##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049436_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10037069
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.867013069253584	31.0	30.0	34.0	26.0	34.0
2	30.00395563684976	31.0	30.0	34.0	26.0	34.0
3	30.097638264716522	31.0	30.0	34.0	26.0	34.0
4	33.739847160560515	35.0	35.0	37.0	28.0	37.0
5	33.7435360860825	35.0	35.0	37.0	28.0	37.0
6	33.787147522847555	37.0	35.0	37.0	29.0	37.0
7	33.66814017119938	37.0	35.0	37.0	29.0	37.0
8	33.61671220951057	37.0	35.0	37.0	28.0	37.0
9	34.961552222068015	38.0	35.0	39.0	28.0	39.0
10-11	34.871613515858066	38.0	35.0	39.0	27.0	39.0
12-13	34.7702863754349	38.0	35.0	39.0	27.0	39.0
14-15	35.65377287931367	39.0	35.5	41.0	27.0	41.0
16-17	35.694092020289986	39.0	36.0	41.0	27.0	41.0
18-19	35.75949462935843	39.0	36.0	41.0	27.0	41.0
20-21	35.89298364891185	39.0	36.0	41.0	27.0	41.0
22-23	35.82677393171254	39.0	36.0	41.0	27.0	41.0
24-25	35.73735694155336	39.0	35.0	41.0	27.0	41.0
26-27	35.581928748322845	39.0	35.0	41.0	27.0	41.0
28-29	35.48991812251166	39.0	35.0	41.0	27.0	41.0
30-31	35.46182765108021	39.0	35.0	41.0	26.5	41.0
32-33	35.35858834884965	39.0	35.0	40.0	26.5	41.0
34-35	35.29242730123704	39.0	35.0	40.0	25.5	41.0
36-37	35.183275466174436	39.0	34.5	40.0	25.5	41.0
38-39	35.04828087761477	39.0	34.0	40.0	25.0	41.0
40-41	35.01644075576246	39.0	34.0	40.0	25.0	41.0
42-43	34.877857021805866	38.5	34.0	40.0	24.5	41.0
44-45	34.76775246837498	38.0	34.0	40.0	24.5	41.0
46-47	34.53731941067656	38.0	33.0	40.0	24.0	41.0
48-49	34.46336236206008	38.0	33.0	40.0	23.5	41.0
50-51	34.380398002644	38.0	33.0	40.0	24.0	41.0
52-53	34.20987875045992	38.0	33.0	40.0	23.0	41.0
54-55	34.144728804793516	38.0	33.0	40.0	23.0	41.0
56-57	33.9151753863603	38.0	33.0	40.0	23.0	41.0
58-59	33.73725621493685	37.0	33.0	40.0	23.0	41.0
60-61	33.4527410342601	37.0	33.0	40.0	22.0	41.0
62-63	33.163518303998906	36.0	32.0	40.0	21.5	41.0
64-65	32.95533347434396	36.0	32.0	39.0	21.0	41.0
66-67	32.653764958674685	35.5	32.0	39.0	20.5	41.0
68-69	32.36577804735626	35.0	32.0	39.0	20.0	40.0
70-71	32.07788583499824	35.0	31.5	38.0	18.5	40.0
72-73	31.737971961735045	35.0	31.0	37.5	18.0	40.0
74-75	31.380321884805216	35.0	31.0	37.0	16.0	39.0
76-77	31.107753618113016	35.0	31.0	36.5	13.5	39.0
78-79	30.802060193070307	35.0	31.0	36.0	10.5	39.0
80-81	30.461152254707024	34.5	30.5	35.5	7.0	37.5
82-83	30.163646279606127	34.0	30.0	35.0	4.5	37.0
84-85	29.900075759168338	34.0	30.0	35.0	2.0	37.0
86-87	29.6747640172644	34.0	30.0	35.0	2.0	36.0
88-89	29.414615063421405	34.0	30.0	35.0	2.0	36.0
90-91	29.091185833234782	34.0	30.0	35.0	2.0	36.0
92-93	28.897885528135753	34.0	29.0	35.0	2.0	35.0
94-95	28.643345133923063	34.0	29.0	35.0	2.0	35.0
96-97	28.382336915288718	34.0	29.0	35.0	2.0	35.0
98-99	28.098127650611946	34.0	29.0	35.0	2.0	35.0
100	27.915893075956735	34.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5132288549808024
1101	2	0.4867533558249022
1101	3	0.45728390804171326
1101	4	0.4321191607700854
1101	5	0.41305599695144934
1101	6	0.43883542529945885
1101	7	0.48378557421827395
1101	8	0.4750993788040674
1101	9	0.5920313530436658
1101	10-11	0.55835888701084
1101	12-13	0.5658844033220163
1101	14-15	0.8201434194170929
1101	16-17	0.7621346776391675
1101	18-19	0.670229368667556
1101	20-21	0.539533455182962
1101	22-23	0.48991441928488655
1101	24-25	0.5284260999392032
1101	26-27	0.6475361911892676
1101	28-29	0.7592521989478058
1101	30-31	0.705579438312526
1101	32-33	0.7122332977899006
1101	34-35	0.686656698228461
1101	36-37	0.694276192514721
1101	38-39	0.7421267533709397
1101	40-41	0.7118856386415402
1101	42-43	0.7503481284427593
1101	44-45	0.6387522715447247
1101	46-47	0.7394434178368101
1101	48-49	0.6652005222522561
1101	50-51	0.5823497265825921
1101	52-53	0.6141175286659575
1101	54-55	0.43179028213221926
1101	56-57	0.5120779030169942
1101	58-59	0.4452764954537187
1101	60-61	0.47924465510747893
1101	62-63	0.5651496324310585
1101	64-65	0.5488452440197449
1101	66-67	0.5624635936536464
1101	68-69	0.47575653894669756
