##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049441_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6492317
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98529138364624	33.0	31.0	34.0	30.0	34.0
2	32.154240466077056	33.0	31.0	34.0	30.0	34.0
3	32.11278577432371	34.0	31.0	34.0	30.0	34.0
4	35.83214297761493	37.0	35.0	37.0	35.0	37.0
5	35.88580271111223	37.0	35.0	37.0	35.0	37.0
6	35.94290451313452	37.0	35.0	37.0	35.0	37.0
7	35.933995367139346	37.0	35.0	37.0	35.0	37.0
8	35.946182541610334	37.0	35.0	37.0	35.0	37.0
9	37.671748160171475	39.0	37.0	39.0	35.0	39.0
10-11	37.64027834746825	39.0	37.0	39.0	35.0	39.0
12-13	37.6177835278222	39.0	37.0	39.0	35.0	39.0
14-15	38.924178840928434	40.0	38.0	41.0	35.0	41.0
16-17	38.933225303077464	40.0	38.0	41.0	35.5	41.0
18-19	39.01224585922098	40.0	38.5	41.0	36.0	41.0
20-21	39.03985949854266	40.0	39.0	41.0	35.5	41.0
22-23	39.01106777441706	40.0	39.0	41.0	35.0	41.0
24-25	38.988158772900334	40.0	39.0	41.0	35.0	41.0
26-27	38.88850921789555	40.0	38.0	41.0	35.0	41.0
28-29	38.805730142258916	40.0	38.0	41.0	35.0	41.0
30-31	38.687843800603076	40.0	38.0	41.0	35.0	41.0
32-33	38.545033922403974	40.0	38.0	41.0	34.5	41.0
34-35	38.42374417330515	40.0	38.0	41.0	34.0	41.0
36-37	38.28579958433946	40.0	38.0	41.0	34.0	41.0
38-39	38.17192090281482	40.0	38.0	41.0	34.0	41.0
40-41	38.07175427509162	40.0	38.0	41.0	34.0	41.0
42-43	37.92063003085031	40.0	38.0	41.0	33.0	41.0
44-45	37.73483072376164	40.0	37.5	41.0	33.0	41.0
46-47	37.47603313270132	40.0	37.0	41.0	33.0	41.0
48-49	37.21603689407033	40.0	37.0	41.0	32.0	41.0
50-51	37.04845227058383	40.0	37.0	41.0	32.0	41.0
52-53	36.92813659591791	40.0	36.5	41.0	31.0	41.0
54-55	36.78082408791808	40.0	36.0	41.0	31.0	41.0
56-57	36.62865815085739	39.5	36.0	41.0	31.0	41.0
58-59	36.450989839220725	39.0	35.0	41.0	31.0	41.0
60-61	36.26158234417697	39.0	35.0	41.0	31.0	41.0
62-63	36.04356680365423	39.0	35.0	41.0	30.0	41.0
64-65	35.82159943822829	38.5	35.0	40.5	30.0	41.0
66-67	35.55823683594008	38.0	35.0	40.0	30.0	41.0
68-69	35.26807147586909	37.0	35.0	40.0	29.5	41.0
70-71	34.98111960645175	37.0	35.0	39.5	29.0	41.0
72-73	34.65202269082055	36.0	34.0	39.0	29.0	41.0
74-75	34.330601694279565	36.0	34.0	39.0	29.0	41.0
76-77	34.014752668423306	35.0	34.0	38.5	29.0	40.0
78-79	33.70965427288902	35.0	34.0	37.0	29.0	39.0
80-81	33.40407284487187	35.0	34.0	37.0	29.0	39.0
82-83	33.13245217385411	35.0	34.0	36.5	28.5	39.0
84-85	32.84946760917558	35.0	34.0	36.0	28.0	37.5
86-87	32.604708226662375	35.0	34.0	36.0	27.5	37.0
88-89	32.3870441015126	35.0	34.0	35.5	27.0	37.0
90-91	32.19688348551064	35.0	33.0	35.0	27.0	36.0
92-93	32.03353394481508	35.0	33.0	35.0	27.0	36.0
94-95	31.876472606004914	35.0	33.0	35.0	26.0	36.0
96-97	31.73209749308298	35.0	33.0	35.0	26.0	36.0
98-99	31.597916044456856	35.0	33.0	35.0	25.5	35.5
100	31.49207455520117	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3351371979671498
1101	2	0.35888785554448077
1101	3	0.4382542903417459
1101	4	0.24731166659773152
1101	5	0.2366463836788668
1101	6	0.2405707603381444
1101	7	0.28670603405956285
1101	8	0.2766256067063395
1101	9	0.31903815273594915
