##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049437_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6402531
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960344901102392	33.0	31.0	34.0	30.0	34.0
2	32.06350613530805	33.0	31.0	34.0	30.0	34.0
3	32.08652671888664	34.0	31.0	34.0	30.0	34.0
4	35.798163413812446	37.0	35.0	37.0	33.0	37.0
5	35.85966784073361	37.0	35.0	37.0	35.0	37.0
6	35.93059197995293	37.0	35.0	37.0	35.0	37.0
7	35.90072332332323	37.0	35.0	37.0	35.0	37.0
8	35.901472870650686	37.0	35.0	37.0	35.0	37.0
9	37.60440785058284	39.0	37.0	39.0	35.0	39.0
10-11	37.592530672635554	39.0	37.0	39.0	35.0	39.0
12-13	37.578315513036955	39.0	37.0	39.0	35.0	39.0
14-15	38.864922012872725	40.0	38.0	41.0	35.0	41.0
16-17	38.87020047228198	40.0	38.0	41.0	35.0	41.0
18-19	38.958926009104836	40.0	38.5	41.0	35.0	41.0
20-21	38.988864091403855	40.0	39.0	41.0	35.0	41.0
22-23	38.95964291309171	40.0	38.5	41.0	35.0	41.0
24-25	38.94069431292094	40.0	38.0	41.0	35.0	41.0
26-27	38.835748706253824	40.0	38.0	41.0	35.0	41.0
28-29	38.7534731186776	40.0	38.0	41.0	35.0	41.0
30-31	38.63191330116168	40.0	38.0	41.0	35.0	41.0
32-33	38.485419281843384	40.0	38.0	41.0	34.0	41.0
34-35	38.364386287235476	40.0	38.0	41.0	34.0	41.0
36-37	38.22411433853269	40.0	38.0	41.0	34.0	41.0
38-39	38.1054810199279	40.0	38.0	41.0	34.0	41.0
40-41	37.99971433172288	40.0	38.0	41.0	33.0	41.0
42-43	37.84069128286923	40.0	37.5	41.0	33.0	41.0
44-45	37.64539945218539	40.0	37.0	41.0	33.0	41.0
46-47	37.371165871746655	40.0	37.0	41.0	32.5	41.0
48-49	37.09667465881852	40.0	37.0	41.0	32.0	41.0
50-51	36.92031432569401	40.0	37.0	41.0	31.0	41.0
52-53	36.79551110334335	40.0	36.0	41.0	31.0	41.0
54-55	36.64551846761851	40.0	36.0	41.0	31.0	41.0
56-57	36.488385686847906	39.0	35.5	41.0	31.0	41.0
58-59	36.305950959081656	39.0	35.0	41.0	30.5	41.0
60-61	36.11307653176533	39.0	35.0	41.0	30.0	41.0
62-63	35.894372475510075	39.0	35.0	41.0	30.0	41.0
64-65	35.67585920318074	38.0	35.0	40.5	30.0	41.0
66-67	35.413927164116814	37.5	35.0	40.0	29.0	41.0
68-69	35.12282392697513	37.0	35.0	40.0	29.0	41.0
70-71	34.84319091934112	37.0	34.5	39.5	29.0	41.0
72-73	34.51236885850299	36.0	34.0	39.0	29.0	41.0
74-75	34.19082187965978	36.0	34.0	39.0	28.5	41.0
76-77	33.87477241422181	35.0	34.0	38.5	28.0	40.0
78-79	33.57194357981243	35.0	34.0	37.0	28.0	39.0
80-81	33.26547962048134	35.0	34.0	37.0	28.0	39.0
82-83	32.999099887216474	35.0	34.0	36.5	28.0	39.0
84-85	32.722325983271304	35.0	34.0	36.0	27.0	37.0
86-87	32.4786835081314	35.0	33.5	36.0	27.0	37.0
88-89	32.26216772710667	35.0	33.0	35.0	27.0	37.0
90-91	32.073827522272055	35.0	33.0	35.0	26.0	36.0
92-93	31.9129336507703	35.0	33.0	35.0	26.0	36.0
94-95	31.75091920679494	35.0	33.0	35.0	26.0	36.0
96-97	31.60606243062314	35.0	33.0	35.0	25.5	36.0
98-99	31.46943911712415	35.0	33.0	35.0	25.0	35.0
100	31.356543997990794	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3033733462056425
1101	2	0.330623264697536
1101	3	0.4503399799350234
1101	4	0.2591638712455051
1101	5	0.2546247706462168
1101	6	0.24698853688475708
1101	7	0.2743274462092131
1101	8	0.282211785317557
1101	9	0.3651543389986571
