##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10829927 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.748201165160207 31.0 31.0 34.0 30.0 34.0 2 31.81620393193786 31.0 31.0 34.0 30.0 34.0 3 31.86290276933538 33.0 31.0 34.0 30.0 34.0 4 35.627183174918905 37.0 35.0 37.0 33.0 37.0 5 35.71176804792867 37.0 35.0 37.0 33.0 37.0 6 35.79291254687128 37.0 35.0 37.0 35.0 37.0 7 35.786951195515904 37.0 35.0 37.0 35.0 37.0 8 35.80643803046872 37.0 35.0 37.0 35.0 37.0 9 37.512556917512 39.0 37.0 39.0 35.0 39.0 10-11 37.48506190300267 39.0 37.0 39.0 34.5 39.0 12-13 37.46103717965966 39.0 37.0 39.0 34.5 39.0 14-15 38.763897716023386 40.0 38.0 41.0 35.0 41.0 16-17 38.78000567316843 40.0 38.0 41.0 35.0 41.0 18-19 38.82582823503797 40.0 38.0 41.0 35.0 41.0 20-21 38.836884126735114 40.0 38.0 41.0 35.0 41.0 22-23 38.80725234805368 40.0 38.0 41.0 35.0 41.0 24-25 38.78641651970507 40.0 38.0 41.0 35.0 41.0 26-27 38.6866984883647 40.0 38.0 41.0 34.5 41.0 28-29 38.607683782171385 40.0 38.0 41.0 34.0 41.0 30-31 38.52067580880278 40.0 38.0 41.0 34.0 41.0 32-33 38.4162893249419 40.0 38.0 41.0 34.0 41.0 34-35 38.32034560343759 40.0 38.0 41.0 34.0 41.0 36-37 38.21952313251973 40.0 38.0 41.0 34.0 41.0 38-39 38.13850956705433 40.0 38.0 41.0 33.5 41.0 40-41 38.05950580276303 40.0 38.0 41.0 33.0 41.0 42-43 37.93716596612332 40.0 37.0 41.0 33.0 41.0 44-45 37.78617778310048 40.0 37.0 41.0 33.0 41.0 46-47 37.58921509812578 40.0 37.0 41.0 33.0 41.0 48-49 37.389315874428334 40.0 37.0 41.0 32.5 41.0 50-51 37.23361034658868 40.0 36.5 41.0 32.0 41.0 52-53 37.09829364500795 40.0 36.0 41.0 32.0 41.0 54-55 36.935587054280234 39.0 36.0 41.0 31.5 41.0 56-57 36.76123869533008 39.0 35.0 41.0 31.0 41.0 58-59 36.551356024837474 39.0 35.0 41.0 31.0 41.0 60-61 36.33407695176523 39.0 35.0 41.0 31.0 41.0 62-63 36.090179324385105 38.0 35.0 40.0 31.0 41.0 64-65 35.84151896868741 37.5 35.0 40.0 30.5 41.0 66-67 35.55173866822925 37.0 35.0 40.0 30.0 41.0 68-69 35.2395697588728 36.5 34.5 39.5 30.0 41.0 70-71 34.928872650757484 36.0 34.0 39.0 29.5 41.0 72-73 34.60004107137564 36.0 34.0 39.0 29.0 41.0 74-75 34.28348658305822 35.0 34.0 38.5 29.0 40.0 76-77 33.97346990427544 35.0 34.0 37.0 29.0 39.5 78-79 33.676318870847425 35.0 34.0 37.0 29.0 39.0 80-81 33.392008967373464 35.0 34.0 36.0 29.0 39.0 82-83 33.1404480842761 35.0 34.0 36.0 29.0 37.5 84-85 32.89488165525031 35.0 33.0 36.0 29.0 37.0 86-87 32.682054228066356 35.0 33.0 35.0 28.5 37.0 88-89 32.49059088763941 35.0 33.0 35.0 28.0 36.0 90-91 32.321042607212405 35.0 33.0 35.0 27.0 36.0 92-93 32.16803446597562 35.0 33.0 35.0 27.0 36.0 94-95 32.0217718457382 35.0 33.0 35.0 27.0 36.0 96-97 31.866120658061686 35.0 33.0 35.0 27.0 35.0 98-99 31.727643593534843 35.0 33.0 35.0 27.0 35.0 100 31.616502955190743 35.0 33.0 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1948818606374978 1101 2 0.21102743402582647 1101 3 0.37818917282919173 1101 4 0.2538043308305262 1101 5 0.19958397301230946 1101 6 0.16713216667169917 1101 7 0.13115824355627126 1101 8 0.16216942459998762 1101 9 0.24216550380504742 1101 10-11 0.26451600233513517 1101 12-13 0.19636591191745367 1101 14-15 0.31631059017446717 1101 16-17 0.3140381585259533 1101 18-19 0.30348616057634104 1101 20-21 0.31694954753393745 1101 22-23 0.32914367079533946 1101 24-25 0.2976262275998067 1101 26-27 0.3018261995739948 1101 28-29 0.3291079058681987 1101 30-31 0.32438844921978216 1101 32-33 0.32389911153080675 1101 34-35 0.33196193949554953 1101 36-37 0.35295104677384614 1101 38-39 0.32528080902916656 1101 40-41 0.33242018999526124 1101 42-43 0.33362661579460706 1101 44-45 0.34364517877197187 1101 46-47 0.32740231214972937 1101 48-49 0.35682875729659 1101 50-51 0.35821101503741204 1101 52-53 0.3731640728535126 1101 54-55 0.3456542109493981 1101 56-57 0.3528499671307159 1101 58-59 0.30914132895708946 1101 60-61 0.3108701184794711 1101 62-63 0.29483698729055163 