##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10037069 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.369985301485922 31.0 31.0 34.0 30.0 34.0 2 31.405940419459107 31.0 31.0 34.0 28.0 34.0 3 31.504091682541986 31.0 31.0 34.0 28.0 34.0 4 35.15760328039988 37.0 35.0 37.0 32.0 37.0 5 35.26649502957487 37.0 35.0 37.0 33.0 37.0 6 35.32148927141977 37.0 35.0 37.0 33.0 37.0 7 35.26955847369386 37.0 35.0 37.0 32.0 37.0 8 35.22424464751612 37.0 35.0 37.0 32.0 37.0 9 36.81821167115619 39.0 37.0 39.0 33.0 39.0 10-11 36.702732341483355 39.0 36.0 39.0 32.0 39.0 12-13 36.65513139343767 39.0 35.0 39.0 32.0 39.0 14-15 37.78061847537364 39.0 37.0 41.0 32.0 41.0 16-17 37.783955106814545 39.0 37.0 41.0 32.5 41.0 18-19 37.82733460335881 39.5 37.0 41.0 32.5 41.0 20-21 37.887667505324515 40.0 37.0 41.0 33.0 41.0 22-23 37.8041935848005 40.0 37.0 41.0 32.5 41.0 24-25 37.651860468429575 39.0 37.0 41.0 32.0 41.0 26-27 37.6074243387188 39.0 37.0 41.0 32.0 41.0 28-29 37.55295634612057 39.0 37.0 41.0 32.0 41.0 30-31 37.44630758242272 39.0 36.5 41.0 32.0 41.0 32-33 37.31627624558524 39.0 36.0 41.0 31.5 41.0 34-35 37.20248057475743 39.0 36.0 41.0 31.0 41.0 36-37 37.07401852074545 39.0 36.0 41.0 31.0 41.0 38-39 36.97711164484373 39.0 36.0 41.0 31.0 41.0 40-41 36.8639543077765 39.0 35.0 41.0 31.0 41.0 42-43 36.68277566887305 39.0 35.0 41.0 30.0 41.0 44-45 36.54775956008672 39.0 35.0 40.5 30.0 41.0 46-47 36.33689551202647 39.0 35.0 40.0 30.0 41.0 48-49 36.08756610121939 39.0 35.0 40.0 29.5 41.0 50-51 35.9139623330277 39.0 35.0 40.0 29.0 41.0 52-53 35.78780349123833 38.0 35.0 40.0 29.0 41.0 54-55 35.6176941196678 38.0 34.5 40.0 28.0 41.0 56-57 35.44482946166855 38.0 34.0 40.0 28.0 41.0 58-59 35.21940095260877 38.0 34.0 40.0 28.0 41.0 60-61 35.00298911963243 37.0 34.0 40.0 28.0 41.0 62-63 34.74759738126738 37.0 34.0 40.0 27.5 41.0 64-65 34.49884244095563 36.0 34.0 39.5 27.0 41.0 66-67 34.17014015745035 36.0 33.0 39.0 26.0 41.0 68-69 33.89431481441444 35.5 33.0 39.0 26.0 40.5 70-71 33.62454342995949 35.0 33.0 38.5 26.0 40.0 72-73 33.31242263055081 35.0 33.0 37.5 26.0 40.0 74-75 33.02452996985475 35.0 33.0 37.0 26.0 39.5 76-77 32.738388617234776 35.0 32.5 36.5 26.0 39.0 78-79 32.474157694841 35.0 32.0 36.0 26.0 39.0 80-81 32.163026427336504 35.0 32.0 36.0 25.0 37.5 82-83 31.955315690267746 35.0 32.0 35.0 25.0 37.0 84-85 31.673938178565876 35.0 32.0 35.0 25.0 37.0 86-87 31.50765741472934 35.0 32.0 35.0 24.5 36.0 88-89 31.342482103092046 34.0 32.0 35.0 24.0 36.0 90-91 31.17423801709443 34.0 32.0 35.0 24.0 36.0 92-93 30.92181148699884 34.0 31.5 35.0 23.0 35.0 94-95 30.78392078404562 34.0 31.0 35.0 23.0 35.0 96-97 30.623822751442674 34.0 31.5 35.0 20.5 35.0 98-99 30.4143591620223 34.0 31.0 35.0 19.5 35.0 100 30.279738039062998 34.0 31.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1015054573640981 1101 2 0.17085355576419303 1101 3 0.19019206250201037 1101 4 0.2895754534118993 1101 5 0.2129291080913731 1101 6 0.22311124738352106 1101 7 0.17274272935695478 1101 8 0.1810124032012297 1101 9 0.1846627402013752 1101 10-11 0.30556057976573214 1101 12-13 0.33243659407650483 1101 14-15 0.3324127420162597 1101 16-17 0.36125851322582037 1101 18-19 0.3712324790651991 1101 20-21 0.2839716781211692 1101 22-23 0.29261462525719395 1101 24-25 0.44354181296816364 1101 26-27 0.3297494871780984 1101 28-29 0.2773513026875918 1101 30-31 0.2532297891945703 1101 32-33 0.21642571074306005 1101 34-35 0.2742808892842632 1101 36-37 0.3033560531872652 1101 38-39 0.39134372536636874 1101 40-41 0.30990365315270907 1101 42-43 0.3749945925156055 1101 44-45 0.32447159766406486 1101 46-47 0.29478397166215586 1101 48-49 0.4161316483555382 1101 50-51 0.42499004690459685 1101 52-53 0.42421425711133054 1101 