##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6402835 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.961449264271216 33.0 31.0 34.0 30.0 34.0 2 32.06147339420741 33.0 31.0 34.0 30.0 34.0 3 32.09108387144132 34.0 31.0 34.0 30.0 34.0 4 35.799061509471976 37.0 35.0 37.0 33.0 37.0 5 35.850343324480484 37.0 35.0 37.0 35.0 37.0 6 35.92578896691856 37.0 35.0 37.0 35.0 37.0 7 35.89662344883165 37.0 35.0 37.0 35.0 37.0 8 35.893348961827066 37.0 35.0 37.0 35.0 37.0 9 37.582055761237015 39.0 37.0 39.0 35.0 39.0 10-11 37.57155166422373 39.0 37.0 39.0 35.0 39.0 12-13 37.55547862782658 39.0 37.0 39.0 35.0 39.0 14-15 38.84232679742645 40.0 38.0 41.0 35.0 41.0 16-17 38.85091705471092 40.0 38.0 41.0 35.0 41.0 18-19 38.93625066084008 40.0 38.5 41.0 35.0 41.0 20-21 38.969054411053854 40.0 38.5 41.0 35.0 41.0 22-23 38.93744193002006 40.0 38.0 41.0 35.0 41.0 24-25 38.921884290318275 40.0 38.0 41.0 35.0 41.0 26-27 38.81849657222153 40.0 38.0 41.0 35.0 41.0 28-29 38.73556729167626 40.0 38.0 41.0 35.0 41.0 30-31 38.61515703590675 40.0 38.0 41.0 35.0 41.0 32-33 38.47065346834644 40.0 38.0 41.0 34.0 41.0 34-35 38.35091002657417 40.0 38.0 41.0 34.0 41.0 36-37 38.21359710190876 40.0 38.0 41.0 34.0 41.0 38-39 38.0992860974865 40.0 38.0 41.0 34.0 41.0 40-41 37.99359580560798 40.0 38.0 41.0 33.0 41.0 42-43 37.8398624203185 40.0 37.5 41.0 33.0 41.0 44-45 37.64356640456923 40.0 37.0 41.0 33.0 41.0 46-47 37.37623099767525 40.0 37.0 41.0 32.5 41.0 48-49 37.10916789203533 40.0 37.0 41.0 32.0 41.0 50-51 36.939283145669066 40.0 37.0 41.0 31.0 41.0 52-53 36.81834374929231 40.0 36.0 41.0 31.0 41.0 54-55 36.672913795217276 40.0 36.0 41.0 31.0 41.0 56-57 36.51935595716585 39.0 35.5 41.0 31.0 41.0 58-59 36.33918279949428 39.0 35.0 41.0 31.0 41.0 60-61 36.14918883588285 39.0 35.0 41.0 30.0 41.0 62-63 35.93353412980344 39.0 35.0 41.0 30.0 41.0 64-65 35.71619454507261 38.0 35.0 40.0 30.0 41.0 66-67 35.456443356731825 38.0 35.0 40.0 29.5 41.0 68-69 35.16547935406737 37.0 35.0 40.0 29.0 41.0 70-71 34.8859296702164 37.0 34.5 39.5 29.0 41.0 72-73 34.55874881985871 36.0 34.0 39.0 29.0 41.0 74-75 34.2404609832988 36.0 34.0 39.0 29.0 41.0 76-77 33.927149458013524 35.0 34.0 38.5 29.0 40.0 78-79 33.624899907619046 35.0 34.0 37.0 28.0 39.0 80-81 33.32062929936505 35.0 34.0 37.0 28.0 39.0 82-83 33.049092472318904 35.0 34.0 36.5 28.0 39.0 84-85 32.77121938953604 35.0 34.0 36.0 27.5 37.0 86-87 32.5275858272156 35.0 33.5 36.0 27.0 37.0 88-89 32.313047189252885 35.0 33.0 35.0 27.0 37.0 90-91 32.12501829267816 35.0 33.0 35.0 26.5 36.0 92-93 31.96241953759546 35.0 33.0 35.0 26.0 36.0 94-95 31.802959079845103 35.0 33.0 35.0 26.0 36.0 96-97 31.65953011439464 35.0 33.0 35.0 26.0 36.0 98-99 31.52645991970744 35.0 33.0 35.0 25.0 35.5 100 31.417305771583994 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3354321391281516 1101 2 0.38560715162991244 1101 3 0.5237528654152399 1101 4 0.3025002595691504 1101 5 0.2912842384857939 1101 6 0.2776651822093754 1101 7 0.3039536931033595 1101 8 0.2898795831180152 1101 9 0.3189136691445569 1101 10-11 0.32853842507064 1101 12-13 0.29542582622408986 1101 14-15 0.4321229856138302 1101 16-17 0.4236564419125486 1101 18-19 0.3697265143467945 1101 20-21 0.39857719735262975 1101 22-23 0.43291448196507787 1101 24-25 0.41167195818510294 1101 26-27 0.42064330911404824 1101 28-29 0.4459529458738487 1101 30-31 0.4512732383312468 1101 32-33 0.41871669714237214 1101 34-35 0.4637819026260672 1101 36-37 0.43358814353791786 1101 38-39 0.4262178699257788 1101 40-41 0.5000231313170502 1101 42-43 0.5186707833842803 1101 44-45 0.44292156663757964 1101 46-47 0.47561407722847093 1101 48-49 0.47597240655019135 1101 50-51 0.40117164050491283 1101 52-53 0.4191755833349404 