1101 64-65 0.295558740238107 1101 66-67 0.3143468815524102 1101 68-69 0.28888258519503296 1101 70-71 0.27716083983154505 1101 72-73 0.29734388924547517 1101 74-75 0.31047588767206946 1101 76-77 0.2757386275566631 1101 78-79 0.26590214068433937 1101 80-81 0.2988524308391476 1101 82-83 0.25679214796852534 1101 84-85 0.3036110452657894 1101 86-87 0.22413084449095777 1101 88-89 0.3045420523535043 1101 90-91 0.3001592881784916 1101 92-93 0.3314513552376681 1101 94-95 0.3496682142038239 1101 96-97 0.287612681283953 1101 98-99 0.3270140917735773 1101 100 0.31098196515212706 1102 1 0.18462934494912275 1102 2 0.15054054580293297 1102 3 0.27690492242384224 1102 4 0.22012349762663774 1102 5 0.21506876173278755 1102 6 0.1748218305784519 1102 7 0.17863472907230715 1102 8 0.16621729408966246 1102 9 0.14418189737847342 1102 10-11 0.19623075634426357 1102 12-13 0.21270509837187035 1102 14-15 0.25504133334268175 1102 16-17 0.23270304418186782 1102 18-19 0.26757835298379007 1102 20-21 0.2491820631724906 1102 22-23 0.21916717605885339 1102 24-25 0.2611324944666791 1102 26-27 0.2631111718512429 1102 28-29 0.26548736397355555 1102 30-31 0.26083496267440154 1102 32-33 0.2876685353950492 1102 34-35 0.27963180541423327 1102 36-37 0.2815409186598714 1102 38-39 0.2837791412273063 1102 40-41 0.3222328644902248 1102 42-43 0.31614108150513687 1102 44-45 0.3273056466822908 1102 46-47 0.31374498981539034 1102 48-49 0.2919773639592691 1102 50-51 0.2812258237930578 1102 52-53 0.2629714338385085 1102 54-55 0.25153760771403455 1102 56-57 0.32415974046651996 1102 58-59 0.3188552711364139 1102 60-61 0.278090238393915 1102 62-63 0.28887542595336413 1102 64-65 0.2269337937910194 1102 66-67 0.19493915953199092 1102 68-69 0.23290514128509443 1102 70-71 0.25633577127321416 1102 72-73 0.28809144180320345 1102 74-75 0.257279127955492 1102 76-77 0.2545597022489119 1102 78-79 0.24796001166998138 1102 80-81 0.3070540121887788 1102 82-83 0.30441433350218006 1102 84-85 0.21982463081485548 1102 86-87 0.24158691969654456 1102 88-89 0.21379754897323977 1102 90-91 0.28740348167356444 1102 92-93 0.3142805787618457 1102 94-95 0.22974426694488415 1102 96-97 0.24235030999563634 1102 98-99 0.26377790894063224 1102 100 0.2386724961818949 1103 1 0.1268882015125179 1103 2 0.11431764659843324 1103 3 0.25463008421570876 1103 4 0.15945251632363977 1103 5 0.1289620469107291 1103 6 0.13267091963433586 1103 7 0.1344603759185219 1103 8 0.11859481460809462 1103 9 0.19402285430548716 1103 10-11 0.22273665033411305 1103 12-13 0.1951730272256711 1103 14-15 0.2662323844612686 1103 16-17 0.22905498677537395 1103 18-19 0.24096828599309816 1103 20-21 0.25070548732577436 1103 22-23 0.24432987424180652 1103 24-25 0.2674234674139342 1103 26-27 0.2773322290828233 1103 28-29 0.2650309536984423 1103 30-31 0.2612300483495176 1103 32-33 0.23899880238999316 1103 34-35 0.24418524819279241 1103 36-37 0.2281578389251706 1103 38-39 0.2339790015128358 1103 40-41 0.23888581429053346 1103 42-43 0.25022673474430945 1103 44-45 0.2862160141332666 1103 46-47 0.2583380706883105 1103 48-49 0.28360324997616004 1103 50-51 0.26597949391160114 1103 52-53 0.2519572854327876 1103 54-55 0.2521087770412791 1103 56-57 0.2102552567938858 1103 58-59 0.20313738288374594 1103 60-61 0.15957498402983106 1103 62-63 0.17477461877354017 1103 64-65 0.17556301868634705 1103 66-67 0.14709513646963757 1103 68-69 0.1592830440702926 1103 70-71 0.14906699250930444 1103 72-73 0.16381890870362525 1103 74-75 0.12833466419977668 1103 76-77 0.16219562066495996 1103 78-79 0.14569721274913405 1103 80-81 0.11936400810557757 1103 82-83 0.13594573596554937 1103 84-85 0.15378251675340948 1103 86-87 0.11276694288262945 1103 88-89 0.11472860947093722 1103 90-91 0.13417231547917652 1103 92-93 0.17632413792630075 1103 94-95 0.14140635248276823 1103 96-97 0.1455349914064854 1103 98-99 0.18321245484632698 1103 100 0.16271670972237118 1104 