##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049437_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6402531 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98416813600746 31.0 31.0 34.0 28.0 34.0 2 31.207873105182934 31.0 31.0 34.0 28.0 34.0 3 31.276201864543882 33.0 31.0 34.0 28.0 34.0 4 34.86698853937607 37.0 35.0 37.0 32.0 37.0 5 34.88574971366792 37.0 35.0 37.0 32.0 37.0 6 34.88149171007528 37.0 35.0 37.0 32.0 37.0 7 34.803200171932005 37.0 35.0 37.0 32.0 37.0 8 34.78012585960146 37.0 35.0 37.0 32.0 37.0 9 36.34453843331645 39.0 37.0 39.0 32.0 39.0 10-11 36.28926443308123 39.0 37.0 39.0 32.0 39.0 12-13 36.21890100961635 39.0 37.0 39.0 32.0 39.0 14-15 37.45394430733721 40.0 38.0 41.0 32.0 41.0 16-17 37.441712660196416 40.0 38.0 41.0 32.0 41.0 18-19 37.45402911754742 40.0 38.0 41.0 32.0 41.0 20-21 37.51163758519873 40.0 38.0 41.0 32.0 41.0 22-23 37.46810628484266 40.0 38.0 41.0 32.0 41.0 24-25 37.47196507131321 40.0 38.0 41.0 32.0 41.0 26-27 37.474552329383485 40.0 38.0 41.0 32.0 41.0 28-29 37.45192768297412 40.0 38.0 41.0 32.0 41.0 30-31 37.38995804940265 40.0 38.0 41.0 32.0 41.0 32-33 37.34395624168005 40.0 38.0 41.0 31.5 41.0 34-35 37.2704321150495 40.0 37.5 41.0 31.0 41.0 36-37 37.14760100341568 40.0 37.0 41.0 31.0 41.0 38-39 37.033066688782924 40.0 37.0 41.0 30.5 41.0 40-41 36.90347934277866 40.0 37.0 41.0 30.0 41.0 42-43 36.799451888635915 40.0 37.0 41.0 30.0 41.0 44-45 36.63157913643839 40.0 36.5 41.0 30.0 41.0 46-47 36.50504300564886 40.0 36.0 41.0 30.0 41.0 48-49 36.397500847711626 40.0 36.0 41.0 30.0 41.0 50-51 36.277998107311 40.0 36.0 41.0 30.0 41.0 52-53 36.15555934051706 39.0 35.0 41.0 29.5 41.0 54-55 36.004122276018656 39.0 35.0 41.0 29.0 41.0 56-57 35.83068578660533 39.0 35.0 41.0 29.0 41.0 58-59 35.63841432396032 39.0 35.0 41.0 28.0 41.0 60-61 35.43466724331362 39.0 35.0 40.5 28.0 41.0 62-63 35.17170514285679 38.0 34.5 40.0 28.0 41.0 64-65 34.928396676251936 38.0 34.0 40.0 28.0 41.0 66-67 34.694820298410114 37.0 34.0 40.0 27.5 41.0 68-69 34.40302085222235 37.0 34.0 40.0 27.0 41.0 70-71 34.08760722907863 36.0 34.0 39.0 26.0 41.0 72-73 33.75137441739837 36.0 34.0 39.0 26.0 41.0 74-75 33.44894620580517 35.5 33.5 39.0 26.0 40.5 76-77 33.12119464942848 35.0 33.0 37.5 26.0 40.0 78-79 32.77083882920677 35.0 33.0 37.0 25.5 39.0 80-81 32.43274331666649 35.0 33.0 37.0 25.0 39.0 82-83 32.12834822666224 35.0 33.0 36.0 25.0 39.0 84-85 31.836855456068857 35.0 33.0 36.0 24.0 37.0 86-87 31.556206287794623 35.0 32.5 35.5 23.5 37.0 88-89 31.312467756891767 35.0 32.0 35.0 23.0 37.0 90-91 31.083468631389678 35.0 32.0 35.0 20.5 36.0 92-93 30.859392168503362 35.0 32.0 35.0 19.5 36.0 94-95 30.63627126522308 35.0 32.0 35.0 18.0 36.0 96-97 30.425612777197017 35.0 32.0 35.0 11.0 35.5 98-99 30.219999325266834 35.0 32.0 35.0 2.0 35.0 100 30.06122484998511 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3331609896395733 1101 2 0.38816228320079205 1101 3 0.35463998909488126 1101 4 0.36536200881817393 1101 5 0.3864468974889448 1101 6 0.4198231358874338 1101 7 0.4438130140773069 1101 8 0.3752486746380015 1101 9 0.5088417723327581 1101 10-11 0.5722111354226413 1101 12-13 0.5947454228063407 1101 14-15 0.6893864445354012 1101 16-17 0.579795758315079 1101 18-19 0.6041063309837682 1101 20-21 0.6200362025160899 1101 22-23 0.5745953701530624 1101 24-25 0.6230690967062529 1101 26-27 0.611625542955295 1101 28-29 0.6020056745927036 1101 30-31 0.6263960303865233 1101 32-33 0.6075411475502577 1101 34-35 0.5652421195290387 1101 36-37 0.5944589575886781 1101 38-39 0.5838783386013731 1101 40-41 0.5771357157700905 1101 42-43 0.5372414862929631 1101 44-45 0.5866752964877193 1101 