1101 54-55 0.39901950018331434 1101 56-57 0.42111979842272973 1101 58-59 0.3417933041059271 1101 60-61 0.31119204699873393 1101 62-63 0.3480102519482884 1101 64-65 0.3136615355047141 1101 66-67 0.36464527613568265 1101 68-69 0.35508631822142434 1101 70-71 0.3310539088465845 1101 72-73 0.3724967933580956 1101 74-75 0.3917141980129131 1101 76-77 0.41547583821991196 1101 78-79 0.3822638554381541 1101 80-81 0.3351406558071872 1101 82-83 0.3879085209728288 1101 84-85 0.3667071739549286 1101 86-87 0.33463298255600904 1101 88-89 0.3550733430843991 1101 90-91 0.38019194577960036 1101 92-93 0.4213698278870339 1101 94-95 0.41938991267808134 1101 96-97 0.366632572820631 1101 98-99 0.49085799994992385 1101 100 0.5195997497443976 1102 1 0.2677541560074843 1102 2 0.30721534913786286 1102 3 0.3509300593151252 1102 4 0.24523700415971206 1102 5 0.2643122628205248 1102 6 0.236092567214655 1102 7 0.275670859017211 1102 8 0.22396214403620007 1102 9 0.3221382923513687 1102 10-11 0.3091235344855363 1102 12-13 0.31047579532567227 1102 14-15 0.3715446121665167 1102 16-17 0.3955425634293448 1102 18-19 0.40796973865064956 1102 20-21 0.3953068835404707 1102 22-23 0.36341212253249466 1102 24-25 0.3435216900009195 1102 26-27 0.3062302613574772 1102 28-29 0.3599220714210034 1102 30-31 0.33575823953924555 1102 32-33 0.4265377486637192 1102 34-35 0.43112827236512885 1102 36-37 0.3875041020940131 1102 38-39 0.3900917148388743 1102 40-41 0.3798054551138108 1102 42-43 0.321707091680679 1102 44-45 0.378831758239599 1102 46-47 0.3840596084304124 1102 48-49 0.3573824985994847 1102 50-51 0.29666504320722 1102 52-53 0.3163731576257547 1102 54-55 0.34627742786889115 1102 56-57 0.3583240978116464 1102 58-59 0.385666669468236 1102 60-61 0.3309624469838184 1102 62-63 0.3228636877828208 1102 64-65 0.24971937077906858 1102 66-67 0.27019506979232233 1102 68-69 0.28965132845419106 1102 70-71 0.27215315441847565 1102 72-73 0.2719820746491166 1102 74-75 0.2601415264710525 1102 76-77 0.2711901665802401 1102 78-79 0.19716664540796103 1102 80-81 0.25750824773902536 1102 82-83 0.2201628132726512 1102 84-85 0.17979393381516218 1102 86-87 0.21630498361054862 1102 88-89 0.22851266309432106 1102 90-91 0.23911294330683575 1102 92-93 0.20814573482581267 1102 94-95 0.18865640584467158 1102 96-97 0.21232365564877398 1102 98-99 0.2301860506935931 1102 100 0.22452095661250127 1103 1 0.2610959633973664 1103 2 0.2988189391512037 1103 3 0.3849130603815425 1103 4 0.21467637586937371 1103 5 0.23055739510023443 1103 6 0.2254948176087197 1103 7 0.2287136913789496 1103 8 0.21304490342210158 1103 9 0.2768642741351286 1103 10-11 0.2940581083158307 1103 12-13 0.2653485007934151 1103 14-15 0.32584908937006674 1103 16-17 0.33388735633694466 1103 18-19 0.3565903310998735 1103 20-21 0.320994243760822 1103 22-23 0.34923959455644393 1103 24-25 0.3606435391396161 1103 26-27 0.36374091559085997 1103 28-29 0.3640766467967822 1103 30-31 0.3955545119528878 1103 32-33 0.3781404683202112 1103 34-35 0.378449141860564 1103 36-37 0.35452657555725864 1103 38-39 0.2954428669402063 1103 40-41 0.3456688748129153 1103 42-43 0.34837264953657154 1103 44-45 0.3242309312366558 1103 46-47 0.335410908224155 1103 48-49 0.32193965649093315 1103 50-51 0.29648765583814196 1103 52-53 0.3297753303732591 1103 54-55 0.3141949046268948 1103 56-57 0.2557097522592642 1103 58-59 0.2757068064808834 1103 60-61 0.2197634805027633 1103 62-63 0.30322987552222713 1103 64-65 0.30103474972372624 1103 66-67 0.31519240693257444 1103 68-69 0.2821796167283068 1103 70-71 0.21433714874982712 1103 72-73 0.25437398441398074 1103 74-75 0.26184622989286055 1103 76-77 0.26056914908689066 1103 78-79 0.2490894857400292 1103 80-81 0.21461718534256136 1103 82-83 