FastQCFastQC Report
Wed 25 May 2016
SRR2049434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506399
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG9650.042.375392
GGTATCA72300.033.883561
ATAACGC13300.032.1596643
GTATCAA114600.031.2651121
TCAACGC119900.029.2448964
TAACGCA13050.029.17464
TACCTGG67700.028.951372
ATCAACG121750.028.722733
GTACCTG74900.026.8062271
CAACGCA131450.026.7828885
AACGCAG141150.025.3747436
TATCAAC138550.024.9685332
GTACATG251450.023.91711
TACATGG256800.022.6227382
GTATAAC26450.022.221531
ACGCAGA164750.021.8825267
ACATGGG258450.021.5144353
TATCACG13700.020.928162
CGCAGAG172150.020.8311488
ACCTGGG96200.019.9345663
Produced by FastQC (version 0.11.5)