##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049442_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2673692
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731016886013798	31.0	31.0	34.0	30.0	34.0
2	31.850818643284267	31.0	31.0	34.0	30.0	34.0
3	31.98220213846621	33.0	31.0	34.0	30.0	34.0
4	35.756089332653126	37.0	35.0	37.0	33.0	37.0
5	35.80086150536412	37.0	35.0	37.0	35.0	37.0
6	35.910083884007584	37.0	35.0	37.0	35.0	37.0
7	35.89641664036097	37.0	35.0	37.0	35.0	37.0
8	35.91361907055861	37.0	35.0	37.0	35.0	37.0
9	37.61991957188786	39.0	37.0	39.0	35.0	39.0
10-11	37.595894366292	39.0	37.0	39.0	35.0	39.0
12-13	37.57164606095242	39.0	37.0	39.0	35.0	39.0
14-15	38.82593058587152	40.0	38.0	41.0	35.0	41.0
16-17	38.824230315234516	40.0	38.0	41.0	35.0	41.0
18-19	38.92658054854486	40.0	38.0	41.0	35.0	41.0
20-21	38.93330682816121	40.0	38.0	41.0	35.0	41.0
22-23	38.846555998222684	40.0	38.0	41.0	35.0	41.0
24-25	38.794335323590005	40.0	38.0	41.0	35.0	41.0
26-27	38.65913182969467	40.0	38.0	41.0	35.0	41.0
28-29	38.537509182059864	40.0	38.0	41.0	34.0	41.0
30-31	38.34396388963276	40.0	38.0	41.0	34.0	41.0
32-33	38.08566974056848	40.0	37.0	41.0	34.0	41.0
34-35	37.88080620355673	40.0	37.0	41.0	33.0	41.0
36-37	37.66553196852891	40.0	37.0	41.0	33.0	41.0
38-39	37.49455472058861	40.0	37.0	41.0	33.0	41.0
40-41	37.35858954584148	40.0	37.0	41.0	33.0	41.0
42-43	37.16160257052795	40.0	37.0	41.0	32.5	41.0
44-45	36.908058407625106	40.0	36.5	41.0	31.5	41.0
46-47	36.537021653952664	40.0	36.0	41.0	30.5	41.0
48-49	36.17819872296435	40.0	36.0	41.0	29.5	41.0
50-51	35.9786792943989	40.0	35.5	41.0	29.0	41.0
52-53	35.832511560793094	39.5	35.0	41.0	28.0	41.0
54-55	35.66182772735229	39.0	35.0	41.0	28.0	41.0
56-57	35.502472423899235	39.0	35.0	41.0	28.0	41.0
58-59	35.31372386946589	39.0	35.0	41.0	27.0	41.0
60-61	35.08914489776683	39.0	35.0	41.0	27.0	41.0
62-63	34.81140086442268	38.0	35.0	40.0	26.0	41.0
64-65	34.5452821416977	37.5	34.5	40.0	26.0	41.0
66-67	34.267301357074786	37.0	34.0	40.0	26.0	41.0
68-69	33.941320092217055	36.5	34.0	39.5	26.0	41.0
70-71	33.619398195454075	36.0	34.0	39.0	25.5	41.0
72-73	33.27641029707236	35.5	34.0	39.0	25.0	41.0
74-75	32.96757984838942	35.0	34.0	38.5	24.0	40.0
76-77	32.67325294012923	35.0	33.0	37.0	24.0	39.5
78-79	32.385210974188496	35.0	33.0	37.0	24.0	39.0
80-81	32.102123206412706	35.0	33.0	36.5	23.0	39.0
82-83	31.864384902973118	35.0	33.0	36.0	23.0	38.0
84-85	31.600604332885016	35.0	33.0	36.0	21.5	37.0
86-87	31.371137363615553	35.0	33.0	35.0	20.0	37.0
88-89	31.168676122754604	35.0	33.0	35.0	20.0	36.5
90-91	30.986847774537978	35.0	33.0	35.0	19.0	36.0
92-93	30.8162140590614	35.0	33.0	35.0	17.5	36.0
94-95	30.641099460970075	35.0	33.0	35.0	12.5	36.0
96-97	30.481018008057774	35.0	32.0	35.0	3.5	35.5
98-99	30.340090032808565	35.0	32.0	35.0	2.0	35.0
100	30.222475887274975	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2796490725789944
1101	2	0.34745377930464727
1101	3	0.4747551677171131
1101	4	0.32902978587587484
1101	5	0.26925988347724683
1101	6	0.24207741871433797
1101	7	0.27876493609625186
1101	8	0.24940968296906618
1101	9	0.2910001558625339
