##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049433_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9370424 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82748016525186 31.0 31.0 34.0 30.0 34.0 2 32.011821450128615 33.0 31.0 34.0 30.0 34.0 3 32.03638800122599 34.0 31.0 34.0 30.0 34.0 4 35.71897301552203 37.0 35.0 37.0 33.0 37.0 5 35.775941088684995 37.0 35.0 37.0 35.0 37.0 6 35.82825142170728 37.0 35.0 37.0 35.0 37.0 7 35.78660869561505 37.0 35.0 37.0 33.0 37.0 8 35.799792197236755 37.0 35.0 37.0 35.0 37.0 9 37.526489943251235 39.0 37.0 39.0 35.0 39.0 10-11 37.48303043704319 39.0 37.0 39.0 35.0 39.0 12-13 37.44813046880269 39.0 37.0 39.0 34.5 39.0 14-15 38.73999058100252 40.0 38.0 41.0 35.0 41.0 16-17 38.74620684186756 40.0 38.0 41.0 35.0 41.0 18-19 38.795150198112694 40.0 38.0 41.0 35.0 41.0 20-21 38.802420306701165 40.0 38.0 41.0 35.0 41.0 22-23 38.76655933605566 40.0 38.0 41.0 34.5 41.0 24-25 38.73824268784422 40.0 38.0 41.0 34.0 41.0 26-27 38.63186628481273 40.0 38.0 41.0 34.0 41.0 28-29 38.54935550408391 40.0 38.0 41.0 34.0 41.0 30-31 38.4573850660333 40.0 38.0 41.0 34.0 41.0 32-33 38.34777967357721 40.0 38.0 41.0 34.0 41.0 34-35 38.242467469988554 40.0 38.0 41.0 34.0 41.0 36-37 38.13702154779762 40.0 38.0 41.0 33.5 41.0 38-39 38.05505706038488 40.0 38.0 41.0 33.0 41.0 40-41 37.97428435468875 40.0 37.0 41.0 33.0 41.0 42-43 37.85150832022116 40.0 37.0 41.0 33.0 41.0 44-45 37.6949572932879 40.0 37.0 41.0 33.0 41.0 46-47 37.48788784797785 40.0 37.0 41.0 33.0 41.0 48-49 37.281654597486735 40.0 37.0 41.0 32.0 41.0 50-51 37.121215859602515 40.0 36.0 41.0 32.0 41.0 52-53 36.98226456988499 39.5 36.0 41.0 31.5 41.0 54-55 36.81548855206552 39.0 35.5 41.0 31.0 41.0 56-57 36.63657807800372 39.0 35.0 41.0 31.0 41.0 58-59 36.42463078511709 39.0 35.0 41.0 31.0 41.0 60-61 36.203686140563114 38.5 35.0 40.5 31.0 41.0 62-63 35.95583828437219 38.0 35.0 40.0 30.0 41.0 64-65 35.705057156431764 37.5 35.0 40.0 30.0 41.0 66-67 35.4115831364728 37.0 35.0 40.0 30.0 41.0 68-69 35.097718523729554 36.5 34.0 39.5 29.5 41.0 70-71 34.78454496829599 36.0 34.0 39.0 29.0 41.0 72-73 34.45908248121963 35.5 34.0 39.0 29.0 40.5 74-75 34.144082274185244 35.0 34.0 37.5 29.0 40.0 76-77 33.836986618748526 35.0 34.0 37.0 29.0 39.0 78-79 33.54415307140851 35.0 34.0 37.0 29.0 39.0 80-81 33.26269857159078 35.0 34.0 36.0 29.0 39.0 82-83 33.015018957519956 35.0 33.5 36.0 28.5 37.5 84-85 32.773731583544134 35.0 33.0 35.5 28.0 37.0 86-87 32.56539037080926 35.0 33.0 35.0 27.5 37.0 88-89 32.375284939080665 35.0 33.0 35.0 27.0 36.0 90-91 32.20557970482446 35.0 33.0 35.0 27.0 36.0 92-93 32.052459205688024 35.0 33.0 35.0 27.0 36.0 94-95 31.902034742504718 35.0 33.0 35.0 27.0 35.5 96-97 31.74152140820949 35.0 33.0 35.0 26.0 35.0 98-99 31.599406921180943 35.0 33.0 35.0 26.0 35.0 100 31.487841425318642 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22639307890911908 1101 2 0.26054701806643266 1101 3 0.39680250041295295 1101 4 0.23873036357797162 1101 5 0.2271098676819321 1101 6 0.19463540768172294 1101 7 0.16168946873445122 1101 8 0.1838033364561369 1101 9 0.23359475194336454 1101 10-11 0.2670129621558175 1101 12-13 0.22546126759070972 1101 14-15 0.31440233049132615 1101 16-17 0.32049244025296986 1101 18-19 0.34191845898774886 1101 20-21 0.34301574374153887 1101 22-23 0.3237099197889819 1101 24-25 0.327200361716649 1101 26-27 0.346042832627667 1101 28-29 0.3679950435798034 1101 30-31 0.37231882413090744 1101 32-33 0.36661171673136295 1101 34-35 0.3570659942222534 1101 36-37 0.39834107060379154 1101 38-39 0.39368330050175615 1101 40-41 0.4076283254584183 1101 42-43 0.4247434993298711 1101 44-45 0.40442372270846505 1101 46-47 0.39937660371694506 1101 48-49 0.4674948736725639 1101 50-51 0.40886453468871053 1101 52-53 0.4039132910000731 1101 54-55 0.37792719548633613 1101 56-57 0.4409552997293176 1101 58-59 0.43734798167802325 1101 60-61 0.3968618405993567 1101 62-63 