1 0.12948609769814823 1104 2 0.10756380823239908 1104 3 0.29186275113369575 1104 4 0.1679737501850127 1104 5 0.14856161462834905 1104 6 0.14816648745023997 1104 7 0.1739486068051761 1104 8 0.1435839228511 1104 9 0.1820722467468059 1104 10-11 0.16613443926634375 1104 12-13 0.1691076385161594 1104 14-15 0.2693340008314422 1104 16-17 0.25850778954157505 1104 18-19 0.22356611967804696 1104 20-21 0.24169526303162314 1104 22-23 0.20617388874772047 1104 24-25 0.22585772605205534 1104 26-27 0.21198744675162118 1104 28-29 0.21417093751608718 1104 30-31 0.231404560428615 1104 32-33 0.2987837536528417 1104 34-35 0.2597673679426933 1104 36-37 0.24303019668188597 1104 38-39 0.25713000986371526 1104 40-41 0.25244328310299124 1104 42-43 0.27353330587180835 1104 44-45 0.2926226699273613 1104 46-47 0.2829985857468458 1104 48-49 0.2401202286129731 1104 50-51 0.25801425804763056 1104 52-53 0.27693244816070717 1104 54-55 0.2465295227135229 1104 56-57 0.2727462971379637 1104 58-59 0.30947225699794956 1104 60-61 0.2680929368037539 1104 62-63 0.27439175323401344 1104 64-65 0.24395185441524347 1104 66-67 0.2940976123306527 1104 68-69 0.2749288763994997 1104 70-71 0.29501392351878053 1104 72-73 0.335814275012396 1104 74-75 0.315796774151913 1104 76-77 0.29291061246455286 1104 78-79 0.2930052728859849 1104 80-81 0.26120135822772284 1104 82-83 0.2800472448933107 1104 84-85 0.27494080232831664 1104 86-87 0.21502706813066652 1104 88-89 0.2341554831090562 1104 90-91 0.2373202502575822 1104 92-93 0.2968262496910441 1104 94-95 0.2721705288274947 1104 96-97 0.29433703830631686 1104 98-99 0.3128300195847089 1104 100 0.29592748214991715 1105 1 0.16428756098417097 1105 2 0.14451554002788214 1105 3 0.22039764518671134 1105 4 0.15526641096563054 1105 5 0.14104821736658124 1105 6 0.11740794389452702 1105 7 0.12508971815323378 1105 8 0.11372671766476117 1105 9 0.18991652258367253 1105 10-11 0.17540122954508774 1105 12-13 0.17678372447910817 1105 14-15 0.22585637993379493 1105 16-17 0.2503684613740731 1105 18-19 0.25232791671042065 1105 20-21 0.21117495685350463 1105 22-23 0.2115023787921544 1105 24-25 0.23433212634283507 1105 26-27 0.2347615984953748 1105 28-29 0.2323306689728497 1105 30-31 0.26378191460364064 1105 32-33 0.23637817949101958 1105 34-35 0.25607182839952713 1105 36-37 0.2506524622664159 1105 38-39 0.24614746366280116 1105 40-41 0.26436456268599073 1105 42-43 0.2683596015964227 1105 44-45 0.23042616510946345 1105 46-47 0.2543568766847457 1105 48-49 0.2460393227321518 1105 50-51 0.21128353218782792 1105 52-53 0.182282217031009 1105 54-55 0.16132664842206879 1105 56-57 0.19181873948550532 1105 58-59 0.21188715783963374 1105 60-61 0.171282715757485 1105 62-63 0.18484434016640705 1105 64-65 0.15956509796974672 1105 66-67 0.17602905852309192 1105 68-69 0.20958957409170154 1105 70-71 0.18726475201711423 1105 72-73 0.2069156142921571 1105 74-75 0.16720941431278646 1105 76-77 0.15984736574468172 1105 78-79 0.16430461054249434 1105 80-81 0.13836952842245864 1105 82-83 0.13060411530412352 1105 84-85 0.1238248187208768 1105 86-87 0.13677381810986589 1105 88-89 0.16242894565079524 1105 90-91 0.17174502274215797 1105 92-93 0.22730240771897314 1105 94-95 0.1862390579464872 1105 96-97 0.20060052985619592 1105 98-99 0.2602223409524953 1105 100 0.21774539473537757 1106 1 0.09939980775395085 1106 2 0.08097615517471723 1106 3 0.1779696379472604 1106 4 0.11600744092937276 1106 5 0.12080374284616369 1106 6 0.10812391413409728 1106 7 0.10561903991346355 1106 8 0.11944753280820919 1106 9 0.14817970128957114 1106 10-11 0.13545582383493127 1106 12-13 0.1382132677776724 1106 14-15 0.21850226218774083 1106 16-17 0.1689778678396081 1106 18-19 0.15998824586965554 1106 20-21 0.1935196786857034 1106 22-23 0.19664963955735004 1106 24-25 0.17359272157993644 1106 26-27 0.17065879265419426 1106 28-29 0.20972409725745678 1106 30-31 0.2159149547159842 1106 32-33 