##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049438_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6402835
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.824130717096413	31.0	31.0	34.0	28.0	34.0
2	30.88454957842893	31.0	31.0	34.0	28.0	34.0
3	31.075572461261302	31.0	31.0	34.0	28.0	34.0
4	34.65626101562823	37.0	35.0	37.0	32.0	37.0
5	34.690193953147315	37.0	35.0	37.0	32.0	37.0
6	34.73046517675373	37.0	35.0	37.0	32.0	37.0
7	34.565420942441904	37.0	35.0	37.0	31.0	37.0
8	34.57368212674542	37.0	35.0	37.0	32.0	37.0
9	36.10772712400054	39.0	37.0	39.0	31.0	39.0
10-11	36.073706803314465	39.0	37.0	39.0	31.0	39.0
12-13	36.00603529530278	39.0	37.0	39.0	31.0	39.0
14-15	37.21349558437785	40.0	38.0	41.0	31.0	41.0
16-17	37.192106152977544	40.0	38.0	41.0	31.0	41.0
18-19	37.19096072286729	40.0	37.5	41.0	31.0	41.0
20-21	37.25452678383872	40.0	38.0	41.0	31.0	41.0
22-23	37.20370171962888	40.0	37.5	41.0	31.0	41.0
24-25	37.20383541040805	40.0	37.0	41.0	31.0	41.0
26-27	37.20748395046881	40.0	37.0	41.0	31.0	41.0
28-29	37.187767918429884	40.0	37.0	41.0	31.0	41.0
30-31	37.13240181888179	40.0	37.0	41.0	31.0	41.0
32-33	37.096616186423674	40.0	37.0	41.0	31.0	41.0
34-35	37.02733523509508	40.0	37.0	41.0	30.5	41.0
36-37	36.91092945546777	40.0	37.0	41.0	30.0	41.0
38-39	36.79754850780943	40.0	37.0	41.0	30.0	41.0
40-41	36.676562725730086	40.0	37.0	41.0	30.0	41.0
42-43	36.56609228568283	40.0	36.5	41.0	30.0	41.0
44-45	36.391625194152276	40.0	36.0	41.0	30.0	41.0
46-47	36.2664112850011	40.0	36.0	41.0	29.5	41.0
48-49	36.16307198295755	40.0	36.0	41.0	29.0	41.0
50-51	36.042237852451294	39.0	35.0	41.0	29.0	41.0
52-53	35.92511973211866	39.0	35.0	41.0	29.0	41.0
54-55	35.7749339940823	39.0	35.0	41.0	28.0	41.0
56-57	35.60599242366858	39.0	35.0	41.0	28.0	41.0
58-59	35.41961443641762	39.0	35.0	40.5	28.0	41.0
60-61	35.22118499071115	38.5	35.0	40.0	28.0	41.0
62-63	34.964666823368084	38.0	34.0	40.0	27.0	41.0
64-65	34.72362601878699	38.0	34.0	40.0	27.0	41.0
66-67	34.50044152316903	37.0	34.0	40.0	26.0	41.0
68-69	34.21828525957642	37.0	34.0	40.0	26.0	41.0
70-71	33.907857534982554	36.0	34.0	39.0	26.0	41.0
72-73	33.57770347041584	36.0	33.0	39.0	26.0	41.0
74-75	33.28380857854373	35.0	33.0	39.0	26.0	40.0
76-77	32.968796712706165	35.0	33.0	37.5	25.5	40.0
78-79	32.63072709198347	35.0	33.0	37.0	25.0	39.0
80-81	32.30883671061335	35.0	33.0	37.0	24.5	39.0
82-83	32.021729827490475	35.0	33.0	36.0	24.0	39.0
84-85	31.740830897563345	35.0	32.0	36.0	24.0	37.0
86-87	31.475442831183372	35.0	32.0	35.5	23.0	37.0
88-89	31.251752153538238	35.0	32.0	35.0	22.5	36.5
90-91	31.04655148852032	35.0	32.0	35.0	20.5	36.0
92-93	30.842870619030478	35.0	32.0	35.0	20.0	36.0
94-95	30.63839486727364	35.0	32.0	35.0	18.5	36.0
96-97	30.450398534399213	35.0	32.0	35.0	16.0	35.5
98-99	30.270572800954575	35.0	32.0	35.0	2.0	35.0
100	30.130657122977556	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4516513033798404
1101	2	0.3736696633375587
1101	3	0.46467530577271177
1101	4	0.4072003179729009
1101	5	0.456664214519499
1101	6	0.45992762131608345
1101	7	0.5584240653654362
1101	8	0.5240160221840355
1101	9	0.6731920811830534
1101	10-11	0.6992874615911546
1101	12-13	0.7154255384726653
1101	14-15	0.8341655788746181
1101	16-17	0.7608921193584663
1101	18-19	0.75806206968916
1101	20-21	0.8027895929628102
1101	22-23	0.7432789067646013
