FastQCFastQC Report
Wed 25 May 2016
SRR2049431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810060
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157890.27175278740667047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146930.25288895467516687No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99250.17082439768264013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79960.13762336361414512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.050.904981
TATAACG6850.044.603922
GTATCAA149100.037.462961
ATCAACG177450.030.9927483
TCAACGC178750.030.7936464
TAACGCA10700.030.7513734
TACCTGG48200.030.4269082
CAACGCA186550.029.5061075
ATAACGC10300.029.2074093
TATCAAC192000.028.61962
AACGCAG196950.028.1353326
GTACCTG59850.025.8678741
TATCACG10150.025.4710542
ACGCAGA226400.024.5165987
CGCAGAG231650.023.9000998
GTACATG181200.023.424881
GTATAAC18400.023.0172621
TACATGG183100.022.1037062
ACCTGGG67050.021.2419383
GTGGTAT43750.021.1893271
Produced by FastQC (version 0.11.5)