
1105	88-89	0.5720791862099297
1105	90-91	0.5290457279091427
1105	92-93	0.40450936588270636
1105	94-95	0.4347633256403931
1105	96-97	0.4653878944688685
1105	98-99	0.48739383460909735
1105	100	0.4520312192398137
1106	1	0.22304735246448004
1106	2	0.23003513007970966
1106	3	0.20792112629790793
1106	4	0.23557380316442078
1106	5	0.1773562518301688
1106	6	0.17929916051259198
1106	7	0.24574710162046642
1106	8	0.192315666578196
1106	9	0.19282503375727345
1106	10-11	0.24363812594366863
1106	12-13	0.27808067087136124
1106	14-15	0.35375627391440645
1106	16-17	0.33333480184364106
1106	18-19	0.3562903062569873
1106	20-21	0.3601846391887733
1106	22-23	0.38746799661390696
1106	24-25	0.33273933302633907
1106	26-27	0.31858430036110974
1106	28-29	0.26817012736028545
1106	30-31	0.2905509134228339
1106	32-33	0.31557244770406356
1106	34-35	0.26952868452574563
1106	36-37	0.20825170064044585
1106	38-39	0.22103438809416076
1106	40-41	0.3269410090181921
1106	42-43	0.2135815613979659
1106	44-45	0.13383379261170347
1106	46-47	0.17270322017684236
1106	48-49	0.10475285255883904
1106	50-51	0.2207098478540317
1106	52-53	0.14489379363611476
1106	54-55	0.10519771029062497
1106	56-57	0.10058442046463512
1106	58-59	0.16300258179285976
1106	60-61	0.1483723045776344
1106	62-63	0.21160958781975125
1106	64-65	0.22772866008856596
1106	66-67	0.2883612092381682
1106	68-69	0.2145774330803576
1106	70-71	0.23648924446733588
1106	72-73	0.25906593349092333
1106	74-75	0.24081134759754974
1106	76-77	0.20617682599114318
1106	78-79	0.2254204280355303
1106	80-81	0.23850917742549527
1106	82-83	0.2739924194702965
1106	84-85	0.2024826019496686
1106	86-87	0.2375321674677764
1106	88-89	0.203808408648662
1106	90-91	0.2180763613179515
1106	92-93	0.1351046321620828
1106	94-95	0.28229195277237906
1106	96-97	0.3979633347267395
1106	98-99	0.3858617594733751
1106	100	0.28327167096067996
1107	1	0.05017210948455286
1107	2	0.06427402716551356
1107	3	0.09209952235714525
1107	4	0.016726757566161155
1107	5	0.051079680169628716
1107	6	0.049668099675884037
1107	7	0.08183811077758918
1107	8	0.0529376951971301
1107	9	0.03802437488158006
1107	10-11	0.07582252526605515
1107	12-13	0.03718744152173059
1107	14-15	0.07161246882919414
1107	16-17	0.09257428442325022
1107	18-19	0.16189855625503924
1107	20-21	0.16121608410941946
1107	22-23	0.10912521731384572
1107	24-25	0.16430837835917345
1107	26-27	0.18632749497145085
1107	28-29	0.17603955443507857
1107	30-31	0.18028876832966034
1107	32-33	0.15532045117019067
1107	34-35	0.13655152659074332
1107	36-37	0.13261487854468612
1107	38-39	0.1530909546023551
1107	40-41	0.29755156195854227
1107	42-43	0.2047029381671308
1107	44-45	0.21267716911544454
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2216	86-87	-1.1716123320467844
2216	88-89	-1.1236373363657748
2216	90-91	-1.225681006715277
2216	92-93	-1.189564206108063
2216	94-95	-1.2036103573124173
2216	96-97	-1.2259977043355974
2216	98-99	-1.278076960331017
2216	100	-1.25449888893964
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	59632.0
3	10827.0
4	6667.0
5	5558.0
6	4465.0
7	4644.0
8	5193.0
9	5659.0
10	6447.0
11	7246.0
12	8304.0
13	9080.0
14	9951.0
15	10113.0
16	10021.0
17	10591.0
18	9621.0
19	8952.0
20	9242.0
21	9821.0
22	11396.0
23	14554.0
24	17452.0
25	20628.0
26	24497.0
27	26021.0
28	31056.0
29	39656.0
30	47254.0
31	53221.0
32	67732.0
33	90548.0
34	124672.0
35	181006.0
36	293484.0
37	540325.0
38	647467.0
39	230094.0
40	595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.21124140329258	19.73032550419855	12.243717707953945	22.81471538455492
