FastQCFastQC Report
Wed 25 May 2016
SRR2049435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068222
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153370.21698526164005602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135520.19173138591289293No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94190.13325840642809464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75720.1071273652695119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95450.046.733491
GTATCAA167150.039.382851
TATAACG10100.037.69522
TACCTGG64100.033.5104752
ATCAACG196850.033.022443
TCAACGC197150.032.9245074
TAACGCA12400.032.5986184
GTACCTG70650.031.302681
CAACGCA209800.031.0961325
TATCAAC212250.030.6707532
ATAACGC13800.030.3133093
AACGCAG223550.029.607986
ACGCAGA253050.026.137777
CGCAGAG262650.025.2003178
GTATAAC21950.024.6523951
ACCTGGG87300.023.9589733
GTACATG228150.022.8927691
TATCACG11750.022.8012892
GCAGAGT301850.021.9432329
TACATGG234050.021.6286532
Produced by FastQC (version 0.11.5)