##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049441_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6492317
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.964228949387408	31.0	31.0	34.0	28.0	34.0
2	31.179877846383658	31.0	31.0	34.0	28.0	34.0
3	31.29935214192406	33.0	31.0	34.0	28.0	34.0
4	34.906888711687984	37.0	35.0	37.0	32.0	37.0
5	34.93928731452885	37.0	35.0	37.0	32.0	37.0
6	34.95381648801191	37.0	35.0	37.0	32.0	37.0
7	34.83761390579049	37.0	35.0	37.0	32.0	37.0
8	34.81654746679806	37.0	35.0	37.0	32.0	37.0
9	36.41857090465545	39.0	37.0	39.0	32.0	39.0
10-11	36.36263401802469	39.0	37.0	39.0	32.0	39.0
12-13	36.28641692942597	39.0	37.0	39.0	32.0	39.0
14-15	37.53410839304365	40.0	38.0	41.0	32.0	41.0
16-17	37.526186336865564	40.0	38.0	41.0	32.0	41.0
18-19	37.55078849353782	40.0	38.0	41.0	32.0	41.0
20-21	37.601585381613376	40.0	38.0	41.0	32.0	41.0
22-23	37.56764849898734	40.0	38.0	41.0	32.0	41.0
24-25	37.57033821669521	40.0	38.0	41.0	32.0	41.0
26-27	37.56688505813872	40.0	38.0	41.0	32.0	41.0
28-29	37.54372160201049	40.0	38.0	41.0	32.0	41.0
30-31	37.48607330788069	40.0	38.0	41.0	32.0	41.0
32-33	37.432572916572006	40.0	38.0	41.0	32.0	41.0
34-35	37.35569920569189	40.0	38.0	41.0	31.5	41.0
36-37	37.24209946926498	40.0	37.0	41.0	31.0	41.0
38-39	37.1318510017302	40.0	37.0	41.0	31.0	41.0
40-41	37.00524843441871	40.0	37.0	41.0	30.0	41.0
42-43	36.908090978921706	40.0	37.0	41.0	30.0	41.0
44-45	36.760208566525634	40.0	37.0	41.0	30.0	41.0
46-47	36.63806311367729	40.0	36.5	41.0	30.0	41.0
48-49	36.53338230403722	40.0	36.0	41.0	30.0	41.0
50-51	36.420019694047596	40.0	36.0	41.0	30.0	41.0
52-53	36.30497694428661	40.0	36.0	41.0	30.0	41.0
54-55	36.153314756503725	39.0	35.0	41.0	29.5	41.0
56-57	35.98661009621064	39.0	35.0	41.0	29.0	41.0
58-59	35.80367979567233	39.0	35.0	41.0	29.0	41.0
60-61	35.60479409739234	39.0	35.0	41.0	28.5	41.0
62-63	35.35188939480312	38.5	35.0	40.0	28.0	41.0
64-65	35.11080697384308	38.0	34.5	40.0	28.0	41.0
66-67	34.88012569318473	37.5	34.0	40.0	28.0	41.0
68-69	34.58856653179443	37.0	34.0	40.0	28.0	41.0
70-71	34.27304566305065	36.5	34.0	39.5	27.0	41.0
72-73	33.93330724608795	36.0	34.0	39.0	26.0	41.0
74-75	33.63007490238077	35.5	34.0	39.0	26.0	40.5
76-77	33.299383409651746	35.0	33.5	38.5	26.0	40.0
78-79	32.94584629801656	35.0	33.0	37.0	26.0	39.0
80-81	32.60732493499624	35.0	33.0	37.0	26.0	39.0
82-83	32.302454347192224	35.0	33.0	36.0	25.0	39.0
84-85	32.008525153654695	35.0	33.0	36.0	25.0	37.0
86-87	31.727888672102736	35.0	33.0	36.0	24.5	37.0
88-89	31.484304139800937	35.0	33.0	35.0	24.0	37.0
90-91	31.26401660608994	35.0	32.5	35.0	23.0	36.0
92-93	31.047961844746645	35.0	32.0	35.0	21.0	36.0
94-95	30.8331552510452	35.0	32.0	35.0	20.0	36.0
96-97	30.62884668139279	35.0	32.0	35.0	18.0	36.0
98-99	30.437029029851747	35.0	32.0	35.0	6.0	35.0
100	30.29718065214622	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2251135576188723
1101	2	0.26135205539252837
1101	3	0.30782592556549915
1101	4	0.358616461520036
1101	5	0.35444031831314504
1101	6	0.38238627519934454
1101	7	0.4646308894417075
1101	8	0.4875497827082782
1101	9	0.5392742673260926
1101	10-11	0.5340855279704684
1101	12-13	0.5153430083871982