1101	10-11	0.24778835357383144
1101	12-13	0.29529164046289225
1101	14-15	0.3819520968874883
1101	16-17	0.3246015170352621
1101	18-19	0.3314654157178225
1101	20-21	0.4286873516708667
1101	22-23	0.37978179562056624
1101	24-25	0.35705909725594154
1101	26-27	0.34667813964026095
1101	28-29	0.3502710423200739
1101	30-31	0.3953229131771536
1101	32-33	0.3570982818470867
1101	34-35	0.28772940680236303
1101	36-37	0.32293033044730635
1101	38-39	0.19028992718123305
1101	40-41	0.16489209533049376
1101	42-43	0.13036097911462718
1101	44-45	0.16818077457711667
1101	46-47	0.2761198799679505
1101	48-49	0.32191556745500094
1101	50-51	0.2512994887616884
1101	52-53	0.298854656236216
1101	54-55	0.24576060023845514
1101	56-57	0.25375775493423447
1101	58-59	0.17374860815308324
1101	60-61	0.17970939092786864
1101	62-63	0.19857562914544502
1101	64-65	0.17519737920761713
1101	66-67	0.20963487212754472
1101	68-69	0.1942928059531539
1101	70-71	0.2220283911449954
1101	72-73	0.23571547711044616
1101	74-75	0.251075657307851
1101	76-77	0.2411673923726596
1101	78-79	0.18250882559625126
1101	80-81	0.1884862194620922
1101	82-83	0.13936815359311083
1101	84-85	0.1548136255603616
1101	86-87	0.14704685031995623
1101	88-89	0.22128995665381268
1101	90-91	0.21306281279217032
1101	92-93	0.19681961740317533
1101	94-95	0.13451488352410834
1101	96-97	0.11158975878578659
1101	98-99	0.2510182467103448
1101	100	0.22698012535548173
1102	1	0.2856971325981341
1102	2	0.2625701040768611
1102	3	0.34563828557070053
1102	4	0.25690975341682787
1102	5	0.19991176058771742
1102	6	0.19639853256990136
1102	7	0.1915694357202753
1102	8	0.18324656706936082
1102	9	0.24856951910860658
1102	10-11	0.25016067145310217
1102	12-13	0.20257225605244855
1102	14-15	0.3367783816584762
1102	16-17	0.35506207375277654
1102	18-19	0.3205238334188394
1102	20-21	0.2947153213099867
1102	22-23	0.2100886458077369
1102	24-25	0.25609260456788263
1102	26-27	0.19974954483949148
1102	28-29	0.30291526208596053
1102	30-31	0.38871015998312686
1102	32-33	0.41635196329075086
1102	34-35	0.3987586792996396
1102	36-37	0.5047852284418397
1102	38-39	0.4779977637881032
1102	40-41	0.528649323120078
1102	42-43	0.546095494309867
1102	44-45	0.5964212318892166
1102	46-47	0.6411218589213235
1102	48-49	0.6644160296421049
1102	50-51	0.537127360092029
1102	52-53	0.577039173946595
1102	54-55	0.6004165429780386
1102	56-57	0.5205788182444024
1102	58-59	0.5136938994328659
1102	60-61	0.4838335233060178
1102	62-63	0.4213243412082974
1102	64-65	0.4488698508581166
1102	66-67	0.4616501373226569
1102	68-69	0.4131042337740922
1102	70-71	0.3511444241287336
1102	72-73	0.3931468797282278
1102	74-75	0.401293342179855
1102	76-77	0.4567704814485296
1102	78-79	0.40084075956341536
1102	80-81	0.4167498386969015
1102	82-83	0.404393844403117
1102	84-85	0.27809142257683916
1102	86-87	0.31242257406938734
1102	88-89	0.3229415618704863
1102	90-91	0.3618596867736805
1102	92-93	0.3335525182188732
1102	94-95	0.3249087187001791
1102	96-97	0.3640550397591582
1102	98-99	0.29989321939561364
1102	100	0.4216132231930949
1103	1	0.06849081648200794
1103	2	0.23315941631883774
1103	3	0.29499071996243487
1103	4	0.21732005960324585
