##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049433_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9370424
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.520084149874116	31.0	31.0	34.0	27.0	34.0
2	30.63987339313568	31.0	31.0	34.0	27.0	34.0
3	30.724764749172504	31.0	31.0	34.0	27.0	34.0
4	34.29101607355228	37.0	35.0	37.0	30.0	37.0
5	34.2948878300491	37.0	35.0	37.0	30.0	37.0
6	34.3251577516663	37.0	35.0	37.0	30.0	37.0
7	34.195400229488015	37.0	35.0	37.0	30.0	37.0
8	34.185609957457636	37.0	35.0	37.0	30.0	37.0
9	35.72164013069206	39.0	37.0	39.0	30.0	39.0
10-11	35.66734285449623	39.0	37.0	39.0	30.0	39.0
12-13	35.62481377576938	39.0	37.0	39.0	30.0	39.0
14-15	36.81431656667831	40.0	37.0	41.0	30.0	41.0
16-17	36.785945545260276	40.0	37.0	41.0	30.0	41.0
18-19	36.7768306962417	40.0	37.0	41.0	30.0	41.0
20-21	36.80782123626423	40.0	37.0	41.0	30.0	41.0
22-23	36.74834196403492	40.0	37.0	41.0	30.0	41.0
24-25	36.721225048087476	40.0	37.0	41.0	30.0	41.0
26-27	36.68938262558877	40.0	37.0	41.0	30.0	41.0
28-29	36.63718183937034	40.0	37.0	41.0	30.0	41.0
30-31	36.574202992308564	40.0	37.0	41.0	30.0	41.0
32-33	36.521210566352174	40.0	37.0	41.0	30.0	41.0
34-35	36.43961308474408	40.0	37.0	41.0	30.0	41.0
36-37	36.34417049858149	40.0	36.0	41.0	30.0	41.0
38-39	36.23951370823775	40.0	36.0	41.0	29.0	41.0
40-41	36.11731833052592	40.0	36.0	41.0	29.0	41.0
42-43	35.99875891421776	40.0	36.0	41.0	28.0	41.0
44-45	35.84418373170733	40.0	35.0	41.0	28.0	41.0
46-47	35.70277732363018	39.5	35.0	41.0	27.0	41.0
48-49	35.572874557223884	39.0	35.0	41.0	27.0	41.0
50-51	35.42148605015099	39.0	35.0	41.0	27.0	41.0
52-53	35.2679886737249	39.0	35.0	41.0	26.5	41.0
54-55	35.092113868059755	39.0	35.0	41.0	26.0	41.0
56-57	34.905762695476746	39.0	34.5	40.0	26.0	41.0
58-59	34.68029040094664	38.0	34.0	40.0	26.0	41.0
60-61	34.438418955214836	38.0	34.0	40.0	25.0	41.0
62-63	34.16064150352214	37.0	34.0	40.0	25.0	41.0
64-65	33.890715724283126	37.0	34.0	40.0	24.0	41.0
66-67	33.6204263008803	36.5	33.0	39.5	23.5	41.0
68-69	33.31022689048009	36.0	33.0	39.0	23.0	41.0
70-71	32.99272215430166	35.5	33.0	39.0	22.5	41.0
72-73	32.647783280671185	35.0	33.0	38.5	22.0	40.0
74-75	32.3342345020887	35.0	33.0	37.5	21.5	40.0
76-77	32.02335790781719	35.0	32.0	37.0	20.0	39.0
78-79	31.71290402654138	35.0	32.0	36.5	20.0	39.0
80-81	31.40796526389841	35.0	32.0	36.0	18.5	38.5
82-83	31.14018143682719	35.0	32.0	36.0	18.0	37.0
84-85	30.888503177657704	35.0	32.0	35.0	17.5	37.0
86-87	30.6356729428679	35.0	31.5	35.0	13.5	36.5
88-89	30.434133396738503	35.0	31.0	35.0	8.5	36.0
90-91	30.25390387884262	35.0	31.0	35.0	4.5	36.0
92-93	30.05070090745093	35.0	31.0	35.0	2.0	36.0
94-95	29.853401884482494	34.5	31.0	35.0	2.0	35.0
96-97	29.640981827503218	34.0	31.0	35.0	2.0	35.0
98-99	29.448452866166996	34.0	31.0	35.0	2.0	35.0
100	29.3098172505321	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2944941238254657
1101	2	0.292302778673843
1101	3	0.26226647045799467
1101	4	0.2825228096051191
1101	5	0.3088945586639795
1101	6	0.3348741034352969
1101	7	0.306520136122046
1101	8	0.35075705155211523
1101	9	0.4181943149644738
1101	10-11	0.47283432978243667
1101	12-13	0.5065638325680624
1101	14-15	0.6024319877211894
1101	16-17	0.5463127429084054
1101	18-19	0.5901963041340395
1101	20-21	0.5988698251275579
1101	22-23	0.5899767354409207
1101	24-25	0.5968396458211842
1101	26-27	0.5336116393276953
1101	28-29	0.5534804252331398
1101	30-31	0.5301298553697933
1101	32-33	0.5383138100948557
1101	34-35	0.5567665197370317
1101	36-37	0.5331873431975396