1101	14-15	0.6758243089761393
1101	16-17	0.603339997856736
1101	18-19	0.596897641187681
1101	20-21	0.5668687066826834
1101	22-23	0.5853394830475978
1101	24-25	0.5588835268253831
1101	26-27	0.5472236558858938
1101	28-29	0.5776592636357023
1101	30-31	0.552637091486389
1101	32-33	0.5821702180803285
1101	34-35	0.605925860787778
1101	36-37	0.5550879083305134
1101	38-39	0.5427360128888736
1101	40-41	0.6083246552055996
1101	42-43	0.5457383864250147
1101	44-45	0.5603546467390217
1101	46-47	0.515892139428864
1101	48-49	0.5026603391906974
1101	50-51	0.5068672875792473
1101	52-53	0.5268466700241703
1101	54-55	0.44291789043443686
1101	56-57	0.4319693279547181
1101	58-59	0.4744711965034014
1101	60-61	0.4270981552267088
1101	62-63	0.3985316859255832
1101	64-65	0.4429425453780169
1101	66-67	0.4485055801135047
1101	68-69	0.402228210151506
1101	70-71	0.4162152614498993
1101	72-73	0.3804832772590032
1101	74-75	0.3519428412482952
1101	76-77	0.3581887486925339
1101	78-79	0.39551945714828207
1101	80-81	0.3790075309753931
1101	82-83	0.3629732238018093
1101	84-85	0.3629826168843451
1101	86-87	0.3381616239136811
1101	88-89	0.2908551839921536
1101	90-91	0.2795498578388589
1101	92-93	0.301212413933424
1101	94-95	0.3211172052033895
1101	96-97	0.29283260797275545
1101	98-99	0.3457885754436276
1101	100	0.3259558846898756
1102	1	0.21013710426255017
1102	2	0.2617074953779408
1102	3	0.34784945160843606
1102	4	0.32522093970848687
1102	5	0.26871729935737676
1102	6	0.25506292395366614
1102	7	0.2668956058364955
1102	8	0.3286360750801336
1102	9	0.39815583780276853
1102	10-11	0.3928958198932264
1102	12-13	0.41477679709984727
1102	14-15	0.4994954326124983
1102	16-17	0.447545744123623
1102	18-19	0.5075726217972374
1102	20-21	0.48136514996524227
1102	22-23	0.4983613769970603
1102	24-25	0.48215663132570796
1102	26-27	0.45276260209633534
1102	28-29	0.5136494612894822
1102	30-31	0.47868627420361065
1102	32-33	0.5211884023518678
1102	34-35	0.5210017255810158
1102	36-37	0.5393124424705746
1102	38-39	0.5912121505388868
1102	40-41	0.5162652998439015
1102	42-43	0.4722034465431193
1102	44-45	0.46122916094786603
1102	46-47	0.40359715539958074
1102	48-49	0.4296333491336384
1102	50-51	0.4396690961180525
1102	52-53	0.43679113558437166
1102	54-55	0.44043174185573264
1102	56-57	0.4183999050122935
1102	58-59	0.4730318744324933
1102	60-61	0.4071131860402559
1102	62-63	0.404687263729933
1102	64-65	0.4131392257750761
1102	66-67	0.44317325930644813
1102	68-69	0.46110147595561557
1102	70-71	0.42119197489054017
1102	72-73	0.4215806811539835
1102	74-75	0.44347261601012633
1102	76-77	0.41868844277156825
1102	78-79	0.39564536628460445
1102	80-81	0.4395850958445422
1102	82-83	0.4040942155419387
1102	84-85	0.41112177796048144
1102	86-87	0.3472457382724947
1102	88-89	0.336280107692172
1102	90-91	0.36295718644844044
1102	92-93	0.3256638034017101
1102	94-95	0.2761218068932685
1102	96-97	0.27303112698569265
1102	98-99	0.3010906904184054
1102	100	0.26811835962135433
1103	1	0.14195353369724373
1103	2	0.1816109230183045
1103	3	0.24209722881639806
1103	4	0.18219782498240988
1103	5	0.2751369275883562
1103	6	0.26163770830906685
1103	7	0.26607320366305487
1103	8	0.25362822512661864
