##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049439_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6536585
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.990733234555965	31.0	31.0	34.0	28.0	34.0
2	31.230378859909266	31.0	31.0	34.0	28.0	34.0
3	31.31529093555733	33.0	31.0	34.0	28.0	34.0
4	34.90943650239383	37.0	35.0	37.0	32.0	37.0
5	34.87850154170717	37.0	35.0	37.0	32.0	37.0
6	34.96418037859218	37.0	35.0	37.0	32.0	37.0
7	34.87913428801125	37.0	35.0	37.0	32.0	37.0
8	34.87889257157981	37.0	35.0	37.0	32.0	37.0
9	36.44661348395225	39.0	37.0	39.0	32.0	39.0
10-11	36.38520771932133	39.0	37.0	39.0	32.0	39.0
12-13	36.30278853560384	39.0	37.0	39.0	32.0	39.0
14-15	37.55390536495739	40.0	38.0	41.0	32.0	41.0
16-17	37.550132446835775	40.0	38.0	41.0	32.0	41.0
18-19	37.57483686053192	40.0	38.0	41.0	32.0	41.0
20-21	37.62884181877846	40.0	38.0	41.0	32.0	41.0
22-23	37.59444824782359	40.0	38.0	41.0	32.0	41.0
24-25	37.59924861376391	40.0	38.0	41.0	32.0	41.0
26-27	37.60116658775186	40.0	38.0	41.0	32.0	41.0
28-29	37.58235737774388	40.0	38.0	41.0	32.0	41.0
30-31	37.52949850724805	40.0	38.0	41.0	32.0	41.0
32-33	37.482240497140324	40.0	38.0	41.0	32.0	41.0
34-35	37.41162296520278	40.0	38.0	41.0	32.0	41.0
36-37	37.297611902851415	40.0	37.5	41.0	31.0	41.0
38-39	37.19044699946532	40.0	37.0	41.0	31.0	41.0
40-41	37.065778613756265	40.0	37.0	41.0	31.0	41.0
42-43	36.97011765317823	40.0	37.0	41.0	30.5	41.0
44-45	36.82056869144974	40.0	37.0	41.0	30.0	41.0
46-47	36.700088578363165	40.0	36.5	41.0	30.0	41.0
48-49	36.594688663881826	40.0	36.0	41.0	30.0	41.0
50-51	36.47746606217161	40.0	36.0	41.0	30.0	41.0
52-53	36.36010049590115	40.0	36.0	41.0	30.0	41.0
54-55	36.21029650803899	39.0	35.0	41.0	30.0	41.0
56-57	36.042532836335795	39.0	35.0	41.0	29.0	41.0
58-59	35.855330115037134	39.0	35.0	41.0	29.0	41.0
60-61	35.66111822916706	39.0	35.0	41.0	29.0	41.0
62-63	35.41020464049653	38.5	35.0	40.0	28.0	41.0
64-65	35.17391137727116	38.0	34.5	40.0	28.0	41.0
66-67	34.95016503265849	37.5	34.0	40.0	28.0	41.0
68-69	34.666778524259996	37.0	34.0	40.0	28.0	41.0
70-71	34.36046131121985	36.5	34.0	39.5	27.5	41.0
72-73	34.02933917328391	36.0	34.0	39.0	27.0	41.0
74-75	33.72830660046492	36.0	34.0	39.0	26.5	40.5
76-77	33.40181524450458	35.0	34.0	38.5	26.0	40.0
78-79	33.05781658159421	35.0	33.0	37.0	26.0	39.0
80-81	32.72629392871048	35.0	33.0	37.0	26.0	39.0
82-83	32.425366456643644	35.0	33.0	36.5	26.0	39.0
84-85	32.13808785474372	35.0	33.0	36.0	25.0	37.5
86-87	31.867851714618567	35.0	33.0	36.0	25.0	37.0
88-89	31.63886134732433	35.0	33.0	35.0	25.0	37.0
90-91	31.424786031238025	35.0	33.0	35.0	24.0	36.0
92-93	31.21614053821682	35.0	32.0	35.0	23.5	36.0
94-95	31.00968380278081	35.0	32.0	35.0	22.5	36.0
96-97	30.818735547690423	35.0	32.0	35.0	20.0	36.0
98-99	30.640037190673723	35.0	32.0	35.0	18.5	35.0
100	30.499861319022088	35.0	32.0	35.0	17.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2511656917822336
1101	2	0.2372191700897801
1101	3	0.2674672600616894
1101	4	0.3340101337178396
1101	5	0.27462993343608133
1101	6	0.2942174769843078
1101	7	0.3779082851291733
1101	8	0.37814594429260495
1101	9	0.4412286043683551
1101	10-11	0.5166305209241386
1101	12-13	0.5301504274559576
1101	14-15	0.6114345821553187
1101	16-17	0.5124021889497428
1101	18-19	0.5373623488975596
1101	20-21	0.5485686628747857
