##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049440_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3401507
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.53530508683357	31.0	31.0	34.0	27.0	34.0
2	30.73071465088856	31.0	31.0	34.0	27.0	34.0
3	30.854306635264898	31.0	31.0	34.0	27.0	34.0
4	34.420144071436575	37.0	35.0	37.0	31.0	37.0
5	34.4564312229844	37.0	35.0	37.0	31.0	37.0
6	34.45742872203409	37.0	35.0	37.0	31.0	37.0
7	34.33698710600919	37.0	35.0	37.0	30.0	37.0
8	34.30506125667241	37.0	35.0	37.0	30.0	37.0
9	35.823395336243614	39.0	37.0	39.0	30.0	39.0
10-11	35.746186910684	39.0	37.0	39.0	30.0	39.0
12-13	35.644610462362714	39.0	37.0	39.0	30.0	39.0
14-15	36.8332043708862	40.0	37.0	41.0	30.0	41.0
16-17	36.80884796062451	40.0	37.0	41.0	30.0	41.0
18-19	36.83086217373652	40.0	37.0	41.0	30.0	41.0
20-21	36.879311140620906	40.0	37.0	41.0	30.0	41.0
22-23	36.838059718824624	40.0	37.0	41.0	30.0	41.0
24-25	36.84240823258632	40.0	37.0	41.0	30.0	41.0
26-27	36.83794065395132	40.0	37.0	41.0	30.0	41.0
28-29	36.81057837011654	40.0	37.0	41.0	30.0	41.0
30-31	36.747829858941934	40.0	37.0	41.0	30.0	41.0
32-33	36.68932652497848	40.0	37.0	41.0	30.0	41.0
34-35	36.61218204166565	40.0	37.0	41.0	30.0	41.0
36-37	36.487301951752556	40.0	37.0	41.0	30.0	41.0
38-39	36.36500218873576	40.0	36.0	41.0	30.0	41.0
40-41	36.23729952635699	40.0	36.0	41.0	29.0	41.0
42-43	36.138018531198085	40.0	36.0	41.0	29.0	41.0
44-45	35.985560811722564	40.0	36.0	41.0	28.5	41.0
46-47	35.85219316026691	40.0	35.0	41.0	28.0	41.0
48-49	35.73884972160869	40.0	35.0	41.0	28.0	41.0
50-51	35.6212450834292	39.0	35.0	41.0	27.0	41.0
52-53	35.499802587500184	39.0	35.0	41.0	27.0	41.0
54-55	35.3445092131223	39.0	35.0	41.0	27.0	41.0
56-57	35.17956996707636	39.0	35.0	41.0	26.5	41.0
58-59	34.994184783391596	39.0	34.5	41.0	26.0	41.0
60-61	34.78516007757738	38.5	34.0	40.0	26.0	41.0
62-63	34.509394806478426	38.0	34.0	40.0	26.0	41.0
64-65	34.25546941399797	37.5	34.0	40.0	25.0	41.0
66-67	34.024393305673044	37.0	34.0	40.0	25.0	41.0
68-69	33.72717504329699	36.5	33.5	40.0	24.5	41.0
70-71	33.403815720502706	36.0	33.0	39.0	24.0	41.0
72-73	33.06422432762891	35.5	33.0	39.0	23.0	41.0
74-75	32.776887126794094	35.0	33.0	38.5	23.0	40.0
76-77	32.46519131079254	35.0	33.0	37.5	22.0	40.0
78-79	32.134498914745734	35.0	32.5	37.0	21.5	39.0
80-81	31.81815515887517	35.0	32.0	36.5	20.5	39.0
82-83	31.538715339994887	35.0	32.0	36.0	20.0	38.5
84-85	31.26263270956079	35.0	32.0	36.0	19.5	37.0
86-87	30.999605615981388	35.0	32.0	35.5	18.0	37.0
88-89	30.778516257646977	35.0	32.0	35.0	17.5	36.5
90-91	30.58201585356138	35.0	32.0	35.0	13.0	36.0
92-93	30.378531339197597	35.0	32.0	35.0	7.0	36.0
94-95	30.1729409935067	35.0	31.0	35.0	2.0	36.0
96-97	29.980359734670543	35.0	31.0	35.0	2.0	35.5
98-99	29.798313806204135	35.0	31.0	35.0	2.0	35.0
100	29.65836201424839	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23913584639093344
1101	2	0.23117263238661323
1101	3	0.34870938574466237
1101	4	0.35045312665246797
1101	5	0.32717764065223776
1101	6	0.3513946499484888
1101	7	0.36156925664198525
1101	8	0.41274550024307644
1101	9	0.5229119510780436
1101	10-11	0.5219974969634293
1101	12-13	0.5291620413947555
1101	14-15	0.6724740898372801
1101	16-17	0.6122924094666544
1101	18-19	0.6261860480478063
1101	20-21	0.6206260754536856