1101	24-25	0.7627378968678755
1101	26-27	0.7674347149408831
1101	28-29	0.7399355065763018
1101	30-31	0.7703020447934534
1101	32-33	0.7645159491725337
1101	34-35	0.6961515981350885
1101	36-37	0.6956515528501441
1101	38-39	0.7063293263645178
1101	40-41	0.7683257434194388
1101	42-43	0.6688901868469941
1101	44-45	0.6249097966336876
1101	46-47	0.5986751915716724
1101	48-49	0.5284566947014895
1101	50-51	0.5776888277305829
1101	52-53	0.5917668618965592
1101	54-55	0.5535494812213315
1101	56-57	0.5368156693411024
1101	58-59	0.568632842256541
1101	60-61	0.5791955198991303
1101	62-63	0.541709981926509
1101	64-65	0.5787683876936924
1101	66-67	0.5919939289749223
1101	68-69	0.5593472810865308
1101	70-71	0.5324883372845903
1101	72-73	0.6179892729053051
1101	74-75	0.5625510049319473
1101	76-77	0.556859191090652
1101	78-79	0.5592044374860734
1101	80-81	0.5625108808602874
1101	82-83	0.4568251356570485
1101	84-85	0.5355740753369425
1101	86-87	0.5363146365837999
1101	88-89	0.4815831420298835
1101	90-91	0.45580202802097247
1101	92-93	0.3961328283995158
1101	94-95	0.39946188318519305
1101	96-97	0.3982574490479358
1101	98-99	0.4596986269925303
1101	100	0.45342550667510295
1102	1	0.32929960028808125
1102	2	0.31418375601666426
1102	3	0.41515460876310684
1102	4	0.4006820892264784
1102	5	0.40841884310656695
1102	6	0.41018943133148866
1102	7	0.42790546741925084
1102	8	0.48415734189357096
1102	9	0.5233949721859119
1102	10-11	0.576001592010627
1102	12-13	0.5777355276333438
1102	14-15	0.6689682643642101
1102	16-17	0.5911383541957278
1102	18-19	0.5875649684546289
1102	20-21	0.6132733081827837
1102	22-23	0.6360616246962039
1102	24-25	0.5873740927014595
1102	26-27	0.5690793684018374
1102	28-29	0.6507643249700124
1102	30-31	0.6054815257339499
1102	32-33	0.6218669095816836
1102	34-35	0.6518054689348673
1102	36-37	0.6804632244175792
1102	38-39	0.7052985639060694
1102	40-41	0.7556301085984245
1102	42-43	0.729838225888642
1102	44-45	0.6886656404795843
1102	46-47	0.62469904512146
1102	48-49	0.6410402096164916
1102	50-51	0.6431589815363381
1102	52-53	0.6264960956296548
1102	54-55	0.6201750664567172
1102	56-57	0.6051046591720279
1102	58-59	0.6429155311317132
1102	60-61	0.6419176704108622
1102	62-63	0.5532455399122611
1102	64-65	0.5729813962606372
1102	66-67	0.5964376347265485
1102	68-69	0.6178251483230852
1102	70-71	0.5752988428940355
1102	72-73	0.5839578010972986
1102	74-75	0.582024907696514
1102	76-77	0.5724413955742378
1102	78-79	0.5921116111401474
1102	80-81	0.59129754736081
1102	82-83	0.5997378764221821
1102	84-85	0.5787978150109048
1102	86-87	0.5964002929466865
1102	88-89	0.5768460873229095
1102	90-91	0.5542120868277927
1102	92-93	0.4980741460050311
1102	94-95	0.5197070759764522
1102	96-97	0.5535529973246547
1102	98-99	0.540488403404499
1102	100	0.5454357622297366
1103	1	0.357867349856015
1103	2	0.3323526042014642
1103	3	0.38548184877883784
1103	4	0.3773589103047357
1103	5	0.3910370046670053
1103	6	0.40589536040649676
1103	7	0.3969527687057166
1103	8	0.39748358569348596
1103	9	0.47946300249608953
1103	10-11	0.513061807514859
1103	12-13	0.5842149170541688
1103	14-15	0.6271220400251067
1103	16-17	0.5944597566356933
1103	18-19	0.5704993981693249
1103	20-21	0.5701875146196471
1103	22-23	0.5955280519849353
1103	24-25	0.6505221331727569
1103	26-27	0.6696248094123263
1103	28-29	0.6091545612353855