1103	5	0.12468570496858433
1103	6	0.1706877620134435
1103	7	0.1402768905247953
1103	8	0.12255234781047619
1103	9	0.24379110144530358
1103	10-11	0.19156654539776952
1103	12-13	0.11047920231860076
1103	14-15	0.30319165131822956
1103	16-17	0.21084673585593805
1103	18-19	0.1727904007741614
1103	20-21	0.2398270244512517
1103	22-23	0.28730727260461464
1103	24-25	0.29584142640262456
1103	26-27	0.31184692081384924
1103	28-29	0.3301589557417941
1103	30-31	0.3672690322399461
1103	32-33	0.33416104353536014
1103	34-35	0.33980750688618144
1103	36-37	0.3461133081602483
1103	38-39	0.3775092956874673
1103	40-41	0.37694077944050264
1103	42-43	0.37830216667137506
1103	44-45	0.30803071951986993
1103	46-47	0.27832263378172684
1103	48-49	0.25685468716305593
1103	50-51	0.32369903765381025
1103	52-53	0.374860653361182
1103	54-55	0.3062910521489499
1103	56-57	0.33319756352529595
1103	58-59	0.2548401338124151
1103	60-61	0.15687037097838896
1103	62-63	0.28761858524415373
1103	64-65	0.23309671676384625
1103	66-67	0.23650622410678324
1103	68-69	0.24429686549066787
1103	70-71	0.31335552400238953
1103	72-73	0.25129222051926803
1103	74-75	0.2511467475881659
1103	76-77	0.31127769492159985
1103	78-79	0.32373011249518413
1103	80-81	0.24631926542867433
1103	82-83	0.28517087431140453
1103	84-85	0.277848687503333
1103	86-87	0.25721193998465
1103	88-89	0.20565028847862976
1103	90-91	0.2124381316245305
1103	92-93	0.16881534873563098
1103	94-95	0.22112498085161647
1103	96-97	0.2806178714506835
1103	98-99	0.2677952819724396
1103	100	0.2852208278342623
1104	1	0.1799026013889211
1104	2	0.2498436654274876
1104	3	0.3616837842202756
1104	4	0.23254492774211144
1104	5	0.18934762389734772
1104	6	0.1820144390955747
1104	7	0.20525071355162794
1104	8	0.1930736849221688
1104	9	0.23357801890248453
1104	10-11	0.2367495688561192
1104	12-13	0.17451903588168705
1104	14-15	0.2585620512532216
1104	16-17	0.27259344618295955
1104	18-19	0.27620954446425117
1104	20-21	0.31279636007900535
1104	22-23	0.30622369982177844
1104	24-25	0.3205722269646145
1104	26-27	0.37157626845548464
1104	28-29	0.30705497373503476
1104	30-31	0.370874019087573
1104	32-33	0.38730340015494846
1104	34-35	0.38307036401098316
1104	36-37	0.4444739703703391
1104	38-39	0.4345164276313014
1104	40-41	0.40197494173285264
1104	42-43	0.45094265703487224
1104	44-45	0.3906728149870773
1104	46-47	0.4720811205163642
1104	48-49	0.36353952777682963
1104	50-51	0.34588927345474474
1104	52-53	0.30830939958566717
1104	54-55	0.25352631843106366
1104	56-57	0.26742904905596276
1104	58-59	0.36895468078984806
1104	60-61	0.33923771805780945
1104	62-63	0.4052207871363436
1104	64-65	0.3242199835605106
1104	66-67	0.39477996664871995
1104	68-69	0.3582100566579456
1104	70-71	0.35226277858303945
1104	72-73	0.42118157316859595
1104	74-75	0.4267642961669651
1104	76-77	0.46148399669085904
1104	78-79	0.42165812890571885
1104	80-81	0.37779337516320766
1104	82-83	0.3636775665027585
1104	84-85	0.3468987991858121
1104	86-87	0.38239150916792397
1104	88-89	0.4007540545573036
1104	90-91	0.3960581559106977
1104	92-93	0.37094636080377086
1104	94-95	0.3004307074539305
