##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049434_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7506399 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72401347170594 31.0 31.0 34.0 30.0 34.0 2 31.999840402834966 33.0 31.0 34.0 30.0 34.0 3 31.992746188951585 33.0 31.0 34.0 30.0 34.0 4 35.7107860906408 37.0 35.0 37.0 33.0 37.0 5 35.78881991218426 37.0 35.0 37.0 35.0 37.0 6 35.84998439331562 37.0 35.0 37.0 35.0 37.0 7 35.81770606651738 37.0 35.0 37.0 35.0 37.0 8 35.813587180750716 37.0 35.0 37.0 35.0 37.0 9 37.53423205987318 39.0 37.0 39.0 35.0 39.0 10-11 37.491780146512326 39.0 37.0 39.0 35.0 39.0 12-13 37.464484168773865 39.0 37.0 39.0 35.0 39.0 14-15 38.75561943882813 40.0 38.0 41.0 35.0 41.0 16-17 38.75691653214811 40.0 38.0 41.0 35.0 41.0 18-19 38.80767382602497 40.0 38.0 41.0 35.0 41.0 20-21 38.814544164252396 40.0 38.0 41.0 35.0 41.0 22-23 38.77657555906634 40.0 38.0 41.0 35.0 41.0 24-25 38.74911125827444 40.0 38.0 41.0 34.5 41.0 26-27 38.648190763640464 40.0 38.0 41.0 34.0 41.0 28-29 38.56624474664883 40.0 38.0 41.0 34.0 41.0 30-31 38.47700908251746 40.0 38.0 41.0 34.0 41.0 32-33 38.363145897253794 40.0 38.0 41.0 34.0 41.0 34-35 38.25763132228916 40.0 38.0 41.0 34.0 41.0 36-37 38.14886472195256 40.0 38.0 41.0 33.5 41.0 38-39 38.064142740080825 40.0 38.0 41.0 33.0 41.0 40-41 37.983348207309525 40.0 37.5 41.0 33.0 41.0 42-43 37.85858918504066 40.0 37.0 41.0 33.0 41.0 44-45 37.700668522949556 40.0 37.0 41.0 33.0 41.0 46-47 37.49133266163976 40.0 37.0 41.0 33.0 41.0 48-49 37.28589247653902 40.0 37.0 41.0 32.0 41.0 50-51 37.12513250361458 40.0 36.0 41.0 32.0 41.0 52-53 36.98395968559625 39.5 36.0 41.0 31.5 41.0 54-55 36.81469250435528 39.0 35.5 41.0 31.0 41.0 56-57 36.63139862402731 39.0 35.0 41.0 31.0 41.0 58-59 36.414557033272544 39.0 35.0 41.0 31.0 41.0 60-61 36.19185790683389 38.5 35.0 40.5 31.0 41.0 62-63 35.93910082584206 38.0 35.0 40.0 30.5 41.0 64-65 35.685229029258906 37.5 35.0 40.0 30.0 41.0 66-67 35.389641158163855 37.0 35.0 40.0 30.0 41.0 68-69 35.074767608276616 36.5 34.0 39.0 29.5 41.0 70-71 34.76037258344514 36.0 34.0 39.0 29.0 41.0 72-73 34.429498485225736 35.5 34.0 39.0 29.0 40.5 74-75 34.11577948361125 35.0 34.0 37.5 29.0 40.0 76-77 33.81134222947648 35.0 34.0 37.0 29.0 39.0 78-79 33.51904794829052 35.0 34.0 37.0 29.0 39.0 80-81 33.240259810329825 35.0 34.0 36.0 29.0 39.0 82-83 32.99624953323158 35.0 33.5 36.0 29.0 37.0 84-85 32.75678184706142 35.0 33.0 35.5 28.0 37.0 86-87 32.55133766537057 35.0 33.0 35.0 27.5 36.5 88-89 32.363979386121095 35.0 33.0 35.0 27.0 36.0 90-91 32.19871885307455 35.0 33.0 35.0 27.0 36.0 92-93 32.04613037489747 35.0 33.0 35.0 27.0 36.0 94-95 31.895258165732997 35.0 33.0 35.0 27.0 35.5 96-97 31.735592126131316 35.0 33.0 35.0 26.0 35.0 98-99 31.592791696790965 35.0 33.0 35.0 25.5 35.0 100 31.477548555572387 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21928191054432844 1101 2 0.25775402882776177 1101 3 0.3762156935981231 1101 4 0.22941631841663224 1101 5 0.20466561901316283 1101 6 0.18997760948116849 1101 7 0.13861865396040685 1101 8 0.19171704724887917 1101 9 0.2576423364639666 1101 10-11 0.28443437384603953 1101 12-13 0.223666179714769 1101 14-15 0.3726811082883259 1101 16-17 0.30625715252926966 1101 18-19 0.3185033466385008 1101 20-21 0.3526171581562565 1101 22-23 0.32460032741855827 1101 24-25 0.35634673079774615 1101 26-27 0.41361136236680807 1101 28-29 0.3815617020937694 1101 30-31 0.3890047849932188 1101 32-33 0.3617098679622117 1101 34-35 0.37127264425753026 1101 36-37 0.4100426354421245 1101 38-39 0.34878676113854823 1101 40-41 0.37722505443670684 1101 42-43 0.371983592385007 1101 44-45 0.3587108244942101 1101 46-47 0.3739999680144592 1101 48-49 0.40115201810198187 1101 50-51 0.349213382487207 1101 52-53 0.3721929246907365 1101 54-55 0.3426775019491828 1101 56-57 