0.17264964543061723 1106 34-35 0.15744382578687777 1106 36-37 0.18001467981652297 1106 38-39 0.17360095052282531 1106 40-41 0.1909721990888471 1106 42-43 0.21648582596629495 1106 44-45 0.1925578943930617 1106 46-47 0.20511029957891935 1106 48-49 0.22430274449080656 1106 50-51 0.15081960959874863 1106 52-53 0.15727287147339553 1106 54-55 0.17189852023066265 1106 56-57 0.17204225715853738 1106 58-59 0.1630707740455719 1106 60-61 0.1573486719357362 1106 62-63 0.14750502252799436 1106 64-65 0.1576948096021198 1106 66-67 0.1592928860783971 1106 68-69 0.1992671745292398 1106 70-71 0.19629436343014106 1106 72-73 0.21415340167621366 1106 74-75 0.17739535593979383 1106 76-77 0.150293486248529 1106 78-79 0.1685114729543642 1106 80-81 0.17046294616060464 1106 82-83 0.15672295231740918 1106 84-85 0.14874288148057957 1106 86-87 0.10588503340491684 1106 88-89 0.10754879837543996 1106 90-91 0.12831099678381008 1106 92-93 0.13724570391320867 1106 94-95 0.1292230770933216 1106 96-97 0.1488829107744607 1106 98-99 0.17327755545957757 1106 100 0.16483776271387995 1107 1 0.08320788633431064 1107 2 0.06032872771940845 1107 3 0.14142232353097128 1107 4 0.11123588087222203 1107 5 0.1044331032623873 1107 6 0.07432462988992938 1107 7 0.055478545459777706 1107 8 0.10233417195885153 1107 9 0.14192974429298744 1107 10-11 0.09981231836455606 1107 12-13 0.0966808707972433 1107 14-15 0.13623954704393526 1107 16-17 0.11758269140500488 1107 18-19 0.13961524989671403 1107 20-21 0.16305871756487988 1107 22-23 0.1348098501953814 1107 24-25 0.11351576787402706 1107 26-27 0.16655394326107142 1107 28-29 0.178007363024264 1107 30-31 0.1819476490636589 1107 32-33 0.18140804397032895 1107 34-35 0.1720889739180791 1107 36-37 0.14831370555606327 1107 38-39 0.15708096163263008 1107 40-41 0.22276416087631645 1107 42-43 0.15475224312292113 1107 44-45 0.165869831133584 1107 46-47 0.18085390988740357 1107 48-49 0.1748021482085491 1107 50-51 0.14868951559741816 1107 52-53 0.13328988264932917 1107 54-55 0.17031929724272743 1107 56-57 0.1540132866389783 1107 58-59 0.1875406587365589 1107 60-61 0.16579145107272097 1107 62-63 0.16161356504351687 1107 64-65 0.14062021601172603 1107 66-67 0.1549435370477923 1107 68-69 0.11187906648459034 1107 70-71 0.14165104084439406 1107 72-73 0.14906828843422204 1107 74-75 0.1601926451775384 1107 76-77 0.1583549451981625 1107 78-79 0.13373240767325 1107 80-81 0.12363928595890172 1107 82-83 0.14705165451472624 1107 84-85 0.13099630495230485 1107 86-87 0.1398935856603032 1107 88-89 0.1249031489032646 1107 90-91 0.15145209608009225 1107 92-93 0.19502324935638882 1107 94-95 0.18779518813407492 1107 96-97 0.14039860445775076 1107 98-99 0.18314874121058367 1107 100 0.16091391094104068 1108 1 0.035395137441017965 1108 2 0.06358061988725794 1108 3 0.14915061945699293 1108 4 0.08161637794107435 1108 5 0.048292920192686495 1108 6 0.07243479179791024 1108 7 0.0739417248805907 1108 8 0.05458228615803762 1108 9 0.08751184492923159 1108 10-11 0.07814554943940522 1108 12-13 0.07857070020693868 1108 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2215 1 -0.30732756122708693 2215 2 -0.3200592141417893 2215 3 -0.25982833563470464 2215 4 -0.2660914977549922 2215 5 -0.26674918462985175 2215 6 -0.21352014273406894 2215 7 -0.26178631721818135 2215 8 -0.2918758514838089 2215 9 -0.3835326618966306 2215 10-11 -0.3798112708389354 2215 12-13 -0.3852876268783021 2215 14-15 -0.5236484668485062 2215 16-17 -0.5545868577025956 2215 18-19 -0.48998227000800654 2215 20-21 -0.4950489259933377 2215 22-23 -0.4562007728510338 2215 24-25 -0.45072202880260903 2215 26-27 -0.45208458218771597 2215 28-29 -0.4233015724920932 2215 30-31 -0.4477087893154277 2215 32-33 -0.4340133758730005 2215 34-35 -0.417797583263372 2215 36-37 -0.4512485150822272 2215 38-39 -0.46490745849072823 2215 40-41 -0.48875135571957173 2215 42-43 -0.48713007865287494 2215 44-45 -0.4918546280802687 