1101	22-23	0.5007545588617788
1101	24-25	0.5503715195352825
1101	26-27	0.5320488449951029
1101	28-29	0.5130037141738413
1101	30-31	0.546441041989354
1101	32-33	0.5369867613867356
1101	34-35	0.5282777076005374
1101	36-37	0.4890655097627885
1101	38-39	0.5009359672583784
1101	40-41	0.5115840762827375
1101	42-43	0.49655872303399917
1101	44-45	0.4962424928534972
1101	46-47	0.43892114376550495
1101	48-49	0.43054747649183156
1101	50-51	0.4643053349227202
1101	52-53	0.4350576225932343
1101	54-55	0.43019418536409404
1101	56-57	0.40538106716735456
1101	58-59	0.40764183030172063
1101	60-61	0.3997197462846884
1101	62-63	0.3884955479990424
1101	64-65	0.35955429992490906
1101	66-67	0.39355037473325893
1101	68-69	0.3524583514687265
1101	70-71	0.35139737324666953
1101	72-73	0.333566498060911
1101	74-75	0.3393373221720637
1101	76-77	0.3132942653345836
1101	78-79	0.2926571471702246
1101	80-81	0.3460648132558788
1101	82-83	0.3035894170138249
1101	84-85	0.27543747393015394
1101	86-87	0.23285412398767846
1101	88-89	0.2346973059871047
1101	90-91	0.2442672762703566
1101	92-93	0.2198824717573551
1101	94-95	0.2650164285587664
1101	96-97	0.25309262207455063
1101	98-99	0.294364061511601
1101	100	0.22142417423466654
1102	1	0.09427069915966158
1102	2	0.18524094825604465
1102	3	0.2188550689996518
1102	4	0.21982367986753815
1102	5	0.15439206021745377
1102	6	0.19162517767493625
1102	7	0.19897800358323536
1102	8	0.24504836300531707
1102	9	0.31732225453496454
1102	10-11	0.2949801811293753
1102	12-13	0.23506296781633296
1102	14-15	0.33150510518522935
1102	16-17	0.3051134983547428
1102	18-19	0.32422964909571306
1102	20-21	0.28999375320833565
1102	22-23	0.2943507428166967
1102	24-25	0.3185512694474468
1102	26-27	0.29690590120081595
1102	28-29	0.2919309371001404
1102	30-31	0.25575759740370074
1102	32-33	0.2097487207384745
1102	34-35	0.2766640573385928
1102	36-37	0.2620633204664031
1102	38-39	0.29014033091554126
1102	40-41	0.2930348788824091
1102	42-43	0.19924293982180785
1102	44-45	0.1672017335690441
1102	46-47	0.12396626138431799
1102	48-49	0.057342593266547226
1102	50-51	0.07250609000454489
1102	52-53	0.018958439105645652
1102	54-55	0.034296648327490686
1102	56-57	0.03181985475436022
1102	58-59	0.027285616308773797
1102	60-61	-0.025742388302127495
1102	62-63	-0.026904879929652736
1102	64-65	0.0019282972090906014
1102	66-67	0.02998095767239306
1102	68-69	-0.00428653045763383
1102	70-71	-0.055929927455956374
1102	72-73	-0.048374557572003596
1102	74-75	-0.004891886811300594
1102	76-77	-0.05669940491166159
1102	78-79	-0.01954950588359594
1102	80-81	0.04805234004610526
1102	82-83	0.03931608049079216
1102	84-85	-6.380231302927086E-4
1102	86-87	0.022908684598096585
1102	88-89	-0.007672043784126004
1102	90-91	-0.011300493902581366
1102	92-93	-7.415223986910746E-4
1102	94-95	-0.034945438001603435
1102	96-97	-0.012185212804080692
1102	98-99	-0.009359112755639387
1102	100	-0.02250141709680875
1103	1	0.19964296277723292
1103	2	0.2947144839743103
1103	3	0.34263626980219897
1103	4	0.3107421312094658
1103	5	0.2914875915632038
1103	6	0.3389750614750042
1103	7	0.32423112528787357
1103	8	0.33536634412764244
1103	9	0.41580355518774326
1103	10-11	0.4010801493626843
1103	12-13	0.41461060299658925
1103	14-15	0.49715507518756397
1103	16-17	0.4348791588014578
1103	18-19	0.4282446722959321