1101	70-71	0.46302993871977094
1101	72-73	0.39590944938908024
1101	74-75	0.422471151224606
1101	76-77	0.3905038059010373
1101	78-79	0.30074487459881993
1101	80-81	0.4161229062887486
1101	82-83	0.5331300052801744
1101	84-85	0.48397856731835276
1101	86-87	0.44679913014696027
1101	88-89	0.45865608669468116
1101	90-91	0.5240289605215267
1101	92-93	0.5113811149272465
1101	94-95	0.4049130017381586
1101	96-97	0.41706726725652743
1101	98-99	0.3658286581767918
1101	100	0.4469449042970304
1102	1	0.4898728980164613
1102	2	0.5279754285355551
1102	3	0.5160740502480472
1102	4	0.45820798134118235
1102	5	0.513299507140168
1102	6	0.43950001864543964
1102	7	0.4119118844836507
1102	8	0.4959528853872044
1102	9	0.5855360076298268
1102	10-11	0.6260476944024518
1102	12-13	0.6121354836863162
1102	14-15	0.7704217505128739
1102	16-17	0.7835054758556836
1102	18-19	0.6703042643470951
1102	20-21	0.48500839038955235
1102	22-23	0.5952562275455762
1102	24-25	0.565247409503506
1102	26-27	0.6487515725837696
1102	28-29	0.7208061247475683
1102	30-31	0.6712903481881156
1102	32-33	0.7289986768057162
1102	34-35	0.6892057476785851
1102	36-37	0.7250529960772383
1102	38-39	0.7609084543952918
1102	40-41	0.5874861698268177
1102	42-43	0.6448412495068467
1102	44-45	0.6091981333710024
1102	46-47	0.6716868636077038
1102	48-49	0.6939891510380534
1102	50-51	0.5885475236750963
1102	52-53	0.6531825218690841
1102	54-55	0.47910646882918684
1102	56-57	0.4511488272693711
1102	58-59	0.4543730235150818
1102	60-61	0.43415363619276803
1102	62-63	0.4925547988088681
1102	64-65	0.3507651640710776
1102	66-67	0.441976476251142
1102	68-69	0.46171072171213723
1102	70-71	0.4053823521916087
1102	72-73	0.4130951020713809
1102	74-75	0.4372715162892966
1102	76-77	0.4036644684508808
1102	78-79	0.45274829170264397
1102	80-81	0.49871202026823624
1102	82-83	0.5401080220146923
1102	84-85	0.505178277028417
1102	86-87	0.4914890883527683
1102	88-89	0.46775620696025655
1102	90-91	0.487094222663373
1102	92-93	0.43892442823187494
1102	94-95	0.43277180446801466
1102	96-97	0.3098282772415182
1102	98-99	0.26501892413135764
1102	100	0.2074020597288211
1103	1	0.25478783184645337
1103	2	0.26318061522327696
1103	3	0.24332546384791343
1103	4	0.21754469345322036
1103	5	0.22080331283055443
1103	6	0.1868536040464761
1103	7	0.14669084115674025
1103	8	0.22388166261318077
1103	9	0.2930196444858453
1103	10-11	0.2808743346975646
1103	12-13	0.2813704440992524
1103	14-15	0.5230420369842435
1103	16-17	0.4332472016335487
1103	18-19	0.364322484633135
1103	20-21	0.2101533976325527
1103	22-23	0.2700809909444146
1103	24-25	0.3050996853709407
1103	26-27	0.4288497243918954
1103	28-29	0.3736731343912396
1103	30-31	0.3581329469644956
1103	32-33	0.38945866228106496
1103	34-35	0.41931003235841047
1103	36-37	0.3748635840181578
1103	38-39	0.37898808283674157
1103	40-41	0.32887440432956794
1103	42-43	0.3898792981182879
1103	44-45	0.2996319231145108
1103	46-47	0.36929728681631957
1103	48-49	0.3169879300716332
1103	50-51	0.3253787470883367
1103	52-53	0.29043950024009035
1103	54-55	0.17394656560192345
1103	56-57	0.21489427791471627
1103	58-59	0.1263666307113951
1103	60-61	0.26640913858458504
1103	62-63	0.1818217687782635
1103	64-65	0.18764245675629354
1103	66-67	0.20117038317741276
1103	68-69	0.2556818692036984
1103	70-71	0.22168081394764272
1103	72-73	0.16471442405082826
1103	74-75	0.1260754252820604
1103	76-77	0.1219009752863549
1103	78-79	0.09036217411232172
1103	80-81	0.19050645439237357
1103	82-83	0.12668937746352427
1103	84-85	0.04980831644534689
1103	86-87	0.08145295409399367
1103	88-89	0.03703890074968896
1103	90-91	0.10486736212459391
1103	92-93	0.0834858697185652
1103	94-95	0.08081214500395362
1103	96-97	0.00683466845700309
1103	98-99	0.0032067441034122623
1103	100	-0.010574732956236232
1104	1	0.2832155369107099
1104	2	0.25448640900924246
1104	3	0.2998877956959305
1104	4	0.31171087658766083
1104	5	0.2891692426553121
1104	6	0.2716665828014726
1104	7	0.25492243024449834
1104	8	0.248995886891052
1104	9	0.40714016137822995
1104	10-11	0.4350076689328475
