##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049431_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5810060
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72392109547922	31.0	31.0	34.0	30.0	34.0
2	31.88691562565619	33.0	31.0	34.0	30.0	34.0
3	32.02786305132821	33.0	31.0	34.0	30.0	34.0
4	35.78469671569657	37.0	35.0	37.0	33.0	37.0
5	35.8019734735958	37.0	35.0	37.0	35.0	37.0
6	35.903410291804214	37.0	35.0	37.0	35.0	37.0
7	35.889134019270024	37.0	35.0	37.0	35.0	37.0
8	35.90914173003377	37.0	35.0	37.0	35.0	37.0
9	37.63377262885409	39.0	37.0	39.0	35.0	39.0
10-11	37.59915043906604	39.0	37.0	39.0	35.0	39.0
12-13	37.57450112391266	39.0	37.0	39.0	35.0	39.0
14-15	38.88562906407162	40.0	38.0	41.0	35.0	41.0
16-17	38.88173865674365	40.0	38.0	41.0	35.0	41.0
18-19	38.93622733672286	40.0	38.0	41.0	35.0	41.0
20-21	38.939604410281476	40.0	38.0	41.0	35.0	41.0
22-23	38.90256486163654	40.0	38.0	41.0	35.0	41.0
24-25	38.8774498025838	40.0	38.0	41.0	35.0	41.0
26-27	38.78241197164917	40.0	38.0	41.0	35.0	41.0
28-29	38.70035188965346	40.0	38.0	41.0	35.0	41.0
30-31	38.60696808638809	40.0	38.0	41.0	34.5	41.0
32-33	38.49299869536631	40.0	38.0	41.0	34.0	41.0
34-35	38.37892792845513	40.0	38.0	41.0	34.0	41.0
36-37	38.2558985621491	40.0	38.0	41.0	34.0	41.0
38-39	38.16506094601432	40.0	38.0	41.0	34.0	41.0
40-41	38.08899064725665	40.0	38.0	41.0	33.5	41.0
42-43	37.95560751524081	40.0	37.5	41.0	33.0	41.0
44-45	37.79644125878218	40.0	37.0	41.0	33.0	41.0
46-47	37.573754573963086	40.0	37.0	41.0	33.0	41.0
48-49	37.34286255219395	40.0	37.0	41.0	32.5	41.0
50-51	37.171647280750975	40.0	36.5	41.0	32.0	41.0
52-53	37.03458888548484	40.0	36.0	41.0	32.0	41.0
54-55	36.861678364767315	39.0	36.0	41.0	31.5	41.0
56-57	36.67897896063035	39.0	35.0	41.0	31.0	41.0
58-59	36.46365915326176	39.0	35.0	41.0	31.0	41.0
60-61	36.22766520483437	39.0	35.0	41.0	31.0	41.0
62-63	35.96640705947959	38.0	35.0	40.0	31.0	41.0
64-65	35.71077536204446	37.5	35.0	40.0	30.0	41.0
66-67	35.41211906589605	37.0	35.0	40.0	30.0	41.0
68-69	35.088574042264625	36.5	34.5	39.0	30.0	41.0
70-71	34.78670125609719	36.0	34.0	39.0	29.5	41.0
72-73	34.44521476542411	35.5	34.0	39.0	29.0	40.5
74-75	34.12745427758061	35.0	34.0	37.5	29.0	40.0
76-77	33.82804644014003	35.0	34.0	37.0	29.0	39.0
78-79	33.548579532741485	35.0	34.0	37.0	29.0	39.0
80-81	33.275625294747385	35.0	34.0	36.0	29.0	39.0
82-83	33.043560651697234	35.0	34.0	36.0	29.0	37.0
84-85	32.80359204552104	35.0	33.5	35.5	29.0	37.0
86-87	32.59673936241622	35.0	33.0	35.0	28.0	37.0
88-89	32.410898252341624	35.0	33.0	35.0	27.5	36.0
90-91	32.25083630805878	35.0	33.0	35.0	27.0	36.0
92-93	32.110052822173955	35.0	33.0	35.0	27.0	36.0
94-95	31.960865894672345	35.0	33.0	35.0	27.0	35.5
96-97	31.82276396457179	35.0	33.0	35.0	27.0	35.0
98-99	31.691459038288762	35.0	33.0	35.0	26.5	35.0
100	31.58123823161895	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19017076358784735
1101	2	0.3149078129847105
1101	3	0.4099138840161558
1101	4	0.2461672010838143
1101	5	0.25483144464938334
1101	6	0.23284889099672057
1101	7	0.273292341153649
1101	8	0.24128326502862052
1101	9	0.30592735393015147
1101	10-11	0.32029014892670915
1101	12-13	0.2787899763136039
