##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6536585 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912241483894114 33.0 31.0 34.0 30.0 34.0 2 32.098722804032995 33.0 31.0 34.0 30.0 34.0 3 32.11903004397556 34.0 31.0 34.0 30.0 34.0 4 35.79293468990306 37.0 35.0 37.0 33.0 37.0 5 35.854282320202365 37.0 35.0 37.0 35.0 37.0 6 35.927346007127575 37.0 35.0 37.0 35.0 37.0 7 35.8894219229154 37.0 35.0 37.0 35.0 37.0 8 35.9102167263181 37.0 35.0 37.0 35.0 37.0 9 37.632487453310866 39.0 37.0 39.0 35.0 39.0 10-11 37.602959343449214 39.0 37.0 39.0 35.0 39.0 12-13 37.57831314057723 39.0 37.0 39.0 35.0 39.0 14-15 38.86106575528353 40.0 38.0 41.0 35.0 41.0 16-17 38.869877390105074 40.0 38.0 41.0 35.0 41.0 18-19 38.952731892876784 40.0 38.5 41.0 35.0 41.0 20-21 38.982164540046526 40.0 39.0 41.0 35.0 41.0 22-23 38.95099711852596 40.0 38.5 41.0 35.0 41.0 24-25 38.926294387665735 40.0 38.0 41.0 35.0 41.0 26-27 38.82070094705416 40.0 38.0 41.0 35.0 41.0 28-29 38.73616437329278 40.0 38.0 41.0 35.0 41.0 30-31 38.61729848231148 40.0 38.0 41.0 35.0 41.0 32-33 38.471203923761415 40.0 38.0 41.0 34.0 41.0 34-35 38.349716709872204 40.0 38.0 41.0 34.0 41.0 36-37 38.20976213114341 40.0 38.0 41.0 34.0 41.0 38-39 38.09633501285457 40.0 38.0 41.0 34.0 41.0 40-41 37.99237682979721 40.0 38.0 41.0 33.0 41.0 42-43 37.833088531702714 40.0 37.5 41.0 33.0 41.0 44-45 37.63210552605068 40.0 37.0 41.0 33.0 41.0 46-47 37.35612181284264 40.0 37.0 41.0 32.5 41.0 48-49 37.08387223603762 40.0 37.0 41.0 31.5 41.0 50-51 36.90870209750199 40.0 37.0 41.0 31.0 41.0 52-53 36.78658565596562 40.0 36.0 41.0 31.0 41.0 54-55 36.64113065155582 40.0 36.0 41.0 31.0 41.0 56-57 36.48240480311968 39.0 35.5 41.0 31.0 41.0 58-59 36.30236981543115 39.0 35.0 41.0 30.5 41.0 60-61 36.11982839663219 39.0 35.0 41.0 30.0 41.0 62-63 35.90445133047302 39.0 35.0 41.0 30.0 41.0 64-65 35.68961881471748 38.0 35.0 40.5 30.0 41.0 66-67 35.434401296701566 38.0 35.0 40.0 29.0 41.0 68-69 35.14907325461231 37.0 35.0 40.0 29.0 41.0 70-71 34.87469573179267 37.0 34.5 39.5 29.0 41.0 72-73 34.54985516137249 36.0 34.0 39.0 29.0 41.0 74-75 34.23298343095057 36.0 34.0 39.0 28.5 41.0 76-77 33.91648857928108 35.0 34.0 38.5 28.0 40.0 78-79 33.61161110885883 35.0 34.0 37.0 28.0 39.5 80-81 33.303605858410776 35.0 34.0 37.0 28.0 39.0 82-83 33.02918213409602 35.0 34.0 36.5 28.0 39.0 84-85 32.74641085520956 35.0 34.0 36.0 27.0 37.5 86-87 32.49622035053473 35.0 33.5 36.0 27.0 37.0 88-89 32.27504178405084 35.0 33.0 35.5 26.5 37.0 90-91 32.07822976370689 35.0 33.0 35.0 26.0 36.0 92-93 31.90988574309062 35.0 33.0 35.0 26.0 36.0 94-95 31.74677434776722 35.0 33.0 35.0 26.0 36.0 96-97 31.596314436360885 35.0 33.0 35.0 25.0 36.0 98-99 31.455970816565532 35.0 33.0 35.0 25.0 35.5 100 31.34287032142931 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3299054794336378 1101 2 0.3754296166956692 1101 3 0.4862396519283436 1101 4 0.28933045317816664 1101 5 0.2744862564552051 1101 6 0.24644951654854452 1101 7 0.2644812735473323 1101 8 0.3008962508706361 1101 9 0.33365734854379525 1101 10-11 0.3390260509265701 1101 12-13 0.33654460409388776 1101 14-15 0.4594446761560107 1101 16-17 0.44148031051329895 1101 18-19 0.42535505305574617 1101 20-21 0.4295618060440205 1101 22-23 0.4254256282150024 1101 24-25 0.40231997332598723 1101 26-27 0.3933101578817144 1101 28-29 0.40506722060843003 1101 30-31 0.4568357744241922 1101 32-33 0.44316978785284533 1101 34-35 0.4357557188003156 1101 36-37 0.4578528741974779 1101 38-39 0.4521992092117202 1101 40-41 0.44487722434082855 1101 42-43 0.4127235583900202 1101 44-45 0.44681677736554093 1101 46-47 0.531851368808212 1101 48-49 0.5240818290870948 1101 50-51 