1104	12-13	0.4723562391810816
1104	14-15	0.6262567359221052
1104	16-17	0.5480101916928248
1104	18-19	0.48493274256622243
1104	20-21	0.3463613789353275
1104	22-23	0.3610823998719539
1104	24-25	0.4249650459782899
1104	26-27	0.5077188553771492
1104	28-29	0.47865964357757207
1104	30-31	0.4483515905037123
1104	32-33	0.5448400342590602
1104	34-35	0.5169951566338966
1104	36-37	0.48100613212506715
1104	38-39	0.5448372498659069
1104	40-41	0.5018584062810874
1104	42-43	0.43959441100943053
1104	44-45	0.4332768361921424
1104	46-47	0.561557460239996
1104	48-49	0.510530240441291
1104	50-51	0.49335254297805875
1104	52-53	0.5307470386996016
1104	54-55	0.3346214247287307
1104	56-57	0.3304977926268151
1104	58-59	0.2744582073660524
1104	60-61	0.37439384898042505
1104	62-63	0.3965557676120852
1104	64-65	0.364600536778795
1104	66-67	0.41476729092728704
1104	68-69	0.32119828599131495
1104	70-71	0.2979543743227637
1104	72-73	0.30242915987196284
1104	74-75	0.40282224670480815
1104	76-77	0.4249965594027145
1104	78-79	0.3442956345560759
1104	80-81	0.3753146104800287
1104	82-83	0.3604861792527707
1104	84-85	0.30807945784783897
1104	86-87	0.31133543194918545
1104	88-89	0.2980169413689744
1104	90-91	0.3654828743721694
1104	92-93	0.36011129153514077
1104	94-95	0.30142207621053885
1104	96-97	0.3474312839477136
1104	98-99	0.32443907090281243
1104	100	0.3645989210329361
1105	1	0.10738325872175736
1105	2	0.10365510662364841
1105	3	0.06499953338831332
1105	4	0.012081754821629431
1105	5	0.031271920150295784
1105	6	0.10183338863411961
1105	7	0.07142960321996128
1105	8	0.06583340122259784
1105	9	0.23187734196677923
1105	10-11	0.2125664077352738
1105	12-13	0.22630327257471095
1105	14-15	0.32128820512448186
1105	16-17	0.28963223131518134
1105	18-19	0.2547834601556005
1105	20-21	0.12961665255971866
1105	22-23	0.25839154459033864
1105	24-25	0.20659099246195467
1105	26-27	0.3114171455507915
1105	28-29	0.3857990404617908
1105	30-31	0.3176649049119291
1105	32-33	0.34034028577921305
1105	34-35	0.3090723492458096
1105	36-37	0.25850958300348736
1105	38-39	0.35116801127341546
1105	40-41	0.2854211537201934
1105	42-43	0.2807685249708598
1105	44-45	0.2937138146161331
1105	46-47	0.4169167318857774
1105	48-49	0.3007392256021504
1105	50-51	0.18427624026233502
1105	52-53	0.14635550590038804
1105	54-55	0.06256653606332918
1105	56-57	0.08542312360654591
1105	58-59	-0.0071815234800212124
1105	60-61	0.055011881790029804
1105	62-63	0.07970514833262143
1105	64-65	0.11166909125105917
1105	66-67	0.12432199263970034
1105	68-69	0.14499833056819966
1105	70-71	0.034395012100546296
1105	72-73	0.0258251853792828
1105	74-75	0.1299317238164761
1105	76-77	0.05705570330033183
1105	78-79	0.03775565452447438
1105	80-81	0.07160356646527433
1105	82-83	0.07469520764080784
1105	84-85	0.00438401756718676
1105	86-87	0.06558588090992501
1105	88-89	-0.003955686161230432
1105	90-91	0.02262025581205762
1105	92-93	-0.03518295148038675
1105	94-95	-0.14672344142304894
1105	96-97	-0.20943462808552837
1105	98-99	-0.20353105565249763
1105	100	-0.22700516654350267
1106	1	0.15395875925781155
1106	2	0.14388354704249195
1106	3	0.11784787167804467
1106	4	0.1803722842630222
1106	5	0.21717268619388364
1106	6	0.18407219739581393
1106	7	0.11151341483338939
1106	8	0.12352777191046727
1106	9	0.14530869826572967
1106	10-11	0.17930175561726713
1106	12-13	0.12745055898145807
1106	14-15	0.2040201357680047
1106	16-17	0.25026859429684123
1106	18-19	0.19061498871922566
1106	20-21	0.04569097800688127
1106	22-23	0.08870468342131232
1106	24-25	0.0918983208803823
1106	26-27	0.19096506578749484
1106	28-29	0.196407001699761
1106	30-31	0.20693568604506396
1106	32-33	0.1686047852603494
1106	34-35	0.19864423884506266
1106	36-37	0.193678404214765
1106	38-39	0.24323540614692263
1106	40-41	0.11924002277561385
1106	42-43	0.08120858673074594
1106	44-45	0.036804886907312095
1106	46-47	0.20189751415186663
1106	48-49	0.1568319115444936
1106	50-51	0.10533379661489306
1106	52-53	0.0996789640584268
1106	54-55	0.013285573943718987
1106	56-57	-0.0609464542106295