0.3641221502806147 1101 58-59 0.34763220897480096 1101 60-61 0.31307169951809755 1101 62-63 0.30734041589636263 1101 64-65 0.29354199106526835 1101 66-67 0.32653631527045945 1101 68-69 0.31158913061835136 1101 70-71 0.3314272207670186 1101 72-73 0.35502541412831334 1101 74-75 0.36052689659655357 1101 76-77 0.32611273260613416 1101 78-79 0.33622689980357023 1101 80-81 0.33038127951938634 1101 82-83 0.28982185220459655 1101 84-85 0.351211706436267 1101 86-87 0.3104451710676628 1101 88-89 0.29238479820811847 1101 90-91 0.2997626065875849 1101 92-93 0.2948625872350732 1101 94-95 0.3037375198540566 1101 96-97 0.32271982039054947 1101 98-99 0.33500029445569623 1101 100 0.38114773225341025 1102 1 0.24092610270079007 1102 2 0.2568950480729839 1102 3 0.3908245566733335 1102 4 0.23571579632193362 1102 5 0.2293627442309898 1102 6 0.18264560754953862 1102 7 0.1872690448626244 1102 8 0.18052244566795395 1102 9 0.24126162645528382 1102 10-11 0.2653347961626622 1102 12-13 0.23473338347874062 1102 14-15 0.3155748312199691 1102 16-17 0.33440159427809846 1102 18-19 0.3200938627138612 1102 20-21 0.318288004758422 1102 22-23 0.31565479728805457 1102 24-25 0.2904399095004564 1102 26-27 0.29511745373131504 1102 28-29 0.3162763120234757 1102 30-31 0.2857224440898847 1102 32-33 0.3333127473831752 1102 34-35 0.3427300745908326 1102 36-37 0.36550697325648684 1102 38-39 0.2871020021926185 1102 40-41 0.36549398380865483 1102 42-43 0.3483811923226767 1102 44-45 0.39705755873882964 1102 46-47 0.3117352135161582 1102 48-49 0.2857527682348717 1102 50-51 0.2900418236087319 1102 52-53 0.2891717196163057 1102 54-55 0.29487284121671564 1102 56-57 0.32777036282301 1102 58-59 0.33226550853566295 1102 60-61 0.2904395790645111 1102 62-63 0.25031156449412606 1102 64-65 0.24588824674775367 1102 66-67 0.2600947304897545 1102 68-69 0.33328951980029586 1102 70-71 0.26233203858969745 1102 72-73 0.2996106320301877 1102 74-75 0.2871120150389501 1102 76-77 0.2628619371857681 1102 78-79 0.27002957425559515 1102 80-81 0.23949694525708765 1102 82-83 0.27405984192366617 1102 84-85 0.22359236532998494 1102 86-87 0.24710643955305045 1102 88-89 0.2558673892596417 1102 90-91 0.25974122955423695 1102 92-93 0.24820738553502508 1102 94-95 0.21323198955940725 1102 96-97 0.2809105104472529 1102 98-99 0.317505673864229 1102 100 0.32264402277355586 1103 1 0.2529563968479174 1103 2 0.2327858790986923 1103 3 0.37309783390236007 1103 4 0.24804872160103741 1103 5 0.23978518729736464 1103 6 0.1666429705043342 1103 7 0.18407940281382906 1103 8 0.1712419840843893 1103 9 0.2551452752233416 1103 10-11 0.2252413337114092 1103 12-13 0.24286974991731825 1103 14-15 0.3053367171098884 1103 16-17 0.2934085504913213 1103 18-19 0.2682956813859647 1103 20-21 0.2852160022690029 1103 22-23 0.27384144707897917 1103 24-25 0.2895570151923792 1103 26-27 0.2958709323621491 1103 28-29 0.3061635498234665 1103 30-31 0.32968031007951026 1103 32-33 0.308884100886047 1103 34-35 0.34381125892943487 1103 36-37 0.33067218489320993 1103 38-39 0.37726797080557617 1103 40-41 0.37403526333753945 1103 42-43 0.37772767649306616 1103 44-45 0.35540969066489936 1103 46-47 0.34718095928872117 1103 48-49 0.3318416292770934 1103 50-51 0.2884593574430454 1103 52-53 0.315595164155944 1103 54-55 0.3120491604837099 1103 56-57 0.29805717084502703 1103 58-59 0.32308316868646614 1103 60-61 0.2941247742448496 1103 62-63 0.2779276051770907 1103 64-65 0.2995500784121816 1103 66-67 0.29223439826735387 1103 68-69 0.29177585249994564 1103 70-71 0.2918158227327936 1103 72-73 0.27947778584565697 1103 74-75 0.2786701908646094 1103 76-77 0.28662969100451363 1103 78-79 0.2917411205965905 1103 80-81 0.3309182153248287 1103 82-83 0.2782460211731177 1103 84-85 0.31329071973493683 1103 86-87 0.28770765063115533 1103 88-89 0.273089726810646 1103 90-91 0.2693838277315379 