1103	20-21	0.42245972722427894
1103	22-23	0.40903509708741836
1103	24-25	0.4320167914343571
1103	26-27	0.43964793862727447
1103	28-29	0.41916265481440007
1103	30-31	0.46945232532684145
1103	32-33	0.4210857711639733
1103	34-35	0.45334828523832016
1103	36-37	0.47233872719139214
1103	38-39	0.4499980257756633
1103	40-41	0.49812914394687624
1103	42-43	0.43640129996486365
1103	44-45	0.4101362542200633
1103	46-47	0.3346213924181072
1103	48-49	0.32474749168429184
1103	50-51	0.3540396027151189
1103	52-53	0.33837126821742913
1103	54-55	0.3100101374662003
1103	56-57	0.3004383420551733
1103	58-59	0.29900491342132796
1103	60-61	0.2983089409106725
1103	62-63	0.2810168536929041
1103	64-65	0.37169255832323245
1103	66-67	0.2737655961795582
1103	68-69	0.27687245092756996
1103	70-71	0.26989362709254294
1103	72-73	0.27088804054591975
1103	74-75	0.2493644721008934
1103	76-77	0.1906845257011085
1103	78-79	0.22400902987305216
1103	80-81	0.2070810229985014
1103	82-83	0.21130074031691493
1103	84-85	0.21736307453028303
1103	86-87	0.17606194165179545
1103	88-89	0.213889600275607
1103	90-91	0.1358766538747176
1103	92-93	0.11471417267375728
1103	94-95	0.12591546689113997
1103	96-97	0.13200397171639722
1103	98-99	0.1096116081828491
1103	100	0.10852731052532505
1104	1	0.08773267690511233
1104	2	0.12130921437695719
1104	3	0.14874669051157596
1104	4	0.14158826900553123
1104	5	0.1489920092689303
1104	6	0.1966009558011521
1104	7	0.19566826133360138
1104	8	0.19397131496764786
1104	9	0.29206101711427124
1104	10-11	0.25947744706427756
1104	12-13	0.2579140221108247
1104	14-15	0.36886256678491947
1104	16-17	0.30596407228303235
1104	18-19	0.31122205150052196
1104	20-21	0.27026691142198445
1104	22-23	0.2983543848052932
1104	24-25	0.31943463906805647
1104	26-27	0.3325129601279784
1104	28-29	0.2915937701758793
1104	30-31	0.3048165644751819
1104	32-33	0.3489320518694967
1104	34-35	0.36580191569801457
1104	36-37	0.34511322720089765
1104	38-39	0.2859892798883621
1104	40-41	0.31749753016509885
1104	42-43	0.2968657337691951
1104	44-45	0.25788985631494654
1104	46-47	0.24315354049901572
1104	48-49	0.18421380749960292
1104	50-51	0.1521826301109641
1104	52-53	0.08264701792735707
1104	54-55	0.1212911684810436
1104	56-57	0.15951177015784168
1104	58-59	0.25026231511644426
1104	60-61	0.20759067786151775
1104	62-63	0.24520671484030032
1104	64-65	0.2714385415857734
1104	66-67	0.2878547671035889
1104	68-69	0.2632948713062433
1104	70-71	0.24303878405643786
1104	72-73	0.1659874506430512
1104	74-75	0.21410020278437258
1104	76-77	0.15104629297613315
1104	78-79	0.08433073312101413
1104	80-81	0.11015401056504004
1104	82-83	0.15209689391269166
1104	84-85	0.06991701283332219
1104	86-87	0.05531575531994548
1104	88-89	0.09044378241448925
1104	90-91	0.1046391108936362
1104	92-93	0.09056396454858273
1104	94-95	0.1420793117896899
1104	96-97	0.16854948840375172
1104	98-99	0.14103174179544453
1104	100	0.07328755718261704
1105	1	0.06856062949797348
1105	2	0.07694907004191265
1105	3	0.09789818208362888
1105	4	0.11830044452941735
1105	5	0.07816728325816769
1105	6	0.1414742511379643
1105	7	0.14391030095497825
1105	8	0.14907824409811354
1105	9	0.1418339164226552
1105	10-11	0.19333364960061772
1105	12-13	0.16193851106955037
1105	14-15	0.24761067518754487
1105	16-17	0.20461668130440103