2215 46-47 -0.49489153638104 2215 48-49 -0.46103348136524147 2215 50-51 -0.4480639742789023 2215 52-53 -0.4412140609790214 2215 54-55 -0.3811249925411815 2215 56-57 -0.398462613746986 2215 58-59 -0.38876768212315227 2215 60-61 -0.4016705712666919 2215 62-63 -0.42276496193950663 2215 64-65 -0.4788761411955349 2215 66-67 -0.43973866222610525 2215 68-69 -0.4547062361665013 2215 70-71 -0.43144068226564514 2215 72-73 -0.43038376869932904 2215 74-75 -0.3992701081280572 2215 76-77 -0.3536214856716313 2215 78-79 -0.3939688016526688 2215 80-81 -0.41751355759552666 2215 82-83 -0.4119825010067899 2215 84-85 -0.415409479561589 2215 86-87 -0.3657553722283282 2215 88-89 -0.39536961561431383 2215 90-91 -0.40979268231379606 2215 92-93 -0.4041246894278885 2215 94-95 -0.3810860165574752 2215 96-97 -0.39760678149642814 2215 98-99 -0.37460992856018294 2215 100 -0.35372768923857123 2216 1 -0.24768382393937216 2216 2 -0.22439363665195344 2216 3 -0.25198751564092703 2216 4 -0.19166119063328324 2216 5 -0.30763036628046336 2216 6 -0.25708544512366416 2216 7 -0.38699207029487326 2216 8 -0.27881866997267934 2216 9 -0.4384332493111103 2216 10-11 -0.33790180055810026 2216 12-13 -0.3623517777045038 2216 14-15 -0.5631090600603059 2216 16-17 -0.5701965079509748 2216 18-19 -0.5041156879037416 2216 20-21 -0.47812125736027156 2216 22-23 -0.49876298642450223 2216 24-25 -0.5204803584990003 2216 26-27 -0.535275161979591 2216 28-29 -0.5720896521354959 2216 30-31 -0.6019770112328899 2216 32-33 -0.6070660159689822 2216 34-35 -0.5978867606379197 2216 36-37 -0.6412926406377792 2216 38-39 -0.5867582990203175 2216 40-41 -0.6445319070206708 2216 42-43 -0.7095726516819383 2216 44-45 -0.7554143756135474 2216 46-47 -0.7066327699786825 2216 48-49 -0.7156635045054784 2216 50-51 -0.6706928216094354 2216 52-53 -0.6696098517110443 2216 54-55 -0.6281178374285545 2216 56-57 -0.6431210023428306 2216 58-59 -0.6365285764795416 2216 60-61 -0.6066896522934968 2216 62-63 -0.6122942621038945 2216 64-65 -0.6730219110866358 2216 66-67 -0.5929016339189488 2216 68-69 -0.6186554774750164 2216 70-71 -0.6636857786542549 2216 72-73 -0.6328097888559086 2216 74-75 -0.5986483129314522 2216 76-77 -0.5367181586968286 2216 78-79 -0.576749387279996 2216 80-81 -0.6741146148435107 2216 82-83 -0.6323003891697425 2216 84-85 -0.7770151654024531 2216 86-87 -0.6652635399648332 2216 88-89 -0.7200901292437365 2216 90-91 -0.6308783475153312 2216 92-93 -0.6695467713302783 2216 94-95 -0.6959166247692892 2216 96-97 -0.6680929363099182 2216 98-99 -0.7667533620879752 2216 100 -0.760536597277504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 607.0 3 63.0 4 104.0 5 209.0 6 583.0 7 1523.0 8 3629.0 9 6407.0 10 8066.0 11 9778.0 12 13670.0 13 18197.0 14 20050.0 15 24845.0 16 33264.0 17 46314.0 18 46070.0 19 28284.0 20 23702.0 21 24498.0 22 26554.0 23 29540.0 24 35326.0 25 44686.0 26 58012.0 27 76448.0 28 100576.0 29 133323.0 30 174030.0 31 224901.0 32 291631.0 33 382549.0 34 530653.0 35 786873.0 36 1325301.0 37 2424238.0 38 2942946.0 39 930560.0 40 1917.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.71319588989417 19.501820365581384 13.955744885980625 23.829238858543814 2 16.04671942848738 20.446601348282403 38.03647060594222 25.470208617288005 3 18.286162039688726 25.08594933280714 29.998392417603554 26.629496209900584 4 13.407357224106867 17.1796725868974 36.741577297797114 32.67139289119862 5 13.79956528251373 36.746814278783226 34.471842632934845 14.981777805768198 6 31.399975235258122 36.84155172807021 17.505846999878948 14.252626036792723 7 27.808700834271548 31.888192782832235 21.790617794561314 18.5124885883349 8 26.25334501578451 34.79032483991801 19.87730471483674 19.07902542946074 9 25.473412701673798 16.423351699415885 20.65306626720568 37.450169331704636 10-11 24.65574329356052 26.27725006825992 27.991398279969935 21.075608358209617 12-13 25.29859376504293 23.6025001887401 