1101	10-11	0.35348538465702717
1101	12-13	0.3236206350981661
1101	14-15	0.4210299344229824
1101	16-17	0.40675944221379723
1101	18-19	0.4101831018611293
1101	20-21	0.4083791290037908
1101	22-23	0.40776451950373627
1101	24-25	0.3953760096275616
1101	26-27	0.3783932538696533
1101	28-29	0.4403460660891909
1101	30-31	0.412687743938001
1101	32-33	0.4297224761591778
1101	34-35	0.46985678225757965
1101	36-37	0.4749182271888799
1101	38-39	0.4608490616623868
1101	40-41	0.5115741659131103
1101	42-43	0.48834917873817574
1101	44-45	0.4887176012735708
1101	46-47	0.5003650253491685
1101	48-49	0.48035393638755863
1101	50-51	0.42197516206603325
1101	52-53	0.4310316992868124
1101	54-55	0.39518285553867827
1101	56-57	0.4256198670287503
1101	58-59	0.4131679020061725
1101	60-61	0.3109934774537706
1101	62-63	0.35776507363195975
1101	64-65	0.36400171352468647
1101	66-67	0.4154477136972403
1101	68-69	0.39910863002281616
1101	70-71	0.3847094241566751
1101	72-73	0.38621295027004976
1101	74-75	0.40084725534989474
1101	76-77	0.4071053859536704
1101	78-79	0.3470073360906767
1101	80-81	0.33322234378383087
1101	82-83	0.3571275332337791
1101	84-85	0.30100143773518795
1101	86-87	0.27291334938662715
1101	88-89	0.3253787870963407
1101	90-91	0.33908723620815806
1101	92-93	0.3567896220078737
1101	94-95	0.398996678714429
1101	96-97	0.3600794894067114
1101	98-99	0.37866440163660897
1101	100	0.3905102585701776
1102	1	0.2439684537444542
1102	2	0.2717622804368318
1102	3	0.35207012008896044
1102	4	0.20656603835292486
1102	5	0.21350478477399548
1102	6	0.19587158748732492
1102	7	0.212560801926287
1102	8	0.1787875302780293
1102	9	0.2540562035657885
1102	10-11	0.2443208636367089
1102	12-13	0.2719724894514499
1102	14-15	0.3365136791797383
1102	16-17	0.3610954977638485
1102	18-19	0.3473242331680453
1102	20-21	0.3245867975834926
1102	22-23	0.2981823448009706
1102	24-25	0.27224881842487747
1102	26-27	0.2885033447645995
1102	28-29	0.2874614634798718
1102	30-31	0.29492044973320475
1102	32-33	0.3040987947559657
1102	34-35	0.3069637359232402
1102	36-37	0.36764160046048744
1102	38-39	0.27575700416146276
1102	40-41	0.34001075922644475
1102	42-43	0.29267975368650667
1102	44-45	0.3101072064543402
1102	46-47	0.29708567409740283
1102	48-49	0.292494057381532
1102	50-51	0.2583140702969544
1102	52-53	0.2379521215035183
1102	54-55	0.2611529690978571
1102	56-57	0.24808242455431184
1102	58-59	0.22997206349902655
1102	60-61	0.14490099838938875
1102	62-63	0.1630014079730273
1102	64-65	0.08885990987526782
1102	66-67	0.15795127470435943
1102	68-69	0.20815203804918525
1102	70-71	0.2248562918267183
1102	72-73	0.23564848981314412
1102	74-75	0.1716510935426001
1102	76-77	0.16941764074518773
1102	78-79	0.15472560797391566
1102	80-81	0.1736538498254987
1102	82-83	0.15992285462677813
1102	84-85	0.09444187023770212
1102	86-87	0.09545705718530684
1102	88-89	0.1493327118438046
1102	90-91	0.12463653643496997
1102	92-93	0.1150077012396622
1102	94-95	0.0709015988554178
1102	96-97	0.14637006869523717
1102	98-99	0.15825077951800992
1102	100	0.13017813410626644
1103	1	0.31200039873484897
1103	2	0.3149762876655018
