##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049440_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3401507
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.964919666489	33.0	31.0	34.0	30.0	34.0
2	32.04503592084332	33.0	31.0	34.0	30.0	34.0
3	32.0768909192308	34.0	31.0	34.0	30.0	34.0
4	35.790628977097505	37.0	35.0	37.0	33.0	37.0
5	35.84965693147184	37.0	35.0	37.0	35.0	37.0
6	35.92405836589488	37.0	35.0	37.0	35.0	37.0
7	35.89694332541429	37.0	35.0	37.0	35.0	37.0
8	35.89738077857844	37.0	35.0	37.0	35.0	37.0
9	37.58269702223161	39.0	37.0	39.0	35.0	39.0
10-11	37.570893283477	39.0	37.0	39.0	35.0	39.0
12-13	37.55544204377648	39.0	37.0	39.0	35.0	39.0
14-15	38.827566134657374	40.0	38.0	41.0	35.0	41.0
16-17	38.83181924952675	40.0	38.0	41.0	35.0	41.0
18-19	38.928777891681534	40.0	38.5	41.0	35.0	41.0
20-21	38.94649930163307	40.0	38.5	41.0	35.0	41.0
22-23	38.88597039488674	40.0	38.0	41.0	35.0	41.0
24-25	38.848376028624955	40.0	38.0	41.0	35.0	41.0
26-27	38.72448858697042	40.0	38.0	41.0	35.0	41.0
28-29	38.6210506108028	40.0	38.0	41.0	35.0	41.0
30-31	38.46085470351818	40.0	38.0	41.0	34.0	41.0
32-33	38.24955233077574	40.0	38.0	41.0	34.0	41.0
34-35	38.0818534255552	40.0	38.0	41.0	34.0	41.0
36-37	37.907045024455336	40.0	37.5	41.0	33.0	41.0
38-39	37.76461006841967	40.0	37.0	41.0	33.0	41.0
40-41	37.644907536571296	40.0	37.0	41.0	33.0	41.0
42-43	37.470470882464745	40.0	37.0	41.0	33.0	41.0
44-45	37.25019557507893	40.0	37.0	41.0	32.5	41.0
46-47	36.93338967110754	40.0	37.0	41.0	31.0	41.0
48-49	36.62246395494703	40.0	36.5	41.0	30.5	41.0
50-51	36.442378628061036	40.0	36.0	41.0	30.0	41.0
52-53	36.311365227236045	40.0	36.0	41.0	30.0	41.0
54-55	36.15552356646627	39.5	35.5	41.0	30.0	41.0
56-57	36.0078412303723	39.0	35.0	41.0	29.5	41.0
58-59	35.82702122911992	39.0	35.0	41.0	29.0	41.0
60-61	35.62825653453014	39.0	35.0	41.0	29.0	41.0
62-63	35.38732141371457	38.5	35.0	40.5	28.0	41.0
64-65	35.15276199637396	38.0	35.0	40.0	28.0	41.0
66-67	34.885931882545	37.0	35.0	40.0	28.0	41.0
68-69	34.581605447232654	37.0	34.0	40.0	28.0	41.0
70-71	34.287163895297	36.5	34.0	39.0	27.5	41.0
72-73	33.95290910763964	36.0	34.0	39.0	26.5	41.0
74-75	33.64142437454928	35.5	34.0	39.0	26.0	40.5
76-77	33.33548541866884	35.0	34.0	37.5	26.0	40.0
78-79	33.03800462559683	35.0	34.0	37.0	26.0	39.0
80-81	32.74794392603043	35.0	34.0	37.0	26.0	39.0
82-83	32.49022786076877	35.0	33.5	36.0	26.0	39.0
84-85	32.217005727167404	35.0	33.0	36.0	25.5	37.0
86-87	31.975955216320298	35.0	33.0	36.0	25.0	37.0
88-89	31.761747807662896	35.0	33.0	35.0	25.0	37.0
90-91	31.577384376983495	35.0	33.0	35.0	25.0	36.0
92-93	31.410798213850505	35.0	33.0	35.0	24.0	36.0
94-95	31.240130918442915	35.0	33.0	35.0	23.5	36.0
96-97	31.08818929374539	35.0	33.0	35.0	23.0	36.0
98-99	30.954274237859867	35.0	33.0	35.0	20.0	35.0
100	30.846598875145634	35.0	33.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2891310953751436
1101	2	0.3170253630960147
1101	3	0.4457131994214336
1101	4	0.299518867230546
1101	5	0.2732720739573651
1101	6	0.2774665409699324
1101	7	0.24156843815531914
