FastQCFastQC Report
Wed 25 May 2016
SRR2049436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10037069
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267060.2660736914332262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244100.2431984875265877No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216470.21567053090897353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157170.15658953824069555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159500.050.0518881
TATAACG14000.041.295182
TACCTGG98950.038.9036562
GTATCAA271100.038.7953721
TAACGCA17550.036.15574
GTACCTG129700.030.5584321
ATAACGC20050.030.4755293
ATCAACG338450.030.441663
TCAACGC340900.030.2503074
TATCAAC354950.029.119262
CAACGCA353950.029.0553135
AACGCAG369400.028.0810016
ACCTGGG134500.028.0268553
GTATAAC32500.026.0395011
ACGCAGA416650.024.8159187
CGCAGAG431850.023.9642268
TATCACG20250.023.2111822
GTGGTAT77100.022.3797451
GCAGAGT477650.021.6467119
GTACATG303650.021.2434161
Produced by FastQC (version 0.11.5)