##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049431_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5810060
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957361541877365	31.0	31.0	34.0	28.0	34.0
2	31.068107902500145	31.0	31.0	34.0	28.0	34.0
3	31.149635804105294	33.0	31.0	34.0	28.0	34.0
4	34.73653215285212	37.0	35.0	37.0	32.0	37.0
5	34.74482053541616	37.0	35.0	37.0	32.0	37.0
6	34.78474008874263	37.0	35.0	37.0	32.0	37.0
7	34.654533516004996	37.0	35.0	37.0	32.0	37.0
8	34.6536063310878	37.0	35.0	37.0	32.0	37.0
9	36.23498535299119	39.0	37.0	39.0	32.0	39.0
10-11	36.17399217908249	39.0	37.0	39.0	32.0	39.0
12-13	36.12387798060605	39.0	37.0	39.0	32.0	39.0
14-15	37.371328006939684	40.0	38.0	41.0	32.0	41.0
16-17	37.35503635074336	40.0	38.0	41.0	32.0	41.0
18-19	37.35381579536184	40.0	38.0	41.0	32.0	41.0
20-21	37.37987189461039	40.0	38.0	41.0	32.0	41.0
22-23	37.32879376460828	40.0	38.0	41.0	32.0	41.0
24-25	37.315189171884626	40.0	38.0	41.0	31.5	41.0
26-27	37.29637611315546	40.0	38.0	41.0	31.5	41.0
28-29	37.25981685214955	40.0	38.0	41.0	31.0	41.0
30-31	37.201900410667015	40.0	38.0	41.0	31.0	41.0
32-33	37.16012373366196	40.0	37.5	41.0	31.0	41.0
34-35	37.09048503802026	40.0	37.0	41.0	31.0	41.0
36-37	36.99034648523423	40.0	37.0	41.0	30.5	41.0
38-39	36.88602492917457	40.0	37.0	41.0	30.0	41.0
40-41	36.762419751259024	40.0	37.0	41.0	30.0	41.0
42-43	36.66481275580631	40.0	37.0	41.0	30.0	41.0
44-45	36.510300409978555	40.0	36.0	41.0	30.0	41.0
46-47	36.37654568111173	40.0	36.0	41.0	30.0	41.0
48-49	36.25321571550036	40.0	36.0	41.0	30.0	41.0
50-51	36.11058121602875	39.5	35.0	41.0	29.0	41.0
52-53	35.96809094914683	39.0	35.0	41.0	29.0	41.0
54-55	35.791888207694925	39.0	35.0	41.0	28.5	41.0
56-57	35.59272666719448	39.0	35.0	41.0	28.0	41.0
58-59	35.36750722023525	38.5	35.0	40.5	28.0	41.0
60-61	35.130981435647826	38.0	35.0	40.0	28.0	41.0
62-63	34.833772284623564	37.5	34.0	40.0	27.5	41.0
64-65	34.56165418257299	37.0	34.0	40.0	27.0	41.0
66-67	34.2999606716626	37.0	34.0	40.0	26.5	41.0
68-69	33.98378063221378	36.0	34.0	39.0	26.0	41.0
70-71	33.65200961780085	36.0	34.0	39.0	26.0	41.0
72-73	33.306862493674764	35.0	33.0	38.5	26.0	40.0
74-75	33.00706868775882	35.0	33.0	37.5	26.0	40.0
76-77	32.694135688788066	35.0	33.0	37.0	25.5	39.0
78-79	32.37116527195933	35.0	33.0	36.5	25.0	39.0
80-81	32.06373273253632	35.0	33.0	36.0	24.5	39.0
82-83	31.793886121657952	35.0	33.0	36.0	24.0	37.0
84-85	31.537851588451755	35.0	33.0	35.0	23.5	37.0
86-87	31.29346039455703	35.0	32.0	35.0	23.0	36.5
88-89	31.08469860896445	35.0	32.0	35.0	20.5	36.0
90-91	30.891297594172865	35.0	32.0	35.0	20.0	36.0
92-93	30.68660719510642	35.0	32.0	35.0	18.5	36.0
94-95	30.480204421296854	35.0	32.0	35.0	14.0	35.5
96-97	30.279879811912444	35.0	32.0	35.0	4.5	35.0
98-99	30.0879132057156	35.0	32.0	35.0	2.0	35.0
100	29.94682808783386	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2651759296823677
1101	2	0.2914912370472038
1101	3	0.3192852642233568
1101	4	0.31844036738971937
1101	5	0.31925339108501305
1101	6	0.3442782157188162
1101	7	0.34479544390597994
1101	8	0.31562166754552123
1101	9	0.3418573663866056
1101	10-11	0.44966571699742275
1101	12-13	0.41680867481699835
1101	14-15	0.5597106967617265
1101	16-17	0.49304651184938564
1101	18-19	0.5279777037330788
1101	20-21	0.5400794954302768