46-47 0.5669148400462589 1101 48-49 0.5088885603757802 1101 50-51 0.5214867054703944 1101 52-53 0.5245932944061948 1101 54-55 0.49598411544643994 1101 56-57 0.47968771006068067 1101 58-59 0.4780110678967304 1101 60-61 0.49622217172478855 1101 62-63 0.4541225274093392 1101 64-65 0.45714760506034224 1101 66-67 0.4251773770883318 1101 68-69 0.46544015448671416 1101 70-71 0.5403166086651723 1101 72-73 0.5225748831183736 1101 74-75 0.4712624639154299 1101 76-77 0.4174350441855097 1101 78-79 0.3935229813068375 1101 80-81 0.4159436787224777 1101 82-83 0.3778625369565347 1101 84-85 0.3921356729547867 1101 86-87 0.3992525272258476 1101 88-89 0.37420405728630257 1101 90-91 0.4020102280641993 1101 92-93 0.32391240877339555 1101 94-95 0.3836328421245767 1101 96-97 0.40784763506258415 1101 98-99 0.4301463240209955 1101 100 0.37625260128012883 1102 1 0.2254161243821038 1102 2 0.3069289429844133 1102 3 0.35034513493701525 1102 4 0.3222630274904148 1102 5 0.32730493551103734 1102 6 0.3174472812042026 1102 7 0.30588623989858377 1102 8 0.3764825712184958 1102 9 0.46406251602272874 1102 10-11 0.4755375907853576 1102 12-13 0.44578506250383754 1102 14-15 0.5671842294311915 1102 16-17 0.4783925720371798 1102 18-19 0.5064858394014706 1102 20-21 0.5022492694884875 1102 22-23 0.5282646053212545 1102 24-25 0.4836417517699161 1102 26-27 0.45407378586885017 1102 28-29 0.5135462994145144 1102 30-31 0.4196972611414438 1102 32-33 0.4141359445216395 1102 34-35 0.4562303772014715 1102 36-37 0.47355884902847123 1102 38-39 0.5556062878986125 1102 40-41 0.5284291862144244 1102 42-43 0.48649283748272154 1102 44-45 0.3832485156658336 1102 46-47 0.3822500411206846 1102 48-49 0.338205371540262 1102 50-51 0.3732305471286068 1102 52-53 0.3532478869566731 1102 54-55 0.38187933021682596 1102 56-57 0.4082182624776607 1102 58-59 0.39822781047065803 1102 60-61 0.3784621642290986 1102 62-63 0.3515075537964947 1102 64-65 0.3772832388808496 1102 66-67 0.3529555024014641 1102 68-69 0.30941394226342567 1102 70-71 0.2615759665157995 1102 72-73 0.3165745653541947 1102 74-75 0.3996869265535068 1102 76-77 0.3438901370208072 1102 78-79 0.37947168730355685 1102 80-81 0.3700304568538826 1102 82-83 0.342259745503398 1102 84-85 0.3157154220760887 1102 86-87 0.28238576093032464 1102 88-89 0.2833066170594627 1102 90-91 0.3079121856354803 1102 92-93 0.26394794134117916 1102 94-95 0.34502484714458603 1102 96-97 0.36390864772277354 1102 98-99 0.303260042077806 1102 100 0.2693798852101743 1103 1 0.2623206348189271 1103 2 0.32897616846544864 1103 3 0.32287143160531073 1103 4 0.36728306095623253 1103 5 0.32962971424620946 1103 6 0.33785491810835566 1103 7 0.3859156157560335 1103 8 0.34437272790932383 1103 9 0.4149269571508043 1103 10-11 0.41995067418795173 1103 12-13 0.4244329186202478 1103 14-15 0.5401055497679579 1103 16-17 0.4610303032289309 1103 18-19 0.45860809785342127 1103 20-21 0.4539121438865621 1103 22-23 0.4651430863501247 1103 24-25 0.45261262412287095 1103 26-27 0.4097533175032666 1103 28-29 0.4036255953447849 1103 30-31 0.4401592283268272 1103 32-33 0.46066310844068425 1103 34-35 0.4835799225023081 1103 36-37 0.4749101992198277 1103 38-39 0.5108721619251213 1103 40-41 0.5344973266086797 1103 42-43 0.46181159042976816 1103 44-45 0.42823507803350935 1103 46-47 0.43432584018290044 1103 48-49 0.4044796579782499 1103 50-51 0.41664576392577857 1103 52-53 0.4591674774801433 1103 54-55 0.3723258111590724 1103 56-57 0.35753462734867725 1103 58-59 0.402615799463824 1103 60-61 0.38708866791108676 1103 62-63 0.37235139128134165 1103 64-65 0.4585266073081087 1103 66-67 0.3803002274415803 1103 68-69 0.36717212879946715 1103 70-71 0.3880879618006574 