1104	96-97	0.2595958582716058
1104	98-99	0.300558456528762
1104	100	0.25511867260206955
1105	1	0.1831942367446544
1105	2	0.15880333528336266
1105	3	0.280059271845559
1105	4	0.196863850141213
1105	5	0.17610570045330576
1105	6	0.14924767065277678
1105	7	0.20342087913687124
1105	8	0.12298532637504422
1105	9	0.2149724600783003
1105	10-11	0.22443109250776416
1105	12-13	0.20151793545674934
1105	14-15	0.22589136541414234
1105	16-17	0.2570670036678848
1105	18-19	0.2680701585995635
1105	20-21	0.2031519274330904
1105	22-23	0.225639036311307
1105	24-25	0.28414807819891763
1105	26-27	0.30091796773683654
1105	28-29	0.36448112917427977
1105	30-31	0.3614372926894944
1105	32-33	0.3732412204591924
1105	34-35	0.3934732216206598
1105	36-37	0.37436172120106903
1105	38-39	0.42266817004476565
1105	40-41	0.4366716147435383
1105	42-43	0.45204661875883545
1105	44-45	0.4565784075702126
1105	46-47	0.38081318798653285
1105	48-49	0.49338800644698466
1105	50-51	0.44094069689198534
1105	52-53	0.4205453579331859
1105	54-55	0.4594408389639568
1105	56-57	0.4275247544028318
1105	58-59	0.438102948556768
1105	60-61	0.4298315120162073
1105	62-63	0.3869962993387972
1105	64-65	0.4585401001439777
1105	66-67	0.37010310378352784
1105	68-69	0.4814764435442811
1105	70-71	0.5414906530929855
1105	72-73	0.5579064247693424
1105	74-75	0.5820093383600877
1105	76-77	0.5162324606868225
1105	78-79	0.5034984890639507
1105	80-81	0.5371501702664148
1105	82-83	0.4551621096516527
1105	84-85	0.48175168704538507
1105	86-87	0.5321261394881134
1105	88-89	0.48967068523200297
1105	90-91	0.4674150752867483
1105	92-93	0.4742196603670692
1105	94-95	0.459799777274462
1105	96-97	0.4976630646374822
1105	98-99	0.4972379513152667
1105	100	0.4246490238864489
1106	1	0.13088292085773645
1106	2	0.16124324309378224
1106	3	0.25272635534835786
1106	4	0.11624045754025758
1106	5	0.1223826051010306
1106	6	0.17531874728385333
1106	7	0.1236815433632259
1106	8	0.1069079972019793
1106	9	0.11543826433933191
1106	10-11	0.13559117174710167
1106	12-13	0.181732484656564
1106	14-15	0.22408865031354708
1106	16-17	0.24302782107140786
1106	18-19	0.15752471030848625
1106	20-21	0.13441281626531776
1106	22-23	0.14347618288586972
1106	24-25	0.19587078504373068
1106	26-27	0.2316196688734209
1106	28-29	0.27021960699394754
1106	30-31	0.2505006052417258
1106	32-33	0.253225902743047
1106	34-35	0.21912827150946868
1106	36-37	0.19595454109750676
1106	38-39	0.2125105210005529
1106	40-41	0.17999697557996086
1106	42-43	0.194289116214307
1106	44-45	0.24767207631499133
1106	46-47	0.284339095566267
1106	48-49	0.34781253988391825
1106	50-51	0.2616961010915162
1106	52-53	0.21037061051362116
1106	54-55	0.28756544059204714
1106	56-57	0.34714529187862553
1106	58-59	0.26102974525202427
1106	60-61	0.2672136192240302
1106	62-63	0.2925102724548978
1106	64-65	0.3841240248382789
1106	66-67	0.3752924101155912
1106	68-69	0.320091556079916
1106	70-71	0.37340239310055523
1106	72-73	0.4203699263142653
1106	74-75	0.32547361084951376
1106	76-77	0.2975790437434469
1106	78-79	0.3687819072821199
1106	80-81	0.2386771867112074
1106	82-83	0.290159853475501
1106	84-85	0.30175000755999193
1106	86-87	0.3143967430304109