0.5135749895721773 1101 52-53 0.5179695531451145 1101 54-55 0.5166143517468456 1101 56-57 0.5410913068118859 1101 58-59 0.48158272471901853 1101 60-61 0.4052171344222657 1101 62-63 0.43386083849811996 1101 64-65 0.4626547576302471 1101 66-67 0.4736648034442581 1101 68-69 0.3906299195492906 1101 70-71 0.4327489794001025 1101 72-73 0.4506550347485714 1101 74-75 0.40164340351189765 1101 76-77 0.41229558608743844 1101 78-79 0.3944771025038776 1101 80-81 0.3775447557992777 1101 82-83 0.33380884767331054 1101 84-85 0.3182774070102212 1101 86-87 0.3223514641683849 1101 88-89 0.3445359857429864 1101 90-91 0.3650815656835604 1101 92-93 0.3404370657094731 1101 94-95 0.3603422010926103 1101 96-97 0.36467043674717914 1101 98-99 0.44952934072673045 1101 100 0.4726060387861182 1102 1 0.2861290057400545 1102 2 0.30187819583775166 1102 3 0.3729925120852755 1102 4 0.20793621518061656 1102 5 0.21619913509096023 1102 6 0.18150358162286295 1102 7 0.19914292169445247 1102 8 0.1982616075543433 1102 9 0.24913545349456712 1102 10-11 0.24107633900285919 1102 12-13 0.226610565013857 1102 14-15 0.32315977487296266 1102 16-17 0.3081217932208844 1102 18-19 0.3367530587165817 1102 20-21 0.347206287589124 1102 22-23 0.3098074821794228 1102 24-25 0.31644799562451453 1102 26-27 0.3207142974652015 1102 28-29 0.3052019190394617 1102 30-31 0.2636595151120389 1102 32-33 0.3081121080462239 1102 34-35 0.30996796038915875 1102 36-37 0.3287358678949346 1102 38-39 0.34694116399936803 1102 40-41 0.3419977325642449 1102 42-43 0.3047309284716775 1102 44-45 0.3193572251275967 1102 46-47 0.26981718019347056 1102 48-49 0.27152244966116257 1102 50-51 0.2404839108307968 1102 52-53 0.22831680379363206 1102 54-55 0.22280183996669933 1102 56-57 0.21191888785487123 1102 58-59 0.21862205241058774 1102 60-61 0.19565038789384204 1102 62-63 0.16430244557631823 1102 64-65 0.09128605910666465 1102 66-67 0.09336092362497084 1102 68-69 0.09915804623810942 1102 70-71 0.10177503347689765 1102 72-73 0.10525455319472599 1102 74-75 0.08625207199744978 1102 76-77 0.0629837258528454 1102 78-79 0.11741396921816971 1102 80-81 0.08735067049438072 1102 82-83 0.0828059084192816 1102 84-85 0.05314426072934708 1102 86-87 0.09558631789387562 1102 88-89 0.055085939456532174 1102 90-91 0.10176355126793624 1102 92-93 0.04140805827722005 1102 94-95 -4.0965130873971134E-4 1102 96-97 0.018122098938132325 1102 98-99 0.01729405184308419 1102 100 0.05484451538698565 1103 1 0.24201961544332207 1103 2 0.24757388249656032 1103 3 0.3634128945553101 1103 4 0.22737173712580727 1103 5 0.23365410105341766 1103 6 0.20492643253723486 1103 7 0.1981524105230008 1103 8 0.16332612665515 1103 9 0.22007590896937756 1103 10-11 0.2154837915491683 1103 12-13 0.20555301091384592 1103 14-15 0.3169152942297231 1103 16-17 0.32128286773133397 1103 18-19 0.33019994848061884 1103 20-21 0.34055950198222007 1103 22-23 0.31954024860534247 1103 24-25 0.2932306456853837 1103 26-27 0.27729363919984706 1103 28-29 0.2878193712475934 1103 30-31 0.3467236420417663 1103 32-33 0.3499595444196686 1103 34-35 0.32006662417527565 1103 36-37 0.2760075746066235 1103 38-39 0.32789796438951413 1103 40-41 0.34836130520727693 1103 42-43 0.37261428216540793 1103 44-45 0.2941487567782701 1103 46-47 0.2888764314159289 1103 48-49 0.2620437423868225 1103 50-51 0.2602392865234293 1103 52-53 0.29457043669202676 1103 54-55 0.25254680158482046 1103 56-57 0.2663838181567755 1103 58-59 0.24695750346020873 1103 60-61 0.18835806529003918 1103 62-63 0.2463564742077864 1103 64-65 0.2267920333504989 1103 66-67 0.2389737481664298 1103 68-69 0.21796470293197245 1103 70-71 0.18280185343387245 1103 72-73 0.2333807370956933 1103 74-75 