0.39332920889020784 1101 64-65 0.36127535978194913 1101 66-67 0.38344965505841344 1101 68-69 0.39038050480068165 1101 70-71 0.3794256434147485 1101 72-73 0.39137160321548237 1101 74-75 0.3451892384366033 1101 76-77 0.37171807424956427 1101 78-79 0.3828578043745665 1101 80-81 0.3775250408682922 1101 82-83 0.3232077227674779 1101 84-85 0.36362575927788043 1101 86-87 0.3160672492912511 1101 88-89 0.35179104636648617 1101 90-91 0.343819162363296 1101 92-93 0.37070259067680666 1101 94-95 0.3853626077414525 1101 96-97 0.33917987468560895 1101 98-99 0.4073843281485665 1101 100 0.3920158746433664 1102 1 0.2128948419886072 1102 2 0.23403277964774816 1102 3 0.33538505394243145 1102 4 0.21914907052089205 1102 5 0.2087544602502618 1102 6 0.14028670042316804 1102 7 0.16004534585322006 1102 8 0.1825672033208292 1102 9 0.18890218260500546 1102 10-11 0.21704167440478273 1102 12-13 0.20991956473812223 1102 14-15 0.283534822491859 1102 16-17 0.3079013202527463 1102 18-19 0.31299292870364326 1102 20-21 0.2695410145857764 1102 22-23 0.27526491142420895 1102 24-25 0.25308160582608963 1102 26-27 0.26104326671241296 1102 28-29 0.30830914513513363 1102 30-31 0.292724056573725 1102 32-33 0.30231276055958034 1102 34-35 0.33509405923710744 1102 36-37 0.329071200003618 1102 38-39 0.3341535187966116 1102 40-41 0.3415944216809379 1102 42-43 0.26020961526421615 1102 44-45 0.31130589699458966 1102 46-47 0.3129137237981894 1102 48-49 0.30040042032548797 1102 50-51 0.27845599751002226 1102 52-53 0.29456401780471 1102 54-55 0.24270264136016806 1102 56-57 0.25084123834582783 1102 58-59 0.2525876647091181 1102 60-61 0.2744115247703576 1102 62-63 0.2633818469518374 1102 64-65 0.2573933167224709 1102 66-67 0.2236200895085929 1102 68-69 0.2367881957393223 1102 70-71 0.23358744320752578 1102 72-73 0.2634829379006831 1102 74-75 0.22744103785143466 1102 76-77 0.20150030496755988 1102 78-79 0.22228132100863718 1102 80-81 0.23596690882456528 1102 82-83 0.2667145117685976 1102 84-85 0.23085125509627602 1102 86-87 0.21058164306356275 1102 88-89 0.20524642207276145 1102 90-91 0.20199808644510142 1102 92-93 0.24078722495285376 1102 94-95 0.24310308069432196 1102 96-97 0.2618842960552321 1102 98-99 0.23170677624906943 1102 100 0.2923238983810741 1103 1 0.2047391073011653 1103 2 0.2108158734414971 1103 3 0.3194885565915371 1103 4 0.18305042197584953 1103 5 0.19741921281044483 1103 6 0.16203529678728756 1103 7 0.1433694788589861 1103 8 0.11826411052166463 1103 9 0.17827781690890276 1103 10-11 0.1767416371770878 1103 12-13 0.15749933248982728 1103 14-15 0.26451962268137663 1103 16-17 0.2208884466549179 1103 18-19 0.21823161759825638 1103 20-21 0.24377810731726157 1103 22-23 0.2667732324001193 1103 24-25 0.21858430327961997 1103 26-27 0.2496809280122605 1103 28-29 0.2736699215155127 1103 30-31 0.26025191828839667 1103 32-33 0.28673546820756 1103 34-35 0.294787584865162 1103 36-37 0.26210761614427014 1103 38-39 0.26010487415391026 1103 40-41 0.2441046261431552 1103 42-43 0.2518823348264405 1103 44-45 0.24063367817588954 1103 46-47 0.2677303639542288 1103 48-49 0.22996633164070346 1103 50-51 0.25973046919107645 1103 52-53 0.24735370621791475 1103 54-55 0.23588636312082656 1103 56-57 0.22895544516464383 1103 58-59 0.2565219174305753 1103 60-61 0.20317204435564662 1103 62-63 0.21104139937691713 1103 64-65 0.23107169019701956 1103 66-67 0.20964780928837712 1103 68-69 0.2329520362556181 1103 70-71 0.26165417125268675 1103 72-73 0.27066323250363666 1103 74-75 0.2410352587877611 1103 76-77 0.2433994310492551 1103 78-79 0.24883335585762723 1103 80-81 0.24786251934142456 1103 82-83 0.24673733738484316 1103 84-85 0.2622310624930577 1103 86-87 0.2196258335063419 1103 88-89 0.2228861505799884 1103 90-91 0.2083723787563727 1103 92-93 0.24851998124547237 1103 94-95 0.20314256829211885 1103 96-97 0.23196616796572656 1103 98-99 0.2339596772807404 1103 100 0.21787651823662557 1104 1 0.19180693250729064 1104 2 0.16427702266013 