2	18.096010809015294	21.237006812492613	37.597836999929676	23.06914537856242
3	18.280032470568873	25.74291389687976	30.026522619043167	25.950531013508204
4	13.363292876478978	18.837704885532673	37.541160394900395	30.257841843087952
5	12.263450127027731	36.215209513464345	33.11600968343313	18.4053306760748
6	28.470223434119006	38.18604485888322	18.736335770218385	14.607395936779385
7	25.663526460442792	32.5022379720924	22.659836845942603	19.174398721522206
8	23.991842801874867	34.25035730757103	22.31862222350229	19.43917766705181
9	23.706295171101267	18.636658455042667	22.700626831437777	34.95641954241829
10-11	22.596248889743407	27.274541463326134	28.549337661455947	21.579871985474504
12-13	24.223670561671494	25.324782203554996	28.041397503341436	22.41014973143208
14-15	22.198600819997434	27.995289185437542	25.772034578890057	24.03407541567496
16-17	20.800251518735134	30.72914435937254	25.63605830042686	22.83454582146546
18-19	20.372622251480745	28.954940835650845	28.30395532423036	22.36848158863805
20-21	22.696313237098686	29.28924876040895	26.804175192917235	21.210262809575138
22-23	21.86523803164678	30.01358609759172	26.82661378861723	21.29456208214427
24-25	21.49654143461027	29.178235586443	27.59956316008305	21.725659818863683
26-27	21.277424586478176	28.558580528800746	27.89571877785379	22.268276106867287
28-29	21.61170606023937	28.85177945783706	27.58550070145166	21.951013780471907
30-31	22.219657875404163	30.214460023370883	26.46176496551972	21.104117135705234
32-33	20.884461696860992	29.264853167949532	27.820724906976285	22.029960228213188
34-35	20.84733666458323	28.00196822782069	28.176348275331957	22.974346832264125
36-37	22.518226148437652	29.522105628153845	26.65527803187334	21.304390191535166
38-39	22.072526532797195	29.323328099907194	27.190978156898787	21.413167210396825
40-41	21.16135364763633	28.863661740334102	27.72694380786386	22.248040804165704
42-43	21.029033319867736	29.0510682705687	27.508640423717402	22.411257985846163
44-45	21.871657143861846	29.41011980459717	27.134634884122427	21.583588167418558
46-47	21.353210704586896	30.507290819715244	26.553887573668995	21.58561090202887
48-49	20.936883763844886	29.22036955605653	27.769860818420533	22.072885861678053
50-51	21.127445974692368	28.528575537803036	27.878977625314068	22.465000862190532
52-53	21.146840920792837	28.83046337575538	27.4796061290182	22.543089574433584
54-55	21.572350440370567	30.088519243569966	26.92231369840391	21.416816617655556
56-57	20.628500875645607	29.58024937055081	27.053418403352875	22.737831350450712
58-59	20.326719867625958	28.522749544066585	28.0017923157827	23.148738272524756
60-61	21.732163506234617	29.59919027507195	26.72043637171566	21.94820984697777
62-63	21.380941675435484	29.388257729494534	26.885301644148296	22.345498950921687
64-65	21.165439678692383	28.680879750190513	27.733170424944735	22.420510146172372
66-67	21.02936687723876	28.946898874521576	26.918228501147624	23.10550574709204
68-69	22.145152430419763	29.435018326363572	26.74337985014595	21.676449393070712
70-71	22.32497559869705	28.686897519765147	27.15398316431682	21.83414371722098
72-73	21.391285252924735	28.884943476733604	28.066638310575513	21.657132959766148
74-75	21.228099282070005	28.73352869428692	27.810748393808627	22.22762362983445
76-77	21.521470506329564	29.169725296541376	26.951502489389632	22.35730170773943
78-79	21.87562128479873	29.44510745994367	27.077796125258867	21.60147512999873
80-81	21.26916577914992	28.75451380355074	27.60728852318336	22.369031894115977