0.19987265656892106 1103 84-85 0.2048777596862692 1103 86-87 0.16903227435735602 1103 88-89 0.1845300964611667 1103 90-91 0.17224625105108515 1103 92-93 0.1857340833110328 1103 94-95 0.1976478515334712 1103 96-97 0.2288118879845733 1103 98-99 0.3039145156024503 1103 100 0.30194854989027675 1104 1 0.2342812932667897 1104 2 0.2364438622877003 1104 3 0.2767315923046283 1104 4 0.21212738004181375 1104 5 0.1747109816735346 1104 6 0.1405404888903874 1104 7 0.15777203104222082 1104 8 0.1475365617375104 1104 9 0.21735181746061016 1104 10-11 0.2098801807473123 1104 12-13 0.2141051738219346 1104 14-15 0.3093001914171296 1104 16-17 0.26879210942691856 1104 18-19 0.24579869555095257 1104 20-21 0.32075157850695746 1104 22-23 0.2513761339222569 1104 24-25 0.22435130676822013 1104 26-27 0.21746472614812973 1104 28-29 0.22689237227120884 1104 30-31 0.24421671468529382 1104 32-33 0.22852930720793552 1104 34-35 0.24603880885277363 1104 36-37 0.26868903467864413 1104 38-39 0.2607518422566528 1104 40-41 0.2582843828354484 1104 42-43 0.2504302508846905 1104 44-45 0.2897294854581389 1104 46-47 0.265432249149157 1104 48-49 0.26621835237531144 1104 50-51 0.24004867535136043 1104 52-53 0.256292653119111 1104 54-55 0.24134617838011252 1104 56-57 0.2570543581968536 1104 58-59 0.2698389031895303 1104 60-61 0.2128074666386155 1104 62-63 0.19055404693900613 1104 64-65 0.15338959207110037 1104 66-67 0.18602512854946696 1104 68-69 0.17934784832521444 1104 70-71 0.22531602272347584 1104 72-73 0.228671893513976 1104 74-75 0.19384495752265707 1104 76-77 0.20071030132662315 1104 78-79 0.24597983849297123 1104 80-81 0.2154630562033688 1104 82-83 0.22170721014357753 1104 84-85 0.20296282125153198 1104 86-87 0.1855860660418429 1104 88-89 0.19434479425196116 1104 90-91 0.14875963753459587 1104 92-93 0.18784413269516165 1104 94-95 0.14556769646601353 1104 96-97 0.165588493394484 1104 98-99 0.20065135412420432 1104 100 0.1722572166888625 1105 1 0.20383787738340686 1105 2 0.2332100289790091 1105 3 0.3479773927926857 1105 4 0.19548695090036716 1105 5 0.21013595390250117 1105 6 0.1682448004594903 1105 7 0.187768572536946 1105 8 0.15994682581641229 1105 9 0.18749918119532794 1105 10-11 0.19878077438340824 1105 12-13 0.19417212019621388 1105 14-15 0.24443877573791184 1105 16-17 0.26105506667312284 1105 18-19 0.21969482749901914 1105 20-21 0.2404268374936933 1105 22-23 0.16814843920437283 1105 24-25 0.24336460169951124 1105 26-27 0.2666322210238192 1105 28-29 0.26861967658427943 1105 30-31 0.2534016921401445 1105 32-33 0.26182333262015334 1105 34-35 0.25889124721279444 1105 36-37 0.28715979153137994 1105 38-39 0.30821101305981813 1105 40-41 0.28102257557922883 1105 42-43 0.253954586118212 1105 44-45 0.22622156987389275 1105 46-47 0.24463431883525288 1105 48-49 0.2814807907888195 1105 50-51 0.24099448948452817 1105 52-53 0.21332156151173365 1105 54-55 0.2054218453316281 1105 56-57 0.2188017534780542 1105 58-59 0.21503313997002493 1105 60-61 0.16542352600699672 1105 62-63 0.20102570443704337 1105 64-65 0.1734627441805756 1105 66-67 0.18704115978548685 1105 68-69 0.20905347820050935 1105 70-71 0.21493773007338746 1105 72-73 0.25266977197875207 1105 74-75 0.20178235800812416 1105 76-77 0.1987345775527416 1105 78-79 0.17749355307672232 1105 80-81 0.2140761147864012 1105 82-83 0.13494362339023525 1105 84-85 0.1667626044092856 1105 86-87 0.15735011882279792 1105 88-89 0.1804895373068689 1105 90-91 0.2354166069576138 1105 92-93 0.23966743869617346 1105 94-95 0.1189289493485326 1105 96-97 0.2058418530893924 1105 98-99 0.25319085750378534 1105 100 0.21198268488728544 1106 1 0.206572099650991 1106 2 0.16733909423228255 1106 3 0.2356562362443384 1106 4 0.14800433933991286 1106 5 0.14818003228275956 1106 