FastQCFastQC Report
Wed 25 May 2016
SRR2049438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402835
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.044.7823641
GTATCAA108700.037.9435461
ATCAACG114900.034.4403883
TCAACGC115300.034.4016234
CAACGCA128600.031.0289065
TATCAAC135200.029.7558982
AACGCAG136400.029.3583566
ACGCAGA152800.025.9001357
CGCAGAG156050.025.1181968
GTACATG190200.022.8470081
TACATGG189250.021.9032552
GCAGAGT179300.021.2056229
ACATGGG193300.020.2286743
AGAGTAC157350.017.9662210-11
GTGGTAT36050.017.8724121
CAGAGTA171550.017.83520710-11
CATGGGG139450.016.8506744
GTACCGT4701.8189894E-1116.000326
TAGACCG3353.0483352E-715.4329025
TGGTATC38500.015.3810432
Produced by FastQC (version 0.11.5)