1106	58-59	-0.11287220745011695
1106	60-61	-0.19294301261857782
1106	62-63	-0.030237439782389686
1106	64-65	-0.05655709153954547
1106	66-67	-0.04607148585344589
1106	68-69	-0.043860876541323535
1106	70-71	-0.1405346298284158
1106	72-73	-0.20566066142102812
1106	74-75	-0.20140088416926716
1106	76-77	-0.24848543953703128
1106	78-79	-0.25069711930660077
1106	80-81	-0.17344379718431924
1106	82-83	-0.11547065703736692
1106	84-85	-0.1668271161958046
1106	86-87	-0.15822597276162398
1106	88-89	-0.14834003073530155
1106	90-91	-0.10850612579343633
1106	92-93	-0.1996580295347954
1106	94-95	-0.16375217993524416
1106	96-97	-0.2259911194197315
1106	98-99	-0.15454760105884802
1106	100	-0.12449604756564625
1107	1	-0.0012544611863383182
1107	2	0.027789883293404927
1107	3	-0.05315539816236381
1107	4	0.02945029994526749
1107	5	-0.0858470027571272
1107	6	-0.11013572011190575
1107	7	-0.010508080556633104
1107	8	-0.0011802439994568203
1107	9	0.12561134842399468
1107	10-11	0.06412304300147298
1107	12-13	0.06390560811060197
1107	14-15	0.293121039040976
1107	16-17	0.25217756809217917
1107	18-19	0.15894325802607057
1107	20-21	0.047829068312609024
1107	22-23	0.08901198924916542
1107	24-25	0.09789832898959361
1107	26-27	0.17488979703497165
1107	28-29	0.09918318473953036
1107	30-31	0.04474504089775877
1107	32-33	-0.0283403264492037
1107	34-35	-0.0067344407724618804
1107	36-37	0.005986563916223986
1107	38-39	0.13476188476089845
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2207	94-95	1.1055942188512446
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2207	100	1.1787743196966431
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2208	3	0.6251461601667749
2208	4	0.5484976448870427
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2208	6	0.4803257247738131
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2208	12-13	0.7828998692101266
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2208	22-23	0.8122687084684941
2208	24-25	0.941570991156091
2208	26-27	1.0700642033409196
2208	28-29	1.0460918421361995
2208	30-31	1.0017417752483624
2208	32-33	1.0443436009884195
2208	34-35	1.0503094247598739
2208	36-37	0.5524379299952642
2208	38-39	0.9748333004164635
2208	40-41	1.005668711615968
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2208	46-47	1.2155169288854921
2208	48-49	1.2076432375720643
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2208	54-55	1.0543670944637356
2208	56-57	1.0546609407057659
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2208	60-61	1.133798201248176
2208	62-63	1.1477783160567583
2208	64-65	1.074664431547788
2208	66-67	1.049291402012983
2208	68-69	1.0873535125164864
2208	70-71	1.0831226899902617
2208	72-73	1.0915648275285434
2208	74-75	1.1142289024325152
2208	76-77	1.0585723698664786
2208	78-79	1.0511769479366713
2208	80-81	1.013577448613745
2208	82-83	1.0373881660188857
2208	84-85	1.045576853831676
2208	86-87	0.9882438861282132
2208	88-89	0.9932442578704404
2208	90-91	1.1109303401733541
2208	92-93	1.0307605344370145
2208	94-95	0.5106834154918971
2208	96-97	0.8670051334870905
2208	98-99	1.0474257361463728
2208	100	1.0960060860968284
2209	1	0.5364460684754171
2209	2	0.4987590212101374
2209	3	0.44929801919634116
2209	4	0.39121232247252635
2209	5	0.4126334743015434
2209	6	0.426450979654696
2209	7	0.5010212715782174
2209	8	0.4713484534226495
2209	9	0.5567177601617956
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2209	12-13	0.6210122082581151
2209	14-15	0.8652836558690566
2209	16-17	0.7878118305486623
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2209	28-29	0.9123177184175901
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2209	86-87	0.7585564400685847
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2210	3	0.43716140482145605
2210	4	0.4017151652949238
2210	5	0.3928450934902017
2210	6	0.41119909771317253
2210	7	0.3856241913658991
2210	8	0.3889556728620036
2210	9	0.5464808122283458
2210	10-11	0.5477104087305307
2210	12-13	0.5417868065141889
2210	14-15	0.8186899390265481
2210	16-17	0.7893089509431022