6 0.14042300849571632 1106 7 0.14555216251535086 1106 8 0.12390123668741637 1106 9 0.16505573825381958 1106 10-11 0.17495622963196666 1106 12-13 0.13923851866827874 1106 14-15 0.22645157967161111 1106 16-17 0.22643020849943696 1106 18-19 0.2084385845900556 1106 20-21 0.19200411911842252 1106 22-23 0.15458731012616056 1106 24-25 0.15388652176346795 1106 26-27 0.17948940145909376 1106 28-29 0.22586032284292656 1106 30-31 0.23416432367484674 1106 32-33 0.23410753113189742 1106 34-35 0.21530738184236498 1106 36-37 0.24597824184436945 1106 38-39 0.20688590615522173 1106 40-41 0.21210775826620676 1106 42-43 0.2335486519795893 1106 44-45 0.21833078411210494 1106 46-47 0.1885759890859191 1106 48-49 0.14753902071579006 1106 50-51 0.254948895358865 1106 52-53 0.24472166876692114 1106 54-55 0.232864734253468 1106 56-57 0.2474070344097754 1106 58-59 0.19854402331144883 1106 60-61 0.1954397666281551 1106 62-63 0.20240032167962596 1106 64-65 0.22413971580190406 1106 66-67 0.23555038717494625 1106 68-69 0.27112307961580484 1106 70-71 0.298488970963632 1106 72-73 0.27868875428386986 1106 74-75 0.23070263457180573 1106 76-77 0.24144958307145004 1106 78-79 0.2281655835465486 1106 80-81 0.16701617154568282 1106 82-83 0.19815526926458915 1106 84-85 0.2138177508467507 1106 86-87 0.1615740816109721 1106 88-89 0.1501582344314869 1106 90-91 0.16339715312228975 1106 92-93 0.16795091075871937 1106 94-95 0.1751175922528354 1106 96-97 0.1726530878555046 1106 98-99 0.2230421329521164 1106 100 0.19127854947992518 1107 1 0.08798250709314459 1107 2 0.10534243271161614 1107 3 0.19171366741746 1107 4 0.11123885058715643 1107 5 0.11983890859868751 1107 6 0.10242137345536406 1107 7 0.11906377346433317 1107 8 0.1058794941054586 1107 9 0.06685708254781986 1107 10-11 0.05673612343048262 1107 12-13 0.06368968390103191 1107 14-15 0.13499704119458045 1107 16-17 0.12658814209351021 1107 18-19 0.16266395409164147 1107 20-21 0.15502501520215617 1107 22-23 0.11167166155254193 1107 24-25 0.11759017489821844 1107 26-27 0.13952553742597473 1107 28-29 0.18498230708696184 1107 30-31 0.1296347108349778 1107 32-33 0.0739165975955629 1107 34-35 0.08902019765135094 1107 36-37 0.07613681526143523 1107 38-39 0.04578148443229679 1107 40-41 0.08414061449303745 1107 42-43 0.06552338939218316 1107 44-45 0.10707879845266177 1107 46-47 0.11696507338804452 1107 48-49 0.11474956629530908 1107 50-51 0.11113203510712566 1107 52-53 0.10631118226605452 1107 54-55 0.16418942804018855 1107 56-57 0.17005141761453757 1107 58-59 0.1394472001446374 1107 60-61 0.149974371218633 1107 62-63 0.20037680260443835 1107 64-65 0.14987549810178535 1107 66-67 0.15083430955224486 1107 68-69 0.12337047038457882 1107 70-71 0.09536637696241712 1107 72-73 0.12169740888514724 1107 74-75 0.1686004218372119 1107 76-77 0.16408017342422454 1107 78-79 0.17540162654004376 1107 80-81 0.17578806100846123 1107 82-83 0.218386011060268 1107 84-85 0.1737502609426187 1107 86-87 0.14933111434471158 1107 88-89 0.1962947695687376 1107 90-91 0.19212253500263898 1107 92-93 0.14746362517937683 1107 94-95 0.15314723243909611 1107 96-97 0.12274585022393936 1107 98-99 0.14259022756098716 1107 100 0.16230086609688854 1108 1 0.03995131154892562 1108 2 0.06612428655034819 1108 3 0.1360594687473764 1108 4 0.06855002052221693 1108 5 0.05857598710661449 1108 6 0.0895612792196232 1108 7 0.051686879164158483 1108 8 0.04678318570434925 1108 9 0.09802689954667443 1108 10-11 0.08528704947232058 1108 12-13 0.05158903465395781 1108 14-15 0.13303251972467933 1108 16-17 0.1249563265672009 1108 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-0.28297294009733065 2215 5 -0.283331272510317 2215 6 -0.28656520582200073 2215 7 -0.2990917904466599 2215 8 -0.3368269852717063 2215 9 -0.4293398747805881 2215 10-11 -0.4360272838796604 2215 12-13 -0.48634410146460993 2215 14-15 -0.6375969684589933 2215 16-17 -0.6328655734767494 2215 18-19 -0.6162187212729933 2215 20-21 -0.5647527582717515 2215 22-23 -0.5886279266340537 2215 24-25 -0.5885582125351121 2215 26-27 -0.5226101763026705 2215 28-29 -0.510072980552799 2215 30-31 -0.524129506121831 2215 32-33 -0.5568834492127124 2215 34-35 -0.5816310069234518 2215 36-37 -0.6018543304943336 2215 38-39 -0.5844441536514182 2215 40-41 -0.6391865702450872 2215 42-43 -0.5682010802284054 2215 44-45 -0.570859504334706 2215 46-47 -0.5701114732830632 2215 48-49 -0.5200106978553478 2215 50-51 -0.4900992125493957 2215 52-53 -0.5751413680689836 2215 54-55 -0.5609897742734518 2215 56-57 -0.5175353600563142 2215 58-59 -0.5309739051644087 2215 60-61 -0.5132513713451914 2215 62-63 -0.5289428971873988 2215 64-65 -0.5917931591039363 2215 66-67 -0.49039271140885177 2215 68-69 -0.44758226653799227 2215 70-71 -0.4030261375273412 2215 72-73 -0.4415618760241813 2215 74-75 -0.4415467990252111 2215 76-77 -0.4644785319692417 2215 78-79 -0.42593026113878807 2215 80-81 -0.5082084186499358 2215 82-83 -0.5083315829824073 2215 84-85 -0.4717081952642985 2215 86-87 -0.4718893658497336 2215 88-89 -0.4234171769825643 2215 90-91 -0.4697922251802602 2215 92-93 -0.4097297284756358 2215 94-95 -0.4560465053732585 2215 96-97 -0.4457646826402133 2215 98-99 -0.39034980827539556 2215 100 -0.35963296356149854 2216 1 -0.35411996459916395 2216 2 -0.3139806350371792 2216 3 -0.26432721456960095 2216 4 -0.25711282908355315 2216 5 -0.31609420288329915 2216 6 -0.2979835830022779 2216 7 -0.32173172549867246 2216 8 -0.2611832723175951 2216 9 -0.46857331730665663 2216 10-11 -0.4657043544921251 2216 12-13 -0.44802916491887856 2216 14-15 -0.6428731990170036 2216 16-17 -0.6623159661534146 2216 18-19 -0.5754519564871643 2216 20-21 -0.5683831292292396 2216 22-23 -0.5868890306622703 2216 24-25 -0.6112477135433139 2216 26-27 -0.6115672257229647 2216 28-29 -0.6960427417796566 2216 30-31 -0.6992829756562244 2216 32-33 -0.6986917530795012 2216 34-35 -0.7082485397188876 2216 36-37 -0.7771906186412281 2216 38-39 -0.6874626799057779 2216 40-41 -0.7554980430630707 2216 42-43 -0.8541250963239406 2216 44-45 -0.8326980214495521 2216 46-47 -0.8030874840904616 2216 48-49 -0.7852869255560577 2216 50-51 -0.8058717601624039 2216 52-53 -0.8405019809172316 2216 54-55 -0.8129516733951618 2216 56-57 -0.7656084071163605 2216 58-59 -0.7509937480556488 2216 60-61 -0.7299840349436408 2216 62-63 -0.7930525986411254 2216 64-65 -0.7886702244843988 2216 66-67 -0.7733858754499536 2216 68-69 -0.8290816928269251 2216 70-71 -0.8381525949251909 2216 72-73 -0.7596167196395811 2216 74-75 -0.787643227184148 2216 76-77 -0.6603627464035213 2216 78-79 -0.6884800755200402 2216 80-81 -0.6867809470492858 2216 82-83 -0.7110098970852619 2216 84-85 -0.8110879085541711 2216 86-87 -0.7318627029369082 2216 88-89 -0.7321247384441918 2216 90-91 -0.6783622290096574 2216 92-93 -0.6909717737150913 2216 94-95 -0.732059354139011 2216 96-97 -0.7998443568868758 2216 98-99 -0.8767807051256966 2216 100 -0.9180964732077257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 434.0 3 40.0 4 83.0 5 150.0 6 535.0 7 1256.0 8 2508.0 9 4333.0 10 5761.0 11 7545.0 12 10895.0 13 15353.0 14 19355.0 15 24489.0 16 31350.0 17 44029.0 18 43616.0 19 22689.0 20 15862.0 21 14773.0 22 15822.0 23 17064.0 24 19758.0 25 24403.0 26 31255.0 27 40553.0 28 53928.0 29 70208.0 30 91364.0 31 118390.0 32 154312.0 33 203548.0 34 280838.0 35 417432.0 36 696091.0 37 1309859.0 38 1848263.0 39 742554.0 40 2137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.56587349228511 20.564794115480968 15.122033062423254 