1105	18-19	0.2136433097712569
1105	20-21	0.19427080866442736
1105	22-23	0.16029550458733866
1105	24-25	0.15981075389441202
1105	26-27	0.18197529409685131
1105	28-29	0.16632739774709648
1105	30-31	0.16030232858967963
1105	32-33	0.1355709725341825
1105	34-35	0.17684151145597582
1105	36-37	0.21509335765037463
1105	38-39	0.25213548911313666
1105	40-41	0.2712462880627058
1105	42-43	0.1529401083940911
1105	44-45	0.12180020629463684
1105	46-47	0.1006787188762317
1105	48-49	0.13619766496024965
1105	50-51	0.10223400153098794
1105	52-53	0.06077853303202119
1105	54-55	0.037564315369643
1105	56-57	0.07419673147837358
1105	58-59	0.0798416885363622
1105	60-61	0.05037408000346488
1105	62-63	0.1338935840410045
1105	64-65	0.10501394427493693
1105	66-67	0.0709107691049411
1105	68-69	0.058490297989479245
1105	70-71	0.08064177102484393
1105	72-73	0.06072612565329649
1105	74-75	0.05604128259749075
1105	76-77	0.027946099880821862
1105	78-79	0.03188411502324584
1105	80-81	0.0702405905179262
1105	82-83	0.05121894473989386
1105	84-85	0.004139419265847266
1105	86-87	-0.02833897205430347
1105	88-89	-0.01109925189710026
1105	90-91	-0.008467693424783818
1105	92-93	-0.01954318350257367
1105	94-95	0.010888579910581342
1105	96-97	0.06729107369656973
1105	98-99	0.09988711997275601
1105	100	0.04718519386565845
1106	1	0.1733399568686771
1106	2	0.156036447733495
1106	3	0.16127241875867782
1106	4	0.14853194281635496
1106	5	0.18251476924742605
1106	6	0.17077363839673865
1106	7	0.23380512518090768
1106	8	0.18029331671558424
1106	9	0.19298013140389259
1106	10-11	0.17162986537324798
1106	12-13	0.1830583595252051
1106	14-15	0.22406812727922443
1106	16-17	0.21668944953948
1106	18-19	0.20475082714744275
1106	20-21	0.20728799627059402
1106	22-23	0.23071439837906382
1106	24-25	0.22297017391304763
1106	26-27	0.2661078134559247
1106	28-29	0.17618535291114767
1106	30-31	0.25746334917386804
1106	32-33	0.1770536517005894
1106	34-35	0.23548874324187352
1106	36-37	0.23421310038108345
1106	38-39	0.25637373473309566
1106	40-41	0.3854867522450789
1106	42-43	0.27845666527966983
1106	44-45	0.22223697319945757
1106	46-47	0.23671541103028204
1106	48-49	0.19480979719985925
1106	50-51	0.2715561257752057
1106	52-53	0.2318278916296208
1106	54-55	0.24192233450209955
1106	56-57	0.1818379671992716
1106	58-59	0.2562479855807993
1106	60-61	0.15858454925696464
1106	62-63	0.23127371717748701
1106	64-65	0.2149627756732002
1106	66-67	0.23370196198057158
1106	68-69	0.21424645305728518
1106	70-71	0.22514662497182059
1106	72-73	0.21393979901112203
1106	74-75	0.2050232798860776
1106	76-77	0.1698138968221059
1106	78-79	0.1658617066683732
1106	80-81	0.2542667137624335
1106	82-83	0.21345978197655313
1106	84-85	0.19679302410621347
1106	86-87	0.22303361587120918
1106	88-89	0.24093307851251566
1106	90-91	0.18665522079205488
1106	92-93	0.1027204510588362
1106	94-95	0.15751427283968056
1106	96-97	0.212376452933011
1106	98-99	0.21293802544267137
1106	100	0.21620976446627083
1107	1	0.14432469298771977
1107	2	0.21413046449749729
1107	3	0.20859195795828
1107	4	0.1691400326422965
1107	5	0.1702972860909142
1107	6	0.24701535657343499
1107	7	0.2183337148114859
1107	8	0.22286453577752496
1107	9	0.2466768291058088
1107	10-11	0.2839098074680777
1107	12-13	0.25159533235821385
1107	14-15	0.32124743983236215
1107	16-17	0.25878060938265435