1103	9	0.3249325572314774
1103	10-11	0.3079298356943454
1103	12-13	0.310154200550393
1103	14-15	0.40558777356825004
1103	16-17	0.3877444645015089
1103	18-19	0.3534673999444067
1103	20-21	0.37796983088854574
1103	22-23	0.3821333762925505
1103	24-25	0.4051022397150419
1103	26-27	0.435047906863808
1103	28-29	0.4111325799046668
1103	30-31	0.4057537059020362
1103	32-33	0.40602515121193505
1103	34-35	0.41877622889214194
1103	36-37	0.3865309040868894
1103	38-39	0.4022917124078731
1103	40-41	0.4508763575593875
1103	42-43	0.3984568938482198
1103	44-45	0.42290622083344687
1103	46-47	0.3751681203740489
1103	48-49	0.3438259853990715
1103	50-51	0.31421443637368895
1103	52-53	0.3073943148554008
1103	54-55	0.2585984270514814
1103	56-57	0.25927810959642983
1103	58-59	0.26390901309654424
1103	60-61	0.3271631776922135
1103	62-63	0.25011829192641244
1103	64-65	0.29078778813777717
1103	66-67	0.28990869475373415
1103	68-69	0.21670709488859075
1103	70-71	0.22689245074914055
1103	72-73	0.269977980650431
1103	74-75	0.24542994414097308
1103	76-77	0.17003800219790577
1103	78-79	0.23336129977757025
1103	80-81	0.25221554653148814
1103	82-83	0.17420043198787027
1103	84-85	0.1839573226297091
1103	86-87	0.17502057231770962
1103	88-89	0.16044306655084384
1103	90-91	0.20608077161386262
1103	92-93	0.12347696873009895
1103	94-95	0.2030534084949771
1103	96-97	0.21862731302401528
1103	98-99	0.22759804088376256
1103	100	0.22976750136641044
1104	1	0.07461240842608419
1104	2	0.09335702834163229
1104	3	0.1506271438886948
1104	4	0.13279659402100208
1104	5	0.15086773786193675
1104	6	0.16617460911955106
1104	7	0.1333767306711806
1104	8	0.13162464088610903
1104	9	0.2596563901167457
1104	10-11	0.23595941832261502
1104	12-13	0.22101694351068346
1104	14-15	0.2854655338855352
1104	16-17	0.2305173435574872
1104	18-19	0.22810857032871468
1104	20-21	0.23903246372599796
1104	22-23	0.2107427797231125
1104	24-25	0.2532275989084738
1104	26-27	0.2243937555360489
1104	28-29	0.19684671310606205
1104	30-31	0.20767688926299144
1104	32-33	0.20292108776057205
1104	34-35	0.22331632054506656
1104	36-37	0.2179263066018251
1104	38-39	0.2414923747671196
1104	40-41	0.2376813354167311
1104	42-43	0.20739512343284616
1104	44-45	0.16057693090384362
1104	46-47	0.08229877052102097
1104	48-49	0.09312490661997685
1104	50-51	0.1304451961902089
1104	52-53	0.07553029388816412
1104	54-55	0.10699434582682699
1104	56-57	0.09869598780906585
1104	58-59	0.1390992767686754
1104	60-61	0.15391369174587766
1104	62-63	0.14160720580658648
1104	64-65	0.13416955150108834
1104	66-67	0.1677618208131264
1104	68-69	0.09722837357306702
1104	70-71	0.1408967898177167
1104	72-73	0.14922859430608781
1104	74-75	0.1400635734015836
1104	76-77	0.10269050717025863
1104	78-79	0.10880966979982531
1104	80-81	0.13136592002108927
1104	82-83	0.14918269448243393
1104	84-85	0.11605932080085068
1104	86-87	0.10944432287326933
1104	88-89	0.09068599641934938
1104	90-91	0.1109392556643023
1104	92-93	0.08417204633973796
1104	94-95	0.09191016364559701
1104	96-97	0.09879194498687838
1104	98-99	0.07093400522076365
1104	100	0.06008453631394772
1105	1	0.12288335647340176
1105	2	0.15124434838661216
1105	3	0.21594370935861562
1105	4	0.1947972240852991
