FastQCFastQC Report
Wed 25 May 2016
SRR2049432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10829927
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100300.036.846911
GTATCAA162100.033.357561
TCAACGC168600.030.8046044
ATCAACG169350.030.4744643
CAACGCA182100.028.547245
TACCTGG94700.027.6951142
AACGCAG192100.027.3788786
GTACCTG101350.026.908141
TATCAAC195350.026.4906642
TATAACG11700.025.710652
GTACATG337350.023.6387541
ACGCAGA227200.023.107657
TACATGG345300.022.1739712
CGCAGAG240750.021.7077758
ATAACGC16050.021.0851353
ACATGGG352750.020.8129183
ACCTGGG124850.020.4799693
TAACGCA15250.020.3415954
TATCACG17050.017.6430852
GCAGAGT291300.017.4569999
Produced by FastQC (version 0.11.5)