54-55 0.3096792070097223 1101 56-57 0.2906228509936568 1101 58-59 0.33296385784218074 1101 60-61 0.3331111021289459 1101 62-63 0.3426877101750705 1101 64-65 0.30539626123990615 1101 66-67 0.374263032342796 1101 68-69 0.3051611737783517 1101 70-71 0.3796141016928516 1101 72-73 0.28090285191354525 1101 74-75 0.314618415364329 1101 76-77 0.30476031434681516 1101 78-79 0.25685058380955184 1101 80-81 0.28074512901845594 1101 82-83 0.20861026852119124 1101 84-85 0.31310660476249197 1101 86-87 0.31564530638212673 1101 88-89 0.27293319495238677 1101 90-91 0.3452751417575399 1101 92-93 0.41477116639574163 1101 94-95 0.3208885343805363 1101 96-97 0.28141003973477297 1101 98-99 0.2367802142225628 1101 100 0.23895838048253637 1102 1 0.1251915556788994 1102 2 0.16894879125800344 1102 3 0.20188732624406214 1102 4 0.24520598384146552 1102 5 0.19030251796127828 1102 6 0.21738271308706203 1102 7 0.16594280770542014 1102 8 0.20259391086968037 1102 9 0.19073120371561458 1102 10-11 0.26734447776796344 1102 12-13 0.23104505098736183 1102 14-15 0.3922556276821325 1102 16-17 0.34130741813419263 1102 18-19 0.3720155511307581 1102 20-21 0.2686596835905348 1102 22-23 0.24724902678828897 1102 24-25 0.40230718532595233 1102 26-27 0.2009291957796293 1102 28-29 0.2549666421763561 1102 30-31 0.26099169602135674 1102 32-33 0.3239007121315751 1102 34-35 0.34147833293231855 1102 36-37 0.3318539766229165 1102 38-39 0.329875086319241 1102 40-41 0.329697139395293 1102 42-43 0.3542253142296943 1102 44-45 0.300488406277573 1102 46-47 0.36940931827785306 1102 48-49 0.39835338156376565 1102 50-51 0.3640728645539042 1102 52-53 0.2965548025095046 1102 54-55 0.33054344752584086 1102 56-57 0.3234638631365243 1102 58-59 0.34608838495160654 1102 60-61 0.33983310495877816 1102 62-63 0.3201218033997222 1102 64-65 0.3543986570480655 1102 66-67 0.4170876122498086 1102 68-69 0.3521919687421047 1102 70-71 0.35838999344723277 1102 72-73 0.29490552001789894 1102 74-75 0.2717980963094462 1102 76-77 0.2749778827036877 1102 78-79 0.2607605949540641 1102 80-81 0.2862128764022458 1102 82-83 0.190296692168765 1102 84-85 0.33487180566188357 1102 86-87 0.2978068541649037 1102 88-89 0.15213004182507106 1102 90-91 0.22045113131093075 1102 92-93 0.3202063691206867 1102 94-95 0.31559729581925566 1102 96-97 0.28668426334064634 1102 98-99 0.25797379940227927 1102 100 0.27331083566662073 1103 1 0.033056961162351683 1103 2 0.0868464841640133 1103 3 0.10356929037426355 1103 4 0.17842645322097894 1103 5 0.1248890850369122 1103 6 0.07911468580693537 1103 7 0.06628393086100459 1103 8 0.13421907464307736 1103 9 0.12591296925658924 1103 10-11 0.2060747353779533 1103 12-13 0.2315485128799324 1103 14-15 0.22698313344793064 1103 16-17 0.20550630634090084 1103 18-19 0.19491459071053896 1103 20-21 0.22461948068053772 1103 22-23 0.13772802564164977 1103 24-25 0.32684260325378744 1103 26-27 0.1596111460095031 1103 28-29 0.19166711391766 1103 30-31 0.14506928253346274 1103 32-33 0.15717078419418584 1103 34-35 0.14908595963016325 1103 36-37 0.0971269178041183 1103 38-39 0.0873244452925448 1103 40-41 0.08000846678751827 1103 42-43 0.1153912763715681 1103 44-45 0.04069657422822104 1103 46-47 0.10035691322305951 1103 48-49 0.1355518464217269 1103 50-51 0.21894303439390228 1103 52-53 0.16919337810799817 1103 54-55 0.17876934977091707 1103 56-57 0.1572769055191685 1103 58-59 0.10770589004936681 1103 60-61 0.12974362659272032 1103 62-63 0.17867414181303332 1103 64-65 0.09372269216926554 1103 66-67 0.1429144596162999 1103 68-69 0.12382876044584634 1103 70-71 0.14422345197491637 1103 72-73 0.11827766985414456 1103 74-75 0.06344670062540558 1103 76-77 0.18988562673074227 1103 78-79 0.14129162195096967 1103 80-81 0.1673248009662558 1103 82-83 