1101	22-23	0.5282581439474967
1101	24-25	0.49484763901521944
1101	26-27	0.5447249800560385
1101	28-29	0.5430860267755122
1101	30-31	0.530837318455923
1101	32-33	0.5357391593749554
1101	34-35	0.5144351625015702
1101	36-37	0.5341146113963404
1101	38-39	0.5134255579601827
1101	40-41	0.5506330035254905
1101	42-43	0.4662994456023739
1101	44-45	0.5045133711815808
1101	46-47	0.4519136165145383
1101	48-49	0.43678303717405953
1101	50-51	0.44598692932562045
1101	52-53	0.37835604154741986
1101	54-55	0.3493923148495952
1101	56-57	0.37607688856200383
1101	58-59	0.33077747240660216
1101	60-61	0.318735147769317
1101	62-63	0.34098166870133184
1101	64-65	0.3127026706427216
1101	66-67	0.2923138442960962
1101	68-69	0.2547382419092159
1101	70-71	0.26780215721577605
1101	72-73	0.28637018267593106
1101	74-75	0.2492545490708693
1101	76-77	0.23650003562241295
1101	78-79	0.20949366895005284
1101	80-81	0.28908799203085067
1101	82-83	0.2541747896221267
1101	84-85	0.2580956172007127
1101	86-87	0.2476744709499492
1101	88-89	0.2094617913091419
1101	90-91	0.23487333617879003
1101	92-93	0.22099343789096082
1101	94-95	0.23574753693635842
1101	96-97	0.29270608648036855
1101	98-99	0.27102760087959865
1101	100	0.20616092536915076
1102	1	0.20296359812711273
1102	2	0.23938281406114115
1102	3	0.22110537438479483
1102	4	0.23074430785059974
1102	5	0.2545161640322746
1102	6	0.26497277141911013
1102	7	0.22265180144320595
1102	8	0.2957543041201234
1102	9	0.34145060384069836
1102	10-11	0.305788761641999
1102	12-13	0.31419828771618796
1102	14-15	0.4265804995285194
1102	16-17	0.36736341509421067
1102	18-19	0.35160913981814446
1102	20-21	0.3732140224475913
1102	22-23	0.3640127992786546
1102	24-25	0.3503253102688788
1102	26-27	0.29788427625876324
1102	28-29	0.33550401394220586
1102	30-31	0.3293642752639485
1102	32-33	0.29306051263471744
1102	34-35	0.31251625730686783
1102	36-37	0.35970093218856647
1102	38-39	0.4003700153710099
1102	40-41	0.32995848950169204
1102	42-43	0.30307416409870314
1102	44-45	0.2904979273127708
1102	46-47	0.22415834309146732
1102	48-49	0.13416431419724972
1102	50-51	0.13057528872186452
1102	52-53	0.1162303345182707
1102	54-55	0.14444012507249226
1102	56-57	0.08598692151306864
1102	58-59	0.10309180883618296
1102	60-61	0.0921431953573304
1102	62-63	0.05149382527530122
1102	64-65	0.04158783824615142
1102	66-67	0.09713718373615876
1102	68-69	0.06722071625872417
1102	70-71	0.0034399692684914385
1102	72-73	0.03193206983301167
1102	74-75	0.10576315602308739
1102	76-77	0.03387570571939591
1102	78-79	0.054652384621988404
1102	80-81	0.057021522761957044
1102	82-83	0.06754952479341014
1102	84-85	0.046205284895549426
1102	86-87	0.02013570275686405
1102	88-89	0.0388948496659296
1102	90-91	0.03356411009629312
1102	92-93	-0.010254001915864563
1102	94-95	-7.487806078927406E-4
1102	96-97	0.02513997046064631
1102	98-99	0.0758420307713017
1102	100	0.013589851009498943
1103	1	0.3152129745769656
1103	2	0.33287031395941113
1103	3	0.3265655592317316
1103	4	0.35089108532899616
1103	5	0.30423885063166267
1103	6	0.2925551473728234
1103	7	0.3201855018661135
1103	8	0.30596791966374326
1103	9	0.3537907315404567
1103	10-11	0.37095622073137946
1103	12-13	0.41006515947147193
1103	14-15	0.4877364923280041
1103	16-17	0.420788760367401
1103	18-19	0.4036096674345444
1103	20-21	0.4723076078697801
