##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049435_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7068222
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.853729127353386	33.0	31.0	34.0	30.0	34.0
2	32.0261088290662	33.0	31.0	34.0	30.0	34.0
3	31.97859008955859	33.0	31.0	34.0	30.0	34.0
4	35.724348075088756	37.0	35.0	37.0	33.0	37.0
5	35.76487538167307	37.0	35.0	37.0	35.0	37.0
6	35.79800973993177	37.0	35.0	37.0	35.0	37.0
7	35.80228351627892	37.0	35.0	37.0	35.0	37.0
8	35.804526088739145	37.0	35.0	37.0	35.0	37.0
9	37.53223427334343	39.0	37.0	39.0	35.0	39.0
10-11	37.49248361186166	39.0	37.0	39.0	35.0	39.0
12-13	37.46002679315958	39.0	37.0	39.0	34.5	39.0
14-15	38.76438558381443	40.0	38.0	41.0	35.0	41.0
16-17	38.76677366670147	40.0	38.0	41.0	35.0	41.0
18-19	38.81420348710043	40.0	38.0	41.0	35.0	41.0
20-21	38.82090248438716	40.0	38.0	41.0	35.0	41.0
22-23	38.78768974715282	40.0	38.0	41.0	35.0	41.0
24-25	38.7601343732554	40.0	38.0	41.0	35.0	41.0
26-27	38.65923120411328	40.0	38.0	41.0	34.0	41.0
28-29	38.580072824537766	40.0	38.0	41.0	34.0	41.0
30-31	38.49563751676164	40.0	38.0	41.0	34.0	41.0
32-33	38.38825301469026	40.0	38.0	41.0	34.0	41.0
34-35	38.28875882789194	40.0	38.0	41.0	34.0	41.0
36-37	38.183929282357006	40.0	38.0	41.0	34.0	41.0
38-39	38.10237553376224	40.0	38.0	41.0	33.0	41.0
40-41	38.02505142028646	40.0	38.0	41.0	33.0	41.0
42-43	37.90690006624014	40.0	37.0	41.0	33.0	41.0
44-45	37.75968029866634	40.0	37.0	41.0	33.0	41.0
46-47	37.56453560739887	40.0	37.0	41.0	33.0	41.0
48-49	37.36953982203728	40.0	37.0	41.0	32.0	41.0
50-51	37.213443069558366	40.0	36.0	41.0	32.0	41.0
52-53	37.07462265616445	40.0	36.0	41.0	32.0	41.0
54-55	36.9085200634615	39.0	36.0	41.0	31.0	41.0
56-57	36.73042103091838	39.0	35.0	41.0	31.0	41.0
58-59	36.514229108819734	39.0	35.0	41.0	31.0	41.0
60-61	36.28762289865825	38.5	35.0	40.5	31.0	41.0
62-63	36.03708521039662	38.0	35.0	40.0	30.5	41.0
64-65	35.78022450341825	37.5	35.0	40.0	30.0	41.0
66-67	35.485092361275576	37.0	35.0	40.0	30.0	41.0
68-69	35.16566400998724	36.5	34.5	39.5	30.0	41.0
70-71	34.84945924165936	36.0	34.0	39.0	29.0	41.0
72-73	34.519043685951004	35.5	34.0	39.0	29.0	40.5
74-75	34.20072247306324	35.0	34.0	37.5	29.0	40.0
76-77	33.892794892407174	35.0	34.0	37.0	29.0	39.0
78-79	33.5982932482879	35.0	34.0	37.0	29.0	39.0
80-81	33.31347812787997	35.0	34.0	36.0	29.0	39.0
82-83	33.067735846440584	35.0	33.5	36.0	29.0	37.0
84-85	32.82518404204056	35.0	33.0	35.5	29.0	37.0
86-87	32.61767874580057	35.0	33.0	35.0	28.0	37.0
88-89	32.42830361015825	35.0	33.0	35.0	27.5	36.0
90-91	32.26164684697227	35.0	33.0	35.0	27.0	36.0
92-93	32.108393313056666	35.0	33.0	35.0	27.0	36.0
94-95	31.958846298262845	35.0	33.0	35.0	27.0	35.5
96-97	31.798837812394687	35.0	33.0	35.0	26.5	35.0
98-99	31.65781302568029	35.0	33.0	35.0	26.0	35.0
100	31.54264184118722	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26171580201235045
1101	2	0.26597495067382937
1101	3	0.4045741528010822
1101	4	0.24381169514582268
1101	5	0.21662610160166196
1101	6	0.21194502637150237
1101	7	0.15869898617942368
1101	8	0.2014059071273806
1101	9	0.3101576086179918
1101	10-11	0.29799833712485935
1101	12-13	0.2449599063849277
1101	14-15	0.4141958292653456
1101	16-17	0.35507225278249166
1101	18-19	0.3678310233798783
1101	20-21	0.36321010144637
1101	22-23	0.3897115808433469
1101	24-25	0.3884391136524883