0.1668186299504626 1103 84-85 0.08187350463670384 1103 86-87 0.09392649552170695 1103 88-89 0.09500500895233088 1103 90-91 0.060937050112436 1103 92-93 0.20235856280749687 1103 94-95 0.06923452724575085 1103 96-97 0.13122712560632976 1103 98-99 0.11971197737676675 1103 100 0.06341700904885528 1104 1 0.03288246159577213 1104 2 0.029411626872402508 1104 3 0.09885623286897172 1104 4 0.1607316679647397 1104 5 0.13273450179796953 1104 6 0.11924322185635106 1104 7 0.13101761447276772 1104 8 0.18476199516298664 1104 9 0.20017328920098976 1104 10-11 0.22763574674466724 1104 12-13 0.17575254106805716 1104 14-15 0.10664295178891336 1104 16-17 0.1380696580358034 1104 18-19 0.15978205962424852 1104 20-21 0.13308199638354523 1104 22-23 0.11511853049991316 1104 24-25 0.2380628701635743 1104 26-27 0.14152820505859864 1104 28-29 0.15657279832847593 1104 30-31 0.18358422909779648 1104 32-33 0.21332274294491782 1104 34-35 0.2552359419156076 1104 36-37 0.21668914523770866 1104 38-39 0.26528837358151236 1104 40-41 0.26357877347741265 1104 42-43 0.2736414566578773 1104 44-45 0.21153927276277784 1104 46-47 0.19512823296761184 1104 48-49 0.2755452396435629 1104 50-51 0.23592271425989964 1104 52-53 0.24666862017667768 1104 54-55 0.21013703430110553 1104 56-57 0.17115375346244832 1104 58-59 0.1825390835115428 1104 60-61 0.09631757655929363 1104 62-63 0.22495048810687024 1104 64-65 0.20466885854678196 1104 66-67 0.1933483317124427 1104 68-69 0.15519862852150368 1104 70-71 0.2593329055855733 1104 72-73 0.23708680188769193 1104 74-75 0.17764901879139927 1104 76-77 0.18188766550415636 1104 78-79 0.1746445818837543 1104 80-81 0.23005580523657443 1104 82-83 0.14408578864884447 1104 84-85 0.08477872684455434 1104 86-87 0.06821745182856986 1104 88-89 0.01424329395475965 1104 90-91 -0.041115045463072875 1104 92-93 0.09438535226442824 1104 94-95 0.04961700710271799 1104 96-97 0.018057081438890066 1104 98-99 0.034198715570767035 1104 100 0.02385954952141134 1105 1 -0.07119763672143264 1105 2 0.01805580191000189 1105 3 6.110100191953904E-4 1105 4 0.11829666102832448 1105 5 0.05438405816310876 1105 6 0.05021729977688949 1105 7 0.05752869613345979 1105 8 0.1095860040112484 1105 9 0.08604714938059033 1105 10-11 0.12342460350804174 1105 12-13 0.06136472179340302 1105 14-15 0.08015475590887888 1105 16-17 -0.03451830197340655 1105 18-19 0.07424952998809431 1105 20-21 0.09131416079564048 1105 22-23 0.011714914701599355 1105 24-25 0.18375031177496481 1105 26-27 0.0970903799656 1105 28-29 0.036189989758476315 1105 30-31 0.04377582033577454 1105 32-33 -0.0028768923782180877 1105 34-35 -0.015706612996588376 1105 36-37 0.09591320190944685 1105 38-39 0.12130818658475562 1105 40-41 0.07791588745598688 1105 42-43 0.19377660268730068 1105 44-45 0.1376235407698161 1105 46-47 0.0859314094172916 1105 48-49 0.16733915745210481 1105 50-51 0.10439079144576624 1105 52-53 0.123494626412338 1105 54-55 0.050130549312612516 1105 56-57 0.17322713952928837 1105 58-59 0.10908423621291519 1105 60-61 0.11554244852842288 1105 62-63 0.0974796764828767 1105 64-65 0.0844550235520245 1105 66-67 0.17039983060112007 1105 68-69 0.09621340838573644 1105 70-71 0.09160126810728286 1105 72-73 0.0958835004622145 1105 74-75 0.12375041533928766 1105 76-77 0.14729978647751096 1105 78-79 0.04939280637762522 1105 80-81 0.12985725205200538 1105 82-83 0.038174216861264654 1105 84-85 0.0798813658545825 1105 86-87 4.914138200255991E-4 1105 88-89 -0.027107882718176768 1105 90-91 -0.027390061139762167 1105 92-93 0.0397197354527421 1105 94-95 -0.013583099889103778 1105 96-97 0.0014612425927218453 1105 98-99 -0.01193462713757043 1105 100 -0.11582433943617687 1106 1 -0.17959765194212451 1106 2 -0.10800411063982196 1106 3 -0.10758727193463358 1106 4 