1104 3 0.2869491953527614 1104 4 0.16662666011682603 1104 5 0.13334502810042892 1104 6 0.14214991767984486 1104 7 0.1085187823497904 1104 8 0.1485722345508833 1104 9 0.1981850250316768 1104 10-11 0.17418670102073008 1104 12-13 0.16916739993119734 1104 14-15 0.25667568210923264 1104 16-17 0.2409864306132974 1104 18-19 0.2028619038985724 1104 20-21 0.2465181260517184 1104 22-23 0.22147047878066672 1104 24-25 0.22598124756219562 1104 26-27 0.24293899473103409 1104 28-29 0.25972168454925537 1104 30-31 0.22744522055675276 1104 32-33 0.23404147151824617 1104 34-35 0.18760814317299435 1104 36-37 0.20199481963977917 1104 38-39 0.21160881031094192 1104 40-41 0.1898580274800281 1104 42-43 0.24490214388884368 1104 44-45 0.2341972609505376 1104 46-47 0.2221220120753813 1104 48-49 0.20796387481578904 1104 50-51 0.19653093536465605 1104 52-53 0.18245147472349288 1104 54-55 0.164305731068481 1104 56-57 0.1561060387205515 1104 58-59 0.21793898169053705 1104 60-61 0.1823368591225787 1104 62-63 0.16833114892794754 1104 64-65 0.1041383022381126 1104 66-67 0.15370326220000408 1104 68-69 0.12897993242194872 1104 70-71 0.1503016486980897 1104 72-73 0.19157883135305553 1104 74-75 0.18459218175045322 1104 76-77 0.15747433835482383 1104 78-79 0.14527058053380415 1104 80-81 0.16242468145341604 1104 82-83 0.13838574757516398 1104 84-85 0.15833091591885307 1104 86-87 0.16577063237618006 1104 88-89 0.13649837663174225 1104 90-91 0.12658815109501376 1104 92-93 0.19700754630393646 1104 94-95 0.20158259982114757 1104 96-97 0.20212315417211002 1104 98-99 0.24982910359952726 1104 100 0.24004344841864622 1105 1 0.18701922002443183 1105 2 0.18453030129893477 1105 3 0.30316900381878753 1105 4 0.18382979663013543 1105 5 0.18954319530625696 1105 6 0.15342250642833477 1105 7 0.16005102909841185 1105 8 0.14357787984483394 1105 9 0.17420320484516338 1105 10-11 0.1864563530614447 1105 12-13 0.16242529975294673 1105 14-15 0.23254307069088043 1105 16-17 0.2415705729481772 1105 18-19 0.20277268925477188 1105 20-21 0.2164363928370392 1105 22-23 0.2085640790356038 1105 24-25 0.2624248018280255 1105 26-27 0.2219804236378593 1105 28-29 0.23954441378624836 1105 30-31 0.26572314669046904 1105 32-33 0.22359465250223565 1105 34-35 0.2686233767919006 1105 36-37 0.2534245341253296 1105 38-39 0.21980378128814237 1105 40-41 0.23656921356095495 1105 42-43 0.2802989657624906 1105 44-45 0.266664634523444 1105 46-47 0.28299209859633834 1105 48-49 0.2725335810163756 1105 50-51 0.2365616494140994 1105 52-53 0.2557956049699115 1105 54-55 0.3129677601822962 1105 56-57 0.2748766894409229 1105 58-59 0.272680436972216 1105 60-61 0.2528821779935839 1105 62-63 0.24853320861145534 1105 64-65 0.247575986053036 1105 66-67 0.2625965961386427 1105 68-69 0.3000015997966514 1105 70-71 0.29412773987345986 1105 72-73 0.3139069130948755 1105 74-75 0.31714692158679014 1105 76-77 0.3323742712434168 1105 78-79 0.2574242692629838 1105 80-81 0.2795805540813916 1105 82-83 0.2431876412859708 1105 84-85 0.22739891999827933 1105 86-87 0.23368559254564047 1105 88-89 0.25370094599905 1105 90-91 0.24753847536976537 1105 92-93 0.2620367309926266 1105 94-95 0.1788606815848901 1105 96-97 0.2361319274567073 1105 98-99 0.2637030518433825 1105 100 0.23070175463144693 1106 1 0.1468914908284802 1106 2 0.17025604023771024 1106 3 0.26262549535060487 1106 4 0.18094118813097992 1106 5 0.13352776553608692 1106 6 0.11514837557614754 1106 7 0.14115428678076825 1106 8 0.09169856319557113 1106 9 0.13381692277095425 1106 10-11 0.12688531953635618 1106 12-13 0.1408636142860047 1106 14-15 0.2062271911637552 1106 16-17 0.16079946960759628 1106 18-19 0.17015568840345452 1106 20-21 0.1638033347252943 1106 22-23 0.15820686097176662 1106 24-25 0.19048861122869454 1106 26-27 0.16950426336468638 1106 28-29 0.18718902794949344 1106 30-31 0.2133935650890848 1106 32-33 0.18350799778555427 1106 34-35 0.21264448743652054 1106 36-37 0.24955594597236086 1106 