1101	22-23	0.511351432451626
1101	24-25	0.614378552197806
1101	26-27	0.6074462977699113
1101	28-29	0.6328403622124696
1101	30-31	0.5444600750130419
1101	32-33	0.5667696425299411
1101	34-35	0.5247417391096079
1101	36-37	0.5983009663654215
1101	38-39	0.6337333777932699
1101	40-41	0.638480384363497
1101	42-43	0.5694947148657761
1101	44-45	0.5935010908075355
1101	46-47	0.5800951022885954
1101	48-49	0.5589103069065047
1101	50-51	0.5627045305393281
1101	52-53	0.5247647655771388
1101	54-55	0.52826356726856
1101	56-57	0.5697287748080626
1101	58-59	0.4963073293884861
1101	60-61	0.5372399525218015
1101	62-63	0.5972360623619366
1101	64-65	0.5433122146891307
1101	66-67	0.5862337385593932
1101	68-69	0.5011075505118114
1101	70-71	0.5083242314994649
1101	72-73	0.5586527324729147
1101	74-75	0.5896919283369755
1101	76-77	0.6037683920999015
1101	78-79	0.556784421831253
1101	80-81	0.5332132605780018
1101	82-83	0.4290285324490988
1101	84-85	0.5509397742200051
1101	86-87	0.5201504006706301
1101	88-89	0.4828564038468528
1101	90-91	0.4669175250276467
1101	92-93	0.4639420672613497
1101	94-95	0.4838370501361844
1101	96-97	0.4935535992651161
1101	98-99	0.4891048773026583
1101	100	0.5057417331078717
1102	1	0.2742430699713161
1102	2	0.383095377601375
1102	3	0.40224713044087324
1102	4	0.39197793176835205
1102	5	0.4397924075263333
1102	6	0.4095720932635061
1102	7	0.42340996394487007
1102	8	0.4474258537313176
1102	9	0.6057045566205588
1102	10-11	0.6260970735614251
1102	12-13	0.6069976372478294
1102	14-15	0.6882673881225543
1102	16-17	0.6351478618775559
1102	18-19	0.5866358657901429
1102	20-21	0.6068850260177285
1102	22-23	0.5604821067002845
1102	24-25	0.5178328234529488
1102	26-27	0.46356865698219707
1102	28-29	0.60310887902979
1102	30-31	0.5521492739506968
1102	32-33	0.4425593553229348
1102	34-35	0.5097755354552262
1102	36-37	0.5090056176007351
1102	38-39	0.5294362910307271
1102	40-41	0.47334671132433215
1102	42-43	0.5327609467852596
1102	44-45	0.46292064282333456
1102	46-47	0.37681065896603627
1102	48-49	0.41125805286130657
1102	50-51	0.4688282699080091
1102	52-53	0.4267162484375717
1102	54-55	0.44195264538528534
1102	56-57	0.4120022949941671
1102	58-59	0.4448828476955029
1102	60-61	0.49041488886494733
1102	62-63	0.4120550659872677
1102	64-65	0.4325679656634449
1102	66-67	0.44059351523135604
1102	68-69	0.46178652001334797
1102	70-71	0.39330224704439587
1102	72-73	0.4337961656950142
1102	74-75	0.47737142829320334
1102	76-77	0.4007442633439311
1102	78-79	0.4403277616773522
1102	80-81	0.45828810856199453
1102	82-83	0.4351918779348871
1102	84-85	0.40787569711809724
1102	86-87	0.3846283682743845
1102	88-89	0.42192516340516306
1102	90-91	0.4285032135676907
1102	92-93	0.4054723971661929
1102	94-95	0.42761611902980334
1102	96-97	0.4647036883799238
1102	98-99	0.4297990780686618
1102	100	0.4368979082336075
1103	1	0.2657437644792324
1103	2	0.38282540633834117
1103	3	0.3974423019959801
1103	4	0.41314451591026113
1103	5	0.3480533712150802
1103	6	0.43059914395745125
1103	7	0.44536979996429693
1103	8	0.47460195472246625
1103	9	0.5295087092921236
1103	10-11	0.5957567588468962
1103	12-13	0.5447254129240804
1103	14-15	0.6695055323574763
1103	16-17	0.5825291828846133
1103	18-19	0.5809909389337164
1103	20-21	0.5055320667233829
1103	22-23	0.5391227635595257