0.06725747269528881 1106 5 -0.020031681234598864 1106 6 -0.09244238257073789 1106 7 -0.04592873312203949 1106 8 -0.04553285066190682 1106 9 -0.10336141608439675 1106 10-11 0.06357245004909373 1106 12-13 0.08880778233026376 1106 14-15 0.042396063293082875 1106 16-17 -0.024454776014493973 1106 18-19 0.06731645716934054 1106 20-21 0.0010848255162940745 1106 22-23 -0.034311133424594686 1106 24-25 0.15521957920184803 1106 26-27 0.008535240005123512 1106 28-29 0.008027654861585631 1106 30-31 -0.16905465729077918 1106 32-33 -0.12744640535697016 1106 34-35 -0.19109478359645493 1106 36-37 -0.14580438043868327 1106 38-39 -0.04583686766904549 1106 40-41 -0.08178094455509921 1106 42-43 0.0498779281987467 1106 44-45 0.037571727540587574 1106 46-47 -0.11093361476919483 1106 48-49 -0.027227069534397685 1106 50-51 -0.006243916178320319 1106 52-53 -0.031670220865876786 1106 54-55 -0.1258234391639519 1106 56-57 -0.1795003066001044 1106 58-59 -0.07701810186140534 1106 60-61 -0.047725078429714074 1106 62-63 -0.06817444074610535 1106 64-65 0.02579545310226905 1106 66-67 0.011896428088896016 1106 68-69 0.016932328563754595 1106 70-71 -0.029386303704605155 1106 72-73 -0.06087994926425466 1106 74-75 -0.031530602568025756 1106 76-77 -0.00528835970045094 1106 78-79 -0.06248294914799146 1106 80-81 -0.008756800901807082 1106 82-83 -0.05860247411335706 1106 84-85 -0.1047559404658962 1106 86-87 -0.15930735973200072 1106 88-89 -0.14476439677592268 1106 90-91 -0.06088571088158545 1106 92-93 0.04527093404985649 1106 94-95 0.014513061014227446 1106 96-97 -0.14030885973632223 1106 98-99 -0.11176890595955058 1106 100 -0.1601283991683573 1107 1 -0.12938300057799168 1107 2 -0.09484839317324756 1107 3 -0.10231459356833028 1107 4 0.022381607621397848 1107 5 -0.03249886397704671 1107 6 -0.0492757885082824 1107 7 -0.08618787065891098 1107 8 -0.018380236173044295 1107 9 -0.05006215303826167 1107 10-11 0.033409870983689416 1107 12-13 0.02340494571797791 1107 14-15 0.049262714783935735 1107 16-17 -0.07678797091195122 1107 18-19 -0.06064362348259067 1107 20-21 -0.008743391459383076 1107 22-23 0.006789867604517497 1107 24-25 0.13663617706271936 1107 26-27 -0.028806072795681814 1107 28-29 -0.04516898205985598 1107 30-31 -0.06822232880946899 1107 32-33 -0.10925653183264927 1107 34-35 -0.05551980260965905 1107 36-37 -0.10689961323173947 1107 38-39 -0.10485285320399385 1107 40-41 -0.13757335230710055 1107 42-43 -0.087950521181682 1107 44-45 -0.20250802480577335 1107 46-47 -0.16184213199791486 1107 48-49 -0.11139883553827445 1107 50-51 -0.08254038693659993 1107 52-53 -0.135694917791767 1107 54-55 -0.10275506417977454 1107 56-57 -0.2582786471366063 1107 58-59 -0.29554444319796147 1107 60-61 -0.2178121785267919 1107 62-63 -0.2314625656518956 1107 64-65 -0.17245626254249657 1107 66-67 -0.13476368258941562 1107 68-69 -0.12379077413949346 1107 70-71 -0.15671490820718503 1107 72-73 -0.08134159706653321 1107 74-75 -0.09483035662266559 1107 76-77 -0.14615068024675537 1107 78-79 -0.1346391978572541 1107 80-81 -0.07861019520753132 1107 82-83 -0.16374452282986596 1107 84-85 -0.16560726188696862 1107 86-87 -0.2051941097337746 1107 88-89 -0.2055881869668319 1107 90-91 -0.16496346969776 1107 92-93 -0.10997695774362981 1107 94-95 -0.2645066467394699 1107 96-97 -0.26584152042364906 1107 98-99 -0.24851238916944496 1107 100 -0.24117481136875085 1108 1 -0.19893249205998487 1108 2 -0.12178299265886139 1108 3 -0.15497860936649843 1108 4 -0.026288397045995282 1108 5 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28-29 -0.4182999635835216 2215 30-31 -0.2995766771596564 2215 32-33 -0.21233360853427286 2215 34-35 -0.2035213648485339 2215 36-37 -0.27247804944178 2215 38-39 -0.26366431183382844 2215 40-41 -0.2315112192979072 2215 