1103	22-23	0.473061290082299
1103	24-25	0.5037166276622429
1103	26-27	0.49943265995749897
1103	28-29	0.5410914808721259
1103	30-31	0.5244707636404939
1103	32-33	0.5194984477627784
1103	34-35	0.46281725663015294
1103	36-37	0.5033293268573118
1103	38-39	0.49812739228828207
1103	40-41	0.4733304159173315
1103	42-43	0.47984916103911246
1103	44-45	0.41877136405338433
1103	46-47	0.44154364909747557
1103	48-49	0.42409467221017394
1103	50-51	0.3891727949512216
1103	52-53	0.41683263259789527
1103	54-55	0.3783820906462907
1103	56-57	0.35490941702810375
1103	58-59	0.39721996495658374
1103	60-61	0.38229766749753935
1103	62-63	0.34205128824503106
1103	64-65	0.44620103446064263
1103	66-67	0.38671596127228725
1103	68-69	0.38196689606873946
1103	70-71	0.32387963834429456
1103	72-73	0.31575453858332736
1103	74-75	0.3782010263507658
1103	76-77	0.26518069487300977
1103	78-79	0.23576050037881657
1103	80-81	0.2938245558499233
1103	82-83	0.3125796500623821
1103	84-85	0.2752177377789913
1103	86-87	0.27123358558087673
1103	88-89	0.2528549332430714
1103	90-91	0.2872775099713252
1103	92-93	0.2481115358267516
1103	94-95	0.31013921192685245
1103	96-97	0.34532912923341286
1103	98-99	0.38851122772408786
1103	100	0.3479476446454548
1104	1	0.1401708605000067
1104	2	0.16969105243993354
1104	3	0.12898426418806608
1104	4	0.19617490965071482
1104	5	0.17348191603493746
1104	6	0.212692182639735
1104	7	0.21027898599155748
1104	8	0.2308752820486788
1104	9	0.29530134526715557
1104	10-11	0.32322089937719767
1104	12-13	0.34440971041514956
1104	14-15	0.42100507738553006
1104	16-17	0.41326442560510657
1104	18-19	0.37427046337985104
1104	20-21	0.3929753925335646
1104	22-23	0.376636820842819
1104	24-25	0.32545131216760126
1104	26-27	0.32774157704296414
1104	28-29	0.32356200917918443
1104	30-31	0.34758123153891063
1104	32-33	0.35520047937285426
1104	34-35	0.3995581145035061
1104	36-37	0.4098105100666416
1104	38-39	0.41779586439862015
1104	40-41	0.4508176169919551
1104	42-43	0.3839028922654606
1104	44-45	0.32468347861851754
1104	46-47	0.32897540337116027
1104	48-49	0.29062958694261454
1104	50-51	0.32872184158467377
1104	52-53	0.2942957589469515
1104	54-55	0.2622378838562014
1104	56-57	0.2410438811588378
1104	58-59	0.3274756481869048
1104	60-61	0.33637273583044447
1104	62-63	0.3295580117334822
1104	64-65	0.34638267437011905
1104	66-67	0.3057765706737001
1104	68-69	0.24292979161203476
1104	70-71	0.2722371633764453
1104	72-73	0.19762361421624774
1104	74-75	0.1879109158919121
1104	76-77	0.18449135498343594
1104	78-79	0.19894800443145755
1104	80-81	0.251499340954247
1104	82-83	0.20104347187053406
1104	84-85	0.17687732843001314
1104	86-87	0.17642909683531016
1104	88-89	0.22863490612802906
1104	90-91	0.2299695806544264
1104	92-93	0.24122055553211652
1104	94-95	0.22382965888285256
1104	96-97	0.2808111794296231
1104	98-99	0.35691069100237627
1104	100	0.29335604918037816
1105	1	0.2225246795838558
1105	2	0.19941736834925194
1105	3	0.31783096016508594
1105	4	0.27656323854301945
1105	5	0.2648674994338478
1105	6	0.30746543235948565
1105	7	0.28970284796854173
1105	8	0.2633813045402249
1105	9	0.3494362844123202
1105	10-11	0.3899253112411927
1105	12-13	0.33722271979903695
1105	14-15	0.4068135401385078
1105	16-17	0.305236167178748
1105	18-19	0.32143697668373505
1105	20-21	0.2980431377379418
