FastQCFastQC Report
Wed 25 May 2016
SRR2049437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402531
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64250.044.7217451
GTATCAA106700.034.686541
TCAACGC116300.030.349364
ATCAACG116400.030.2026273
CAACGCA130150.027.0481225
TATCAAC133600.027.0176432
AACGCAG136250.025.8714546
ACGCAGA148350.023.4444627
GTACATG178000.023.1438051
CGCAGAG148650.023.047378
TACATGG177100.021.9206872
GTGGTAT37400.020.8731081
ACATGGG182050.020.6535843
GCAGAGT174700.018.8042769
AGAGTAC154350.016.88388410-11
CAGAGTA170450.016.70909910-11
TGGTATC42350.016.6467912
CATGGGG128700.016.5427974
GAGTACA132050.015.8835331
GAGTACT76450.014.87606712-13
Produced by FastQC (version 0.11.5)