1105	5	0.21491805406034103
1105	6	0.21409776672165037
1105	7	0.2522459068245766
1105	8	0.2657845844886495
1105	9	0.2526084843125602
1105	10-11	0.2808266724188755
1105	12-13	0.2926633741712621
1105	14-15	0.40880050990967476
1105	16-17	0.33727424149778784
1105	18-19	0.3724408371451773
1105	20-21	0.36434954613059745
1105	22-23	0.35715551951532376
1105	24-25	0.3458359372712181
1105	26-27	0.382585442366711
1105	28-29	0.4017474888691339
1105	30-31	0.37349323658304456
1105	32-33	0.3956355753937686
1105	34-35	0.3655628533432207
1105	36-37	0.3447297194939978
1105	38-39	0.3777344705234853
1105	40-41	0.44123162791751014
1105	42-43	0.42659348152909615
1105	44-45	0.3858220837385602
1105	46-47	0.3244006074558641
1105	48-49	0.3542897186354139
1105	50-51	0.31352577501721157
1105	52-53	0.35598381294967396
1105	54-55	0.324254514066709
1105	56-57	0.3032547556889469
1105	58-59	0.26857147671488235
1105	60-61	0.24932036603742347
1105	62-63	0.26208300005351504
1105	64-65	0.24632867477069453
1105	66-67	0.2589945226283987
1105	68-69	0.26434695500802263
1105	70-71	0.2917136182538087
1105	72-73	0.28238523187636844
1105	74-75	0.32654181718534403
1105	76-77	0.2547645585793603
1105	78-79	0.2409099897434217
1105	80-81	0.2861725099411032
1105	82-83	0.23906673548807333
1105	84-85	0.19483104846246846
1105	86-87	0.25315595286632586
1105	88-89	0.2588311172923703
1105	90-91	0.2820309214221304
1105	92-93	0.23772614134786352
1105	94-95	0.23541094503649518
1105	96-97	0.2548667613676088
1105	98-99	0.2649812616472431
1105	100	0.20741150546687237
1106	1	0.13327667318151626
1106	2	0.13739131040976105
1106	3	0.11732808680447349
1106	4	0.13258002586918138
1106	5	0.1752554289844781
1106	6	0.16196882494936204
1106	7	0.2236607065415015
1106	8	0.17291486470229245
1106	9	0.19001679877736422
1106	10-11	0.21935378419353668
1106	12-13	0.18266953654865148
1106	14-15	0.22030965192787022
1106	16-17	0.1882893235799017
1106	18-19	0.2496352577401737
1106	20-21	0.20289251770633143
1106	22-23	0.23199933604889367
1106	24-25	0.2841792476878169
1106	26-27	0.2841435299652133
1106	28-29	0.20206289686704082
1106	30-31	0.2598090051236497
1106	32-33	0.23673711975710887
1106	34-35	0.23724088168766144
1106	36-37	0.2859391021729252
1106	38-39	0.2287933378032676
1106	40-41	0.3063337696626789
1106	42-43	0.2934479520514941
1106	44-45	0.23458516615596636
1106	46-47	0.2152855981685562
1106	48-49	0.21049334116000296
1106	50-51	0.25915769835975766
1106	52-53	0.2448554848498361
1106	54-55	0.18795259695255595
1106	56-57	0.1685393174864913
1106	58-59	0.21021602001697204
1106	60-61	0.2089320457092967
1106	62-63	0.2574153324445163
1106	64-65	0.23754069837453784
1106	66-67	0.250520990604997
1106	68-69	0.21109548311170556
1106	70-71	0.20658224150270144
1106	72-73	0.16277088518824456
1106	74-75	0.15526035074036315
1106	76-77	0.14813771934068853
1106	78-79	0.12843738810612848
1106	80-81	0.12028542589253277
1106	82-83	0.15484269020544872
1106	84-85	0.18757166769216127
1106	86-87	0.21191502160406728
1106	88-89	0.21420997754021798
1106	90-91	0.18164874044964208
1106	92-93	0.05820791073291076
1106	94-95	0.15536190166851682
1106	96-97	0.22526626851774267
1106	98-99	0.23530945066932674