1103	30-31	0.6303968317414999
1103	32-33	0.6653351422647233
1103	34-35	0.6334177461229586
1103	36-37	0.6221383245291108
1103	38-39	0.6055464479441284
1103	40-41	0.6491230771508967
1103	42-43	0.6580073885345712
1103	44-45	0.5991772336998338
1103	46-47	0.5594497039063384
1103	48-49	0.5676682147041987
1103	50-51	0.5671121482902279
1103	52-53	0.5699005642414079
1103	54-55	0.527270471799703
1103	56-57	0.5199554432718827
1103	58-59	0.539902073313506
1103	60-61	0.5321457016688953
1103	62-63	0.5632848602202003
1103	64-65	0.56686555593744
1103	66-67	0.5367061547167751
1103	68-69	0.544995654731423
1103	70-71	0.5221164814504817
1103	72-73	0.5372707197935256
1103	74-75	0.5555745358835011
1103	76-77	0.46238464204849095
1103	78-79	0.504653527801544
1103	80-81	0.5316305430582844
1103	82-83	0.47547174633646705
1103	84-85	0.47255570960576776
1103	86-87	0.4469628169457671
1103	88-89	0.4606442083506508
1103	90-91	0.43756739145529977
1103	92-93	0.4402344439770616
1103	94-95	0.47890414347001453
1103	96-97	0.47324812205208033
1103	98-99	0.46804293241588724
1103	100	0.4422760091994533
1104	1	0.2680733334003129
1104	2	0.2515058184256276
1104	3	0.3368660301107411
1104	4	0.34118295725051695
1104	5	0.3705618446100658
1104	6	0.38296014277374724
1104	7	0.37089875467560063
1104	8	0.40672912122730054
1104	9	0.5008666949229337
1104	10-11	0.5193321670339088
1104	12-13	0.5091751820816484
1104	14-15	0.6007044004633286
1104	16-17	0.5214211323274682
1104	18-19	0.5401157083382842
1104	20-21	0.5985062625052393
1104	22-23	0.5055955216491128
1104	24-25	0.5030443198622336
1104	26-27	0.48693968273914834
1104	28-29	0.46074608784557824
1104	30-31	0.48920703793781684
1104	32-33	0.48163222581349885
1104	34-35	0.5283676279287022
1104	36-37	0.5162668041318241
1104	38-39	0.47281185202805887
1104	40-41	0.4201462287565718
1104	42-43	0.4230146546620688
1104	44-45	0.3824291821496928
1104	46-47	0.36315979073815186
1104	48-49	0.2466685742870851
1104	50-51	0.23996491718482105
1104	52-53	0.26171122363981425
1104	54-55	0.29503407903306567
1104	56-57	0.3008166974714612
1104	58-59	0.28181454360535696
1104	60-61	0.32083713912807355
1104	62-63	0.36811433495258683
1104	64-65	0.35593859807246275
1104	66-67	0.3196405425428779
1104	68-69	0.2854438354335258
1104	70-71	0.32161561866433885
1104	72-73	0.27296804462453395
1104	74-75	0.29075206131494014
1104	76-77	0.2805279390192723
1104	78-79	0.2521314474975398
1104	80-81	0.2737597205496698
1104	82-83	0.272128476600372
1104	84-85	0.15150840849872083
1104	86-87	0.17413887851316545
1104	88-89	0.15933907142191117
1104	90-91	0.17993391492712263
1104	92-93	0.17420023892120184
1104	94-95	0.20484943155122792
1104	96-97	0.2637648149959553
1104	98-99	0.2342637375836638
1104	100	0.19420913954931152
1105	1	0.2740505955774317
1105	2	0.2548810987961474
1105	3	0.3090299334442079
1105	4	0.29197284275566915
1105	5	0.3342593975838639
1105	6	0.32426451707164716
1105	7	0.2968075568844881
1105	8	0.3200311311321187
1105	9	0.3816609288810824
1105	10-11	0.46145105403478226
1105	12-13	0.4592736908729549
1105	14-15	0.5096029729393905
1105	16-17	0.4310396520257527
1105	18-19	0.4615825732061012
1105	20-21	0.4950145605849201
1105	22-23	0.4818849415249673
1105	24-25	0.5164680375152741
1105	26-27	0.48403153387934594
1105	28-29	0.4864915755431838
1105	30-31	0.5020903456481705
1105	32-33	0.508841262128712
