FastQCFastQC Report
Wed 25 May 2016
SRR2049441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6492317
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218680.33682890099174145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210230.32381351680763587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117590.1811217782495833No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125800.060.8713531
GTATCAA230700.040.4558371
GTGGTAT46300.034.054141
ATCAACG281000.032.8710333
TCAACGC284150.032.539724
TATCAAC289250.032.0797422
CAACGCA295050.031.3216765
AACGCAG304750.030.3815146
TGGTATC51700.029.4585652
ACGCAGA343000.026.829067
CGCAGAG348800.026.3290358
GCAGAGT381500.023.9613869
GAGTACT286900.022.27078412-13
CAGAGTA373900.021.97844910-11
GTACATG175200.021.08831
GTACTTT310100.020.54398214-15
AGAGTAC356900.020.29942910-11
TACATGG172550.020.1592252
ACATGGG171950.019.51883
AGTACTT298400.019.18383212-13
Produced by FastQC (version 0.11.5)