1101	8	0.27069063154524997
1101	9	0.3285843759423841
1101	10-11	0.3110697317600568
1101	12-13	0.27676890316105585
1101	14-15	0.4094874329184677
1101	16-17	0.41969787598282693
1101	18-19	0.36870273801251585
1101	20-21	0.3843816158752631
1101	22-23	0.3887508962200883
1101	24-25	0.3853091857910087
1101	26-27	0.3705668573233041
1101	28-29	0.37977438166716126
1101	30-31	0.37006448347091947
1101	32-33	0.363882469469921
1101	34-35	0.3536915409801935
1101	36-37	0.36233491194866474
1101	38-39	0.35451608225510967
1101	40-41	0.40350690855436255
1101	42-43	0.42303007561761774
1101	44-45	0.3540939386299229
1101	46-47	0.4007406801343407
1101	48-49	0.3786752434255547
1101	50-51	0.383900224323952
1101	52-53	0.45125891556047293
1101	54-55	0.4500533314806674
1101	56-57	0.4952745884623866
1101	58-59	0.4981819541984862
1101	60-61	0.3761072270997161
1101	62-63	0.38183635148342887
1101	64-65	0.40686550484380746
1101	66-67	0.3815912124303509
1101	68-69	0.37898754301445337
1101	70-71	0.44810956915373623
1101	72-73	0.4844267459934244
1101	74-75	0.4512710046419386
1101	76-77	0.3947210245572066
1101	78-79	0.3880990978265828
1101	80-81	0.3577769365651662
1101	82-83	0.2582344347084842
1101	84-85	0.302020346915441
1101	86-87	0.32106479629985074
1101	88-89	0.40250235268965895
1101	90-91	0.4405429389921238
1101	92-93	0.3470923339793437
1101	94-95	0.3701454741535244
1101	96-97	0.3260974714663618
1101	98-99	0.40928853429649337
1101	100	0.38225928543634424
1102	1	0.29167353913959815
1102	2	0.28177858342971973
1102	3	0.3686989735686481
1102	4	0.2686625253577688
1102	5	0.26580307553831517
1102	6	0.2310865829558324
1102	7	0.2474126823223486
1102	8	0.2551275050442783
1102	9	0.23585826646005614
1102	10-11	0.25771178209875245
1102	12-13	0.24656025640419443
1102	14-15	0.3042930779621429
1102	16-17	0.3267848521067833
1102	18-19	0.3337472900022931
1102	20-21	0.31582148287731826
1102	22-23	0.29628799484490287
1102	24-25	0.27297434294544587
1102	26-27	0.2325112907695157
1102	28-29	0.30578582085303907
1102	30-31	0.30231121113674675
1102	32-33	0.3610237554767508
1102	34-35	0.40088104503025335
1102	36-37	0.41144322891267393
1102	38-39	0.37290390803499207
1102	40-41	0.45048095832500934
1102	42-43	0.39723597255336074
1102	44-45	0.37697657783201777
1102	46-47	0.4315023886588776
1102	48-49	0.44910177253787964
1102	50-51	0.4051743672249657
1102	52-53	0.40991498795511205
1102	54-55	0.38329878861156885
1102	56-57	0.3963223915427463
1102	58-59	0.4086224813857555
1102	60-61	0.28308753774273754
1102	62-63	0.2995233242683639
1102	64-65	0.28024231705889235
1102	66-67	0.2854566162476715
1102	68-69	0.3268016021826554
1102	70-71	0.32471307461775467
1102	72-73	0.40321128659306993
1102	74-75	0.3219696473049538
1102	76-77	0.2756374875415162
1102	78-79	0.2758265181452444
1102	80-81	0.3033130090863736
1102	82-83	0.29541237167401135
1102	84-85	0.24372362311528661
1102	86-87	0.215864737529742
1102	88-89	0.25379687069595747
1102	90-91	0.22769176360888466
1102	92-93	0.29202242832916525
1102	94-95	0.245832226862845
1102	96-97	0.2221394910044623
1102	98-99	0.27704019957072035