1107	18-19	0.3239182042737383
1107	20-21	0.31387037444095967
1107	22-23	0.2967408277510586
1107	24-25	0.3127885170064033
1107	26-27	0.338631512242614
1107	28-29	0.33539706996446483
1107	30-31	0.3417493545737216
1107	32-33	0.2967028732788748
1107	34-35	0.2973989672230317
1107	36-37	0.2946030955844421
1107	38-39	0.33645885991922597
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2216	78-79	-0.6233273598787079
2216	80-81	-0.5437972945626939
2216	82-83	-0.5058967055009234
2216	84-85	-0.41029973118957486
2216	86-87	-0.5405799049378714
2216	88-89	-0.49068714466565666
2216	90-91	-0.4840712790399486
2216	92-93	-0.527432860417246
2216	94-95	-0.44744407339080894
2216	96-97	-0.45572548429383275
2216	98-99	-0.5070077811620521
2216	100	-0.6010439814803625
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	87645.0
3	17345.0
4	10343.0
5	8535.0
6	7512.0
7	8092.0
8	9148.0
9	10259.0
10	11622.0
11	12841.0
12	14210.0
13	16081.0
14	18071.0
15	18977.0
16	19197.0
17	20226.0
18	19289.0
19	17733.0
20	17974.0
21	19025.0
22	21390.0
23	25946.0
24	31655.0
25	39378.0
26	47615.0
27	54940.0
28	65802.0
29	83943.0
30	101470.0
31	118917.0
32	153328.0
33	205720.0
34	289370.0
35	428383.0
36	730744.0
37	1306471.0
38	1773161.0
39	692774.0
40	1453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37976332225215	20.781637042779014	14.894410883140571	22.944188751828257
2	15.220967318701378	20.451581660404898	40.614900175115274	23.712550845778452
3	18.219950292353367	25.579286266988788	30.441702766145003	25.759060674512842
4	12.570476721758745	17.422947235904886	39.067643270664576	30.938932771671794
5	13.103386970450703	37.99477628302136	35.52837464249699	13.373462104030947
6	30.181629301198036	38.23390500320144	18.106413366349887	13.478052329250634
7	26.428491860491448	32.775255061600994	22.931566554720497	17.864686523187057
8	24.35290618470769	36.59337404329748	20.852947800425703	18.20077197156912
9	24.559974443021893	17.456392182110605	22.05978472407521	35.92384865079229
10-11	23.486770851877317	27.46124153026902	29.13411500419886	19.9178726136548
12-13	23.914232288075908	25.03316842583133	29.073986207504365	21.9786130785884
14-15	21.453395726854687	27.331988460717554	26.2706253963664	24.94399041606136
16-17	20.948269952948202	30.500310904168078	27.052212315721956	21.499206827161764
18-19	20.915294226905875	29.45344325448283	28.89722542859999	20.734037090011295
20-21	22.06007423347704	28.68430697991602	28.373668406308983	20.881950380297965
22-23	21.829082931641864	28.5801848800801	28.350492434164625	21.24023975411341
24-25	21.542756965820516	28.71440872241873	28.355671978239087	21.387162333521662
26-27	21.29976930942531	28.74383753545327	28.460586197471656	21.495806957649762
28-29	21.259321826306437	28.710150175108538	28.6361806930089	21.394347305576126
30-31	21.58608442708086	29.046556017797393	28.187470112190354	21.17988944293139
32-33	21.295047123856214	28.8024809784436	28.500735601417944	21.40173629628224
34-35	21.517776525061922	28.57359786836686	28.47884603806765	21.429779568503562
36-37	21.677376179135223	28.823355621400093	28.1438123457183	21.35545585374638
38-39	21.49007499308949	28.94860562246575	28.229904952852074	21.331414431592684
40-41	21.487948262307942	28.60993662842666	28.393189873890297	21.508925235375102