1106	100	0.21453034934646453
1107	1	0.0845270787923269
1107	2	0.12255396511799788
1107	3	0.14786465619858546
1107	4	0.11710117054565927
1107	5	0.1637819824098159
1107	6	0.15877572416489016
1107	7	0.14380156705806257
1107	8	0.1700470541387844
1107	9	0.18971411075288103
1107	10-11	0.2449618460331493
1107	12-13	0.27100602374797944
1107	14-15	0.2813868302127105
1107	16-17	0.2267793717684654
1107	18-19	0.2264709753362837
1107	20-21	0.2905923412151452
1107	22-23	0.23075475975866055
1107	24-25	0.24013955342139326
1107	26-27	0.3456611030305794
1107	28-29	0.3235478624407193
1107	30-31	0.30557178727747925
1107	32-33	0.2973266791201752
1107	34-35	0.2811706183249285
1107	36-37	0.25716488183110187
1107	38-39	0.24343690854784228
1107	40-41	0.31579195567303486
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2216	88-89	-0.9726499035810967
2216	90-91	-1.006073624641239
2216	92-93	-1.0030095881767096
2216	94-95	-1.0181399126437007
2216	96-97	-1.042705714641432
2216	98-99	-1.1087798574828227
2216	100	-1.1952624077745888
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88429.0
3	17523.0
4	10688.0
5	8876.0
6	7738.0
7	7960.0
8	9081.0
9	10423.0
10	11913.0
11	13189.0
12	14989.0
13	16837.0
14	18897.0
15	20261.0
16	20145.0
17	20919.0
18	20039.0
19	18015.0
20	17927.0
21	19240.0
22	21798.0
23	26054.0
24	31820.0
25	39167.0
26	47653.0
27	54972.0
28	67813.0
29	85563.0
30	103470.0
31	122316.0
32	157119.0
33	209987.0
34	289606.0
35	424116.0
36	719274.0
37	1293987.0
38	1750394.0
39	672665.0
40	1454.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.884891407628196	20.524491629357275	14.738016239990282	22.852600723024246
2	15.353441835029152	20.522564296112577	40.42531329672107	23.698680572137196
3	18.101916348528235	25.64075347546435	30.53082914313911	25.726501032868303
4	12.713031103738848	17.561266492277174	39.05339809190309	30.67230431208089
5	13.20050014626367	37.793730795672815	35.4506006977216	13.555168360341922
6	30.142119384472814	38.21442093844821	18.1420411327036	13.501418544375376
7	26.44138674344162	32.72227756375514	22.97645388095466	17.85988181184858
8	24.368198542295836	36.28427926980775	21.010395558030506	18.337126629865914
9	24.511467587474936	17.52745973004248	22.123305987595216	35.83776669488737
10-11	23.328470000383582	27.368194357085716	29.321746061308808	19.981589581221897
12-13	23.976613907721774	25.0107777553292	29.08530459511726	21.927303741831764
14-15	21.56825895534332	27.30335877460437	26.282953126331034	24.845429143721283
16-17	21.083890703742465	30.362055329635986	26.985660003690988	21.568393962930568
18-19	20.975893606377642	29.328812718619073	28.91588814559419	20.779405529409093
20-21	22.10650633620409	28.643328908008808	28.368319996253522	20.881844759533575
22-23	21.861226549654937	28.50688962322472	28.352678585870695	21.279205241249656
24-25	21.611419798351267	28.598785017703726	28.389011549017702	21.400783634927308
26-27	21.382490246780673	28.565530065732087	28.472214627356703	21.57976506013054
28-29	21.4438864436935	28.55332145580197	28.604750021508803	21.398042078995726
30-31	21.746512155418056	28.907993765840057	28.120266757963403	21.22522732077849
32-33	21.3871575506932	28.67268584669569	28.494946450576762	21.445210152034345