1101	14-15	0.4508960237001176
1101	16-17	0.4105925386738818
1101	18-19	0.3968535626918879
1101	20-21	0.43370994415024455
1101	22-23	0.43461464596499155
1101	24-25	0.47202679407214276
1101	26-27	0.4336359755090342
1101	28-29	0.4221532751850461
1101	30-31	0.43225318414567226
1101	32-33	0.42919991779767486
1101	34-35	0.438266031920584
1101	36-37	0.42216621498111806
1101	38-39	0.37673323471043574
1101	40-41	0.4238992184535846
1101	42-43	0.41891604299910057
1101	44-45	0.3998712635382802
1101	46-47	0.3928646774858393
1101	48-49	0.3910455540331981
1101	50-51	0.35133452917044394
1101	52-53	0.3811463980327119
1101	54-55	0.38428115304441235
1101	56-57	0.3502091665707141
1101	58-59	0.35966843932964565
1101	60-61	0.3105270383702887
1101	62-63	0.2915061136296444
1101	64-65	0.25682821287739444
1101	66-67	0.26439758883838493
1101	68-69	0.2540653177661554
1101	70-71	0.2549142201889367
1101	72-73	0.2613119632169614
1101	74-75	0.23099970455778163
1101	76-77	0.24531396863632438
1101	78-79	0.2305506487512048
1101	80-81	0.2364283738409867
1101	82-83	0.21387644667368733
1101	84-85	0.22969515905879234
1101	86-87	0.1610232382155985
1101	88-89	0.2287271074889219
1101	90-91	0.25845989387558177
1101	92-93	0.24337484356916406
1101	94-95	0.2563325209399636
1101	96-97	0.23657390878607032
1101	98-99	0.22401423256472341
1101	100	0.21224969468436328
1102	1	0.1082662309688942
1102	2	0.14486459574616006
1102	3	0.2854669149860172
1102	4	0.18935879904640274
1102	5	0.13814789711272368
1102	6	0.1412505454229347
1102	7	0.1604452675741257
1102	8	0.16539240513718312
1102	9	0.15985035706840733
1102	10-11	0.16827134061373528
1102	12-13	0.17856077016990213
1102	14-15	0.2601427776319625
1102	16-17	0.2528652176768418
1102	18-19	0.2277843465739835
1102	20-21	0.24707319786217852
1102	22-23	0.24035094169751403
1102	24-25	0.2021645304017241
1102	26-27	0.15171615243855996
1102	28-29	0.1855246604622991
1102	30-31	0.20780155843431913
1102	32-33	0.2242592976139477
1102	34-35	0.21617920225052245
1102	36-37	0.23198120901067654
1102	38-39	0.17519220012695058
1102	40-41	0.18607535416741428
1102	42-43	0.125783288917809
1102	44-45	0.1213328784718044
1102	46-47	0.10317724254978344
1102	48-49	0.10922540252889235
1102	50-51	0.09043684954853859
1102	52-53	0.12130581388938566
1102	54-55	0.0673297229505323
1102	56-57	0.08115268021039412
1102	58-59	0.08228184574178954
1102	60-61	0.03896843515095583
1102	62-63	0.05795609238854382
1102	64-65	-7.281617690466646E-4
1102	66-67	0.016008394319406705
1102	68-69	0.0439400054492296
1102	70-71	0.053641008872510554
1102	72-73	0.07341154331603406
1102	74-75	0.09565188708705108
1102	76-77	0.012067088368354462
1102	78-79	0.06119425994242533
1102	80-81	0.02612233870682701
1102	82-83	0.03666100294589114
1102	84-85	-0.008502185161880504
1102	86-87	0.026719015693799975
1102	88-89	-0.021704336589110085
1102	90-91	0.03127677850810073
1102	92-93	0.029342686284998365
1102	94-95	0.03579838838808769
1102	96-97	0.011934862071846908
1102	98-99	0.05663563529651228
1102	100	0.09506929560742705
1103	1	0.19829167049439533
1103	2	0.27409520480681593
1103	3	0.35527334456619997
1103	4	0.26325691226496417
1103	5	0.23350743713440636
1103	6	0.24560987614859187
