FastQCFastQC Report
Wed 25 May 2016
SRR2049438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402835
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219570.34292621940124957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203120.3172344750411341No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121810.1902438529182776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119650.060.4989051
GTATCAA221250.040.715761
GTGGTAT49250.033.2565271
ATCAACG278450.031.7031483
TCAACGC282150.031.3540464
TATCAAC289350.030.7525522
TGGTATC49450.030.608612
CAACGCA292900.030.2193415
AACGCAG303200.029.2812086
ACGCAGA340050.025.9837177
CGCAGAG344200.025.5748528
GCAGAGT377350.023.0042849
GTACATG180200.021.469451
GAGTACT285100.021.4634712-13
CAGAGTA366850.021.22213610-11
AGAGTAC354250.020.69669210-11
TACATGG181650.020.0548932
GTACTTT310900.019.55383314-15
AGTACTT299450.019.21855412-13
ACATGGG184450.019.036893
Produced by FastQC (version 0.11.5)