42-43 -0.15157643757257944 2215 44-45 -0.31717512860090125 2215 46-47 -0.09987606833627893 2215 48-49 -0.02888861874354376 2215 50-51 -0.01781725324458705 2215 52-53 -0.1199380773761689 2215 54-55 -0.12375986679680295 2215 56-57 -0.16710635167892463 2215 58-59 -0.30126963672446294 2215 60-61 -0.2630982717429333 2215 62-63 -0.23124980546785423 2215 64-65 -0.20494596562626555 2215 66-67 -0.10081241695752396 2215 68-69 -0.2170881344239035 2215 70-71 -0.20043483569552478 2215 72-73 -0.20387303813662783 2215 74-75 -0.1532490013026333 2215 76-77 -0.06464021129492181 2215 78-79 -0.03906496506773749 2215 80-81 0.07415695769736175 2215 82-83 0.022913127711358783 2215 84-85 0.1468873800504511 2215 86-87 0.05252171597502908 2215 88-89 0.07772250677845349 2215 90-91 0.011409819314646796 2215 92-93 0.04165419865347886 2215 94-95 0.06887100511418964 2215 96-97 0.10992946750331711 2215 98-99 0.24828262451769234 2215 100 0.29654074479488557 2216 1 -0.0329646154151888 2216 2 -0.06377752323774999 2216 3 -0.08493360352652957 2216 4 -0.07239276192849786 2216 5 -0.03387557088831983 2216 6 -0.07193763510908724 2216 7 -0.09121554337708204 2216 8 -0.02873566532539229 2216 9 -2.205983269903392 2216 10-11 -0.658897886134433 2216 12-13 -0.42600163475920994 2216 14-15 -0.3573503715774464 2216 16-17 -0.5094952259856171 2216 18-19 -0.47006287363586097 2216 20-21 -0.6604237466601717 2216 22-23 -0.16273050511490794 2216 24-25 0.05999412958999528 2216 26-27 -0.07447159906569567 2216 28-29 -0.11116906966830697 2216 30-31 -0.03805456168080923 2216 32-33 -0.059189740874835195 2216 34-35 -0.03814741329281901 2216 36-37 -0.31781673720268344 2216 38-39 -0.2826968538952528 2216 40-41 -0.14411054756418196 2216 42-43 -0.09404704746858528 2216 44-45 -0.15319866568825802 2216 46-47 -0.20859394584162771 2216 48-49 -0.13758464810110382 2216 50-51 -0.2349406087102608 2216 52-53 -0.24110447451627692 2216 54-55 -0.16983131368573368 2216 56-57 -0.14705629273923648 2216 58-59 -0.12716205199283337 2216 60-61 -0.14169497542612675 2216 62-63 -0.12683043287835716 2216 64-65 -0.1235961025911223 2216 66-67 -0.03679610899994401 2216 68-69 -0.10464052042763683 2216 70-71 -0.10128204069689417 2216 72-73 -0.07661122121599107 2216 74-75 -0.09655305781301138 2216 76-77 -0.1309427990852896 2216 78-79 -0.1116465245235787 2216 80-81 -0.03726987668257209 2216 82-83 -0.06636090453461563 2216 84-85 0.047909522698454055 2216 86-87 0.0023828239606231705 2216 88-89 -0.033765831738360674 2216 90-91 -0.009979669826563509 2216 92-93 -0.0604344202392646 2216 94-95 -0.045873789919085084 2216 96-97 -0.07482900541574367 2216 98-99 0.026021981267973615 2216 100 0.01332004910716833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 203.0 3 68.0 4 148.0 5 338.0 6 1091.0 7 2663.0 8 5317.0 9 8082.0 10 11317.0 11 15979.0 12 20561.0 13 25239.0 14 27929.0 15 33621.0 16 42275.0 17 53427.0 18 48591.0 19 33647.0 20 30564.0 21 32206.0 22 36527.0 23 42721.0 24 52777.0 25 68146.0 26 89064.0 27 117804.0 28 155002.0 29 203255.0 30 261657.0 31 330842.0 32 418923.0 33 533861.0 34 701188.0 35 963314.0 36 1412522.0 37 2021454.0 38 1813093.0 39 421159.0 40 494.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.64607169338957 18.788320324023505 13.73676478795755 23.828843194629375 2 16.75303816283419 19.72022908281292 38.40954964043786 25.117183113915026 3 18.66313239951898 25.077458635424833 29.599371124951055 26.660037840105133 4 13.362772221039936 17.028366064020314 36.263592620312075 33.345269094627675 5 14.291416175298574 35.561948048441465 33.68860688589849 16.458028890361472 6 31.366903664893712 35.892507663633495 17.87352827648106 14.867060394991732 7 27.878068786814158 31.220817551418644 21.916836478856528 18.98427718291067 8 26.34072755701889 