FastQCFastQC Report
Wed 25 May 2016
SRR2049439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6536585
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234720.359086587262309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226060.34583807905810143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129640.19832986184682064No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67630.10346381176103422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124800.061.2408261
GTATCAA235150.041.888411
GTGGTAT47050.037.1093981
TGGTATC49150.034.046372
ATCAACG296850.032.8884773
TCAACGC299450.032.6186184
TATCAAC305900.032.0998732
CAACGCA314650.031.0727675
AACGCAG325400.030.071226
ACGCAGA361600.026.852817
CGCAGAG365100.026.5310258
GCAGAGT394650.024.1395669
GTACATG195000.023.3620191
TACATGG198900.022.190912
GAGTACT292700.022.07038312-13
CAGAGTA387900.021.63355610-11
ACATGGG198850.021.2745933
AGAGTAC372350.021.17381110-11
GTACTTT315950.020.26777514-15
AGTACTT303150.019.85225512-13
Produced by FastQC (version 0.11.5)