1105	34-35	0.405747404469885
1105	36-37	0.46043955846197804
1105	38-39	0.5228382204279498
1105	40-41	0.5988560902081019
1105	42-43	0.5148392500630479
1105	44-45	0.48815490322073174
1105	46-47	0.4281635525312453
1105	48-49	0.47121425995823074
1105	50-51	0.503114571909002
1105	52-53	0.3948769292881309
1105	54-55	0.3721930415634276
1105	56-57	0.3377208037835686
1105	58-59	0.346927688167348
1105	60-61	0.3605513862465273
1105	62-63	0.4724917169426135
1105	64-65	0.45285483817307437
1105	66-67	0.43957296455608486
1105	68-69	0.4028371011695242
1105	70-71	0.49369914788290004
1105	72-73	0.4806808698751013
1105	74-75	0.49661105831655306
1105	76-77	0.4368486561487117
1105	78-79	0.4444586755846487
1105	80-81	0.4524732749428324
1105	82-83	0.39030598885052115
1105	84-85	0.375597745593506
1105	86-87	0.3636990804607372
1105	88-89	0.35002188779462884
1105	90-91	0.3859169583503146
1105	92-93	0.3488248590859371
1105	94-95	0.3579767613336422
1105	96-97	0.37298495221406114
1105	98-99	0.4093602178176674
1105	100	0.3540556315335799
1106	1	0.23575999149012716
1106	2	0.24142311209153178
1106	3	0.23311503677295775
1106	4	0.24495643161481695
1106	5	0.2270792815414211
1106	6	0.24594468979298512
1106	7	0.2621926365323546
1106	8	0.21030649972841786
1106	9	0.29907086964955454
1106	10-11	0.32542317976668755
1106	12-13	0.28852061974465926
1106	14-15	0.363133512847682
1106	16-17	0.3726431804920409
1106	18-19	0.37610955194323736
1106	20-21	0.3476963494625238
1106	22-23	0.39029018332499277
1106	24-25	0.3811658018796251
1106	26-27	0.39816568552066656
1106	28-29	0.3517346875807803
1106	30-31	0.3411812808126058
1106	32-33	0.32782405913779655
1106	34-35	0.3138574707624855
1106	36-37	0.34010852884892273
1106	38-39	0.35069561422103135
1106	40-41	0.4351962030566767
1106	42-43	0.3491499209549431
1106	44-45	0.29882112045358156
1106	46-47	0.3096340334426344
1106	48-49	0.23847187279866944
1106	50-51	0.29937310756778146
1106	52-53	0.2783207339996494
1106	54-55	0.29930889731995336
1106	56-57	0.23130376253597262
1106	58-59	0.27481172087404815
1106	60-61	0.2702329274780624
1106	62-63	0.3544080618265042
1106	64-65	0.3085920574766732
1106	66-67	0.33658406645740513
1106	68-69	0.31001014747903355
1106	70-71	0.31692234672463826
1106	72-73	0.2950632660779675
1106	74-75	0.3317482228499742
1106	76-77	0.27648983572304076
1106	78-79	0.2320419593871037
1106	80-81	0.2750340541319929
1106	82-83	0.21935174623405373
1106	84-85	0.2520075213497286
1106	86-87	0.27510976933065123
1106	88-89	0.2513190333336013
1106	90-91	0.22998005212474126
1106	92-93	0.14698760349430628
1106	94-95	0.18638252856128545
1106	96-97	0.28624306545434663
1106	98-99	0.28374623116550524
1106	100	0.2502469131238172
1107	1	0.14723537892894356
1107	2	0.17586049796057424
1107	3	0.18356634802719896
1107	4	0.15727464062909036
1107	5	0.1861812019719835
1107	6	0.1936367888688224
1107	7	0.196147805586385
1107	8	0.14459582999147358
1107	9	0.21974876526412146
1107	10-11	0.23325540508149345
1107	12-13	0.2728940309288461
1107	14-15	0.290433946269566
1107	16-17	0.2576983925823768
1107	18-19	0.24883924633062549
1107	20-21	0.329994953482327
1107	22-23	0.27044034506032233
1107	24-25	0.28819335351482067
1107	26-27	0.30802270803377496
1107	28-29	0.27186699866798847
1107	30-31	0.2738968118554652
1107	32-33	0.2177656120362812
1107	34-35	0.23135334019491438
1107	36-37	0.2350918042544592
