##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049435_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7068222
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.312101968500706	31.0	31.0	34.0	27.0	34.0
2	30.38670418105147	31.0	31.0	34.0	27.0	34.0
3	30.615681850400286	31.0	31.0	34.0	27.0	34.0
4	34.20993893513814	37.0	35.0	37.0	30.0	37.0
5	34.248422870702136	37.0	35.0	37.0	30.0	37.0
6	34.15692192463678	37.0	35.0	37.0	30.0	37.0
7	34.1474652324163	37.0	35.0	37.0	30.0	37.0
8	34.160761221138785	37.0	35.0	37.0	30.0	37.0
9	35.73244190688974	39.0	37.0	39.0	30.0	39.0
10-11	35.69629172654736	39.0	37.0	39.0	30.0	39.0
12-13	35.631309189213354	39.0	37.0	39.0	30.0	39.0
14-15	36.79184623799309	40.0	37.0	41.0	30.0	41.0
16-17	36.74779888068032	40.0	37.0	41.0	30.0	41.0
18-19	36.71393442367826	40.0	37.0	41.0	30.0	41.0
20-21	36.76677989174647	40.0	37.0	41.0	30.0	41.0
22-23	36.68955269090303	40.0	37.0	41.0	30.0	41.0
24-25	36.68144379166359	40.0	37.0	41.0	30.0	41.0
26-27	36.67180650239905	40.0	37.0	41.0	30.0	41.0
28-29	36.61428751106007	40.0	37.0	41.0	30.0	41.0
30-31	36.53780172722362	40.0	37.0	41.0	30.0	41.0
32-33	36.509692748756336	40.0	37.0	41.0	30.0	41.0
34-35	36.42615766737377	40.0	36.5	41.0	30.0	41.0
36-37	36.31268330281647	40.0	36.0	41.0	29.5	41.0
38-39	36.20118213604496	40.0	36.0	41.0	29.0	41.0
40-41	36.0726123203261	40.0	36.0	41.0	29.0	41.0
42-43	35.93311259889687	40.0	35.5	41.0	28.0	41.0
44-45	35.73727770576532	39.0	35.0	41.0	27.5	41.0
46-47	35.59151466946001	39.0	35.0	41.0	27.0	41.0
48-49	35.46177673819527	39.0	35.0	41.0	27.0	41.0
50-51	35.30877376234079	39.0	35.0	41.0	27.0	41.0
52-53	35.15327609970372	39.0	35.0	40.0	26.0	41.0
54-55	34.98260467766859	39.0	34.5	40.0	26.0	41.0
56-57	34.793100513820875	38.0	34.0	40.0	26.0	41.0
58-59	34.569770870241484	38.0	34.0	40.0	26.0	41.0
60-61	34.3265592818109	38.0	34.0	40.0	25.0	41.0
62-63	34.047360496034216	37.0	34.0	40.0	24.5	41.0
64-65	33.77764054383125	37.0	33.0	40.0	24.0	41.0
66-67	33.51095813063031	36.0	33.0	39.5	23.0	41.0
68-69	33.19805737567383	36.0	33.0	39.0	23.0	41.0
70-71	32.879010662087296	35.0	33.0	39.0	22.5	40.5
72-73	32.535216069897075	35.0	32.5	38.5	22.0	40.0
74-75	32.223672374749974	35.0	32.0	37.0	21.0	40.0
76-77	31.911236305254704	35.0	32.0	37.0	20.0	39.0
78-79	31.59611200666872	35.0	32.0	36.5	20.0	39.0
80-81	31.285984084823596	35.0	32.0	36.0	18.5	38.5
82-83	31.013658809811012	35.0	32.0	36.0	18.0	37.0
84-85	30.758513739381698	35.0	31.5	35.0	16.0	37.0
86-87	30.49755207179401	35.0	31.0	35.0	10.0	36.5
88-89	30.283195830578045	35.0	31.0	35.0	7.0	36.0
90-91	30.093337829513562	34.0	31.0	35.0	2.0	36.0
92-93	29.882467472017716	34.0	31.0	35.0	2.0	36.0
94-95	29.67255803510416	34.0	31.0	35.0	2.0	35.0
96-97	29.446413822316277	34.0	31.0	35.0	2.0	35.0
98-99	29.243352217856202	34.0	31.0	35.0	2.0	35.0
100	29.099798082176818	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23706595412124543
1101	2	0.2375136019084323
1101	3	0.30472836730994857
1101	4	0.3480768966335077
1101	5	0.29268114002019274
1101	6	0.3565750950599451
1101	7	0.3590300661048431
1101	8	0.3530591091659332
1101	9	0.4753181522720453
1101	10-11	0.5234638790305439
1101	12-13	0.5216466059888489
1101	14-15	0.6630099908751035
1101	16-17	0.5896386097249362
1101	18-19	0.5690581872779745