1101	38-39	0.5532412570220444
1101	40-41	0.608297068585351
1101	42-43	0.5436058748020258
1101	44-45	0.5381835931724055
1101	46-47	0.48558516938771135
1101	48-49	0.5260509820085844
1101	50-51	0.5227941801007887
1101	52-53	0.500601139385715
1101	54-55	0.46813704842099924
1101	56-57	0.4566447788762673
1101	58-59	0.48380947365573235
1101	60-61	0.5129478450681404
1101	62-63	0.49081896263516
1101	64-65	0.49700503246832994
1101	66-67	0.4937224515190479
1101	68-69	0.4492752999550973
1101	70-71	0.4984818299672824
1101	72-73	0.5294339810441357
1101	74-75	0.509371414864674
1101	76-77	0.49820990332134585
1101	78-79	0.47930461952076797
1101	80-81	0.535317561750638
1101	82-83	0.43903486179435447
1101	84-85	0.4271621750384469
1101	86-87	0.422490819714735
1101	88-89	0.3952693310229627
1101	90-91	0.4012529619648646
1101	92-93	0.3844427285404102
1101	94-95	0.3848643608170157
1101	96-97	0.4296917895309136
1101	98-99	0.45888237548830446
1101	100	0.49265611443411217
1102	1	0.2280583065131374
1102	2	0.29664421714296907
1102	3	0.33024810111372105
1102	4	0.3191484943988385
1102	5	0.3170218954842454
1102	6	0.3586939582244071
1102	7	0.4302905991757484
1102	8	0.41650009473654137
1102	9	0.4504622777319085
1102	10-11	0.48415981058932545
1102	12-13	0.5426805988236723
1102	14-15	0.649940565320172
1102	16-17	0.5601484533953212
1102	18-19	0.5805809525655263
1102	20-21	0.6257881832219425
1102	22-23	0.5694507780364688
1102	24-25	0.6016747233665782
1102	26-27	0.5357979163019522
1102	28-29	0.5901236558752672
1102	30-31	0.5296905894892348
1102	32-33	0.5259034731527663
1102	34-35	0.6034185466557602
1102	36-37	0.6012174085423965
1102	38-39	0.5972366858523017
1102	40-41	0.6390160536747302
1102	42-43	0.5988616358663137
1102	44-45	0.572539772965662
1102	46-47	0.50431262569073
1102	48-49	0.50331598048583
1102	50-51	0.52699247474186
1102	52-53	0.4804098412119373
1102	54-55	0.43945508870946526
1102	56-57	0.4143802554508582
1102	58-59	0.47028231592130965
1102	60-61	0.47015253103981536
1102	62-63	0.42931115332401504
1102	64-65	0.455885080160904
1102	66-67	0.49831791749624443
1102	68-69	0.4484492422151263
1102	70-71	0.4412760584251174
1102	72-73	0.42271659658597827
1102	74-75	0.4090627892435492
1102	76-77	0.4052580516941333
1102	78-79	0.38229330520837124
1102	80-81	0.3713160891846101
1102	82-83	0.36203716668654096
1102	84-85	0.3885048238670237
1102	86-87	0.37130818850728886
1102	88-89	0.31802742845925636
1102	90-91	0.3683289835645738
1102	92-93	0.335221781136088
1102	94-95	0.365069955701518
1102	96-97	0.4221498115906357
1102	98-99	0.4416693186895557
1102	100	0.39690408195728466
1103	1	0.23106435894823818
1103	2	0.27321764237367674
1103	3	0.2509637181644173
1103	4	0.33257762054269335
1103	5	0.29376511213628476
1103	6	0.2808653423554688
1103	7	0.27348613572070235
1103	8	0.2948256802438536
1103	9	0.3895723356316694
1103	10-11	0.36931317278850173
1103	12-13	0.38665102663821216
1103	14-15	0.4591992065992585
1103	16-17	0.45946530386364515
1103	18-19	0.4234099227536774
1103	20-21	0.4297043483968537
1103	22-23	0.4333724852334342
1103	24-25	0.4259958650341744
1103	26-27	0.4801366454632543
1103	28-29	0.42639783125429886
1103	30-31	0.44656309880824807
1103	32-33	0.48134172318885504
1103	34-35	0.4879470168601401
1103	36-37	0.4749815452225903
1103	38-39	0.46345496444113365
1103	40-41	0.49646936469446956
1103	42-43	0.42932203119814005
1103	44-45	0.37315107788946733
1103	46-47	0.38005767057461526
1103	48-49	0.34256724943897865
1103	50-51	0.30701800506378163
1103	52-53	0.3005144655265255
1103	54-55	0.24939283489397468
