FastQCFastQC Report
Wed 25 May 2016
SRR2049432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10829927
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258170.23838572503766647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229810.2121990295964137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135040.12469151454114141No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117220.10823711000083379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154100.049.6655161
GTATCAA266500.037.6145861
TATAACG11800.034.2547952
ATCAACG314750.031.0152823
TCAACGC316350.030.9475574
CAACGCA332250.029.5514225
TAACGCA15050.029.355964
TATCAAC337400.029.0724952
AACGCAG347050.028.3932086
ATAACGC16500.026.7761943
TACCTGG88650.025.5018252
GTACCTG94350.024.7291851
ACGCAGA401250.024.55787
CGCAGAG415750.023.7352288
GTACATG331150.021.59181
GTGGTAT77450.020.8933011
GCAGAGT475600.020.6890539
TATACCG8750.020.411765
TACATGG339500.020.2400952
GTATAAC33150.019.8661881
Produced by FastQC (version 0.11.5)