33.975167451773025 20.130996409409956 19.55310858179813 9 25.59318860914476 16.380817945956135 20.9466030372014 37.0793904076977 10-11 25.018075495944085 25.594110192925847 27.710275778715875 21.677538532414196 12-13 25.656408240037887 23.286417728368914 28.002686641827733 23.054487389765466 14-15 22.99904404325368 24.87762109740248 25.773485575713618 26.34984928363022 16-17 22.55202142219435 27.772176871988602 26.194494865880642 23.48130683993641 18-19 22.56910857143654 26.418165502299523 28.70689142417971 22.305834502084224 20-21 23.357710303675304 25.745772994088213 28.551382878806553 22.345133823429926 22-23 23.12886859699779 25.644772393215586 28.51800161979558 22.708357389991043 24-25 22.919170925297017 25.694109505474156 28.618713291699 22.768006277529825 26-27 22.924794486018275 25.638919082636995 28.601030384486247 22.835256046858483 28-29 22.847239124870615 25.633679527247686 28.71162979810907 22.80745154977263 30-31 22.995284822326628 25.969354259324362 28.401267682538965 22.63409323581004 32-33 22.889426186070853 25.76036888856697 28.536483110756738 22.81372181460544 34-35 22.98340282407145 25.434656272662863 28.57195163249351 23.009989270772174 36-37 23.17906253309607 25.729881900781994 28.38186625996095 22.709189306160994 38-39 23.071242212243433 25.785580431897 28.28161787071505 22.861559485144518 40-41 23.012706499547477 25.535539860843826 28.462116626090406 22.98963701351829 42-43 22.96220754097673 25.735592020718002 28.319180047441144 22.983020390864127 44-45 22.966201587335906 25.89200592324313 28.10705495797628 23.034737531444687 46-47 23.12789719787719 25.815748601509064 27.89189254353039 23.164461657083358 48-49 23.13141378851013 25.86469732642016 27.731642122145843 23.27224676292387 50-51 23.044620505173473 25.95992434841772 27.60143476176855 23.394020384640253 52-53 23.01058705484639 25.984383488845197 27.601454169538936 23.403575286769474 54-55 22.981081429249915 26.23900463372325 27.55653069636166 23.22338324066518 56-57 22.766367352859685 26.005759251032345 27.594943304663943 23.632930091444027 58-59 22.766307574452263 25.752687363213305 27.77728239190146 23.703722670432974 60-61 22.912834414110332 26.03522004282326 27.586489641547747 23.465455901518663 62-63 22.707326212463023 26.145675595136385 27.585119719711003 23.56187847268959 64-65 22.731516541332933 25.813277760668978 27.679171080720877 23.776034617277215 66-67 22.570243372036625 25.954732763319033 27.60978159926551 23.865242265378832 68-69 22.890311338446214 25.9681724766721 27.421091867719333 23.720424317162358 70-71 22.79825913321907 25.78270608680682 27.472980408922165 23.946054371051947 72-73 22.610629656924747 26.007363304964827 27.50364673192941 23.87836030618102 74-75 22.659513449593703 25.89299724849954 27.346593911031196 24.10089539087556 76-77 22.78340419897482 25.92782813389048 27.13649273508033 24.152274932054368 78-79 22.75789355700435 26.09316719906845 27.24761851388292 23.901320730044272 80-81 22.566254152482163 25.978619854062973 27.281440428475683 24.173685564979177 82-83 22.59272779515448 25.996650814092487 27.22993452469078 24.180686866062253 84-85 22.601518123454944 26.0242277060644 27.0267222108062 24.347531959674455 86-87 22.7346752933628 26.09059326137335 26.976669462970232 24.19806198229362 88-89 22.531331001343172 25.951449843871167 27.080639671154817 24.436579483630844 90-91 22.388098587230793 25.947332886954307 27.06494097549776 24.599627550317145 92-93 22.56518567839444 26.013466693779524 26.937980537924506 24.483367089901535 94-95 22.433328718768937 25.779696577529187 27.088241912236967 24.698732791464913 96-97 22.34663888695716 25.8482213221359 27.04320746449509 24.761932326411852 98-99 22.31773765304962 25.97169979048155 