1103	24-25	0.5194327694265013
1103	26-27	0.5917710995298933
1103	28-29	0.5764746725036218
1103	30-31	0.539937199530236
1103	32-33	0.5545305001117669
1103	34-35	0.5605867592569354
1103	36-37	0.575208262103672
1103	38-39	0.640430258976032
1103	40-41	0.7047704809256814
1103	42-43	0.5907836994706983
1103	44-45	0.6072100603885104
1103	46-47	0.6331665041115215
1103	48-49	0.5439660735071001
1103	50-51	0.5634686118812695
1103	52-53	0.5707192541820305
1103	54-55	0.5468551955391447
1103	56-57	0.5022220386430121
1103	58-59	0.5344121080446698
1103	60-61	0.594390871725551
1103	62-63	0.6169203723416743
1103	64-65	0.6527993778823955
1103	66-67	0.5383103141690526
1103	68-69	0.5364298917177805
1103	70-71	0.5247237387128934
1103	72-73	0.5994672428984629
1103	74-75	0.6407962262645057
1103	76-77	0.46726416835171136
1103	78-79	0.5296976069739117
1103	80-81	0.561497683735972
1103	82-83	0.5534358702190509
1103	84-85	0.47734554527616524
1103	86-87	0.5045851768969136
1103	88-89	0.4729396705774107
1103	90-91	0.48625249357388967
1103	92-93	0.44716146585346905
1103	94-95	0.5000364335382734
1103	96-97	0.5413410159960463
1103	98-99	0.5317641010136036
1103	100	0.4872942745846096
1104	1	0.2034586452855791
1104	2	0.19751613979881455
1104	3	0.2237550362924452
1104	4	0.3066309083562757
1104	5	0.34153424959453105
1104	6	0.35181843439296756
1104	7	0.32020902031050724
1104	8	0.305483341671831
1104	9	0.3668252741233644
1104	10-11	0.3954683680781059
1104	12-13	0.4386177095461079
1104	14-15	0.48411506150198136
1104	16-17	0.42863504850129175
1104	18-19	0.49547980042142115
1104	20-21	0.5051587812448943
1104	22-23	0.5436709879459514
1104	24-25	0.4728696471984293
1104	26-27	0.4630676984309403
1104	28-29	0.41683864942375237
1104	30-31	0.38232754699430416
1104	32-33	0.4243719763725764
1104	34-35	0.44880181755831217
1104	36-37	0.4473385064691797
1104	38-39	0.4623916551095064
1104	40-41	0.42294407336822104
1104	42-43	0.4290897829184459
1104	44-45	0.4064408030980502
1104	46-47	0.38435401450420414
1104	48-49	0.3992687877388619
1104	50-51	0.3941789218825633
1104	52-53	0.35935068125415626
1104	54-55	0.2716561755010858
1104	56-57	0.29107058453200096
1104	58-59	0.3955183284165926
1104	60-61	0.4089498389538022
1104	62-63	0.41546451788422445
1104	64-65	0.39762185918183235
1104	66-67	0.4345248585547239
1104	68-69	0.37256212584340176
1104	70-71	0.36780307055517625
1104	72-73	0.33346767392706056
1104	74-75	0.37195724565691535
1104	76-77	0.3335038907714889
1104	78-79	0.3081550920531555
1104	80-81	0.3623075079632585
1104	82-83	0.36995908179770254
1104	84-85	0.2780417812976239
1104	86-87	0.32761571101115905
1104	88-89	0.3066075787374061
1104	90-91	0.32335775107786446
1104	92-93	0.3189056668575958
1104	94-95	0.28505121605022055
1104	96-97	0.3803253022369155
1104	98-99	0.37939605988594494
1104	100	0.3571603078132597
1105	1	0.1385722244613916
1105	2	0.11180450514187967
1105	3	0.1374562350500952
1105	4	0.11478451499031195
1105	5	0.15761192784789557
1105	6	0.2127079917698751
1105	7	0.19144054255823306
1105	8	0.1905724084020619
1105	9	0.23182167011688648
1105	10-11	0.2572767501335491
1105	12-13	0.3028707768145509
1105	14-15	0.3208994646562431
1105	16-17	0.24694814399136078
1105	18-19	0.1927670464303759
1105	20-21	0.24523678658584913
1105	22-23	0.2605778477666618
1105	24-25	0.28436220920680455