1107	38-39	0.22252019076851326
1107	40-41	0.29319484870303114
1107	42-43	0.2959672508163038
1107	44-45	0.28344045884136904
1107	46-47	0.22491772738943183
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2216	4	-0.8300913627696005
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2216	82-83	-1.3664535775553333
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2216	86-87	-1.3529741733533918
2216	88-89	-1.3308917662891844
2216	90-91	-1.346446383466887
2216	92-93	-1.3291963120594978
2216	94-95	-1.298446838161123
2216	96-97	-1.3290594745162174
2216	98-99	-1.3672724680371964
2216	100	-1.4683116903735822
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107646.0
3	20532.0
4	12726.0
5	10441.0
6	8853.0
7	8860.0
8	9399.0
9	10871.0
10	12355.0
11	13612.0
12	14961.0
13	16677.0
14	18960.0
15	19921.0
16	19968.0
17	20843.0
18	19969.0
19	18405.0
20	18673.0
21	20019.0
22	23059.0
23	27504.0
24	34041.0
25	42516.0
26	51496.0
27	59579.0
28	71779.0
29	89821.0
30	108217.0
31	127528.0
32	162750.0
33	217212.0
34	300259.0
35	442218.0
36	737597.0
37	1285323.0
38	1646772.0
39	570482.0
40	991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.77136267468264	20.493622270349217	14.719685621255014	23.015329433713124
2	15.309935515393411	20.374530243941223	40.6229903719045	23.69254386876087
3	18.212194823633354	25.458058414973785	30.338693378950033	25.99105338244283
4	12.661344487936846	17.318427900670713	38.92078273827189	31.099444873120554
5	13.19403161318196	37.92857091536028	35.30487486774214	13.572522603715623
6	30.373757986256727	38.058151585224614	18.009794756814042	13.558295671704618
7	26.716829141723675	32.56183846595803	22.796651711025902	17.92468068129239
8	24.449399276507744	36.46180735612938	20.778956871614486	18.30983649574839
9	24.609260599926433	17.500017571851302	21.951100271231287	35.93962155699098
10-11	23.50622520710599	27.328071882132498	29.113581991728882	20.05212091903263
12-13	24.01746349730745	24.895254161834135	29.007821866079343	22.07946047477907
14-15	21.57686352157096	27.14658986870795	26.21724522466972	25.059301385051374
16-17	21.144015962940784	30.272270169758148	26.89160246941677	21.692111397884297
18-19	21.077294492446534	29.291512904178337	28.7418454435033	20.889347159871832
20-21	22.17250880072827	28.583704817239912	28.26979642541339	20.97398995661843
22-23	21.89159897398225	28.422469204511348	28.274156953411755	21.411774868094646
24-25	21.67733060059206	28.489390099727824	28.294056686053437	21.53922261362668
26-27	21.449452715493276	28.538341153170936	28.313390664925684	21.6988154664101
28-29	21.433651223072374	28.556117782951418	28.473762186402464	21.536468807573748
30-31	21.774434770194524	28.85607318337595	28.021971756979884	21.347520289449644
32-33	21.440117286323	28.635869374638258	28.40727288290322	21.51674045613552
34-35	21.678365696514454	28.343973891304724	28.40513930007935	21.572521112101466
36-37	21.867971655372894	28.609356376033805	27.99750429578828	21.525167672805022
38-39	21.69565446610757	28.772327608931235	28.084860437189413	21.44715748777178
40-41	21.661290267154882	28.436069111569505	28.259180569236058	21.643460052039547
42-43	21.563822536738655	28.498775566879697	28.194824697585673	21.742577198795974
44-45	21.68898730197783	28.606490187004397	28.10524733122839	21.599275179789387