1102	100	0.27099482361428073
1103	1	0.2652459765386048
1103	2	0.31956570609829527
1103	3	0.3999267765519292
1103	4	0.20226182948550786
1103	5	0.22656624582357665
1103	6	0.1929951188008161
1103	7	0.25573603158873937
1103	8	0.188141147004508
1103	9	0.2767809677068698
1103	10-11	0.26405445668730465
1103	12-13	0.25233024821289973
1103	14-15	0.3524533272372139
1103	16-17	0.3046602080488796
1103	18-19	0.3300425099373143
1103	20-21	0.35793871037225955
1103	22-23	0.3734267695319531
1103	24-25	0.33996196873935247
1103	26-27	0.38555839419478133
1103	28-29	0.3843233106974253
1103	30-31	0.42513934202967363
1103	32-33	0.3846577513947409
1103	34-35	0.36029562675449966
1103	36-37	0.42346656895062296
1103	38-39	0.3638199465294818
1103	40-41	0.38944328505873216
1103	42-43	0.36829541939641075
1103	44-45	0.3539680877831515
1103	46-47	0.26482982592929716
1103	48-49	0.2489186645689685
1103	50-51	0.2027021983523909
1103	52-53	0.2967553135964778
1103	54-55	0.217837615272515
1103	56-57	0.19965742205678083
1103	58-59	0.273659850552022
1103	60-61	0.25133393875977816
1103	62-63	0.2673426531241532
1103	64-65	0.23956197820712788
1103	66-67	0.27862706369927537
1103	68-69	0.258738880630041
1103	70-71	0.19078682273368486
1103	72-73	0.25640988462602365
1103	74-75	0.2671846783293148
1103	76-77	0.2265225992743254
1103	78-79	0.20923088998131334
1103	80-81	0.1582415079539814
1103	82-83	0.18805989670199352
1103	84-85	0.23526104224715993
1103	86-87	0.21243689131404153
1103	88-89	0.20870985869225933
1103	90-91	0.17870395260212746
1103	92-93	0.23931239054566333
1103	94-95	0.1773821359890242
1103	96-97	0.18451313163495797
1103	98-99	0.21705363534985977
1103	100	0.1860149694349822
1104	1	0.14735973388421897
1104	2	0.1957864955850468
1104	3	0.21241059194473166
1104	4	0.20979344173869663
1104	5	0.2131317255205616
1104	6	0.15424226681718523
1104	7	0.17672763686265824
1104	8	0.18980933802946254
1104	9	0.22073973642088873
1104	10-11	0.24355056257836338
1104	12-13	0.2195299073974013
1104	14-15	0.34837865120037037
1104	16-17	0.2559300654881511
1104	18-19	0.2465319131262902
1104	20-21	0.2383439093425963
1104	22-23	0.22636704475549152
1104	24-25	0.25230572527605943
1104	26-27	0.2325385270012177
1104	28-29	0.2371104130570103
1104	30-31	0.26169092306987096
1104	32-33	0.30873015057997577
1104	34-35	0.32416692648416756
1104	36-37	0.30432389007150107
1104	38-39	0.2755000192134176
1104	40-41	0.28319182069054705
1104	42-43	0.33484957275453553
1104	44-45	0.2715352189998086
1104	46-47	0.3060840643543301
1104	48-49	0.36910003470219266
1104	50-51	0.3485896850177639
1104	52-53	0.4057874681183904
1104	54-55	0.35617564430249615
1104	56-57	0.39824877959296856
1104	58-59	0.30400455328532416
1104	60-61	0.3061764378036216
1104	62-63	0.2938459807987499
1104	64-65	0.3386686368084142
1104	66-67	0.3827322686454693
1104	68-69	0.3121527120300769
1104	70-71	0.28051741577291267
1104	72-73	0.35109896973671795
1104	74-75	0.3675479101264685
1104	76-77	0.3398542581681383
1104	78-79	0.32203667755184284
1104	80-81	0.29157365289965753
1104	82-83	0.29307862085187253
1104	84-85	0.29073489995583657