1105	22-23	0.3335413923674153
1105	24-25	0.3077110550451323
1105	26-27	0.3466219383664324
1105	28-29	0.3701493248234655
1105	30-31	0.368719562691723
1105	32-33	0.37292267394420975
1105	34-35	0.4013462926881459
1105	36-37	0.36528686065275195
1105	38-39	0.4154118017184487
1105	40-41	0.4309648679687115
1105	42-43	0.42741923640166135
1105	44-45	0.4315647686431845
1105	46-47	0.3737188653350145
1105	48-49	0.33532381354032736
1105	50-51	0.3847041276055805
1105	52-53	0.35678499814439135
1105	54-55	0.2691475032636532
1105	56-57	0.25714731033092875
1105	58-59	0.2700051675202104
1105	60-61	0.24366426642600914
1105	62-63	0.2674295851119268
1105	64-65	0.2138413706058344
1105	66-67	0.21981369862485423
1105	68-69	0.18192893393914034
1105	70-71	0.24650627227252642
1105	72-73	0.29445109278891124
1105	74-75	0.2767897219528237
1105	76-77	0.19434539303888698
1105	78-79	0.1998493925769651
1105	80-81	0.2729338965077446
1105	82-83	0.18376114677903388
1105	84-85	0.12899630861766198
1105	86-87	0.14279661948001987
1105	88-89	0.14318476471327912
1105	90-91	0.20632097896829293
1105	92-93	0.15457170186153846
1105	94-95	0.2226912365907019
1105	96-97	0.21109779668065798
1105	98-99	0.20819947235260017
1105	100	0.15015663273662483
1106	1	0.1540431607981283
1106	2	0.19226976514074678
1106	3	0.16087636854725318
1106	4	0.19448029794460098
1106	5	0.17961622390828325
1106	6	0.19128746557841936
1106	7	0.1934814091079673
1106	8	0.16015049303559437
1106	9	0.23096088210860444
1106	10-11	0.2688348889685912
1106	12-13	0.24382990929304782
1106	14-15	0.2702803097836437
1106	16-17	0.2443012766130721
1106	18-19	0.26457166172790636
1106	20-21	0.2725214861494578
1106	22-23	0.2565273811730009
1106	24-25	0.3362927871319741
1106	26-27	0.28541952232986034
1106	28-29	0.24386116050413875
1106	30-31	0.2834422449246574
1106	32-33	0.2403391353249802
1106	34-35	0.2625587480777227
1106	36-37	0.2769235372670025
1106	38-39	0.30387451414770084
1106	40-41	0.3882378514800564
1106	42-43	0.3421767449654993
1106	44-45	0.28881257523195103
1106	46-47	0.27039002339270723
1106	48-49	0.2583584903143503
1106	50-51	0.26847804468305725
1106	52-53	0.3011785430967038
1106	54-55	0.2578883330532449
1106	56-57	0.17762040884702657
1106	58-59	0.23129304482372248
1106	60-61	0.19635960259676466
1106	62-63	0.2800436683868597
1106	64-65	0.22809353154005407
1106	66-67	0.2713575295499169
1106	68-69	0.2438179970768104
1106	70-71	0.22083853611025006
1106	72-73	0.2125664039210804
1106	74-75	0.16154902784469272
1106	76-77	0.15553776834456556
1106	78-79	0.1367149453577099
1106	80-81	0.1660805265356018
1106	82-83	0.1472821049549431
1106	84-85	0.17276923112953568
1106	86-87	0.1551372041032657
1106	88-89	0.10512112138825813
1106	90-91	0.09597495170947923
1106	92-93	0.00553665922846136
1106	94-95	0.005605825837449174
1106	96-97	0.10498542493215268
1106	98-99	0.0356490021440834
1106	100	0.04980539041560661
1107	1	0.30120479848023507
1107	2	0.23318781284733348
1107	3	0.3001647218893737
1107	4	0.25931947851908177
1107	5	0.21199650169804585
1107	6	0.2730724199550494
1107	7	0.2441806149259378
1107	8	0.21530105642194997
1107	9	0.27445789922936825
1107	10-11	0.2755463672437415
1107	12-13	0.28602839635332344
1107	14-15	0.3385551591089282
1107	16-17	0.3267534997522006
1107	18-19	0.3728316697156444
1107	20-21	0.40501451774836283