1103 72-73 0.39120792344693456 1103 74-75 0.40139522695664454 1103 76-77 0.36410401264976144 1103 78-79 0.33077499666347165 1103 80-81 0.3089485024507681 1103 82-83 0.2994289291935601 1103 84-85 0.2999468040996405 1103 86-87 0.33239724041427365 1103 88-89 0.3362197086168841 1103 90-91 0.2910653479675389 1103 92-93 0.24355781584998226 1103 94-95 0.27701582150350745 1103 96-97 0.27494444317705913 1103 98-99 0.3221282990035128 1103 100 0.27865287829957097 1104 1 0.20164726584740933 1104 2 0.1597574516944782 1104 3 0.2070958103758116 1104 4 0.2728925302187548 1104 5 0.25940746940426607 1104 6 0.24699645719069707 1104 7 0.30136987534280735 1104 8 0.27353429756463754 1104 9 0.3741823724034887 1104 10-11 0.4238395499493208 1104 12-13 0.3881072382260271 1104 14-15 0.4067615528507105 1104 16-17 0.4163155934813858 1104 18-19 0.4105685311099876 1104 20-21 0.41542545185753 1104 22-23 0.3910492334821356 1104 24-25 0.38134468158262536 1104 26-27 0.38687774880403936 1104 28-29 0.3624713108561153 1104 30-31 0.40335368665614624 1104 32-33 0.3512749133916131 1104 34-35 0.41862833577920355 1104 36-37 0.42742320000687073 1104 38-39 0.404393671161543 1104 40-41 0.4047068478561755 1104 42-43 0.3979571410796652 1104 44-45 0.3603539745706925 1104 46-47 0.3698886502069101 1104 48-49 0.3691700474838271 1104 50-51 0.3596580567356753 1104 52-53 0.3096877816103998 1104 54-55 0.31776140341512615 1104 56-57 0.3046439175720508 1104 58-59 0.3606915557451842 1104 60-61 0.34835477461842146 1104 62-63 0.36990009992739203 1104 64-65 0.3506445223108585 1104 66-67 0.3184277505757933 1104 68-69 0.32705627138369664 1104 70-71 0.38483272104299004 1104 72-73 0.400794061272542 1104 74-75 0.4149264061248559 1104 76-77 0.3414065742728525 1104 78-79 0.292090907154801 1104 80-81 0.3746987673741913 1104 82-83 0.3624487107476213 1104 84-85 0.32428776734844433 1104 86-87 0.3828130293043941 1104 88-89 0.3113696198243723 1104 90-91 0.28493693781701523 1104 92-93 0.3162847911425821 1104 94-95 0.3465130032273329 1104 96-97 0.42147808163430156 1104 98-99 0.4481589555955168 1104 100 0.40685218068306384 1105 1 0.1828559631066362 1105 2 0.21845379612928895 1105 3 0.236623575059987 1105 4 0.23699635273034403 1105 5 0.20099478952710115 1105 6 0.2976537986751282 1105 7 0.244595661905322 1105 8 0.301430739792103 1105 9 0.2849614839252155 1105 10-11 0.3210244186591993 1105 12-13 0.31803303537011374 1105 14-15 0.39329028341349215 1105 16-17 0.3275177377708758 1105 18-19 0.325689319576135 1105 20-21 0.3443246162694322 1105 22-23 0.37130446329238964 1105 24-25 0.38335221344754444 1105 26-27 0.44103570853965124 1105 28-29 0.46523715471797544 1105 30-31 0.4552998692646142 1105 32-33 0.4408958649161505 1105 34-35 0.4304209871707698 1105 36-37 0.42213040465194496 1105 38-39 0.461983109923338 1105 40-41 0.4550322968436973 1105 42-43 0.38694529070126293 1105 44-45 0.3584564539892696 1105 46-47 0.3129648732480135 1105 48-49 0.2843424966335917 1105 50-51 0.33931072790783645 1105 52-53 0.34684080970927056 1105 54-55 0.27864939831863467 1105 56-57 0.2876705768701626 1105 58-59 0.2732406193185568 1105 60-61 0.25323881064138476 1105 62-63 0.33178462596704605 1105 64-65 0.3192402754696815 1105 66-67 0.31704939171262936 1105 68-69 0.2603954938614095 1105 70-71 0.2579456969704452 1105 72-73 0.27353380041521547 1105 74-75 0.29458589193026086 1105 76-77 0.24381041314417473 1105 78-79 0.257965147893195 1105 80-81 0.3046841380711669 1105 82-83 0.2577612444431523 1105 84-85 0.25897050058257065 1105 86-87 0.268379511373368 1105 88-89 0.26025787258933875 1105 90-91 0.37535303886286897 1105 92-93 0.37992825296997523 1105 94-95 0.32722743373268415 1105 96-97 