28.293828741273224 22.805077304943744 14-15 22.537970250212236 25.409956633129347 25.76888798686159 26.283185129796827 16-17 22.250246931489013 28.654200531545598 26.246243395731106 22.849309141234286 18-19 22.24374591698465 27.373716807826465 28.56685064762683 21.815686627562055 20-21 22.977047394141696 26.5255622730536 28.545687066161584 21.951703266643122 22-23 22.76956714482009 26.327596668010784 28.566614530273384 22.33622165689575 24-25 22.573087546421153 26.476093135657568 28.466388911537045 22.484430406384238 26-27 22.484478077386413 26.532364099121835 28.468591817052836 22.51456600643892 28-29 22.514893586176843 26.4779815646727 28.579383390273616 22.42774145887684 30-31 22.611897568653973 26.67216501089989 28.38066683182629 22.33527058861985 32-33 22.44839033258176 26.57368724719062 28.537823928303833 22.440098491923788 34-35 22.574618700072374 26.376440614645013 28.538103584456625 22.510837100825988 36-37 22.77291855668437 26.494786436655843 28.299111028008046 22.433183978651744 38-39 22.630873275211293 26.639801878380652 28.282917495139735 22.44640735126832 40-41 22.631011440755923 26.436098688610194 28.388864278576325 22.54402559205756 42-43 22.600038144373638 26.552348351299248 28.233346243570985 22.61426726075613 44-45 22.612622295500724 26.69771861573072 28.0941329554172 22.595526133351356 46-47 22.79096431290581 26.66495751331946 27.86212663459719 22.68195153917754 48-49 22.808121699358104 26.719791806037748 27.72318047967382 22.74890601493033 50-51 22.780286904574208 26.797408011102586 27.587105430032526 22.835199654290676 52-53 22.801584649465468 26.814339888362802 27.523196652823206 22.860878809348524 54-55 22.80396459489939 26.987471777808647 27.533095039537965 22.675468587753997 56-57 22.663566978328344 26.823238354977413 27.58367431813351 22.929520348560736 58-59 22.662081715631775 26.541901544452344 27.735063305620915 23.060953434294962 60-61 22.763583536924394 26.777800446457572 27.623425305845966 22.835190710772068 62-63 22.62118632001384 26.82627364589164 27.693622855403753 22.85891717869076 64-65 22.659844573053277 26.620999009133133 27.73055568046681 22.98860073734678 66-67 22.55786184681173 26.658834623999855 27.72107272391417 23.062230805274247 68-69 22.727835564446192 26.742892252096794 27.58068147065819 22.948590712798822 70-71 22.733859702645784 26.557362608301894 27.6146057327151 23.094171956337224 72-73 22.552821178499613 26.758358280495763 27.60261901114591 23.08620152985871 74-75 22.581653571651664 26.7060558217403 27.51810972683965 23.194180879768385 76-77 22.650279875583358 26.728950397194286 27.39414573622853 23.226623990993826 78-79 22.62585643317513 26.769035439250956 27.50569724279303 23.099410884780884 80-81 22.50251781724474 26.683248664312607 27.543535592522016 23.270697925920633 82-83 22.489110761388194 26.67513154737647 27.571926593399066 23.26383109783627 84-85 22.509447316408114 26.661548071777908 27.405675730704044 23.423328881109935 86-87 22.530459594089997 26.759668357027284 27.390559545714392 23.31931250316833 88-89 22.396552293288686 26.646549350023076 27.49321721554146 23.463681141146782 90-91 22.338871160780286 26.578820075756788 27.504729201313584 23.577579562149346 92-93 22.390787036579624 26.7500671986727 27.375224922798857 23.483920841948812 94-95 22.323720698141994 26.50782001545 27.527296506833842 23.641162779574163 96-97 22.291661434179247 26.591851945968227 27.454833784189077 23.661652835663453 98-99 22.17339787651647 26.65862062215275 27.45420670759589 23.71377479373489 100 22.27472821936802 26.457269955310654 27.427299708196053 23.84070211712528 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1954.0 1 1494.5 2 5211.5 3 7812.5 4 3932.5 5 1641.0 6 2748.0 7 6441.5 8 11317.0 9 13124.5 10 9559.0 11 5582.0 12 4829.0 13 5247.0 14 5907.5 15 6601.5 16 7294.5 17 8158.0 18 9169.5 19 10267.5 20 11770.0 21 14010.5 22 17428.0 23 22836.5 