1101	10-11	0.31989753101252916
1101	12-13	0.2831785176601329
1101	14-15	0.40655085147315617
1101	16-17	0.4153622682896767
1101	18-19	0.4466150729349465
1101	20-21	0.42485532141291316
1101	22-23	0.4532473187072057
1101	24-25	0.4105427568353761
1101	26-27	0.4175314746056671
1101	28-29	0.43794137504644937
1101	30-31	0.4329372815359136
1101	32-33	0.4083045376179655
1101	34-35	0.4005901225745916
1101	36-37	0.40471472679747933
1101	38-39	0.37341043833792753
1101	40-41	0.37281156006950766
1101	42-43	0.4032951589921794
1101	44-45	0.3572585437458926
1101	46-47	0.3838654768170713
1101	48-49	0.3484750768128535
1101	50-51	0.33706271358425965
1101	52-53	0.2947789766335802
1101	54-55	0.2850018572673676
1101	56-57	0.31714915325500215
1101	58-59	0.3746464395982798
1101	60-61	0.2822786299823079
1101	62-63	0.316550929666505
1101	64-65	0.29187848609292644
1101	66-67	0.3107808825864069
1101	68-69	0.3019525384621815
1101	70-71	0.27141721931738516
1101	72-73	0.3010513383313693
1101	74-75	0.2903220331799332
1101	76-77	0.3003104846782705
1101	78-79	0.2980270254904198
1101	80-81	0.27658824004892324
1101	82-83	0.2524735090541981
1101	84-85	0.2920062460111126
1101	86-87	0.21885166777370557
1101	88-89	0.22517007286803192
1101	90-91	0.25777096065035465
1101	92-93	0.27656284790420216
1101	94-95	0.28253927548836444
1101	96-97	0.23525304831364124
1101	98-99	0.3079453412726565
1101	100	0.3045396519947836
1102	1	0.30156000339626843
1102	2	0.3131726803197523
1102	3	0.33336787251139555
1102	4	0.2499827325520272
1102	5	0.2353929060167559
1102	6	0.19954462212161417
1102	7	0.20897821307173103
1102	8	0.2550234667016582
1102	9	0.2586636467674026
1102	10-11	0.2842930435537454
1102	12-13	0.24394972946765137
1102	14-15	0.3454842379563985
1102	16-17	0.35662427208227854
1102	18-19	0.36312402807580924
1102	20-21	0.3585399488331191
1102	22-23	0.3041609871939457
1102	24-25	0.28012786569482984
1102	26-27	0.22735393229432077
1102	28-29	0.2806158426976211
1102	30-31	0.29533998167465825
1102	32-33	0.31091409749350163
1102	34-35	0.362217784318716
1102	36-37	0.36335477937056737
1102	38-39	0.3428826165260688
1102	40-41	0.3665574077332252
1102	42-43	0.32062193768565805
1102	44-45	0.3829354453742795
1102	46-47	0.371453635100778
1102	48-49	0.28443777248123325
1102	50-51	0.2677535387365211
1102	52-53	0.2988584120497464
1102	54-55	0.37684368170993565
1102	56-57	0.36152592427470154
1102	58-59	0.3441440339470816
1102	60-61	0.2552374444716321
1102	62-63	0.30377432613079947
1102	64-65	0.26191898714407813
1102	66-67	0.28214371926684834
1102	68-69	0.27006911082896323
1102	70-71	0.2330710697498546
1102	72-73	0.2646354135487812
1102	74-75	0.2568310784490251
1102	76-77	0.24688413128702535
1102	78-79	0.28222955546706885
1102	80-81	0.2425801681647073
1102	82-83	0.225090055155043
1102	84-85	0.18040048542670917
1102	86-87	0.21340760083779742
1102	88-89	0.21028122415058448
1102	90-91	0.22704962065474632
1102	92-93	0.23611871916607186
1102	94-95	0.23760922014448127
1102	96-97	0.19251313469253617
1102	98-99	0.17534567747670593
1102	100	0.1939957307060851
1103	1	0.26028777697013794
1103	2	0.2920717960643273
1103	3	0.35170603137453327