0.2819958341716493 1105 98-99 0.2695808435945004 1105 100 0.23666101387412297 1106 1 0.1873318018221859 1106 2 0.19485001637820787 1106 3 0.22313775466388108 1106 4 0.2718239611513127 1106 5 0.2601907244616868 1106 6 0.23198919666777584 1106 7 0.3031919307415052 1106 8 0.20233937207508035 1106 9 0.29489712810475055 1106 10-11 0.27846841573627756 1106 12-13 0.2890669038603164 1106 14-15 0.3646335478692322 1106 16-17 0.2714159130888305 1106 18-19 0.2785252884302878 1106 20-21 0.27148743893141614 1106 22-23 0.2789793946626773 1106 24-25 0.33037656461756626 1106 26-27 0.34457724164067827 1106 28-29 0.30919383585381865 1106 30-31 0.3598668528250428 1106 32-33 0.2946743793634923 1106 34-35 0.267612064282865 1106 36-37 0.2639418184044118 1106 38-39 0.2987907146695079 1106 40-41 0.3750185301828637 1106 42-43 0.38177874993571237 1106 44-45 0.3291275688002244 1106 46-47 0.3672663500530646 1106 48-49 0.2999143939575575 1106 50-51 0.284889960816912 1106 52-53 0.2600191559906193 1106 54-55 0.2947742071312618 1106 56-57 0.21244854952703207 1106 58-59 0.23010642196945952 1106 60-61 0.2562353397603374 1106 62-63 0.3334133626476401 1106 64-65 0.31668023469076445 1106 66-67 0.3107450492531427 1106 68-69 0.32546479129427297 1106 70-71 0.33290046078500524 1106 72-73 0.30137375516935805 1106 74-75 0.28186203497707396 1106 76-77 0.2423857818820565 1106 78-79 0.2259220799933317 1106 80-81 0.23358960674911344 1106 82-83 0.2815507683499803 1106 84-85 0.2883203361461639 1106 86-87 0.24394038684254582 1106 88-89 0.25233217270752206 1106 90-91 0.2518771148977734 1106 92-93 0.1442984809458423 1106 94-95 0.21401125629945028 1106 96-97 0.31197647468176726 1106 98-99 0.2943048341389023 1106 100 0.3095555397491907 1107 1 0.1399837141073057 1107 2 0.18184781137428274 1107 3 0.18500880815041043 1107 4 0.18384213027820806 1107 5 0.1628659519277278 1107 6 0.17573806487161647 1107 7 0.21456327375850748 1107 8 0.1691476016189668 1107 9 0.21374092596257555 1107 10-11 0.21804092633659167 1107 12-13 0.21388429818263432 1107 14-15 0.2939543902010371 1107 16-17 0.2708228017797012 1107 18-19 0.2783871662392414 1107 20-21 0.3022283324561599 1107 22-23 0.3131921118530414 1107 24-25 0.2895787030547936 1107 26-27 0.327802667058414 1107 28-29 0.34789833210994914 1107 30-31 0.32751038933398036 1107 32-33 0.3182464708787123 1107 34-35 0.2890499242665996 1107 36-37 0.26170074985658687 1107 38-39 0.2343450657572248 1107 40-41 0.30981102717721143 1107 42-43 0.2542968190486192 1107 44-45 0.26374148166577527 1107 46-47 0.22035990887477652 1107 48-49 0.24173054282088202 1107 50-51 0.2411027063849076 1107 52-53 0.22909867038843856 1107 54-55 0.20610429625086368 1107 56-57 0.2424795204654444 1107 58-59 0.22542043557115932 1107 60-61 0.29620605763431485 1107 62-63 0.2862409902847034 1107 64-65 0.2867623589112611 1107 66-67 0.307576064306474 1107 68-69 0.30346994000662875 1107 70-71 0.2691111027309816 1107 72-73 0.26087339352071126 1107 74-75 0.3256177716308528 1107 76-77 0.3090010900586435 1107 78-79 0.27500272617346866 1107 80-81 0.29553706052633544 1107 82-83 0.2605131359676989 1107 84-85 0.24485720365605346 1107 86-87 0.2533965455022553 1107 88-89 0.2172727343646308 1107 90-91 0.2876764816822899 1107 92-93 0.2109070325437159 1107 94-95 0.19556889225851037 1107 96-97 0.20884354771270708 1107 98-99 0.2508324861184761 1107 100 0.2154698932023713 1108 1 0.16768112086475995 1108 2 0.14039601321538697 1108 3 0.19118624266253192 1108 4 0.18178916062981898 1108 5 0.20046443215758103 1108 6 0.18027346049351678 1108 7 0.15889731330890555 1108 8 0.15222196998134052 1108 9 0.22039160450357542 1108 10-11 0.2004239542226287 1108 12-13 