38-39 0.19575081694510033 1106 40-41 0.2104850338268065 1106 42-43 0.19116494730860012 1106 44-45 0.21256033567431132 1106 46-47 0.2060750258773254 1106 48-49 0.18152853056390938 1106 50-51 0.1708492205071721 1106 52-53 0.11246572433257285 1106 54-55 0.18552783704201659 1106 56-57 0.1719478202689544 1106 58-59 0.14766665786435595 1106 60-61 0.1439501164762902 1106 62-63 0.11600409380135801 1106 64-65 0.10199386322729964 1106 66-67 0.1159073781035147 1106 68-69 0.14346547796143483 1106 70-71 0.1821309313089401 1106 72-73 0.15432362698085456 1106 74-75 0.15344000998426566 1106 76-77 0.15073377999546977 1106 78-79 0.17443799994683218 1106 80-81 0.14113708577784223 1106 82-83 0.14505298674882994 1106 84-85 0.15867051177436764 1106 86-87 0.1955331207020805 1106 88-89 0.15802900623730665 1106 90-91 0.16471900604637568 1106 92-93 0.153935401576625 1106 94-95 0.12536448317301208 1106 96-97 0.15135099252553275 1106 98-99 0.15380123127243905 1106 100 0.15967490174834253 1107 1 0.06721220338222977 1107 2 0.08325225298082017 1107 3 0.1976375386705982 1107 4 0.09450856839825406 1107 5 0.08720419369327459 1107 6 0.09869157683372265 1107 7 0.03905586081006618 1107 8 0.05504220021961004 1107 9 0.06996400591967245 1107 10-11 0.10353882153476945 1107 12-13 0.07377261315046013 1107 14-15 0.1080083694375702 1107 16-17 0.11092419849508417 1107 18-19 0.11174264896070696 1107 20-21 0.10195272473270478 1107 22-23 0.08705007602519998 1107 24-25 0.09170243098680686 1107 26-27 0.14641267344681097 1107 28-29 0.16908416758516154 1107 30-31 0.2004848229726619 1107 32-33 0.1953996973949259 1107 34-35 0.1376776635526511 1107 36-37 0.14154109193187026 1107 38-39 0.13968737703998357 1107 40-41 0.1452478875419061 1107 42-43 0.14952958205051203 1107 44-45 0.15589287740927915 1107 46-47 0.16979955878213815 1107 48-49 0.15492812624287922 1107 50-51 0.1401676076289604 1107 52-53 0.17325185302620838 1107 54-55 0.14368998846932612 1107 56-57 0.12571462671182587 1107 58-59 0.11902031441813676 1107 60-61 0.10421098935800899 1107 62-63 0.11192783149247987 1107 64-65 0.08224624008334303 1107 66-67 0.11957518012287949 1107 68-69 0.11133066875898834 1107 70-71 0.12955389374020143 1107 72-73 0.13856539890179675 1107 74-75 0.048531851783380375 1107 76-77 0.0809961536364554 1107 78-79 0.10764721025843471 1107 80-81 0.10930087651895803 1107 82-83 0.09213546007558904 1107 84-85 0.07484659354518897 1107 86-87 0.062365444474352216 1107 88-89 0.05500284088183349 1107 90-91 0.057997913763784936 1107 92-93 0.08966782176100452 1107 94-95 0.08642084289119722 1107 96-97 0.0456342270183292 1107 98-99 0.08941298733260794 1107 100 0.08732877997945465 1108 1 0.044048629436737485 1108 2 0.13041355870775817 1108 3 0.1874156938661926 1108 4 0.10942274027602394 1108 5 0.07452507468060787 1108 6 0.06611451007438518 1108 7 0.06172535830569359 1108 8 0.026052189924136826 1108 9 0.08860906619785425 1108 10-11 0.07038445642767499 1108 12-13 0.07341561194011348 1108 14-15 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-0.3647152108972378 2215 3 -0.27432987075957627 2215 4 -0.2396531411510594 2215 5 -0.25872199056735923 2215 6 -0.21317607441749686 2215 7 -0.24443186410167073 2215 8 -0.21542615804173693 2215 9 -0.35749632204758086 2215 10-11 -0.36814339516142525 2215 12-13 -0.36791722954236405 2215 14-15 -0.49563748705120503 2215 16-17 -0.483548759734056 2215 18-19 -0.48395700027857913 2215 20-21 -0.4394454924724087 2215 22-23 -0.4308658347105663 2215 24-25 -0.4263860992925217 2215 26-27 -0.4389401438852616 2215 28-29 -0.43551353886369526 2215 30-31 -0.41287206565584 2215 32-33 -0.41354709945047574 2215 34-35 -0.42990068678411575 2215 36-37 -0.4710687300488061 2215 38-39 -0.4521669015480967 2215 40-41 -0.48218117680308126 2215 42-43 -0.41566827617182156 2215 44-45 -0.42654808698126345 2215 46-47 -0.45539151101883135 2215 48-49 -0.42972799970912234 2215 50-51 -0.389738121792071 2215 52-53 -0.4455132360899441 2215 