1103	56-57	0.19933027848506413
1103	58-59	0.24408205581095643
1103	60-61	0.2881274220331349
1103	62-63	0.3392207528880604
1103	64-65	0.38677875608107826
1103	66-67	0.33285936559124707
1103	68-69	0.3186067519519966
1103	70-71	0.28980742120032943
1103	72-73	0.2991607374378802
1103	74-75	0.3112700058900586
1103	76-77	0.29632591176498124
1103	78-79	0.2553426536547221
1103	80-81	0.2577755949196785
1103	82-83	0.24175624197557255
1103	84-85	0.27777096870138607
1103	86-87	0.253621919170822
1103	88-89	0.2325813198580029
1103	90-91	0.23501673164845016
1103	92-93	0.2580578017929689
1103	94-95	0.2777164356127244
1103	96-97	0.24975668165356169
1103	98-99	0.2689337603052735
1103	100	0.19781021085074002
1104	1	0.07717070777892587
1104	2	0.1260634875486275
1104	3	0.13610128511223252
1104	4	0.15875390336527317
1104	5	0.20737166084088443
1104	6	0.2074566761144041
1104	7	0.1314588348670469
1104	8	0.1643792076703292
1104	9	0.22956021724002795
1104	10-11	0.2359975264731773
1104	12-13	0.25532610060482597
1104	14-15	0.33682170278905943
1104	16-17	0.3002514931399247
1104	18-19	0.3023621936593912
1104	20-21	0.24605299744146691
1104	22-23	0.34584334706454456
1104	24-25	0.3873141736832437
1104	26-27	0.3160568258337051
1104	28-29	0.28074056562220306
1104	30-31	0.3073191352986626
1104	32-33	0.3377160223871698
1104	34-35	0.31667898362605484
1104	36-37	0.29439936389883314
1104	38-39	0.3706145685648323
1104	40-41	0.3613892513861856
1104	42-43	0.3326400468314006
1104	44-45	0.30711160560962014
1104	46-47	0.2615151471515844
1104	48-49	0.21499316450018569
1104	50-51	0.25676970929547593
1104	52-53	0.2366693953187422
1104	54-55	0.2607131310123094
1104	56-57	0.22666448556238095
1104	58-59	0.27423261688320366
1104	60-61	0.20821581999899763
1104	62-63	0.23265701830204222
1104	64-65	0.25555286408989275
1104	66-67	0.27357606371401744
1104	68-69	0.24018456919630893
1104	70-71	0.2237993745352327
1104	72-73	0.2653398443495334
1104	74-75	0.2657399250322783
1104	76-77	0.25110593179878293
1104	78-79	0.27255498604072415
1104	80-81	0.26574085994982966
1104	82-83	0.2710733414372086
1104	84-85	0.23890655746097877
1104	86-87	0.2742255025818814
1104	88-89	0.27023977611544936
1104	90-91	0.26887998076956876
1104	92-93	0.22014870696655464
1104	94-95	0.2701438038202433
1104	96-97	0.2729356964177114
1104	98-99	0.32673960219208453
1104	100	0.2936186341936242
1105	1	0.17458403478703488
1105	2	0.15536598278221447
1105	3	0.17578769771450453
1105	4	0.21384598634997332
1105	5	0.2562667044669098
1105	6	0.24437707126976704
1105	7	0.25073708899160607
1105	8	0.27721537411706265
1105	9	0.32229094969851246
1105	10-11	0.31701455433579895
1105	12-13	0.35967785569096833
1105	14-15	0.36103166003217524
1105	16-17	0.3583188781794959
1105	18-19	0.3475386053250986
1105	20-21	0.3567401651986444
1105	22-23	0.33652660254549005
1105	24-25	0.32716410221124903
1105	26-27	0.3329708106925082
1105	28-29	0.3506556022532621
1105	30-31	0.32417623283567565
1105	32-33	0.3738573961081073
1105	34-35	0.371461495122297
1105	36-37	0.33640706898971473
1105	38-39	0.33195929957354764
1105	40-41	0.34834411226179185
1105	42-43	0.3606322852836996
1105	44-45	0.34942186246824036
1105	46-47	0.2992537612638202
1105	48-49	0.2773693716736645
1105	50-51	0.34611476574286826
1105	52-53	0.31874584933220973
1105	54-55	0.3167889210009349
1105	56-57	0.31291900877730683
1105	58-59	0.30685355554747673
1105	60-61	0.2842204940038613
1105	62-63	0.3396919256485589
1105	64-65	0.35873980698656993
1105	66-67	0.3395719900074141
1105	68-69	0.34560619880939925