22.747299329810673 2 15.509083085851813 20.710013611158182 40.0520394481507 23.728863854839304 3 17.911628208442043 25.752842295639354 30.88400685009062 25.45152264582798 4 12.682897497749043 17.860650789845437 38.29381828518149 31.162633427224034 5 12.708476092190187 37.60686510844154 35.47180438708283 14.212854412285445 6 29.9055370975745 38.499407527701415 17.498603352173436 14.09645202255065 7 25.93109146182902 33.223767284335764 22.63044417043388 18.214697083401337 8 24.633603708357313 35.9396111253843 20.452159082656355 18.97462608360203 9 24.720377770159622 16.797387407296924 21.972485625508074 36.509749197035376 10-11 23.528233977605232 27.1844971797649 28.748265416803648 20.539003425826216 12-13 24.52620596969936 24.814414239942153 29.086076714455395 21.573303075903098 14-15 21.72921057625255 26.641761032417673 27.118830955350248 24.51019743597953 16-17 20.507618890694513 29.82691260980487 27.79536720843189 21.87010129106872 18-19 20.35930177804051 27.94076373981213 31.075187787909574 20.624746694237786 20-21 21.363185214049714 27.2305002393471 30.966142341634605 20.44017220496858 22-23 21.098989744386667 27.049556954068006 31.072485859779302 20.77896744176603 24-25 20.95180494265431 27.109983312079727 31.00643699236354 20.931774752902424 26-27 20.735517938538166 27.14127413872136 31.077639826733 21.04556809600747 28-29 20.72683428512526 27.196124841574083 31.207246477536906 20.86979439576375 30-31 21.035299207304263 27.48341945403872 30.753361596855143 20.727919741801873 32-33 20.727302827575596 27.29133266748245 31.087291801209933 20.894072703732018 34-35 21.003977144499274 27.059451008810942 30.980518785819093 20.95605306087069 36-37 21.23285232244779 27.339451977132008 30.490275010991226 20.93742068942898 38-39 21.076484088688836 27.49748353658965 30.53912837047964 20.88690400424187 40-41 21.00627300250592 27.29826709574743 30.56151689056488 21.133943011181767 42-43 21.06474710030791 27.40385313693075 30.336889830832746 21.19450993192859 44-45 21.242676095823178 27.52020784543097 30.075185445197324 21.16193061354853 46-47 21.38621407548375 27.68279207569772 29.62851611825074 21.302477730567787 48-49 21.458072869283683 27.657592300910455 29.364070759280853 21.520264070525013 50-51 21.51313441623906 27.722899309446518 29.13585934980364 21.628106924510785 52-53 21.501413045939806 27.89577585553899 28.98552750461319 21.61728359390801 54-55 21.635712930287912 28.127017172861706 28.815673057325387 21.42159683952499 56-57 21.520662331607795 27.84703182262232 28.936596054716386 21.695709791053496 58-59 21.438667402798917 27.5816181425884 29.216690731527517 21.763023723085166 60-61 21.57269397071766 27.85907336359597 28.942819860265022 21.625412805421348 62-63 21.405408697865866 27.870818161017734 29.061283009791754 21.662490131324642 64-65 21.46834956702773 27.67206245358501 29.158630512889992 21.70095746649726 66-67 21.401785302916597 27.719517994763258 29.071629988903354 21.80706671341679 68-69 21.743836909743887 27.839745987519592 28.88508762134273 21.531329481393787 70-71 21.70998471770708 27.654218795268033 28.912661344545032 21.723135142479855 72-73 21.526612820727067 27.828430687343964 28.981529275703654 21.663427216225312 74-75 21.646463241625757 27.87407325210003 28.749943286827623 21.729520219446595 76-77 21.71431092633185 27.865437731879705 28.62621010849101 21.79404123329744 78-79 21.760501402894185 27.87832264926396 28.771177142625103 21.58999880521675 80-81 21.63615913835446 27.790081820998047 28.858119279814037 21.71563976083345 82-83 21.575743377396172 27.881996708464868 28.915999476790383 21.626260437348577 84-85 21.608224636300424 27.759904554436392 28.778511691425578 21.853359117837602 86-87 21.657667881211815 