1101	20-21	0.6159635369033083
1101	22-23	0.6173049585942252
1101	24-25	0.6238622080615599
1101	26-27	0.5920017243529827
1101	28-29	0.5410877014592046
1101	30-31	0.5280597789373473
1101	32-33	0.5513540229916742
1101	34-35	0.5703409478287753
1101	36-37	0.5485659695700349
1101	38-39	0.5794599424323081
1101	40-41	0.586611216124183
1101	42-43	0.503961869626508
1101	44-45	0.4461265746319185
1101	46-47	0.4351107455791521
1101	48-49	0.4368079564056373
1101	50-51	0.442718496775349
1101	52-53	0.4375537175358133
1101	54-55	0.36724345524614677
1101	56-57	0.3692591386687951
1101	58-59	0.39168652014096494
1101	60-61	0.44357550740725316
1101	62-63	0.4105382039486756
1101	64-65	0.45295157080848725
1101	66-67	0.46714030528647754
1101	68-69	0.44509149482374966
1101	70-71	0.4606437082764927
1101	72-73	0.5161536370173891
1101	74-75	0.43506313586852485
1101	76-77	0.43417848337355736
1101	78-79	0.39898899364668594
1101	80-81	0.4254918874164346
1101	82-83	0.3775393409638248
1101	84-85	0.35581034580289383
1101	86-87	0.3487696734438863
1101	88-89	0.36113212197274436
1101	90-91	0.44486665461588615
1101	92-93	0.39325990323189686
1101	94-95	0.37650519672006766
1101	96-97	0.3585052048855957
1101	98-99	0.430892554035772
1101	100	0.41521455758208603
1102	1	0.05705990123961158
1102	2	0.07435669864843675
1102	3	0.1361646036686004
1102	4	0.175819021520347
1102	5	0.10250271366738417
1102	6	0.13463759674618103
1102	7	0.18572498459107578
1102	8	0.2367403578913425
1102	9	0.32045495782890754
1102	10-11	0.3047984382946751
1102	12-13	0.31621955905927734
1102	14-15	0.3877917598096232
1102	16-17	0.352342354363941
1102	18-19	0.3289461376649072
1102	20-21	0.36729927848120525
1102	22-23	0.38669353451155786
1102	24-25	0.39022447244074243
1102	26-27	0.31397661934369836
1102	28-29	0.324617322844432
1102	30-31	0.2853690740586643
1102	32-33	0.28347664872906364
1102	34-35	0.3196693465708904
1102	36-37	0.3460741416215569
1102	38-39	0.3400922512737026
1102	40-41	0.3642216709959598
1102	42-43	0.29216785074192586
1102	44-45	0.23678569521502624
1102	46-47	0.18803418766496804
1102	48-49	0.20287082361566888
1102	50-51	0.24425416314619497
1102	52-53	0.24365901319266925
1102	54-55	0.23846972107294562
1102	56-57	0.1635816319579959
1102	58-59	0.23511618057167993
1102	60-61	0.24129258074700033
1102	62-63	0.19204589873884004
1102	64-65	0.20053504985261839
1102	66-67	0.2565500009535029
1102	68-69	0.2676879990593619
1102	70-71	0.2288644497673289
1102	72-73	0.22027881617064082
1102	74-75	0.2463954437741407
1102	76-77	0.20402860320054828
1102	78-79	0.2288596910436489
1102	80-81	0.21118691611869522
1102	82-83	0.23087499874849726
1102	84-85	0.26977314223023185
1102	86-87	0.23161250766628072
1102	88-89	0.1652366581205449
1102	90-91	0.1835748098053429
1102	92-93	0.12310714926771382
1102	94-95	0.1860454276235899
1102	96-97	0.1708517926136004
1102	98-99	0.1740933424279305
1102	100	0.18764105536076414
1103	1	0.14807157404483107
1103	2	0.1676310975423334
1103	3	0.2503164092455812
1103	4	0.2570910719001489
1103	5	0.24742917901267703
1103	6	0.2318719971150145
1103	7	0.32821321427412187
1103	8	0.31700656308776587
1103	9	0.3941616982871139
1103	10-11	0.42989774128248115
1103	12-13	0.42485822509650006
1103	14-15	0.48328306707021085
1103	16-17	0.4272477129735037