1103	4	0.18413163421601553
1103	5	0.1816253566885706
1103	6	0.202851116184938
1103	7	0.20790790976467122
1103	8	0.19652288353379532
1103	9	0.22912366400542084
1103	10-11	0.24836516111655982
1103	12-13	0.2363178163781896
1103	14-15	0.33872844642407074
1103	16-17	0.32843155751654507
1103	18-19	0.3040870231159971
1103	20-21	0.2948593427210753
1103	22-23	0.3160757821248623
1103	24-25	0.3546775443448311
1103	26-27	0.30821751883049586
1103	28-29	0.3218021729001421
1103	30-31	0.3328018924649143
1103	32-33	0.29421827741443707
1103	34-35	0.31001352633656154
1103	36-37	0.2823517266551363
1103	38-39	0.280719800919087
1103	40-41	0.2831360612789169
1103	42-43	0.28576981267933377
1103	44-45	0.24066736567613134
1103	46-47	0.2476095108706886
1103	48-49	0.2349823430828053
1103	50-51	0.28025932160202416
1103	52-53	0.30734423172635417
1103	54-55	0.26586820145814727
1103	56-57	0.22042415540522597
1103	58-59	0.21047914965819103
1103	60-61	0.17889557560889102
1103	62-63	0.21927097117223582
1103	64-65	0.16539389658071713
1103	66-67	0.18824034382667776
1103	68-69	0.16656055019564775
1103	70-71	0.13985329510408206
1103	72-73	0.1444197510186811
1103	74-75	0.1495066733420103
1103	76-77	0.2042304176413765
1103	78-79	0.16568339754114447
1103	80-81	0.13432808882359382
1103	82-83	0.12514953748048896
1103	84-85	0.1347391587208122
1103	86-87	0.10846814211001998
1103	88-89	0.13831727336859245
1103	90-91	0.14326875031406416
1103	92-93	0.15731318024683816
1103	94-95	0.12303720598587731
1103	96-97	0.14327564610385934
1103	98-99	0.18716259703540317
1103	100	0.12501346721781204
1104	1	0.21505490749980538
1104	2	0.2386865448418476
1104	3	0.29574834087169677
1104	4	0.1734239661981789
1104	5	0.2111368321508067
1104	6	0.14671417787161545
1104	7	0.13944371531103883
1104	8	0.1355580753648553
1104	9	0.22301466196495312
1104	10-11	0.20165154393701812
1104	12-13	0.1794098929975334
1104	14-15	0.28536238200555175
1104	16-17	0.25852104066687076
1104	18-19	0.22653454002030315
1104	20-21	0.22898609443392814
1104	22-23	0.25428362727918596
1104	24-25	0.25569314476117455
1104	26-27	0.23026010291903276
1104	28-29	0.25854134186878497
1104	30-31	0.27053799393060984
1104	32-33	0.211073947320763
1104	34-35	0.21132744368193812
1104	36-37	0.2391390481200517
1104	38-39	0.2355957361937726
1104	40-41	0.23556676751314853
1104	42-43	0.2795731310600402
1104	44-45	0.2049517712478348
1104	46-47	0.16371171254003514
1104	48-49	0.15579855222839
1104	50-51	0.14001342872319356
1104	52-53	0.19298926380535164
1104	54-55	0.15787948904836213
1104	56-57	0.16458815230098622
1104	58-59	0.17625268859230658
1104	60-61	0.12659994638310934
1104	62-63	0.11916508888104715
1104	64-65	0.03992692424526467
1104	66-67	0.1019758827498265
1104	68-69	0.06091297379256133
1104	70-71	0.07325050095195706
1104	72-73	0.09259393308349928
1104	74-75	0.04994250663379063
1104	76-77	0.06654012713038071
1104	78-79	0.038264190268080256
1104	80-81	0.016245936618858536
1104	82-83	-0.041749939412198955
1104	84-85	-0.015096641123783172
1104	86-87	0.004886215709575481
1104	88-89	0.03631320364290502
1104	90-91	0.01652378892266171
1104	92-93	0.008215693628699228