27.907745483876333 28.69672738142957 21.73785925348228 88-89 21.50272755498997 27.839466896066163 28.72013574052868 21.93766980841519 90-91 21.395398924421254 27.77227192758527 28.80123814861516 22.031090999378318 92-93 21.490446513446145 27.948702911272132 28.690485496272384 21.870365079009332 94-95 21.349274244716675 27.736514227146277 28.842504758650982 22.071706769486067 96-97 21.379288263953477 27.838123819363265 28.734786917275972 22.04780099940729 98-99 21.313320412350073 27.944082270101028 28.667730738453795 22.074866579095104 100 21.386528602609005 27.78314259721295 28.642013486234593 22.188315313943455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1419.0 1 1053.0 2 1306.0 3 1833.5 4 1147.5 5 739.5 6 1889.5 7 5005.5 8 8388.0 9 9586.5 10 7323.5 11 4479.5 12 4030.0 13 4510.5 14 5130.5 15 5837.0 16 6558.5 17 7453.5 18 8455.0 19 9495.5 20 11252.0 21 13867.5 22 17462.0 23 23276.5 24 31254.5 25 41212.0 26 54134.0 27 68685.5 28 85177.5 29 104467.0 30 122398.5 31 138702.0 32 153901.0 33 167221.5 34 180502.0 35 191108.0 36 198569.5 37 207159.0 38 215514.0 39 218977.5 40 221164.0 41 222911.5 42 220982.5 43 218156.5 44 214261.5 45 210064.0 46 206629.0 47 201613.5 48 196757.5 49 193219.5 50 188911.0 51 180539.0 52 167855.0 53 158143.5 54 152146.5 55 144400.5 56 133665.5 57 120767.5 58 109051.0 59 100688.0 60 90330.5 61 76635.5 62 65111.5 63 55010.5 64 45177.0 65 37032.5 66 30160.0 67 23862.0 68 18659.5 69 14581.0 70 11142.0 71 8539.0 72 6618.0 73 5156.5 74 3939.5 75 2864.5 76 2017.0 77 1483.0 78 1099.0 79 739.0 80 444.5 81 227.0 82 120.0 83 65.0 84 37.0 85 26.5 86 20.5 87 11.5 88 6.5 89 3.0 90 3.0 91 2.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 3.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11679201478719972 2 0.0 3 0.0 4 0.0 5 0.011276254971430623 6 6.2472326711527E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.3427122516822626E-5 76-77 0.0 78-79 0.0 80-81 2.576983476850489E-4 82-83 7.418588796993832E-4 84-85 3.5921587859128026E-4 86-87 4.1387916446386643E-4 88-89 4.8416053201433425E-4 90-91 7.730950430551466E-4 92-93 2.4208026600716713E-4 94-95 3.5140683775233936E-4 96-97 6.2472326711527E-4 98-99 0.001116692839968545 100 1.561808167788175E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6402835.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.122852978887515 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66421186886302 40.78122879542933 2 15.987612857971364 17.94540891822597 3 5.180720445237126 8.722704356182797 4 2.21962466298386 4.982866745158236 5 1.1174263104663327 3.135657626852135 6 0.659737840529543 2.2215821897188968 7 0.4113791883215591 1.6161441593321457 8 0.310540581732827 1.3942738730055722 9 0.226437011430474 1.143746199134205 >10 1.1318240917061062 11.22579199561672 >50 0.06239030343393771 2.385693129981745 >100 0.026203629535129957 2.6039257844874197 >500 0.0013906148475118555 0.5133765968883482 >1k 3.893500654337496E-4 0.3849442697247089 >5k 2.7810718921145427E-5 0.09279056406582986 >10k+ 8.343215676343628E-5 0.8498647961958681 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21957 0.34292621940124957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20312 0.3172344750411341 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12181 0.1902438529182776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.1236163355763504E-5 0.0 2 0.0 0.0 0.0 3.1236163355763504E-5 0.0 3 0.0 0.0 0.0 6.247232671152701E-5 0.0 4 0.0 0.0 0.0 1.2494465342305402E-4 0.0 5 0.0 0.0 0.0 1.2494465342305402E-4 0.0 6 0.0 0.0 0.0 1.2494465342305402E-4 0.0 7 0.0 0.0 0.0 1.561808167788175E-4 0.0 8 0.0 0.0 0.0 1.561808167788175E-4 0.0 9 1.5618081677881752E-5 0.0 0.0 2.0303506181246276E-4 0.0 10-11 