1105	70-71	0.33727815864742183
1105	72-73	0.3546238552637959
1105	74-75	0.3630268326189565
1105	76-77	0.3873399694423725
1105	78-79	0.3306660129650325
1105	80-81	0.3322499425223455
1105	82-83	0.2930872732755887
1105	84-85	0.2814311362152182
1105	86-87	0.28498213283442553
1105	88-89	0.2513134515517663
1105	90-91	0.30467376359652576
1105	92-93	0.3015488058613478
1105	94-95	0.29602189470471885
1105	96-97	0.3038442614746586
1105	98-99	0.29029180722093173
1105	100	0.2547145891871878
1106	1	0.1393686788558952
1106	2	0.16062486067758286
1106	3	0.17959432537440279
1106	4	0.22171075335314328
1106	5	0.18851134843265527
1106	6	0.2370401664213304
1106	7	0.22522802273289955
1106	8	0.19093775815141356
1106	9	0.27403790401526607
1106	10-11	0.2281516825412453
1106	12-13	0.27528036259896993
1106	14-15	0.3456651519381424
1106	16-17	0.3074234358291861
1106	18-19	0.3063682990902379
1106	20-21	0.29408752215245215
1106	22-23	0.2983983755628401
1106	24-25	0.31150967849589506
1106	26-27	0.3021616934925575
1106	28-29	0.2956289776403409
1106	30-31	0.3334960579592874
1106	32-33	0.3270557363069031
1106	34-35	0.3178755227393424
1106	36-37	0.32310205393025626
1106	38-39	0.3711290774913252
1106	40-41	0.4043994589852886
1106	42-43	0.3802444771960438
1106	44-45	0.2927536942191651
1106	46-47	0.2713575606076546
1106	48-49	0.27870838150071364
1106	50-51	0.2919253988468853
1106	52-53	0.33400334274568877
1106	54-55	0.3277475602235498
1106	56-57	0.25804225344482745
1106	58-59	0.3249308655421217
1106	60-61	0.3158422904497371
1106	62-63	0.282659151893931
1106	64-65	0.2730085584265183
1106	66-67	0.3157827292803148
1106	68-69	0.31122987984903006
1106	70-71	0.30361017479375363
1106	72-73	0.3055039591317765
1106	74-75	0.31621349899177886
1106	76-77	0.2851266635958112
1106	78-79	0.2527668401462009
1106	80-81	0.30277393631638816
1106	82-83	0.3281919944953273
1106	84-85	0.31898151513057726
1106	86-87	0.32839433176268784
1106	88-89	0.3043204511970252
1106	90-91	0.2935535913768881
1106	92-93	0.29889107576667584
1106	94-95	0.3017348336307215
1106	96-97	0.34322297535112867
1106	98-99	0.3400036894116809
1106	100	0.3398530277726515
1107	1	0.1439503151443553
1107	2	0.15043150706463493
1107	3	0.14996363134127577
1107	4	0.1448893992272886
1107	5	0.17791482095719147
1107	6	0.22082504355523014
1107	7	0.17270875347056602
1107	8	0.21597797124929485
1107	9	0.2203492741190871
1107	10-11	0.25282082769545866
1107	12-13	0.2254399660131483
1107	14-15	0.241661459434674
1107	16-17	0.2250403656849329
1107	18-19	0.23247993114657817
1107	20-21	0.23722957373506404
1107	22-23	0.2457411427225722
1107	24-25	0.260126131902787
1107	26-27	0.2862537023517078
1107	28-29	0.2822270533511073
1107	30-31	0.24733938772993724
1107	32-33	0.2328392165726143
1107	34-35	0.2603514969342271
1107	36-37	0.25510276319047875
1107	38-39	0.2943332094560347
1107	40-41	0.330092224262863
1107	42-43	0.3391669007544067
1107	44-45	0.28164747472909824
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2216	2	-0.667362043475471
2216	3	-0.6932258952463997
2216	4	-0.7433587553177006
2216	5	-0.7386665846945917
2216	6	-1.1595728423364733
2216	7	-1.0576336679647866
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2216	9	-1.2569531822487932
2216	10-11	-0.9517070206790592
2216	12-13	-0.9526911518879828
2216	14-15	-1.1186842330786604
2216	16-17	-1.1845511902754495
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2216	20-21	-1.0581554979247727
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2216	24-25	-1.157781276938259