1101	26-27	0.4426147836626555
1101	28-29	0.39822244360485826
1101	30-31	0.3765146893701825
1101	32-33	0.3947021819918106
1101	34-35	0.38445180181084737
1101	36-37	0.4217957678309361
1101	38-39	0.418944553040717
1101	40-41	0.4611195247981499
1101	42-43	0.4597234971774924
1101	44-45	0.44371688596059045
1101	46-47	0.423129386986524
1101	48-49	0.44954590712135456
1101	50-51	0.3966552656624316
1101	52-53	0.40451307138328474
1101	54-55	0.4051799171675654
1101	56-57	0.430794920473609
1101	58-59	0.4249435075657786
1101	60-61	0.37411031413768114
1101	62-63	0.36420887324342033
1101	64-65	0.32431696111967057
1101	66-67	0.344294216335129
1101	68-69	0.37725902725218674
1101	70-71	0.38313473898688954
1101	72-73	0.3661337393806079
1101	74-75	0.38565692895528514
1101	76-77	0.39120120730480323
1101	78-79	0.37269048590946596
1101	80-81	0.348184960888247
1101	82-83	0.36004946606731636
1101	84-85	0.33355538275846897
1101	86-87	0.26832184653095226
1101	88-89	0.33786085429534296
1101	90-91	0.3433135839106072
1101	92-93	0.34337389392425166
1101	94-95	0.33359433284120854
1101	96-97	0.3043017761440332
1101	98-99	0.4091275250979223
1101	100	0.43267747964592473
1102	1	0.22573778224858998
1102	2	0.21361534665476967
1102	3	0.31194924190845086
1102	4	0.18259072452141112
1102	5	0.1983381967530491
1102	6	0.14883291061688908
1102	7	0.15615008516388684
1102	8	0.17223577315081684
1102	9	0.17024864966202102
1102	10-11	0.18753765769948672
1102	12-13	0.2132672046997115
1102	14-15	0.27991949802444793
1102	16-17	0.28420517206833296
1102	18-19	0.2913138920074161
1102	20-21	0.2885537642778928
1102	22-23	0.26877667446088793
1102	24-25	0.25280469282618157
1102	26-27	0.25840554020056317
1102	28-29	0.2909009917044614
1102	30-31	0.2732938196437118
1102	32-33	0.28366069304140495
1102	34-35	0.2824609053583558
1102	36-37	0.3163443884484636
1102	38-39	0.2941708760290851
1102	40-41	0.3407038360463872
1102	42-43	0.3372654556175476
1102	44-45	0.3476736473071256
1102	46-47	0.2889258204006566
1102	48-49	0.21390621863920245
1102	50-51	0.25374891904409935
1102	52-53	0.2799085321675747
1102	54-55	0.21294530009248547
1102	56-57	0.24528449541276132
1102	58-59	0.2942414205328632
1102	60-61	0.23535033464226274
1102	62-63	0.2773197454255225
1102	64-65	0.24778554929829966
1102	66-67	0.24069108604084022
1102	68-69	0.23538459720843008
1102	70-71	0.21096303385266424
1102	72-73	0.21904763166482866
1102	74-75	0.18525156807079668
1102	76-77	0.16960430664751414
1102	78-79	0.1882364858955654
1102	80-81	0.1909793004564193
1102	82-83	0.15799964088497376
1102	84-85	0.11016774762039461
1102	86-87	0.12730619256340958
1102	88-89	0.16698348887348402
1102	90-91	0.11333275700439316
1102	92-93	0.07820836795816177
1102	94-95	0.05457628415376803
1102	96-97	0.10777522782986892
1102	98-99	0.19732046883972743
1102	100	0.19400037150484906
1103	1	0.2140124270617072
1103	2	0.22912804052004532
1103	3	0.2987166433186488
1103	4	0.18794960914032544
1103	5	0.18821056631425392
1103	6	0.1521905398456056
1103	7	0.15509955712233392
1103	8	0.17883601604629717
1103	9	0.19180814796527557
1103	10-11	0.18962846153833368
1103	12-13	0.2077927467044205
1103	14-15	0.2819423639144958
1103	16-17	0.27657346780506487
1103	18-19	0.263730695285723
1103	20-21	0.2675389188550952
1103	22-23	0.2786652862314227
1103	24-25	0.27746769742197586
1103	26-27	0.303609642283142