2210	18-19	0.7370913052398151
2210	20-21	0.5754526352994063
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2210	24-25	0.6220306048115631
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2210	28-29	0.8797058378017795
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2210	44-45	0.9122951237454089
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2210	48-49	0.6211086344427983
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2211	2	0.05903173540524875
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2211	56-57	0.506875402406358
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2214	96-97	-0.4748334783033208
2214	98-99	-0.42304465135844893
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2215	3	-0.16079559378878017
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2215	14-15	-3.2696446953423504
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2215	20-21	-0.09139890699424313
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2215	24-25	0.018561884214555846
2215	26-27	0.19323618034548673
2215	28-29	0.18764983433099758
2215	30-31	0.14395030180975965
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2215	100	0.14042985437213673
2216	1	-0.46264351181536867
2216	2	-0.3869302536533148
2216	3	-0.40843725928299435
2216	4	-0.47646882113994593
2216	5	-0.4968009295198996
2216	6	-0.3626964544399698
2216	7	-0.4054755775554355
2216	8	-0.3703013882984578
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2216	10-11	-0.4548609433712585
2216	12-13	-0.4339781560652938
2216	14-15	-0.1999860460463836
2216	16-17	-0.457747542696481
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2216	30-31	-0.3760969975243782
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2216	36-37	-0.6999419699304781
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2216	54-55	-0.5965248328918165
2216	56-57	-0.5330039332788417
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2216	62-63	-0.4498236315353381
2216	64-65	-0.6043112441260945
2216	66-67	-0.6056871568193074
2216	68-69	-0.6125760014284012
2216	70-71	-0.6854996992215163
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2216	74-75	-0.6602645039355224
2216	76-77	-0.651085314805993
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2216	80-81	-0.6091358198219758
2216	82-83	-0.5215790274348038
2216	84-85	-0.5963216303199097
2216	86-87	-0.6011916015430323
2216	88-89	-0.5601092721734098
2216	90-91	-0.5363627829230424
2216	92-93	-0.614317620792928
2216	94-95	-0.5288929779185167
2216	96-97	-0.6087020666435272
2216	98-99	-0.48282585659290334
2216	100	-0.448617493811593
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	260261.0
3	42699.0
4	27759.0
5	22481.0
6	21400.0
7	22295.0
8	24695.0
9	26902.0
10	29346.0
11	31394.0
12	33716.0
13	35060.0
14	36484.0
15	37179.0
16	38125.0
17	38343.0
18	38328.0
19	38886.0
20	40452.0
21	43470.0
22	50912.0
23	62660.0
24	78254.0
25	97868.0
26	120124.0
27	142054.0
28	172849.0
29	213679.0
30	259422.0
31	314227.0
32	394664.0
33	508387.0
34	674681.0
35	930011.0
36	1359396.0
37	1838085.0
38	1573435.0
39	356723.0
40	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.987252171279195	18.74013429037825	13.451265913585415	23.82134762475714
2	16.677270012334873	19.738357456609933	38.26684589912945	25.31752663192574
3	18.812481229564547	24.93881212203452	29.12413281835407	27.124573830046867
4	13.520966181145393	16.904468116824454	36.2723145908033	33.30225111122686
5	14.337688677498928	36.172312002082855	33.399847857506174	16.09015146291205
6	31.757441311564477	35.72447867212793	18.006157432695026	14.511922583612566
7	28.325899074034094	31.134430640709134	21.697440288676155	18.842229996580617
8	26.169968834602813	34.55357336064058	20.005137558835866	19.27132024592074
9	25.529056408252863	17.382680121690726	20.598196736399814	36.4900667336566
10-11	24.914329523828897	26.20041367512982	27.54997294878936	21.335283852251933
12-13	25.187345337088	23.8293328074167	27.54546026496108	23.43786159053422
14-15	22.79611878156753	25.666009806687352	24.828519564495302	26.709351847249817