2216	26-27	-1.1751155015140213
2216	28-29	-1.3155665864573365
2216	30-31	-1.2738080068446394
2216	32-33	-1.283384318370885
2216	34-35	-1.5217000741224709
2216	36-37	-1.2477201248912309
2216	38-39	-1.1669064014804675
2216	40-41	-1.329547511683991
2216	42-43	-1.2279518580533875
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2216	50-51	-1.2527739614443334
2216	52-53	-1.452858275135405
2216	54-55	-1.2223435038767292
2216	56-57	-1.181445794464814
2216	58-59	-1.5181365363345378
2216	60-61	-1.66875299886118
2216	62-63	-1.4719051893121389
2216	64-65	-1.445529206350578
2216	66-67	-1.3680440055444691
2216	68-69	-1.438802742669715
2216	70-71	-1.3212810207092147
2216	72-73	-1.4621199345774976
2216	74-75	-1.6607606934888892
2216	76-77	-1.8435801798488356
2216	78-79	-1.5919650371261085
2216	80-81	-1.5472477812293803
2216	82-83	-1.5607143377862656
2216	84-85	-1.4533097875881253
2216	86-87	-1.4635063584325039
2216	88-89	-1.4895121450397752
2216	90-91	-1.546797444441399
2216	92-93	-1.5315397387178855
2216	94-95	-1.4490063450028607
2216	96-97	-1.547683253740047
2216	98-99	-1.475552811126697
2216	100	-1.4022379853367362
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	227769.0
3	37166.0
4	23434.0
5	18748.0
6	16711.0
7	16811.0
8	18191.0
9	20272.0
10	22662.0
11	24575.0
12	26693.0
13	28408.0
14	30078.0
15	31515.0
16	33029.0
17	35030.0
18	33969.0
19	32031.0
20	31841.0
21	34119.0
22	38497.0
23	45112.0
24	53801.0
25	65258.0
26	78870.0
27	92940.0
28	112391.0
29	138855.0
30	165449.0
31	200378.0
32	254412.0
33	335316.0
34	462994.0
35	681584.0
36	1143265.0
37	1951179.0
38	2170492.0
39	635518.0
40	1061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.264255221914	18.77902991242176	13.364800423689903	23.591914441974343
2	16.307666264166105	19.749350272725884	38.692205119239325	25.250778343868685
3	18.522234212540305	25.124590790368323	29.472902770617388	26.880272226473984
4	13.254096379048013	16.798561631005413	37.2187797025172	32.72856228742938
5	14.093879429556807	36.75995805740551	34.044704469934665	15.101458043103017
6	31.57688122492087	36.43313468376615	17.94148741153761	14.048496679775369
7	28.270394218159282	31.615918933995534	21.718686054750634	18.39500079309455
8	26.407257978439535	34.790521934037784	19.94897000747145	18.853250080051232
9	25.683671414197295	17.113311106156242	20.44862579412064	36.754391685525825
10-11	24.781734652739953	26.413535968538838	27.88642612888775	20.91830324983346
12-13	25.17634968376285	23.80504049590323	27.939905014643596	23.078704805690325
14-15	22.670272185736533	25.759411119898985	25.049359748190035	26.520956946174444
16-17	22.753689697441796	28.904412010748548	25.422826168922974	22.919072122886682
18-19	22.82097032714143	28.161139204149144	26.956243348457214	22.06164712025221
20-21	23.63152067726905	27.327996294083473	26.697456798774443	22.34302622987303
22-23	23.418443147059286	27.193105298151014	26.65926725193806	22.72918430285164
24-25	23.114139520610543	27.304123443118367	26.66006310916017	22.921673927110923
26-27	23.05330258827477	27.334930355867705	26.620171249527914	22.991595806329613
28-29	23.011975322800996	27.340998665637233	26.712124908266894	22.934901103294877
30-31	23.101013660472976	27.605769281571163	26.509746460268214	22.783470597687653
32-33	22.942435016314633	27.425826325210352	26.697378237976967	22.934360420498052
34-35	23.09332151006277	27.143857239558063	26.764092664669604	22.998728585709568
36-37	23.286338514472178	27.291745894753223	26.556372275643405	22.865543315131198
