FastQCFastQC Report
Wed 25 May 2016
SRR2049433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9370424
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248440.2651320794021701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219120.2338421399074364No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151310.1614761509191046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127050.135586180518619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137550.051.5407681
TATAACG11750.044.0018042
GTATCAA240600.038.303371
ATAACGC16000.034.370163
TAACGCA15400.033.5728074
TCAACGC284700.031.8298974
ATCAACG284050.031.7869033
TACCTGG87550.031.1914562
CAACGCA300500.030.281445
GTACCTG94400.029.9004331
TATCAAC304250.029.7846242
AACGCAG315250.029.0206896
ACGCAGA363850.025.2089587
CGCAGAG374550.024.3883828
ACCTGGG112350.023.971613
GTATAAC31800.023.669611
GTACATG283250.021.557731
GCAGAGT426050.021.3519769
GTGGTAT68650.020.2838041
TACATGG295550.020.0062332
Produced by FastQC (version 0.11.5)