1103	18-19	0.484759236136064
1103	20-21	0.4902196718050007
1103	22-23	0.49240158787254984
1103	24-25	0.4998826164533057
1103	26-27	0.4734551499997508
1103	28-29	0.37058920838236986
1103	30-31	0.39713555940295464
1103	32-33	0.4869530318380839
1103	34-35	0.44181105982323743
1103	36-37	0.4253326646940252
1103	38-39	0.4480822031895215
1103	40-41	0.4681001363530868
1103	42-43	0.4462640926173407
1103	44-45	0.39607805530992835
1103	46-47	0.3422799911261336
1103	48-49	0.2602634976696834
1103	50-51	0.2512881836895744
1103	52-53	0.3075410837620822
1103	54-55	0.21355398326826247
1103	56-57	0.2981066953963989
1103	58-59	0.31039706369269027
1103	60-61	0.3056451918195293
1103	62-63	0.30603394731493694
1103	64-65	0.3297655985321555
1103	66-67	0.2951679449545921
1103	68-69	0.2914512538118359
1103	70-71	0.30599943106524563
1103	72-73	0.2530859016139786
1103	74-75	0.23939105471720978
1103	76-77	0.23420429531407194
1103	78-79	0.20010412710933778
1103	80-81	0.1875259818414854
1103	82-83	0.2070150641785915
1103	84-85	0.18344739903917784
1103	86-87	0.13296426895664482
1103	88-89	0.18199289343259295
1103	90-91	0.18091704531816788
1103	92-93	0.09890000888542616
1103	94-95	0.15350921878387425
1103	96-97	0.10758678500626218
1103	98-99	0.10231528378097465
1103	100	0.07773862229546324
1104	1	0.15793260563451383
1104	2	0.09045372902729198
1104	3	0.17598403209555258
1104	4	0.23847187116992075
1104	5	0.2061744520690567
1104	6	0.22443162515957482
1104	7	0.27622056646819715
1104	8	0.25627171113418257
1104	9	0.3789601728487142
1104	10-11	0.3870785191198962
1104	12-13	0.41893778713859575
1104	14-15	0.48844610317032533
1104	16-17	0.47778100855882144
1104	18-19	0.44995764451146414
1104	20-21	0.4204220101245326
1104	22-23	0.40591602657877246
1104	24-25	0.45601877998847584
1104	26-27	0.4537521911013087
1104	28-29	0.414353004349465
1104	30-31	0.4344433863573869
1104	32-33	0.48652562564232227
1104	34-35	0.45963422415372435
1104	36-37	0.4550352098361543
1104	38-39	0.4919713206465701
1104	40-41	0.4946330747818948
1104	42-43	0.4694053775814595
1104	44-45	0.44992570749474226
1104	46-47	0.3977068441034106
1104	48-49	0.3172021817623687
1104	50-51	0.3247834914448191
1104	52-53	0.3385257178453642
1104	54-55	0.3356504983547879
1104	56-57	0.3364659430740815
1104	58-59	0.4230546656759415
1104	60-61	0.378180581226637
1104	62-63	0.35549939416230814
1104	64-65	0.39617369034619543
1104	66-67	0.43905930528941184
1104	68-69	0.42631405877514794
1104	70-71	0.3882276547501391
1104	72-73	0.36610980943158467
1104	74-75	0.40547317175082753
1104	76-77	0.409743309219067
1104	78-79	0.4171871176984716
1104	80-81	0.41456322883663077
1104	82-83	0.3461523941213187
1104	84-85	0.32862419133280696
1104	86-87	0.38346077653110555
1104	88-89	0.3721619064037611
1104	90-91	0.3803253997211513
1104	92-93	0.35477853410679927
1104	94-95	0.36281660960878526
1104	96-97	0.3934392695092015
1104	98-99	0.3863312384762523
1104	100	0.32532801023505087
1105	1	0.05511524936569501
1105	2	0.05500437767565103
1105	3	0.039201768268213755
1105	4	0.06947321246109794
1105	5	0.10141764431376998
1105	6	0.09733817379027698
1105	7	0.15650195981839232
1105	8	0.20232579733485778
1105	9	0.2560824383270699
1105	10-11	0.2799621201672764
1105	12-13	0.23933704976248293
1105	14-15	0.29285849657401997
