##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10829927 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.515657030744528 31.0 31.0 34.0 27.0 34.0 2 30.647881098367513 31.0 31.0 34.0 27.0 34.0 3 30.732361815550558 31.0 31.0 34.0 27.0 34.0 4 34.31303747476784 37.0 35.0 37.0 30.0 37.0 5 34.31339214013169 37.0 35.0 37.0 30.0 37.0 6 34.343688004545186 37.0 35.0 37.0 30.0 37.0 7 34.210669748743456 37.0 35.0 37.0 30.0 37.0 8 34.204207193640364 37.0 35.0 37.0 30.0 37.0 9 35.746675393102834 39.0 37.0 39.0 30.0 39.0 10-11 35.70949236315259 39.0 37.0 39.0 30.0 39.0 12-13 35.67275014873138 39.0 37.0 39.0 30.0 39.0 14-15 36.86173891107484 40.0 37.0 41.0 30.0 41.0 16-17 36.82833951696996 40.0 37.0 41.0 30.0 41.0 18-19 36.81816904213666 40.0 37.0 41.0 30.0 41.0 20-21 36.856718840302435 40.0 37.0 41.0 30.0 41.0 22-23 36.79439893731509 40.0 37.0 41.0 30.0 41.0 24-25 36.76748236622463 40.0 37.0 41.0 30.0 41.0 26-27 36.73894999476913 40.0 37.0 41.0 30.0 41.0 28-29 36.68928825651364 40.0 37.0 41.0 30.0 41.0 30-31 36.62433712618746 40.0 37.0 41.0 30.0 41.0 32-33 36.57601644036936 40.0 37.0 41.0 30.0 41.0 34-35 36.49594932634356 40.0 37.0 41.0 30.0 41.0 36-37 36.39881995511142 40.0 36.5 41.0 30.0 41.0 38-39 36.2956626577446 40.0 36.0 41.0 29.5 41.0 40-41 36.17385444980377 40.0 36.0 41.0 29.0 41.0 42-43 36.05569718983332 40.0 36.0 41.0 28.5 41.0 44-45 35.8978049436529 40.0 35.5 41.0 28.0 41.0 46-47 35.75925257852616 39.5 35.0 41.0 28.0 41.0 48-49 35.62930862784209 39.0 35.0 41.0 27.0 41.0 50-51 35.480067917355306 39.0 35.0 41.0 27.0 41.0 52-53 35.33028869908357 39.0 35.0 41.0 27.0 41.0 54-55 35.159135837203706 39.0 35.0 41.0 26.0 41.0 56-57 34.97221140087094 39.0 34.5 40.0 26.0 41.0 58-59 34.75036498399297 38.0 34.0 40.0 26.0 41.0 60-61 34.512419797474166 38.0 34.0 40.0 26.0 41.0 62-63 34.237633596237536 37.5 34.0 40.0 25.0 41.0 64-65 33.97036572822698 37.0 34.0 40.0 24.5 41.0 66-67 33.70211567446392 36.5 33.5 40.0 24.0 41.0 68-69 33.391247697237475 36.0 33.0 39.0 23.0 41.0 70-71 33.07459847143937 35.5 33.0 39.0 23.0 41.0 72-73 32.72891230014754 35.0 33.0 38.5 22.0 40.0 74-75 32.41086449613188 35.0 33.0 37.5 22.0 40.0 76-77 32.09391554532178 35.0 32.0 37.0 20.5 39.0 78-79 31.777473476968034 35.0 32.0 36.5 20.0 39.0 80-81 31.464234569632833 35.0 32.0 36.0 19.5 39.0 82-83 31.184335637719443 35.0 32.0 36.0 18.0 37.0 84-85 30.923398698809326 35.0 32.0 35.0 17.5 37.0 86-87 30.66298697119565 35.0 32.0 35.0 13.5 36.5 88-89 30.44631612013636 35.0 31.5 35.0 8.5 36.0 90-91 30.253880196976397 35.0 31.0 35.0 4.5 36.0 92-93 30.04370047923684 35.0 31.0 35.0 2.0 36.0 94-95 29.83718546763981 34.5 31.0 35.0 2.0 35.5 96-97 29.612127764111428 34.0 31.0 35.0 2.0 35.0 98-99 29.411363806976723 34.0 31.0 35.0 2.0 35.0 100 29.266335497921638 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21216551992843335 1101 2 0.2522612019631296 1101 3 0.2631654665956269 1101 4 0.31520980983614777 1101 5 0.2814823602006413 1101 6 0.30527762057601393 1101 7 0.3291302003343617 1101 8 0.3534422778839783 1101 9 0.4510437816445716 1101 10-11 0.46881591826243607 1101 12-13 0.4666226140173322 1101 14-15 0.5714942323897105 1101 16-17 0.5317595829164787 1101 18-19 0.5623302052398245 1101 20-21 0.5654183792491239 1101 22-23 0.549264981998256 1101 24-25 0.5804491872485045 1101 26-27 0.552894869020534 1101 28-29 0.5362778951425256 1101 30-31 0.5300639317720126 1101 32-33 0.5377079296817584 1101 34-35 0.5794783629082687 1101 36-37 0.5419431165785369 1101 38-39 0.5784691114968368 1101 40-41 0.6151589863808766 1101 42-43 0.5586035881256279 1101 44-45 0.5483417938936057 1101 46-47 0.510224672229036 1101 48-49 0.5042264402865229 1101 50-51 0.4993455840862495 1101 52-53 0.5346724528078752 1101 54-55 0.4959776563648859 1101 56-57 0.5166649323743542 1101 58-59 0.5625510916949139 1101 60-61 0.5226023571434837 1101 62-63 0.49212638567736633 