1103	28-29	0.34589440497413904
1103	30-31	0.3317806610997849
1103	32-33	0.299388549391189
1103	34-35	0.33187912704330813
1103	36-37	0.32713043705599176
1103	38-39	0.3417288202998492
1103	40-41	0.32771462727400547
1103	42-43	0.3800735380098885
1103	44-45	0.39329082539055094
1103	46-47	0.40265059019017
1103	48-49	0.4017414855400361
1103	50-51	0.3991330182210362
1103	52-53	0.3472932348612474
1103	54-55	0.3474228867918896
1103	56-57	0.29828268345843156
1103	58-59	0.29721551868977514
1103	60-61	0.31402764875507927
1103	62-63	0.2772420457244493
1103	64-65	0.3207510649995555
1103	66-67	0.33196976921873755
1103	68-69	0.34478247270875073
1103	70-71	0.3543947777669132
1103	72-73	0.4149652014666927
1103	74-75	0.35527187270785987
1103	76-77	0.2684295023629488
1103	78-79	0.2806418291652264
1103	80-81	0.28624846293481454
1103	82-83	0.27996723429104975
1103	84-85	0.286709830861426
1103	86-87	0.26613256776781213
1103	88-89	0.27550436470289696
1103	90-91	0.33057529780904815
1103	92-93	0.3404701723066026
1103	94-95	0.331900318374565
1103	96-97	0.3353676352216617
1103	98-99	0.3563524017338082
1103	100	0.36400864466202876
1104	1	0.21545186934338645
1104	2	0.17303170427106807
1104	3	0.28862411933216947
1104	4	0.1524771359180832
1104	5	0.1672135076882384
1104	6	0.12945979782200112
1104	7	0.1585982241935966
1104	8	0.18800310755092653
1104	9	0.16941643052950184
1104	10-11	0.1921072952359708
1104	12-13	0.1834171751734175
1104	14-15	0.26433593084469464
1104	16-17	0.2806945195669499
1104	18-19	0.2285622792423041
1104	20-21	0.2793003494844086
1104	22-23	0.27765605605782895
1104	24-25	0.2677925690991785
1104	26-27	0.2338061743088602
1104	28-29	0.2646431944035541
1104	30-31	0.31942041359128126
1104	32-33	0.3259718645956724
1104	34-35	0.3313976861993666
1104	36-37	0.3349742214156848
1104	38-39	0.3304260868819284
1104	40-41	0.34403771198326183
1104	42-43	0.33751595096558873
1104	44-45	0.3604167473642619
1104	46-47	0.370798688427989
1104	48-49	0.3544336926639957
1104	50-51	0.3595766646298557
1104	52-53	0.3559557622114795
1104	54-55	0.31907911626728946
1104	56-57	0.3082582388688664
1104	58-59	0.33953437167578926
1104	60-61	0.31978246380248976
1104	62-63	0.32804287532622567
1104	64-65	0.28125868250514685
1104	66-67	0.3281289938342269
1104	68-69	0.33448506156013735
1104	70-71	0.3087754958782156
1104	72-73	0.3286888982611771
1104	74-75	0.3213597367685068
1104	76-77	0.26924101295242764
1104	78-79	0.24641089343180056
1104	80-81	0.29627840489003177
1104	82-83	0.28968139535457027
1104	84-85	0.31968341228012775
1104	86-87	0.3143400120698985
1104	88-89	0.32038186709051075
1104	90-91	0.3258767189119638
1104	92-93	0.3810689547369819
1104	94-95	0.329305729218099
1104	96-97	0.3445742965723504
1104	98-99	0.3342302926523075
1104	100	0.2889964032907457
1105	1	0.20694094721541134
1105	2	0.20347568442203112
1105	3	0.28018582577436746
1105	4	0.13485977320851106
1105	5	0.15877172151115815
1105	6	0.086221986073717
1105	7	0.12118654917691174
1105	8	0.10472408146226542
1105	9	0.18736475025991695
1105	10-11	0.13155799362817788
1105	12-13	0.1642034033564812
1105	14-15	0.24235649710645646
1105	16-17	0.21924234005341248
1105	18-19	0.20690614562879972
1105	20-21	0.1771110915383005
1105	22-23	0.13489679225683204
1105	24-25	0.17499684951724248
1105	26-27	0.1548272834669504
1105	28-29	0.18663702489757128
1105	30-31	0.1709153567352928