54-55 -0.4212527111729969 2215 56-57 -0.3722022478392688 2215 58-59 -0.365885228936051 2215 60-61 -0.42115459907594754 2215 62-63 -0.42126763673130796 2215 64-65 -0.43082331521907236 2215 66-67 -0.41143183454259 2215 68-69 -0.4155166265589685 2215 70-71 -0.38643838212526305 2215 72-73 -0.33169435591369023 2215 74-75 -0.30238912425197384 2215 76-77 -0.2536632490680901 2215 78-79 -0.28236552242255897 2215 80-81 -0.36409053162729776 2215 82-83 -0.35464029775165073 2215 84-85 -0.31659980893728346 2215 86-87 -0.3212269608804661 2215 88-89 -0.3436521427623873 2215 90-91 -0.35486112802595215 2215 92-93 -0.3140153468949265 2215 94-95 -0.3491821328172513 2215 96-97 -0.31508684076610294 2215 98-99 -0.2877051916843385 2215 100 -0.31219087115013266 2216 1 -0.2957832313005291 2216 2 -0.35801311187178086 2216 3 -0.3341488003309898 2216 4 -0.24502246211091716 2216 5 -0.3337711361559528 2216 6 -0.2918473874858307 2216 7 -0.3622053881653784 2216 8 -0.2793432298352698 2216 9 -0.441721091950825 2216 10-11 -0.3849887320808847 2216 12-13 -0.38587000885049605 2216 14-15 -0.6405268443550085 2216 16-17 -0.578780960528114 2216 18-19 -0.5073728508593405 2216 20-21 -0.5020426314935875 2216 22-23 -0.45895891307996095 2216 24-25 -0.45763223929158414 2216 26-27 -0.5004491229775567 2216 28-29 -0.574569580371012 2216 30-31 -0.5690414141299769 2216 32-33 -0.5970162486086465 2216 34-35 -0.5855863223640938 2216 36-37 -0.6548881094668388 2216 38-39 -0.5979899790553915 2216 40-41 -0.6380371098020348 2216 42-43 -0.6926877213128435 2216 44-45 -0.7238366962882523 2216 46-47 -0.713896371931412 2216 48-49 -0.7779434167675277 2216 50-51 -0.7524530987128415 2216 52-53 -0.7244160722590536 2216 54-55 -0.7412538898036729 2216 56-57 -0.7342878615348098 2216 58-59 -0.7322455897279099 2216 60-61 -0.7120395557584658 2216 62-63 -0.6455781527905273 2216 64-65 -0.6766606764601448 2216 66-67 -0.6600430724879303 2216 68-69 -0.6670288699239038 2216 70-71 -0.6977754482149834 2216 72-73 -0.6677441632042829 2216 74-75 -0.68995317478511 2216 76-77 -0.6427076175240671 2216 78-79 -0.7014479053961793 2216 80-81 -0.7250721296363736 2216 82-83 -0.7017348405232013 2216 84-85 -0.8139775086292715 2216 86-87 -0.6904891479516451 2216 88-89 -0.7789744205219264 2216 90-91 -0.6863850438614207 2216 92-93 -0.7160681002588483 2216 94-95 -0.6973780757528623 2216 96-97 -0.7560867149073971 2216 98-99 -0.8353097884291572 2216 100 -0.849028696041433 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 557.0 3 52.0 4 100.0 5 215.0 6 557.0 7 1644.0 8 3805.0 9 6202.0 10 7428.0 11 9173.0 12 12959.0 13 17372.0 14 18137.0 15 22679.0 16 29461.0 17 41248.0 18 41417.0 19 26364.0 20 21808.0 21 22137.0 22 24622.0 23 26996.0 24 31879.0 25 40682.0 26 52599.0 27 68816.0 28 90727.0 29 118183.0 30 154034.0 31 199498.0 32 258002.0 33 336666.0 34 465042.0 35 688348.0 36 1151742.0 37 2098815.0 38 2501029.0 39 777716.0 40 1713.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.305937791875635 19.03558949371621 13.750594966985208 23.907877747422948 2 16.24332047301168 20.019254198102455 38.25764981392518 25.47977551496069 3 18.12813379629353 25.24305196861956 29.95775858168211 26.671055653404796 4 13.215411940314754 17.193012524621352 36.6344934481613 32.95708208690259 5 13.721440835922538 36.49140546221896 34.353379712758354 15.433773989100153 6 31.351663720336827 36.575274414206845 17.69469121698452 14.378370648471813 7 27.856210135208396 31.681736066585675 21.891816208103283 18.570237590102646 8 26.4161377680564 34.42567544493222 19.952913509923146 19.20527327708823 9 25.577668630576376 16.38892754479413 20.72864579020117 37.304758034428325 10-11 24.738688452091388 26.09164750709253 27.966151798467177 21.203512242348904 12-13 25.382413943120724 23.5078187339658 28.275491736823298 22.83427558609018 14-15 22.646501155070833 25.239778447521193 25.843257202637957 26.27046319477001 16-17 22.31506932424132 28.431639188986495 26.23925161973736 