FastQCFastQC Report
Wed 25 May 2016
SRR2049431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.037.725691
TATAACG5850.036.1531752
GTATCAA84400.031.9842341
TCAACGC88850.029.3581734
TACCTGG50550.029.1013362
ATCAACG90000.028.9830443
CAACGCA97100.026.9128865
GTACCTG57100.026.6855031
AACGCAG104500.025.3671256
TATCAAC105450.025.0483722
ATAACGC9400.023.4997653
TAACGCA9500.023.2523984
GTACATG184850.022.8721351
ACGCAGA121250.021.8631787
TACATGG182600.021.6206262
TATCACG8500.021.5643022
CGCAGAG124650.021.1141838
ACATGGG187900.020.3606243
ACCTGGG71900.020.067953
GTATAAC17250.019.629531
Produced by FastQC (version 0.11.5)