1104	94-95	-0.007353778971715741
1104	96-97	0.018054285137392156
1104	98-99	0.03541619827385745
1104	100	-0.006441845600452467
1105	1	0.19582302315195932
1105	2	0.20506440243184443
1105	3	0.30094218192161293
1105	4	0.19838482788130563
1105	5	0.2016933753776371
1105	6	0.16232675148824427
1105	7	0.17375810317928142
1105	8	0.16011513197315708
1105	9	0.19651534077125632
1105	10-11	0.16874583173427027
1105	12-13	0.20428560373257199
1105	14-15	0.24235172575811248
1105	16-17	0.24807045529565386
1105	18-19	0.23049076952165137
1105	20-21	0.20104402114875342
1105	22-23	0.20377615731512577
1105	24-25	0.2444566410116522
1105	26-27	0.22543552630831698
1105	28-29	0.2470421585052378
1105	30-31	0.21306591227188676
1105	32-33	0.22006581981235485
1105	34-35	0.22030820379713845
1105	36-37	0.19981032505703666
1105	38-39	0.20918627108534338
1105	40-41	0.22593117379790328
1105	42-43	0.2268357871197324
1105	44-45	0.26382279163161826
1105	46-47	0.24231458824424124
1105	48-49	0.2137736136258468
1105	50-51	0.15263776455239508
1105	52-53	0.16012163971682014
1105	54-55	0.18869766197309445
1105	56-57	0.2048012405713706
1105	58-59	0.23844856179155016
1105	60-61	0.18050088622423743
1105	62-63	0.1927590222456388
1105	64-65	0.16952138325563482
1105	66-67	0.1907685497046856
1105	68-69	0.18867947840804078
1105	70-71	0.17455286687946625
1105	72-73	0.16837082086506427
1105	74-75	0.14882436316057834
1105	76-77	0.15946531436016897
1105	78-79	0.10948674595074692
1105	80-81	0.10341775544895881
1105	82-83	0.06807840446031577
1105	84-85	0.14612773873081863
1105	86-87	0.12380601363739885
1105	88-89	0.104745131569139
1105	90-91	0.0897602350184954
1105	92-93	0.09166467724742233
1105	94-95	0.10892012144890018
1105	96-97	0.15214608161894816
1105	98-99	0.15537437585100378
1105	100	0.11767050390607992
1106	1	0.16175767892981696
1106	2	0.14972890202722056
1106	3	0.2459701293533314
1106	4	0.1501728634105035
1106	5	0.1552193784900524
1106	6	0.1729597356954642
1106	7	0.16606692734807638
1106	8	0.1254165467963375
1106	9	0.17088488490156806
1106	10-11	0.18525782834124982
1106	12-13	0.17831263338533176
1106	14-15	0.22108162987072433
1106	16-17	0.21671754596659554
1106	18-19	0.2134994324350643
1106	20-21	0.19964505448380265
1106	22-23	0.18471982304301093
1106	24-25	0.16767151883154696
1106	26-27	0.15352020280246848
1106	28-29	0.18540753144318955
1106	30-31	0.1813983557593346
1106	32-33	0.1649716313183731
1106	34-35	0.16685958888386665
1106	36-37	0.20264563037963512
1106	38-39	0.1990152514092145
1106	40-41	0.21328506262616997
1106	42-43	0.24284901726557706
1106	44-45	0.24030292151644517
1106	46-47	0.18797546419244782
1106	48-49	0.20978050007052929
1106	50-51	0.2028718564001437
1106	52-53	0.1736149133391578
1106	54-55	0.21685682811700246
1106	56-57	0.22303193225843643
1106	58-59	0.13828227560883022
1106	60-61	0.12155444446658237
1106	62-63	0.14003910637636352
1106	64-65	0.15804218487512145
1106	66-67	0.18654271902966002
1106	68-69	0.18159391741781405
1106	70-71	0.19257363847199827
1106	72-73	0.1892731934461338
1106	74-75	0.2033654483551075
1106	76-77	0.18667213601064958
1106	78-79	0.1526907647346576
1106	80-81	0.12746299860381782