1101 64-65 0.47468106955912504 1101 66-67 0.48059613670807977 1101 68-69 0.4676584518086315 1101 70-71 0.45775273525616456 1101 72-73 0.49193273377363056 1101 74-75 0.4560396144745269 1101 76-77 0.46333745653086567 1101 78-79 0.42051051224273905 1101 80-81 0.4414676586831625 1101 82-83 0.4165680327812282 1101 84-85 0.42539789214890433 1101 86-87 0.43174118728899913 1101 88-89 0.4287681970278996 1101 90-91 0.4400072550494194 1101 92-93 0.4480857829809466 1101 94-95 0.4221106229568221 1101 96-97 0.42332984399813967 1101 98-99 0.46388931220212726 1101 100 0.44921624761306944 1102 1 0.14551422672502312 1102 2 0.22324535315724603 1102 3 0.21852067387269614 1102 4 0.2505288441907254 1102 5 0.19659080649007876 1102 6 0.2757900386420644 1102 7 0.28451516596487636 1102 8 0.30400921856452356 1102 9 0.41215237455922704 1102 10-11 0.37191407434929147 1102 12-13 0.4172223247147073 1102 14-15 0.5437271300816775 1102 16-17 0.47689529742585535 1102 18-19 0.4802458885197112 1102 20-21 0.4956663878536176 1102 22-23 0.4767648762106802 1102 24-25 0.4764199057705625 1102 26-27 0.4292159339022277 1102 28-29 0.455055032173874 1102 30-31 0.43347741470617507 1102 32-33 0.45923543616454054 1102 34-35 0.43903822489003375 1102 36-37 0.4519897280654348 1102 38-39 0.49670665286605953 1102 40-41 0.4986000555615888 1102 42-43 0.47629368538313344 1102 44-45 0.42040315701780884 1102 46-47 0.3812086628292022 1102 48-49 0.32012877031881004 1102 50-51 0.3980340727530063 1102 52-53 0.3774278927286687 1102 54-55 0.3783910802417978 1102 56-57 0.33775073131758404 1102 58-59 0.35995807607066865 1102 60-61 0.3709673752480853 1102 62-63 0.34599825874303036 1102 64-65 0.34422286328873497 1102 66-67 0.34308019317494853 1102 68-69 0.34003903649950473 1102 70-71 0.333562367427362 1102 72-73 0.29655989134224825 1102 74-75 0.2970485931135016 1102 76-77 0.2991034116164286 1102 78-79 0.29824516635408216 1102 80-81 0.30936348879978937 1102 82-83 0.29667283226032026 1102 84-85 0.29972377534638284 1102 86-87 0.27713956731926714 1102 88-89 0.27058139095365874 1102 90-91 0.28881765791560454 1102 92-93 0.2781062535668184 1102 94-95 0.26150533526580944 1102 96-97 0.2361896479397423 1102 98-99 0.24630990337424308 1102 100 0.23104217541646221 1103 1 0.13335615916571442 1103 2 0.17482769741163295 1103 3 0.1452342310392183 1103 4 0.15598525228442384 1103 5 0.16103146584038797 1103 6 0.17400005590630485 1103 7 0.16694090742866052 1103 8 0.1830669370171023 1103 9 0.26714081209699003 1103 10-11 0.264708307697461 1103 12-13 0.2692555499668785 1103 14-15 0.3178626026282316 1103 16-17 0.3189391283209204 1103 18-19 0.2930341171237245 1103 20-21 0.3014330084089494 1103 22-23 0.264494194797507 1103 24-25 0.27453874223049723 1103 26-27 0.31222732848581813 1103 28-29 0.29338853646726193 1103 30-31 0.31546276079592417 1103 32-33 0.30538983242199436 1103 34-35 0.30425867986090793 1103 36-37 0.29076959987921924 1103 38-39 0.31922100336574744 1103 40-41 0.31826207233129367 1103 42-43 0.30419624132430556 1103 44-45 0.2501056462536795 1103 46-47 0.28868199151580853 1103 48-49 0.2710778201066901 1103 50-51 0.30567484996904426 1103 52-53 0.3102279025541037 1103 54-55 0.2691235504508498 1103 56-57 0.2717133114207968 1103 58-59 0.2514309627772917 1103 60-61 0.2319506170761514 1103 62-63 0.31118012278251683 1103 64-65 0.30324327916364524 1103 66-67 0.26631570360162726 1103 68-69 0.2576230237485646 1103 70-71 0.25560589135985623 1103 72-73 0.21945993260232655 1103 74-75 0.2503000287973194 1103 76-77 0.24212491867780273 1103 78-79 0.2324061978760419 1103 80-81 0.2582901747393791 1103 82-83 0.22656997765914966 1103 84-85 0.28110241722070484 1103 86-87 0.2507424026128291 1103 88-89 0.1957914859998553 1103 90-91 0.19612223539579787 1103 92-93 0.18395746128175006 1103 94-95 0.20083761374221254 1103 96-97 0.15485839469688756 1103 98-99 0.18098927069024384 