1105	16-17	0.27200814054005207
1105	18-19	0.2357834957438385
1105	20-21	0.25097534687179035
1105	22-23	0.18975893425919566
1105	24-25	0.20011542950447136
1105	26-27	0.2560324881989047
1105	28-29	0.21060757495673244
1105	30-31	0.24415302646841042
1105	32-33	0.18583099113122614
1105	34-35	0.1826185766999302
1105	36-37	0.219472157607143
1105	38-39	0.22842334299223666
1105	40-41	0.25290243750944796
1105	42-43	0.2262847629119875
1105	44-45	0.15796248685342817
1105	46-47	0.09370178861760081
1105	48-49	0.1022512073009878
1105	50-51	0.09314637165068262
1105	52-53	0.10697092983419054
1105	54-55	0.09449825674607126
1105	56-57	0.11284839656318013
1105	58-59	0.031063603485698366
1105	60-61	0.07885302954540663
1105	62-63	0.07309470232443971
1105	64-65	0.09694844106586942
1105	66-67	0.056704733433754484
1105	68-69	0.07729239606376836
1105	70-71	0.10015299103966413
1105	72-73	0.05011735470717582
1105	74-75	0.12478746204619995
1105	76-77	0.08951340728626178
1105	78-79	0.039182518080078665
1105	80-81	0.09771935413053257
1105	82-83	0.11310403672972313
1105	84-85	0.16469894721259948
1105	86-87	0.1095971866339589
1105	88-89	0.10311604001987718
1105	90-91	0.12717993336396916
1105	92-93	0.07058960188773611
1105	94-95	0.06206049156520521
1105	96-97	0.05615998983846282
1105	98-99	0.03929694330123823
1105	100	0.03506539517630358
1106	1	0.06857817899343033
1106	2	0.014293377431318532
1106	3	0.1313953329500528
1106	4	0.10798141799522654
1106	5	0.09943452927551988
1106	6	0.09923997209320845
1106	7	0.18825197944678962
1106	8	0.19489000388779232
1106	9	0.23477545144187673
1106	10-11	0.22453875835235948
1106	12-13	0.25055221716087317
1106	14-15	0.3473145452877304
1106	16-17	0.32829063399059777
1106	18-19	0.34527130016177665
1106	20-21	0.3051784135721576
1106	22-23	0.3059127479152721
1106	24-25	0.3314843869500166
1106	26-27	0.33001829044142994
1106	28-29	0.3136643451199035
1106	30-31	0.2991794762578337
1106	32-33	0.28806487005797976
1106	34-35	0.31507764935852123
1106	36-37	0.3218201855213607
1106	38-39	0.31487346165190644
1106	40-41	0.35407363615425425
1106	42-43	0.26845813247249595
1106	44-45	0.1784760675447643
1106	46-47	0.2248731867021263
1106	48-49	0.2611828513006955
1106	50-51	0.2694930770297219
1106	52-53	0.35436191489603885
1106	54-55	0.259223807367043
1106	56-57	0.2389919855168614
1106	58-59	0.30125173224693214
1106	60-61	0.22011508495164378
1106	62-63	0.246607944734329
1106	64-65	0.27658496596658466
1106	66-67	0.27958510817637006
1106	68-69	0.2759701603537579
1106	70-71	0.22062417838579051
1106	72-73	0.25331573664603724
1106	74-75	0.30081355784970754
1106	76-77	0.3498406174372164
1106	78-79	0.27500790596922187
1106	80-81	0.2727694880611402
1106	82-83	0.28905399113236996
1106	84-85	0.2660336019842333
1106	86-87	0.2696937074485568
1106	88-89	0.33417538014357717
1106	90-91	0.31621271541231977
1106	92-93	0.27058910481187937
1106	94-95	0.2910069284902015
1106	96-97	0.3145931320430826
1106	98-99	0.2952508739625337
1106	100	0.29477307992466706
1107	1	0.23714037243649955
1107	2	0.18282443561431094
1107	3	0.20448318823097011
1107	4	0.2535102317458424
1107	5	0.24564624933479706
1107	6	0.28738809975625657
1107	7	0.23434914723183908
1107	8	0.2627870016064122
1107	9	0.36692421853645385
1107	10-11	0.3061990203994043