42-43	21.399198078472768	28.654083329521146	28.39014720050379	21.5565713915023
44-45	21.50294576359755	28.790337604668238	28.207389714137943	21.499326917596274
46-47	21.537718716592842	28.80202106399646	28.131286226432948	21.528973992977747
48-49	21.41115046155699	28.72826263169842	28.16509417470165	21.695492732042947
50-51	21.390860498475842	28.702718759541053	28.145626691091753	21.76079405089136
52-53	21.40984630910672	28.701637767099335	28.123699970822734	21.764815952971208
54-55	21.5224801628072	28.87895322842749	28.037338492179032	21.561228116586282
56-57	21.426246683576935	28.68637283886426	28.087637880494466	21.799742597064334
58-59	21.399792119321184	28.43682388675674	28.31630725094784	21.847076742974238
60-61	21.50487713266979	28.777729584011297	28.0020623872132	21.715330896105716
62-63	21.403744057029563	28.757225378248148	28.09764124942669	21.7413893152956
64-65	21.49850496864566	28.52886313347554	28.091991100202296	21.880640797676502
66-67	21.262858648865027	28.586366246144753	28.0736612718324	22.077113833157824
68-69	21.58917733073915	28.711963244065014	28.055334035533104	21.64352538966273
70-71	21.593880273438174	28.530455364305602	28.033342348983716	21.842322013272515
72-73	21.42951193031102	28.59619857831331	28.183598661752917	21.79069082962276
74-75	21.51842389189558	28.586078821624984	28.03329091202048	21.862206374458953
76-77	21.503161389244124	28.575759548523123	27.86702787795204	22.054051184280716
78-79	21.579974376693208	28.54404551881688	28.095993380505497	21.779986723984415
80-81	21.437402605669405	28.533218428935342	28.086650504155287	21.942728461239962
82-83	21.4272878446416	28.566565269182743	28.20688636385022	21.799260522325437
84-85	21.422511450680677	28.34317341140931	28.15325184642991	22.081063291480106
86-87	21.5985287047626	28.499811643117724	28.00744430466014	21.89421534745954
88-89	21.467654515024094	28.382088091586127	28.054024267175325	22.09623312621445
90-91	21.410215362864683	28.287207099274397	28.10622135160557	22.19635618625535
92-93	21.550724511292046	28.385590342562594	27.98046866453664	22.083216481608726
94-95	21.37139308209878	28.209867362673712	28.181017527785656	22.237722027441848
96-97	21.47398347325945	28.26697814514748	28.06784908329025	22.191189298302817
98-99	21.459404764008514	28.261924766661533	28.042209042287762	22.236461427042194
100	21.530789875740155	28.201269189072086	27.97020015783011	22.297740777357653
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3532.0
1	2614.0
2	1979.5
3	2258.0
4	2132.5
5	1863.0
6	1649.0
7	1616.0
8	1667.0
9	1728.5
10	1588.5
11	1336.5
12	1307.0
13	1405.0
14	1513.5
15	1668.0
16	1959.5
17	2468.0
18	3259.0
19	4378.0
20	6052.5
21	8477.0
22	12340.0
23	18451.0
24	26630.0
25	37057.0
26	51106.5
27	67755.0
28	87218.0
29	109242.0
30	129350.5
31	146806.5
32	163802.0
33	178778.5
34	191728.0
35	202133.5
36	210882.0
37	220120.0
38	226900.5
39	229331.0
40	231820.0
41	232844.5
42	228842.5
43	224260.5
44	218933.0
45	213710.5
46	209996.5
47	204960.0
48	201289.0
49	198046.5
50	192537.5
51	184323.0
52	172841.5
53	163914.5
54	159160.0
55	154138.0
56	141656.0
57	125324.5
58	112734.0
59	102682.0
60	90145.0
61	75630.5
62	63946.5
63	54458.5
64	45643.5
65	37449.5
66	30211.0
67	23693.5
68	18473.5
69	14576.0
70	11249.0
71	8527.5
72	6490.0
73	4814.0
74	3607.0
75	2710.0
76	1957.5
77	1410.0
78	997.0
79	681.0