1103	3	0.3655558889954946
1103	4	0.20658108020764132
1103	5	0.22945257118150408
1103	6	0.20933646419528884
1103	7	0.226548089247963
1103	8	0.18184776194504337
1103	9	0.2728960143500885
1103	10-11	0.2562209516493894
1103	12-13	0.2541760604194536
1103	14-15	0.349067269088728
1103	16-17	0.3386495640862819
1103	18-19	0.33837843673584445
1103	20-21	0.3130302224804353
1103	22-23	0.33467997901041
1103	24-25	0.3431905633288821
1103	26-27	0.33849515362290816
1103	28-29	0.34985953394930647
1103	30-31	0.3492636202062229
1103	32-33	0.3585186337869928
1103	34-35	0.3799908312691187
1103	36-37	0.3423896411400591
1103	38-39	0.33285770713376195
1103	40-41	0.372276620508984
1103	42-43	0.3878946016214755
1103	44-45	0.34725433448026877
1103	46-47	0.42388937439366714
1103	48-49	0.38239906456933426
1103	50-51	0.3726859285847226
1103	52-53	0.3425318908428139
1103	54-55	0.4061251235081045
1103	56-57	0.38753026302723725
1103	58-59	0.37564422541699827
1103	60-61	0.3418596119609063
1103	62-63	0.4280820988075149
1103	64-65	0.39643957838527655
1103	66-67	0.3447831081552408
1103	68-69	0.38813997287054747
1103	70-71	0.3532487962816617
1103	72-73	0.3726101302442828
1103	74-75	0.3462760326868093
1103	76-77	0.3421733950304855
1103	78-79	0.36941419701030753
1103	80-81	0.3519613427095081
1103	82-83	0.2794910596323348
1103	84-85	0.3363205794035764
1103	86-87	0.28075562512545815
1103	88-89	0.3021141772677751
1103	90-91	0.2808897781428499
1103	92-93	0.3226689444086688
1103	94-95	0.30334717662606536
1103	96-97	0.308049933521918
1103	98-99	0.3601044338930315
1103	100	0.31909871859391714
1104	1	0.24068356123877876
1104	2	0.25571414708432627
1104	3	0.3197763961579696
1104	4	0.20312062604802605
1104	5	0.21607316631854445
1104	6	0.19310370239369945
1104	7	0.2242320315264692
1104	8	0.19386174335768658
1104	9	0.28025061547448615
1104	10-11	0.2617751641588626
1104	12-13	0.21287599989149442
1104	14-15	0.3478027255658489
1104	16-17	0.34890187296426234
1104	18-19	0.29367008878552525
1104	20-21	0.2958724553622005
1104	22-23	0.28680545546357195
1104	24-25	0.30578901214095566
1104	26-27	0.31941903199261645
1104	28-29	0.2898352500604702
1104	30-31	0.2774240247438158
1104	32-33	0.3073565151994515
1104	34-35	0.3311854859053227
1104	36-37	0.31306357358504044
1104	38-39	0.3130318022589904
1104	40-41	0.34058596380791784
1104	42-43	0.3409098208581014
1104	44-45	0.3606235604676655
1104	46-47	0.336235441621497
1104	48-49	0.343255471535322
1104	50-51	0.3662792352469779
1104	52-53	0.3861251066222522
1104	54-55	0.3846675085277127
1104	56-57	0.36636619954740723
1104	58-59	0.3651067262576646
1104	60-61	0.32660711222901284
1104	62-63	0.31230847699999487
1104	64-65	0.32386274150721306
1104	66-67	0.3681923407589025
1104	68-69	0.364454274736012
1104	70-71	0.3261273641138729
1104	72-73	0.31743044582577795
1104	74-75	0.3603428751814306
1104	76-77	0.3422637325510891
1104	78-79	0.35491238935106395
1104	80-81	0.3200058534782144
1104	82-83	0.29276213217681857
1104	84-85	0.24389711636834477
1104	86-87	0.2780822161655081
1104	88-89	0.3533820834548962
1104	90-91	0.3066659628458197
1104	92-93	0.3019080371073315