1103 100 0.1552549961074483 1104 1 0.17606916334820255 1104 2 0.21234978538815596 1104 3 0.20731979917906784 1104 4 0.2595379326072589 1104 5 0.2805684621919724 1104 6 0.30678977860807066 1104 7 0.2577459773286108 1104 8 0.23480602494340275 1104 9 0.329454555388601 1104 10-11 0.351208623032079 1104 12-13 0.3658405752521361 1104 14-15 0.46818698197994735 1104 16-17 0.445487353488474 1104 18-19 0.4079348471738271 1104 20-21 0.43983908082624623 1104 22-23 0.4228266650734085 1104 24-25 0.4519828579414593 1104 26-27 0.4354644646872572 1104 28-29 0.3929965856946822 1104 30-31 0.3551075134118946 1104 32-33 0.3529853929323892 1104 34-35 0.40218112650082816 1104 36-37 0.3907844396226565 1104 38-39 0.4426759786780181 1104 40-41 0.457221849598227 1104 42-43 0.365766901498084 1104 44-45 0.390887904197605 1104 46-47 0.3460659813291116 1104 48-49 0.2965474649095796 1104 50-51 0.3373364106791428 1104 52-53 0.32968054204025066 1104 54-55 0.29245469498953014 1104 56-57 0.27875105339285966 1104 58-59 0.36231546787180946 1104 60-61 0.3241845582695504 1104 62-63 0.30810930606715914 1104 64-65 0.3231381526930335 1104 66-67 0.3286706858302608 1104 68-69 0.2518269448541446 1104 70-71 0.2642168498393218 1104 72-73 0.2667817488462063 1104 74-75 0.22888664075817644 1104 76-77 0.25235654891828574 1104 78-79 0.21907596710214605 1104 80-81 0.24503724984825936 1104 82-83 0.2515847036386347 1104 84-85 0.2569234671640821 1104 86-87 0.24918443404902035 1104 88-89 0.219311752688931 1104 90-91 0.19741127232527944 1104 92-93 0.22443007399361647 1104 94-95 0.23824374984349816 1104 96-97 0.29450909242253687 1104 98-99 0.2977825584247604 1104 100 0.2741972233883878 1105 1 0.1981886469443097 1105 2 0.216487852914085 1105 3 0.19993128196943744 1105 4 0.23311814454353907 1105 5 0.25457238261054016 1105 6 0.2656166611091919 1105 7 0.26377730223372 1105 8 0.3006194059654632 1105 9 0.3605600578704866 1105 10-11 0.4010701890510404 1105 12-13 0.37034976988478974 1105 14-15 0.45109750501276835 1105 16-17 0.4095839115209685 1105 18-19 0.40893520855856025 1105 20-21 0.3997276515417383 1105 22-23 0.4285403973264721 1105 24-25 0.3737035793296428 1105 26-27 0.4284688066946529 1105 28-29 0.43356170733086685 1105 30-31 0.4505759782232204 1105 32-33 0.439721077701023 1105 34-35 0.44836052467680076 1105 36-37 0.41959336451181173 1105 38-39 0.48530235885763773 1105 40-41 0.5085037093846836 1105 42-43 0.4284609079029096 1105 44-45 0.4737227237739674 1105 46-47 0.44903914941438217 1105 48-49 0.43679537283798453 1105 50-51 0.38153415148055103 1105 52-53 0.35629505370084047 1105 54-55 0.3636857876056183 1105 56-57 0.3348641932790315 1105 58-59 0.313997346786941 1105 60-61 0.36346763789055103 1105 62-63 0.33306968218360566 1105 64-65 0.31380839819470907 1105 66-67 0.3496080348839783 1105 68-69 0.3096006306946961 1105 70-71 0.346797713857093 1105 72-73 0.2877738599205131 1105 74-75 0.3235480495998999 1105 76-77 0.3287138367213345 1105 78-79 0.3412896297842458 1105 80-81 0.3296566128798588 1105 82-83 0.3155631061640989 1105 84-85 0.3259786362867132 1105 86-87 0.33572588891577126 1105 88-89 0.2849260278562795 1105 90-91 0.2530846405335332 1105 92-93 0.23154557406207132 1105 94-95 0.24648084105575663 1105 96-97 0.23178011754446004 1105 98-99 0.24935813851847044 1105 100 0.21589737305917112 1106 1 0.11373699302885143 1106 2 0.1347605757303718 1106 3 0.15561861991514547 1106 4 0.17675843970985028 1106 5 0.1656299783483135 1106 6 0.20697606503640031 1106 7 0.2107204031427372 1106 8 0.1608195510346846 1106 9 0.24429065676031314 1106 10-11 0.26388002919043174 1106 12-13 0.24303475670163976 1106 14-15 0.27674640794700167 1106 16-17 0.29854891230417735 1106 18-19 0.2972978965552642 1106 20-21 0.3010634427964476 1106 22-23 0.3055088290627168 1106 24-25 0.2997711936081373 1106 26-27 0.28266750319032496 1106 28-29 0.2732701175777592 1106 30-31 