1107	22-23	0.43217509805622
1107	24-25	0.4172934049991994
1107	26-27	0.49808099928299754
1107	28-29	0.40653088264480175
1107	30-31	0.4539749884081701
1107	32-33	0.4318637087669899
1107	34-35	0.4271013802198951
1107	36-37	0.42619274229176796
1107	38-39	0.3797523309055393
1107	40-41	0.4907486676353372
1107	42-43	0.49872450234407495
1107	44-45	0.45330771926876423
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2216	90-91	-0.9384727594753919
2216	92-93	-0.9031514110910308
2216	94-95	-0.841517431406654
2216	96-97	-0.9312517925739492
2216	98-99	-0.9410176112294764
2216	100	-0.986800977006471
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104784.0
3	17622.0
4	11294.0
5	9434.0
6	8099.0
7	8269.0
8	8923.0
9	10006.0
10	11286.0
11	12365.0
12	13280.0
13	14248.0
14	15273.0
15	15821.0
16	16835.0
17	17759.0
18	17253.0
19	16487.0
20	16634.0
21	17830.0
22	20533.0
23	24314.0
24	29581.0
25	35591.0
26	43509.0
27	50854.0
28	62385.0
29	78533.0
30	95929.0
31	115618.0
32	149031.0
33	197919.0
34	276658.0
35	415621.0
36	719561.0
37	1271990.0
38	1428669.0
39	429456.0
40	806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47082897689985	18.767394872872526	13.133118253269693	23.628657896957932
2	16.771397385212065	19.786833037746266	38.27630766508364	25.165461911958033
3	18.647758948978048	24.989779014987967	29.433028405128685	26.929433630905297
4	13.386976808715842	16.898107864284277	37.17143070095759	32.543484626042286
5	14.091211883880852	36.46315119071185	33.88360544276235	15.562031482644956
6	31.412750039761256	36.397313722830674	17.98520261715111	14.204733620256965
7	28.245016889536473	31.423489590667597	21.758994181708758	18.57249933808717
8	26.36547799927671	34.27392681613183	20.183425282366528	19.177169902224936
9	25.585139380703104	17.09719296965207	20.687725533586388	36.62994211605844
10-11	24.704084947153323	26.183724762464944	27.910268148621714	21.201922141760022
12-13	25.23325971434916	23.7739168455499	27.779019105933873	23.213804334167072
14-15	22.805906840716023	25.751245610316136	25.03102030354928	26.41182724541856
16-17	22.775134005829045	28.757837250621005	25.36803781417749	23.098990929372455
18-19	22.74234063873345	27.945920261553	27.029446830865396	22.282292268848156
20-21	23.761276691555373	27.270236764045684	26.545359463263217	22.423127081135718
22-23	23.46510872903191	27.22598094367243	26.49086706428171	22.81804326301395
24-25	23.179782371589184	27.29657672148576	26.504561410515244	23.019079496409812
26-27	23.037633746942	27.23695595634862	26.571291511403484	23.154118785305897
28-29	23.08004600832033	27.23625258719341	26.643022737006195	23.040678667480062
30-31	23.250601344491642	27.651071128200417	26.29566629176233	22.802661235545614
32-33	22.88386101663493	27.393815320641828	26.610339862065974	23.111983800657264
34-35	23.12944701476551	26.9485749026329	26.771281703316014	23.15069637928558
36-37	23.44432180989319	27.1886939512095	26.46603179761673	22.90095244128058
38-39	23.179108862221216	27.39453221597319	26.46567875531536	22.96068016649023
40-41	23.100325010599292	27.14100151325045	26.695030716866896	23.063642759283358
42-43	22.96432538984464	27.227210307314316	26.673439339082645	23.1350249637584
44-45	23.069876864895683	27.375103806156893	26.50428548999949	23.05073383894793
46-47	23.183289711697828	27.370510534952054	26.39856919658381	23.04763055676631