1105	32-33	0.18929933617950212
1105	34-35	0.1804363955794699
1105	36-37	0.15820292656218982
1105	38-39	0.17864246353013868
1105	40-41	0.15572110289316754
1105	42-43	0.1828427314406511
1105	44-45	0.15227113261553882
1105	46-47	0.14518458162766734
1105	48-49	0.1885610670204656
1105	50-51	0.14382316237900739
1105	52-53	0.11988503090375247
1105	54-55	0.14902719552639354
1105	56-57	0.12471261935942124
1105	58-59	0.12747658003889484
1105	60-61	0.08838407796932302
1105	62-63	0.09218198774922115
1105	64-65	0.08753459874768765
1105	66-67	0.08956603239541039
1105	68-69	0.0769368530705421
1105	70-71	0.130836296003622
1105	72-73	0.16981184127185855
1105	74-75	0.15426750638308562
1105	76-77	0.1345593939467875
1105	78-79	0.1398896636708784
1105	80-81	0.14136710962853272
1105	82-83	0.11018393204394528
1105	84-85	0.0937530846256962
1105	86-87	0.10684883357544095
1105	88-89	0.10819621424393944
1105	90-91	0.09329794866034291
1105	92-93	0.10110725157604605
1105	94-95	0.05968906137857033
1105	96-97	0.09495820707475033
1105	98-99	0.07665786419131493
1105	100	0.09777007068947086
1106	1	0.13918886361662075
1106	2	0.11621052675753418
1106	3	0.24917020743328777
1106	4	0.1603383669413887
1106	5	0.15269987461936552
1106	6	0.1150372820258454
1106	7	0.0867548444704056
1106	8	0.09410136145498882
1106	9	0.1402768239043084
1106	10-11	0.13381867895928679
1106	12-13	0.1292840811621545
1106	14-15	0.1749801628114369
1106	16-17	0.2015777396292293
1106	18-19	0.1994983616663646
1106	20-21	0.19265022258162645
1106	22-23	0.216813030656958
1106	24-25	0.20571623763374447
1106	26-27	0.20952837055618545
1106	28-29	0.23757378089155168
1106	30-31	0.23564193284727253
1106	32-33	0.21172827811923867
1106	34-35	0.22862302933500445
1106	36-37	0.2223795069541623
1106	38-39	0.26742391148913214
1106	40-41	0.2722332769033571
1106	42-43	0.2524967134539722
1106	44-45	0.25813217233474717
1106	46-47	0.2691691333457342
1106	48-49	0.25656100517760905
1106	50-51	0.26874786648589577
1106	52-53	0.27185547573083824
1106	54-55	0.2853381316401027
1106	56-57	0.2016889030867688
1106	58-59	0.19382203036668244
1106	60-61	0.2157529589121978
1106	62-63	0.1553662227873005
1106	64-65	0.11696794454448423
1106	66-67	0.17295269691916815
1106	68-69	0.17530109655682224
1106	70-71	0.2520200864243236
1106	72-73	0.2563689922900281
1106	74-75	0.25848309443300366
1106	76-77	0.24441366340576565
1106	78-79	0.2562219110196935
1106	80-81	0.23921327993717512
1106	82-83	0.26887239190907763
1106	84-85	0.28407667952913584
1106	86-87	0.23501404135928539
1106	88-89	0.16279658035815459
1106	90-91	0.19731737020628515
1106	92-93	0.24762376877566084
1106	94-95	0.22217197722520865
1106	96-97	0.22732517706127808
1106	98-99	0.26713378107058716
1106	100	0.2406261204025597
1107	1	0.14296621799704567
1107	2	0.11572023833770828
1107	3	0.22369581821990892
1107	4	0.16031628750126004
1107	5	0.14443475003826478
1107	6	0.10828884908674752
1107	7	0.13316411737007172
1107	8	0.12965918812011523
1107	9	0.0822514395431142
1107	10-11	0.13836956198254313
1107	12-13	0.12488957992101746
1107	14-15	0.13570701712546906
1107	16-17	0.15469450700491194
1107	18-19	0.1576601528429009
1107	20-21	0.16556231115291098
1107	22-23	0.14546226378421068
1107	24-25	0.14987922337470394
1107	26-27	0.18131051256647623
1107	28-29	0.18757167342523928
1107	30-31	0.20573389457055669
1107	32-33	0.1969461998417401