46-47	21.72265837893414	28.622301929581457	27.99197438325858	21.663065308225825
48-49	21.659592036425053	28.493014720354502	28.07021613471821	21.77717710850224
50-51	21.67745708375477	28.443169259482882	28.006202859528862	21.873170797233485
52-53	21.64700615759584	28.508448848781683	27.99621659767448	21.848328395948002
54-55	21.789070160387464	28.740060471849116	27.850515900706835	21.62035346705659
56-57	21.69879294765446	28.502739125683924	27.911385716650084	21.88708221001153
58-59	21.623127742983428	28.262522061005512	28.17631624158558	21.938033954425475
60-61	21.817635005891933	28.552058592434882	27.862457440741135	21.767848960932053
62-63	21.62507793567065	28.634114060723554	27.914930760165575	21.825877243440225
64-65	21.73190336547444	28.370153136304243	27.986710940619457	21.91123255760186
66-67	21.561868585989533	28.40277945367339	27.961741001846363	22.073610958490715
68-69	21.881431879684754	28.535921524517104	27.883354575563985	21.69929202023415
70-71	21.859218490798952	28.343155065368947	27.90959340794444	21.88803303588766
72-73	21.659434506717016	28.462983830094736	28.07191060942517	21.805671053763078
74-75	21.72766794803893	28.535332687505253	27.888174215182595	21.848825149273218
76-77	21.799323145581493	28.486911581108114	27.711099965746488	22.002665307563905
78-79	21.825624210762502	28.493616474917115	27.926431526186263	21.754327788134116
80-81	21.708337261377654	28.443159630071563	27.9638353843908	21.884667724159986
82-83	21.665219490472147	28.516587388277294	28.046274649831	21.77191847141956
84-85	21.677968213009773	28.379625742768344	27.928394339555595	22.014011704666288
86-87	21.805157946712267	28.553623341894973	27.829640706356372	21.811578005036385
88-89	21.642298178078413	28.467601939065588	27.86630853617324	22.023791346682756
90-91	21.579061232555	28.35427111148947	27.964449644285565	22.102218011669965
92-93	21.682477760938504	28.581823174294605	27.802446628673387	21.933252436093497
94-95	21.590373738812705	28.381978236474648	27.988509355966602	22.039138668746048
96-97	21.61047944406288	28.466379121961367	27.92389400906183	21.99924742491392
98-99	21.543226701300405	28.56771892940512	27.84240191120757	22.046652458086903
100	21.597847182979137	28.439326365323513	27.84461206903284	22.118214382664505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3378.0
1	2574.5
2	2070.0
3	2287.5
4	2003.0
5	1651.5
6	1454.5
7	1400.5
8	1436.5
9	1509.5
10	1308.0
11	1095.0
12	1136.0
13	1194.0
14	1325.0
15	1491.0
16	1728.0
17	2160.0
18	2789.0
19	3837.5
20	5590.5
21	8105.5
22	11927.0
23	18181.5
24	26801.0
25	37354.5
26	50697.5
27	66234.0
28	84271.0
29	104358.5
30	122871.0
31	139441.0
32	154689.5
33	169515.0
34	182838.5
35	193198.0
36	201760.0
37	210149.0
38	218352.0
39	222749.0
40	225027.5
41	224938.0
42	222165.0
43	218983.0
44	214470.5
45	211016.5
46	208568.5
47	203403.5
48	199328.0
49	196917.0
50	191409.5
51	182460.0
52	169985.5
53	160690.5
54	155082.5
55	148365.5
56	138141.5
57	124985.0
58	113014.5
59	102717.0
60	91597.0
61	78649.5
62	67176.5
63	57002.5
64	47338.0
65	38973.5
66	31557.5
67	25150.0
68	20006.5
69	15789.5
70	12187.0
71	9558.0
72	7615.0
73	5925.0
74	4687.5
75	3538.5
76	2495.5
77	1875.5
78	1433.5
79	1030.0
80	705.5
81	435.0
82	283.5
83	202.0
84	154.0
85	121.0
86	84.5
87	69.5
88	56.0
89	44.0
90	32.0
91	22.0
92	16.5
93	18.0
94	16.0
95	12.0
96	15.0
97	12.5
98	8.5