1107	12-13	0.34893886511628125
1107	14-15	0.3763499839093569
1107	16-17	0.3883227418748092
1107	18-19	0.42329149029619373
1107	20-21	0.3565179989443905
1107	22-23	0.3752593096209651
1107	24-25	0.3741799655746192
1107	26-27	0.40977306773064015
1107	28-29	0.3982436596552219
1107	30-31	0.38764663216282
1107	32-33	0.39889660898124646
1107	34-35	0.41685717698181435
1107	36-37	0.42198852115683394
1107	38-39	0.40187469922076957
1107	40-41	0.47658451384867817
1107	42-43	0.42794394438417527
1107	44-45	0.3923614041550252
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2216	100	-1.5116785713300374
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166460.0
3	27058.0
4	17861.0
5	14287.0
6	12733.0
7	12768.0
8	13884.0
9	15740.0
10	17244.0
11	18630.0
12	20315.0
13	22079.0
14	23046.0
15	24151.0
16	24938.0
17	26263.0
18	25405.0
19	24236.0
20	24407.0
21	26303.0
22	29413.0
23	34459.0
24	41218.0
25	50025.0
26	60926.0
27	72560.0
28	88219.0
29	108412.0
30	131124.0
31	160720.0
32	203229.0
33	267268.0
34	370631.0
35	545344.0
36	910779.0
37	1504833.0
38	1541853.0
39	388968.0
40	433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.348308357658325	18.6680354062273	13.228385623874727	23.75527061223964
2	16.59313965142577	19.678189538811072	38.28047958565828	25.44819122410488
3	18.59721777851613	24.946768685510936	29.50211262553244	26.9539009104405
4	13.248624636923816	16.778219688206228	37.128428396431154	32.8447272784388
5	14.08141913088365	36.33457976880429	34.02064160532361	15.563359494988457
6	31.35428098580429	36.11568309051217	18.176849005632214	14.353186918051325
7	28.24212256015451	31.21749867971125	21.92395363225161	18.61642512788263
8	26.48456882141932	34.293639885821065	20.146344645432183	19.075446647327432
9	25.68710600227164	17.103479327779084	20.635657333000715	36.57375733694857
10-11	24.800837473841327	26.17298906305916	27.91019117536561	21.115982287733903
12-13	25.28896020738967	23.660535256668773	27.865772638317566	23.18473189762399
14-15	22.813913563952564	25.57767949023172	25.055409672876515	26.552997272939198
16-17	22.892165345913025	28.65546038934769	25.3769075672441	23.075466697495177
18-19	22.920616844710036	27.90268751719212	26.946282200908083	22.230413437189764
20-21	23.761970432698703	27.138015926427578	26.624056388315488	22.475957252558228
22-23	23.594667570022263	27.08975093973957	26.496718649941855	22.818862840296312
24-25	23.23964015695959	27.198793759284907	26.557976610584433	23.003589473171072
26-27	23.12092198604139	27.156376065270436	26.560329049081798	23.162372899606368
28-29	23.148402654656568	27.113980388007537	26.657913372242053	23.079703585093846
30-31	23.2499838513872	27.486346905030807	26.36490091755958	22.898768326022413
32-33	22.966098583082125	27.313426954006147	26.639009733107716	23.081464729804008
34-35	23.147410522049984	26.9586496295005	26.71394223694309	23.179997611506426
36-37	23.4475296256446	27.12627709918004	26.46971468708629	22.95647858808907
38-39	23.224768883096523	27.33143996479297	26.429712584391456	23.014078567719057
40-41	23.17266545182553	27.08247665713604	26.633017038811996	23.111840852226432
42-43	23.094319658136225	27.170086226492746	26.623304266668008	23.11228984870302
44-45	23.07566419512557	27.290375961320617	26.49654131886814	23.13741852468567