1105	26-27	0.32949812412441304
1105	28-29	0.31818093243238366
1105	30-31	0.399049746901575
1105	32-33	0.3796772475389716
1105	34-35	0.3311433791536942
1105	36-37	0.3643561905406614
1105	38-39	0.3468094694193695
1105	40-41	0.3618937221506613
1105	42-43	0.3369664933872585
1105	44-45	0.2941296462674359
1105	46-47	0.2554109396009139
1105	48-49	0.1619585583958454
1105	50-51	0.15002871050335642
1105	52-53	0.23887694506406376
1105	54-55	0.1997272361208431
1105	56-57	0.18441318177725918
1105	58-59	0.2121169869750119
1105	60-61	0.21043065992528653
1105	62-63	0.24633772700127565
1105	64-65	0.2829619786391717
1105	66-67	0.17717676629919765
1105	68-69	0.2215892315060799
1105	70-71	0.2480771188553632
1105	72-73	0.18374920179326182
1105	74-75	0.25301690464286253
1105	76-77	0.17357261358701948
1105	78-79	0.1658184459508334
1105	80-81	0.13821751481634692
1105	82-83	0.0791786183812917
1105	84-85	0.04347942043510855
1105	86-87	0.07266726823033665
1105	88-89	0.0717350736694975
1105	90-91	0.14372650332056836
1105	92-93	0.1362397944007938
1105	94-95	0.10182403192018086
1105	96-97	0.06180110291879615
1105	98-99	0.11984592082399814
1105	100	0.05681220737289294
1106	1	0.2306961320238905
1106	2	0.23787974417819413
1106	3	0.2873694386459569
1106	4	0.2776509167261807
1106	5	0.26175354134078077
1106	6	0.23395330649134394
1106	7	0.3027356747526255
1106	8	0.2722635490041938
1106	9	0.32473261215196203
1106	10-11	0.3457174688893687
1106	12-13	0.35397072654440365
1106	14-15	0.4509112161954647
1106	16-17	0.43148803519581236
1106	18-19	0.45957988515077375
1106	20-21	0.3719018361501867
1106	22-23	0.3427365483430336
1106	24-25	0.393608118354841
1106	26-27	0.46486202277647237
1106	28-29	0.4310694911504669
1106	30-31	0.3938659362699184
1106	32-33	0.4614272798512431
1106	34-35	0.4205822044405281
1106	36-37	0.39808591496618106
1106	38-39	0.43039780162400376
1106	40-41	0.4978735640617842
1106	42-43	0.4415859040353567
1106	44-45	0.3294485534398035
1106	46-47	0.3558287878244002
1106	48-49	0.3931364402479076
1106	50-51	0.3684656202833452
1106	52-53	0.2955700437597173
1106	54-55	0.32306624420277785
1106	56-57	0.3282070453057315
1106	58-59	0.3789248150606639
1106	60-61	0.37403551605678587
1106	62-63	0.3603095655960047
1106	64-65	0.2672308935247898
1106	66-67	0.31894467310169006
1106	68-69	0.3295172245897646
1106	70-71	0.33930875372497127
1106	72-73	0.37492085050502766
1106	74-75	0.3536358111004958
1106	76-77	0.29786371672479106
1106	78-79	0.2959580691868382
1106	80-81	0.398715390247645
1106	82-83	0.31771115162123564
1106	84-85	0.32788989878172004
1106	86-87	0.2996448161194962
1106	88-89	0.27602118404104914
1106	90-91	0.2415901190541696
1106	92-93	0.2132083186802589
1106	94-95	0.2620616744887485
1106	96-97	0.30140448251376384
1106	98-99	0.2744158505232299
1106	100	0.24464288693111058
1107	1	0.21569433390079595
1107	2	0.2592221244007753
1107	3	0.2608261302577368
1107	4	0.23477414399223306
1107	5	0.2683719752239284
1107	6	0.28692506760562964
1107	7	0.28866580118398133
1107	8	0.28169677093303136
1107	9	0.30473926388849293
1107	10-11	0.36864381310727623
1107	12-13	0.3548080737808803
1107	14-15	0.4203514767430647
1107	16-17	0.33452666538651954
1107	18-19	0.36521156051789205
1107	20-21	0.4195040675813715
1107	22-23	0.38367934956382754
1107	24-25	0.3619812653868024
1107	26-27	0.42185050072023245