0.21873501143983987 1103 76-77 0.23644552477314562 1103 78-79 0.25163913569393515 1103 80-81 0.1708739037950906 1103 82-83 0.15356367297251694 1103 84-85 0.24505926323704585 1103 86-87 0.18631524424719714 1103 88-89 0.17630575790303027 1103 90-91 0.1331267070227895 1103 92-93 0.17274565497432093 1103 94-95 0.19030842523843106 1103 96-97 0.21967745923583237 1103 98-99 0.2737489018835255 1103 100 0.2374576052369548 1104 1 0.2834552131429966 1104 2 0.2887434720405935 1104 3 0.3767800101173506 1104 4 0.25108576248364045 1104 5 0.2097243756778795 1104 6 0.17677370991162178 1104 7 0.17604420232211737 1104 8 0.1585511592021831 1104 9 0.23112650056469874 1104 10-11 0.25204883275568335 1104 12-13 0.23876531528728862 1104 14-15 0.36629819419069065 1104 16-17 0.3533702341938181 1104 18-19 0.2962053275154659 1104 20-21 0.3190102830309698 1104 22-23 0.2900399644204228 1104 24-25 0.257937674013732 1104 26-27 0.32231182515040757 1104 28-29 0.3005200888506039 1104 30-31 0.34687863953029563 1104 32-33 0.3176888239968818 1104 34-35 0.3089944148701491 1104 36-37 0.2924269676710196 1104 38-39 0.27769643604784733 1104 40-41 0.21728581931331803 1104 42-43 0.262982300149055 1104 44-45 0.23671214175215027 1104 46-47 0.25294801238220543 1104 48-49 0.2537034629603028 1104 50-51 0.23446079075010573 1104 52-53 0.27734102654981285 1104 54-55 0.28151929162081046 1104 56-57 0.2495807390697351 1104 58-59 0.2712203622166882 1104 60-61 0.20902324812505668 1104 62-63 0.24730887595860196 1104 64-65 0.21246698786742968 1104 66-67 0.24397059382948072 1104 68-69 0.21910553955034828 1104 70-71 0.20177783140535155 1104 72-73 0.23096779516141908 1104 74-75 0.22211466157619952 1104 76-77 0.1681667725094229 1104 78-79 0.1921523984481439 1104 80-81 0.20493815465813725 1104 82-83 0.16223368658147308 1104 84-85 0.10257750435004453 1104 86-87 0.09117153484093166 1104 88-89 0.12891025004046242 1104 90-91 0.09607485288165662 1104 92-93 0.09062136486711125 1104 94-95 0.11819187051433033 1104 96-97 0.14367826841205655 1104 98-99 0.17300583589663887 1104 100 0.16222867875894664 1105 1 0.20310409887757075 1105 2 0.1801278574362044 1105 3 0.29335051670436485 1105 4 0.17493233886838055 1105 5 0.17648667404716178 1105 6 0.1325908368310209 1105 7 0.13685685408479742 1105 8 0.1214321572770416 1105 9 0.1813279484752428 1105 10-11 0.16598451303991624 1105 12-13 0.1773735842396178 1105 14-15 0.22831088332431193 1105 16-17 0.25943624940943977 1105 18-19 0.21233968459218033 1105 20-21 0.20799351270702005 1105 22-23 0.19893387173049604 1105 24-25 0.24848894128746224 1105 26-27 0.2391920681759956 1105 28-29 0.25430036769378717 1105 30-31 0.2904354698421443 1105 32-33 0.2653250959937523 1105 34-35 0.2599524676678229 1105 36-37 0.26580631416642575 1105 38-39 0.2418642744549473 1105 40-41 0.2796274935551537 1105 42-43 0.23573255624538803 1105 44-45 0.2364968091830093 1105 46-47 0.26093289468235525 1105 48-49 0.301622067703363 1105 50-51 0.21046305235523732 1105 52-53 0.2229294022427908 1105 54-55 0.2442672567711739 1105 56-57 0.17450546577996562 1105 58-59 0.17280588394692842 1105 60-61 0.1460827213364766 1105 62-63 0.17883953443842415 1105 64-65 0.10831472098912798 1105 66-67 0.18762321329727172 1105 68-69 0.1968489359803769 1105 70-71 0.10408232819071372 1105 72-73 0.11221619810774541 1105 74-75 0.18328167417086405 1105 76-77 0.12986868737507962 1105 78-79 0.07261605929439696 1105 80-81 0.08873839161934427 1105 82-83 0.04920459456547377 1105 84-85 0.11895953930049785 1105 86-87 0.09898218897504307 1105 88-89 0.11940768417191094 1105 90-91 0.12824857498663533 1105 92-93 0.15417278029837433 1105 94-95 0.08199336876755225 1105 96-97 0.13204267924788482 1105 98-99 