38-39	23.092700159449116	27.47003631101907	26.557404136796197	22.879859392735618
40-41	23.116546856118525	27.234762159886948	26.718510952499862	22.930180031494665
42-43	23.001311322414114	27.35051287371515	26.664123217771312	22.984052586099427
44-45	23.01825043370498	27.44138745032173	26.55926314050707	22.981098975466214
46-47	23.155706907858782	27.316728817698653	26.541909891108318	22.985654383334246
48-49	23.081906158257606	27.318654265557036	26.573934443307788	23.025505132877573
50-51	23.022302998787282	27.348444143880478	26.539024110651937	23.090228746680292
52-53	23.056131910007196	27.268836294955268	26.53761580686335	23.137415988174183
54-55	23.080612426239185	27.45953795323176	26.538299588054816	22.92155003247424
56-57	22.915818522883818	27.315061603258066	26.58915014978922	23.179969724068897
58-59	22.952328131898167	27.051724885951174	26.691201077019457	23.304745905131206
60-61	23.100558875365056	27.284185921784132	26.535957339224943	23.07929786362587
62-63	22.970122138368477	27.39847255124865	26.55970088676094	23.071704423621934
64-65	23.077282719406917	27.137356513117993	26.56796691985443	23.217393847620663
66-67	22.899519077208883	27.194624167497643	26.603321016963715	23.30253573832976
68-69	23.115834325539247	27.25666049906846	26.535329023664044	23.092176151728253
70-71	23.11323929367192	27.035955218215957	26.5974903554756	23.25331513263653
72-73	22.923122673595962	27.21395850963267	26.691080655504766	23.171838161266606
74-75	22.949299321285793	27.117698962915554	26.657877305852946	23.275124409945708
76-77	23.038238868423395	27.17270858277766	26.478382582511685	23.31066996628726
78-79	23.05333099513001	27.206819029146818	26.627670757775334	23.112179217947837
80-81	22.952531807200405	27.111547181621248	26.688849476739406	23.24707153443894
82-83	22.978106183366958	27.07340390763705	26.73854028556614	23.209949623429853
84-85	23.007124279573603	27.064225250940133	26.580690024327524	23.347960445158737
86-87	23.097309332798215	27.163234308355072	26.58046277597271	23.158993582874
88-89	22.970530562588067	27.092887438731424	26.63347383883363	23.30310815984688
90-91	22.895605124000216	27.03106617792253	26.663723556572076	23.409605141505175
92-93	23.003402725916356	27.173041436707923	26.560650032611992	23.26290580476373
94-95	23.031339549518194	26.941228885032814	26.67252762876318	23.35490393668581
96-97	22.99288216840757	26.994006233158906	26.618279158471253	23.394832439962272
98-99	22.939002152232394	27.087844721651656	26.599648914822783	23.37350421129317
100	23.073276403968677	26.883500423800538	26.591098331756307	23.45212484047448
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3945.0
1	3095.5
2	3283.5
3	4075.0
4	3441.5
5	2876.5
6	2539.5
7	2435.0
8	2544.5
9	2570.0
10	2333.5
11	2090.5
12	2109.5
13	2194.0
14	2410.0
15	2657.0
16	2896.5
17	3232.5
18	3787.5
19	4655.0
20	6078.5
21	8407.0
22	11745.5
23	16849.5
24	24088.0
25	33615.0
26	45845.0
27	60799.5
28	78337.5
29	97947.0
30	116117.5
31	133379.5
32	151231.0
33	166835.0
34	181640.0
35	197948.5
36	216413.0
37	235050.5
38	253663.0
39	269082.0
40	282421.0
41	296833.0
42	304841.0
43	308243.0
44	312366.5
45	317037.5
46	321676.0
47	318921.5
48	315044.0
49	316500.5
50	316754.5
51	316135.5
52	309457.0
53	304477.5
54	302642.5
55	291585.5
56	274707.5
57	253481.5
58	231737.5
59	214324.5
60	195131.5
61	170719.0
62	146507.0
63	123306.0
64	101152.5
65	83134.5
66	66451.0
67	51615.0
68	39688.0
69	30006.5
70	22573.0
71	17029.5
72	13359.0
73	10155.5
74	7661.0
75	5690.5
76	3905.5
77	2723.5
78	2021.5
79	1434.5
80	992.5
81	721.0
82	535.5
83	412.0
