FastQCFastQC Report
Wed 25 May 2016
SRR2049436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10037069
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100700.036.146512
TATAACG11000.035.484632
GGTATCA90350.031.1783811
GTACCTG125150.030.0993121
GTATCAA133300.029.8818971
TCAACGC135300.028.3968734
ATCAACG138150.027.7094843
ACCTGGG135000.026.823283
TAACGCA14750.026.7814144
CAACGCA152050.025.3304985
AACGCAG159650.024.2273756
ATAACGC18250.023.9648673
GTACATG295100.023.2509651
TATCAAC165900.023.102882
TACATGG310900.021.4189152
GTATAAC29150.020.9729581
ACGCAGA183700.020.7218027
ACATGGG312300.020.44953
TATCACG17400.020.000342
CGCAGAG198050.018.9587888
Produced by FastQC (version 0.11.5)