1103 92-93 0.30266911252284245 1103 94-95 0.2652068252368487 1103 96-97 0.3311754479369533 1103 98-99 0.3556650389603675 1103 100 0.3393952317338069 1104 1 0.1821425965427217 1104 2 0.1914836008533527 1104 3 0.2878167566571399 1104 4 0.20010132496978628 1104 5 0.17376410085161353 1104 6 0.1803646793420839 1104 7 0.1723072406120707 1104 8 0.12464350741640828 1104 9 0.18448558069766108 1104 10-11 0.1965649492162811 1104 12-13 0.16522727894327716 1104 14-15 0.2269352209091693 1104 16-17 0.23071007147195388 1104 18-19 0.2197646121294099 1104 20-21 0.25453410831138257 1104 22-23 0.23511134864059358 1104 24-25 0.24083010618894463 1104 26-27 0.27975694231872694 1104 28-29 0.27276190590789895 1104 30-31 0.26679955696288005 1104 32-33 0.2598568906046097 1104 34-35 0.2068254136296872 1104 36-37 0.21348776259996782 1104 38-39 0.24006207258171486 1104 40-41 0.18765507907381362 1104 42-43 0.20995442662767516 1104 44-45 0.19718984152697772 1104 46-47 0.21271181330364186 1104 48-49 0.2043176376721192 1104 50-51 0.22201556535561906 1104 52-53 0.187746585745586 1104 54-55 0.1984175034426272 1104 56-57 0.15189878796692824 1104 58-59 0.21776709809444128 1104 60-61 0.21613983392467162 1104 62-63 0.19322108444064412 1104 64-65 0.22760593994850353 1104 66-67 0.24727436267108516 1104 68-69 0.21344170221304637 1104 70-71 0.22123911380761996 1104 72-73 0.23236547854570944 1104 74-75 0.2651778922346182 1104 76-77 0.20504334202727392 1104 78-79 0.22285523423814624 1104 80-81 0.22694996738574957 1104 82-83 0.18704854812203564 1104 84-85 0.16072486453528256 1104 86-87 0.24104279271777074 1104 88-89 0.2302427009135286 1104 90-91 0.1965213972856361 1104 92-93 0.2500045838025571 1104 94-95 0.22263970993907733 1104 96-97 0.1858892579491247 1104 98-99 0.2346790513720336 1104 100 0.19914274183418357 1105 1 0.12664525526584924 1105 2 0.1590750768051521 1105 3 0.24645569157067726 1105 4 0.13276269044129663 1105 5 0.13557649792943494 1105 6 0.1264279348814341 1105 7 0.13822170418077206 1105 8 0.11226132137695544 1105 9 0.13850977946903953 1105 10-11 0.1568052217178746 1105 12-13 0.15529492507047848 1105 14-15 0.1539912189246877 1105 16-17 0.19004492766993053 1105 18-19 0.17437823095960425 1105 20-21 0.1861370866761476 1105 22-23 0.1596231996416364 1105 24-25 0.18833670119393986 1105 26-27 0.16962884100152564 1105 28-29 0.1457032469206112 1105 30-31 0.1288821065897494 1105 32-33 0.14798410684862517 1105 34-35 0.17539680763899668 1105 36-37 0.1466308504806264 1105 38-39 0.11526883667533383 1105 40-41 0.15753662366240206 1105 42-43 0.1479192865701009 1105 44-45 0.1222375463051435 1105 46-47 0.17528252888206453 1105 48-49 0.15040898774219613 1105 50-51 0.07156832840809813 1105 52-53 0.07523383204065226 1105 54-55 0.10596061642715426 1105 56-57 0.0988640848808302 1105 58-59 0.10536104826675086 1105 60-61 0.12361356210895735 1105 62-63 0.09798552791526305 1105 64-65 0.0565785258829834 1105 66-67 0.08215821090206532 1105 68-69 0.08252530445145112 1105 70-71 0.08341519105866979 1105 72-73 0.14796903671237516 1105 74-75 0.1046187559676639 1105 76-77 0.09795190523010433 1105 78-79 0.09903041980643934 1105 80-81 0.14910097366839636 1105 82-83 0.11979675761109831 1105 84-85 0.04042993751274793 1105 86-87 0.04071397953682521 1105 88-89 0.06638878430484141 1105 90-91 0.052520804856982295 1105 92-93 0.07244759783306876 1105 94-95 0.06905861752941078 1105 96-97 0.09888544179290548 1105 98-99 0.13473229263168918 1105 100 0.1373334726063291 1106 1 0.159301281174276 1106 2 0.14836950994317277 1106 3 0.2658138442585418 1106 4 0.1694662498445112 1106 5 0.13539276191029614 1106 6 0.11670631850039825 1106 7 0.10857016315051737 1106 8 0.09528432562063927 1106 9 0.15058473148191354 1106 10-11 0.1431830745102971 1106 12-13 0.14667127262669055 1106 14-15 0.18352103888270221 1106 16-17 0.20073512932447812 1106 