1104	86-87	0.2660522072542051
1104	88-89	0.3175462227598089
1104	90-91	0.33776092042357675
1104	92-93	0.2543642855824153
1104	94-95	0.2749412947029164
1104	96-97	0.26638527972470527
1104	98-99	0.3294932795082417
1104	100	0.28382194806737004
1105	1	0.19788772867284976
1105	2	0.17830727455836382
1105	3	0.2548138718162818
1105	4	0.16537596476658933
1105	5	0.17542757593719216
1105	6	0.12157852471335673
1105	7	0.1276288909100387
1105	8	0.10853082639119549
1105	9	0.20493320582194485
1105	10-11	0.14987810102014265
1105	12-13	0.11688533235080456
1105	14-15	0.2001581384791038
1105	16-17	0.2220179059458971
1105	18-19	0.21787361707668396
1105	20-21	0.13130776654667642
1105	22-23	0.1411443777386836
1105	24-25	0.21137149015194723
1105	26-27	0.18563697049725647
1105	28-29	0.23369741166199276
1105	30-31	0.2718729584802375
1105	32-33	0.22661192799962748
1105	34-35	0.23770896071959413
1105	36-37	0.19519560296937755
1105	38-39	0.19303002066450148
1105	40-41	0.17859623244118694
1105	42-43	0.20188382156339912
1105	44-45	0.26771020275061375
1105	46-47	0.18292013602628998
1105	48-49	0.20275130823637966
1105	50-51	0.12499994234986644
1105	52-53	0.12229696165202597
1105	54-55	0.13903180661809955
1105	56-57	0.12084899816641581
1105	58-59	0.14331879840106865
1105	60-61	0.11533580434603863
1105	62-63	0.1378448414997564
1105	64-65	0.067402110987274
1105	66-67	0.04471501454576554
1105	68-69	0.14082081458779783
1105	70-71	0.11962338733204803
1105	72-73	0.16719714833464394
1105	74-75	0.14501816148747082
1105	76-77	0.17525305221242604
1105	78-79	0.17733222252864778
1105	80-81	0.18031675306120576
1105	82-83	0.10051398873476103
1105	84-85	0.1577119313850801
1105	86-87	0.20277642124383988
1105	88-89	0.1556640279898609
1105	90-91	0.15509910213939904
1105	92-93	0.17735850853942026
1105	94-95	0.20005982761870555
1105	96-97	0.22001388278456702
1105	98-99	0.15102525419398916
1105	100	0.07936841649672388
1106	1	0.1521204567989365
1106	2	0.15573157302482343
1106	3	0.2982304635840052
1106	4	0.17155140458566365
1106	5	0.12373934194993552
1106	6	0.1297258317168115
1106	7	0.10101236930609048
1106	8	0.15124161106404443
1106	9	0.15205395958818713
1106	10-11	0.13737079725684964
1106	12-13	0.12996532263031924
1106	14-15	0.20638130217452755
1106	16-17	0.22153132342811688
1106	18-19	0.19637698886906207
1106	20-21	0.16390172227679756
1106	22-23	0.12085216317809255
1106	24-25	0.23247605294167784
1106	26-27	0.24570941846855732
1106	28-29	0.23209114212944826
1106	30-31	0.20005742161119855
1106	32-33	0.20932680158868777
1106	34-35	0.23532116617914056
1106	36-37	0.16477446097945858
1106	38-39	0.18831488679046515
1106	40-41	0.17813507535831263
1106	42-43	0.19733125412307828
1106	44-45	0.2356863277088408
1106	46-47	0.19453386418275187
1106	48-49	0.29463332051921753
1106	50-51	0.33704985631287343
1106	52-53	0.328096015691834
1106	54-55	0.29066735076558814
1106	56-57	0.33563745110279086
1106	58-59	0.2771194236806309
1106	60-61	0.18976956539250978
1106	62-63	0.2167931993516916
1106	64-65	0.21376077922160874
1106	66-67	0.2555715337910698
1106	68-69	0.27148394864528314