1103	7	0.23291973783481978
1103	8	0.18766578690496516
1103	9	0.2795990728460893
1103	10-11	0.2663039963223852
1103	12-13	0.23516713101867737
1103	14-15	0.3410480773974669
1103	16-17	0.3743440115973442
1103	18-19	0.3725044357835401
1103	20-21	0.35991377033519
1103	22-23	0.35298500517149023
1103	24-25	0.40645187434911634
1103	26-27	0.36103062425323174
1103	28-29	0.39812269215828167
1103	30-31	0.3796751049363465
1103	32-33	0.3646729175492496
1103	34-35	0.35845225540972336
1103	36-37	0.31766722626999666
1103	38-39	0.34966158555364046
1103	40-41	0.3159586581946954
1103	42-43	0.33696656851442697
1103	44-45	0.3664444802436435
1103	46-47	0.34042436557393074
1103	48-49	0.29371553022096464
1103	50-51	0.272486413550979
1103	52-53	0.27390714242011427
1103	54-55	0.23145807255075823
1103	56-57	0.2316446871136364
1103	58-59	0.2569648421054467
1103	60-61	0.21110978997344887
1103	62-63	0.2602621415351791
1103	64-65	0.2731126138577693
1103	66-67	0.26328056683829715
1103	68-69	0.23474400931453232
1103	70-71	0.2408198611594159
1103	72-73	0.26975042905959157
1103	74-75	0.2993307753827068
1103	76-77	0.25619204285837327
1103	78-79	0.27041397271088385
1103	80-81	0.25300771127908916
1103	82-83	0.2904053595924623
1103	84-85	0.24400091397026813
1103	86-87	0.19625580964408584
1103	88-89	0.24112779801388484
1103	90-91	0.24039231160566743
1103	92-93	0.235198967323349
1103	94-95	0.20450192155998081
1103	96-97	0.24360550505596734
1103	98-99	0.33600838292650437
1103	100	0.2969900435082842
1104	1	0.13767815894231106
1104	2	0.16350569379008917
1104	3	0.25509353715509775
1104	4	0.18904862204232842
1104	5	0.15774966938154478
1104	6	0.11119797532548148
1104	7	0.1221859416831208
1104	8	0.11935219138439379
1104	9	0.19956064824864939
1104	10-11	0.2135331994319003
1104	12-13	0.19925721100018734
1104	14-15	0.2826540923248402
1104	16-17	0.2853868545189897
1104	18-19	0.2518259083762615
1104	20-21	0.29318737807044215
1104	22-23	0.2648032694082616
1104	24-25	0.2455757145890871
1104	26-27	0.25189920325684056
1104	28-29	0.24236109470638922
1104	30-31	0.229027832383899
1104	32-33	0.2190461415281746
1104	34-35	0.24239958450996113
1104	36-37	0.21218330054509238
1104	38-39	0.2290780290437695
1104	40-41	0.20674315742482463
1104	42-43	0.29201914489584624
1104	44-45	0.2353900577128485
1104	46-47	0.23153962705067244
1104	48-49	0.2941194736981174
1104	50-51	0.2869046368644206
1104	52-53	0.3134587189399838
1104	54-55	0.27365542060659465
1104	56-57	0.26098137611996464
1104	58-59	0.29190402764226775
1104	60-61	0.21059318123202786
1104	62-63	0.19377985734303138
1104	64-65	0.16239902712132448
1104	66-67	0.19420870278089097
1104	68-69	0.21278611612084575
1104	70-71	0.1746462898812382
1104	72-73	0.1689569498455441
1104	74-75	0.18526635037840578
1104	76-77	0.1291141640516571
1104	78-79	0.13120723232189135
1104	80-81	0.1097704686208445
1104	82-83	0.1245251067581421
1104	84-85	0.14420990781292176
1104	86-87	0.12393691427113396
1104	88-89	0.13397776773685166
1104	90-91	0.08566543027900053
1104	92-93	0.13204411735303268
1104	94-95	0.13799843380365928
1104	96-97	0.10458429214384424
1104	98-99	0.12983600907843496
1104	100	0.1072391994832742
1105	1	0.10065651304844181