1106	82-83	0.167275512856925
1106	84-85	0.1693477887731163
1106	86-87	0.16008232945658563
1106	88-89	0.1330927637184942
1106	90-91	0.15738060226995998
1106	92-93	0.17892837887346502
1106	94-95	0.1604668099866693
1106	96-97	0.15278562615115376
1106	98-99	0.1639207936747944
1106	100	0.1288226069078071
1107	1	0.09936338216747487
1107	2	0.1435778983030147
1107	3	0.20555483914777284
1107	4	0.09756962089074506
1107	5	0.1088867214507161
1107	6	0.10249958724913455
1107	7	0.10361417917516746
1107	8	0.12326077397703017
1107	9	0.09443069301467943
1107	10-11	0.058339491965014645
1107	12-13	0.08113501701444648
1107	14-15	0.16747062786821232
1107	16-17	0.13640587739229915
1107	18-19	0.14468523120186205
1107	20-21	0.16673841855696736
1107	22-23	0.17966126057265797
1107	24-25	0.16843063305916672
1107	26-27	0.18123377578742605
1107	28-29	0.2238325521738247
1107	30-31	0.15332032631143022
1107	32-33	0.17242797253386755
1107	34-35	0.17219448772780765
1107	36-37	0.2013368862110383
1107	38-39	0.1919169224466657
1107	40-41	0.2270302092448162
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2216	62-63	-0.5930119282398181
2216	64-65	-0.6435589792950722
2216	66-67	-0.5989120832134844
2216	68-69	-0.6007712811787158
2216	70-71	-0.6074389085456531
2216	72-73	-0.5548549404296566
2216	74-75	-0.4982616544200482
2216	76-77	-0.43717663293465847
2216	78-79	-0.4110709390466525
2216	80-81	-0.43035225288673473
2216	82-83	-0.4399157284347197
2216	84-85	-0.5567959076011917
2216	86-87	-0.4571606939216082
2216	88-89	-0.4997309610088756
2216	90-91	-0.47659023339358697
2216	92-93	-0.522620185918548
2216	94-95	-0.5067796633623693
2216	96-97	-0.49478584529323655
2216	98-99	-0.5834668632177866
2216	100	-0.6272557897192037
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	460.0
3	56.0
4	65.0
5	155.0
6	452.0
7	1202.0
8	2599.0
9	4269.0
10	5404.0
11	7547.0
12	10504.0
13	15054.0
14	18365.0
15	24208.0
16	30472.0
17	41849.0
18	43785.0
19	23602.0
20	16379.0
21	15286.0
22	15997.0
23	17104.0
24	19896.0
25	24190.0
26	30988.0
27	39974.0
28	52357.0
29	68807.0
30	89921.0
31	116812.0
32	152858.0
33	201260.0
34	276254.0
35	408756.0
36	686025.0
37	1309614.0
38	1907250.0
39	810013.0
40	2528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31232071273335	20.588143207408038	15.081237307514167	23.01829877234444
2	15.468637776005083	20.848473665102922	39.89304588793184	23.78984267096015
3	17.671610921031736	25.6958340142664	31.180054824802916	25.452500239898946
4	12.628449904710445	17.924540653205938	38.5125218007685	30.934487641315112
5	12.670018615547956	37.54295717931006	35.62434181621445	14.16268238892754
6	29.75599868213134	38.56903738751484	17.60779421656513	14.067169713788685
7	25.828190459584764	33.32890245500951	22.76333703360449	18.07957005180123
8	24.559814315906017	35.86426848843025	20.588843705567673	18.987073490096062
9	24.596211183156953	16.806773298346336	22.03998972939861	36.5570257890981
10-11	23.36505595768044	27.182329513484937	28.9314123139705	20.52120221486412
12-13	24.41667589552389	24.869857710275085	29.158711751135996	21.554754643065028