1106	88-89	0.31324894014550964
1106	90-91	0.29145923563510934
1106	92-93	0.23345490770670452
1106	94-95	0.2901875360517998
1106	96-97	0.3127496997783865
1106	98-99	0.28825867373680225
1106	100	0.21682140788560744
1107	1	0.05284128053829207
1107	2	0.14907746247440556
1107	3	0.1943131996928784
1107	4	0.05576870566472536
1107	5	0.12936130093309117
1107	6	0.0681234187437596
1107	7	0.033582243613814455
1107	8	0.05424829400830333
1107	9	0.07196612994118112
1107	10-11	0.04199842562501743
1107	12-13	0.05843412481987542
1107	14-15	0.13775719836696965
1107	16-17	0.07538006195136404
1107	18-19	0.0840684623089274
1107	20-21	0.1135060034593991
1107	22-23	0.12043914845101966
1107	24-25	0.09282309462163596
1107	26-27	0.11666342477715119
1107	28-29	0.1325551808169081
1107	30-31	0.13165043700983858
1107	32-33	0.13473347122631196
1107	34-35	0.12988574436207756
1107	36-37	0.18341970421607812
1107	38-39	0.15343595250948994
1107	40-41	0.21658194634431283
1107	42-43	0.24402291169742085
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2216	64-65	-0.740810543646532
2216	66-67	-0.6577386615988061
2216	68-69	-0.7379776461002194
2216	70-71	-0.7497111173827875
2216	72-73	-0.6254044172515023
2216	74-75	-0.6917068615678517
2216	76-77	-0.5807754882047647
2216	78-79	-0.6000971844301617
2216	80-81	-0.7033198616553307
2216	82-83	-0.6249800865037969
2216	84-85	-0.7268245448458011
2216	86-87	-0.6270289593735896
2216	88-89	-0.6651298507352124
2216	90-91	-0.5846104324829327
2216	92-93	-0.595494189096577
2216	94-95	-0.6636940231553403
2216	96-97	-0.652674431125206
2216	98-99	-0.6513797719650505
2216	100	-0.7223407255068004
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	179.0
3	20.0
4	45.0
5	120.0
6	443.0
7	1084.0
8	2030.0
9	3295.0
10	4703.0
11	6524.0
12	9872.0
13	13855.0
14	13837.0
15	15455.0
16	19495.0
17	26989.0
18	27228.0
19	14388.0
20	9769.0
21	8974.0
22	9086.0
23	9584.0
24	10766.0
25	12759.0
26	15693.0
27	19511.0
28	24534.0
29	31683.0
30	40313.0
31	51509.0
32	66269.0
33	87429.0
34	121840.0
35	186626.0
36	285054.0
37	557505.0
38	705499.0
39	258912.0
40	815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.109404146523325	19.621254592757122	12.944693673258218	22.32464758746134
2	17.961044129241515	21.054668974586452	37.974344090493595	23.009942805678442
3	17.844650767552885	25.80645040640433	31.084956681622266	25.263942144420525
4	13.168046282069886	18.58209546948564	37.254403274573136	30.995454973871333
5	11.44841826486068	35.43191450363404	34.05658859923765	19.063078632267622
6	27.955975383796005	37.79209675332107	18.682448125920544	15.56947973696238
7	24.538503313021845	32.6000526612639	23.518191324954408	19.343252700759848
8	24.260498217446138	32.54604494459347	22.999807008436274	20.19364982952412
9	23.77334412490294	16.372977889749453	24.039455554342084	35.81422243100552
10-11	22.64494190056297	25.90633102092537	29.306273871485573	22.14245320702609
12-13	24.97884947106847	24.008150527435472	29.37726185364657	21.63573814784949
14-15	22.44364721142151	26.025866105744417	28.470949533454114	23.059537149379956
16-17	19.8125662940982	28.879971963861205	28.425469350994803	22.88199239104579