1105	2	0.11241496655182104
1105	3	0.23849313985372333
1105	4	0.14133996913732716
1105	5	0.1070910345915621
1105	6	0.10538788586474368
1105	7	0.12729111007835314
1105	8	0.12868793556875602
1105	9	0.16020927108083072
1105	10-11	0.1189983423236427
1105	12-13	0.1610976531369417
1105	14-15	0.19518576809530686
1105	16-17	0.2483788148365207
1105	18-19	0.24289682675326674
1105	20-21	0.2363342513939628
1105	22-23	0.1550193137636171
1105	24-25	0.23941484650446654
1105	26-27	0.20700912771656022
1105	28-29	0.22506647874246966
1105	30-31	0.18425716518488144
1105	32-33	0.17656787297658383
1105	34-35	0.19376506234163315
1105	36-37	0.15751204091596094
1105	38-39	0.09366764033551789
1105	40-41	0.1470290175293627
1105	42-43	0.13488770830432628
1105	44-45	0.14685152193928275
1105	46-47	0.15012236525780764
1105	48-49	0.15716840616252625
1105	50-51	0.1376921949997083
1105	52-53	0.10597379901386716
1105	54-55	0.06156958149860259
1105	56-57	0.11713157845863265
1105	58-59	0.08845581835265648
1105	60-61	0.07230416957529684
1105	62-63	0.09698038183408642
1105	64-65	0.03455584824573066
1105	66-67	0.019989050630407235
1105	68-69	0.13727150627705242
1105	70-71	0.14043634277612682
1105	72-73	0.11811566262864659
1105	74-75	0.09779238384779632
1105	76-77	0.07740856256057782
1105	78-79	0.046183045106864995
1105	80-81	0.04600022582350505
1105	82-83	0.040391396843972416
1105	84-85	0.07307762650064831
1105	86-87	0.07845398023374628
1105	88-89	0.06002853346755188
1105	90-91	0.09102288500291422
1105	92-93	0.059228726846214386
1105	94-95	0.04988413438274364
1105	96-97	0.07465544278240088
1105	98-99	0.0821799409819981
1105	100	0.14197817880064179
1106	1	0.11946180854672406
1106	2	0.12679547853347017
1106	3	0.1984382709177126
1106	4	0.11064353814523287
1106	5	0.10537978374522083
1106	6	0.14214216937944002
1106	7	0.11990146559242731
1106	8	0.10632539389118278
1106	9	0.09737807818550692
1106	10-11	0.13348960961600653
1106	12-13	0.11391992112185534
1106	14-15	0.1608621833512771
1106	16-17	0.19765155286185632
1106	18-19	0.18294277105445644
1106	20-21	0.12992006522325283
1106	22-23	0.12164323257310627
1106	24-25	0.171635710396437
1106	26-27	0.14900068925869192
1106	28-29	0.174091395215072
1106	30-31	0.14279804071512814
1106	32-33	0.14365399334646867
1106	34-35	0.131878128053863
1106	36-37	0.10608141795716364
1106	38-39	0.08439481798293258
1106	40-41	0.0982086952443666
1106	42-43	0.08482016244607138
1106	44-45	0.07973529866727347
1106	46-47	-0.010025763011888955
1106	48-49	0.04587300141112394
1106	50-51	0.03642144109004164
1106	52-53	0.048095854232300894
1106	54-55	0.13919974661550327
1106	56-57	0.14682791159253128
1106	58-59	0.09313009756610313
1106	60-61	0.06718162622330226
1106	62-63	0.08734143863541277
1106	64-65	0.04251253153780965
1106	66-67	0.09876570131650197
1106	68-69	0.11587423612677128
1106	70-71	0.10381140928373611
1106	72-73	0.12574404144069717
1106	74-75	0.08276931744908467
1106	76-77	0.06784946156741967
1106	78-79	0.03670971127363032
1106	80-81	0.008447086216342825
1106	82-83	0.06539462869992718
1106	84-85	0.05537369735472453
1106	86-87	0.06695091547528875
1106	88-89	-0.013183944299058226
1106	90-91	0.03487683195539404
1106	92-93	0.013447554813616591