1107	28-29	0.35467267044312933
1107	30-31	0.3541584104433255
1107	32-33	0.39741277880181514
1107	34-35	0.3352101006716808
1107	36-37	0.3882186045802385
1107	38-39	0.393261393983785
1107	40-41	0.446929747275135
1107	42-43	0.37807011836107307
1107	44-45	0.35127558059278385
1107	46-47	0.38261384265176446
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2216	94-95	-1.2395353284458324
2216	96-97	-1.3418234346782612
2216	98-99	-1.3607935776985762
2216	100	-1.375561919977983
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	75756.0
3	13537.0
4	8535.0
5	6849.0
6	5670.0
7	5469.0
8	5527.0
9	6349.0
10	7328.0
11	8199.0
12	9281.0
13	10942.0
14	11667.0
15	12327.0
16	12335.0
17	12563.0
18	11916.0
19	10691.0
20	10847.0
21	11698.0
22	13633.0
23	16887.0
24	20357.0
25	24156.0
26	29165.0
27	32827.0
28	39674.0
29	49530.0
30	59055.0
31	68246.0
32	86815.0
33	114860.0
34	158154.0
35	227777.0
36	373739.0
37	663981.0
38	852707.0
39	311809.0
40	649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15472727101545	19.808010516553924	13.133747122540921	22.9035150898897
2	16.612874344723075	21.016935099026977	38.943435881287	23.426754674962947
3	17.927564404332152	26.043776097894174	30.6502372756364	25.378422222137274
4	12.896898668673847	18.288122736039895	38.71671684621536	30.098261749070893
5	12.63145081834906	36.89746226085852	34.603962099002786	15.867124821789632
6	28.884949840839003	38.28581240023956	18.744864055311055	14.084373703610384
7	25.519348562284634	32.743989953091095	23.273319852377952	18.46334163224632
8	23.815604284008586	35.26615698485861	22.047408464112888	18.870830267019915
9	23.917631375768185	18.13040581583313	22.689034863598064	35.26292794480062
10-11	22.734893334881868	27.26700312990196	29.389823481762328	20.608280053453846
12-13	23.741786380847298	25.285075709739758	28.911851207702483	22.06128670171046
14-15	21.59371159421142	27.686126579598845	26.361439265196196	24.358722560993538
16-17	20.785340267954563	30.564782148358518	26.606458677033512	22.043418906653407
18-19	20.506176227898965	29.23245482181207	28.890769256325623	21.37059969396334
20-21	22.074071656516107	29.06784959738104	27.927993885407847	20.930084860695
22-23	21.666179807144825	29.25245574932091	27.87369016534455	21.207674278189717
24-25	21.3856133739803	28.89117351643285	28.194215099594473	21.528998009992378
26-27	21.205438879636777	28.59810094623405	28.394033443726567	21.8024267304026
28-29	21.315216245574774	28.75750290678144	28.260075384467694	21.66720546317609
30-31	21.728384063263345	29.48083999872984	27.622158753456187	21.168617184550627
32-33	21.004931925085547	28.927281302023516	28.418285110784552	21.649501662106385
34-35	21.12036807952672	28.289615951210468	28.529500357445116	22.060515611817696
36-37	22.006549231328997	29.053257525919445	27.640490567563297	21.29970267518826
38-39	21.689747821324737	29.039192263727216	27.883691269494136	21.38736864545391
40-41	21.253128594897955	28.732045264933642	28.2229139944209	21.7919121457475
42-43	21.10424824850488	28.827570675022056	28.084796795989224	21.983384280483836
44-45	21.615733525012907	29.082209570677847	27.79682631910679	21.505230585202455
46-47	21.503431886686432	29.551700888500363	27.446257592477753	21.498609632335448
48-49	21.286166391201423	28.908149076439184	28.017520589324103	21.788163943035297