82-83	21.006206944663425	28.91483859456384	27.679079246787726	22.39987521398501
84-85	21.36163686691105	28.878675095384153	27.3988366873644	22.36085135034039
86-87	21.83396916831587	29.05115913867599	27.205002587667888	21.909869105340245
88-89	21.061577764814167	28.918362716864905	27.54550611614246	22.474553402178472
90-91	20.86970541234491	28.49609620483478	27.5381588870069	23.096039495813407
92-93	21.43862653944923	29.174385225551486	26.90096299561564	22.486025239383643
94-95	21.37379837343571	28.70023923583686	27.374744393967987	22.55121799675944
96-97	21.177787736212533	28.62769859658389	27.599787381599157	22.594726285604416
98-99	21.135811529467784	28.57890957948693	27.61434580548294	22.670933085562346
100	21.769121980978543	28.9823067603713	27.028693023807303	22.21987823484285
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	8067.0
1	5499.0
2	3688.5
3	4345.5
4	3768.0
5	3025.0
6	2685.0
7	2550.5
8	2513.0
9	2432.0
10	2101.5
11	1767.0
12	1636.0
13	1616.0
14	1673.5
15	1784.5
16	1879.5
17	2076.5
18	2501.5
19	3017.5
20	3697.5
21	4767.5
22	6433.0
23	8738.0
24	11655.5
25	15232.5
26	20093.5
27	26172.5
28	33644.0
29	41557.0
30	48223.0
31	53857.0
32	58985.5
33	64105.0
34	68584.5
35	72369.5
36	75530.0
37	77504.5
38	79426.0
39	80603.5
40	80957.5
41	80515.5
42	78586.5
43	77871.0
44	77257.0
45	76566.5
46	78029.0
47	80188.0
48	83303.0
49	86958.0
50	88435.0
51	86305.0
52	81512.0
53	77541.5
54	75496.5
55	73454.5
56	70281.0
57	66420.0
58	62150.0
59	56897.5
60	50112.5
61	42114.5
62	33906.0
63	26480.5
64	20467.0
65	15482.0
66	11447.0
67	8266.0
68	5807.5
69	4100.5
70	2910.5
71	2114.5
72	1557.5
73	1138.0
74	835.5
75	615.5
76	432.5
77	352.5
78	270.5
79	197.0
80	144.0
81	83.0
82	70.5
83	60.0
84	39.5
85	36.5
86	30.5
87	18.0
88	11.5
89	15.0
90	11.5
91	6.5
92	9.5
93	9.5
94	7.0
95	6.5
96	6.0
97	5.0
98	5.0
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08134818819819187
2	0.01376373942847568
3	0.018775535850801064
4	0.011557052944018981
5	0.009724381117944774
6	0.007368088770135079
7	0.019186951975021804
8	0.008415329813606055
9	0.009200760596209287
10-11	0.012099374198673594
12-13	0.01641924350299137
14-15	0.010060994310489017
16-17	0.012435987391217837
18-19	0.0077421034285175706
20-21	0.01236118445954134
22-23	0.013875943825990427
24-25	0.015016688534057028
26-27	0.004974394956487135
28-29	0.011500950745261609
30-31	0.010472410434709757
32-33	0.011762761006129352
34-35	0.008265723950253058
36-37	0.009967490645893393
38-39	0.018364119726580322
40-41	0.012622994720409082
42-43	0.007929110757708815
44-45	0.0204773025464414
46-47	0.01875683511788194
48-49	0.01711117062099898
50-51	0.010060994310489017
52-53	0.010416308235952383
54-55	0.01191236686948235
56-57	0.019504864434646923
58-59	0.0020944820869419514
60-61	0.0071623807080247095
62-63	0.014698776074431909
64-65	0.023001901490523217
66-67	0.012716498385004707
68-69	0.013371024037174065
70-71	0.02539559530417116
72-73	0.02038379888184578
74-75	0.019467462968808674
76-77	0.009182059863290161
78-79	0.011239140484393865
80-81	0.018812937316639314
82-83	0.011800162471967602
84-85	0.010921228024768746
86-87	0.016288338372557498
88-89	0.01486708267070403
90-91	0.014810980471946657
92-93	0.007910410024789693
94-95	0.014530469478159789
96-97	0.013389724770093191
98-99	0.009556074521672652
100	0.00691927118007609
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2673692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.0531447621111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.61067853901751	62.656868262703114