1.5618081677881752E-5 0.0 0.0 2.1084410265140364E-4 0.0 12-13 1.5618081677881752E-5 0.0 0.0 2.4208026600716715E-4 0.0 14-15 1.5618081677881752E-5 3.1236163355763504E-5 0.0 2.655073885239898E-4 0.0 16-17 1.5618081677881752E-5 6.247232671152701E-5 0.0 2.8112547020187154E-4 0.0 18-19 1.5618081677881752E-5 7.809040838940875E-5 0.0 3.0455259271869415E-4 0.0 20-21 1.5618081677881752E-5 7.809040838940875E-5 0.0 3.435977969133985E-4 0.0 22-23 1.5618081677881752E-5 7.809040838940875E-5 0.0 3.904520419470438E-4 0.0 24-25 1.5618081677881752E-5 7.809040838940875E-5 0.0 4.919695728532751E-4 0.0 26-27 1.5618081677881752E-5 7.809040838940875E-5 0.0 6.2472326711527E-4 0.0 28-29 1.5618081677881752E-5 9.37084900672905E-5 0.0 0.0011635470850021904 0.0 30-31 1.5618081677881752E-5 1.1713561258411314E-4 0.0 0.0028893451104081242 0.0 32-33 1.5618081677881752E-5 1.2494465342305402E-4 0.0 0.006551785263871394 0.0 34-35 1.5618081677881752E-5 1.327536942619949E-4 0.0 0.012799017935024096 0.0 36-37 1.5618081677881752E-5 1.7179889845669925E-4 0.0 0.021818460104000806 0.0 38-39 1.5618081677881752E-5 2.0303506181246276E-4 0.0 0.033352413423116484 0.0 40-41 1.5618081677881752E-5 2.4208026600716715E-4 0.0 0.04517530125327296 0.0 42-43 1.5618081677881752E-5 3.12361633557635E-4 0.0 0.05762291235054472 0.0 44-45 1.5618081677881752E-5 3.12361633557635E-4 0.0 0.07323318498758753 0.0 46-47 1.5618081677881752E-5 3.12361633557635E-4 0.0 0.0917406117758774 0.0 48-49 1.5618081677881752E-5 3.2017067439657585E-4 0.0 0.11043545554430186 0.0 50-51 1.5618081677881752E-5 4.216882053028073E-4 0.0 0.12879451055665186 0.0 52-53 1.5618081677881752E-5 4.216882053028073E-4 0.0 0.1468958672213168 0.0 54-55 1.5618081677881752E-5 4.3730628698068904E-4 0.0 0.1686909002028008 0.0 56-57 1.5618081677881752E-5 4.529243686585708E-4 0.0 0.19360174047902218 0.0 58-59 1.5618081677881752E-5 4.529243686585708E-4 0.0 0.2216127699683031 0.0 60-61 1.5618081677881752E-5 4.8416053201433425E-4 0.0 0.2515604415856414 0.0 62-63 1.5618081677881752E-5 4.997786136922161E-4 0.0 0.282382725776941 0.0 64-65 1.5618081677881752E-5 4.997786136922161E-4 0.0 0.3161021641194877 0.0 66-67 1.5618081677881752E-5 4.997786136922161E-4 0.0 0.3559360814389251 0.0 68-69 1.5618081677881752E-5 4.997786136922161E-4 0.0 0.40107233748800336 0.0 70-71 1.5618081677881752E-5 5.153966953700978E-4 0.0 0.4505972744885664 0.0 72-73 1.5618081677881752E-5 5.153966953700978E-4 0.0 0.5006376081844995 0.0 74-75 1.5618081677881752E-5 5.153966953700978E-4 0.0 0.5552852759754078 0.0 76-77 2.3427122516822626E-5 5.153966953700978E-4 0.0 0.6163207391725696 0.0 78-79 3.1236163355763504E-5 5.153966953700978E-4 0.0 0.6868129508256889 0.0 80-81 3.1236163355763504E-5 5.153966953700978E-4 0.0 0.7649736405826482 0.0 82-83 3.1236163355763504E-5 5.153966953700978E-4 0.0 0.8492019550714645 0.0 84-85 3.1236163355763504E-5 5.153966953700978E-4 0.0 0.9355387105867947 0.0 86-87 3.1236163355763504E-5 5.153966953700978E-4 0.0 1.030324848289859 0.0 88 3.1236163355763504E-5 5.153966953700978E-4 0.0 1.1089931257013494 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11965 0.0 60.498905 1 GTATCAA 22125 0.0 40.71576 1 GTGGTAT 4925 0.0 33.256527 1 ATCAACG 27845 0.0 31.703148 3 TCAACGC 28215 0.0 31.354046 4 TATCAAC 28935 0.0 30.752552 2 TGGTATC 4945 0.0 30.60861 2 CAACGCA 29290 0.0 30.219341 5 AACGCAG 30320 0.0 29.281208 6 ACGCAGA 34005 0.0 25.983717 7 CGCAGAG 34420 0.0 25.574852 8 GCAGAGT 37735 0.0 23.004284 9 GTACATG 18020 0.0 21.46945 1 GAGTACT 28510 0.0 21.46347 12-13 CAGAGTA 36685 0.0 21.222136 10-11 AGAGTAC 35425 0.0 20.696692 10-11 TACATGG 18165 0.0 20.054893 2 GTACTTT 31090 0.0 19.553833 14-15 AGTACTT 29945 0.0 19.218554 12-13 ACATGGG 18445 0.0 19.03689 3 >>END_MODULE