84	343.5
85	289.0
86	253.0
87	224.0
88	173.5
89	139.0
90	118.5
91	92.5
92	63.5
93	54.5
94	43.0
95	28.5
96	25.0
97	29.0
98	32.0
99	32.5
100	57.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.054255815958808275
2	0.015933110390735788
3	0.01871846994330246
4	0.01578370413121114
5	0.015239438471514202
6	0.014182922779161326
7	0.02196272015012341
8	0.015196750968792874
9	0.015079360336309222
10-11	0.017790016759113566
12-13	0.01944415748956504
14-15	0.015516907239202836
16-17	0.016354649480108906
18-19	0.014481735298210627
20-21	0.017304446415658457
22-23	0.017768673007752905
24-25	0.01853171211889665
26-27	0.011883133570049765
28-29	0.016354649480108906
30-31	0.015564930679764331
32-33	0.016370657293629402
34-35	0.014193594654841659
36-37	0.014695172811817267
38-39	0.02046865755487692
40-41	0.016386665107149898
42-43	0.013403875854497085
44-45	0.02151983730938963
46-47	0.019721626257253676
48-49	0.019209376224597733
50-51	0.014620469682054944
52-53	0.015266118160715034
54-55	0.015570266617604496
56-57	0.020554032560319575
58-59	0.009204492774286414
60-61	0.012395383602705704
62-63	0.01776333706991274
64-65	0.02290184520999263
66-67	0.015543586928403668
68-69	0.016744172942441026
70-71	0.024379899991718624
72-73	0.02014850128446696
74-75	0.020281899730471106
76-77	0.013510594611300407
78-79	0.015015329082227228
80-81	0.020367274735913765
82-83	0.014791219692940255
84-85	0.015009993144387062
86-87	0.01861175118649914
88-89	0.017427172985982276
90-91	0.017608594872547925
92-93	0.012496766421668859
94-95	0.017661954250949585
96-97	0.009145797458044587
98-99	0.006595219170445222
100	0.004802344056149433
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9370424.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.64364044732465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54859564075193	38.71865824317414
2	14.59249068551913	15.364036657588063
3	4.707253910631173	7.434209470965911
4	2.2398741114117056	4.716605094737145
5	1.2358142718328176	3.2528881093019586
6	0.765856624442687	2.4190488462817537
7	0.5295133616225132	1.9512857714916847
8	0.3794845350634326	1.5981957935359608
9	0.28431430408094344	1.3470605998261662
>10	1.5646039388432742	14.850209859907446
>50	0.10485586830369958	3.7978959731856774
>100	0.046243576489465775	4.057423727610398
>500	9.161370007607799E-4	0.31488762956588395
>1k	1.6269693722045341E-4	0.12123692552809583
>5k	2.0337069032795775E-5	0.05635729729952146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0671875680332074E-5	0.0
5	0.0	0.0	0.0	1.0671875680332074E-5	0.0
6	0.0	0.0	0.0	1.0671875680332074E-5	0.0
7	0.0	0.0	0.0	1.0671875680332074E-5	0.0
8	0.0	0.0	0.0	1.0671875680332074E-5	0.0
9	1.0671875680332074E-5	0.0	0.0	6.403125408199245E-5	0.0
10-11	1.0671875680332074E-5	1.0671875680332074E-5	0.0	6.936719192215848E-5	0.0
12-13	1.0671875680332074E-5	1.0671875680332074E-5	0.0	7.470312976232452E-5	0.0
14-15	1.0671875680332074E-5	3.2015627040996225E-5	0.0	7.470312976232452E-5	0.0
16-17	1.0671875680332074E-5	4.8023440561494334E-5	0.0	7.470312976232452E-5	0.0
18-19	1.0671875680332074E-5	5.335937840166037E-5	0.0	8.53750054426566E-5	0.0
20-21	1.0671875680332074E-5	5.335937840166037E-5	0.0	1.1205469464348678E-4	0.0
22-23	1.0671875680332074E-5	5.335937840166037E-5	0.0	2.401172028074717E-4	0.0
24-25	1.0671875680332074E-5	8.003906760249057E-5	0.0	5.816172245780981E-4	5.335937840166037E-6
26-27	1.0671875680332074E-5	9.604688112298867E-5	0.0	0.0011578985113160302	1.0671875680332074E-5