1104	94-95	0.3011761678813194
1104	96-97	0.2880493518718872
1104	98-99	0.31773341024350543
1104	100	0.2545326567826933
1105	1	0.18665163226033243
1105	2	0.19458889687388847
1105	3	0.2818813201264305
1105	4	0.17408695859948864
1105	5	0.18779316485633046
1105	6	0.12359474600041409
1105	7	0.1768133723413996
1105	8	0.11439762248043195
1105	9	0.20530581454865882
1105	10-11	0.1727342616718417
1105	12-13	0.1655280964089343
1105	14-15	0.18843245894647254
1105	16-17	0.22003184873780413
1105	18-19	0.2188291238358815
1105	20-21	0.2377164926967552
1105	22-23	0.2069613049634924
1105	24-25	0.23186484421245268
1105	26-27	0.2020159116970106
1105	28-29	0.25889212310303833
1105	30-31	0.28573427504077387
1105	32-33	0.2541480573968471
1105	34-35	0.24261101890972014
1105	36-37	0.25276883561161867
1105	38-39	0.22720229568893302
1105	40-41	0.269121929336535
1105	42-43	0.24121466066560515
1105	44-45	0.17945734690105297
1105	46-47	0.17706240342006652
1105	48-49	0.22823821754506213
1105	50-51	0.19829263498577632
1105	52-53	0.17353622926854229
1105	54-55	0.2057155482654025
1105	56-57	0.13590933687208917
1105	58-59	0.1877986328018224
1105	60-61	0.207151138156199
1105	62-63	0.16674466581198288
1105	64-65	0.12388319388286106
1105	66-67	0.10166647674956408
1105	68-69	0.16576700703426894
1105	70-71	0.2077726531783739
1105	72-73	0.2481147947140272
1105	74-75	0.24540607575869444
1105	76-77	0.2580032565387853
1105	78-79	0.17732382796724977
1105	80-81	0.18898167016351408
1105	82-83	0.1266796576541651
1105	84-85	0.1437225101595203
1105	86-87	0.1326108226893652
1105	88-89	0.06799719416792271
1105	90-91	0.08822850642486912
1105	92-93	0.1346890820330131
1105	94-95	0.1630110589803202
1105	96-97	0.17885149559470648
1105	98-99	0.2679420345251309
1105	100	0.23453666056135347
1106	1	0.14691319117046575
1106	2	0.202982971137196
1106	3	0.2282983826675249
1106	4	0.1288170857443447
1106	5	0.1501649831144931
1106	6	0.13532996769482253
1106	7	0.13897063605867999
1106	8	0.10324136557432695
1106	9	0.10359185612325916
1106	10-11	0.12622956204003088
1106	12-13	0.12453168292539374
1106	14-15	0.16984699186161123
1106	16-17	0.1736892726846122
1106	18-19	0.18008481479920846
1106	20-21	0.13789734128805264
1106	22-23	0.1877004967336049
1106	24-25	0.16363117139191985
1106	26-27	0.1183932764929807
1106	28-29	0.16396163762743043
1106	30-31	0.19324672371626406
1106	32-33	0.204861232589856
1106	34-35	0.15627533047317854
1106	36-37	0.16138275732491536
1106	38-39	0.14425214607707204
1106	40-41	0.17399490884319846
1106	42-43	0.15777127077289776
1106	44-45	0.19052540023962905
1106	46-47	0.17269625152867718
1106	48-49	0.18818984760757473
1106	50-51	0.13064143123239091
1106	52-53	0.07433569602503809
1106	54-55	0.1605628747132144
1106	56-57	0.19451265481134783
1106	58-59	0.13772102837457822
1106	60-61	0.1411044712866527
1106	62-63	0.13045297547888168
1106	64-65	0.09330866788639014
1106	66-67	0.10810563837360831
1106	68-69	0.08631335156228914
1106	70-71	0.15320528559530544
1106	72-73	0.1848824344631339
1106	74-75	0.24344512765073745
1106	76-77	0.19181485462212322
1106	78-79	0.16584336444927317