27.039656568875948 24.670905987592885 100 22.38684881565893 25.734174296890583 27.022769022556208 24.856207864894277 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2117.0 1 2090.0 2 9716.5 3 13892.5 4 6903.5 5 3030.5 6 3705.5 7 8396.5 8 13722.5 9 15039.5 10 11353.5 11 6844.5 12 5633.0 13 5854.0 14 6446.0 15 7144.5 16 7936.0 17 8812.5 18 9814.5 19 10916.5 20 12297.0 21 14157.5 22 16865.5 23 21304.0 24 27427.0 25 34818.0 26 44630.5 27 57338.5 28 72333.5 29 90175.5 30 106723.0 31 122271.0 32 138803.0 33 152966.5 34 167357.5 35 184084.0 36 203033.5 37 224090.0 38 245577.0 39 262771.0 40 278225.0 41 295119.5 42 306349.0 43 315180.0 44 325999.0 45 333339.5 46 339744.5 47 341104.5 48 340711.5 49 346042.0 50 350094.5 51 351454.5 52 347365.0 53 347087.5 54 346735.0 55 335171.5 56 317847.0 57 292946.0 58 267930.0 59 248803.0 60 227321.0 61 200083.5 62 171386.0 63 142498.0 64 115268.5 65 93569.0 66 74350.0 67 57049.5 68 43943.5 69 33470.0 70 24705.0 71 18323.0 72 13864.0 73 10635.0 74 8633.5 75 6400.5 76 4001.5 77 2733.5 78 1949.0 79 1149.5 80 587.5 81 271.5 82 115.0 83 58.0 84 39.0 85 25.0 86 20.5 87 15.0 88 7.5 89 4.5 90 2.0 91 2.0 92 2.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.032170746260686264 2 0.0 3 9.863437224552308E-4 4 4.981533951794095E-5 5 0.002949068099462104 6 0.0070438890078368495 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 3.4870737662558664E-5 14-15 3.985227161435276E-5 16-17 1.992613580717638E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.885596482399394E-4 28-29 1.5940908645741103E-4 30-31 1.3450141669844057E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 9.96306790358819E-6 42-43 4.981533951794095E-6 44-45 0.0 46-47 0.0 48-49 1.992613580717638E-5 50-51 1.992613580717638E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.023413209573432243 68-69 1.4944601855382285E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 6.475994137332323E-5 80-81 0.0 82-83 2.988920371076457E-5 84-85 6.475994137332323E-5 86-87 8.96676111322937E-5 88-89 5.4796873469735047E-5 90-91 8.46860771804996E-5 92-93 9.96306790358819E-5 94-95 7.472300927691142E-5 96-97 6.974147532511733E-5 98-99 5.4796873469735047E-5 100 1.0959374693947009E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0037069E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.56102886439946 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5544690973931 35.233196680796844 2 14.695025530254172 14.272111178755198 3 4.983061028175427 7.259477112668729 4 2.331839596844541 4.5294611987806945 5 1.3017962943153554 3.160828371190812 6 0.8413436974982157 2.451390934745486 7 0.5812317249171045 1.975764739942294 8 0.41837567018810257 1.6253402396933534 9 0.31953222249218394 1.396513213159379 >10 1.7690798107805654 15.676711363937065 >50 0.1334658448062684 4.466313015773457 >100 0.06781981528457806 5.712929510101117 >500 0.0022615870318469064 0.7234021116076527 >1k 5.5435802074527E-4 0.4164450590353666 >5k 6.159514193820321E-5 0.2179332557012749 >10k+ 8.21268559176043E-5 0.8821820141114832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26706 0.2660736914332262 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24410 0.2431984875265877 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21647 0.21567053090897353 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15717 0.15658953824069555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.992613580717638E-5 0.0 0.0 0.0 4 0.0 1.992613580717638E-5 0.0 0.0 0.0 5 0.0 1.992613580717638E-5 0.0 0.0 0.0 6 0.0 1.992613580717638E-5 0.0 0.0 0.0 7 0.0 1.992613580717638E-5 0.0 1.992613580717638E-5 0.0 8 0.0 1.992613580717638E-5 0.0 1.992613580717638E-5 0.0 9 2.9889203710764567E-5 1.992613580717638E-5 0.0 2.9889203710764567E-5 