FastQCFastQC Report
Wed 25 May 2016
SRR2049440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401507
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57170.1680725631315767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.041.383821
GTATCAA62100.037.793231
TCAACGC65100.034.2974744
ATCAACG64850.034.1417663
CAACGCA70500.031.8699865
TATCAAC74350.030.2855832
AACGCAG75300.030.0226126
ACGCAGA85400.026.3618627
CGCAGAG84550.026.3466138
ATAACGC3900.024.1068483
TATAACG3950.023.8020482
GTGGTAT20200.023.7494641
GTACCTG24500.021.8848611
GCAGAGT96150.021.7512199
TACCTGG23550.021.1590612
GTACATG82500.020.5806121
TGGTATC21750.020.3165532
TACATGG83900.019.9466292
TCTGTCG11850.019.036328
AGAGTAC85600.018.77723310-11
Produced by FastQC (version 0.11.5)