FastQCFastQC Report
Wed 25 May 2016
SRR2049440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401507
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172750.5078631324292439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164100.48243322739009503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100500.29545727820051526No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86970.2556807908965056No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48850.14361281631935494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.12614996823466776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77000.059.8996851
GTATCAA139150.040.5870251
TATAACG4850.038.764922
ATAACGC5000.036.6619223
GTGGTAT28550.035.9368131
TGGTATC31150.032.1397252
ATCAACG177650.031.3790723
TATCAAC178800.031.1509632
TCAACGC178500.031.1506524
TAACGCA6300.030.5889054
CAACGCA184950.030.089715
AACGCAG194950.028.712916
GCGGTAT2104.9112714E-1126.8937341
GTATAAC12150.025.5656491
ACGCAGA219550.025.4742977
TCTGTCG12300.025.218988
CGCAGAG221700.025.2060558
GTACCTG23600.024.9279861
TACCTGG23900.024.779542
CTGTCGC14650.023.41939
Produced by FastQC (version 0.11.5)