0.32477395167796885 1106 32-33 0.3462297056897654 1106 34-35 0.33340209404682497 1106 36-37 0.33695227833484154 1106 38-39 0.32529569586989027 1106 40-41 0.39584019886775934 1106 42-43 0.3855429195809208 1106 44-45 0.32028455226658537 1106 46-47 0.34637774296743373 1106 48-49 0.3246304151537558 1106 50-51 0.35750640186807203 1106 52-53 0.36903513068590144 1106 54-55 0.3633437326246849 1106 56-57 0.29958108668640193 1106 58-59 0.3679387755183541 1106 60-61 0.33225566314216337 1106 62-63 0.3409286976478967 1106 64-65 0.36201025688286137 1106 66-67 0.3974513515869873 1106 68-69 0.38484524766730743 1106 70-71 0.386840279124236 1106 72-73 0.39313144733795014 1106 74-75 0.4355028166310362 1106 76-77 0.38993836785276415 1106 78-79 0.3360575703883093 1106 80-81 0.3823448446524651 1106 82-83 0.37087932537533774 1106 84-85 0.35703541498525837 1106 86-87 0.32152124374486135 1106 88-89 0.32550079884115846 1106 90-91 0.32026190710923785 1106 92-93 0.3131852183423476 1106 94-95 0.36245821517149324 1106 96-97 0.38304695059856897 1106 98-99 0.4161786360215025 1106 100 0.3778947373616326 1107 1 0.09802299819213545 1107 2 0.12142513744857197 1107 3 0.1664329760333736 1107 4 0.1605833822312519 1107 5 0.14078652142026726 1107 6 0.18175694346625448 1107 7 0.14442880475522912 1107 8 0.1440071847588058 1107 9 0.18774335745482063 1107 10-11 0.2025605848346288 1107 12-13 0.24813315922796875 1107 14-15 0.2663255340057873 1107 16-17 0.25467488669190885 1107 18-19 0.29003397951640153 1107 20-21 0.24872354823840936 1107 22-23 0.25487216137394597 1107 24-25 0.2699786339466499 1107 26-27 0.29101309460624236 1107 28-29 0.2849645289850642 1107 30-31 0.25372137262389316 1107 32-33 0.25357819403586745 1107 34-35 0.2562834714131341 1107 36-37 0.2431931985623308 1107 38-39 0.24238383269399577 1107 40-41 0.34220472232043875 1107 42-43 0.3003189836820255 1107 44-45 0.22655746190113746 1107 46-47 0.24734653236782123 1107 48-49 0.24711409985928867 1107 50-51 0.2755057563290251 1107 52-53 0.2678540316765776 1107 54-55 0.24842578549048255 1107 56-57 0.16990318238529767 1107 58-59 0.22170414879042966 1107 60-61 0.1959488583064939 1107 62-63 0.20473422983917544 1107 64-65 0.21010542889718664 1107 66-67 0.2135337278091498 1107 68-69 0.24429492805124653 1107 70-71 0.24126341761618875 1107 72-73 0.25159677493581256 1107 74-75 0.2272879710051896 1107 76-77 0.2518673354807248 1107 78-79 0.19818550354588993 1107 80-81 0.20426540686383987 1107 82-83 0.2259234827136929 1107 84-85 0.22368435507519635 1107 86-87 0.24908640468532184 1107 88-89 0.19971511817120202 1107 90-91 0.23270808828776168 1107 92-93 0.19229037031814755 1107 94-95 0.16515270728218212 1107 96-97 0.1968091522173907 1107 98-99 0.25937027058543194 1107 100 0.24980517937455105 1108 1 0.0714944460796687 1108 2 0.1315253719870917 1108 3 0.1450749505559621 1108 4 0.1094670813412506 1108 5 0.12798297289648985 1108 6 0.17603669547592204 1108 7 0.11074982327289007 1108 8 0.0918448229814075 1108 9 0.11277533734887157 1108 10-11 0.09090911561172277 1108 12-13 0.15280768638859143 1108 14-15 0.17677711326267342 1108 16-17 0.16483719984829293 1108 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-0.7644541526378958 2215 32-33 -0.6694605789219281 2215 34-35 -0.577086539005613 2215 36-37 -0.6059517240865588 2215 38-39 -0.6024832243645264 2215 40-41 -0.5882461786476583 2215 42-43 -0.5609912524314709 2215 44-45 -0.5156236306653312 2215 46-47 -0.4562089678065817 2215 48-49 -0.4464889545187205 2215 50-51 -0.4865917155153028 2215 52-53 -0.5043317999473089 2215 54-55 -0.5297704853768437 2215 56-57 -0.4826862848928073 2215 58-59 -0.4951045502740996 2215 60-61 -0.401412447130177 2215 62-63 -0.42845022001890953 2215 64-65 -0.4890430359802167 2215 66-67 -0.4102796586000821 2215 68-69 -0.4533296401864959 2215 70-71 -0.4493439550502245 2215 72-73 -0.40310336411364034 2215 74-75 -0.4026630465604413 2215 76-77 -0.3895395840230407 2215 78-79 -0.41437626064398003 2215 80-81 -0.45851470509141734 2215 82-83 -0.46513005054487166 2215 84-85 -0.4221086944461483 2215 86-87 -0.4310652495030922 2215 88-89 -0.3705512343391959 2215 90-91 -0.36347671997291897 2215 92-93 -0.3847889211190676 2215 94-95 -0.4121735380692435 2215 96-97 -0.4219065170747207 2215 98-99 -0.3935651457742928 2215 100 -0.3767621001699517 2216 1 -0.5521307390315648 2216 2 -0.6409360822384365 2216 3 -0.6598304711232679 2216 4 -0.755027739184051 2216 5 -0.6839638903000633 2216 6 -1.1586367053918138 2216 7 -1.0129101091927097 2216 8 -1.234491617724757 2216 9 -1.1962133469470402 2216 10-11 -0.9769595976260206 2216 12-13 -0.9815990646076784 2216 14-15 -1.1287819174616374 2216 16-17 -1.2149689845308131 2216 18-19 -1.1711239136359524 2216 20-21 -1.0689920892276916 2216 22-23 -1.0785532390269736 2216 24-25 -1.1480522868375758 2216 26-27 -1.1729670535958832 2216 28-29 -1.2770144922500108 2216 30-31 -1.2664151749276087 2216 32-33 -1.2997380329615567 2216 34-35 -1.5467960219272285 2216 36-37 -1.2712908373037308 2216 38-39 -1.216142013977958 2216 40-41 -1.3466443912861408 2216 42-43 -1.3020237283172946 2216 44-45 -1.2096751396301997 2216 46-47 -1.1239944381113887 2216 48-49 -1.2627449868093592 2216 50-51 -1.2791956109434324 2216 52-53 -1.4819914836773762 2216 54-55 -1.270881012578755 2216 56-57 -1.267622694183558 2216 58-59 -1.5331393107614488 2216 60-61 -1.6664025224898822 2216 62-63 -1.4569384055287742 2216 64-65 -1.4263868992942434 2216 66-67 -1.3475229950881626 2216 68-69 -1.3734058417000128 2216 70-71 -1.2742246577681513 2216 72-73 -1.4456728652009048 2216 74-75 -1.5996241124390203 2216 76-77 -1.7769581205159142 2216 78-79 -1.5299907601538756 2216 80-81 -1.4557731015332571 2216 82-83 -1.51491766378739 2216 84-85 -1.4648536407255932 2216 86-87 -1.4263877553617093 2216 88-89 -1.3847028369656442 2216 90-91 -1.4357687207073901 2216 92-93 -1.475306244697208 2216 94-95 -1.4045320690372272 2216 96-97 -1.495690891664374 2216 98-99 -1.4519317292407088 2216 100 -1.3518125134196595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 256955.0 3 41942.0 4 26643.0 5 21157.0 6 19129.0 7 19174.0 8 20985.0 9 23355.0 10 25711.0 11 28224.0 12 30714.0 13 32577.0 14 34065.0 15 35950.0 16 37731.0 17 39702.0 18 38350.0 19 36297.0 20 36420.0 21 38959.0 22 43569.0 23 50946.0 24 61182.0 25 74332.0 26 90113.0 27 106749.0 28 129691.0 29 159409.0 30 192173.0 31 233598.0 32 295771.0 33 388736.0 34 536812.0 35 788681.0 36 1322448.0 37 2254475.0 38 2523524.0 39 732612.0 40 1066.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91767526696531 18.918966360342637 13.592159908220122 23.57119846447193 2 16.108079644087322 19.72924770836054 38.975472914704596 25.187199732847542 3 18.51309703755538 24.96250686762253 29.609688157064895 26.9147079377572 4 13.248422242879936 16.727448494904024 37.41505858789514 32.6090706743209 5 14.109746174489121 36.88688556034429 34.27365185124371 14.729716413922878 6 31.668685564237464 36.53638813144958 17.840873316262975 13.95405298804998 7 28.272127571089477 31.686485820524236 21.731811415895425 18.30957519249086 8 26.351493619039594 34.9370880302137 19.962581950681095 18.748836400065606 9 25.618465570020792 16.97153747012951 20.543898772968742 36.86609818688096 10-11 24.708466839087066 26.407426544707167 28.066467279604108 20.81763933660166 12-13 25.12708330149084 23.75294669102258 28.08904717757898 23.030922829907606 14-15 22.612998593419313 25.750243318787923 25.129555306806083 26.507202780986677 16-17 22.702222752521674 28.98172453967342 25.584697860372536 22.73135484743237 18-19 22.746222523587655 28.203344094295673 27.108103921206023 21.94232946091065 20-21 23.521685462075975 27.36802578753799 26.876567207625733 22.233721542760296 22-23 23.361988464035342 27.181209804660295 26.815648288142658 