48-49	23.08163587111452	27.186367717759698	26.60469913913842	23.127297271987363
50-51	23.046099531887023	27.166268324527348	26.54235006657205	23.24528207701358
52-53	22.975376780969576	27.177399476303886	26.55678412304311	23.29043961968343
54-55	23.072227120877436	27.489632136096258	26.502992769929172	22.93514797309713
56-57	22.841505069492758	27.268020557024897	26.524766374165086	23.365707999317255
58-59	22.873733398486078	26.901883789789803	26.707776693619913	23.516606118104207
60-61	23.113169062158867	27.233580886461212	26.472865374956413	23.180384676423515
62-63	22.880497637243884	27.340070162706247	26.541639945455593	23.23779225459428
64-65	22.97653043262318	27.030876569899338	26.572813072821468	23.419779924656023
66-67	22.813515847358655	27.08385207381867	26.545090680211974	23.557541398610702
68-69	23.103000803630685	27.224308498474116	26.46154359922403	23.211147098671166
70-71	23.096310023005437	26.913675124947773	26.574946196407517	23.415068655639278
72-73	22.805819443955492	27.086625871259223	26.77637809738857	23.331176587396712
74-75	22.809492716112143	26.987999656390336	26.74208462610156	23.46042300139596
76-77	22.952661894341848	27.05879770980048	26.49651542829356	23.492024967564113
78-79	22.980828782405457	27.15702448326327	26.64719031341758	23.214956420913694
80-81	22.835278821784133	26.981542713595953	26.7632503450493	23.41992811957061
82-83	22.777105970952125	26.99762022592792	26.825742872512105	23.399530930607852
84-85	22.840478355876094	26.952638554435666	26.62864866246586	23.57823442722238
86-87	23.0240138402348	27.04141741907164	26.60547584414243	23.32909289655113
88-89	22.805160998684077	26.95796142841441	26.726975347570058	23.50990222533145
90-91	22.69464612920615	26.846464554134446	26.78062920825702	23.678260108402384
92-93	22.90977867070227	27.006808013737206	26.639718852717493	23.443694462843034
94-95	22.934798456862325	26.703258605354296	26.7895785359885	23.572364401794875
96-97	22.871637590884415	26.80447668239661	26.73017343563822	23.593712291080752
98-99	22.829243984317074	26.80541061820698	26.752075586506763	23.613269810969186
100	22.928759267718956	26.71619548195581	26.68290622447149	23.67213902585374
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2898.0
1	2146.0
2	2062.0
3	2709.0
4	2403.5
5	1962.5
6	1726.5
7	1653.0
8	1743.0
9	1745.5
10	1483.0
11	1313.5
12	1301.5
13	1305.5
14	1370.5
15	1503.5
16	1665.5
17	1853.5
18	2287.5
19	2948.5
20	3751.5
21	5023.5
22	7230.0
23	10701.5
24	15157.5
25	20576.5
26	28269.0
27	38023.0
28	49538.0
29	61272.0
30	71257.0
31	81168.0
32	91373.0
33	100764.5
34	109995.0
35	120681.0
36	131605.5
37	142709.0
38	154340.0
39	162953.0
40	171215.5
41	179661.5
42	184795.0
43	187733.0
44	189851.5
45	192647.0
46	196318.5
47	197006.5
48	196915.0
49	199496.5
50	201822.0
51	201197.0
52	196054.5
53	192247.5
54	190331.0
55	184179.5
56	173604.5
57	159641.0
58	145789.0
59	134205.5
60	122419.5
61	108592.0
62	93011.5
63	78013.0
64	64350.5
65	53051.0
66	42461.5
67	32383.5
68	24689.0
69	18544.5
70	13853.5
71	10295.0
72	7687.5
73	5809.0
74	4621.0
75	3470.5
76	2369.0
77	1729.5
78	1290.0
79	898.0
80	599.5
81	431.0
82	329.5
83	265.5
84	209.5
85	169.5
86	154.0
87	118.0
88	89.5
89	75.5
90	63.5
91	56.0
92	42.5
93	26.5
94	18.0
95	18.5
96	21.5
97	19.0
98	15.0
99	16.0