1106	94-95	-0.034694679484921664
1106	96-97	-0.013507121101351771
1106	98-99	-0.022203046803856097
1106	100	-0.057210962251552644
1107	1	0.0848101033584463
1107	2	0.09690336872565908
1107	3	0.2094078493068352
1107	4	0.14344233747893753
1107	5	0.1462731306790559
1107	6	0.10246785776562461
1107	7	0.11500792940196902
1107	8	0.10666320305092825
1107	9	0.1269616022830249
1107	10-11	0.12357833757491932
1107	12-13	0.11248762420047598
1107	14-15	0.20140937156838135
1107	16-17	0.22399388330670433
1107	18-19	0.19430129963986076
1107	20-21	0.16462012826240624
1107	22-23	0.2253869999767204
1107	24-25	0.23468878157223827
1107	26-27	0.22684204350117199
1107	28-29	0.21499674463707663
1107	30-31	0.23219257259025028
1107	32-33	0.2702485847817755
1107	34-35	0.20346009774219453
1107	36-37	0.2305021674412302
1107	38-39	0.21210598871606834
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2216	70-71	-0.6741579064499277
2216	72-73	-0.6488566742476323
2216	74-75	-0.6428463749339741
2216	76-77	-0.5087033234445784
2216	78-79	-0.544461730169715
2216	80-81	-0.5466376184151969
2216	82-83	-0.6112159127142434
2216	84-85	-0.7236609570575752
2216	86-87	-0.6432905251286876
2216	88-89	-0.6748897366695026
2216	90-91	-0.5409572098907347
2216	92-93	-0.5452181609517979
2216	94-95	-0.6269009340778844
2216	96-97	-0.6964297549303282
2216	98-99	-0.7253900981782202
2216	100	-0.7467184825747815
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	420.0
3	30.0
4	56.0
5	120.0
6	336.0
7	943.0
8	2025.0
9	3527.0
10	4441.0
11	5806.0
12	8544.0
13	11544.0
14	12089.0
15	14798.0
16	19681.0
17	27978.0
18	28067.0
19	16844.0
20	13512.0
21	13609.0
22	14599.0
23	15924.0
24	18921.0
25	23315.0
26	29794.0
27	39075.0
28	51092.0
29	67804.0
30	87772.0
31	114539.0
32	149124.0
33	195072.0
34	272493.0
35	412199.0
36	711021.0
37	1340545.0
38	1589082.0
39	492084.0
40	1235.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.37636398728898	19.242801800497688	13.524257087908678	23.85657712430465
2	16.642186139213706	20.097382815323765	37.61117785358499	25.649253191877534
3	18.097937026467886	24.94549109647749	30.163354595305385	26.793217281749243
4	13.377262885409099	17.205794776646023	36.61831375235368	32.7986285855912
5	13.683486827586897	36.19422729405657	34.207030129766935	15.9152557485896
6	31.144000481923563	36.53453928967909	17.74855638075404	14.572903847643307
7	27.751365734605148	31.562634465048557	21.9574668764178	18.728532923928494
8	26.29404859846542	34.05419221144016	20.168087765014477	19.483671425079947
9	25.48119984991549	16.31463358381841	21.015824277201954	37.18834228906414
10-11	24.670967253350224	25.846290743985435	28.011328626554633	21.471413376109712
12-13	25.48093307125916	23.42442246723786	28.173822645549272	22.92082181595371
14-15	22.83713937549698	25.18052309270472	25.894353930940472	26.08798360085782
16-17	22.299847161647214	28.240603367262988	26.32102766580724	23.13852180528256
18-19	22.208238813368535	26.85256262413813	28.83453010812281	22.104668454370525
20-21	23.139545891092347	26.12335328722939	28.691751892407307	22.045348929270954
22-23	22.841132449578833	26.059352226999376	28.65465244765114	22.444862875770646