50-51	21.3129375437994	28.594527836756846	28.08567195981253	22.006862659631224
52-53	21.324813044110403	28.80625343910778	27.815084751817515	22.053848764964297
54-55	21.63201203834134	29.40546576684887	27.580221061628578	21.38230113318121
56-57	21.137977579894798	29.10873414339993	27.627405238983467	22.125883037721806
58-59	20.97166343028751	28.484272079284185	28.25109108361618	22.292973406812123
60-61	21.66374911870843	29.211177158568972	27.449564249462995	21.6755094732596
62-63	21.43939911834819	29.096023892955092	27.55671305739999	21.907863931296728
64-65	21.4236995073783	28.59615680087747	27.966356753387007	22.013786938357228
66-67	21.270692119143796	28.78329802840771	27.597940479850024	22.348069372598466
68-69	21.90820876783508	29.08952073817811	27.435282688556555	21.566987805430248
70-71	22.039319495602903	28.645445846593038	27.619834302460646	21.695400355343413
72-73	21.455090188357126	28.83611839120308	28.14656552656163	21.562225893878164
74-75	21.438817887357676	28.814377916662316	27.886382913094803	21.860421282885202
76-77	21.64623188003124	28.941305906425864	27.408487422988248	22.003974790554643
78-79	21.783648900115228	29.0892377207686	27.615138972970442	21.511974406145722
80-81	21.44732303767498	28.768078930832313	27.867230878388966	21.917367153103736
82-83	21.285973150553954	28.868402784617857	27.929195530992356	21.916428533835834
84-85	21.479572417887084	28.81474935716114	27.75530563494515	21.95037259000663
86-87	21.745374225509153	29.02494131041264	27.60658511283926	21.62309935123895
88-89	21.25842561837853	28.93259843431873	27.77345798448491	22.035517962817828
90-91	21.1067424716437	28.8006526300438	27.758020563315345	22.334584334997153
92-93	21.45512160780115	29.1338765565456	27.46435382491952	21.94664801073373
94-95	21.36518330784318	28.81184620991586	27.78555574978025	22.037414732460714
96-97	21.224260935427115	28.908903587591617	27.83594271896729	22.03089275801398
98-99	21.198980941908395	28.932546939591553	27.811700645070243	22.05677147342981
100	21.537851812716223	29.052499987504692	27.502376313367012	21.907271886412076
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5140.0
1	3773.5
2	2908.0
3	3296.5
4	2862.5
5	2352.5
6	2078.0
7	1938.0
8	1889.5
9	1854.0
10	1656.0
11	1395.5
12	1345.0
13	1434.0
14	1494.5
15	1619.5
16	1801.0
17	2024.0
18	2460.0
19	3180.0
20	4163.0
21	5587.5
22	8020.0
23	11735.5
24	16351.5
25	21834.0
26	28911.5
27	37007.5
28	46746.0
29	57585.0
30	66850.5
31	74735.5
32	81880.0
33	88422.0
34	94830.0
35	99639.5
36	103257.0
37	107284.5
38	110903.0
39	111664.5
40	111958.0
41	112142.5
42	110075.5
43	108304.5
44	106808.5
45	105451.5
46	105057.0
47	104614.0
48	104291.0
49	104902.0
50	103493.0
51	99201.0
52	92864.0
53	87698.5
54	84831.0
55	81379.5
56	76667.5
57	70854.0
58	66023.5
59	61421.5
60	54630.0
61	46642.0
62	38967.0
63	31649.5
64	25250.0
65	20150.0
66	15647.5
67	11830.5
68	8986.5
69	6832.0
70	5148.5
71	4031.5
72	3114.0
73	2364.0
74	1850.0
75	1379.5
76	979.5
77	725.0
78	530.5
79	356.5
80	258.5
81	191.0
82	142.5
83	106.5
84	70.0
85	52.5
86	41.0
87	36.0
88	28.5
89	22.5
90	21.5
91	17.0
92	10.5
93	8.5
94	9.0
95	8.0
96	6.5
97	3.5
98	3.0
99	6.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07864161384939086
2	0.01364101264527752
3	0.017933227831076048
4	0.011377310115781034