16-17	22.98311941325805	28.551612175352144	25.13679787237543	23.328470539014376
18-19	23.04206290522854	27.8851843261404	26.56968219146029	22.503070577170764
20-21	23.930665288101576	27.26744254157817	26.062701946035062	22.739190224285196
22-23	23.694488941681826	27.119382617738992	26.023211854961346	23.16291658561783
24-25	23.37854863768345	27.14900312671641	26.176661222006636	23.295787013593504
26-27	23.33913231085291	27.084365526905625	26.187420780000114	23.38908138224135
28-29	23.23356421651342	27.152335542467466	26.310338897293956	23.303761343725153
30-31	23.414691183385248	27.454669383802795	25.980350458703704	23.15028897410826
32-33	23.213273417976133	27.249821380320626	26.176353132888703	23.36055206881454
34-35	23.390195624119663	26.896182908990557	26.235910628801502	23.47771083808828
36-37	23.61825986213456	27.100313980675416	26.130783506436885	23.150642650753138
38-39	23.415949384554402	27.183644492334146	26.120023855346158	23.280382267765294
40-41	23.37220266808318	26.950884283098205	26.272453030187016	23.4044600186316
42-43	23.224742802980373	27.08170099716006	26.301180905690074	23.392375294169497
44-45	23.237411313109472	27.192518040538534	26.152418492227625	23.41765215412437
46-47	23.27219415709491	27.00852386326264	26.30959908115505	23.409682898487404
48-49	23.183830237091325	26.971880448372193	26.391785921724054	23.452503392812428
50-51	23.09880831898571	27.03162385842558	26.289224741844986	23.580343080743724
52-53	23.10395158165603	26.94559939887814	26.299375454765645	23.65107356470018
54-55	23.104633667393507	27.139410285889742	26.303177364094022	23.452778682622732
56-57	22.884374356978448	26.970910901338762	26.336692265238316	23.808022476444478
58-59	22.928873029205647	26.69790219417817	26.42588732570021	23.947337450915974
60-61	23.09601300107154	26.947365608680528	26.25791862127949	23.69870276896844
62-63	22.949500997675653	27.010832740271002	26.24327873072348	23.796387531329867
64-65	23.003356531853257	26.64221549745006	26.35307931652703	24.00134865416965
66-67	22.771510212256683	26.766741566402263	26.360212314007036	24.10153590733402
68-69	23.074593138419303	26.719945284024817	26.225866408972504	23.97959516858338
70-71	22.98505181443107	26.4672172781338	26.383043784324627	24.1646871231105
72-73	22.75076407204973	26.666402441441083	26.477321872938077	24.105511613571107
74-75	22.76502594299838	26.51012137837856	26.4276275884676	24.297225090155457
76-77	22.852732173047162	26.516248319876727	26.27373333011648	24.357286176959626
78-79	22.857675096915493	26.600456532688465	26.43121582136205	24.110652549033993
80-81	22.712562555052685	26.44857786487062	26.521826909128617	24.317032670948084
82-83	22.725337223953314	26.423704308095946	26.529279647084557	24.321678820866182
84-85	22.717052030973374	26.407277402232403	26.40437617192631	24.47129439486791
86-87	22.881632419081278	26.459550297155804	26.3916275464489	24.26718973731402
88-89	22.742940379120785	26.297832679030797	26.489161152030395	24.470065789818022
90-91	22.727137902431927	26.229364282022804	26.464886284174682	24.57861153137059
92-93	22.858949373282464	26.29616536043187	26.35432879949625	24.490556466789414
94-95	22.84153334524066	26.017788508439903	26.52423204849695	24.61644609782249
96-97	22.745734522690057	26.075411932010777	26.472738192372386	24.706115352926773
98-99	22.81811622145296	26.14023663471634	26.471250887749044	24.570396256081654
100	22.92765534416466	25.886332616519898	26.40850549931916	24.777506539996285
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10532.0
1	7272.0
2	5093.5
3	5978.5
4	5329.5
5	4517.0
6	3902.0
7	3589.0
8	3495.5
9	3339.0
10	3088.0
11	2849.0
12	2734.0
13	2765.5
14	2842.0
15	2990.5
16	3219.5
17	3569.0
18	4069.0
19	4951.0
20	6436.5
21	8436.5
22	11447.5
23	16147.0
24	22920.5
25	31463.5
26	42354.0
27	55957.5
28	71905.5
29	90377.0
30	107936.5
31	123855.5
32	140123.0
33	155809.5
34	171129.0
35	187230.0
36	206714.5
37	228832.0
38	249970.5
39	266788.5
40	283385.0
41	300024.0
42	310243.5
43	318232.0
44	326642.0
45	333694.5
46	340448.0