22.64115344316171 24-25 23.06838329911437 27.316304706646218 26.763056966665456 22.85225502757396 26-27 22.963288057317712 27.397768812971744 26.735218651434394 22.903724478276146 28-29 22.941858099972826 27.359724536440073 26.883689187376497 22.814728176210608 30-31 23.0609690022669 27.60355903626719 26.620166880963524 22.715305080502386 32-33 22.871369513029098 27.47181566021888 26.82181309748145 22.83500172927057 34-35 23.038981260926295 27.199927118410926 26.898888846665013 22.862202773997765 36-37 23.201923964007126 27.318296931322845 26.70428168245636 22.775497422213668 38-39 23.033000367847762 27.473628418719176 26.71953589304764 22.773835320385423 40-41 23.03811403985513 27.255557145016834 26.877343345892747 22.82898546923529 42-43 22.923599606393797 27.369238625215225 26.779461904987716 22.927699863403262 44-45 22.95069075318169 27.439736765158695 26.739734197680665 22.869838283978954 46-47 23.0657685337124 27.345463737160074 26.699597818385467 22.88916991074206 48-49 23.015412310130586 27.32947107045475 26.711213785859734 22.943902833554926 50-51 22.949496731182627 27.355853776957613 26.71986291676354 22.974786575096225 52-53 22.99085402582305 27.310387830622286 26.67060876024866 23.028149383306005 54-55 23.007562833177207 27.456736398783367 26.687427167899124 22.8482736001403 56-57 22.877013956362546 27.326206268123798 26.732908589664987 23.063871185848665 58-59 22.89142391042321 27.09479603276574 26.828156896970377 23.18562315984067 60-61 23.01439762261343 27.295937484632848 26.68681528872702 23.002849604026707 62-63 22.88928772690223 27.395863290051594 26.72161432979791 22.993234653248273 64-65 22.999911383678516 27.158090270708318 26.74374547249793 23.098252873115236 66-67 22.81106791933838 27.177916985756568 26.773713102837487 23.23730199206756 68-69 22.992136797349406 27.249696545272922 26.70863354614606 23.04953311123161 70-71 22.983724553582128 27.060849927408594 26.776284408878652 23.179141110130626 72-73 22.796897110245975 27.21895092300017 26.856445633395833 23.127706333358024 74-75 22.80716474420464 27.17298593332521 26.79888958313929 23.22095973933086 76-77 22.883053577683526 27.165059942889897 26.677631193149743 23.274255286276833 78-79 22.910239634052836 27.18280924547963 26.800529180149862 23.106421940317674 80-81 22.74740330046695 27.10997580632835 26.89892246660839 23.243698426596314 82-83 22.76315439516619 27.08409906693475 26.94812324927903 23.204623288620034 84-85 22.77076749481007 27.039825806510898 26.822296349185248 23.36711034949379 86-87 22.850987027782622 27.147059714460497 26.789770544392795 23.212182713364086 88-89 22.725554148630792 27.052945243742098 26.89589217474726 23.325608432879854 90-91 22.678276611171402 26.984199766330864 26.915134292335402 23.422389330162332 92-93 22.759681253266795 27.13242451051192 26.802222066257503 23.30567216996378 94-95 22.754389403002772 26.890119655075985 26.925342746351998 23.43014819556924 96-97 22.754920531582474 26.927240427602307 26.904075750387555 23.41376329042766 98-99 22.68520091766603 27.013350555885324 26.87075476959912 23.43069375684953 100 22.793942538571546 26.792614305049177 26.866062368431333 23.547380787947944 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3560.0 1 2805.5 2 2793.0 3 3429.0 4 3004.0 5 2504.5 6 2198.0 7 2137.0 8 2278.5 9 2328.5 10 2126.0 11 1905.5 12 1948.5 13 2073.0 14 2261.0 15 2432.5 16 2661.0 17 3166.0 18 3883.5 19 4917.5 20 6534.5 21 8917.0 22 12521.5 23 18306.0 24 26571.0 25 37507.5 26 51904.0 27 69772.0 28 90617.5 29 113898.5 30 135739.0 31 156808.0 32 178330.5 33 197063.5 34 214788.5 35 234087.5 36 256159.5 37 280193.0 38 303351.5 39 321091.5 40 337202.0 41 353793.5 42 363395.5 43 367880.5 44 371603.5 45 375896.5 46 380058.5 47 375788.0 48 368744.5 49 368468.0 50 368524.5 51 364490.5 52 354727.5 53 347249.0 54 341181.0 55 326983.0 56 308314.0 57 283972.5 58 258565.0 59 238288.5 60 215709.0 61 188102.5 62 160690.5 63 135436.5 64 111421.5 65 91060.0 66 72811.5 67 56744.0 68 43857.0 69 33133.5 70 24935.0 71 18659.5 72 14488.0 73 11083.0 74 8305.0 75 6200.0 76 4248.0 77 2910.5 78 2141.5 79 1471.0 80 990.5 81 702.0 82 518.0 83 427.0 84 355.0 85 289.5 86 228.5 87 178.5 88 166.5 89 144.0 90 110.5 91 86.5 92 68.0 93 54.0 94 43.0 95 35.5 96 30.5 97 26.0 98 28.0 99 30.0 100 51.