23.014039867034825 18-19 22.34808691687804 27.14446539452217 28.614617652306872 21.892830036292914 20-21 23.065531257051777 26.313794955755192 28.59916173461354 22.021512052579492 22-23 22.871014161152154 26.137195072496183 28.578717462518238 22.41307330383342 24-25 22.644695896485743 26.284554282761835 28.54383413677477 22.52691568397765 26-27 22.576582617245684 26.270120563972615 28.55285187605528 22.600444942726416 28-29 22.582828939796293 26.253815000547203 28.65090823385931 22.512447825797192 30-31 22.695712595289177 26.500092205005878 28.402274005957466 22.401921193747476 32-33 22.476610175786124 26.385175223183676 28.58064221447468 22.557572386555524 34-35 22.64497970662692 26.163209165519575 28.57381526032415 22.61799586752936 36-37 22.86755769363039 26.308074549672167 28.35129596864005 22.473071788057393 38-39 22.734751538419466 26.459918386730575 28.306501981636345 22.498828093213614 40-41 22.723949406750176 26.226807336277346 28.437460647441632 22.61178260953084 42-43 22.656043857240924 26.38652395134574 28.295262663476834 22.6621695279365 44-45 22.69298225814877 26.50930222990669 28.11656303998078 22.681152471963753 46-47 22.87839774063441 26.484613599948265 27.876611267501623 22.760377391915707 48-49 22.89760708034262 26.52061909029652 27.781579942102752 22.800193887258104 50-51 22.8316074570491 26.633549293312598 27.626382156851427 22.908461092786876 52-53 22.881294433120967 26.624473888689735 27.553894960547588 22.940336717641706 54-55 22.890008619151892 26.85987696484834 27.537995529865473 22.7121188861343 56-57 22.70409143337195 26.657665279889624 27.58914019538957 23.04910309134885 58-59 22.714708770509155 26.39204146623318 27.757385888646713 23.13586387461095 60-61 22.844535857418528 26.62879606864923 27.62479752770901 22.901870546223233 62-63 22.682315328311983 26.680901939365143 27.714098437353595 22.92268429496928 64-65 22.741843008512166 26.44925716984178 27.742412620575667 23.06648720107039 66-67 22.58676500218561 26.539944855954428 27.743883698057537 23.12940644380242 68-69 22.82585745178697 26.597001301923463 27.580073842362516 22.997067403927048 70-71 22.826174999655834 26.39012905202517 27.62278628879194 23.160909659527064 72-73 22.606109751893214 26.610819059055284 27.639272492723215 23.143798696328286 74-75 22.6437416772372 26.52219983253625 27.56651113411977 23.267547356106782 76-77 22.710137041152045 26.57206519638389 27.408635941465448 23.309161820998618 78-79 22.740680894483013 26.626674378260734 27.519041132752704 23.11360359450355 80-81 22.562969417541392 26.532000366049303 27.564558210243028 23.340472006166276 82-83 22.570086114735872 26.516823415567032 27.604168361952425 23.30892210774467 84-85 22.592455812916565 26.524891064059258 27.401757286225774 23.480895836798403 86-87 22.633466309355637 26.61522859151318 27.385711180177587 23.365593918953603 88-89 22.48306222036055 26.505067860506436 27.510561266551587 23.50130865258142 90-91 22.399827924188248 26.45908927990851 27.50349682403055 23.637585971872692 92-93 22.501826644902206 26.614663114814153 27.377016893177036 23.50649334710661 94-95 22.43107702556135 26.367411258512153 27.530855804827155 23.670655911099338 96-97 22.34850016931171 26.450389854487977 27.462583605994734 23.73852637020558 98-99 22.265847237339493 26.539933092578742 27.460238300726097 23.733981369355668 100 22.365630482372474 26.338001699612175 27.436538545477323 23.85982927253803 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2485.0 1 1861.0 2 6816.0 3 9939.5 4 4684.0 5 1809.5 6 2838.0 7 6648.5 8 11587.0 9 13330.5 10 9554.5 11 5359.5 12 4499.5 13 4811.0 14 5412.5 15 6116.0 16 6787.0 17 7514.0 18 8470.5 19 9488.0 20 10791.0 21 12802.0 22 15779.0 23 20412.5 24 26995.0 25 35758.0 26 47188.0 27 61439.5 28 78494.5 29 97252.5 30 114631.5 31 131953.5 32 149881.5 33 164867.0 34 178625.5 35 194229.5 36 212577.5 37 232756.5 38 251741.0 39 266567.0 40 280965.0 41 294857.5 42 303416.5 43 307848.0 44 311737.0 45 316184.0 46 319722.0 47 317644.5 48 314290.0 49 315346.0 50 315837.5 51 314997.5 52 307728.0 53 302797.5 54 300980.5 55 288931.5 56 271956.5 57 250248.5 58 229149.5 59 214235.0 60 195406.0 61 169840.0 62 144731.5 63 121096.0 64 98108.5 65 79992.0 66 63589.0 67 48507.5 68 36929.0 69 27430.0 70 20088.0 71 14707.0 72 11108.5 73 8237.0 74 6222.5 75 4501.5 76 2829.5 77 1866.5 78 1299.5 79 771.0 80 388.5 81 185.0 82 83.5 83 37.5 84 25.0 85 17.5 86 11.0 87 8.0 88 6.0 89 5.0 90 3.