24-25	22.65418945759596	26.163869219939208	28.65395538083944	22.527985941625385
26-27	22.547211216407405	26.078870097727048	28.7191698536676	22.654748832197946
28-29	22.572185140945187	26.039318010485264	28.82045107967904	22.56804576889051
30-31	22.77684739916627	26.404529385238707	28.484516511017098	22.334106704577923
32-33	22.43037077069772	26.229815182631505	28.752654877918644	22.58715916875213
34-35	22.604370694967006	25.88666037872242	28.76543443613319	22.74353449017738
36-37	22.998695366312912	26.101047149254914	28.427795926375975	22.4724615580562
38-39	22.825986306509744	26.271458470308396	28.408355507516276	22.494199715665587
40-41	22.679060112976458	26.053113048746486	28.564722223178418	22.703104615098642
42-43	22.58335542145864	26.19453155389101	28.448596055806654	22.773516968843698
44-45	22.71183085888958	26.358686485165382	28.195973879787818	22.733508776157215
46-47	22.887078618809443	26.424176342412984	27.885090687531626	22.803654351245942
48-49	22.869454015965413	26.358634850586743	27.873567570730767	22.89834356271708
50-51	22.841795093338106	26.37458993538793	27.69920620441097	23.084408766862992
52-53	22.804076033638207	26.469304964148392	27.615549581243563	23.111069420969834
54-55	22.871725937425776	26.777391283394664	27.580240135213753	22.77064264396581
56-57	22.639783410154113	26.514881085565385	27.604671896675764	23.24066360760474
58-59	22.60909525891299	26.135341115238052	27.8932747682468	23.36228885760216
60-61	22.85158845175437	26.455578771992027	27.658251377782673	23.034581398470927
62-63	22.641719706853287	26.503082584345083	27.75550510665983	23.099692602141804
64-65	22.678638430584193	26.21194101265736	27.815263525677874	23.294157031080573
66-67	22.511755472404758	26.290700956616625	27.77615033235457	23.42139323862404
68-69	22.864910173044684	26.421491344323467	27.578114511726216	23.135483970905636
70-71	22.851579845991264	26.154067255759838	27.624224190455866	23.370128707793032
72-73	22.53969838521461	26.353032498803795	27.775935188276886	23.331333927704705
74-75	22.562142215398808	26.237973446057357	27.694533275043632	23.505351063500203
76-77	22.655222149168853	26.33855760525709	27.417857991139506	23.58836225443455
78-79	22.657296138077747	26.45052718904796	27.55400976926228	23.338166903612013
80-81	22.493319549170796	26.247822845571285	27.653740299458722	23.605117305799205
82-83	22.378894894695385	26.290564473426148	27.686720135737787	23.64382049614068
84-85	22.423374333114573	26.25883579622897	27.456600489739213	23.861189380917246
86-87	22.54590514600284	26.318273384068636	27.473457940965478	23.66236352896305
88-89	22.290719722314474	26.21996237365912	27.571582947441453	23.917734956584958
90-91	22.10543156038052	26.157684088794596	27.603790385757147	24.133093965067733
92-93	22.288988761878993	26.309722849010146	27.42597907142215	23.975309317688705
94-95	22.18262221272526	26.015889554011085	27.60645423739082	24.195033995872837
96-97	22.08643369134449	26.13443563695065	27.547719545278245	24.23141112642662
98-99	21.970869649741992	26.130000085198102	27.60214585622674	24.29698440883317
100	22.055224290591546	26.053467335576343	27.457413462048187	24.43389491178392
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1787.0