1106	80-81	0.10342344508758572
1106	82-83	0.11963512461820613
1106	84-85	0.1336872054478775
1106	86-87	0.12765607937918588
1106	88-89	0.12472885649969356
1106	90-91	0.11277440430504271
1106	92-93	0.1545365644646779
1106	94-95	0.08937889109522246
1106	96-97	0.15865743708849323
1106	98-99	0.177982672691261
1106	100	0.14469077364812577
1107	1	0.1423835364966557
1107	2	0.1585051445172283
1107	3	0.25277490870146835
1107	4	0.12224252483251519
1107	5	0.15463395041983574
1107	6	0.0709546370447498
1107	7	0.06377160967945628
1107	8	0.08774371237225154
1107	9	0.06039839973460914
1107	10-11	0.058617137914616535
1107	12-13	0.05576715690420997
1107	14-15	0.15036239786348204
1107	16-17	0.10569309150289996
1107	18-19	0.10963932399086929
1107	20-21	0.13491311000190365
1107	22-23	0.11789371306433338
1107	24-25	0.14693516695898978
1107	26-27	0.10135984741154402
1107	28-29	0.15275548077593726
1107	30-31	0.19260812734076893
1107	32-33	0.12709276793741253
1107	34-35	0.1199860785029756
1107	36-37	0.1268949033883331
1107	38-39	0.13505334106995548
1107	40-41	0.12388770021502893
1107	42-43	0.17426964527851396
1107	44-45	0.25787136594573923
1107	46-47	0.21791565142952152
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2216	80-81	-0.5982093045779493
2216	82-83	-0.594937733324123
2216	84-85	-0.6704188993815166
2216	86-87	-0.6739165528462472
2216	88-89	-0.6974094509274877
2216	90-91	-0.6522506039153022
2216	92-93	-0.6784464444483049
2216	94-95	-0.6770554291829178
2216	96-97	-0.6723728369618236
2216	98-99	-0.7778097066177594
2216	100	-0.793688802156062
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	442.0
3	64.0
4	80.0
5	142.0
6	482.0
7	1223.0
8	2598.0
9	4471.0
10	5896.0
11	7691.0
12	11191.0
13	15664.0
14	19173.0
15	25456.0
16	32101.0
17	44601.0
18	45062.0
19	23875.0
20	16378.0
21	15356.0
22	15905.0
23	17421.0
24	20171.0
25	24610.0
26	31819.0
27	40776.0
28	52930.0
29	69746.0
30	91224.0
31	118698.0
32	154280.0
33	203325.0
34	278584.0
35	411620.0
36	693798.0
37	1303893.0
38	1844530.0
39	755022.0
40	2233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59761214980016	20.524274298145997	15.04591454243557	22.832199009618268
2	15.694027877412855	20.86104698282601	39.66993677968916	23.774988360071976
3	17.87068270344962	25.70541243767504	31.106260945866566	25.317643913008776
4	12.762515324017954	17.9939152188408	38.225851620242054	31.017717836899187
5	12.790959594160844	37.53537315120808	35.50862983047755	14.165037424153528
6	29.880473607341113	38.432982393336125	17.57561359833528	14.110930400987487
7	25.965184705860857	33.24860121723737	22.63945305380013	18.146761023101647
8	24.68458176930342	35.76541839469422	20.463657263041757	19.0863425729606
9	24.70871285121462	16.759637712023572	21.924329612773448	36.607319823988355
10-11	23.4964500757591	27.099720407445115	28.85511995178157	20.54870956501421
12-13	24.543293894242762	24.861230660187353	28.98636492349666	21.609110522073223
14-15	21.82504856282617	26.730975609489434	27.067483156270544	24.376492671413853
16-17	20.573129595155415	29.865040872117603	27.77313378100005	21.788695751726934