0.18891772841029564 1108 14-15 0.20777850509996654 1108 16-17 0.18710913294273723 1108 18-19 0.2406093248383101 1108 20-21 0.2697968012675247 1108 22-23 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-0.6233228566410816 2215 42-43 -0.6536532141106335 2215 44-45 -0.6116900279818012 2215 46-47 -0.5236352111510314 2215 48-49 -0.5615695447422269 2215 50-51 -0.5727332264687632 2215 52-53 -0.5720473391121743 2215 54-55 -0.5715965807547931 2215 56-57 -0.5160138793919344 2215 58-59 -0.5817507376092905 2215 60-61 -0.5465989037709278 2215 62-63 -0.5343912528202281 2215 64-65 -0.5905115226098943 2215 66-67 -0.5680120299494291 2215 68-69 -0.5992534897523356 2215 70-71 -0.5399412080427695 2215 72-73 -0.49220961684924447 2215 74-75 -0.5525184337957327 2215 76-77 -0.5781419747464298 2215 78-79 -0.47392263027743553 2215 80-81 -0.5853197036930311 2215 82-83 -0.5291029595916399 2215 84-85 -0.5005201795642122 2215 86-87 -0.5478334797212483 2215 88-89 -0.5427489289219878 2215 90-91 -0.4846443094754278 2215 92-93 -0.5240573048906683 2215 94-95 -0.5441831628303717 2215 96-97 -0.5794166688222653 2215 98-99 -0.5647244776163376 2215 100 -0.5447541736509862 2216 1 -0.5004062311671014 2216 2 -0.6864636732698095 2216 3 -0.6726887524243708 2216 4 -0.7116805288068804 2216 5 -0.694434793703806 2216 6 -0.7114490535079057 2216 7 -0.6378095963818851 2216 8 -0.6450909127080919 2216 9 -0.7383413438639934 2216 10-11 -0.799830582886635 2216 12-13 -0.8003742578421367 2216 14-15 -0.9693129654009951 2216 16-17 -0.9065508960445996 2216 18-19 -1.0257245462251134 2216 20-21 -0.9568274137308492 2216 22-23 -0.9923022608818002 2216 24-25 -1.0079197354045206 2216 26-27 -1.0750146002755514 2216 28-29 -0.9832927536100371 2216 30-31 -1.0648077065918216 2216 32-33 -1.041401730027367 2216 34-35 -1.004778989326482 2216 36-37 -1.0151406303902135 2216 38-39 -0.9242650400967278 2216 40-41 -0.937543927855863 2216 42-43 -0.9087508730747587 2216 44-45 -0.9273806417841186 2216 46-47 -0.8675585645144821 2216 48-49 -1.013947835642604 2216 50-51 -0.876981271684862 2216 52-53 -1.0207938210671372 2216 54-55 -0.9606085445779371 2216 56-57 -0.9247538554398531 2216 58-59 -0.9066160340534921 2216 60-61 -0.9042011430530081 2216 62-63 -0.9397463978866369 2216 64-65 -0.9078103178501493 2216 66-67 -0.9354168739125157 2216 68-69 -0.8696502026646442 2216 70-71 -0.8627006852451728 2216 72-73 -0.8608557199566249 2216 74-75 -0.9109016371242689 2216 76-77 -0.9248815462655884 2216 78-79 -0.9634772370471616 2216 80-81 -0.9505293582691898 2216 82-83 -0.9099395796319953 2216 84-85 -0.7977659915745647 2216 86-87 -0.9588543191681715 2216 88-89 -0.9236298241455181 2216 90-91 -0.966866504656835 2216 92-93 -0.9988995591352037 2216 94-95 -0.9589558803013958 2216 96-97 -0.9952814920331186 2216 98-99 -1.0371002242084835 2216 100 -1.1215472868476262 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91422.0 3 16824.0 4 10590.0 5 8969.0 6 7880.0 7 8585.0 8 9791.0 9 11105.0 10 12236.0 11 13791.0 12 15472.0 13 17233.0 14 19512.0 15 20452.0 16 20177.0 17 20869.0 18 20026.0 19 18431.0 20 18385.0 21 20079.0 22 22193.0 23 26609.0 24 32272.0 25 39901.0 26 48678.0 27 57166.0 28 69150.0 29 87241.0 30 106240.0 31 125678.0 32 159849.0 33 213343.0 34 295941.0 35 434304.0 36 727165.0 37 1279520.0 38 1678649.0 39 615543.0 40 1260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.600105293532295 20.524032279595595 14.740594314461886 23.135268112410223 2 15.479028661708979 20.484213269033162 40.305721563311415 23.73103650594644 3 18.230418513013916 25.462642574770623 30.4761099875683 25.830828924647165 4 12.77966864719813 17.49111228890173 38.864091241595936 30.8651278223042 5 