24 30322.0 25 40018.0 26 53354.0 27 70556.5 28 91009.5 29 113865.0 30 135250.5 31 155780.0 32 176643.0 33 194737.0 34 212128.0 35 231540.5 36 253598.0 37 277413.5 38 300510.5 39 317921.5 40 333970.0 41 351207.0 42 361361.0 43 366885.0 44 371622.0 45 375427.5 46 378353.5 47 374238.5 48 368417.5 49 367481.5 50 367318.5 51 363410.0 52 352860.0 53 345318.5 54 338908.5 55 324044.0 56 305670.5 57 281706.5 58 256004.0 59 237130.5 60 215533.5 61 186550.0 62 158414.0 63 132614.5 64 107800.0 65 87551.5 66 69099.5 67 52834.5 68 40265.5 69 30129.5 70 22363.0 71 16404.5 72 12256.0 73 9018.0 74 6771.5 75 4845.5 76 3084.5 77 2028.5 78 1362.5 79 808.5 80 412.0 81 180.5 82 88.0 83 52.5 84 31.0 85 18.0 86 14.5 87 10.5 88 8.5 89 6.5 90 3.5 91 2.5 92 1.5 93 1.0 94 1.0 95 0.0 96 0.0 97 1.5 98 3.0 99 3.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08839394762310032 2 0.0 3 0.0 4 0.0 5 0.007617779879772043 6 1.2927141614158617E-4 7 0.0 8 7.386938065233496E-5 9 0.0 10-11 0.0 12-13 0.014261407302191418 14-15 9.23367258154187E-6 16-17 0.0 18-19 1.846734516308374E-5 20-21 1.846734516308374E-5 22-23 0.0 24-25 3.693469032616748E-5 26-27 2.3084181453854676E-5 28-29 1.2465457985081523E-4 30-31 0.0 32-33 3.231785403539654E-5 34-35 9.23367258154187E-6 36-37 1.846734516308374E-5 38-39 1.2465457985081523E-4 40-41 4.1551526616938414E-5 42-43 1.8929028792160832E-4 44-45 1.0157039839696057E-4 46-47 3.231785403539654E-5 48-49 2.4007548712008864E-4 50-51 1.8005661534006647E-4 52-53 2.216081419570049E-4 54-55 1.708229427585246E-4 56-57 1.9390712421237929E-4 58-59 3.693469032616748E-5 60-61 6.001887178002216E-5 62-63 3.0471119519088173E-4 64-65 1.4773876130466993E-4 66-67 5.309361734386575E-4 68-69 5.078519919848029E-4 70-71 1.3850508872312805E-4 72-73 3.5087955809859106E-4 74-75 3.924310847155295E-4 76-77 2.3545865082931768E-4 78-79 0.0011726764178558175 80-81 0.0010157039839696058 82-83 7.710116605587462E-4 84-85 0.001218844780763527 86-87 8.125631871756845E-4 88-89 0.0011034238734942536 90-91 0.001278863652543549 92-93 9.233672581541871E-4 94-95 0.0014127519049759061 96-97 0.0013942845598128224 98-99 0.0016158927017698272 100 4.893846468217192E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0829927E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.40924101539698 #Duplication Level Percentage of deduplicated Percentage of total 1 71.18469461386256 35.171817327837665 2 15.57767852417895 15.393625453230628 3 5.260965912164414 7.798209981837581 4 2.383154750783575 4.70999469833816 5 1.4115554694002732 3.4871942197099957 6 0.8758263053230861 2.5964347804399814 7 0.609199382979415 2.1070055398042733 8 0.4372273593810455 1.7282457582546935 9 0.33635842000657457 1.495729281749667 >10 1.7480078121173865 15.546044936625677 >50 0.12146425123147878 4.127032950809611 >100 0.05208273590466611 4.360411353198777 >500 0.001409576513788083 0.4521548717982393 >1k 2.624205378384578E-4 0.2306093204204968 >5k 3.7488538274882536E-5 0.10992356983029157 >10k+ 7.497707654976507E-5 0.6855659561140368 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25817 0.23838572503766647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22981 0.2121990295964137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13504 0.12469151454114141 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11722 0.10823711000083379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.23367258154187E-6 0.0 2 0.0 0.0 0.0 9.23367258154187E-6 0.0 3 0.0 0.0 0.0 9.23367258154187E-6 0.0 4 0.0 0.0 0.0 9.23367258154187E-6 0.0 5 0.0 0.0 0.0 1.846734516308374E-5 0.0 6 0.0 0.0 0.0 1.846734516308374E-5 0.0 7 0.0 0.0 0.0 4.616836290770935E-5 0.0 8 0.0 0.0 0.0 4.616836290770935E-5 0.0 9 0.0 0.0 0.0 4.616836290770935E-5 0.0 10-11 0.0 9.23367258154187E-6 0.0 5.0785199198480284E-5 0.0 12-13 0.0 3.231785403539654E-5 0.0 6.0018871780022154E-5 0.0 14-15 0.0 6.001887178002216E-5 0.0 8.310305323387683E-5 