1107	34-35	0.19427578792974032
1107	36-37	0.18477090905256688
1107	38-39	0.15766164010273087
1107	40-41	0.15165701822412814
1107	42-43	0.12625570162250455
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2216	68-69	-0.715811464277202
2216	70-71	-0.7290260627658824
2216	72-73	-0.764878074353895
2216	74-75	-0.782020350083755
2216	76-77	-0.7083964557936397
2216	78-79	-0.7058009699222652
2216	80-81	-0.8145730917012344
2216	82-83	-0.7374752911859517
2216	84-85	-0.8665959394697396
2216	86-87	-0.785268118979296
2216	88-89	-0.8913801871324338
2216	90-91	-0.8011409721229583
2216	92-93	-0.8159358937997396
2216	94-95	-0.7854808553946313
2216	96-97	-0.8006050055619589
2216	98-99	-0.9510224316935592
2216	100	-0.9316790999163054
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	389.0
3	46.0
4	65.0
5	149.0
6	351.0
7	1056.0
8	2297.0
9	3623.0
10	5045.0
11	6665.0
12	9253.0
13	12435.0
14	13248.0
15	16110.0
16	20879.0
17	28994.0
18	28705.0
19	18697.0
20	15807.0
21	16594.0
22	17997.0
23	20227.0
24	24268.0
25	30336.0
26	39546.0
27	51517.0
28	67642.0
29	89005.0
30	115581.0
31	149192.0
32	192296.0
33	252990.0
34	349820.0
35	516375.0
36	870622.0
37	1600916.0
38	1901243.0
39	576951.0
40	1290.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.422593220300584	18.95001268842883	13.629496745191425	23.99789734607916
2	16.518100308677344	19.953759233934644	37.90594013600592	25.622200321382095
3	18.240301450633552	24.998903543210726	29.815376483647515	26.94541852250821
4	13.371057106016195	17.09040830919006	36.63287599059565	32.90565859419809
5	13.846934441264668	36.16400281510547	34.131385171683796	15.857677571946066
6	31.36798509043233	36.34904607430478	17.81887444535132	14.464094389911567
7	27.994480082827057	31.403965523437154	21.815486270804737	18.786068122931056
8	26.476762938524917	34.085503648517665	20.06030378541224	19.37742962754518
9	25.661502991841513	16.471794462596108	20.765943684281563	37.100758861280816
10-11	24.78073693780416	25.93477539330259	27.901373216630716	21.383114452262536
12-13	25.492133087049822	23.420044787223897	28.099186183370655	22.98863594235562
14-15	22.813548133257296	25.140569317229893	25.677493672028646	26.36838887748417
16-17	22.485640660409363	28.279813508970147	26.05778369722966	23.17676213339083
18-19	22.47747187888778	27.049916304971482	28.337757949436078	22.13485386670466
20-21	23.310382480340348	26.2893646695607	28.179013116386663	22.221239733712288
22-23	23.046333292870543	26.222252215620845	28.17946295404983	22.551951537458784
24-25	22.808930479743804	26.307887536898434	28.172996300836967	22.710185682520798
26-27	22.753371941353556	26.26557136976697	28.196531220269655	22.784525468609814
28-29	22.73450388354796	26.22487616922208	28.309115406726832	22.731504540503124
30-31	22.91098100767067	26.556049031849877	28.00253019783476	22.530439762644694
32-33	22.63603622327182	26.391664321073264	28.270214057474313	22.702085398180603
34-35	22.801308646029273	26.082070291656816	28.257759041648594	22.85886202066531
36-37	23.10109736955716	26.296166057637844	27.985417263086234	22.617319309718763
38-39	22.924500740285914	26.462890560063606	27.932052151780894	22.680556547869582
40-41	22.833259293004463	26.239512918386808	28.10888738607457	22.818340402534158
42-43	22.792393014161217	26.3612438984759	27.989010235754762	22.857352851608116