0.1439102999074855 1105 100 0.11303999337949477 1106 1 0.20132037294672145 1106 2 0.16636387260078322 1106 3 0.2477589716221189 1106 4 0.1738254624879545 1106 5 0.18406581494318885 1106 6 0.16560933310043424 1106 7 0.18379064254552446 1106 8 0.1450765933419973 1106 9 0.1756745057639577 1106 10-11 0.18990173302817226 1106 12-13 0.18663552484073875 1106 14-15 0.23228189798562937 1106 16-17 0.22114492899052607 1106 18-19 0.22032238828782624 1106 20-21 0.22466443081670917 1106 22-23 0.21322523517395808 1106 24-25 0.2445054871108283 1106 26-27 0.21062016301154074 1106 28-29 0.2411930318601918 1106 30-31 0.27744674664536717 1106 32-33 0.2565768046234851 1106 34-35 0.24515470226824476 1106 36-37 0.19224502172308178 1106 38-39 0.18946726511556022 1106 40-41 0.22855255983874656 1106 42-43 0.21297823418060347 1106 44-45 0.170133962556676 1106 46-47 0.094168796076751 1106 48-49 0.08997757514681837 1106 50-51 0.11057626943427579 1106 52-53 0.13166314878082375 1106 54-55 0.14795506367632782 1106 56-57 0.1307488366551226 1106 58-59 0.06299690418219228 1106 60-61 0.09674346073077089 1106 62-63 0.16169859284975274 1106 64-65 0.08806684101921292 1106 66-67 0.10290909740751175 1106 68-69 0.14326503510053357 1106 70-71 0.1408869953569436 1106 72-73 0.16776399020090338 1106 74-75 0.14393961915593678 1106 76-77 0.12314216703674674 1106 78-79 0.11460586186507982 1106 80-81 0.0624837123721349 1106 82-83 0.10859997902870333 1106 84-85 0.11923390822355628 1106 86-87 0.11879980163223536 1106 88-89 0.082000115892086 1106 90-91 0.09542184182547686 1106 92-93 0.13887907394106236 1106 94-95 0.07290101220915446 1106 96-97 0.07450651042796608 1106 98-99 0.06322089191210623 1106 100 0.07943307961494028 1107 1 0.14547845685453709 1107 2 0.11581098639896226 1107 3 0.2732286780680582 1107 4 0.14934542825191954 1107 5 0.15693082820373405 1107 6 0.13170609012897216 1107 7 0.10595921368214078 1107 8 0.09231868607010796 1107 9 0.11649689983058664 1107 10-11 0.13589217188779656 1107 12-13 0.13988833983755455 1107 14-15 0.17178031780671432 1107 16-17 0.15174766859362876 1107 18-19 0.13994438479618054 1107 20-21 0.15446512674801482 1107 22-23 0.20619996328608892 1107 24-25 0.17910337854712566 1107 26-27 0.16199360138272567 1107 28-29 0.19272274923154953 1107 30-31 0.17131727003088315 1107 32-33 0.1761895063335075 1107 34-35 0.16364043732796318 1107 36-37 0.13594061437090232 1107 38-39 0.12718489636743158 1107 40-41 0.20993730637218277 1107 42-43 0.1966763111989991 1107 44-45 0.2334757171352848 1107 46-47 0.2134561945904494 1107 48-49 0.2285616385352256 1107 50-51 0.25768415264711564 1107 52-53 0.3036383933783142 1107 54-55 0.3335148377186741 1107 56-57 0.26964486823228384 1107 58-59 0.24482764199706963 1107 60-61 0.25447452430911 1107 62-63 0.301410123175053 1107 64-65 0.2828915857798364 1107 66-67 0.28558736284500696 1107 68-69 0.3150877291495391 1107 70-71 0.3023779128170503 1107 72-73 0.3198543434767558 1107 74-75 0.2927351849694375 1107 76-77 0.3061418165833558 1107 78-79 0.3038696262080549 1107 80-81 0.3057089050188111 1107 82-83 0.27870284574672866 1107 84-85 0.2530318298076466 1107 86-87 0.21081994816290717 1107 88-89 0.23439841399923722 1107 90-91 0.26782564245324636 1107 92-93 0.28616588070524784 1107 94-95 0.30093222183537094 1107 96-97 0.26606923913135105 1107 98-99 0.2850383682352309 1107 100 0.25482098446291346 1108 1 0.03171790021278653 1108 2 0.10012393517177287 1108 3 0.14827186258651892 1108 4 0.03556504594978804 1108 5 0.08784127624622329 1108 6 0.07778204711640058 1108 7 0.07167221715746308 1108 8 0.04531792675241064 1108 9 0.10897839782609253 1108 10-11 0.0760359905919259 1108 12-13 0.03554044102475018 