18-19	19.243390786971723	25.61925606988389	33.253231860663085	21.884121282481303
20-21	21.397060693602704	25.835960162950705	32.481396510892054	20.285582632554537
22-23	20.63087296517325	26.56837436772822	32.552945515040626	20.247807152057902
24-25	20.286199756740867	25.792312652317467	33.15525872089979	20.766228870041875
26-27	20.093937521599347	25.1781618825205	33.550816623605115	21.17708397227504
28-29	20.48626019750966	25.39479117265564	33.205152276328015	20.913796353506687
30-31	21.145180521915012	26.72787291879543	32.07461816843526	20.052328390854292
32-33	19.817578090520524	25.898532815298097	33.36399630174306	20.919892792438322
34-35	19.786385268011426	24.770822518076127	33.42957977209043	22.01321244182202
36-37	21.63813184166314	26.375008789344474	31.703202911928525	20.283656457063863
38-39	21.29461807867174	26.087746830973803	32.12002354796289	20.49761154239157
40-41	20.29487689681534	25.846208164590383	32.47526267049458	21.38365226809969
42-43	20.26323525671618	26.1488608261535	31.97047378680865	21.61743013032167
44-45	21.252859342063335	26.433766492176364	31.417437012191378	20.895937153568923
46-47	20.847146941382928	27.759442748080183	30.30474340350347	21.08866690703342
48-49	20.699542056452277	26.646655635727672	31.081646651895582	21.572155655924465
50-51	20.921145741543903	26.012551183906	30.88302616756156	22.18327690698854
52-53	20.87953661079885	26.463070540660627	30.397742148310275	22.259650700230242
54-55	21.357340336882483	27.754075637732395	29.766947726215285	21.12163629916984
56-57	20.369679828491837	27.194325299997157	29.894449323257877	22.54154554825313
58-59	19.995758673773942	26.094927912414747	30.96143834069145	22.947875073119867
60-61	21.35848108159055	27.08763761869355	29.729920275035422	21.82396102468048
62-63	21.0729021891826	26.84523871859586	29.977031759828733	22.104827332392812
64-65	20.872374230090827	26.19774454200409	30.794048080332363	22.13583314757272
66-67	20.764190490153688	26.5611184833556	29.84653804551908	22.82815298097163
68-69	21.917838703934482	26.99622843618487	29.580931535868753	21.50500132401189
70-71	22.16450137113774	26.288630103991036	29.872588166475424	21.674280358395805
72-73	21.318854228534924	26.56353087790217	30.60980845961315	21.507806433949757
74-75	21.107386340685462	26.362180086561953	30.394394717117752	22.13603885563483
76-77	21.419183660646027	26.918096774048767	29.417393626491013	22.245325938814194
78-79	21.770364724134268	27.257178463338334	29.430091424143097	21.5423653883843
80-81	21.05606562919832	26.52495354730993	30.065718213942688	22.353262609549056
82-83	20.760558338963836	26.755945596341824	30.02711056911998	22.45638549557436
84-85	21.12206271274493	26.71994024721704	29.69596620092958	22.46203083910845
86-87	21.516064568708508	26.890251223127382	29.458379259439894	22.13530494872421
88-89	20.661060274913794	26.774818699882875	29.728861908957132	22.835259116246203
90-91	20.3401238034487	26.440812612310367	29.718849365863107	23.500214218377824
92-93	20.91983535831633	27.09836798276536	29.05420795643466	22.92758870248365
94-95	20.7768788107504	26.63106288472169	29.581633744575843	23.010424559952067