34-35	21.607780109888985	28.332971462861188	28.513023524456145	21.546224902793686
36-37	21.828405456864523	28.684007431095992	28.06213838105625	21.425448730983234
38-39	21.644916405558003	28.796994456154117	28.198564751597637	21.35952438669024
40-41	21.639053591702368	28.479517029218254	28.301214359107078	21.5802150199723
42-43	21.51294522684474	28.562088180813415	28.24810625753357	21.676860334808275
44-45	21.631709837455272	28.668741596514856	28.15043793447294	21.549110631556935
46-47	21.658609517911366	28.700411176482373	28.02343351619826	21.61754578940801
48-49	21.538603151445873	28.564702928103237	28.127207092428574	21.76948682802232
50-51	21.531053048878267	28.54182555748437	28.093809292815642	21.833312100821722
52-53	21.560933792080377	28.592071748160507	28.03983458610711	21.807159873652008
54-55	21.663318734913126	28.80121449818765	27.924577567522185	21.610889199377038
56-57	21.563385649906426	28.55859871119933	27.98166105787155	21.8963545810227
58-59	21.478824372238687	28.326063279122792	28.211802333184487	21.983310015454034
60-61	21.66537016526927	28.66130967693239	27.869406623402032	21.80391353439631
62-63	21.479560507546555	28.649689292108555	27.978453026247724	21.89229717409717
64-65	21.577497120891735	28.42385095655724	28.03950252090483	21.959149401646194
66-67	21.428198411700766	28.491203535962264	27.94513324558744	22.135464806749532
68-69	21.745536926057916	28.612907767811397	27.897219973940818	21.74433533218987
70-71	21.70500655324956	28.406992618485017	27.98048060655652	21.907520221708904
72-73	21.54502043442251	28.522403608191517	28.088187535741753	21.84438842164422
74-75	21.579936351705125	28.563701368498425	27.94575182218163	21.91061045761482
76-77	21.626717118875707	28.577436253868022	27.760537783085077	22.03530884417119
78-79	21.733031885760067	28.553905833654454	27.91572387276998	21.797338407815495
80-81	21.5793984367947	28.464469847737384	27.975571489358686	21.980560226109233
82-83	21.523326198139838	28.510267569370956	28.09479550395773	21.871610728531472
84-85	21.59143275994057	28.382706760025762	27.923965336250557	22.10189514378311
86-87	21.731393238476105	28.530822129368723	27.844326552028896	21.893458080126273
88-89	21.55770463473057	28.433152441576564	27.888089381591747	22.121053542101123
90-91	21.500431769450596	28.32848184839073	27.952271278307933	22.21881510385074
92-93	21.59563263248576	28.468798823743708	27.813608974114583	22.121959569655946
94-95	21.545474124119437	28.25817063341434	27.958684983707414	22.237670258758804
96-97	21.63007578980351	28.32822699368499	27.85123733322365	22.190459883287847
98-99	21.60498491258449	28.346564523434925	27.799316924454782	22.2491336395258
100	21.69994888979327	28.265731176332675	27.74909910173581	22.285220832138247
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3474.0
1	2598.0
2	1963.5
3	2208.0
4	2052.0
5	1816.0
6	1645.0
7	1564.0
8	1651.5
9	1677.5
10	1511.0
11	1366.5
12	1363.5
13	1472.5
14	1577.0
15	1729.0
16	2094.5
17	2613.0
18	3375.0
19	4463.5
20	6126.5
21	8573.5
22	12373.5
23	18255.5
24	25866.5
25	35505.0
26	48567.0
27	64236.0
28	82887.5
29	104080.0
30	123770.0
31	141778.5
32	158583.5
33	173674.5
34	187134.0