100	29.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07693552218049383
2	0.01234066429606579
3	0.016058353958478915
4	0.010206435045421217
5	0.008777878369586545
6	0.006609226066512222
7	0.019586716832528408
8	0.008622974633652664
9	0.008674609212297292
10-11	0.012168549033917033
12-13	0.014974027806941752
14-15	0.00961263739100801
16-17	0.012125520218379844
18-19	0.007805427138446074
20-21	0.012598837189288923
22-23	0.013011913818445936
24-25	0.015352681383669015
26-27	0.005180669390677549
28-29	0.01163499172125589
30-31	0.009948262152198083
32-33	0.011850135798941836
34-35	0.008166869188958462
36-37	0.009776146890049327
38-39	0.018424938813024306
40-41	0.012237395138776537
42-43	0.007538648482115504
44-45	0.019896524304396167
46-47	0.017486910634313586
48-49	0.017142680110016077
50-51	0.009724512311404702
52-53	0.010051531309487338
54-55	0.011058405593057558
56-57	0.019715803279139972
58-59	0.002117017724429696
60-61	0.00685018743352048
62-63	0.01460397999332193
64-65	0.023536762098842354
66-67	0.012332058532958353
68-69	0.013760615208793025
70-71	0.024664117065916705
72-73	0.019870707015073857
74-75	0.019672774463602785
76-77	0.00899302244727249
78-79	0.010705569305652609
80-81	0.0190617652829747
82-83	0.01178989545718977
84-85	0.01066254049011542
86-87	0.015662488855536776
88-89	0.014380230152528546
90-91	0.01498263357004919
92-93	0.007504225429685751
94-95	0.014750277966148372
96-97	0.013046336870875688
98-99	0.009595425864793134
100	0.006505956909222968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5810060.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.002123946215114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.61881128515384	47.172956592358965
2	12.561241714214772	15.074023645093645
3	3.7169722989132987	6.6907869775213165
4	1.6265576254184642	3.903876489840801
5	0.8764702426280805	2.6295038066669663
6	0.5411083700825976	1.9480590894018275
7	0.3791852027124881	1.592634227220779
8	0.26276221755630097	1.261303291695641
9	0.20969208944361406	1.1323773667202899
>10	1.1009967584725469	11.953926817566183
>50	0.0744169942671262	3.0656700552734457
>100	0.030896244438444784	3.138277076428117
>500	7.45579719118731E-4	0.2913183959596855
>1k	1.4337697925479142E-4	0.145286168252385
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.442305242975116E-5	0.0	0.0	0.0
6	0.0	3.442305242975116E-5	0.0	0.0	0.0
7	0.0	3.442305242975116E-5	0.0	0.0	0.0
8	0.0	3.442305242975116E-5	0.0	0.0	0.0
9	0.0	3.442305242975116E-5	0.0	0.0	0.0
10-11	0.0	3.442305242975116E-5	0.0	0.0	0.0
12-13	0.0	5.1634578644626735E-5	0.0	8.60576310743779E-6	0.0
14-15	0.0	7.74518679669401E-5	0.0	1.721152621487558E-5	0.0
16-17	0.0	8.605763107437789E-5	1.721152621487558E-5	1.721152621487558E-5	0.0
18-19	0.0	8.605763107437789E-5	1.721152621487558E-5	2.5817289322313368E-5	0.0
20-21	0.0	8.605763107437789E-5	1.721152621487558E-5	8.605763107437789E-5	0.0
22-23	0.0	8.605763107437789E-5	1.721152621487558E-5	2.0653831457850694E-4	0.0
24-25	0.0	1.0326915728925347E-4	1.721152621487558E-5	6.884610485950231E-4	0.0
26-27	0.0	1.2048068350412904E-4	1.721152621487558E-5	0.0015834604117685534	0.0
28-29	0.0	1.2048068350412904E-4	1.721152621487558E-5	0.003915622213884194	0.0
30-31	0.0	1.2048068350412904E-4	1.721152621487558E-5	0.010051531309487338	0.0