96-97	20.465370697635276	26.643571114664272	29.74398533853217	23.14707284916828
98-99	20.380751479245678	26.56205351465781	29.73814920371326	23.31904580238325
100	20.911271754562605	26.932197126669788	29.133422997114106	23.023108121653504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3169.0
1	2367.0
2	6213.5
3	8924.0
4	4307.0
5	1687.5
6	2788.5
7	6016.0
8	9151.5
9	9993.0
10	7214.5
11	3896.5
12	3248.5
13	3351.0
14	3700.5
15	4109.5
16	4512.5
17	4933.0
18	5413.0
19	5957.5
20	6549.5
21	7503.5
22	8917.5
23	10997.0
24	13583.0
25	16891.0
26	21525.0
27	27090.5
28	33830.0
29	41185.0
30	47571.0
31	53104.5
32	57943.5
33	62693.5
34	66980.5
35	70319.0
36	72865.5
37	74839.5
38	76842.5
39	77855.5
40	78102.5
41	77965.5
42	76885.5
43	76001.5
44	75417.5
45	74826.0
46	75804.0
47	78370.5
48	82437.5
49	86382.0
50	87475.5
51	84972.5
52	79728.5
53	75434.0
54	73217.5
55	71563.5
56	68787.5
57	65018.0
58	61263.0
59	56587.0
60	49481.0
61	40950.5
62	33046.5
63	25694.5
64	19638.0
65	14836.0
66	10844.0
67	7674.0
68	5187.0
69	3506.0
70	2380.5
71	1633.5
72	1188.5
73	860.5
74	650.5
75	472.5
76	299.5
77	219.5
78	175.5
79	120.5
80	67.0
81	28.5
82	16.5
83	9.0
84	4.5
85	2.0
86	3.0
87	1.5
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12922206447115075
2	0.0
3	0.0
4	0.0
5	0.013539330633446185
6	2.244087950294948E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.4960586335299654E-4
82-83	4.30116857139865E-4
84-85	3.366131925442422E-4
86-87	4.8621905589723874E-4
88-89	6.545256521693598E-4
90-91	6.91927118007609E-4
92-93	2.618102608677439E-4
94-95	2.244087950294948E-4
96-97	6.358249192502353E-4
98-99	0.0011968469068239724
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2673692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.23908603215733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.79004633119598	58.85336272895324
2	10.983911886922828	15.429998639904163
3	2.531660644390526	5.334645894167191
4	0.9321078404486767	2.61881611186092
5	0.480480556625786	1.6874257576808716
6	0.30297461602973663	1.2768396072523507
7	0.19423483480227596	0.9550014090483252
8	0.13048305836521765	0.7332008609802824
9	0.10335238336505624	0.6533439252126023
>10	0.5079367648043244	6.295363848488397
>50	0.029564746024937003	1.4333591568239716
>100	0.011810559132750677	1.355995265045885
>500	7.97654384433066E-4	0.3902060009874669
>1k	3.722387127002068E-4	0.6266752449614571
>5k	5.31769589571724E-5	0.24965415051390982
>10k+	2.127078358286896E-4	2.106111398119065
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16780	0.6275965967658204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16519	0.6178348141820375	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12714	0.4755222366674995	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10374	0.3880028066059965	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6684	0.24999139766285722	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4223	0.15794639023492607	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4103	0.1534582143343362	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3496	0.13075552457051898	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.740146583824913E-5	0.0