14-15	21.72883733188013	26.755455717889316	27.096335560940727	24.419371389289832
16-17	20.48503638993598	29.910300128598156	27.83166626028889	21.772997221176972
18-19	20.279747584722063	28.037632789649674	31.0685075913576	20.61411203427066
20-21	21.337590262459457	27.337150973989715	30.8908206423069	20.434438121243925
22-23	21.080547976939513	27.16979161676794	30.977284380907466	20.772376025385082
24-25	20.9237010454049	27.263956766128334	30.95219318465195	20.86014900381482
26-27	20.738220268665255	27.245827645199704	30.978470398164475	21.037481687970566
28-29	20.780631937719615	27.217116785887075	31.14748555869961	20.8547657176937
30-31	21.10841168106856	27.551188581826796	30.677845829154677	20.662553907949967
32-33	20.72115394242148	27.369658628806942	31.029707883949598	20.87947954482198
34-35	20.946019733786876	27.11380236054401	30.957299219985718	20.9828786856834
36-37	21.224456538397618	27.398531217745532	30.443499601143937	20.933512642712916
38-39	21.10496915045892	27.493073736233153	30.511010167864573	20.89094694544336
40-41	21.04341947566639	27.274646324262967	30.539428373568327	21.142505826502312
42-43	21.06372809584005	27.337636162867586	30.355857546697116	21.242778194595243
44-45	21.22029007517655	27.544095582517	30.066815899470097	21.168798442836355
46-47	21.347478873875076	27.67123817275096	29.660443259317127	21.320839694056836
48-49	21.336904836901834	27.671438409430714	29.408591724649302	21.583065029018144
50-51	21.39768437061838	27.709622003977934	29.19230530487036	21.70038832053333
52-53	21.413749513463376	27.8339843849276	29.100419773094877	21.65184632851415
54-55	21.518003510919137	28.071680726618865	28.922802136741012	21.487513625720986
56-57	21.359754922626237	27.823125395756243	29.002735079017246	21.814384602600274
58-59	21.271196400299	27.504471824157694	29.29641297552168	21.927918800021626
60-61	21.41260200326016	27.8152545539597	28.994794924523866	21.77734851825627
62-63	21.231110557294105	27.78092936620316	29.132719489821586	21.85524058668115
64-65	21.270002681631226	27.608579494809014	29.216310602208733	21.905107221351024
66-67	21.208984404181127	27.64153383145031	29.09669229028712	22.05278947408144
68-69	21.543233332568327	27.761899180215632	28.920037330278237	21.774830156937806
70-71	21.514014180145548	27.577173141730448	28.97186782469186	21.93694485343214
72-73	21.353724718001292	27.70082699289021	29.012254022716387	21.933194266392107
74-75	21.44213691352409	27.756546699737548	28.793064171080985	22.008252215657368
76-77	21.47154089980511	27.76091339963837	28.66071542717338	22.10683027338314
78-79	21.516478631588694	27.81725692075726	28.717236080739745	21.94902836691431
80-81	21.36113609581929	27.69564335745497	28.78438619137473	22.158834355351015
82-83	21.26688486431344	27.75967422225693	28.87677809287001	22.096662820559622
84-85	21.277270685342238	27.674960624487806	28.702124125107346	22.34564456506261
86-87	21.33594391867518	27.808386753140574	28.605625198889882	22.250044129294366
88-89	21.196146071243955	27.673150157941333	28.643586756217072	22.487117014597636
90-91	21.089430801640905	27.600442125721493	28.67160043072755	22.63852664191005