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053259823450333504 2 0.015263260777288713 3 0.017950259498517394 4 0.014598436351417698 5 0.013887443562638973 6 0.013019478339974038 7 0.020960436760100044 8 0.013508862986795754 9 0.013989013961035935 10-11 0.016085057637045937 12-13 0.01746549168798645 14-15 0.014219855775574481 16-17 0.0153602143393949 18-19 0.013245703318221811 20-21 0.016168160690279813 22-23 0.01653289075725072 24-25 0.017234649873447902 26-27 0.010729527539751652 28-29 0.015092437834530186 30-31 0.013758172146497388 32-33 0.015346363830522586 34-35 0.012959459468194016 36-37 0.013388825243235711 38-39 0.019187571624444004 40-41 0.015327896485359505 42-43 0.01235003707781225 44-45 0.020138639900342818 46-47 0.018310372729197527 48-49 0.018388858946140632 50-51 0.01356888185857578 52-53 0.013864359381185118 54-55 0.013956696107000536 56-57 0.019773909833371916 58-59 0.00804714565481374 60-61 0.011352800439005729 62-63 0.016666779009683073 64-65 0.021703747402914166 66-67 0.014815427657083931 68-69 0.015748028587819658 70-71 0.023485846211151747 72-73 0.0192522073325148 74-75 0.01940456293011024 76-77 0.012650131436712361 78-79 0.013651984911809655 80-81 0.01934916089462099 82-83 0.014136752722340603 84-85 0.01392899508925591 86-87 0.017202332019412504 88-89 0.016066590291882853 90-91 0.0165836759564492 92-93 0.011699063160813549 94-95 0.01664831166451999 96-97 0.008675035390358587 98-99 0.006602075895802436 100 0.004672238326260186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0829927E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.38942569002391 #Duplication Level Percentage of deduplicated Percentage of total 1 73.32913739533312 38.41671394486359 2 14.560788089373972 15.25662651192886 3 4.822817217752887 7.579938727380984 4 2.198963186222345 4.608096737587751 5 1.2780101538520359 3.3477108993163633 6 0.8409552166751726 2.6434296499585153 7 0.5528780604340957 2.0275474843929624 8 0.3839088018316976 1.6090209316246273 9 0.28538004695747343 1.345580708714468 >10 1.5980231262124889 15.052740304047147 >50 0.10223466799365688 3.6822417461279575 >100 0.04580391166024012 3.974849329699458 >500 9.762493904284974E-4 0.32975896911623304 >1k 1.2387631034689904E-4 0.12574405524102986 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.846734516308374E-5 0.0 4 0.0 9.23367258154187E-6 0.0 1.846734516308374E-5 0.0 5 0.0 9.23367258154187E-6 0.0 1.846734516308374E-5 0.0 6 0.0 9.23367258154187E-6 0.0 1.846734516308374E-5 0.0 7 0.0 9.23367258154187E-6 0.0 1.846734516308374E-5 0.0 8 0.0 9.23367258154187E-6 0.0 1.846734516308374E-5 0.0 9 9.23367258154187E-6 1.846734516308374E-5 0.0 1.846734516308374E-5 0.0 10-11 9.23367258154187E-6 1.846734516308374E-5 0.0 1.846734516308374E-5 0.0 12-13 9.23367258154187E-6 2.3084181453854676E-5 0.0 2.3084181453854676E-5 0.0 14-15 9.23367258154187E-6 3.231785403539654E-5 0.0 3.693469032616748E-5 0.0 16-17 1.846734516308374E-5 3.693469032616748E-5 0.0 3.693469032616748E-5 0.0 18-19 1.846734516308374E-5 3.693469032616748E-5 0.0 9.695356210618963E-5 0.0 20-21 1.846734516308374E-5 3.693469032616748E-5 0.0 1.708229427585246E-4 0.0 22-23 1.846734516308374E-5 3.693469032616748E-5 0.0 3.1856170406319455E-4 0.0 24-25 1.846734516308374E-5 3.693469032616748E-5 0.0 7.017591161971821E-4 0.0 26-27 1.846734516308374E-5 3.693469032616748E-5 0.0 0.0012465457985081523 0.0 28-29 1.846734516308374E-5 4.155152661693842E-5 