FastQCFastQC Report
Wed 25 May 2016
SRR2049439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6536585
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67850.044.9881551
GTATCAA115250.034.4841161
TCAACGC124100.031.2053934
ATCAACG124750.031.0082113
TATCAAC140800.027.873912
CAACGCA140050.027.7198625
AACGCAG146600.026.4180456
GTACATG197300.024.434311
ACGCAGA160600.023.8231727
TACATGG199400.023.2887342
CGCAGAG163900.023.1687858
ACATGGG204750.021.9228423
GTGGTAT39400.020.8903261
GCAGAGT187900.019.6346789
CATGGGG143700.018.2495274
TGGTATC41750.018.0126482
TATACCG4700.017.9998465
AGAGTAC166650.017.35855510-11
CAGAGTA181450.017.35436210-11
GAGTACA148350.016.1056961
Produced by FastQC (version 0.11.5)