1	1297.5
2	4389.0
3	6352.0
4	2983.0
5	1240.0
6	1862.5
7	4055.0
8	7001.5
9	8110.0
10	5963.0
11	3464.0
12	2969.5
13	3244.0
14	3671.5
15	4102.5
16	4446.5
17	4842.0
18	5435.5
19	6004.5
20	6779.0
21	8036.0
22	9901.0
23	12953.0
24	17027.5
25	22271.0
26	29320.5
27	38019.0
28	49168.5
29	60921.5
30	70389.5
31	80151.5
32	90794.5
33	99883.0
34	108566.5
35	118691.5
36	129240.5
37	140048.0
38	151418.5
39	160501.0
40	169248.0
41	177874.5
42	182756.0
43	185891.0
44	189477.0
45	192307.0
46	195341.5
47	196074.5
48	196290.5
49	198922.5
50	200962.5
51	200610.5
52	195492.5
53	191842.5
54	189775.5
55	182640.5
56	171308.5
57	158129.5
58	145788.5
59	135073.5
60	122617.5
61	107435.5
62	91431.0
63	76322.0
64	62631.5
65	51211.0
66	40678.0
67	30898.0
68	23187.0
69	17141.0
70	12533.5
71	8966.0
72	6525.0
73	4818.5
74	3735.5
75	2772.5
76	1816.5
77	1221.5
78	840.0
79	501.5
80	275.5
81	134.0
82	58.5
83	27.5
84	17.0
85	10.5
86	9.0
87	9.0
88	5.5
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	2.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12426721927140166
2	0.0
3	0.0
4	0.0
5	0.012426721927140167
6	1.7211526214875578E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.8072102525619355E-4
82-83	5.937976544132075E-4
84-85	4.991342602313917E-4
86-87	6.196149437355208E-4
88-89	5.937976544132075E-4
90-91	7.659129165619633E-4
92-93	2.409613670082581E-4
94-95	2.8399018254544704E-4
96-97	7.831244427768388E-4
98-99	0.0012306241243636039
100	1.376922097190046E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5810060.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.37229219872656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9858488675198	44.16854616394348
2	13.53309552221414	15.528494213074953
3	4.007258933941149	6.897168914220872
4	1.7166205396924539	3.939458207902846
5	0.9671162591078055	2.7742838303836184
6	0.5658328889802038	1.9477877905333112
7	0.4072526557960735	1.6355512856927819
8	0.28188925193436476	1.2938106023727058
9	0.21886573875940205	1.130114620475511
>10	1.1973944897254463	12.40804587465862
>50	0.08099340036393551	3.1906509604689672
>100	0.03639221137761064	3.5237116648476516
>500	0.001079435835663281	0.4173872691648407
>1k	2.3986983465190415E-4	0.3123431198014223
>5k	5.996745863991523E-5	0.30828336448437976
>10k+	5.996745863991523E-5	0.5243621179740453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15789	0.27175278740667047	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14693	0.25288895467516687	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9925	0.17082439768264013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7996	0.13762336361414512	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.721152621487558E-5	0.0
8	0.0	0.0	0.0	1.721152621487558E-5	0.0
9	0.0	0.0	0.0	1.721152621487558E-5	0.0
10-11	0.0	8.60576310743779E-6	0.0	1.721152621487558E-5	0.0
12-13	0.0	1.721152621487558E-5	0.0	1.721152621487558E-5	0.0
14-15	0.0	1.721152621487558E-5	0.0	1.721152621487558E-5	0.0
16-17	3.442305242975116E-5	1.721152621487558E-5	0.0	3.442305242975116E-5	0.0
18-19	3.442305242975116E-5	1.721152621487558E-5	0.0	4.302881553718895E-5	0.0
20-21	3.442305242975116E-5	1.721152621487558E-5	0.0	1.0326915728925347E-4	0.0