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09135125582364256 2 0.0 3 0.0 4 1.0671875680332074E-5 5 0.00802525051160972 6 9.604688112298867E-5 7 0.0 8 8.53750054426566E-5 9 0.0 10-11 0.0 12-13 0.01481256344430092 14-15 3.2015627040996225E-5 16-17 5.335937840166037E-6 18-19 0.0 20-21 2.6679689200830185E-5 22-23 0.0 24-25 4.8023440561494334E-5 26-27 4.8023440561494334E-5 28-29 1.3339844600415094E-4 30-31 0.0 32-33 3.2015627040996225E-5 34-35 5.335937840166037E-6 36-37 5.335937840166037E-6 38-39 1.2272657032381886E-4 40-41 4.26875027213283E-5 42-43 2.2944532712713958E-4 44-45 1.0138281896315471E-4 46-47 6.403125408199245E-5 48-49 3.201562704099622E-4 50-51 1.600781352049811E-4 52-53 2.241093892869736E-4 54-55 1.3339844600415094E-4 56-57 1.707500108853132E-4 58-59 4.8023440561494334E-5 60-61 6.403125408199245E-5 62-63 4.0553127585261884E-4 64-65 1.2272657032381886E-4 66-67 5.976250380985962E-4 68-69 6.136328516190943E-4 70-71 1.8142188656564528E-4 72-73 4.6422659209444523E-4 74-75 4.0553127585261884E-4 76-77 2.88140643368966E-4 78-79 0.0012539453924390188 80-81 0.0010831953815537056 82-83 8.163984895454038E-4 84-85 0.0013179766465210112 86-87 6.723281678609207E-4 88-89 0.0010565156923528754 90-91 0.001446039154684996 92-93 0.0010938672572340378 94-95 0.0015474219736481508 96-97 0.0014193594654841658 98-99 0.0017395157358941283 100 5.442656596969358E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9370424.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.63947605721259 #Duplication Level Percentage of deduplicated Percentage of total 1 71.3740293605368 35.429694215491544 2 15.607739299536163 15.495200025330828 3 5.154900597908886 7.676596944216271 4 2.4054817496654612 4.77627414874322 5 1.3610820266495138 3.378169933688547 6 0.8744027610469961 2.604293695281177 7 0.5862427658055636 2.0370548615843553 8 0.4305787407667507 1.7098962474428694 9 0.31901793548893304 1.4252294853471852 >10 1.7131777922095253 15.296924114301017 >50 0.11879609607850518 4.060291427236969 >100 0.05267436705937856 4.451773966854633 >500 0.0014238992720556516 0.45883063372450117 >1k 3.2329151792809073E-4 0.27078350219041214 >5k 4.310548590290949E-5 0.1309517046072138 >10k+ 8.621097180581898E-5 0.7980350939592559 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24844 0.2651320794021701 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21912 0.2338421399074364 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15131 0.1614761509191046 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12705 0.135586180518619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0671875680332074E-5 0.0 2 0.0 0.0 0.0 1.0671875680332074E-5 0.0 3 0.0 0.0 0.0 1.0671875680332074E-5 0.0 4 0.0 0.0 0.0 1.0671875680332074E-5 0.0 5 0.0 0.0 0.0 1.0671875680332074E-5 0.0 6 0.0 0.0 0.0 1.0671875680332074E-5 0.0 7 0.0 0.0 0.0 1.0671875680332074E-5 0.0 8 0.0 0.0 0.0 1.0671875680332074E-5 0.0 9 0.0 0.0 0.0 2.134375136066415E-5 0.0 10-11 0.0 5.335937840166037E-6 0.0 2.134375136066415E-5 0.0 12-13 0.0 1.0671875680332074E-5 0.0 2.134375136066415E-5 0.0 14-15 0.0 2.134375136066415E-5 0.0 2.134375136066415E-5 0.0 16-17 5.335937840166037E-6 3.2015627040996225E-5 0.0 3.2015627040996225E-5 0.0 18-19 1.0671875680332074E-5 4.26875027213283E-5 0.0 4.8023440561494334E-5 5.335937840166037E-6 20-21 1.0671875680332074E-5 4.26875027213283E-5 0.0 8.003906760249057E-5 1.0671875680332074E-5 22-23 1.0671875680332074E-5 