35	197710.0
36	206477.0
37	214610.0
38	223107.5
39	227455.5
40	229119.0
41	230326.0
42	227643.0
43	224313.5
44	220315.5
45	215806.0
46	212039.5
47	205776.0
48	201222.5
49	199501.5
50	194306.0
51	186069.0
52	175295.5
53	165975.0
54	159975.0
55	153128.0
56	141187.5
57	126873.0
58	114660.0
59	104296.5
60	92235.5
61	78486.0
62	66255.5
63	55058.0
64	45376.5
65	36928.0
66	29412.5
67	23190.5
68	18362.5
69	14385.0
70	11022.5
71	8445.0
72	6404.0
73	4812.0
74	3659.0
75	2766.0
76	1930.5
77	1330.0
78	956.5
79	638.5
80	409.0
81	259.0
82	178.0
83	141.5
84	110.5
85	83.0
86	64.0
87	51.5
88	39.5
89	33.0
90	27.5
91	20.5
92	20.5
93	20.0
94	19.0
95	15.0
96	11.0
97	11.0
98	10.5
99	16.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0788778490021359
2	0.013354246257537948
3	0.018021301177992388
4	0.011059225851109859
5	0.009488138056105393
6	0.0069466725053628775
7	0.020193099012263262
8	0.00927249855483027
9	0.009688374735860865
10-11	0.013616094223372028
12-13	0.014440145174673388
14-15	0.009765388843459124
16-17	0.012676522110673279
18-19	0.008848920963039852
20-21	0.013723913974009587
22-23	0.013677705509450633
24-25	0.01584950334372151
26-27	0.005514210104035277
28-29	0.012145124768245297
30-31	0.010235174899808496
32-33	0.0121759304112846
34-35	0.008987546356716716
36-37	0.010427710168804142
38-39	0.019184214202726084
40-41	0.012738133396751885
42-43	0.008348329263651174
44-45	0.020555065317975078
46-47	0.018283149143826463
48-49	0.01801359976723256
50-51	0.010781975063756128
52-53	0.011120837137188463
54-55	0.011505907675179753
56-57	0.02034712722745978
58-59	0.002464451443144258
60-61	0.007501174080070335
62-63	0.01557225255636778
64-65	0.024521291859285366
66-67	0.013015384184105614
68-69	0.014463249406952865
70-71	0.025322238578307253
72-73	0.02016229336922396
74-75	0.020547363907215253
76-77	0.009688374735860865
78-79	0.011575220372018188
80-81	0.01994665386794884
82-83	0.012098916303686341
84-85	0.011575220372018188
86-87	0.016758269813380956
88-89	0.014755903015826246
90-91	0.015487537038009697
92-93	0.008232808102253786
94-95	0.015726280771564297
96-97	0.013500573061974639
98-99	0.00998872975549407
100	0.007131506363598697
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6492317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.39624722749696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.32026056028207	45.49061078038284
2	14.663204323521114	17.71205026941367
3	4.624158336393372	8.378454303117152
4	1.9126479583983298	4.620670358183713
5	0.9899833427185543	2.9895639358966823
6	0.5961470961250486	2.160302844091369
7	0.37384261706146993	1.5805083786953307
8	0.26880014518373624	1.2987616018643207
9	0.19783858587833197	1.0753837329461513
>10	0.9846465267140133	10.469820731378409
>50	0.04998828838946418	2.059895726494161
>100	0.01791988114087881	1.8355741543140287
>500	4.3453855417471246E-4	0.17642358267809835
>1k	1.2779963942150443E-4	0.15197960054412252
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.540282151965161E-5	0.0
5	0.0	0.0	0.0	1.540282151965161E-5	0.0
6	0.0	0.0	0.0	1.540282151965161E-5	0.0
7	0.0	0.0	0.0	1.540282151965161E-5	0.0
8	0.0	0.0	0.0	1.540282151965161E-5	0.0