13.293233942410456 37.61819489947244 35.50036348436392 13.58820767375318 6 30.266491931515898 38.04230506695491 18.114500466255446 13.576702535273746 7 26.560890698947787 32.596650818236704 22.834642840126133 18.007815642689376 8 24.53692697143632 36.05226318537011 20.916270873080613 18.494538970112963 9 24.6452372751761 17.367741572691468 22.018320966017413 35.96870018611502 10-11 23.442356627703 27.171942096540132 29.281822456257643 20.103878819499226 12-13 24.04310473905157 24.884138908495768 28.972884345421836 22.099872007030825 14-15 21.624956379850975 27.13916803527976 26.260852708698007 24.975022876171263 16-17 21.160390683358468 30.267463079372902 26.945653236809065 21.626493000459565 18-19 21.095043479761756 29.225446135868605 28.82126003875507 20.85825034561457 20-21 22.183772242893447 28.51316595902685 28.28708466270453 21.015977135375177 22-23 21.924701440859977 28.384835315448502 28.269818326894118 21.4206449167974 24-25 21.712786376553094 28.501069984767618 28.239045172743293 21.547098465935992 26-27 21.460520882819992 28.464344653394697 28.355756785355506 21.719377678429804 28-29 21.459474750857655 28.44132599743633 28.567392852155393 21.531806399550625 30-31 21.771006086212306 28.794526017257205 28.058012008097037 21.376455888433448 32-33 21.382566086319333 28.601797542262332 28.43185939574875 21.58377697566959 34-35 21.704022054437246 28.289354991434678 28.37902260504841 21.627600349079668 36-37 21.929782016325365 28.5425910535478 27.9615584706895 21.566068459437336 38-39 21.701336395634907 28.71057696145563 28.07315811800614 21.51492852490332 40-41 21.666513972385847 28.392458823897883 28.241240717964605 21.69978648575167 42-43 21.597484606814003 28.439230361787317 28.218081446468236 21.745203584930444 44-45 21.668321440597676 28.577676306847653 28.0667780368187 21.687224215735966 46-47 21.750071801658862 28.58434779294159 27.96831025877576 21.69727014662379 48-49 21.59674314306752 28.442963098697444 28.053594326791547 21.906699431443485 50-51 21.611322719643944 28.406136969405672 28.011355085088553 21.971185225861834 52-53 21.60683369771942 28.433666228591097 28.02202046326042 21.937479610429055 54-55 21.70640398428617 28.68397370781016 27.8527555516469 21.75686675625677 56-57 21.602541260410298 28.451784210948112 27.91796517952088 22.02770934912071 58-59 21.56358889847384 28.17058886832889 28.128518700568332 22.13730353262894 60-61 21.693902235298538 28.44800877465441 27.83318472267314 22.024904267373916 62-63 21.564982801013507 28.503157052834162 27.908440781436965 22.023419364715366 64-65 21.630225514657113 28.230853407153784 27.970446199552207 22.1684748786369 66-67 21.457889474270402 28.28551514258762 27.92621287458529 22.330382508556696 68-69 21.72052334692029 28.409369869694384 27.896342237325033 21.97376454606029 70-71 21.701414425245574 28.183628518536196 27.882809965553896 22.232147090664338 72-73 21.52871135610602 28.258505036890224 28.043178946393276 22.169604660610478 74-75 21.574125337514207 28.27884781819443 27.878720467311503 22.26830637697986 76-77 21.631126132254618 28.253416660171236 27.70496563619241 22.410491571381733 78-79 21.6462734566694 28.21924882423767 27.938765385506958 22.19571233358597 80-81 21.477355691748624 28.153152291136387 27.960042732021844 22.40944928509315 82-83 21.41723576417904 28.19628664410141 28.093314470722326 22.293163120997225 84-85 21.426819133005658 27.994168521026058 28.007399161471064 22.57161318449722 86-87 21.59408788810634 28.137636326854835 27.885169497151814 22.383106287887014 88-89 21.437442045304014 28.054634251194084 27.92069798073967 22.58722572276223 90-91 21.374185856658283 27.909296144630787 