92-93	21.128825847405317	27.76690122388764	28.550336563902167	22.55393636480488
94-95	20.999841119516706	27.548297009927836	28.670064075120354	22.781797795435104
96-97	21.011293802521532	27.67528806339532	28.559954499788404	22.753463634294746
98-99	20.927743721336476	27.70308824581572	28.530359796159892	22.838808236687907
100	21.042205477280888	27.59611410978744	28.44105232822406	22.920628084707616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1298.0
1	959.5
2	1167.0
3	1693.0
4	1113.0
5	724.5
6	1768.0
7	4677.5
8	7946.0
9	9240.0
10	7143.0
11	4351.0
12	3852.5
13	4277.0
14	4914.0
15	5594.0
16	6257.5
17	7090.0
18	8095.0
19	9252.0
20	10905.5
21	13195.0
22	16662.0
23	21888.5
24	28989.5
25	38310.5
26	50484.0
27	65045.0
28	82833.5
29	103021.0
30	122110.5
31	139915.5
32	156153.0
33	171050.5
34	184732.0
35	195416.0
36	203196.5
37	211178.0
38	219947.5
39	224336.0
40	226632.0
41	228315.0
42	226166.0
43	224000.5
44	221359.0
45	216630.0
46	212295.0
47	205470.0
48	200527.5
49	198893.5
50	193711.0
51	184958.5
52	172788.0
53	163271.5
54	157310.0
55	149890.5
56	138157.5
57	123710.5
58	111907.0
59	102902.0
60	90964.5
61	76550.0
62	64132.0
63	53569.0
64	44304.5
65	35570.5
66	28097.5
67	21997.0
68	17267.5
69	13353.5
70	10008.5
71	7566.5
72	5828.5
73	4289.0
74	3174.5
75	2330.5
76	1571.5
77	1080.5
78	703.5
79	421.5
80	246.0
81	122.5
82	56.0
83	35.0
84	20.5
85	7.5
86	5.5
87	3.0
88	1.0
89	1.0
90	0.5
91	2.0
92	1.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.5
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13335762871714366
2	0.0
3	0.0
4	0.0
5	0.014925334052542412
6	2.4644514431442584E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.9253526899564515E-4
82-83	5.853072177467613E-4
84-85	3.1575784115285804E-4
86-87	4.7748746710919997E-4
88-89	5.082931101485032E-4
90-91	7.085297899039742E-4
92-93	2.0793809051529677E-4
94-95	2.3874373355459999E-4
96-97	6.084114500262387E-4
98-99	0.0011013017386550903
100	1.2322257215721292E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6492317.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84677855289523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.07858888321299	42.852077266381876
2	15.21337773797036	17.60089786099835
3	4.873032390675313	8.456676767534415
4	2.1155805842145123	4.895180862634465
5	1.064795700316825	3.079750054015119
6	0.6429040231241578	2.2313955993857157
7	0.39212868728658246	1.5878366936393102
8	0.2817388664954772	1.3038148655926067
9	0.20857474000091758	1.0858839116904702
>10	1.0480134362103881	10.696441957903755
>50	0.05732214540603818	2.2453671770414405
>100	0.02237120593294762	2.2314895418829654
>500	0.0010122418553962632	0.37847615425005093
>1k	4.5281305149247287E-4	0.41705114220991213
>5k	2.66360616610807E-5	0.09560728286466896
>10k+	7.990818498324211E-5	0.8420528619748846
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21868	0.33682890099174145	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21023	0.32381351680763587	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11759	0.1811217782495833	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.540282151965161E-5	0.0
5	0.0	0.0	0.0	1.540282151965161E-5	0.0