80	444.5
81	273.0
82	190.5
83	145.5
84	111.5
85	80.0
86	54.0
87	42.0
88	31.5
89	29.5
90	31.0
91	23.0
92	18.0
93	15.0
94	14.0
95	12.0
96	7.5
97	5.0
98	6.5
99	12.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07783881032679908
2	0.013615672403862262
3	0.017929851749805135
4	0.01130559764770136
5	0.009576866207660422
6	0.00719029890990479
7	0.019903359322949215
8	0.009072015433135192
9	0.009194403499686763
10-11	0.013531530608108057
12-13	0.015329105335584254
14-15	0.009944030407315136
16-17	0.012376493230027607
18-19	0.008666604962683113
20-21	0.013072575358539666
22-23	0.013600373895543316
24-25	0.01548973967293319
26-27	0.005209142082601236
28-29	0.01195578425125658
30-31	0.010471828944318784
32-33	0.011879291709661849
34-35	0.008620709437726274
36-37	0.00995932891563408
38-39	0.019115486144523477
40-41	0.012957836546147568
42-43	0.008169403442317357
44-45	0.020691232501374956
46-47	0.0185953368616793
48-49	0.017616232329266734
50-51	0.010425933419361945
52-53	0.010311194606969847
54-55	0.01156567228912345
56-57	0.019979851864543947
58-59	0.0021876866896093296
60-61	0.00719029890990479
62-63	0.015719217297717386
64-65	0.02455410585190891
66-67	0.013118470883496505
68-69	0.014273508261576954
70-71	0.025280784997058863
72-73	0.020186381726849723
74-75	0.020622389213939694
76-77	0.009385634853673592
78-79	0.011297948393541889
80-81	0.01984216528967343
82-83	0.011558023034963975
84-85	0.011152612564511897
86-87	0.017119030808900978
88-89	0.014786008290261658
90-91	0.015107276964959532
92-93	0.008016418359127893
94-95	0.015589179977006342
96-97	0.01337089627075912
98-99	0.009783396069966198
100	0.006777239185293238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6536585.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.5490336229456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2705601023403	42.89920487003979
2	15.667645726339979	18.346510328477585
3	5.170039017947393	9.081023648812279
4	2.178525343675952	5.102022143812898
5	1.114068579432014	3.2613819357716114
6	0.6472084405203573	2.2736057247048356
7	0.4176546927131243	1.711729505250921
8	0.27986748718642	1.3108776733797602
9	0.20478635153350777	1.0791038683310132
>10	0.9789995535973786	9.983957008434462
>50	0.047667220561267105	1.8859217880309895
>100	0.021537716071296332	2.2673603075395796
>500	9.685871036022977E-4	0.38304887614073707
>1k	4.7118097727447806E-4	0.41425232127343226
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.529850831894636E-5	0.0
8	0.0	0.0	0.0	3.059701663789272E-5	0.0
9	3.059701663789272E-5	0.0	0.0	3.059701663789272E-5	0.0
10-11	3.059701663789272E-5	1.529850831894636E-5	0.0	3.059701663789272E-5	0.0
12-13	3.059701663789272E-5	2.2947762478419543E-5	0.0	4.589552495683909E-5	0.0
14-15	3.059701663789272E-5	3.059701663789272E-5	0.0	6.119403327578544E-5	0.0
16-17	3.059701663789272E-5	3.059701663789272E-5	0.0	6.119403327578544E-5	0.0
18-19	3.059701663789272E-5	3.059701663789272E-5	0.0	6.884328743525862E-5	0.0
20-21	3.059701663789272E-5	4.589552495683909E-5	0.0	9.944030407315135E-5	0.0
22-23	3.059701663789272E-5	6.119403327578544E-5	0.0	2.1417911646524906E-4	0.0
24-25	3.059701663789272E-5	6.119403327578544E-5	0.0	5.277985370036495E-4	0.0
26-27	3.059701663789272E-5	6.119403327578544E-5	0.0	0.0011320896156020307	0.0
