FastQCFastQC Report
Wed 25 May 2016
SRR2049434_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049434_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506399
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178400.23766389183415376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158280.21086009416765614No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116810.15561389688984026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94980.12653204286103098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110450.048.7302471
TATAACG10300.047.458262
TAACGCA13150.038.602354
GTATCAA185550.037.6026541
ATAACGC14400.036.557063
TACCTGG70100.033.6590162
GTACCTG75850.032.0060771
ATCAACG223200.030.766053
TCAACGC224100.030.7054124
TATCACG14100.030.334582
CAACGCA235450.029.3250545
TATCAAC241600.028.5202122
AACGCAG248150.028.2101946
GTATAAC23400.026.9418431
ACGCAGA283900.024.7073527
ACCTGGG96350.024.049773
CGCAGAG295500.023.6738138
GTACATG244350.023.4708961
TACATGG252400.021.9925862
TCACGCA21250.021.2338124
Produced by FastQC (version 0.11.5)