18-19 0.2155593110874321 1106 20-21 0.21453820073795526 1106 22-23 0.1731905123373565 1106 24-25 0.15508227491559268 1106 26-27 0.18091767390815505 1106 28-29 0.19873776687354905 1106 30-31 0.22576303335871728 1106 32-33 0.16665738995138213 1106 34-35 0.19137759691995626 1106 36-37 0.15139289113317034 1106 38-39 0.15692222088400598 1106 40-41 0.1650643631720996 1106 42-43 0.1929673195630386 1106 44-45 0.2442825654470795 1106 46-47 0.2273903571285416 1106 48-49 0.23815762867940293 1106 50-51 0.2204463985183409 1106 52-53 0.18491926764874478 1106 54-55 0.24478781681086303 1106 56-57 0.2106946465931827 1106 58-59 0.22900805478687403 1106 60-61 0.1937320416726962 1106 62-63 0.1905828095534119 1106 64-65 0.19892149198155096 1106 66-67 0.15108556173089482 1106 68-69 0.18376552488942366 1106 70-71 0.18404630204274497 1106 72-73 0.2383195194411698 1106 74-75 0.2352374569589344 1106 76-77 0.2145124534557823 1106 78-79 0.23850624127687325 1106 80-81 0.1923033462565158 1106 82-83 0.2350492006579188 1106 84-85 0.18886217033320207 1106 86-87 0.1980672179288021 1106 88-89 0.16978547485695827 1106 90-91 0.15910003275735107 1106 92-93 0.17133119641652428 1106 94-95 0.16020647102147834 1106 96-97 0.18953479377557514 1106 98-99 0.1971172743570193 1106 100 0.18713770108524486 1107 1 0.09971799248415891 1107 2 0.13530486195787006 1107 3 0.2546570794009604 1107 4 0.14060524953288933 1107 5 0.16407119505680612 1107 6 0.14252482971911462 1107 7 0.08131863673000339 1107 8 0.140963477513516 1107 9 0.17046116056511806 1107 10-11 0.1383955698090773 1107 12-13 0.1479574692155552 1107 14-15 0.20686894346030726 1107 16-17 0.15013039021944508 1107 18-19 0.15213952205711934 1107 20-21 0.16531734853082725 1107 22-23 0.1865743527232624 1107 24-25 0.2085471133886827 1107 26-27 0.22546507775686564 1107 28-29 0.24321969526122444 1107 30-31 0.18473271470543295 1107 32-33 0.18893479358187903 1107 34-35 0.15772398766775098 1107 36-37 0.175335658565686 1107 38-39 0.18402058614564254 1107 40-41 0.22102541631561934 1107 42-43 0.21454274304312548 1107 44-45 0.19166122895637727 1107 46-47 0.20980739266334325 1107 48-49 0.2428657156444558 1107 50-51 0.24153983380961108 1107 52-53 0.19886369848943275 1107 54-55 0.25908503666910576 1107 56-57 0.19655750051385468 1107 58-59 0.19663314432813905 1107 60-61 0.22694834370715 1107 62-63 0.17634572307135699 1107 64-65 0.16658734370655992 1107 66-67 0.18250116588897924 1107 68-69 0.2143068090873541 1107 70-71 0.22495448515778804 1107 72-73 0.2846590058746514 1107 74-75 0.2717471259814772 1107 76-77 0.20406907400533925 1107 78-79 0.2620027426454712 1107 80-81 0.22862919848138574 1107 82-83 0.22593387859966896 1107 84-85 0.21463968152270496 1107 86-87 0.2079556359339918 1107 88-89 0.2091659047652925 1107 90-91 0.19155214861180525 1107 92-93 0.2536336432876922 1107 94-95 0.24331165014064382 1107 96-97 0.1970476225186033 1107 98-99 0.23647983942758088 1107 100 0.24575934654855658 1108 1 0.0047139507625182375 1108 2 0.053315816941776006 1108 3 0.13937217345957365 1108 4 0.05935191436118714 1108 5 0.05101733578589318 1108 6 0.039489342045122555 1108 7 0.03844776545087569 1108 8 0.002198829194504981 1108 9 0.04219916320438699 1108 10-11 0.012779261987276413 1108 12-13 -0.0013399917758718516 1108 14-15 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2215 8 -0.2502635204883177 2215 9 -0.39791806352052106 2215 10-11 -0.4283526647032545 2215 12-13 -0.4117504038838149 2215 14-15 -0.5113238946082532 2215 16-17 -0.5168404626606744 2215 18-19 -0.49235007842138145 2215 20-21 -0.4728798693784029 2215 22-23 -0.45487956496810256 2215 24-25 -0.45990604935011703 2215 26-27 -0.5064264472480033 2215 28-29 -0.44166884196084766 2215 30-31 -0.4606025311376527 2215 32-33 -0.4567598967412181 2215 34-35 -0.5235135091406917 2215 36-37 -0.441152809174028 2215 38-39 -0.44973497810821783 2215 40-41 -0.4945797789647415 2215 42-43 -0.43997224315441485 2215 44-45 -0.4632558602258996 2215 46-47 -0.47150014440258303 2215 48-49 -0.4960496611365173 2215 50-51 -0.4720153287992943 2215 52-53 -0.49027602889700006 2215 54-55 -0.4584410586474945 2215 56-57 -0.42113225165769563 2215 58-59 -0.41376195876341626 2215 60-61 -0.4640514969820799 2215 62-63 -0.477505464833861 2215 64-65 -0.48419147792137807 2215 66-67 -0.4565530860726028 2215 68-69 -0.45976330817376976 2215 70-71 -0.47283768668459913 2215 72-73 -0.3958465774751474 2215 74-75 -0.3920308237420258 2215 76-77 -0.4104476423635788 2215 78-79 -0.4077271481243798 2215 80-81 -0.49061355813067564 2215 82-83 -0.47663772291018347 2215 84-85 -0.438532355574317 2215 86-87 -0.3880239093353737 2215 88-89 -0.40509543224805356 2215 90-91 -0.4341561902287623 2215 92-93 -0.42056753169978833 2215 94-95 -0.4318511612583542 2215 96-97 -0.454275776058676 2215 98-99 -0.4000021050425957 2215 100 -0.3529524609687762 2216 1 -0.3407819052092016 2216 2 -0.40827323258743675 2216 3 -0.3929582677545618 2216 4 -0.2989268462149468 2216 5 -0.35399860943726935 2216 6 -0.30196295911550664 2216 7 -0.45549481987986695 2216 8 -0.3499629749456119 2216 9 -0.5081052038201861 2216 10-11 -0.45124714914022945 2216 12-13 -0.4255684332257488 2216 14-15 -0.6698164143394365 2216 16-17 -0.6062876486101345 2216 18-19 -0.5515481275650274 2216 20-21 -0.5253079961733889 2216 22-23 -0.51343246888365 2216 24-25 -0.5501436452515449 2216 26-27 -0.5994015097051175 2216 28-29 -0.6549043523596723 2216 30-31 -0.6798563657356596 2216 32-33 -0.6803380447773435 2216 34-35 -0.6751846526082019 2216 36-37 -0.74815279664179 2216 38-39 -0.7029417233060542 2216 40-41 -0.7197129197669696 2216 42-43 -0.763110769483653 2216 44-45 -0.8095171625960376 2216 46-47 -0.773273646000888 2216 48-49 -0.7484400852223203 2216 50-51 -0.7794947535187973 2216 52-53 -0.7772217770485241 2216 54-55 -0.7327469919664154 2216 56-57 -0.7552945255942092 2216 58-59 -0.7147344086723919 2216 60-61 -0.753743117142939 2216 62-63 -0.715032859066902 2216 64-65 -0.7571062941198363 2216 66-67 -0.678786274271765 2216 68-69 -0.7023496059580694 2216 70-71 -0.6964848298900037 2216 72-73 -0.7096042846799051 2216 74-75 -0.7018221650932404 2216 76-77 -0.5783573628405847 2216 78-79 -0.6476638795218292 2216 80-81 -0.7373829990966527 2216 82-83 -0.7049486745660758 2216 84-85 -0.801031103885613 2216 86-87 -0.734865288187315 2216 88-89 -0.8055046088266096 2216 90-91 -0.6914744464339293 2216 92-93 -0.7535381184871213 2216 94-95 -0.761982259124931 2216 96-97 -0.7632409010656076 2216 98-99 -0.8014849563304374 2216 100 -0.8221859120993251 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 374.0 3 56.0 4 70.0 5 173.0 6 448.0 7 1166.0 8 2738.0 9 4235.0 10 5627.0 11 7568.0 12 10700.0 13 14386.0 14 15082.0 15 17925.0 16 23569.0 17 33065.0 18 32749.0 19 20461.0 20 17378.0 21 17863.0 22 19583.0 23 21836.0 24 25992.0 25 32515.0 26 41941.0 27 54897.0 28 72512.0 29 95081.0 30 123111.0 31 159031.0 32 204298.0 33 270235.0 34 371894.0 35 554821.0 36 934804.0 37 1702202.0 38 1993507.0 39 601202.0 40 1304.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.45872377283901 18.991134007532068 13.791890521020397 23.758251698608525 2 16.46190137241572 20.160332537612245 37.94734332667368 25.430422763298356 3 18.32680357119306 25.233683954183622 29.82298169868135 26.616530775941964 4 13.426064881443153 17.169657514874974 36.45765432932622 32.94662327435565 5 13.817381225185857 36.346025740094326 34.13410962189241 15.702483412827414 6 31.32368057712977 36.27251881997041 17.860334424492848 14.543466178406977 7 27.894107414220855 31.453430599679024 21.901193901363357 18.75126808473677 8 26.4746667206651 34.07690859695425 20.134362838280072 19.31406184410058 9 25.715939160708086 16.319862559930534 20.78286805697379 