28-29	3.059701663789272E-5	6.119403327578544E-5	0.0	0.0032432837636166287	0.0
30-31	3.059701663789272E-5	6.119403327578544E-5	0.0	0.008360634796304186	0.0
32-33	3.059701663789272E-5	6.119403327578544E-5	0.0	0.02128022507165439	0.0
34-35	3.059701663789272E-5	6.119403327578544E-5	0.0	0.04328712928845873	0.0
36-37	3.059701663789272E-5	6.119403327578544E-5	0.0	0.07852724320115168	0.0
38-39	3.059701663789272E-5	6.119403327578544E-5	0.0	0.12472873832436968	0.0
40-41	3.82462707973659E-5	6.119403327578544E-5	0.0	0.17225355441717655	0.0
42-43	4.589552495683908E-5	6.119403327578544E-5	0.0	0.2224938557365964	0.0
44-45	4.589552495683908E-5	7.64925415947318E-5	0.0	0.28765785192114846	0.0
46-47	4.589552495683908E-5	9.179104991367816E-5	0.0	0.36875524451988306	0.0
48-49	4.589552495683908E-5	9.179104991367816E-5	0.0	0.4540367791438496	0.0
50-51	4.589552495683908E-5	1.529850831894636E-4	0.0	0.5399837988796903	0.0
52-53	7.64925415947318E-5	1.6828359150840996E-4	0.0	0.6268257813521894	7.64925415947318E-6
54-55	9.179104991367816E-5	1.988806081463027E-4	0.0	0.7287061975022125	1.529850831894636E-5
56-57	1.0708955823262453E-4	2.1417911646524906E-4	0.0	0.8532819507433927	1.529850831894636E-5
58-59	1.223880665515709E-4	2.2947762478419542E-4	0.0	0.9938828914486693	1.529850831894636E-5
60-61	1.223880665515709E-4	2.2947762478419542E-4	0.0	1.14168178031801	1.529850831894636E-5
62-63	1.3768657487051725E-4	2.2947762478419542E-4	0.0	1.2894271244082347	1.529850831894636E-5
64-65	1.3768657487051725E-4	2.2947762478419542E-4	0.0	1.4506428050732914	1.529850831894636E-5
66-67	1.3768657487051725E-4	2.2947762478419542E-4	0.0	1.6410939351358547	1.529850831894636E-5
68-69	1.3768657487051725E-4	2.2947762478419542E-4	0.0	1.8572083129034502	1.529850831894636E-5
70-71	1.3768657487051725E-4	2.2947762478419542E-4	0.0	2.0890878034937206	1.529850831894636E-5
72-73	1.3768657487051725E-4	2.2947762478419542E-4	0.0	2.3235068464649355	1.529850831894636E-5
74-75	1.6828359150840996E-4	2.2947762478419542E-4	0.0	2.567892867605944	1.529850831894636E-5
76-77	1.6828359150840996E-4	2.2947762478419542E-4	0.0	2.834461725809425	1.529850831894636E-5
78-79	1.6828359150840996E-4	2.371268789436686E-4	0.0	3.1383512950569754	1.529850831894636E-5
80-81	1.6828359150840996E-4	2.447761331031418E-4	0.0	3.4689979553543635	1.529850831894636E-5
82-83	1.988806081463027E-4	2.6007464142208814E-4	0.0	3.807997601193896	1.529850831894636E-5
84-85	1.988806081463027E-4	2.753731497410345E-4	0.0	4.152428217486654	1.529850831894636E-5
86-87	1.988806081463027E-4	2.753731497410345E-4	0.0	4.519034021587725	1.529850831894636E-5
88	1.988806081463027E-4	2.753731497410345E-4	0.0	4.816918926320089	1.529850831894636E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6785	0.0	44.988155	1
GTATCAA	11525	0.0	34.484116	1
TCAACGC	12410	0.0	31.205393	4
ATCAACG	12475	0.0	31.008211	3
TATCAAC	14080	0.0	27.87391	2
CAACGCA	14005	0.0	27.719862	5
AACGCAG	14660	0.0	26.418045	6
GTACATG	19730	0.0	24.43431	1
ACGCAGA	16060	0.0	23.823172	7
TACATGG	19940	0.0	23.288734	2
CGCAGAG	16390	0.0	23.168785	8
ACATGGG	20475	0.0	21.922842	3
GTGGTAT	3940	0.0	20.890326	1
GCAGAGT	18790	0.0	19.634678	9
CATGGGG	14370	0.0	18.249527	4
TGGTATC	4175	0.0	18.012648	2
TATACCG	470	0.0	17.999846	5
AGAGTAC	16665	0.0	17.358555	10-11
CAGAGTA	18145	0.0	17.354362	10-11
GAGTACA	14835	0.0	16.105696	1
>>END_MODULE