5	0.00970158226927065
6	0.006879303790937369
7	0.0194031645385413
8	0.0088196202447915
9	0.009084208852135244
10-11	0.01299424049399281
12-13	0.015419636061310473
14-15	0.009613386066822735
16-17	0.01199468353291644
18-19	0.008246344928880052
20-21	0.013596914544053562
22-23	0.013449920873307038
24-25	0.015140348086892073
26-27	0.005335870248098858
28-29	0.012009382899991091
30-31	0.010554145559600495
32-33	0.011788892393871304
34-35	0.008393338599626578
36-37	0.010127863914435573
38-39	0.018947484159227073
40-41	0.012332768975633446
42-43	0.00793765822031235
44-45	0.02035862339839371
46-47	0.01787443036277744
48-49	0.01746284808468717
50-51	0.010216060116883486
52-53	0.010892231002317503
54-55	0.01099512657184007
56-57	0.019858844917855528
58-59	0.0021167088587499597
60-61	0.007320284803176945
62-63	0.014831661378324371
64-65	0.024239256306101974
66-67	0.012450363912230668
68-69	0.014258386062412925
70-71	0.025077120229357167
72-73	0.0199911392215274
74-75	0.02000583858860205
76-77	0.009451693029001557
78-79	0.01111272150843729
80-81	0.019285569601944077
82-83	0.01158310125482617
84-85	0.011245015812109163
86-87	0.016551487326058715
88-89	0.014567072770980627
90-91	0.014669968340503195
92-93	0.007805363916640477
94-95	0.01530204112471325
96-97	0.013258829101336555
98-99	0.009613386066822735
100	0.006761708854340149
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3401507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.91580940614723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.1296903047639	55.911182742228604
2	12.142410445928949	16.736080880457013
3	3.142860268341683	6.4977827772956465
4	1.2619190725021192	3.4786469714615262
5	0.6582858070189476	2.26831496056448
6	0.39186601212615635	1.6203458042659904
7	0.24247536711980064	1.1697270330279632
8	0.19024436075483378	1.0488675285099553
9	0.1387515457401467	0.8605957572932643
>10	0.6638664244977607	7.840699352494626
>50	0.029453664860271893	1.364635875487434
>100	0.007920711134633412	0.9079740439278311
>500	1.280076056928216E-4	0.05851759342062271
>1k	8.53384034024089E-5	0.06851557392649696
>5k	4.266920170120441E-5	0.16811310563853338
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5717	0.1680725631315767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.9398734149305E-5	0.0	2.9398734149305E-5	0.0
4	0.0	2.9398734149305E-5	2.9398734149305E-5	2.9398734149305E-5	0.0
5	0.0	2.9398734149305E-5	2.9398734149305E-5	2.9398734149305E-5	0.0
6	0.0	2.9398734149305E-5	2.9398734149305E-5	2.9398734149305E-5	0.0
7	0.0	2.9398734149305E-5	2.9398734149305E-5	2.9398734149305E-5	0.0
8	0.0	2.9398734149305E-5	2.9398734149305E-5	2.9398734149305E-5	0.0
9	0.0	2.9398734149305E-5	2.9398734149305E-5	8.819620244791499E-5	0.0
10-11	0.0	4.40981012239575E-5	2.9398734149305E-5	8.819620244791499E-5	0.0
12-13	0.0	5.879746829861E-5	2.9398734149305E-5	8.819620244791499E-5	0.0
14-15	0.0	5.879746829861E-5	2.9398734149305E-5	8.819620244791499E-5	0.0
16-17	0.0	5.879746829861E-5	2.9398734149305E-5	8.819620244791499E-5	0.0
18-19	0.0	5.879746829861E-5	2.9398734149305E-5	1.1759493659722E-4	0.0
20-21	0.0	5.879746829861E-5	2.9398734149305E-5	1.7639240489583E-4	0.0
22-23	0.0	5.879746829861E-5	2.9398734149305E-5	2.7928797441839745E-4	0.0
24-25	0.0	5.879746829861E-5	2.9398734149305E-5	5.438765817621425E-4	0.0
