FastQCFastQC Report
Wed 25 May 2016
SRR2049437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402531
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201610.31489109541211124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200040.3124389401628825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108170.16894881102488998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126400.060.5416451
GTATCAA229600.039.8273741
GTGGTAT48450.036.2322961
ATCAACG278100.032.5039633
TCAACGC279500.032.3411564
TGGTATC52150.031.8184092
TATCAAC290000.031.2674372
CAACGCA293050.030.829735
AACGCAG304450.029.7478736
ACGCAGA344350.026.1233377
CGCAGAG346000.025.9036798
GCAGAGT376450.023.5836759
GTACATG172100.022.9436151
GAGTACT281700.022.08960512-13
CAGAGTA367100.021.99508310-11
TACATGG174950.021.3336222
ACATGGG176400.020.492073
GTACTTT305450.020.27203414-15
AGAGTAC354900.019.97018810-11
AGTACTT292700.018.8187912-13
Produced by FastQC (version 0.11.5)