4.26875027213283E-5 0.0 2.0810157576647546E-4 1.0671875680332074E-5 24-25 1.0671875680332074E-5 5.335937840166038E-5 0.0 5.602734732174339E-4 1.0671875680332074E-5 26-27 1.0671875680332074E-5 6.403125408199245E-5 0.0 0.0011258828842750338 1.0671875680332074E-5 28-29 1.0671875680332074E-5 8.003906760249057E-5 0.0 0.002694648609283849 1.0671875680332074E-5 30-31 1.0671875680332074E-5 1.0138281896315471E-4 0.0 0.0065685394812443915 1.0671875680332074E-5 32-33 1.0671875680332074E-5 1.1205469464348678E-4 0.0 0.015063352522788724 1.0671875680332074E-5 34-35 1.0671875680332074E-5 1.2272657032381886E-4 0.0 0.02791229084190854 1.0671875680332074E-5 36-37 1.0671875680332074E-5 1.6007813520498113E-4 0.0 0.049224026575531696 1.0671875680332074E-5 38-39 1.0671875680332074E-5 1.9209376224597733E-4 0.0 0.08161316926533954 1.0671875680332074E-5 40-41 1.0671875680332074E-5 1.9209376224597733E-4 0.0 0.11861256224905084 1.0671875680332074E-5 42-43 1.0671875680332074E-5 1.9209376224597733E-4 0.0 0.1576289397363449 1.0671875680332074E-5 44-45 1.0671875680332074E-5 1.9209376224597733E-4 0.0 0.20454250522708473 1.0671875680332074E-5 46-47 1.0671875680332074E-5 2.2410938928697357E-4 0.0 0.26187182138182863 1.0671875680332074E-5 48-49 1.0671875680332074E-5 2.2410938928697357E-4 0.0 0.32497462227963214 1.0671875680332074E-5 50-51 1.0671875680332074E-5 2.507890784878038E-4 0.0 0.39221277500356444 1.0671875680332074E-5 52-53 1.0671875680332074E-5 2.614609541681358E-4 0.0 0.45995250588447223 1.0671875680332074E-5 54-55 1.0671875680332074E-5 2.774687676886339E-4 0.0 0.5356161044580267 1.0671875680332074E-5 56-57 1.6007813520498112E-5 2.88140643368966E-4 0.0 0.627026055597911 1.0671875680332074E-5 58-59 2.134375136066415E-5 2.88140643368966E-4 0.0 0.7287450386449963 1.0671875680332074E-5 60-61 2.134375136066415E-5 2.88140643368966E-4 0.0 0.834983561042702 1.0671875680332074E-5 62-63 2.134375136066415E-5 2.93476581209132E-4 0.0 0.9431643648142283 1.0671875680332074E-5 64-65 2.134375136066415E-5 2.988125190492981E-4 0.0 1.06052298167084 1.0671875680332074E-5 66-67 2.134375136066415E-5 2.988125190492981E-4 0.0 1.1952340683836717 1.0671875680332074E-5 68-69 2.134375136066415E-5 3.148203325697962E-4 0.0 1.3452059373193785 1.0671875680332074E-5 70-71 2.134375136066415E-5 3.308281460902943E-4 0.0 1.5071036273278562 1.0671875680332074E-5 72-73 2.134375136066415E-5 3.415000217706264E-4 0.0 1.6787820913973583 1.0671875680332074E-5 74-75 2.134375136066415E-5 3.5217189745095846E-4 0.0 1.852637618105648 1.0671875680332074E-5 76-77 2.134375136066415E-5 3.7351564881162264E-4 0.0 2.040537333209255 1.0671875680332074E-5 78-79 2.134375136066415E-5 3.8952346233212074E-4 0.0 2.249855502803288 1.0671875680332074E-5 80-81 2.667968920083019E-5 4.0553127585261884E-4 1.0671875680332074E-5 2.4779134860919845 1.0671875680332074E-5 82-83 3.2015627040996225E-5 4.0553127585261884E-4 1.0671875680332074E-5 2.714354227727582 1.0671875680332074E-5 84-85 3.2015627040996225E-5 4.162031515329509E-4 1.0671875680332074E-5 2.9528919929343647 1.0671875680332074E-5 86-87 3.2015627040996225E-5 4.2687502721328296E-4 1.0671875680332074E-5 3.2020856260079587 1.0671875680332074E-5 88 3.2015627040996225E-5 4.2687502721328296E-4 1.0671875680332074E-5 3.406420029659277 1.0671875680332074E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13755 0.0 51.540768 1 TATAACG 1175 0.0 44.001804 2 GTATCAA 24060 0.0 38.30337 1 ATAACGC 1600 0.0 34.37016 3 TAACGCA 1540 0.0 33.572807 4 TCAACGC 28470 0.0 31.829897 4 ATCAACG 28405 0.0 31.786903 3 TACCTGG 8755 0.0 31.191456 2 CAACGCA 30050 0.0 30.28144 5 GTACCTG 9440 0.0 29.900433 1 TATCAAC 30425 0.0 29.784624 2 AACGCAG 31525 0.0 29.020689 6 ACGCAGA 36385 0.0 25.208958 7 CGCAGAG 37455 0.0 24.388382 8 ACCTGGG 11235 0.0 23.97161 3 GTATAAC 3180 0.0 23.66961 1 GTACATG 28325 0.0 21.55773 1 GCAGAGT 42605 0.0 21.351976 9 GTGGTAT 6865 0.0 20.283804 1 TACATGG 29555 0.0 20.006233 2 >>END_MODULE