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7 -0.2579368326573004 2215 8 -0.2837000993547747 2215 9 -0.38019204672357887 2215 10-11 -0.4461783495018423 2215 12-13 -0.4745740182344065 2215 14-15 -0.5877015217469932 2215 16-17 -0.5823742898434077 2215 18-19 -0.5439918438732789 2215 20-21 -0.5471423455286555 2215 22-23 -0.5953712148637251 2215 24-25 -0.5804005238299794 2215 26-27 -0.521662256439015 2215 28-29 -0.503609230750115 2215 30-31 -0.5004813979952729 2215 32-33 -0.5001489396723073 2215 34-35 -0.516168439509066 2215 36-37 -0.5004965505124801 2215 38-39 -0.5330017417312618 2215 40-41 -0.6070530166109549 2215 42-43 -0.5828624342700905 2215 44-45 -0.5742304993503353 2215 46-47 -0.5478734020760143 2215 48-49 -0.44521000051549464 2215 50-51 -0.4042351516461693 2215 52-53 -0.5149768504626948 2215 54-55 -0.48620164787246267 2215 56-57 -0.44175218216989975 2215 58-59 -0.4507721095605888 2215 60-61 -0.43220443147189513 2215 62-63 -0.49191623524615835 2215 64-65 -0.5305972723769941 2215 66-67 -0.4702480170586085 2215 68-69 -0.4308281644989975 2215 70-71 -0.427307801220536 2215 72-73 -0.41083423802374597 2215 74-75 -0.3814331024411004 2215 76-77 -0.4226603891704457 2215 78-79 -0.36816434817546906 2215 80-81 -0.45964450989985295 2215 82-83 -0.44987631077380286 2215 84-85 -0.3722893753013139 2215 86-87 -0.3100525389669144 2215 88-89 -0.3436580035610248 2215 90-91 -0.337315468296552 2215 92-93 -0.35248380374203947 2215 94-95 -0.32499853614057983 2215 96-97 -0.2992197276548971 2215 98-99 -0.32125282586171267 2215 100 -0.32767579363031274 2216 1 -0.44321869639444245 2216 2 -0.43098692823347307 2216 3 -0.36805839351796266 2216 4 -0.31802575140721956 2216 5 -0.3310694471995319 2216 6 -0.33961894199968157 2216 7 -0.3593507867254786 2216 8 -0.3258290749179338 2216 9 -0.5477146481530042 2216 10-11 -0.46795695854348196 2216 12-13 -0.5024509310121346 2216 14-15 -0.6923808387255832 2216 16-17 -0.719248793581464 2216 18-19 -0.668898284211096 2216 20-21 -0.6637431929548256 2216 22-23 -0.6219415090516023 2216 24-25 -0.6428421152989259 2216 26-27 -0.6320612600434998 2216 28-29 -0.696543594896724 2216 30-31 -0.6721520180568632 2216 32-33 -0.6997692580804156 2216 34-35 -0.7106381551068637 2216 36-37 -0.7436237782635189 2216 38-39 -0.7177197449948238 2216 40-41 -0.7748595826883076 2216 42-43 -0.846793990770017 2216 44-45 -0.8573738517479086 2216 46-47 -0.8776579016078543 2216 48-49 -0.813292790149923 2216 50-51 -0.8099335036194049 2216 52-53 -0.7892239496847608 2216 54-55 -0.7808196226782016 2216 56-57 -0.7747403322668731 2216 58-59 -0.7560964900946914 2216 60-61 -0.7905550339510938 2216 62-63 -0.8458384668575007 2216 64-65 -0.8651565349912147 2216 66-67 -0.8540096307280294 2216 68-69 -0.8661186933538261 2216 70-71 -0.8726610983341985 2216 72-73 -0.881025733921696 2216 74-75 -0.856919291016375 2216 76-77 -0.7385819607741055 2216 78-79 -0.7450806911264323 2216 80-81 -0.7555095438752559 2216 82-83 -0.7734430210421053 2216 84-85 -0.9052985601662655 2216 86-87 -0.8377003231517186 2216 88-89 -0.8492246255630285 2216 90-91 -0.8354044513845693 2216 92-93 -0.8189510151503114 2216 94-95 -0.8321160556734561 2216 96-97 -0.8830970866436019 2216 98-99 -0.9448908302626542 2216 100 -0.9435665097874484 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 493.0 3 39.0 4 67.0 5 193.0 6 571.0 7 1372.0 8 3054.0 9 4903.0 10 6014.0 11 7801.0 12 10953.0 13 15477.0 14 19128.0 15 25661.0 16 32550.0 17 45881.0 18 46850.0 19 24684.0 20 16926.0 21 16060.0 22 16553.0 23 17793.0 24 20732.0 25 25234.0 26 32429.0 27 41696.0 28 54523.0 29 71397.0 30 93331.0 31 121336.0 32 157449.0 33 206417.0 34 282913.0 35 418939.0 36 703624.0 37 1321005.0 38 1879505.0 39 