27.983598879889975 22.732919118820956 92-93 21.487699414209473 28.000709149789536 27.87919305314367 22.632398382857318 94-95 21.36950813369863 27.811014104169985 28.034413246861522 22.785064515269866 96-97 21.433985188568556 27.867721223673108 27.923425377444065 22.774868210314267 98-99 21.435619024069418 27.841823639448382 27.880585693623257 22.841971642858944 100 21.47879623971739 27.714371222316775 27.86946163679509 22.937370901170745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2353.0 1 1733.0 2 1269.5 3 1486.0 4 1481.0 5 1321.5 6 1217.0 7 1230.5 8 1289.0 9 1339.5 10 1264.0 11 1135.0 12 1132.0 13 1208.0 14 1322.5 15 1510.5 16 1737.0 17 2176.0 18 2871.0 19 3836.0 20 5349.0 21 7671.5 22 11253.0 23 16665.5 24 24015.5 25 33776.0 26 46800.5 27 61721.0 28 79710.0 29 100657.5 30 118501.5 31 134946.0 32 151826.5 33 166303.0 34 179457.0 35 190237.0 36 199900.5 37 210219.0 38 219535.5 39 223931.5 40 227260.5 41 229325.5 42 226469.5 43 223488.0 44 220036.5 45 217313.0 46 214028.0 47 208340.5 48 203447.5 49 199724.5 50 194571.5 51 184721.0 52 172162.0 53 162323.5 54 156001.0 55 150114.0 56 138582.0 57 123221.0 58 111118.5 59 101807.0 60 90790.0 61 77486.0 62 65893.5 63 55996.0 64 46467.5 65 38273.5 66 31177.5 67 25132.0 68 20373.0 69 16249.0 70 12673.0 71 9906.5 72 7780.5 73 6254.0 74 5038.5 75 3815.0 76 2806.0 77 2144.5 78 1673.5 79 1364.5 80 971.0 81 526.0 82 338.0 83 234.0 84 182.0 85 143.0 86 104.0 87 86.0 88 73.0 89 60.5 90 45.5 91 35.0 92 26.5 93 21.5 94 18.0 95 13.0 96 11.0 97 11.5 98 11.0 99 12.0 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08085864793157581 2 0.013885133863467432 3 0.018008503199750225 4 0.011151839795855732 5 0.009449388062314731 6 0.007200277515251391 7 0.019773430226265206 8 0.009761764527184639 9 0.008996442188253363 10-11 0.013135430347779652 12-13 0.01573596441782164 14-15 0.01051146804287242 16-17 0.012885529175883724 18-19 0.008270166907430827 20-21 0.013299427991836355 22-23 0.013557138575354026 24-25 0.01621233852674825 26-27 0.005349446960897183 28-29 0.012010875074247982 30-31 0.01068327509855087 32-33 0.012338870362361384 34-35 0.008652828076896464 36-37 0.010191282166380764 38-39 0.01945324434977355 40-41 0.013283809168592857 42-43 0.007965599854182666 44-45 0.02115569608331455 46-47 0.019000298475712184 48-49 0.017875743202180515 50-51 0.010019475110702315 52-53 0.010425564515033195 54-55 0.01221391977641342 56-57 0.02027323257005706 58-59 0.0020616846681413958 60-61 0.007114373987412165 62-63 0.015478253834303964 64-65 0.024904213661753456 66-67 0.012776197413179256 68-69 0.01447083973509851 70-71 0.025966293642311143 72-73 0.02045284903735726 74-75 0.020601227858170465 76-77 0.009246343360149291 78-79 0.011354884498021172 80-81 0.019406387880043063 82-83 0.012518486829661581 84-85 0.011315837439912435 86-87 0.017118230274870985 88-89 0.01529082795538202 90-91 0.015884343238634847 92-93 0.0077859833868824686 94-95 0.015376731483221245 96-97 0.013338475049945092 98-99 0.010175663343137268 100 0.0071690398687644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6402531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.89128652111824 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52015974770508 45.22999525570425 2 14.569197214368385 17.451359294968288 3 4.585593412393057 8.239112667929547 4 1.9116063207414553 4.579542474844286 5 0.9864264742344562 2.953917530019613 6 0.560486303961126 2.0140947493019015 7 0.36310061130826654 1.5222593923499599 8 0.27351576671075983 1.3104968921693958 9 0.1908113232577238 1.0285142069431843 >10 0.9540154976100976 10.247204644476765 >50 0.05958436423824256 2.433222372650601 >100 0.02461464893319374 