37.181330222387594 10-11 24.87412406401525 25.901467534566176 27.881891170453372 21.342517230965207 12-13 25.48214744553372 23.383898788524913 28.183665408476045 22.950288357465325 14-15 22.80483052021033 25.06826677754099 25.799709620987915 26.327193081260763 16-17 22.45442854822932 28.169905436681425 26.14425372272377 23.231412292365487 18-19 22.480043024630454 26.896582089039246 28.491601431205755 22.13177345512455 20-21 23.309161946691862 26.12189657992997 28.366322533476783 22.202618939901384 22-23 23.067412217229595 26.03186960884973 28.339420806168174 22.561297367752502 24-25 22.801710488646147 26.140291348987454 28.334401089844352 22.723597072522047 26-27 22.72712285505378 26.150091098995667 28.320260812298102 22.802525233652453 28-29 22.734386974181778 26.08975732591369 28.47192946217983 22.7039262377247 30-31 22.898602911995486 26.390250504935853 28.14795749599775 22.56318908707091 32-33 22.670540165670815 26.211489235540142 28.37675066641541 22.74121993237363 34-35 22.827151483404855 25.97466707227629 28.381987282446374 22.816194161872478 36-37 23.097420979560564 26.164125489507516 28.113729403982322 22.624724126949598 38-39 22.942805514449827 26.321521083109477 28.053672418745208 22.682000983695488 40-41 22.89425369263499 26.084586947834943 28.23106352856888 22.79009583096119 42-43 22.809179096479443 26.230221578799316 28.08293849706413 22.877660827657106 44-45 22.89457992312681 26.347758735626243 27.896579553308463 22.861081787938488 46-47 23.034144159908486 26.37290464455401 27.644334126634845 22.948617068902664 48-49 23.055743118461844 26.375470009107598 27.574997796871337 22.99378907555922 50-51 23.026364037900823 26.457278577871186 27.4425270608118 23.073830323416193 52-53 23.005384952892403 26.499253068673116 27.393705580699724 23.101656397734754 54-55 23.043734390780322 26.730744149544233 27.35272163776392 22.872799821911517 56-57 22.849637431733903 26.49765442847647 27.421275463091092 23.23143267669853 58-59 22.82361181995134 26.237215397595087 27.58595906182903 23.353213720624538 60-61 23.03841026165365 26.507033838158613 27.417324633637264 23.037231266550474 62-63 22.8603228246531 26.55416748930175 27.509509927973134 23.075999758072015 64-65 22.908502367750764 26.302073081644956 27.54616053225617 23.24326401834811 66-67 22.7660831984864 26.378317932262824 27.542718662018245 23.312880207232528 68-69 23.02366408827296 26.48575813047961 27.36666527673292 23.12391250451451 70-71 23.04518188355043 26.265028460779877 27.39380786950305 23.29598178616664 72-73 22.821120663671476 26.504415020080625 27.420024700372597 23.254439615875302 74-75 22.862031089615282 26.409067504711004 27.332843526793592 23.396057878880125 76-77 22.937261248724166 26.455085135318672 27.176352202564928 23.431301413392234 78-79 22.981059429522784 26.554387121368606 27.276774179323766 23.187779269784844 80-81 22.816557585136348 26.4435917398584 27.310362265783784 23.429488409221467 82-83 22.83583626563664 26.453929743820183 27.297944174842325 23.412289815700856 84-85 22.851928377729283 26.45466376128361 27.130656243062074 23.56275161792503 86-87 22.942049379864137 26.56133702461346 27.09384028619011 23.402773309332286 88-89 22.76768327129553 26.46427962179289 27.179238055766547 23.588799051145035 90-91 22.671729807945365 26.404293314307477 27.20123981593801 23.72273706180915 92-93 22.79486833965613 26.60080291184602 27.03190058861862 23.572428159879227 94-95 22.754675574241563 26.35070238942228 27.161817576384973 23.732804459951183 96-97 22.705916920160586 26.43110688323342 27.137177408487567 23.72579878811843 98-99 22.624780857604055 26.551149145788454 27.093483902979777 23.730586093627718 100 22.757629437357927 26.344324600774836 27.023509286067416 23.874536675799817 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1389.0 1 1129.0 2 5151.0 3 