1106	70-71	0.3044684582075874
1106	72-73	0.3133585926244322
1106	74-75	0.2555424525682639
1106	76-77	0.24763267636568287
1106	78-79	0.3139029172989396
1106	80-81	0.21182821066304314
1106	82-83	0.2282334307123577
1106	84-85	0.22347900670511933
1106	86-87	0.2392884253250145
1106	88-89	0.22102254672412514
1106	90-91	0.2950282995622793
1106	92-93	0.3108251411367462
1106	94-95	0.25314861964028523
1106	96-97	0.30669916697466704
1106	98-99	0.38754508320403147
1106	100	0.2973331130383876
1107	1	0.12178185571232447
1107	2	0.15269894390507943
1107	3	0.23038505908544948
1107	4	0.1324792668766932
1107	5	0.12715830375147164
1107	6	0.1320222855015274
1107	7	0.09086352516072083
1107	8	0.08850920695148545
1107	9	0.12949037241614292
1107	10-11	0.13795496744283042
1107	12-13	0.1369693198271804
1107	14-15	0.20690513192172943
1107	16-17	0.1916731927023676
1107	18-19	0.1971954316329132
1107	20-21	0.16287500058491844
1107	22-23	0.1985204745338791
1107	24-25	0.18921443130722082
1107	26-27	0.1549621246657935
1107	28-29	0.18692071830876245
1107	30-31	0.22332393642659554
1107	32-33	0.2716653991581808
1107	34-35	0.26322257407874616
1107	36-37	0.15874849546649017
1107	38-39	0.18105260174090887
1107	40-41	0.028390769341498867
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2216	64-65	-0.6393732983569365
2216	66-67	-0.5480389481272283
2216	68-69	-0.5792760108880088
2216	70-71	-0.6265577521672085
2216	72-73	-0.5885991423558394
2216	74-75	-0.5937955467101759
2216	76-77	-0.5495075028333929
2216	78-79	-0.5676994229263812
2216	80-81	-0.5795701967042746
2216	82-83	-0.531078467912593
2216	84-85	-0.7315882580356572
2216	86-87	-0.6686161115580838
2216	88-89	-0.6344584510330975
2216	90-91	-0.6595194161888642
2216	92-93	-0.572811692492273
2216	94-95	-0.615915504095593
2216	96-97	-0.6925107001323738
2216	98-99	-0.7918483253348896
2216	100	-0.8558722368981968
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	230.0
3	21.0
4	44.0
5	127.0
6	452.0
7	1171.0
8	2528.0
9	4053.0
10	4787.0
11	6377.0
12	9743.0
13	13941.0
14	14294.0
15	16604.0
16	20609.0
17	28000.0
18	28268.0
19	14904.0
20	10542.0
21	9581.0
22	9876.0
23	10379.0
24	12029.0
25	14279.0
26	18116.0
27	22955.0
28	29416.0
29	38241.0
30	49147.0
31	63894.0
32	82920.0
33	109163.0
34	151507.0
35	226870.0
36	358533.0
37	687405.0
38	950447.0
39	378940.0
40	1114.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.017509613097836	19.836094621610105	13.681239565697547	22.465156199594517
2	16.748929224605448	21.171998176102534	38.9503240769459	23.128748522346125
3	17.838534508381137	26.207354563727197	31.19546718557392	24.758643742317744
4	12.818465462514114	18.373385678759444	38.104052115724	30.70409674300244
5	11.935661326377991	36.45826809760412	35.11386078790942	16.49220978810847
6	28.40615269914823	38.37887354957329	18.41873244707701	14.796241304201471
7	24.726804913234048	33.04955715216814	23.59248415481726	18.63115377978055
8	24.085177540425466	34.243410347237266	22.15606200428222	19.515350108055046
9	24.071654122716783	16.6705521993634	23.245490895653013	36.0123027822668