46-47	23.211944552971456	27.21126362752982	26.42472540464617	23.152066414852552
48-49	23.131415822980365	27.110336005927387	26.579350119632434	23.17889805145981
50-51	23.105189794088403	27.148412820484157	26.486032081632803	23.260365303794636
52-53	23.147419623664415	27.09519848556209	26.47646482297421	23.280917067799283
54-55	23.138696537317973	27.35809937191685	26.475379883297872	23.027824207467308
56-57	22.925265301507576	27.18010106737575	26.502910593820005	23.391723037296668
58-59	23.001165323064455	26.85242646409628	26.677005442324713	23.469402770514552
60-61	23.185282324790137	27.143773632480343	26.453506758342847	23.21743728438667
62-63	23.021747040789737	27.256834496692818	26.522756633101796	23.198661829415652
64-65	23.093539142574546	26.965983753026645	26.568501707714525	23.37197539668428
66-67	22.917103696877614	27.04628658451383	26.537542737574928	23.499066981033625
68-69	23.20034192615476	27.134452512748297	26.466880042699735	23.19832551839721
70-71	23.179352256054486	26.86213223640118	26.591432302515983	23.367083205028347
72-73	22.962994670870348	27.101058184586563	26.6649921746981	23.270954969844997
74-75	22.974718576236068	26.998207601910316	26.62863383719014	23.398439984663476
76-77	23.089828732202008	27.062574626636003	26.405644446214733	23.441952194947255
78-79	23.159289249240324	27.132432690572966	26.568048760643396	23.14022929954331
80-81	22.978122300310538	27.028528531715622	26.643339227102565	23.35000994087127
82-83	22.98816427803794	27.040708685873334	26.668890566443547	23.302236469645184
84-85	23.028124885252957	27.030600839048073	26.487335711416815	23.45393856428215
86-87	23.188227890928804	27.130942858028273	26.440103145548683	23.240726105494243
88-89	22.99292705682884	27.054741675093748	26.572549899699325	23.37978136837809
90-91	22.936366534601934	26.977182220037417	26.589704611115145	23.496746634245504
92-93	23.12079778388648	27.16939323952371	26.40543402014411	23.3043749564457
94-95	23.145062517365147	26.906703048193375	26.544446679415675	23.4037877550258
96-97	23.09102423845565	26.939360847765837	26.50731958687036	23.462295326908155
98-99	23.040239215497902	27.018134929272946	26.48975748289471	23.451868372334438
100	23.182894239410697	26.878227272418577	26.42855970553014	23.510318782640585
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2714.0
1	2138.0
2	2150.0
3	2602.0
4	2231.0
5	1805.0
6	1536.5
7	1486.0
8	1507.0
9	1491.5
10	1364.5
11	1228.0
12	1266.0
13	1371.5
14	1450.0
15	1557.5
16	1706.5
17	1961.0
18	2407.0
19	3018.0
20	3921.5
21	5194.5
22	7288.5
23	10638.0
24	15226.0
25	21234.0
26	29095.5
27	39528.0
28	52620.5
29	66902.0
30	80884.5
31	94981.0
32	109060.0
33	122153.0
34	134730.5
35	148623.5
36	163579.0
37	177887.0
38	193161.5
39	205734.0
40	217359.0
41	228330.5
42	233587.5
43	236964.0
44	241123.0
45	244755.0
46	246805.5
47	244798.0
48	241266.5
49	241475.5
50	242965.0
51	241849.0
52	235634.0
53	231710.5
54	230184.5
55	222150.5
56	209066.5
57	192204.0
58	175451.5
59	162063.5
60	147060.0
61	128902.0
62	110416.0
63	93294.0
64	76760.5
65	62781.5
66	50603.0
67	39424.5
68	30267.5
69	22628.0
70	16876.5
71	12589.0
72	9688.0
73	7434.5
74	5753.0
75	4273.5
76	2928.0
77	2094.0
78	1662.0
79	1252.5
80	850.5
81	577.5
82	414.0
83	330.5
84	273.5
85	212.5
86	164.5
