FastQCFastQC Report
Wed 25 May 2016
SRR2049435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068222
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62800.036.7650221
TACCTGG61550.033.6010742
GTATCAA99700.032.7795221
TATAACG8850.030.8044362
GTACCTG70500.030.2816621
TCAACGC104300.030.012354
ATCAACG105650.029.62993
CAACGCA115700.027.1778535
ATAACGC9800.026.858853
TAACGCA9700.026.1656844
AACGCAG122500.025.7453966
TATCAAC124750.024.980522
GTACATG224800.024.0555061
ACCTGGG85950.023.897973
ACGCAGA141250.022.3280247
TACATGG231900.022.2956662
ACATGGG232300.021.022863
CGCAGAG149650.020.8853978
GTATAAC22100.019.5753021
TATCACG11900.018.9593222
Produced by FastQC (version 0.11.5)