FastQCFastQC Report
Wed 25 May 2016
SRR2049433_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049433_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9370424
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.035.4482881
GTATCAA138850.031.8684791
TACCTGG82450.030.385172
TATAACG10150.030.1004122
GTACCTG90400.028.7136381
TCAACGC145950.028.5644664
ATCAACG148550.027.939153
TAACGCA12700.027.3863774
CAACGCA159100.026.441295
AACGCAG167500.025.2549136
TATCAAC172750.024.5422082
ATAACGC14550.023.2591923
GTACATG295050.023.236971
TACATGG305100.021.5372412
ACCTGGG114300.021.4659163
ACGCAGA197300.021.3452597
ACATGGG305800.020.6271933
GTATAAC27600.020.61551
TATCACG15700.020.3579672
CGCAGAG207050.020.225748
Produced by FastQC (version 0.11.5)