87	139.5
88	114.0
89	104.0
90	95.0
91	74.0
92	61.0
93	49.5
94	40.0
95	34.5
96	24.5
97	17.5
98	17.5
99	27.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053973969691387735
2	0.017048134594527448
3	0.020132361434035323
4	0.01668029102651275
5	0.01607193435633459
6	0.014402490470729414
7	0.02243845764889671
8	0.015124029777219787
9	0.015293803731688111
10-11	0.017755526071478796
12-13	0.018844908945983868
14-15	0.015414060282769839
16-17	0.016567108390200536
18-19	0.014572264425197738
20-21	0.017345239014847018
22-23	0.017507939054545826
24-25	0.01849828712227771
26-27	0.011693181114005757
28-29	0.01653173881635297
30-31	0.014876442760286816
32-33	0.01637611269142367
34-35	0.014232716516261092
36-37	0.014628855743353843
38-39	0.0205426484906671
40-41	0.016694438856051774
42-43	0.013617285931313419
44-45	0.02137737043346969
46-47	0.019368378638927868
48-49	0.019347156894619327
50-51	0.015039142799985625
52-53	0.014791555783052655
54-55	0.015343321135074703
56-57	0.020528500661128074
58-59	0.009252680518523612
60-61	0.012492533482960779
62-63	0.017833339133943443
64-65	0.02282752296121995
66-67	0.015697016873550376
68-69	0.01696324761729329
70-71	0.024419153784360482
72-73	0.019835257013715753
74-75	0.020266765814656074
76-77	0.01353239895407926
78-79	0.014812777527361196
80-81	0.0203304310475817
82-83	0.014954255822751466
84-85	0.014805703612591682
86-87	0.018604395843820412
88-89	0.017267425952382367
90-91	0.017444273821620202
92-93	0.012655233522659588
94-95	0.017904078281638577
96-97	0.008630176018806427
98-99	0.0064018928664096855
100	0.004725375066034995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7068222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.524937503242654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49067400350381	44.00121241632464
2	13.476982153823199	15.505251122619923
3	4.16544393126446	7.1885070545774825
4	1.8341240356268707	4.220314820905237
5	1.042333522547122	2.9980085371028977
6	0.66159042959538	2.2834768869130615
7	0.44545743077001165	1.7937437605779971
8	0.30667229173785915	1.4113043532957237
9	0.22956828907217683	1.1885311333442985
>10	1.234078402360495	12.759788044347545
>50	0.07833962632772823	3.0868320962949025
>100	0.033996292626263795	3.227902103048645
>500	6.409910045616872E-4	0.238094892013889
>1k	9.859974019207165E-5	0.09703277863386989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.4147829539026929E-5	0.0
7	0.0	0.0	0.0	2.8295659078053858E-5	0.0
8	0.0	0.0	0.0	2.8295659078053858E-5	0.0
9	0.0	0.0	0.0	5.6591318156107715E-5	0.0
10-11	0.0	0.0	0.0	6.366523292562118E-5	0.0
12-13	0.0	7.0739147695134644E-6	0.0	9.90348067731885E-5	0.0
14-15	0.0	2.122174430854039E-5	0.0	9.90348067731885E-5	0.0
16-17	0.0	2.8295659078053858E-5	0.0	1.1318263631221543E-4	0.0
18-19	0.0	4.244348861708079E-5	0.0	1.5562612492929623E-4	0.0
20-21	0.0	4.951740338659425E-5	0.0	1.98069613546377E-4	0.0
22-23	0.0	5.6591318156107715E-5	0.0	4.1736097140129445E-4	0.0
24-25	0.0	5.6591318156107715E-5	0.0	9.620524086538312E-4	0.0
26-27	0.0	5.6591318156107715E-5	0.0	0.001506743845906368	0.0
28-29	0.0	7.78130624646481E-5	0.0	0.003239852964437167	0.0
30-31	1.4147829539026929E-5	9.90348067731885E-5	0.0	0.008311849854178321	0.0
32-33	1.4147829539026929E-5	9.90348067731885E-5	0.0	0.018958091582296086	0.0