47	341919.0
48	342653.5
49	348088.0
50	351628.0
51	352710.5
52	349798.0
53	349216.5
54	349656.0
55	340213.5
56	321712.5
57	297218.5
58	272377.0
59	250987.0
60	228362.5
61	200890.0
62	172214.5
63	144179.0
64	117563.5
65	95960.5
66	76959.5
67	59505.0
68	45714.0
69	34708.5
70	26008.5
71	19818.0
72	15418.5
73	11926.0
74	9450.5
75	6923.5
76	4526.0
77	3162.5
78	2201.5
79	1394.5
80	845.0
81	478.0
82	293.0
83	194.5
84	144.5
85	114.5
86	94.0
87	83.5
88	78.5
89	55.5
90	37.5
91	38.5
92	39.5
93	25.5
94	18.0
95	18.5
96	15.0
97	13.5
98	11.5
99	12.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.136643476297712
2	0.15019324864659195
3	0.14604861239869926
4	0.15206630541246652
5	0.16140170003812865
6	0.0980166620355006
7	0.08912960546549993
8	0.08926908841615018
9	0.0866388384896029
10-11	0.07913664835820099
12-13	0.07567946379565589
14-15	0.0725361158720738
16-17	0.07826986145058881
18-19	0.10819393589901594
20-21	0.07289478631660298
22-23	0.07081748665870484
24-25	0.07031933326352544
26-27	0.06827192280933807
28-29	0.06719092994179875
30-31	0.09319453717016392
32-33	0.09443493912416065
34-35	0.06787838162714632
36-37	0.1109238165045991
38-39	0.06588576804642869
40-41	0.06771897254068893
42-43	0.07957502334595887
44-45	0.09340376159613928
46-47	0.07492725216893498
48-49	0.07383131469954027
50-51	0.06926324806574509
52-53	0.06466529222823914
54-55	0.06168633492506627
56-57	0.06277729086050918
58-59	0.06160663038183757
60-61	0.06180589173990933
62-63	0.06480477517888937
64-65	0.06305127522785786
66-67	0.06524315016664725
68-69	0.07065309603829564
70-71	0.07162947669284728
72-73	0.0771789055151459
74-75	0.06592562031804305
76-77	0.06530791010802059
78-79	0.0665632566638727
80-81	0.06878502080637285
82-83	0.0676591941332674
84-85	0.0681623290623986
86-87	0.06532285470987596
88-89	0.06389813599966285
90-91	0.07193833179785852
92-93	0.06688207483678751
94-95	0.06804277224755553
96-97	0.0674798589110028
98-99	0.060585415921719774
100	0.06077471421188795
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0037069E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.70553516881679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.27321112871611	42.27272369009384
2	12.236327495862193	13.387896883240982
3	4.178670734204426	6.857892564267769
4	1.9099065520898517	4.1792984021801995
5	1.0828658472225663	2.961937784417234
6	0.6621691398665582	2.17345903012051
7	0.47725559017193825	1.8275965730865769
8	0.34303909975293173	1.5012910028650606
9	0.2530022129797622	1.2456559313957571
>10	1.4277759645075518	14.272697919322402
>50	0.10504595118036412	3.9777671769220264
>100	0.04917857779919868	4.588668710875239
>500	0.0013146780113289667	0.47882920835046777
>1k	2.0056210375231885E-4	0.13140281559443012
>5k	3.6465531557966864E-5	0.14288230726760448
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.96306790358819E-6	0.0	0.0	0.0
3	0.0	9.96306790358819E-6	0.0	0.0	0.0
4	0.0	1.992613580717638E-5	0.0	0.0	0.0
5	0.0	2.9889203710764567E-5	0.0	0.0	0.0
6	0.0	2.9889203710764567E-5	0.0	0.0	0.0
7	0.0	2.9889203710764567E-5	0.0	9.96306790358819E-6	0.0
8	0.0	3.985227161435276E-5	0.0	9.96306790358819E-6	0.0
9	9.96306790358819E-6	3.985227161435276E-5	0.0	9.96306790358819E-6	0.0
10-11	9.96306790358819E-6	4.483380556614685E-5	0.0	2.4907669758970473E-5	0.0
12-13	9.96306790358819E-6	5.479687346973504E-5	0.0	5.9778407421529134E-5	0.0
14-15	9.96306790358819E-6	5.9778407421529134E-5	0.0	5.9778407421529134E-5	0.0
16-17	9.96306790358819E-6	5.9778407421529134E-5	0.0	6.475994137332323E-5	0.0
18-19	1.4944601855382285E-5	5.9778407421529134E-5	9.96306790358819E-6	9.963067903588189E-5	0.0
20-21	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	2.4409516363791062E-4	0.0
22-23	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	7.571931606727023E-4	9.96306790358819E-6
24-25	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	0.0019029459695853442	9.96306790358819E-6
26-27	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	0.004179506985555245	9.96306790358819E-6