8	0.0	0.0	0.0	1.122043975147474E-4	0.0
9	0.0	0.0	0.0	1.122043975147474E-4	0.0
10-11	0.0	0.0	0.0	1.122043975147474E-4	0.0
12-13	0.0	1.8700732919124565E-5	0.0	1.8700732919124565E-4	0.0
14-15	0.0	3.740146583824913E-5	0.0	1.8700732919124565E-4	0.0
16-17	0.0	3.740146583824913E-5	0.0	1.8700732919124565E-4	0.0
18-19	0.0	3.740146583824913E-5	0.0	1.8700732919124565E-4	0.0
20-21	0.0	3.740146583824913E-5	0.0	3.1791245962511764E-4	0.0
22-23	0.0	3.740146583824913E-5	0.0	7.293285838458581E-4	0.0
24-25	0.0	3.740146583824913E-5	0.0	0.0014960586335299652	0.0
26-27	0.0	3.740146583824913E-5	0.0	0.0031791245962511762	0.0
28-29	0.0	3.740146583824913E-5	0.0	0.0069379719129952135	0.0
30-31	0.0	3.740146583824913E-5	0.0	0.015428104658277767	0.0
32-33	0.0	3.740146583824913E-5	0.0	0.03581190354012354	0.0
34-35	0.0	3.740146583824913E-5	0.0	0.06894960227281227	0.0
36-37	0.0	3.740146583824913E-5	0.0	0.11521521551472645	0.0
38-39	0.0	3.740146583824913E-5	0.0	0.17799357592422763	0.0
40-41	0.0	3.740146583824913E-5	0.0	0.2622403777248838	0.0
42-43	0.0	3.740146583824913E-5	0.0	0.35245271332674066	0.0
44-45	0.0	3.740146583824913E-5	0.0	0.45106167800928454	0.0
46-47	0.0	3.740146583824913E-5	0.0	0.5635465865178189	0.0
48-49	0.0	3.740146583824913E-5	0.0	0.6922076290013959	0.0
50-51	0.0	3.740146583824913E-5	0.0	0.8254316503172392	0.0
52-53	0.0	3.740146583824913E-5	0.0	0.9627885336082092	0.0
54-55	0.0	3.740146583824913E-5	0.0	1.1221000773462313	0.0
56-57	0.0	3.740146583824913E-5	0.0	1.304282617444343	0.0
58-59	0.0	3.740146583824913E-5	0.0	1.4994995683870842	0.0
60-61	0.0	3.740146583824913E-5	0.0	1.6985501695782461	0.0
62-63	0.0	3.740146583824913E-5	0.0	1.899246435266291	0.0
64-65	0.0	3.740146583824913E-5	0.0	2.134950472978937	0.0
66-67	0.0	3.740146583824913E-5	0.0	2.412862064890047	0.0
68-69	0.0	3.740146583824913E-5	0.0	2.700741147447051	0.0
70-71	0.0	3.740146583824913E-5	0.0	3.004179239792766	0.0
72-73	0.0	3.740146583824913E-5	0.0	3.363719530895855	0.0
74-75	0.0	3.740146583824913E-5	0.0	3.7200245952039355	0.0
76-77	0.0	3.740146583824913E-5	0.0	4.0772272946921335	0.0
78-79	0.0	3.740146583824913E-5	0.0	4.477123767434693	0.0
80-81	0.0	3.740146583824913E-5	0.0	4.9154128448602155	0.0
82-83	0.0	3.740146583824913E-5	0.0	5.373375093316657	0.0
84-85	0.0	3.740146583824913E-5	0.0	5.826699560009156	0.0
86-87	0.0	3.740146583824913E-5	0.0	6.318697142378404	0.0
88	0.0	3.740146583824913E-5	0.0	6.711244227083748	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7285	0.0	62.989372	1
TATAACG	405	0.0	44.099693	2
ATAACGC	450	0.0	40.734196	3
GTATCAA	12945	0.0	40.101967	1
GTGGTAT	2470	0.0	37.34662	1
TGGTATC	2635	0.0	34.247402	2
TACCTGG	2255	0.0	32.5151	2
TAACGCA	605	0.0	31.851912	4
ATCAACG	16665	0.0	30.995552	3
TATCAAC	16720	0.0	30.837372	2
GTACCTG	2425	0.0	30.664618	1
TCAACGC	16985	0.0	30.411592	4
TCTGTCG	1180	0.0	30.270693	8
CAACGCA	17510	0.0	29.553448	5
AACGCAG	18345	0.0	28.437803	6
CTGTCGC	1425	0.0	26.715355	9
GCGGTAT	145	8.5549254E-7	25.966547	1
CGCAGAG	20110	0.0	25.825037	8
ACGCAGA	20290	0.0	25.642263	7
GTCTCGC	1190	0.0	24.125431	1
>>END_MODULE