99	10.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07726265006048103
2	0.013150424772776434
3	0.016961236702179583
4	0.010854566766127817
5	0.008886688474714717
6	0.006715775121489153
7	0.019319567035539727
8	0.008668035331224372
9	0.008589944922834963
10-11	0.012478847260627519
12-13	0.014212454326872393
14-15	0.009081914495688239
16-17	0.011815078789317545
18-19	0.007957412614880753
20-21	0.012619409995728454
22-23	0.012517892464822223
24-25	0.015001167451605422
26-27	0.0049196957285327515
28-29	0.01124501880807486
30-31	0.009620738313575157
32-33	0.011315300175625329
34-35	0.008176065758371097
36-37	0.00990186378377703
38-39	0.017921748725369308
40-41	0.012197721790425647
42-43	0.0075669605729337085
44-45	0.019483556893157485
46-47	0.01693000053882382
48-49	0.01697685478385746
50-51	0.009784728171192917
52-53	0.010323551989079837
54-55	0.010659340745154296
56-57	0.019499174974835366
58-59	0.002108441026514036
60-61	0.006848528815751148
62-63	0.014376444184490151
64-65	0.02309914280158711
66-67	0.012268003157976114
68-69	0.013845429407442173
70-71	0.024270498927428243
72-73	0.01906186868785468
74-75	0.019280521831345022
76-77	0.00893354271974836
78-79	0.010784285398577348
80-81	0.019030632524498912
82-83	0.011120074154651808
84-85	0.010737431153543704
86-87	0.01599291563815091
88-89	0.01382981132576429
90-91	0.014524815960430029
92-93	0.00740297071531595
94-95	0.014540434042107909
96-97	0.012744354649151508
98-99	0.00937084900672905
100	0.006762629366522797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6402835.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.21384909610876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69518331085001	44.22989312774854
2	14.842728620594844	17.57790185428795
3	4.76417889344316	8.463161101896292
4	2.037668222635363	4.826327145722662
5	1.0666919473113783	3.158146800006518
6	0.6014589190755693	2.136881860298967
7	0.40105229151781707	1.6623494908709249
8	0.28722619208446554	1.3606214715631595
9	0.19907446457263533	1.0609168773683413
>10	1.028427527103105	10.733200957041372
>50	0.0540165206654274	2.1730659167618174
>100	0.021369849484408617	2.176927927334697
>500	7.913566057657392E-4	0.2996308555519665
>1k	1.3188405612107976E-4	0.14097461354659244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5618081677881752E-5
2	0.0	1.5618081677881752E-5	0.0	0.0	1.5618081677881752E-5
3	0.0	3.1236163355763504E-5	0.0	0.0	1.5618081677881752E-5
4	0.0	4.685424503364525E-5	0.0	3.1236163355763504E-5	1.5618081677881752E-5
5	0.0	4.685424503364525E-5	0.0	3.1236163355763504E-5	1.5618081677881752E-5
6	0.0	4.685424503364525E-5	0.0	3.1236163355763504E-5	1.5618081677881752E-5
7	0.0	4.685424503364525E-5	0.0	4.685424503364525E-5	1.5618081677881752E-5
8	0.0	4.685424503364525E-5	0.0	4.685424503364525E-5	1.5618081677881752E-5
9	0.0	4.685424503364525E-5	0.0	4.685424503364525E-5	1.5618081677881752E-5
10-11	0.0	4.685424503364525E-5	0.0	7.809040838940875E-5	1.5618081677881752E-5
12-13	0.0	4.685424503364525E-5	0.0	7.809040838940875E-5	1.5618081677881752E-5
14-15	0.0	4.685424503364525E-5	0.0	1.0932657174517226E-4	3.1236163355763504E-5
16-17	0.0	4.685424503364525E-5	0.0	1.0932657174517226E-4	3.1236163355763504E-5
18-19	0.0	6.247232671152701E-5	0.0	1.2494465342305402E-4	3.1236163355763504E-5
20-21	0.0	6.247232671152701E-5	0.0	1.4056273510093577E-4	3.1236163355763504E-5