0.0 10-11 3.4870737662558664E-5 2.4907669758970473E-5 0.0 4.483380556614685E-5 0.0 12-13 3.985227161435276E-5 3.4870737662558664E-5 0.0 7.970454322870552E-5 0.0 14-15 3.985227161435276E-5 4.483380556614685E-5 0.0 9.963067903588189E-5 0.0 16-17 3.985227161435276E-5 4.9815339517940946E-5 0.0 1.1955681484305827E-4 0.0 18-19 4.9815339517940946E-5 5.9778407421529134E-5 0.0 1.6439062040920512E-4 0.0 20-21 5.9778407421529134E-5 5.9778407421529134E-5 0.0 3.038735710594398E-4 0.0 22-23 5.9778407421529134E-5 6.475994137332323E-5 0.0 8.319161699496138E-4 0.0 24-25 5.9778407421529134E-5 7.970454322870552E-5 0.0 0.002037447386283785 0.0 26-27 5.9778407421529134E-5 8.96676111322937E-5 0.0 0.004363823741771626 0.0 28-29 5.9778407421529134E-5 8.96676111322937E-5 0.0 0.010147384659804571 0.0 30-31 6.475994137332323E-5 8.96676111322937E-5 0.0 0.027214119978651138 0.0 32-33 6.974147532511733E-5 9.963067903588189E-5 0.0 0.06457064308315505 0.0 34-35 6.974147532511733E-5 1.0959374693947008E-4 0.0 0.12194296960596764 0.0 36-37 6.974147532511733E-5 1.2453834879485237E-4 0.0 0.21193438044512797 0.0 38-39 6.974147532511733E-5 1.4446448460202874E-4 0.0 0.34183285977211075 0.0 40-41 6.974147532511733E-5 1.4944601855382283E-4 0.0 0.4915279550235233 0.0 42-43 6.974147532511733E-5 1.4944601855382283E-4 0.0 0.6457014492975988 0.0 44-45 6.974147532511733E-5 1.5940908645741103E-4 0.0 0.8314578688260488 0.0 46-47 6.974147532511733E-5 1.5940908645741103E-4 0.0 1.0520999706189127 0.0 48-49 6.974147532511733E-5 1.6439062040920512E-4 0.0 1.2897639739250573 0.0 50-51 6.974147532511733E-5 2.0922442597535196E-4 0.0 1.5319362654575754 0.0 52-53 6.974147532511733E-5 2.1918749387894016E-4 0.0 1.7769330867407607 0.0 54-55 6.974147532511733E-5 2.4409516363791062E-4 0.0 2.0425086247788076 0.0 56-57 6.974147532511733E-5 2.4907669758970474E-4 0.0 2.352240479765557 0.0 58-59 6.974147532511733E-5 2.540582315414988E-4 0.0 2.681579652386568 0.0 60-61 6.974147532511733E-5 2.789659013004693E-4 0.0 3.0291811284748564 0.0 62-63 6.974147532511733E-5 2.839474352522634E-4 0.0 3.3764538233223265 0.0 64-65 6.974147532511733E-5 2.939105031558516E-4 0.0 3.743139555979938 0.0 66-67 6.974147532511733E-5 2.9889203710764566E-4 0.0 4.148008746378052 0.0 68-69 6.974147532511733E-5 2.9889203710764566E-4 0.0 4.5820896518694845 0.0 70-71 6.974147532511733E-5 3.337627747702043E-4 0.0 5.034582306846749 0.0 72-73 6.974147532511733E-5 3.487073766255866E-4 0.0 5.506204052198904 0.0 74-75 6.974147532511733E-5 3.487073766255866E-4 0.0 5.972679872978855 0.0 76-77 6.974147532511733E-5 3.7859658033635116E-4 0.0 6.4647259075333645 0.0 78-79 6.974147532511733E-5 3.7859658033635116E-4 0.0 6.995881965143409 0.0 80-81 6.974147532511733E-5 3.9852271614352757E-4 0.0 7.5488920121999765 0.0 82-83 6.974147532511733E-5 3.9852271614352757E-4 0.0 8.116951273324911 0.0 84-85 6.974147532511733E-5 4.0848578404711574E-4 0.0 8.667859113053822 0.0 86-87 6.974147532511733E-5 4.184488519507039E-4 0.0 9.23909161130605 2.9889203710764567E-5 88 6.974147532511733E-5 4.184488519507039E-4 0.0 9.695011561642149 2.9889203710764567E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15950 0.0 50.051888 1 TATAACG 1400 0.0 41.29518 2 TACCTGG 9895 0.0 38.903656 2 GTATCAA 27110 0.0 38.795372 1 TAACGCA 1755 0.0 36.1557 4 GTACCTG 12970 0.0 30.558432 1 ATAACGC 2005 0.0 30.475529 3 ATCAACG 33845 0.0 30.44166 3 TCAACGC 34090 0.0 30.250307 4 TATCAAC 35495 0.0 29.11926 2 CAACGCA 35395 0.0 29.055313 5 AACGCAG 36940 0.0 28.081001 6 ACCTGGG 13450 0.0 28.026855 3 GTATAAC 3250 0.0 26.039501 1 ACGCAGA 41665 0.0 24.815918 7 CGCAGAG 43185 0.0 23.964226 8 TATCACG 2025 0.0 23.211182 2 GTGGTAT 7710 0.0 22.379745 1 GCAGAGT 47765 0.0 21.646711 9 GTACATG 30365 0.0 21.243416 1 >>END_MODULE