FastQCFastQC Report
Wed 25 May 2016
SRR2049442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2673692
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167800.6275965967658204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165190.6178348141820375No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127140.4755222366674995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103740.3880028066059965No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66840.24999139766285722No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42230.15794639023492607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41030.1534582143343362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34960.13075552457051898No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72850.062.9893721
TATAACG4050.044.0996932
ATAACGC4500.040.7341963
GTATCAA129450.040.1019671
GTGGTAT24700.037.346621
TGGTATC26350.034.2474022
TACCTGG22550.032.51512
TAACGCA6050.031.8519124
ATCAACG166650.030.9955523
TATCAAC167200.030.8373722
GTACCTG24250.030.6646181
TCAACGC169850.030.4115924
TCTGTCG11800.030.2706938
CAACGCA175100.029.5534485
AACGCAG183450.028.4378036
CTGTCGC14250.026.7153559
GCGGTAT1458.5549254E-725.9665471
CGCAGAG201100.025.8250378
ACGCAGA202900.025.6422637
GTCTCGC11900.024.1254311
Produced by FastQC (version 0.11.5)