18-19	20.376215281113048	27.982058970116665	31.011321928780976	20.63040381998931
20-21	21.40139579175798	27.246646677696678	30.882076166441053	20.46988136410429
22-23	21.176836160574624	27.110544251952863	30.910338427100157	20.80228116037236
24-25	21.055118671038063	27.171738801420876	30.86348195736967	20.90966057017139
26-27	20.792402254670847	27.188052662298706	30.975008164739847	21.044536918290596
28-29	20.810988654330608	27.1193923153203	31.181965382127785	20.887653648221306
30-31	21.104200823080745	27.408559208850374	30.72092895762629	20.76631101044259
32-33	20.747787086075803	27.30378814253301	31.01700718044161	20.93141759094958
34-35	21.050534546416095	27.032379851030786	30.91566444582619	21.001421156726927
36-37	21.331415654215498	27.27279258780629	30.431152929989718	20.964638827988495
38-39	21.142701222375965	27.43475978484134	30.471754061011186	20.950784931771512
40-41	21.138640328332656	27.18011830009101	30.50688079448581	21.17436057709053
42-43	21.130315495543872	27.31278458472126	30.29881464064758	21.258085279087286
44-45	21.292977730213256	27.455118920939235	29.995340905026463	21.256562443821046
46-47	21.490134136015897	27.593907784280937	29.562621407065425	21.35333667263774
48-49	21.49958352407821	27.604247445268125	29.245082921113543	21.651086109540117
50-51	21.517568599043095	27.675406803965497	29.03501755790015	21.77200703909126
52-53	21.51691260846687	27.794094241792816	28.951761420600697	21.737231729139616
54-55	21.564706207591968	28.03749798321945	28.805631710334556	21.59216409885403
56-57	21.485651533745013	27.75272778843242	28.88756024765831	21.874060430164256
58-59	21.401059987058243	27.413096477002615	29.144279035900023	22.041564500039126
60-61	21.501910728741493	27.739850068668154	28.87273798440023	21.885501218190118
62-63	21.362364352472483	27.69890532353533	29.016735725293636	21.921994598698546
64-65	21.38500383676393	27.52551295729767	29.045427503591938	22.044055702346462
66-67	21.30382500295586	27.548847479223447	28.99935197502363	22.14797554279706
68-69	21.615139387845215	27.682193182664793	28.820094740658032	21.882572688831964
70-71	21.61378835963465	27.486934463886236	28.796674315204406	22.102602861274708
72-73	21.452180395534203	27.60113929944267	28.88050834896387	22.066171956059254
74-75	21.5566390853867	27.599757033585625	28.666233712886356	22.177370168141316
76-77	21.59346046118324	27.62771472719148	28.5151450262404	22.263679785384873
78-79	21.65028174014308	27.6010768241497	28.66927938341884	22.079362052288385
80-81	21.42823785450695	27.54066016828689	28.712292771017157	22.318809206189
82-83	21.359292600443702	27.60204151758409	28.800332745437245	22.238333136534962
84-85	21.350111042509468	27.458198770074766	28.67991627649374	22.511773910922024
86-87	21.445073921170742	27.59257483331501	28.588288067653618	22.374063177860634
88-89	21.26664266036099	27.47685683581116	28.60195913008458	22.65454137374327
90-91	21.156998628422006	27.398252505142732	28.63621820265102	22.80853066378424
92-93	21.22979845802722	27.474425079216946	28.544567464725763	22.75120899803007
94-95	21.028477010325084	27.32062109025741	28.709278407838294	22.941623491579207