26-27	0.0	5.879746829861E-5	2.9398734149305E-5	8.231645561805399E-4	0.0
28-29	0.0	5.879746829861E-5	2.9398734149305E-5	0.001675727846510385	0.0
30-31	0.0	5.879746829861E-5	2.9398734149305E-5	0.0037483386040363872	0.0
32-33	0.0	5.879746829861E-5	2.9398734149305E-5	0.008422737333775882	0.0
34-35	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.015757721504027482	0.0
36-37	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.0264588607343745	0.0
38-39	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.0403203638857718	0.0
40-41	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.055666503111709016	0.0
42-43	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.07126253157791532	0.0
44-45	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.08922515814314066	0.0
46-47	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.11074503154043193	0.0
48-49	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.1342787182269506	0.0
50-51	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.15734202516708035	0.0
52-53	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.18133139223291322	0.0
54-55	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.2080254428404822	0.0
56-57	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.23957028458268642	0.0
58-59	2.9398734149305E-5	5.879746829861E-5	2.9398734149305E-5	0.27340822758853645	0.0
60-61	2.9398734149305E-5	5.879746829861E-5	5.879746829861E-5	0.30934818008606185	0.0
62-63	2.9398734149305E-5	5.879746829861E-5	5.879746829861E-5	0.3457291135958268	0.0
64-65	2.9398734149305E-5	7.34968353732625E-5	5.879746829861E-5	0.3875488129232132	0.0
66-67	2.9398734149305E-5	8.819620244791499E-5	5.879746829861E-5	0.43817343312831636	0.0
68-69	2.9398734149305E-5	8.819620244791499E-5	5.879746829861E-5	0.48997400269939173	0.0
70-71	2.9398734149305E-5	8.819620244791499E-5	5.879746829861E-5	0.5471251418856407	0.0
72-73	2.9398734149305E-5	8.819620244791499E-5	5.879746829861E-5	0.6146981323278182	0.0
74-75	2.9398734149305E-5	1.0289556952256749E-4	5.879746829861E-5	0.6843584328945964	0.0
76-77	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	0.7573113916860967	0.0
78-79	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	0.8430087017313208	0.0
80-81	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	0.9354383218967357	0.0
82-83	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	1.034835442055536	0.0
84-85	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	1.1406414862588847	0.0
86-87	2.9398734149305E-5	1.1759493659722E-4	5.879746829861E-5	1.2549584639984572	0.0
88	2.9398734149305E-5	1.46993670746525E-4	5.879746829861E-5	1.3499016759336377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3455	0.0	41.38382	1
GTATCAA	6210	0.0	37.79323	1
TCAACGC	6510	0.0	34.297474	4
ATCAACG	6485	0.0	34.141766	3
CAACGCA	7050	0.0	31.869986	5
TATCAAC	7435	0.0	30.285583	2
AACGCAG	7530	0.0	30.022612	6
ACGCAGA	8540	0.0	26.361862	7
CGCAGAG	8455	0.0	26.346613	8
ATAACGC	390	0.0	24.106848	3
TATAACG	395	0.0	23.802048	2
GTGGTAT	2020	0.0	23.749464	1
GTACCTG	2450	0.0	21.884861	1
GCAGAGT	9615	0.0	21.751219	9
TACCTGG	2355	0.0	21.159061	2
GTACATG	8250	0.0	20.580612	1
TGGTATC	2175	0.0	20.316553	2
TACATGG	8390	0.0	19.946629	2
TCTGTCG	1185	0.0	19.03632	8
AGAGTAC	8560	0.0	18.777233	10-11
>>END_MODULE