22-23	0.0	6.247232671152701E-5	0.0	1.4837177593987665E-4	3.1236163355763504E-5
24-25	0.0	7.028136755046787E-5	0.0	2.655073885239898E-4	3.1236163355763504E-5
26-27	0.0	7.809040838940875E-5	0.0	3.982610827859847E-4	3.1236163355763504E-5
28-29	0.0	7.809040838940875E-5	0.0	9.44893941511846E-4	3.1236163355763504E-5
30-31	0.0	7.809040838940875E-5	0.0	0.002655073885239898	3.1236163355763504E-5
32-33	0.0	7.809040838940875E-5	0.0	0.00633313212038105	3.1236163355763504E-5
34-35	0.0	9.37084900672905E-5	0.0	0.012556937669016928	3.1236163355763504E-5
36-37	0.0	1.1713561258411314E-4	0.0	0.02149048038876529	3.1236163355763504E-5
38-39	0.0	1.2494465342305402E-4	0.0	0.03289168001361897	3.1236163355763504E-5
40-41	0.0	1.2494465342305402E-4	0.0	0.04462085935370816	3.1236163355763504E-5
42-43	0.0	1.2494465342305402E-4	0.0	0.05692009867504004	3.1236163355763504E-5
44-45	0.0	1.2494465342305402E-4	0.0	0.07235076337278722	3.1236163355763504E-5
46-47	0.0	1.2494465342305402E-4	0.0	0.09088161728359391	3.1236163355763504E-5
48-49	0.0	1.2494465342305402E-4	0.0	0.10955303392950154	3.1236163355763504E-5
50-51	0.0	1.87416980134581E-4	0.0	0.12786523469681788	3.1236163355763504E-5
52-53	0.0	2.264621843292854E-4	0.0	0.14592754615728815	3.1236163355763504E-5
54-55	0.0	2.4208026600716715E-4	0.0	0.167870950914712	3.1236163355763504E-5
56-57	0.0	2.5769834768504894E-4	0.0	0.19290673584435644	3.1236163355763504E-5
58-59	0.0	2.655073885239898E-4	0.0	0.22093338341531527	3.1236163355763504E-5
60-61	0.0	2.7331642936293064E-4	0.0	0.25081077366510307	3.1236163355763504E-5
62-63	0.0	2.8112547020187154E-4	0.0	0.28157839457053013	3.1236163355763504E-5
64-65	0.0	2.8112547020187154E-4	0.0	0.3151728882596537	3.1236163355763504E-5
66-67	0.0	2.8112547020187154E-4	0.0	0.3549052880481849	3.1236163355763504E-5
68-69	0.0	2.8112547020187154E-4	0.0	0.3998385090354507	3.1236163355763504E-5
70-71	0.0	2.8112547020187154E-4	0.0	0.4490823205658119	3.1236163355763504E-5
72-73	0.0	2.8112547020187154E-4	0.0	0.4989040011182546	3.1236163355763504E-5
74-75	0.0	2.8112547020187154E-4	0.0	0.553137789744699	3.1236163355763504E-5
76-77	0.0	3.12361633557635E-4	0.0	0.6138608913083032	3.1236163355763504E-5
78-79	0.0	3.2017067439657585E-4	0.0	0.6838064701026967	3.1236163355763504E-5
80-81	0.0	3.3578875607445766E-4	0.0	0.7616782253486152	3.1236163355763504E-5
82-83	0.0	3.5140683775233936E-4	0.0	0.8455160877954844	3.1236163355763504E-5
84-85	0.0	3.5921587859128026E-4	0.0	0.9313921099013172	3.1236163355763504E-5
86-87	0.0	3.5921587859128026E-4	0.0	1.025717514194884	3.1236163355763504E-5
88	0.0	3.5921587859128026E-4	0.0	1.1040109576461052	3.1236163355763504E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6070	0.0	44.782364	1
GTATCAA	10870	0.0	37.943546	1
ATCAACG	11490	0.0	34.440388	3
TCAACGC	11530	0.0	34.401623	4
CAACGCA	12860	0.0	31.028906	5
TATCAAC	13520	0.0	29.755898	2
AACGCAG	13640	0.0	29.358356	6
ACGCAGA	15280	0.0	25.900135	7
CGCAGAG	15605	0.0	25.118196	8
GTACATG	19020	0.0	22.847008	1
TACATGG	18925	0.0	21.903255	2
GCAGAGT	17930	0.0	21.205622	9
ACATGGG	19330	0.0	20.228674	3
AGAGTAC	15735	0.0	17.96622	10-11
GTGGTAT	3605	0.0	17.872412	1
CAGAGTA	17155	0.0	17.835207	10-11
CATGGGG	13945	0.0	16.850674	4
GTACCGT	470	1.8189894E-11	16.00032	6
TAGACCG	335	3.0483352E-7	15.432902	5
TGGTATC	3850	0.0	15.381043	2
>>END_MODULE