10-11	22.774640769517745	26.552583898842485	29.54048014600587	21.132295185633897
12-13	24.394716812283495	24.583486084256183	29.573186237746974	21.448610865713345
14-15	21.872467115310947	26.417129231249557	28.141306191637998	23.569097461801487
16-17	20.005412306956888	29.396044165130338	28.40984892872483	22.188694599187947
18-19	19.60689776619598	26.905368708634143	32.48505735840026	21.00267616676961
20-21	21.129355312218966	26.647497712043517	32.04732490628419	20.175822069453332
22-23	20.707527575277666	26.86350491120553	32.047956979068395	20.381010534448407
24-25	20.46822187930232	26.535223946327317	32.30066849781582	20.69588567655454
26-27	20.29637157883256	26.244485164957766	32.52327571279436	20.935867543415316
28-29	20.455374632479074	26.436091414775863	32.349308703465844	20.75922524927922
30-31	20.882303049795283	27.065415417342958	31.730362454053452	20.321919078808303
32-33	20.147952069479793	26.625022379786373	32.457981124248754	20.769044426485085
34-35	20.26337149974996	26.06310967462363	32.401035776201546	21.27248304942486
36-37	21.238365818444592	26.903957569394976	31.322366821529396	20.535309790631036
38-39	20.987388825011973	26.92649169912042	31.530465761205257	20.555653714662352
40-41	20.483891404603902	26.69193389871019	31.71239982748823	21.11177486919768
42-43	20.45444857235337	26.90642706306352	31.341358403789847	21.29776596079326
44-45	21.03783705281218	27.140411588157836	30.870390682717986	20.951360676312
46-47	21.013039220557243	27.78040733122113	30.135760414428077	21.07079303379355
48-49	20.972189679456783	27.317053882293933	30.34575557245656	21.365000865792723
50-51	21.067147590759035	27.099532648323226	30.167893230853267	21.66542653006447
52-53	21.078671894545565	27.390844705008693	29.855105398871736	21.675378001574007
54-55	21.347964299353198	28.066897995506107	29.491340161875314	21.09379754326538
56-57	20.80070686316389	27.69573897687113	29.606774291512554	21.896779868452423
58-59	20.590976293742745	27.094387869847097	30.263497914306807	22.05113792210335
60-61	21.317595406976967	27.760666081239876	29.529102835890093	21.392635675893068
62-63	21.072160074931492	27.577129783945765	29.67521454461214	21.675495596510604
64-65	21.066383223671156	27.156316303332613	30.11685702837007	21.66044344462616
66-67	20.970146467433405	27.405382379045523	29.663587345256087	21.960883808264985
68-69	21.684712099666413	27.723256192034885	29.387592029062414	21.20443967923629
70-71	21.772673112241133	27.301031572182566	29.578933690273164	21.347361625303137
72-73	21.272865232968797	27.482098375808135	29.967614354461126	21.27742203676194
74-75	21.29062519798213	27.511618748927862	29.673881264081075	21.52387478900893
76-77	21.526370517538258	27.641307220593696	29.222327045042096	21.609995216825954
78-79	21.625164963646995	27.86000440393038	29.34988815251593	21.1649424799067
80-81	21.297997751286527	27.507316256457997	29.633304478420623	21.561381513834853
82-83	21.120375200776248	27.604659787613382	29.686573704341825	21.588391307268544
84-85	21.29864956438272	27.642448394014984	29.438673289244875	21.62022875235742