9	0.0	0.0	0.0	3.080564303930322E-5	0.0
10-11	0.0	0.0	0.0	3.080564303930322E-5	0.0
12-13	0.0	0.0	0.0	3.080564303930322E-5	0.0
14-15	0.0	0.0	0.0	6.161128607860645E-5	0.0
16-17	0.0	0.0	0.0	7.701410759825805E-5	0.0
18-19	0.0	0.0	0.0	9.241692911790968E-5	0.0
20-21	0.0	0.0	0.0	1.3092398291703872E-4	0.0
22-23	0.0	7.701410759825806E-6	0.0	2.541465550742516E-4	0.0
24-25	0.0	1.540282151965161E-5	0.0	5.159945209083289E-4	0.0
26-27	0.0	1.540282151965161E-5	0.0	0.0010936003278952645	0.0
28-29	0.0	1.540282151965161E-5	0.0	0.0031498770007687546	0.0
30-31	0.0	1.540282151965161E-5	0.0	0.008949039302917586	0.0
32-33	0.0	1.540282151965161E-5	0.0	0.023789657837101914	0.0
34-35	0.0	1.540282151965161E-5	0.0	0.04883464562805544	0.0
36-37	0.0	1.540282151965161E-5	0.0	0.0876497558575775	0.0
38-39	0.0	1.540282151965161E-5	0.0	0.13900276280409596	0.0
40-41	0.0	1.540282151965161E-5	0.0	0.19270470003236134	0.0
42-43	0.0	1.540282151965161E-5	0.0	0.24847831675501983	0.0
44-45	0.0	1.540282151965161E-5	0.0	0.3213490653644916	0.0
46-47	0.0	1.540282151965161E-5	0.0	0.4097689622980517	0.0
48-49	0.0	1.540282151965161E-5	0.0	0.5022243984697605	0.0
50-51	0.0	3.080564303930322E-5	0.0	0.5968516324757402	0.0
52-53	0.0	4.620846455895484E-5	0.0	0.6931269683843226	0.0
54-55	0.0	4.620846455895484E-5	0.0	0.8046125905435609	0.0
56-57	0.0	4.620846455895484E-5	0.0	0.9384631095493334	0.0
58-59	0.0	4.620846455895484E-5	0.0	1.0847976153967838	0.0
60-61	1.540282151965161E-5	4.620846455895484E-5	0.0	1.2391877968990115	0.0
62-63	1.540282151965161E-5	4.620846455895484E-5	0.0	1.3945021476924184	0.0
64-65	1.540282151965161E-5	4.620846455895484E-5	0.0	1.5659124469738614	0.0
66-67	1.540282151965161E-5	4.620846455895484E-5	0.0	1.767812631453455	0.0
68-69	1.540282151965161E-5	4.620846455895484E-5	0.0	1.9943419275429712	0.0
70-71	1.540282151965161E-5	4.620846455895484E-5	0.0	2.23845354439717	0.0
72-73	1.540282151965161E-5	4.620846455895484E-5	0.0	2.4924383698454653	0.0
74-75	1.540282151965161E-5	4.620846455895484E-5	0.0	2.751236577018651	0.0
76-77	1.540282151965161E-5	5.390987531878064E-5	0.0	3.0362657892398044	0.0
78-79	1.540282151965161E-5	6.161128607860645E-5	0.0	3.35876236480751	0.0
80-81	1.540282151965161E-5	6.161128607860645E-5	0.0	3.7015136506735575	0.0
82-83	1.540282151965161E-5	6.161128607860645E-5	0.0	4.058504845034523	0.0
84-85	1.540282151965161E-5	6.161128607860645E-5	0.0	4.419531578633637	0.0
86-87	1.540282151965161E-5	6.161128607860645E-5	0.0	4.803939795299582	0.0
88	1.540282151965161E-5	6.161128607860645E-5	0.0	5.117402616046013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6485	0.0	44.38109	1
GTATCAA	11370	0.0	36.811714	1
TCAACGC	11835	0.0	33.95235	4
ATCAACG	11890	0.0	33.677494	3
CAACGCA	13040	0.0	30.708416	5
TATCAAC	13505	0.0	30.20697	2
AACGCAG	13655	0.0	29.293419	6
ACGCAGA	15210	0.0	25.989565	7
CGCAGAG	15595	0.0	25.074173	8
GTGGTAT	3835	0.0	23.176771	1
GTACATG	17355	0.0	22.51816	1
TACATGG	17700	0.0	21.268715	2
GCAGAGT	18385	0.0	20.527872	9
ACATGGG	17960	0.0	19.887915	3
TGGTATC	4160	0.0	19.883892	2
CAGAGTA	17450	0.0	18.207487	10-11
AGAGTAC	16000	0.0	17.49285	10-11
GAGTACT	8025	0.0	17.21793	12-13
CATGGGG	13180	0.0	16.7949	4
GAGTACA	13075	0.0	15.538122	1
>>END_MODULE