2.5309808725938665 >500 7.315606021961651E-4 0.3007173877069323 >1k 1.5675393577261817E-4 0.15858225834140227 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.5618823243495423E-5 0.0 1.5618823243495423E-5 0.0 3 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 4 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 5 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 6 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 7 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 8 0.0 1.5618823243495423E-5 0.0 3.1237646486990846E-5 0.0 9 0.0 1.5618823243495423E-5 0.0 9.371293946097254E-5 0.0 10-11 0.0 1.5618823243495423E-5 0.0 9.371293946097254E-5 0.0 12-13 0.0 1.5618823243495423E-5 0.0 1.0933176270446797E-4 0.0 14-15 0.0 1.5618823243495423E-5 0.0 1.3275999756971112E-4 0.0 16-17 0.0 1.5618823243495423E-5 0.0 2.1866352540893594E-4 0.0 18-19 0.0 3.904705810873856E-5 0.0 2.8894823000466535E-4 0.0 20-21 0.0 5.4665881352233985E-5 0.0 3.2018587649165623E-4 0.0 22-23 0.0 6.247529297398169E-5 0.0 4.373270508178719E-4 0.0 24-25 0.0 6.247529297398169E-5 0.0 8.199882202835098E-4 0.0 26-27 0.0 7.809411621747712E-5 0.0 0.0015384540894842992 0.0 28-29 0.0 7.809411621747712E-5 0.0 0.0038422305178998743 0.0 30-31 0.0 7.809411621747712E-5 0.0 0.009871096289889109 0.0 32-33 0.0 8.590352783922482E-5 0.0 0.0249120230733752 0.0 34-35 0.0 9.371293946097254E-5 0.0 0.05116726494569101 0.0 36-37 0.0 9.371293946097254E-5 0.0 0.09246343360149291 0.0 38-39 0.0 9.371293946097254E-5 0.0 0.14484115734855482 0.0 40-41 0.0 9.371293946097254E-5 0.0 0.1979685846113045 0.0 42-43 0.0 1.0933176270446797E-4 0.0 0.2537043553557179 0.0 44-45 0.0 1.0933176270446797E-4 0.0 0.3265739751982458 0.0 46-47 0.0 1.0933176270446797E-4 0.0 0.4181393264632377 0.0 48-49 0.0 1.0933176270446797E-4 0.0 0.5164207717229327 0.0 50-51 0.0 1.7180705567844967E-4 0.0 0.6112582664574369 0.0 52-53 0.0 1.7961646730019738E-4 0.0 0.7067751800030331 0.0 54-55 0.0 2.0304470216544053E-4 0.0 0.8196133685256659 0.0 56-57 0.0 2.2647293703068365E-4 0.0 0.958964509504132 0.0 58-59 0.0 2.3428234865243135E-4 0.0 1.1108419467238815 0.0 60-61 0.0 2.4209176027417906E-4 0.0 1.2678501673791192 0.0 62-63 0.0 2.4990117189592676E-4 0.0 1.4260297997776192 0.0 64-65 0.0 2.7332940676116994E-4 0.0 1.6017181330320776 0.0 66-67 0.0 2.8113881838291764E-4 0.0 1.805629679887532 0.0 68-69 0.0 2.9675764162641306E-4 0.0 2.0338128780633786 0.0 70-71 0.0 3.1237646486990847E-4 0.0 2.2823864499836084 0.0 72-73 0.0 3.5923293460039476E-4 0.0 2.535434814763099 0.0 74-75 0.0 3.748517578438902E-4 0.0 2.798869696999515 0.0 76-77 0.0 3.826611694656379E-4 0.0 3.0845535929462895 0.0 78-79 0.0 3.904705810873856E-4 0.0 3.4122130763599583 0.0 80-81 0.0 3.904705810873856E-4 0.0 3.7657919969462075 0.0 82-83 0.0 3.904705810873856E-4 0.0 4.1310381784953485 0.0 84-85 0.0 4.0608940433088105E-4 0.0 4.497565103550455 0.0 86-87 0.0 4.2170822757437647E-4 0.0 4.89022231989193 0.0 88 0.0 4.2170822757437647E-4 0.0 5.206565965865686 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6425 0.0 44.721745 1 GTATCAA 10670 0.0 34.68654 1 TCAACGC 11630 0.0 30.34936 4 ATCAACG 11640 0.0 30.202627 3 CAACGCA 13015 0.0 27.048122 5 TATCAAC 13360 0.0 27.017643 2 AACGCAG 13625 0.0 25.871454 6 ACGCAGA 14835 0.0 23.444462 7 GTACATG 17800 0.0 23.143805 1 CGCAGAG 14865 0.0 23.04737 8 TACATGG 17710 0.0 21.920687 2 GTGGTAT 3740 0.0 20.873108 1 ACATGGG 18205 0.0 20.653584 3 GCAGAGT 17470 0.0 18.804276 9 AGAGTAC 15435 0.0 16.883884 10-11 CAGAGTA 17045 0.0 16.709099 10-11 TGGTATC 4235 0.0 16.646791 2 CATGGGG 12870 0.0 16.542797 4 GAGTACA 13205 0.0 15.883533 1 GAGTACT 7645 0.0 14.876067 12-13 >>END_MODULE