2	10.471011378037232	15.508226427670072
3	2.403309151086599	5.339178014205668
4	0.8885329706036668	2.631946427920878
5	0.46640065499698224	1.726921761081748
6	0.2685798838103788	1.1933511009600575
7	0.1770429214128717	0.9177409561946113
8	0.12375078477642948	0.7331307823579031
9	0.09164921066356874	0.6108221038142225
>10	0.4652366116967814	5.946565125908799
>50	0.025981105699088472	1.3226694328517024
>100	0.0075235421796657044	0.8560371065086704
>500	1.5162300964308815E-4	0.09446625854097467
>1k	1.0108200642870734E-4	0.09046154789758386
>5k	5.054100321435367E-5	0.3716146913839926
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9929	0.37135915430797567	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.740146583824913E-5	0.0
8	0.0	0.0	0.0	3.740146583824913E-5	0.0
9	0.0	0.0	0.0	3.740146583824913E-5	0.0
10-11	0.0	0.0	0.0	3.740146583824913E-5	0.0
12-13	0.0	0.0	0.0	3.740146583824913E-5	0.0
14-15	0.0	0.0	0.0	3.740146583824913E-5	0.0
16-17	0.0	0.0	0.0	9.350366459562283E-5	0.0
18-19	0.0	0.0	0.0	1.122043975147474E-4	0.0
20-21	0.0	0.0	0.0	2.057080621103702E-4	0.0
22-23	0.0	0.0	0.0	6.171241863311107E-4	0.0
24-25	0.0	0.0	0.0	0.0013090513043387197	0.0
26-27	0.0	0.0	0.0	0.003010817999979055	0.0
28-29	0.0	0.0	0.0	0.006694862385046595	0.0
30-31	0.0	0.0	0.0	0.015035389266976151	0.0
32-33	0.0	0.0	0.0	0.03513867715503506	0.0
34-35	0.0	0.0	0.0	0.06808936855853254	0.0
36-37	0.0	0.0	0.0	0.11446718619796148	0.0
38-39	0.0	0.0	0.0	0.1768341304832419	0.0
40-41	0.0	0.0	0.0	0.2602206985696183	0.0
42-43	0.0	0.0	0.0	0.34962890265595287	0.0
44-45	0.0	0.0	0.0	0.44801345854346725	0.0
46-47	0.0	0.0	0.0	0.5604796663190824	0.0
48-49	1.8700732919124565E-5	0.0	0.0	0.6890098036722255	0.0
50-51	3.740146583824913E-5	0.0	0.0	0.8213922920067083	0.0
52-53	3.740146583824913E-5	0.0	0.0	0.9581694525771853	0.0
54-55	3.740146583824913E-5	0.0	0.0	1.1167142662655234	0.0
56-57	3.740146583824913E-5	0.0	0.0	1.2980365726493552	0.0
58-59	3.740146583824913E-5	0.0	0.0	1.4924680928094933	0.0
60-61	3.740146583824913E-5	0.0	0.0	1.689686022174581	0.0
62-63	3.740146583824913E-5	0.0	0.0	1.889054535825368	0.0
64-65	3.740146583824913E-5	0.0	0.0	2.124608967674661	0.0
66-67	3.740146583824913E-5	0.0	0.0	2.4018286324677636	0.0
68-69	3.740146583824913E-5	0.0	0.0	2.6884360651862664	0.0
70-71	3.740146583824913E-5	0.0	0.0	2.990546405494724	0.0
72-73	3.740146583824913E-5	0.0	0.0	3.3477304042500036	0.0
74-75	3.740146583824913E-5	0.0	0.0	3.701361263750649	0.0
76-77	5.610219875737369E-5	0.0	0.0	4.056095466493523	0.0
78-79	7.480293167649826E-5	0.0	0.0	4.454103165211251	0.0
80-81	7.480293167649826E-5	0.0	0.0	4.889680636363501	0.0
82-83	7.480293167649826E-5	0.0	0.0	5.345230490273375	0.0
84-85	7.480293167649826E-5	0.0	0.0	5.795936854357196	0.0
86-87	7.480293167649826E-5	0.0	0.0	6.283857676950076	0.0
88	7.480293167649826E-5	0.0	0.0	6.673842761245499	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3285	0.0	45.37792	1
TATAACG	225	0.0	43.866184	2
GTCTCGC	1075	0.0	35.869556	1
GTATCAA	5540	0.0	33.01874	1
ATAACGC	285	0.0	32.982094	3
TCGCTCT	1170	0.0	30.930704	4
TCTCGCT	1295	0.0	30.849083	2
TACCTGG	2390	0.0	29.890886	2
ATCAACG	5915	0.0	28.92276	3
TCAACGC	5935	0.0	28.508	4
TCTGTCG	1025	0.0	28.427893	8
GTGGTAT	1685	0.0	28.186563	1
CTCGCTC	1265	0.0	27.865309	3
CGCTCTG	1190	0.0	27.647274	5
TGGTATC	1665	0.0	27.38108	2
GTACCTG	2730	0.0	27.043175	1
CAACGCA	6495	0.0	26.195738	5
TATCAAC	6655	0.0	25.989199	2
AACGCAG	6790	0.0	25.0581	6
TAACGCA	405	0.0	23.209188	4
>>END_MODULE