0.0 0.0025069421058886176 0.0 30-31 1.846734516308374E-5 5.540203548925122E-5 0.0 0.0064174024441716 0.0 32-33 1.846734516308374E-5 5.540203548925122E-5 0.0 0.01446454809898534 4.616836290770935E-6 34-35 2.770101774462561E-5 6.463570807079309E-5 0.0 0.02813038351966731 9.23367258154187E-6 36-37 2.770101774462561E-5 1.061872346877315E-4 0.0 0.05049895534845249 9.23367258154187E-6 38-39 2.770101774462561E-5 1.4773876130466993E-4 9.23367258154187E-6 0.0844881041211081 9.23367258154187E-6 40-41 2.770101774462561E-5 1.569724338862118E-4 1.846734516308374E-5 0.12284478002483304 9.23367258154187E-6 42-43 2.770101774462561E-5 2.0314079679392114E-4 1.846734516308374E-5 0.16325133124166025 9.23367258154187E-6 44-45 2.770101774462561E-5 2.0314079679392114E-4 1.846734516308374E-5 0.21157575669715964 9.23367258154187E-6 46-47 2.770101774462561E-5 2.216081419570049E-4 1.846734516308374E-5 0.2721624993409466 9.23367258154187E-6 48-49 2.770101774462561E-5 2.216081419570049E-4 1.846734516308374E-5 0.33770772416102157 9.23367258154187E-6 50-51 2.770101774462561E-5 2.5854283228317235E-4 1.846734516308374E-5 0.40783285058154134 9.23367258154187E-6 52-53 2.770101774462561E-5 2.677765048647142E-4 1.846734516308374E-5 0.4809173690644452 9.23367258154187E-6 54-55 2.770101774462561E-5 2.8162701373702704E-4 1.846734516308374E-5 0.5607794032222009 9.23367258154187E-6 56-57 4.616836290770935E-5 2.9547752260933985E-4 1.846734516308374E-5 0.6557431088870682 9.23367258154187E-6 58-59 4.616836290770935E-5 3.0471119519088173E-4 1.846734516308374E-5 0.7613763232199071 9.23367258154187E-6 60-61 4.616836290770935E-5 3.0471119519088173E-4 1.846734516308374E-5 0.8742579705292566 9.23367258154187E-6 62-63 6.463570807079309E-5 3.139448677724236E-4 1.846734516308374E-5 0.9900251405203377 9.23367258154187E-6 64-65 6.463570807079309E-5 3.231785403539655E-4 1.846734516308374E-5 1.113026892979057 9.23367258154187E-6 66-67 6.463570807079309E-5 3.324122129355073E-4 1.846734516308374E-5 1.254819169141214 9.23367258154187E-6 68-69 6.463570807079309E-5 3.3702904922627825E-4 1.846734516308374E-5 1.41103905871203 9.23367258154187E-6 70-71 6.463570807079309E-5 3.5087955809859106E-4 1.846734516308374E-5 1.5797151725953462 9.23367258154187E-6 72-73 6.463570807079309E-5 3.785805758432167E-4 1.846734516308374E-5 1.7570894060504747 9.23367258154187E-6 74-75 7.386938065233496E-5 4.0628159358784227E-4 1.846734516308374E-5 1.9403685731214995 9.23367258154187E-6 76-77 7.386938065233496E-5 4.0628159358784227E-4 1.846734516308374E-5 2.137073500126086 9.23367258154187E-6 78-79 7.386938065233496E-5 4.1551526616938415E-4 1.846734516308374E-5 2.3559992601981525 9.23367258154187E-6 80-81 7.386938065233496E-5 4.24748938750926E-4 1.846734516308374E-5 2.591661051824264 9.23367258154187E-6 82-83 7.386938065233496E-5 4.24748938750926E-4 1.846734516308374E-5 2.8384309515659707 9.23367258154187E-6 84-85 7.386938065233496E-5 4.339826113324679E-4 1.846734516308374E-5 3.0870937541868932 9.23367258154187E-6 86-87 7.386938065233496E-5 4.3859944762323884E-4 1.846734516308374E-5 3.3471832266274744 9.23367258154187E-6 88 7.386938065233496E-5 4.432162839140098E-4 1.846734516308374E-5 3.5623878166491796 9.23367258154187E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10030 0.0 36.84691 1 GTATCAA 16210 0.0 33.35756 1 TCAACGC 16860 0.0 30.804604 4 ATCAACG 16935 0.0 30.474464 3 CAACGCA 18210 0.0 28.54724 5 TACCTGG 9470 0.0 27.695114 2 AACGCAG 19210 0.0 27.378878 6 GTACCTG 10135 0.0 26.90814 1 TATCAAC 19535 0.0 26.490664 2 TATAACG 1170 0.0 25.71065 2 GTACATG 33735 0.0 23.638754 1 ACGCAGA 22720 0.0 23.10765 7 TACATGG 34530 0.0 22.173971 2 CGCAGAG 24075 0.0 21.707775 8 ATAACGC 1605 0.0 21.085135 3 ACATGGG 35275 0.0 20.812918 3 ACCTGGG 12485 0.0 20.479969 3 TAACGCA 1525 0.0 20.341595 4 TATCACG 1705 0.0 17.643085 2 GCAGAGT 29130 0.0 17.456999 9 >>END_MODULE