28-29	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	0.009773769613420014	1.992613580717638E-5
30-31	1.992613580717638E-5	5.9778407421529134E-5	9.96306790358819E-6	0.026143090179015407	1.992613580717638E-5
32-33	1.992613580717638E-5	6.475994137332323E-5	9.96306790358819E-6	0.06207987610725801	1.992613580717638E-5
34-35	2.9889203710764567E-5	8.468607718049962E-5	9.96306790358819E-6	0.1175841273981478	1.992613580717638E-5
36-37	2.9889203710764567E-5	1.0461221298767599E-4	9.96306790358819E-6	0.20444215338162963	1.992613580717638E-5
38-39	2.9889203710764567E-5	1.2453834879485237E-4	9.96306790358819E-6	0.3302059595286233	1.992613580717638E-5
40-41	2.9889203710764567E-5	1.2951988274664646E-4	9.96306790358819E-6	0.47674276225459844	1.992613580717638E-5
42-43	2.9889203710764567E-5	1.5940908645741103E-4	9.96306790358819E-6	0.627209995268539	1.992613580717638E-5
44-45	2.9889203710764567E-5	1.5940908645741103E-4	9.96306790358819E-6	0.8087520370737712	1.992613580717638E-5
46-47	3.985227161435276E-5	1.793352222645874E-4	9.96306790358819E-6	1.0247613122914667	1.992613580717638E-5
48-49	3.985227161435276E-5	1.8929829016817558E-4	9.96306790358819E-6	1.2594015244888723	1.992613580717638E-5
50-51	3.985227161435276E-5	1.9926135807176378E-4	9.96306790358819E-6	1.499117919783156	1.992613580717638E-5
52-53	3.985227161435276E-5	2.1918749387894016E-4	9.96306790358819E-6	1.741359952790999	1.992613580717638E-5
54-55	3.985227161435276E-5	2.2915056178252833E-4	9.96306790358819E-6	2.004609114473558	1.992613580717638E-5
56-57	3.985227161435276E-5	2.4409516363791062E-4	9.96306790358819E-6	2.311645959592387	1.992613580717638E-5
58-59	3.985227161435276E-5	2.4907669758970474E-4	9.96306790358819E-6	2.6384395683640314	2.9889203710764567E-5
60-61	3.985227161435276E-5	2.4907669758970474E-4	1.992613580717638E-5	2.9824941922786423	2.9889203710764567E-5
62-63	3.985227161435276E-5	2.590397654932929E-4	1.992613580717638E-5	3.3266434653383374	2.9889203710764567E-5
64-65	3.985227161435276E-5	2.739843673486752E-4	1.992613580717638E-5	3.6901210901309933	2.9889203710764567E-5
66-67	3.985227161435276E-5	3.038735710594398E-4	1.992613580717638E-5	4.092240473787717	2.9889203710764567E-5
68-69	4.9815339517940946E-5	3.1881817291482206E-4	1.992613580717638E-5	4.5237907600316385	2.9889203710764567E-5
70-71	4.9815339517940946E-5	3.1881817291482206E-4	1.992613580717638E-5	4.973732869625585	2.9889203710764567E-5
72-73	4.9815339517940946E-5	3.237997068666162E-4	1.992613580717638E-5	5.441503889233002	2.9889203710764567E-5
74-75	4.9815339517940946E-5	3.337627747702043E-4	1.992613580717638E-5	5.9054690169012485	3.985227161435276E-5
76-77	4.9815339517940946E-5	3.487073766255866E-4	1.992613580717638E-5	6.395059155217524	4.9815339517940946E-5
78-79	4.9815339517940946E-5	3.487073766255866E-4	1.992613580717638E-5	6.922479062363724	4.9815339517940946E-5
80-81	4.9815339517940946E-5	3.487073766255866E-4	1.992613580717638E-5	7.471354436240301	4.9815339517940946E-5
82-83	4.9815339517940946E-5	3.536889105773807E-4	1.992613580717638E-5	8.036957801127002	5.479687346973504E-5
84-85	4.9815339517940946E-5	3.68633512432763E-4	1.992613580717638E-5	8.585723581256639	5.9778407421529134E-5
86-87	4.9815339517940946E-5	3.68633512432763E-4	2.9889203710764567E-5	9.152397975942977	5.9778407421529134E-5
88	5.9778407421529134E-5	3.68633512432763E-4	2.9889203710764567E-5	9.605563138003735	6.974147532511733E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	10070	0.0	36.14651	2
TATAACG	1100	0.0	35.48463	2
GGTATCA	9035	0.0	31.178381	1
GTACCTG	12515	0.0	30.099312	1
GTATCAA	13330	0.0	29.881897	1
TCAACGC	13530	0.0	28.396873	4
ATCAACG	13815	0.0	27.709484	3
ACCTGGG	13500	0.0	26.82328	3
TAACGCA	1475	0.0	26.781414	4
CAACGCA	15205	0.0	25.330498	5
AACGCAG	15965	0.0	24.227375	6
ATAACGC	1825	0.0	23.964867	3
GTACATG	29510	0.0	23.250965	1
TATCAAC	16590	0.0	23.10288	2
TACATGG	31090	0.0	21.418915	2
GTATAAC	2915	0.0	20.972958	1
ACGCAGA	18370	0.0	20.721802	7
ACATGGG	31230	0.0	20.4495	3
TATCACG	1740	0.0	20.00034	2
CGCAGAG	19805	0.0	18.958788	8
>>END_MODULE