32-33	0.0	1.2048068350412904E-4	1.721152621487558E-5	0.023381858362908473	0.0
34-35	1.721152621487558E-5	1.2048068350412904E-4	1.721152621487558E-5	0.04340746911391621	0.0
36-37	3.442305242975116E-5	1.376922097190046E-4	1.721152621487558E-5	0.07639335910472525	0.0
38-39	3.442305242975116E-5	1.549037359338802E-4	1.721152621487558E-5	0.12444794029665787	0.0
40-41	3.442305242975116E-5	1.549037359338802E-4	1.721152621487558E-5	0.1810222269649539	0.0
42-43	3.442305242975116E-5	2.0653831457850694E-4	1.721152621487558E-5	0.24201987587047294	0.0
44-45	3.442305242975116E-5	2.151440776859447E-4	1.721152621487558E-5	0.3123892007999918	0.0
46-47	3.442305242975116E-5	2.237498407933825E-4	1.721152621487558E-5	0.401656781513444	0.0
48-49	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	0.5019053159519867	0.0
50-51	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	0.6076959618317195	0.0
52-53	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	0.7153454525426588	0.0
54-55	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	0.8356626265477465	0.0
56-57	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	0.9772618527175279	0.0
58-59	5.1634578644626735E-5	2.237498407933825E-4	1.721152621487558E-5	1.1329659246203998	0.0
60-61	5.1634578644626735E-5	2.581728932231337E-4	1.721152621487558E-5	1.3024134002058498	0.0
62-63	5.1634578644626735E-5	2.581728932231337E-4	1.721152621487558E-5	1.4727816924437958	0.0
64-65	5.1634578644626735E-5	2.581728932231337E-4	1.721152621487558E-5	1.6626076150676585	0.0
66-67	5.1634578644626735E-5	2.581728932231337E-4	1.721152621487558E-5	1.8784573653284131	0.0
68-69	5.1634578644626735E-5	2.7538441943800927E-4	1.721152621487558E-5	2.1145564761809688	0.0
70-71	5.1634578644626735E-5	2.925959456528848E-4	1.721152621487558E-5	2.37240235040602	0.0
72-73	5.1634578644626735E-5	3.098074718677604E-4	1.721152621487558E-5	2.644826387335071	0.0
74-75	6.884610485950232E-5	3.2701899808263595E-4	1.721152621487558E-5	2.9222245553402204	0.0
76-77	6.884610485950232E-5	3.6144205051238715E-4	1.721152621487558E-5	3.2167137688767413	0.0
78-79	6.884610485950232E-5	3.786535767272627E-4	1.721152621487558E-5	3.546529639969295	0.0
80-81	6.884610485950232E-5	3.958651029421383E-4	1.721152621487558E-5	3.900682609129682	0.0
82-83	6.884610485950232E-5	4.044708660495761E-4	1.721152621487558E-5	4.274439506648813	1.721152621487558E-5
84-85	6.884610485950232E-5	4.130766291570139E-4	1.721152621487558E-5	4.647955442800935	1.721152621487558E-5
86-87	6.884610485950232E-5	4.130766291570139E-4	1.721152621487558E-5	5.041032278496264	1.721152621487558E-5
88	6.884610485950232E-5	4.130766291570139E-4	1.721152621487558E-5	5.359841378574404	1.721152621487558E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5510	0.0	37.72569	1
TATAACG	585	0.0	36.153175	2
GTATCAA	8440	0.0	31.984234	1
TCAACGC	8885	0.0	29.358173	4
TACCTGG	5055	0.0	29.101336	2
ATCAACG	9000	0.0	28.983044	3
CAACGCA	9710	0.0	26.912886	5
GTACCTG	5710	0.0	26.685503	1
AACGCAG	10450	0.0	25.367125	6
TATCAAC	10545	0.0	25.048372	2
ATAACGC	940	0.0	23.499765	3
TAACGCA	950	0.0	23.252398	4
GTACATG	18485	0.0	22.872135	1
ACGCAGA	12125	0.0	21.863178	7
TACATGG	18260	0.0	21.620626	2
TATCACG	850	0.0	21.564302	2
CGCAGAG	12465	0.0	21.114183	8
ACATGGG	18790	0.0	20.360624	3
ACCTGGG	7190	0.0	20.06795	3
GTATAAC	1725	0.0	19.62953	1
>>END_MODULE