44-45	22.833112744872643	26.518279367563096	27.778208501014756	22.870399386549504
46-47	23.00389468608117	26.50494562245193	27.568141090108888	22.92301860135801
48-49	22.980887365446726	26.50218499635834	27.530501383378297	22.986426254816642
50-51	22.977698738435897	26.542370699229366	27.405418061998727	23.074512500336013
52-53	22.988632833906923	26.554189792222456	27.35152761834912	23.1056497555215
54-55	22.97559128107646	26.854153497376704	27.304486622642333	22.865768598904506
56-57	22.723183675184455	26.653182344578546	27.37354875708604	23.250085223150958
58-59	22.801054975800835	26.299835927551197	27.575036936461643	23.324072160186322
60-61	22.96944193988385	26.595809098673683	27.395911868580153	23.038837092862316
62-63	22.7963911354316	26.665937108656912	27.484277659403418	23.053394096508075
64-65	22.865476950501986	26.381521495003156	27.533955720770987	23.21904583372387
66-67	22.714420978206242	26.486241001500737	27.49320989772138	23.30612812257165
68-69	22.990844541121753	26.539095065036122	27.34906933354555	23.120991060296575
70-71	22.985350045336777	26.35600398573161	27.369343566754427	23.289302402177185
72-73	22.75140995909638	26.591722095076097	27.42588054401109	23.23098740181643
74-75	22.771113459815822	26.456918265665575	27.34093418570517	23.431034088813437
76-77	22.9058678730261	26.538747406786317	27.15245312868176	23.402931591505823
78-79	22.937994755058753	26.66265005353598	27.266486081777618	23.13286910962765
80-81	22.760784794202042	26.500676415003312	27.314631022053028	23.42390776874162
82-83	22.769147100474658	26.530731347476227	27.32305857863685	23.377062973412265
84-85	22.794920754212992	26.531316035797843	27.13089419897976	23.542869011009408
86-87	22.91933517421595	26.63339543099396	27.07174101349466	23.375528381295435
88-89	22.708157854284817	26.56565864497952	27.171149345058605	23.555034155677053
90-91	22.597055033941796	26.492406972198722	27.200628341672882	23.709909652186596
92-93	22.750686017606164	26.658837649090472	27.016908739692052	23.573567593611312
94-95	22.72941100903465	26.421459618269182	27.156520501485403	23.69260887121077
96-97	22.64704078477495	26.51315615880923	27.102613136415215	23.737189920000603
98-99	22.546551046738013	26.55891589551423	27.112593608948142	23.781939448799612
100	22.665747996126303	26.462635032897413	27.027192978112485	23.8444239928638
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1243.0
1	994.5
2	4161.5
3	6051.5
4	2871.0
5	1196.5
6	1780.5
7	4015.5
8	7020.0
9	8084.0
10	5871.5
11	3378.5
12	2850.5
13	3092.5
14	3511.5
15	3923.0
16	4339.5
17	4814.5
18	5416.0
19	6071.5
20	6943.0
21	8236.5
22	10211.5
23	13282.5
24	17343.5
25	22643.0
26	30009.5
27	39930.5
28	52429.0
29	66435.5
30	79373.0
31	92827.0
32	107521.0
33	120535.0
34	132736.5
35	146112.5
36	160849.5
37	175960.0
38	190777.0
39	202658.5
40	214288.0
41	225546.5
42	232008.5
43	236328.5
44	239887.5
45	242992.5
46	246003.5
47	243968.5
48	240584.5
49	241523.0
50	242975.0
51	241906.5
52	235899.5
53	231645.0
54	229353.5
55	221510.5
56	209232.0
57	192260.5
58	175108.5
59	162839.5
60	148699.5
61	129163.0
62	109748.0
63	91892.5
64	74654.5
65	61170.0
66	48881.0
67	37353.5
68	28293.5
69	20741.5
70	15207.0
71	11138.5
72	8317.5
73	6165.5
74	4635.0
75	3328.5
76	2118.0
77	1456.0
78	1040.5
79	664.5
80	388.5
81	177.5
82	86.0
83	50.0
84	27.5
85	16.5
86	16.5
87	14.0
88	10.5
89	9.5
90	5.5
91	1.5
92	0.0
93	1.0
94	1.0
