FastQCFastQC Report
Wed 25 May 2016
SRR2049441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6492317
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64850.044.381091
GTATCAA113700.036.8117141
TCAACGC118350.033.952354
ATCAACG118900.033.6774943
CAACGCA130400.030.7084165
TATCAAC135050.030.206972
AACGCAG136550.029.2934196
ACGCAGA152100.025.9895657
CGCAGAG155950.025.0741738
GTGGTAT38350.023.1767711
GTACATG173550.022.518161
TACATGG177000.021.2687152
GCAGAGT183850.020.5278729
ACATGGG179600.019.8879153
TGGTATC41600.019.8838922
CAGAGTA174500.018.20748710-11
AGAGTAC160000.017.4928510-11
GAGTACT80250.017.2179312-13
CATGGGG131800.016.79494
GAGTACA130750.015.5381221
Produced by FastQC (version 0.11.5)