96-97	21.02442006465931	27.38936671580908	28.615008775504368	22.971204444027247
98-99	20.967174863462233	27.38688259680956	28.54230909742081	23.103633442307395
100	21.03907771372665	27.282053140292795	28.516082095123735	23.162787050856817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	966.0
1	734.0
2	905.0
3	1292.5
4	871.5
5	612.0
6	1455.0
7	3728.5
8	6252.0
9	7443.0
10	6080.5
11	4006.0
12	3770.5
13	4271.0
14	4821.5
15	5493.5
16	6233.5
17	7024.0
18	8094.5
19	9324.0
20	10846.5
21	13199.5
22	16431.5
23	21286.5
24	28234.5
25	37255.0
26	49544.0
27	63369.5
28	80275.5
29	100457.5
30	117574.0
31	133627.5
32	149965.5
33	164538.5
34	177033.0
35	187170.0
36	197561.0
37	208064.0
38	216806.5
39	221206.5
40	224751.0
41	227351.5
42	225009.5
43	222969.0
44	220474.5
45	216680.5
46	212669.5
47	206782.5
48	201848.5
49	198300.5
50	192965.0
51	183848.0
52	170647.0
53	159954.5
54	153180.5
55	145386.5
56	133512.0
57	118941.5
58	108196.5
59	99962.0
60	88814.0
61	75645.0
62	64409.0
63	54377.5
64	44702.0
65	36955.0
66	30152.5
67	23973.5
68	19172.5
69	15197.0
70	11747.0
71	8985.5
72	7008.0
73	5521.5
74	4365.0
75	3262.0
76	2245.0
77	1673.0
78	1308.5
79	1014.0
80	645.5
81	278.5
82	138.5
83	59.5
84	29.5
85	16.0
86	19.5
87	16.0
88	7.5
89	6.0
90	2.5
91	1.0
92	1.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12221729188035169
2	0.0
3	0.0
4	0.0
5	0.012229538599656918
6	1.874258789219451E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.874258789219451E-4
82-83	6.091341064963215E-4
84-85	4.373270508178718E-4
86-87	5.466588135223399E-4
88-89	7.262752808225372E-4
90-91	8.043693970400143E-4
92-93	2.8894823000466535E-4
94-95	2.4209176027417909E-4
96-97	7.809411621747712E-4
98-99	0.0014056940919145882
100	1.0933176270446796E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6402531.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.02920995145389
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.95463821730192	42.17574589778328
2	15.440502406999537	17.611193070494117
3	4.867129266655868	8.327056104269502
4	2.0310596033656507	4.633188981770255
5	1.0478118164144135	2.9877940033955923
6	0.629627101422661	2.154428169489498
7	0.3941888482401697	1.5736195010767304
8	0.28968869582171364	1.3216573963663498
9	0.2023552374727588	1.0386143390349247
>10	1.0437356074683224	10.721260681963578
>50	0.0668605215865809	2.606880334830038
>100	0.030595247038603602	3.0563673023440705
>500	0.0011502028176863526	0.43982715180282295
>1k	5.476894956080739E-4	0.4586288320666205
>5k	2.7384474628819202E-5	0.09754471596545387
>10k+	8.21534238864576E-5	0.7961935173472254
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20161	0.31489109541211124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20004	0.3124389401628825	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10817	0.16894881102488998	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5618823243495423E-5	0.0
3	0.0	0.0	0.0	3.1237646486990846E-5	0.0
4	0.0	0.0	0.0	9.371293946097254E-5	0.0
5	0.0	0.0	0.0	1.0933176270446797E-4	0.0
6	0.0	0.0	0.0	1.0933176270446797E-4	0.0
7	0.0	0.0	0.0	1.2495058594796338E-4	0.0