6	0.0	0.0	0.0	1.540282151965161E-5	0.0
7	0.0	0.0	0.0	1.540282151965161E-5	0.0
8	0.0	0.0	0.0	1.540282151965161E-5	0.0
9	0.0	0.0	0.0	1.540282151965161E-5	0.0
10-11	0.0	0.0	0.0	1.540282151965161E-5	0.0
12-13	0.0	0.0	0.0	1.540282151965161E-5	0.0
14-15	0.0	0.0	0.0	3.080564303930322E-5	0.0
16-17	0.0	0.0	0.0	3.080564303930322E-5	0.0
18-19	0.0	4.620846455895484E-5	0.0	4.620846455895484E-5	0.0
20-21	0.0	4.620846455895484E-5	0.0	1.078197506375613E-4	0.0
22-23	0.0	4.620846455895484E-5	0.0	2.3874373355459999E-4	0.0
24-25	0.0	4.620846455895484E-5	0.0	4.928902886288517E-4	0.0
26-27	0.0	4.620846455895484E-5	0.0	0.0010627946848559614	0.0
28-29	0.0	4.620846455895484E-5	0.0	0.0031267727684892773	0.0
30-31	0.0	4.620846455895484E-5	0.0	0.008895129427598807	0.0
32-33	0.0	4.620846455895484E-5	0.0	0.02375885219406261	0.0
34-35	0.0	4.620846455895484E-5	0.0	0.04896556961097248	0.0
36-37	0.0	4.620846455895484E-5	0.0	0.0880656320386081	0.0
38-39	0.0	4.620846455895484E-5	0.0	0.1395495629680436	0.0
40-41	0.0	4.620846455895484E-5	0.0	0.19353645239442252	0.0
42-43	0.0	4.620846455895484E-5	0.0	0.2493639789923998	0.0
44-45	0.0	4.620846455895484E-5	0.0	0.32247347133542614	0.0
46-47	0.0	4.620846455895484E-5	0.0	0.41103199366266313	0.0
48-49	0.0	4.620846455895484E-5	0.0	0.5038725003723632	0.0
50-51	0.0	7.701410759825807E-5	0.0	0.5986229569505002	0.0
52-53	0.0	7.701410759825807E-5	0.0	0.695183245057196	0.0
54-55	0.0	7.701410759825807E-5	0.0	0.8066996728594737	0.0
56-57	0.0	7.701410759825807E-5	0.0	0.9405347890437266	0.0
58-59	0.0	7.701410759825807E-5	0.0	1.0870002188740937	0.0
60-61	0.0	7.701410759825807E-5	0.0	1.2417061582174744	0.0
62-63	0.0	7.701410759825807E-5	0.0	1.3972900583874757	0.0
64-65	0.0	7.701410759825807E-5	0.0	1.5684770167568836	0.0
66-67	0.0	7.701410759825807E-5	0.0	1.7710626267941014	0.0
68-69	0.0	7.701410759825807E-5	0.0	1.9979076807247704	0.0
70-71	0.0	7.701410759825807E-5	0.0	2.242681618904314	0.0
72-73	0.0	7.701410759825807E-5	0.0	2.4967973683355265	0.0
74-75	0.0	7.701410759825807E-5	0.0	2.7560114516897434	0.0
76-77	0.0	7.701410759825807E-5	0.0	3.0413641231628095	0.0
78-79	0.0	7.701410759825807E-5	0.0	3.3652469526672837	0.0
80-81	0.0	7.701410759825807E-5	0.0	3.709045630396667	0.0
82-83	0.0	7.701410759825807E-5	0.0	4.0665913263323406	0.0
84-85	0.0	7.701410759825807E-5	0.0	4.429705142247368	0.0
86-87	0.0	7.701410759825807E-5	0.0	4.815599731189959	0.0
88	0.0	7.701410759825807E-5	0.0	5.130587431266834	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12580	0.0	60.871353	1
GTATCAA	23070	0.0	40.455837	1
GTGGTAT	4630	0.0	34.05414	1
ATCAACG	28100	0.0	32.871033	3
TCAACGC	28415	0.0	32.53972	4
TATCAAC	28925	0.0	32.079742	2
CAACGCA	29505	0.0	31.321676	5
AACGCAG	30475	0.0	30.381514	6
TGGTATC	5170	0.0	29.458565	2
ACGCAGA	34300	0.0	26.82906	7
CGCAGAG	34880	0.0	26.329035	8
GCAGAGT	38150	0.0	23.961386	9
GAGTACT	28690	0.0	22.270784	12-13
CAGAGTA	37390	0.0	21.978449	10-11
GTACATG	17520	0.0	21.0883	1
GTACTTT	31010	0.0	20.543982	14-15
AGAGTAC	35690	0.0	20.299429	10-11
TACATGG	17255	0.0	20.159225	2
ACATGGG	17195	0.0	19.5188	3
AGTACTT	29840	0.0	19.183832	12-13
>>END_MODULE