790663.0 40 2369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.05348099317162 20.859551083292562 15.236052422530818 22.850915501005005 2 15.364536680850934 20.814064224667774 40.11632985725726 23.705069237224023 3 17.760849128405734 25.939676451847564 31.09274950145986 25.206724918286845 4 12.545189881260626 18.050526383424984 38.43817528571877 30.96610844959562 5 12.563692430473465 37.846072023435575 35.6226549970271 13.967580549063866 6 29.70420887278531 38.72419671962613 17.522441426484708 14.049152981103857 7 25.692988005204555 33.53351941418952 22.653266193279826 18.120226387326106 8 24.50834801352694 36.21923374361383 20.41613472478366 18.85628351807557 9 24.69550996430093 16.804906537588053 22.017965038318938 36.481618459792074 10-11 23.53348881717288 27.38165265195817 28.70370384535656 20.38115468551239 12-13 24.477223198352043 25.01384897465573 29.10252065872317 21.406407168269055 14-15 21.66879188444731 26.895940311339945 27.15223622120725 24.2830315830055 16-17 20.305396472316968 30.0845013106997 27.94358216102139 21.666520055961943 18-19 20.177661577107926 28.12027993210522 31.235071524351017 20.466986966435837 20-21 21.29037563192401 27.32982895502774 31.099779166032416 20.280016247015833 22-23 21.03554837885532 27.20013585075387 31.1679569683558 20.59635880203501 24-25 20.82271553112214 27.297671490541315 31.142301369904928 20.737311608431618 26-27 20.59500488404878 27.335030447856184 31.22068327727705 20.84928139081799 28-29 20.57253137532825 27.306269252216563 31.41129504167696 20.70990433077823 30-31 20.8775530341914 27.579523558555426 30.981177480289784 20.56174592696339 32-33 20.6191229824136 27.391298361453266 31.291155855848274 20.698422800284856 34-35 20.85257821936072 27.228698165785346 31.141643534047216 20.77708008080672 36-37 21.014444086629332 27.47833922453391 30.72143175679655 20.7857849320402 38-39 20.908119453812656 27.56173139338049 30.799806014914516 20.73034313789234 40-41 20.872703407054296 27.340201343667985 30.81631310539066 20.97078214388706 42-43 20.921497999337575 27.40577839957715 30.625793132040663 21.046930469044618 44-45 21.079860508201147 27.58927635760875 30.303269979660634 21.027593154529466 46-47 21.234421337747463 27.68525919880182 29.90010838993144 21.180211073519274 48-49 21.25491368964069 27.727024126512546 29.541411914631265 21.4766502692155 50-51 21.292991676846547 27.84774465565735 29.33339809701855 21.52586557047755 52-53 21.268269287403132 27.974255670200876 29.192330551809548 21.565144490586448 54-55 21.402506048647727 28.144015567762064 29.083213941224663 21.370264442365546 56-57 21.266035705188564 27.913941301153432 29.137156481557263 21.682866512100738 58-59 21.232287195836967 27.60952393336888 29.437619184941372 21.72056968585278 60-61 21.288830482583794 27.912878054825264 29.18149920791973 21.616792254671207 62-63 21.146256034305374 27.827175811222528 29.34520089618662 21.68136725828548 64-65 21.18712599927944 27.68262785537096 29.366618807833145 21.763627337516457 66-67 21.084939612963037 27.73492580605928 29.25393764480994 21.926196936167738 68-69 21.43104541591672 27.808649317648282 29.14436207897549 21.615943187459507 70-71 21.38270212962885 27.721623753075956 29.09583521058779 21.799838906707404 72-73 21.230168352434795 27.826617415668885 29.142113198252606 21.801101033643715 74-75 21.402568879817892 27.79862327183256 28.92595513757988 21.87285271076967 76-77 21.401381608286286 27.80348607109064 28.768179408666754 22.026952911956318 78-79 21.426172841017134 27.822984019943135 28.93453538812698 21.81630775091275 80-81 21.23718021091783 27.75612270281037 28.99469966988823 22.011997416383565 82-83 21.21585476609931 