8	0.0	0.0	0.0	1.2495058594796338E-4	0.0
9	0.0	0.0	0.0	1.874258789219451E-4	0.0
10-11	0.0	0.0	0.0	2.0304470216544053E-4	0.0
12-13	0.0	1.5618823243495423E-5	0.0	2.2647293703068365E-4	0.0
14-15	0.0	1.5618823243495423E-5	0.0	2.5771058351767447E-4	0.0
16-17	0.0	1.5618823243495423E-5	0.0	3.1237646486990847E-4	0.0
18-19	0.0	1.5618823243495423E-5	0.0	3.5142352297864706E-4	0.0
20-21	0.0	1.5618823243495423E-5	0.0	3.826611694656379E-4	0.0
22-23	0.0	5.4665881352233985E-5	0.0	4.998023437918536E-4	0.0
24-25	0.0	7.028470459572941E-5	0.0	8.902729248792392E-4	0.0
26-27	0.0	7.809411621747712E-5	0.0	0.0015931199708365332	0.0
28-29	0.0	7.809411621747712E-5	0.0	0.0038968963992521083	0.0
30-31	0.0	7.809411621747712E-5	0.0	0.009956999817728332	0.0
32-33	0.0	7.809411621747712E-5	0.0	0.025060401894188407	0.0
34-35	0.0	7.809411621747712E-5	0.0	0.05147183199893916	0.0
36-37	0.0	7.809411621747712E-5	0.0	0.09304913947312399	0.0
38-39	0.0	7.809411621747712E-5	0.0	0.14598133144533	0.0
40-41	0.0	7.809411621747712E-5	0.0	0.19948361046592356	0.0
42-43	0.0	7.809411621747712E-5	0.0	0.2554302353241241	0.0
44-45	0.0	8.590352783922482E-5	0.0	0.32903393985909635	0.0
46-47	0.0	1.0152235108272026E-4	0.0	0.42112252170274533	0.0
48-49	0.0	1.0933176270446797E-4	0.0	0.5197866281319059	0.0
50-51	0.0	1.7180705567844967E-4	0.0	0.6147725016872234	0.0
52-53	0.0	2.0304470216544053E-4	0.0	0.7103987469955241	0.0
54-55	0.0	2.2647293703068365E-4	0.0	0.8236820719805964	0.0
56-57	0.0	2.3428234865243135E-4	0.0	0.9635408247144762	0.0
58-59	0.0	2.3428234865243135E-4	0.0	1.116121108980183	0.0
60-61	0.0	2.499011718959268E-4	0.0	1.2737775108000258	0.0
62-63	0.0	2.8113881838291764E-4	0.0	1.4325584678934002	0.0
64-65	0.0	3.201858764916562E-4	0.0	1.609347928186525	0.0
66-67	0.0	3.4361411135689935E-4	0.0	1.813829562090367	0.0
68-69	0.0	3.5142352297864706E-4	0.0	2.0427234167237924	0.0
70-71	0.0	3.982799927091333E-4	0.0	2.2921560239224146	0.0
72-73	0.0	4.2170822757437647E-4	0.0	2.546711605144903	0.0
74-75	0.0	4.529458740613673E-4	0.0	2.811177329715389	0.0
76-77	0.0	4.685646973048627E-4	0.0	3.09871986562814	0.0
78-79	0.0	4.841835205483581E-4	0.0	3.4283629395937325	0.0
80-81	0.0	4.841835205483581E-4	0.0	3.7843081119013715	0.0
82-83	0.0	4.841835205483581E-4	0.0	4.1516472157651405	0.0
84-85	0.0	4.841835205483581E-4	0.0	4.520017161962979	0.0
86-87	0.0	4.998023437918535E-4	0.0	4.915017201790979	0.0
88	0.0	4.998023437918535E-4	0.0	5.233539673607203	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12640	0.0	60.541645	1
GTATCAA	22960	0.0	39.827374	1
GTGGTAT	4845	0.0	36.232296	1
ATCAACG	27810	0.0	32.503963	3
TCAACGC	27950	0.0	32.341156	4
TGGTATC	5215	0.0	31.818409	2
TATCAAC	29000	0.0	31.267437	2
CAACGCA	29305	0.0	30.82973	5
AACGCAG	30445	0.0	29.747873	6
ACGCAGA	34435	0.0	26.123337	7
CGCAGAG	34600	0.0	25.903679	8
GCAGAGT	37645	0.0	23.583675	9
GTACATG	17210	0.0	22.943615	1
GAGTACT	28170	0.0	22.089605	12-13
CAGAGTA	36710	0.0	21.995083	10-11
TACATGG	17495	0.0	21.333622	2
ACATGGG	17640	0.0	20.49207	3
GTACTTT	30545	0.0	20.272034	14-15
AGAGTAC	35490	0.0	19.970188	10-11
AGTACTT	29270	0.0	18.81879	12-13
>>END_MODULE