7618.0 4 3662.0 5 1486.0 6 2190.0 7 4942.0 8 8477.5 9 9621.5 10 7011.5 11 4072.5 12 3467.5 13 3733.5 14 4233.0 15 4698.0 16 5135.0 17 5755.0 18 6486.5 19 7296.0 20 8196.0 21 9603.0 22 11696.5 23 14945.5 24 19523.5 25 25312.5 26 33125.5 27 43320.0 28 56207.5 29 70667.0 30 84211.5 31 97662.5 32 111868.5 33 124535.5 34 136878.5 35 150661.0 36 165982.0 37 182358.5 38 198661.0 39 211779.5 40 223469.5 41 236165.0 42 244004.0 43 247565.5 44 251852.5 45 256120.5 46 259485.5 47 258493.0 48 256362.0 49 257376.5 50 258352.5 51 257080.0 52 251154.5 53 248124.0 54 245629.5 55 236028.0 56 223285.0 57 206307.0 58 188717.5 59 175700.5 60 160154.0 61 139357.5 62 118866.0 63 99179.0 64 80706.5 65 66044.5 66 52340.0 67 39979.0 68 30386.0 69 22633.0 70 16641.5 71 12194.0 72 9218.0 73 6938.5 74 5369.0 75 3927.0 76 2558.0 77 1736.5 78 1232.0 79 773.0 80 405.0 81 195.5 82 97.0 83 40.5 84 22.0 85 19.0 86 13.0 87 14.0 88 12.0 89 6.0 90 3.5 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08967016008608122 2 0.0 3 0.0 4 0.0 5 0.007979858251606397 6 1.4654163734168676E-4 7 0.0 8 1.0657573624849945E-4 9 0.0 10-11 0.0 12-13 0.01391479456394471 14-15 3.3304917577656074E-5 16-17 0.0 18-19 1.3321967031062431E-5 20-21 3.996590109318729E-5 22-23 0.0 24-25 7.993180218637458E-5 26-27 5.994885163978094E-5 28-29 1.9316852195040522E-4 30-31 0.0 32-33 9.991475273296823E-5 34-35 3.3304917577656074E-5 36-37 3.3304917577656074E-5 38-39 1.4654163734168676E-4 40-41 5.994885163978094E-5 42-43 2.3313442304359253E-4 44-45 1.4654163734168676E-4 46-47 7.327081867084338E-5 48-49 3.796760603852793E-4 50-51 2.4645639007465496E-4 52-53 2.597783571057174E-4 54-55 2.3979540655912377E-4 56-57 2.597783571057174E-4 58-59 7.993180218637458E-5 60-61 8.65927857019058E-5 62-63 4.396249120250602E-4 64-65 1.7984655491934282E-4 66-67 6.46115401006528E-4 68-69 6.128104834288718E-4 70-71 2.198124560125301E-4 72-73 4.5960786257165387E-4 74-75 4.529468790561226E-4 76-77 3.39710159292092E-4 78-79 0.001392145554746024 80-81 0.0011590111317024315 82-83 9.325376921743701E-4 84-85 0.0013655016206838993 86-87 7.993180218637459E-4 88-89 0.0011789940822490251 90-91 0.0016452629283362102 92-93 0.0012722478514664622 94-95 0.0017118727634915223 96-97 0.0018317704667710842 98-99 0.0019916340711438334 100 6.128104834288718E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7506399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.966120261476156 #Duplication Level Percentage of deduplicated Percentage of total 1 73.6459161637862 39.0073845229769 2 14.825293334927265 15.704765393788367 3 4.719253359098679 7.498816228871877 4 2.137131941132257 4.527823496346125 5 1.1535507197382968 3.05495530746855 6 0.7572820401433135 2.406617496605205 7 0.5100506866014101 1.8910784204185138 8 0.36056316648909165 1.52781056304959 9 0.26732797249566953 1.2743392986416 >10 1.4788416694493907 14.16830106378655 >50 0.10035794331490898 3.6621668202250186 >100 0.04291883938216886 3.844574417773379 >500 0.0011089781204945692 0.37696920927514355 >1k 2.771899120926077E-4 0.2486007814641614 >5k 5.039816353829213E-5 0.20052496674241543 >10k+ 7.559724530743818E-5 0.6052720125666798 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17840 0.23766389183415376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15828 0.21086009416765614 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11681 0.15561389688984026 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9498 0.12653204286103098 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.3321967031062431E-5 0.0 1.3321967031062431E-5 0.0 3 0.0 1.3321967031062431E-5 0.0 2.6643934062124863E-5 0.0 4 0.0 1.3321967031062431E-5 0.0 2.6643934062124863E-5 0.0 5 0.0 1.3321967031062431E-5 0.0 2.6643934062124863E-5 0.0 6 0.0 1.3321967031062431E-5 0.0 3.996590109318729E-5 0.0 7 0.0 1.3321967031062431E-5 