0.0 16-17 0.0 7.84862169431059E-5 0.0 8.771988952464777E-5 0.0 18-19 0.0 8.310305323387683E-5 0.0 1.2465457985081523E-4 0.0 20-21 0.0 8.310305323387683E-5 0.0 2.0775763308469207E-4 0.0 22-23 0.0 8.310305323387683E-5 0.0 3.5087955809859106E-4 4.616836290770935E-6 24-25 0.0 8.771988952464777E-5 0.0 7.294601339418076E-4 9.23367258154187E-6 26-27 0.0 9.23367258154187E-5 0.0 0.001278863652543549 9.23367258154187E-6 28-29 0.0 9.695356210618963E-5 0.0 0.002576194650250182 9.23367258154187E-6 30-31 0.0 1.1080407097850245E-4 0.0 0.006491271824823935 9.23367258154187E-6 32-33 0.0 1.2003774356004431E-4 0.0 0.014543034315928446 9.23367258154187E-6 34-35 9.23367258154187E-6 1.338882524323571E-4 0.0 0.02828273911726275 9.23367258154187E-6 36-37 9.23367258154187E-6 1.7543977904929553E-4 0.0 0.05087291908800493 1.3850508872312806E-5 38-39 9.23367258154187E-6 2.12374469375463E-4 0.0 0.08515754538326989 1.846734516308374E-5 40-41 9.23367258154187E-6 2.216081419570049E-4 0.0 0.12383278299105802 1.846734516308374E-5 42-43 9.23367258154187E-6 2.3084181453854674E-4 0.0 0.16448864336758687 1.846734516308374E-5 44-45 1.846734516308374E-5 2.3545865082931768E-4 0.0 0.21310392950940482 1.846734516308374E-5 46-47 2.770101774462561E-5 2.4007548712008862E-4 0.0 0.27388457927740417 1.846734516308374E-5 48-49 2.770101774462561E-5 2.4930915970163047E-4 0.0 0.33946212195151454 1.846734516308374E-5 50-51 2.770101774462561E-5 2.5854283228317235E-4 0.0 0.40965188408010506 1.846734516308374E-5 52-53 2.770101774462561E-5 2.631596685739433E-4 0.0 0.4829118423420582 1.846734516308374E-5 54-55 2.770101774462561E-5 2.86243850027798E-4 0.0 0.5629308489337002 1.846734516308374E-5 56-57 3.231785403539654E-5 3.0471119519088173E-4 0.0 0.658143863758269 1.846734516308374E-5 58-59 3.693469032616748E-5 3.0932803148165267E-4 0.0 0.7641972101935682 1.846734516308374E-5 60-61 3.693469032616748E-5 3.139448677724236E-4 0.0 0.8775220737868317 1.846734516308374E-5 62-63 3.693469032616748E-5 3.139448677724236E-4 0.0 0.9936955253715006 1.846734516308374E-5 64-65 3.693469032616748E-5 3.139448677724236E-4 0.0 1.1173528685835095 1.846734516308374E-5 66-67 3.693469032616748E-5 3.139448677724236E-4 0.0 1.2593944539053679 1.846734516308374E-5 68-69 3.693469032616748E-5 3.139448677724236E-4 0.0 1.4156420444939286 1.846734516308374E-5 70-71 4.155152661693842E-5 3.2317854035396543E-4 0.0 1.5850060669845698 1.846734516308374E-5 72-73 4.616836290770935E-5 3.2779537664473637E-4 0.0 1.7631420784276755 1.846734516308374E-5 74-75 4.616836290770935E-5 3.324122129355073E-4 0.0 1.9468829291277772 1.846734516308374E-5 76-77 4.616836290770935E-5 3.324122129355073E-4 0.0 2.14428499841227 1.846734516308374E-5 78-79 4.616836290770935E-5 3.324122129355073E-4 0.0 2.363954069127151 1.846734516308374E-5 80-81 4.616836290770935E-5 3.324122129355073E-4 0.0 2.600299152524297 1.846734516308374E-5 82-83 4.616836290770935E-5 3.3702904922627825E-4 0.0 2.8476969420015483 1.846734516308374E-5 84-85 4.616836290770935E-5 3.5087955809859106E-4 0.0 3.0971907751548096 1.846734516308374E-5 86-87 4.616836290770935E-5 3.6011323068013294E-4 0.0 3.3579727730390054 2.3084181453854676E-5 88 4.616836290770935E-5 3.6011323068013294E-4 0.0 3.5742807869342057 2.770101774462561E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15410 0.0 49.665516 1 GTATCAA 26650 0.0 37.614586 1 TATAACG 1180 0.0 34.254795 2 ATCAACG 31475 0.0 31.015282 3 TCAACGC 31635 0.0 30.947557 4 CAACGCA 33225 0.0 29.551422 5 TAACGCA 1505 0.0 29.35596 4 TATCAAC 33740 0.0 29.072495 2 AACGCAG 34705 0.0 28.393208 6 ATAACGC 1650 0.0 26.776194 3 TACCTGG 8865 0.0 25.501825 2 GTACCTG 9435 0.0 24.729185 1 ACGCAGA 40125 0.0 24.5578 7 CGCAGAG 41575 0.0 23.735228 8 GTACATG 33115 0.0 21.5918 1 GTGGTAT 7745 0.0 20.893301 1 GCAGAGT 47560 0.0 20.689053 9 TATACCG 875 0.0 20.41176 5 TACATGG 33950 0.0 20.240095 2 GTATAAC 3315 0.0 19.866188 1 >>END_MODULE