86-87	21.580600280612114	27.770133257680268	29.27323814472618	21.376028316981444
88-89	21.08923474396066	27.65948971171155	29.440841937163402	21.81043360716439
90-91	20.87250127517834	27.553789006860242	29.429484475163054	22.144225242798367
92-93	21.245027376896573	27.87203114391168	29.078366761482695	21.80457471770906
94-95	21.098651564296546	27.55166328237244	29.40217204366195	21.947513109669067
96-97	20.950744830082076	27.688516809077697	29.430287782040793	21.930450578799437
98-99	20.92072639845196	27.7228072068327	29.403435253588416	21.95303114112692
100	21.272455521119788	27.792575162384146	29.076772555182973	21.85819676131309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2199.0
1	1668.5
2	4280.0
3	6184.0
4	3122.5
5	1362.5
6	2456.0
7	5558.5
8	8751.0
9	9804.5
10	7201.0
11	4012.5
12	3439.5
13	3749.0
14	4202.5
15	4647.5
16	5114.0
17	5697.5
18	6405.5
19	7128.0
20	8136.0
21	9549.5
22	11643.5
23	14950.0
24	19004.0
25	23946.0
26	30573.0
27	38326.5
28	47619.5
29	57444.5
30	65964.5
31	73598.0
32	80814.5
33	87493.5
34	93159.5
35	97273.5
36	100737.0
37	104547.5
38	107514.0
39	108309.0
40	109213.5
41	109478.0
42	107558.5
43	105862.5
44	104782.5
45	103729.0
46	103341.5
47	102529.5
48	102257.0
49	103251.5
50	102003.5
51	97699.5
52	91070.0
53	85822.5
54	82774.0
55	79010.5
56	73981.0
57	68475.0
58	63944.5
59	60025.5
60	53936.0
61	45796.0
62	37885.5
63	30653.5
64	24320.0
65	19026.5
66	14545.0
67	10820.0
68	8129.5
69	6150.0
70	4475.0
71	3300.5
72	2532.5
73	1934.0
74	1472.5
75	1104.0
76	753.5
77	519.0
78	358.5
79	207.5
80	119.5
81	70.5
82	36.5
83	19.5
84	14.5
85	9.0
86	4.0
87	4.0
88	3.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11932946191202899
2	0.0
3	0.0
4	0.0
5	0.011171518976735899
6	2.3518987319443997E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.4699367074652498E-5
76-77	0.0
78-79	0.0
80-81	1.9109177197048247E-4
82-83	6.320727842100575E-4
84-85	3.5278480979165997E-4
86-87	4.262816451649225E-4
88-89	5.291772146874899E-4
90-91	6.4677215128471E-4
92-93	2.204905061197875E-4
94-95	2.9398734149305E-4
96-97	7.790664549565824E-4
98-99	0.0011906487330468525
100	1.46993670746525E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3401507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.45353279419608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7662632582727	52.209837280458295
2	12.9773871137674	16.988316660679043
3	3.4280833516656415	6.731404982384539
4	1.2994628789037534	3.4021774463666916
5	0.686961126060492	2.2482016296469136
6	0.43100028107315674	1.6926294618917754
7	0.2873564674108504	1.3165947174310304
8	0.20697478838605896	1.0837784879358963
9	0.153807185763457	0.9060501309616298
>10	0.7142156427773894	8.051809603663559
>50	0.03447683384531917	1.52609053626817
>100	0.012933432556255854	1.4492580874018486
>500	4.93918114056708E-4	0.22950427676861873
>1k	4.041148177814468E-4	0.6233852207852942
>5k	4.490164642016069E-5	0.2556023414817642
>10k+	1.3470493926048206E-4	1.2853591358750094