27.82803240641406 29.05861360561171 21.89749922187492 84-85 21.202854222579735 27.65081669091744 28.953818376360292 22.192510710142535 86-87 21.301565946720878 27.76766882014492 28.839915264259155 22.09084996887505 88-89 21.12112691742763 27.685882644334015 28.85368398072563 22.339306457512727 90-91 21.03877707815539 27.633196573968316 28.886267387251547 22.441758960624746 92-93 21.066968720923224 27.801689386519605 28.79027300845978 22.341068884097385 94-95 20.879573194985504 27.584903882281957 28.929332640615073 22.606190282117467 96-97 20.918481461933283 27.704653066219947 28.798379879294124 22.578485592552646 98-99 20.84234041140508 27.719066672418958 28.768094856503286 22.670498059672674 100 20.93047769031441 27.62892143259057 28.698089428444057 22.74251144865096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1427.0 1 1052.0 2 1191.5 3 1638.0 4 1039.0 5 684.5 6 1820.5 7 5032.5 8 8622.5 9 9890.5 10 7554.0 11 4600.5 12 4100.5 13 4519.0 14 5013.5 15 5693.5 16 6487.0 17 7372.0 18 8431.0 19 9726.0 20 11550.0 21 13831.0 22 17377.0 23 23154.0 24 30733.5 25 40597.0 26 53963.5 27 69735.5 28 87950.0 29 109164.0 30 128425.0 31 145629.5 32 162629.0 33 177539.0 34 190596.5 35 200755.5 36 209064.0 37 217470.0 38 224353.5 39 227163.0 40 229587.0 41 230705.0 42 227397.5 43 223422.5 44 218654.5 45 213484.0 46 208630.0 47 202765.0 48 198391.5 49 195726.5 50 191196.0 51 182739.0 52 170314.0 53 160218.0 54 155338.0 55 149341.5 56 136116.5 57 120385.0 58 109170.5 59 100536.5 60 88069.0 61 73373.0 62 62018.0 63 52662.5 64 44160.5 65 36085.0 66 28731.5 67 22453.5 68 17444.0 69 13609.0 70 10203.0 71 7505.5 72 5557.0 73 4045.5 74 3017.5 75 2225.5 76 1566.5 77 1114.5 78 748.0 79 455.5 80 249.0 81 127.5 82 81.5 83 41.5 84 20.0 85 11.5 86 7.0 87 5.5 88 3.5 89 2.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12616679810635065 2 0.0 3 0.0 4 0.0 5 0.013447388812353852 6 1.988806081463027E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 7.64925415947318E-6 76-77 0.0 78-79 0.0 80-81 1.6828359150840996E-4 82-83 6.042910785983812E-4 84-85 3.6716419965471264E-4 86-87 4.283582329304981E-4 88-89 4.283582329304981E-4 90-91 6.578358577146935E-4 92-93 1.6828359150840996E-4 94-95 2.6007464142208814E-4 96-97 5.354477911631226E-4 98-99 9.026119908178353E-4 100 1.0708955823262453E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6536585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.89866428486408 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85459944769873 40.165761318502916 2 16.286255396378984 18.207598457194894 3 5.46880768544159 9.170971345409521 4 2.39921578610901 5.364518310986154 5 1.19790407268352 3.348061880220375 6 0.6837606529935456 2.2932784315731394 7 0.4561830613720421 1.7850016660054133 8 0.3070951593881833 1.3732967374517508 9 0.21890318426939495 1.1012756047526382 >10 1.0449076334435556 10.217956445470275 >50 0.05377586614197614 2.0447642291009194 >100 0.026206185668549112 2.689879395601048 >500 0.0015083339802542323 0.573559059274456 >1k 7.678426273607546E-4 0.6553481132777866 >5k 2.742295082701464E-5 0.10367045455548221 >10k+ 8.226885248104392E-5 0.9050585506232115 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23472 0.359086587262309 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22606 0.34583807905810143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12964 0.19832986184682064 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6763 0.10346381176103422 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.529850831894636E-5 0.0 0.0 3.059701663789272E-5 0.0 9 4.589552495683908E-5 0.0 0.0 4.589552495683908E-5 0.0 10-11 4.589552495683908E-5 0.0 0.0 4.589552495683908E-5 