34-35	1.4147829539026929E-5	9.90348067731885E-5	0.0	0.03494513896139652	0.0
36-37	1.4147829539026929E-5	1.0610872154270196E-4	0.0	0.06155013240953666	0.0
38-39	1.4147829539026929E-5	1.202565510817289E-4	0.0	0.10014541139200211	1.4147829539026929E-5
40-41	1.4147829539026929E-5	1.2733046585124236E-4	0.0	0.14592071386552374	1.4147829539026929E-5
42-43	1.4147829539026929E-5	1.2733046585124236E-4	0.0	0.19409407344591045	1.4147829539026929E-5
44-45	1.4147829539026929E-5	1.2733046585124236E-4	0.0	0.25077735249402183	1.4147829539026929E-5
46-47	1.4147829539026929E-5	1.3440438062075583E-4	0.0	0.3203493042521868	1.4147829539026929E-5
48-49	2.8295659078053858E-5	1.414782953902693E-4	0.0	0.3983533624156117	1.4147829539026929E-5
50-51	2.8295659078053858E-5	1.5562612492929623E-4	0.0	0.47890402989606157	1.4147829539026929E-5
52-53	2.8295659078053858E-5	1.5562612492929623E-4	0.0	0.5600984236205371	1.4147829539026929E-5
54-55	2.8295659078053858E-5	1.627000396988097E-4	0.0	0.649371228011797	1.4147829539026929E-5
56-57	3.5369573847567324E-5	1.6977395446832316E-4	0.0	0.7555860582760416	1.4147829539026929E-5
58-59	4.951740338659425E-5	1.6977395446832316E-4	0.0	0.8728786390693444	1.4147829539026929E-5
60-61	5.6591318156107715E-5	1.6977395446832316E-4	0.0	0.9952361428376189	2.122174430854039E-5
62-63	5.6591318156107715E-5	1.6977395446832316E-4	0.0	1.1206566517010925	2.8295659078053858E-5
64-65	5.6591318156107715E-5	1.6977395446832316E-4	0.0	1.255952345582807	2.8295659078053858E-5
66-67	5.6591318156107715E-5	1.6977395446832316E-4	0.0	1.4097746222458776	2.8295659078053858E-5
68-69	5.6591318156107715E-5	1.6977395446832316E-4	0.0	1.5764431281303841	2.8295659078053858E-5
70-71	6.366523292562118E-5	1.839217840073501E-4	0.0	1.7572948331277654	2.8295659078053858E-5
72-73	7.073914769513465E-5	1.98069613546377E-4	0.0	1.9525702503401847	2.8295659078053858E-5
74-75	7.073914769513465E-5	1.98069613546377E-4	0.0	2.1492675244212758	2.8295659078053858E-5
76-77	7.073914769513465E-5	1.98069613546377E-4	0.0	2.357523575235752	2.8295659078053858E-5
78-79	7.073914769513465E-5	1.98069613546377E-4	0.0	2.5910476496069306	2.8295659078053858E-5
80-81	7.073914769513465E-5	1.98069613546377E-4	0.0	2.8421504021803505	2.8295659078053858E-5
82-83	7.073914769513465E-5	1.98069613546377E-4	0.0	3.1020531047270445	2.8295659078053858E-5
84-85	7.073914769513465E-5	1.98069613546377E-4	0.0	3.362967377085779	2.8295659078053858E-5
86-87	7.073914769513465E-5	1.98069613546377E-4	0.0	3.6354757957517467	2.8295659078053858E-5
88	7.073914769513465E-5	1.98069613546377E-4	0.0	3.854731784032816	2.8295659078053858E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6280	0.0	36.765022	1
TACCTGG	6155	0.0	33.601074	2
GTATCAA	9970	0.0	32.779522	1
TATAACG	885	0.0	30.804436	2
GTACCTG	7050	0.0	30.281662	1
TCAACGC	10430	0.0	30.01235	4
ATCAACG	10565	0.0	29.6299	3
CAACGCA	11570	0.0	27.177853	5
ATAACGC	980	0.0	26.85885	3
TAACGCA	970	0.0	26.165684	4
AACGCAG	12250	0.0	25.745396	6
TATCAAC	12475	0.0	24.98052	2
GTACATG	22480	0.0	24.055506	1
ACCTGGG	8595	0.0	23.89797	3
ACGCAGA	14125	0.0	22.328024	7
TACATGG	23190	0.0	22.295666	2
ACATGGG	23230	0.0	21.02286	3
CGCAGAG	14965	0.0	20.885397	8
GTATAAC	2210	0.0	19.575302	1
TATCACG	1190	0.0	18.959322	2
>>END_MODULE