28-29	2.134375136066415E-5	1.0671875680332074E-4	0.0	0.002699984547124015	1.0671875680332074E-5
30-31	2.134375136066415E-5	1.0671875680332074E-4	0.0	0.006563203543404226	1.0671875680332074E-5
32-33	3.2015627040996225E-5	1.1739063248365283E-4	0.0	0.015084696274149389	1.0671875680332074E-5
34-35	3.735156488116226E-5	1.3873438384431696E-4	0.0	0.027821579898625718	1.0671875680332074E-5
36-37	4.26875027213283E-5	1.6541407304514715E-4	0.0	0.048973237497043895	1.0671875680332074E-5
38-39	5.335937840166038E-5	1.9742970008614338E-4	0.0	0.0813357004976509	1.0671875680332074E-5
40-41	6.403125408199245E-5	2.0276563792630942E-4	0.0	0.11821236691103838	1.0671875680332074E-5
42-43	6.403125408199245E-5	2.667968920083019E-4	0.0	0.15708467407664797	1.0671875680332074E-5
44-45	6.403125408199245E-5	2.667968920083019E-4	0.0	0.20366207548345733	1.0671875680332074E-5
46-47	7.470312976232452E-5	2.721328298484679E-4	0.0	0.26048447754338544	1.0671875680332074E-5
48-49	7.470312976232452E-5	2.88140643368966E-4	0.0	0.32348589562222585	1.0671875680332074E-5
50-51	7.470312976232452E-5	2.88140643368966E-4	0.0	0.39078274366239996	1.0671875680332074E-5
52-53	7.470312976232452E-5	2.988125190492981E-4	0.0	0.4583410526567421	1.0671875680332074E-5
54-55	7.470312976232452E-5	3.0414845688946416E-4	0.0	0.5339619637275752	1.0671875680332074E-5
56-57	7.470312976232452E-5	3.308281460902943E-4	0.0	0.6248596648348036	1.0671875680332074E-5
58-59	7.470312976232452E-5	3.308281460902943E-4	0.0	0.7261784525438764	1.0671875680332074E-5
60-61	7.470312976232452E-5	3.415000217706264E-4	0.0	0.8319580842873279	1.0671875680332074E-5
62-63	8.53750054426566E-5	3.415000217706264E-4	0.0	0.9402722865048583	1.0671875680332074E-5
64-65	8.53750054426566E-5	3.5217189745095846E-4	0.0	1.0573534345937814	1.0671875680332074E-5
66-67	9.604688112298867E-5	3.5217189745095846E-4	0.0	1.1920164978660517	1.0671875680332074E-5
68-69	9.604688112298867E-5	3.7351564881162264E-4	0.0	1.3418549683557544	1.0671875680332074E-5
70-71	9.604688112298867E-5	3.8952346233212074E-4	0.0	1.503464517720863	1.0671875680332074E-5
72-73	9.604688112298867E-5	4.2153908937311694E-4	0.0	1.675084286474123	1.0671875680332074E-5
74-75	9.604688112298867E-5	4.2687502721328296E-4	0.0	1.848422227211917	1.0671875680332074E-5
76-77	9.604688112298867E-5	4.2687502721328296E-4	0.0	2.035948426666712	1.0671875680332074E-5
78-79	9.604688112298867E-5	4.2687502721328296E-4	0.0	2.2447223306010486	1.0671875680332074E-5
80-81	9.604688112298867E-5	4.2687502721328296E-4	0.0	2.4725295248112573	1.0671875680332074E-5
82-83	9.604688112298867E-5	4.2687502721328296E-4	0.0	2.709050305514457	1.0671875680332074E-5
84-85	9.604688112298867E-5	4.2687502721328296E-4	0.0	2.9468997347398584	1.0671875680332074E-5
86-87	1.0671875680332074E-4	4.3754690289361505E-4	0.0	3.1955917896564765	1.0671875680332074E-5
88	1.0671875680332074E-4	4.3754690289361505E-4	0.0	3.3992912167048153	1.0671875680332074E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8795	0.0	35.448288	1
GTATCAA	13885	0.0	31.868479	1
TACCTGG	8245	0.0	30.38517	2
TATAACG	1015	0.0	30.100412	2
GTACCTG	9040	0.0	28.713638	1
TCAACGC	14595	0.0	28.564466	4
ATCAACG	14855	0.0	27.93915	3
TAACGCA	1270	0.0	27.386377	4
CAACGCA	15910	0.0	26.44129	5
AACGCAG	16750	0.0	25.254913	6
TATCAAC	17275	0.0	24.542208	2
ATAACGC	1455	0.0	23.259192	3
GTACATG	29505	0.0	23.23697	1
TACATGG	30510	0.0	21.537241	2
ACCTGGG	11430	0.0	21.465916	3
ACGCAGA	19730	0.0	21.345259	7
ACATGGG	30580	0.0	20.627193	3
GTATAAC	2760	0.0	20.6155	1
TATCACG	1570	0.0	20.357967	2
CGCAGAG	20705	0.0	20.22574	8
>>END_MODULE