95	0.0
96	0.5
97	0.5
98	0.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09436602302530961
2	0.0
3	0.0
4	0.0
5	0.008998019586821128
6	1.2733046585124236E-4
7	0.0
8	1.2733046585124236E-4
9	0.0
10-11	0.0
12-13	0.014543968766119684
14-15	1.4147829539026929E-5
16-17	0.0
18-19	7.0739147695134644E-6
20-21	3.5369573847567324E-5
22-23	0.0
24-25	2.1221744308540395E-5
26-27	2.1221744308540395E-5
28-29	1.0610872154270198E-4
30-31	0.0
32-33	4.951740338659425E-5
34-35	7.0739147695134644E-6
36-37	2.1221744308540395E-5
38-39	9.90348067731885E-5
40-41	2.8295659078053858E-5
42-43	1.202565510817289E-4
44-45	9.90348067731885E-5
46-47	2.1221744308540395E-5
48-49	2.546609317024847E-4
50-51	1.6977395446832316E-4
52-53	1.9099569877686353E-4
54-55	1.414782953902693E-4
56-57	1.9099569877686353E-4
58-59	4.244348861708079E-5
60-61	7.073914769513465E-5
62-63	2.192913578549174E-4
64-65	1.3440438062075583E-4
66-67	4.173609714012944E-4
68-69	4.881001190964291E-4
70-71	1.2733046585124236E-4
72-73	2.9003050555005206E-4
74-75	3.3247399416713285E-4
76-77	1.9099569877686353E-4
78-79	0.0011035307040441005
80-81	0.0010752350449660466
82-83	7.993523689550215E-4
84-85	0.0012167133403563157
86-87	7.710567098769676E-4
88-89	9.974219825013987E-4
90-91	0.0013157481471295044
92-93	0.0010257176415794523
94-95	0.0014147829539026928
96-97	0.001506743845906368
98-99	0.0017118873742222583
100	5.234696929439964E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7068222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.61526532848292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43888709120141	40.65499569242947
2	14.68866968810113	16.044511846761754
3	4.512159265459324	7.392983264623008
4	1.9743240260541444	4.313129221093829
5	1.1372414986755408	3.1055373096363112
6	0.6982685328611861	2.2881672715646504
7	0.4646511290875914	1.7763931290208579
8	0.3400488937680051	1.4857488446237361
9	0.2575978108151751	1.2661895507136418
>10	1.3562758695173394	13.389941696953628
>50	0.09137652762494997	3.42373281550692
>100	0.03914899112735535	3.5861358820383797
>500	9.870672258118984E-4	0.34723742782722533
>1k	2.597203447278129E-4	0.2745022262120829
>5k	5.194406812245202E-5	0.24101215807579954
>10k+	5.194406812245202E-5	0.4097816629187083
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15337	0.21698526164005602	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13552	0.19173138591289293	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9419	0.13325840642809464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7572	0.1071273652695119	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.4147829539026929E-5	0.0	0.0	0.0
6	0.0	1.4147829539026929E-5	0.0	0.0	0.0
7	0.0	1.4147829539026929E-5	0.0	2.8295659078053858E-5	0.0
8	0.0	1.4147829539026929E-5	0.0	2.8295659078053858E-5	0.0
9	0.0	1.4147829539026929E-5	0.0	2.8295659078053858E-5	0.0
10-11	0.0	1.4147829539026929E-5	0.0	2.8295659078053858E-5	0.0
12-13	0.0	1.4147829539026929E-5	0.0	4.951740338659425E-5	0.0
14-15	0.0	1.4147829539026929E-5	0.0	5.6591318156107715E-5	0.0
16-17	0.0	1.4147829539026929E-5	0.0	7.781306246464811E-5	0.0
18-19	7.0739147695134644E-6	4.9517403386594256E-5	0.0	9.196089200367503E-5	0.0
20-21	1.4147829539026929E-5	8.488697723416158E-5	0.0	1.4855221015978273E-4	0.0
22-23	1.4147829539026929E-5	8.488697723416158E-5	0.0	4.102870566317809E-4	0.0