0.0 3.996590109318729E-5 0.0 8 0.0 1.3321967031062431E-5 0.0 3.996590109318729E-5 0.0 9 0.0 1.3321967031062431E-5 0.0 3.996590109318729E-5 0.0 10-11 0.0 1.3321967031062431E-5 0.0 4.662688460871851E-5 0.0 12-13 0.0 1.9982950546593645E-5 0.0 7.993180218637458E-5 0.0 14-15 1.3321967031062431E-5 4.662688460871851E-5 0.0 7.993180218637458E-5 0.0 16-17 1.3321967031062431E-5 6.660983515531216E-5 0.0 8.65927857019058E-5 0.0 18-19 1.3321967031062431E-5 1.3321967031062432E-4 0.0 1.1989770327956189E-4 0.0 20-21 2.6643934062124863E-5 1.3321967031062432E-4 0.0 1.7984655491934284E-4 0.0 22-23 2.6643934062124863E-5 1.4654163734168673E-4 0.0 4.4628589554059143E-4 0.0 24-25 2.6643934062124863E-5 1.5986360437274916E-4 0.0 9.258767086588389E-4 0.0 26-27 2.6643934062124863E-5 1.5986360437274916E-4 0.0 0.0014454334228702738 0.0 28-29 2.6643934062124863E-5 1.5986360437274916E-4 0.0 0.003157306186361796 0.0 30-31 2.6643934062124863E-5 1.6652458788828038E-4 0.0 0.007546894323096867 0.0 32-33 2.6643934062124863E-5 1.731855714038116E-4 0.0 0.017358523041474345 0.0 34-35 2.6643934062124863E-5 1.731855714038116E-4 0.0 0.03211926251189152 0.0 36-37 2.6643934062124863E-5 1.8650753843487403E-4 0.0 0.0554593487503129 0.0 38-39 2.6643934062124863E-5 1.9982950546593647E-4 0.0 0.09054940991013134 0.0 40-41 2.6643934062124863E-5 1.9982950546593647E-4 0.0 0.13397236144787933 0.0 42-43 2.6643934062124863E-5 1.9982950546593647E-4 0.0 0.17930035427106927 0.0 44-45 2.6643934062124863E-5 1.9982950546593647E-4 0.0 0.23072980799448578 0.0 46-47 2.6643934062124863E-5 2.198124560125301E-4 0.0 0.2937959999195353 0.0 48-49 2.6643934062124863E-5 2.3979540655912375E-4 0.0 0.3637363268326131 0.0 50-51 2.6643934062124863E-5 2.79761307652311E-4 0.0 0.43989135136568147 0.0 52-53 2.6643934062124863E-5 3.064052417144359E-4 0.0 0.5164793398272594 0.0 54-55 2.6643934062124863E-5 3.064052417144359E-4 0.0 0.6023061124248791 0.0 56-57 3.996590109318729E-5 3.064052417144359E-4 0.0 0.7017412743447291 0.0 58-59 3.996590109318729E-5 3.064052417144359E-4 0.0 0.8108948112137391 0.0 60-61 3.996590109318729E-5 3.064052417144359E-4 0.0 0.927282176180616 0.0 62-63 3.996590109318729E-5 3.064052417144359E-4 0.0 1.0450417037516924 0.0 64-65 3.996590109318729E-5 3.1972720874549833E-4 0.0 1.1747643577166627 0.0 66-67 3.996590109318729E-5 3.3304917577656076E-4 0.0 1.3232510022448847 0.0 68-69 3.996590109318729E-5 3.39710159292092E-4 0.0 1.481649190244217 0.0 70-71 3.996590109318729E-5 3.663540933542169E-4 0.0 1.6497391092586473 0.0 72-73 3.996590109318729E-5 3.7301507686974807E-4 0.0 1.8318104326721774 0.0 74-75 3.996590109318729E-5 3.7301507686974807E-4 0.0 2.019830014365077 0.0 76-77 3.996590109318729E-5 3.7301507686974807E-4 0.0 2.2214912902977844 0.0 78-79 3.996590109318729E-5 3.7301507686974807E-4 0.0 2.4416301345025757 0.0 80-81 3.996590109318729E-5 3.7301507686974807E-4 0.0 2.680479681402494 0.0 82-83 3.996590109318729E-5 3.7301507686974807E-4 0.0 2.9317186576413006 0.0 84-85 3.996590109318729E-5 3.7301507686974807E-4 0.0 3.181845249633013 0.0 86-87 3.996590109318729E-5 3.7967606038527926E-4 0.0 3.4481047436993424 0.0 88 3.996590109318729E-5 3.863370439008105E-4 0.0 3.66493974008043 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11045 0.0 48.730247 1 TATAACG 1030 0.0 47.45826 2 TAACGCA 1315 0.0 38.60235 4 GTATCAA 18555 0.0 37.602654 1 ATAACGC 1440 0.0 36.55706 3 TACCTGG 7010 0.0 33.659016 2 GTACCTG 7585 0.0 32.006077 1 ATCAACG 22320 0.0 30.76605 3 TCAACGC 22410 0.0 30.705412 4 TATCACG 1410 0.0 30.33458 2 CAACGCA 23545 0.0 29.325054 5 TATCAAC 24160 0.0 28.520212 2 AACGCAG 24815 0.0 28.210194 6 GTATAAC 2340 0.0 26.941843 1 ACGCAGA 28390 0.0 24.707352 7 ACCTGGG 9635 0.0 24.04977 3 CGCAGAG 29550 0.0 23.673813 8 GTACATG 24435 0.0 23.470896 1 TACATGG 25240 0.0 21.992586 2 TCACGCA 2125 0.0 21.233812 4 >>END_MODULE