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17275	0.5078631324292439	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16410	0.48243322739009503	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10050	0.29545727820051526	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8697	0.2556807908965056	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4885	0.14361281631935494	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4291	0.12614996823466776	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.819620244791499E-5	0.0
10-11	0.0	0.0	0.0	8.819620244791499E-5	0.0
12-13	0.0	0.0	0.0	8.819620244791499E-5	0.0
14-15	0.0	0.0	0.0	1.0289556952256749E-4	0.0
16-17	0.0	0.0	0.0	1.1759493659722E-4	0.0
18-19	0.0	0.0	0.0	1.46993670746525E-4	0.0
20-21	0.0	0.0	0.0	1.910917719704825E-4	0.0
22-23	0.0	0.0	0.0	2.9398734149305E-4	0.0
24-25	0.0	0.0	0.0	5.58575948836795E-4	0.0
26-27	0.0	0.0	0.0	8.084651891058874E-4	0.0
28-29	0.0	0.0	0.0	0.0016463291123610798	0.0
30-31	0.0	0.0	0.0	0.00376303797111104	0.0
32-33	0.0	0.0	0.0	0.008510933536223798	0.0
34-35	0.0	0.0	0.0	0.015919414541848656	0.0
36-37	0.0	0.0	0.0	0.02681164554416616	0.0
38-39	0.0	0.0	0.0	0.04089363920168325	0.0
40-41	0.0	0.0	0.0	0.05625447779469511	0.0
42-43	0.0	0.0	0.0	0.07193870246334932	0.0
44-45	0.0	0.0	0.0	0.09022471510421703	0.0
46-47	0.0	0.0	0.0	0.11206797457715066	0.0
48-49	0.0	0.0	0.0	0.1358221517697891	0.0
50-51	0.0	0.0	0.0	0.15900305364651607	0.0
52-53	0.0	0.0	0.0	0.18312471501602084	0.0
54-55	0.0	2.9398734149305E-5	0.0	0.20992166119311234	0.0
56-57	0.0	2.9398734149305E-5	0.0	0.24181928774510827	0.0
58-59	0.0	2.9398734149305E-5	0.0	0.2761570092314965	0.0
60-61	0.0	4.40981012239575E-5	0.0	0.31214105983024587	0.0
62-63	0.0	5.879746829861E-5	0.0	0.3484484965046375	0.0
64-65	0.0	5.879746829861E-5	0.0	0.3905033857052183	0.0
66-67	0.0	8.819620244791499E-5	0.0	0.4411133065432469	0.0
68-69	0.0	8.819620244791499E-5	0.0	0.49288447738017294	0.0
70-71	0.0	8.819620244791499E-5	0.0	0.5501826102371683	0.0
72-73	0.0	8.819620244791499E-5	0.0	0.617667404476898	0.0
74-75	0.0	8.819620244791499E-5	0.0	0.6875775942839453	0.0
76-77	0.0	8.819620244791499E-5	0.0	0.7613537176316263	0.0
78-79	0.0	8.819620244791499E-5	0.0	0.8474626099549406	0.0
80-81	0.0	1.0289556952256749E-4	0.0	0.9408035908789839	0.0
82-83	0.0	1.1759493659722E-4	0.0	1.0409356793915168	0.0
84-85	0.0	1.1759493659722E-4	0.0	1.1474766919485981	0.0
86-87	0.0	1.1759493659722E-4	0.0	1.2624404418394553	0.0
88	0.0	1.1759493659722E-4	0.0	1.358221517697891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7700	0.0	59.899685	1
GTATCAA	13915	0.0	40.587025	1
TATAACG	485	0.0	38.76492	2
ATAACGC	500	0.0	36.661922	3
GTGGTAT	2855	0.0	35.936813	1
TGGTATC	3115	0.0	32.139725	2
ATCAACG	17765	0.0	31.379072	3
TATCAAC	17880	0.0	31.150963	2
TCAACGC	17850	0.0	31.150652	4
TAACGCA	630	0.0	30.588905	4
CAACGCA	18495	0.0	30.08971	5
AACGCAG	19495	0.0	28.71291	6
GCGGTAT	210	4.9112714E-11	26.893734	1
GTATAAC	1215	0.0	25.565649	1
ACGCAGA	21955	0.0	25.474297	7
TCTGTCG	1230	0.0	25.21898	8
CGCAGAG	22170	0.0	25.206055	8
GTACCTG	2360	0.0	24.927986	1
TACCTGG	2390	0.0	24.77954	2
CTGTCGC	1465	0.0	23.4193	9
>>END_MODULE