24-25	1.4147829539026929E-5	9.196089200367503E-5	0.0	9.620524086538312E-4	0.0
26-27	1.4147829539026929E-5	9.90348067731885E-5	0.0	0.0015279655902149082	0.0
28-29	1.4147829539026929E-5	9.90348067731885E-5	0.0	0.003239852964437167	0.0
30-31	1.4147829539026929E-5	1.0610872154270196E-4	0.0	0.008311849854178321	0.0
32-33	1.4147829539026929E-5	1.1318263631221543E-4	0.0	0.019014682900452193	0.0
34-35	1.4147829539026929E-5	1.1318263631221543E-4	0.0	0.035001730279552626	0.0
36-37	1.4147829539026929E-5	1.2733046585124236E-4	0.0	0.06172698027877449	0.0
38-39	1.4147829539026929E-5	1.5562612492929623E-4	0.0	0.10043544189755217	0.0
40-41	1.4147829539026929E-5	1.6977395446832316E-4	0.0	0.1464583313880068	0.0
42-43	1.4147829539026929E-5	1.839217840073501E-4	0.0	0.1945892474797764	0.0
44-45	1.4147829539026929E-5	1.9099569877686353E-4	0.0	0.2514988918005122	0.0
46-47	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.32160138716639064	0.0
48-49	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.3998247366876705	0.0
50-51	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.480729099906596	0.0
52-53	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.5624186676649375	0.0
54-55	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.6521017591128292	0.0
56-57	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.758868354729096	0.0
58-59	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.8762811920734804	0.0
60-61	1.4147829539026929E-5	1.98069613546377E-4	0.0	0.9988579871996097	0.0
62-63	1.4147829539026929E-5	1.98069613546377E-4	0.0	1.1248302614151056	0.0
64-65	1.4147829539026929E-5	2.1221744308540393E-4	0.0	1.2605999075863774	0.0
66-67	1.4147829539026929E-5	2.2636527262443086E-4	0.0	1.4149668756867002	0.0
68-69	1.4147829539026929E-5	2.546609317024847E-4	0.0	1.5821234816903034	0.0
70-71	1.4147829539026929E-5	2.546609317024847E-4	0.0	1.763668430335097	0.0
72-73	1.4147829539026929E-5	2.617348464719982E-4	0.0	1.9591560649906017	0.0
74-75	1.4147829539026929E-5	2.6880876124151166E-4	0.0	2.1562353304692468	7.0739147695134644E-6
76-77	1.4147829539026929E-5	2.6880876124151166E-4	0.0	2.365347324970834	1.4147829539026929E-5
78-79	1.4147829539026929E-5	2.6880876124151166E-4	0.0	2.5996636777961983	1.4147829539026929E-5
80-81	1.4147829539026929E-5	2.6880876124151166E-4	0.0	2.852103400261056	1.4147829539026929E-5
82-83	1.4147829539026929E-5	2.758826760110251E-4	0.0	3.113378442273036	1.4147829539026929E-5
84-85	1.4147829539026929E-5	2.829565907805386E-4	0.0	3.3749647365348743	1.4147829539026929E-5
86-87	1.4147829539026929E-5	2.829565907805386E-4	0.0	3.6489586773024394	1.4147829539026929E-5
88	1.4147829539026929E-5	2.829565907805386E-4	0.0	3.869304048458014	1.4147829539026929E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9545	0.0	46.73349	1
GTATCAA	16715	0.0	39.38285	1
TATAACG	1010	0.0	37.6952	2
TACCTGG	6410	0.0	33.510475	2
ATCAACG	19685	0.0	33.02244	3
TCAACGC	19715	0.0	32.924507	4
TAACGCA	1240	0.0	32.598618	4
GTACCTG	7065	0.0	31.30268	1
CAACGCA	20980	0.0	31.096132	5
TATCAAC	21225	0.0	30.670753	2
ATAACGC	1380	0.0	30.313309	3
AACGCAG	22355	0.0	29.60798	6
ACGCAGA	25305	0.0	26.13777	7
CGCAGAG	26265	0.0	25.200317	8
GTATAAC	2195	0.0	24.652395	1
ACCTGGG	8730	0.0	23.958973	3
GTACATG	22815	0.0	22.892769	1
TATCACG	1175	0.0	22.801289	2
GCAGAGT	30185	0.0	21.943232	9
TACATGG	23405	0.0	21.628653	2
>>END_MODULE