0.0 12-13 4.589552495683908E-5 2.294776247841954E-5 0.0 7.64925415947318E-5 0.0 14-15 6.119403327578544E-5 4.589552495683908E-5 0.0 1.0708955823262453E-4 0.0 16-17 6.119403327578544E-5 6.119403327578544E-5 0.0 1.1473881239209771E-4 0.0 18-19 6.884328743525862E-5 7.64925415947318E-5 0.0 1.3003732071104407E-4 0.0 20-21 7.64925415947318E-5 7.64925415947318E-5 0.0 1.6828359150841E-4 0.0 22-23 7.64925415947318E-5 7.64925415947318E-5 0.0 2.8302240390050767E-4 0.0 24-25 7.64925415947318E-5 7.64925415947318E-5 0.0 6.119403327578544E-4 0.0 26-27 7.64925415947318E-5 7.64925415947318E-5 0.0 0.001223880665515709 0.0 28-29 7.64925415947318E-5 7.64925415947318E-5 0.0 0.0033580225760087264 0.0 30-31 7.64925415947318E-5 7.64925415947318E-5 0.0 0.008544216896131543 0.0 32-33 7.64925415947318E-5 9.944030407315135E-5 0.0 0.021517351950598056 0.0 34-35 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.043562502438199764 0.0 36-37 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.07899384770487954 0.0 38-39 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.12529478313217068 0.0 40-41 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.1730414275956023 0.0 42-43 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.2235494528106037 0.0 44-45 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.2887440460117936 0.0 46-47 1.0708955823262453E-4 1.0708955823262453E-4 0.0 0.3698796848813256 0.0 48-49 1.0708955823262453E-4 1.223880665515709E-4 0.0 0.4550388314387406 0.0 50-51 1.0708955823262453E-4 1.6828359150840996E-4 0.0 0.5411082392411328 0.0 52-53 1.223880665515709E-4 1.7593284566788314E-4 0.0 0.6280879082885023 0.0 54-55 1.223880665515709E-4 2.2947762478419542E-4 0.0 0.730297242367383 0.0 56-57 1.223880665515709E-4 2.753731497410345E-4 0.0 0.8552860553331747 0.0 58-59 1.223880665515709E-4 2.753731497410345E-4 0.0 0.9959022945467702 0.0 60-61 1.223880665515709E-4 2.753731497410345E-4 0.0 1.1443054744947094 0.0 62-63 1.3768657487051725E-4 2.753731497410345E-4 0.0 1.292555669359459 0.0 64-65 1.3768657487051725E-4 2.753731497410345E-4 0.0 1.4541997082574463 0.0 66-67 1.3768657487051725E-4 2.753731497410345E-4 0.0 1.6448420696739965 0.0 68-69 1.529850831894636E-4 3.059701663789272E-4 0.0 1.8612318205913332 0.0 70-71 1.529850831894636E-4 3.059701663789272E-4 0.0 2.0930501171483273 0.0 72-73 1.6063433734893678E-4 3.059701663789272E-4 0.0 2.327560951169456 0.0 74-75 1.8358209982735632E-4 3.136194205384004E-4 0.0 2.572207046951887 0.0 76-77 1.8358209982735632E-4 3.2126867469787357E-4 0.0 2.8396861664003454 0.0 78-79 1.8358209982735632E-4 3.2126867469787357E-4 0.0 3.144631332721903 0.0 80-81 1.8358209982735632E-4 3.2126867469787357E-4 0.0 3.4763106423308194 0.0 82-83 1.8358209982735632E-4 3.2126867469787357E-4 0.0 3.8163352882277213 0.0 84-85 1.8358209982735632E-4 3.442164371762931E-4 0.0 4.161630270240501 0.0 86-87 2.0652986230577588E-4 3.518656913357663E-4 0.0 4.529750626665146 0.0 88 2.2947762478419542E-4 3.518656913357663E-4 0.0 4.829020046400376 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12480 0.0 61.240826 1 GTATCAA 23515 0.0 41.88841 1 GTGGTAT 4705 0.0 37.109398 1 TGGTATC 4915 0.0 34.04637 2 ATCAACG 29685 0.0 32.888477 3 TCAACGC 29945 0.0 32.618618 4 TATCAAC 30590 0.0 32.099873 2 CAACGCA 31465 0.0 31.072767 5 AACGCAG 32540 0.0 30.07122 6 ACGCAGA 36160 0.0 26.85281 7 CGCAGAG 36510 0.0 26.531025 8 GCAGAGT 39465 0.0 24.139566 9 GTACATG 19500 0.0 23.362019 1 TACATGG 19890 0.0 22.19091 2 GAGTACT 29270 0.0 22.070383 12-13 CAGAGTA 38790 0.0 21.633556 10-11 ACATGGG 19885 0.0 21.274593 3 AGAGTAC 37235 0.0 21.173811 10-11 GTACTTT 31595 0.0 20.267775 14-15 AGTACTT 30315 0.0 19.852255 12-13 >>END_MODULE