22-23	3.442305242975116E-5	1.721152621487558E-5	0.0	2.237498407933825E-4	0.0
24-25	3.442305242975116E-5	5.1634578644626735E-5	0.0	7.056725748098986E-4	0.0
26-27	3.442305242975116E-5	7.74518679669401E-5	0.0	0.0015834604117685531	0.0
28-29	3.442305242975116E-5	8.605763107437789E-5	0.0	0.003881199161454443	0.0
30-31	3.442305242975116E-5	1.4629797282644243E-4	0.0	0.009991290967735274	0.0
32-33	3.442305242975116E-5	1.549037359338802E-4	0.0	0.023467915993982853	0.0
34-35	3.442305242975116E-5	1.549037359338802E-4	1.721152621487558E-5	0.043536555560527775	0.0
36-37	3.442305242975116E-5	1.6350949904131798E-4	1.721152621487558E-5	0.07652244555133682	0.0
38-39	3.442305242975116E-5	1.7211526214875578E-4	1.721152621487558E-5	0.12487822845202975	0.0
40-41	3.442305242975116E-5	1.7211526214875578E-4	1.721152621487558E-5	0.18188280327569767	0.0
42-43	3.442305242975116E-5	2.151440776859447E-4	1.721152621487558E-5	0.24331074033658862	0.0
44-45	3.442305242975116E-5	2.237498407933825E-4	1.721152621487558E-5	0.3142050168156611	0.0
46-47	3.442305242975116E-5	2.4096136700825808E-4	1.721152621487558E-5	0.40395452026312983	0.0
48-49	3.442305242975116E-5	2.4096136700825808E-4	1.721152621487558E-5	0.5046591601463668	0.0
50-51	3.442305242975116E-5	2.4096136700825808E-4	1.721152621487558E-5	0.6106993731562153	0.0
52-53	3.442305242975116E-5	2.925959456528848E-4	1.721152621487558E-5	0.7188307866011712	0.0
54-55	3.442305242975116E-5	2.925959456528848E-4	1.721152621487558E-5	0.8399913253907878	0.0
56-57	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	0.9824167048188831	0.0
58-59	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	1.1390501991373583	0.0
60-61	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	1.3090656550878992	0.0
62-63	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	1.4797867836132501	0.0
64-65	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	1.669638523526435	0.0
66-67	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	1.8862111578882146	0.0
68-69	3.442305242975116E-5	3.098074718677604E-4	1.721152621487558E-5	2.12271473960682	0.0
70-71	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	2.3812576806435732	0.0
72-73	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	2.655032822380492	0.0
74-75	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	2.932947336172088	0.0
76-77	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	3.228245491440708	0.0
78-79	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	3.5601267456790464	0.0
80-81	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	3.9156480311735162	0.0
82-83	3.442305242975116E-5	3.2701899808263595E-4	1.721152621487558E-5	4.291160504366564	0.0
84-85	3.442305242975116E-5	3.356247611900737E-4	1.721152621487558E-5	4.666122208720736	0.0
86-87	3.442305242975116E-5	3.4423052429751155E-4	1.721152621487558E-5	5.060946014326874	0.0
88	3.442305242975116E-5	3.4423052429751155E-4	1.721152621487558E-5	5.381338574816783	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8745	0.0	50.90498	1
TATAACG	685	0.0	44.60392	2
GTATCAA	14910	0.0	37.46296	1
ATCAACG	17745	0.0	30.992748	3
TCAACGC	17875	0.0	30.793646	4
TAACGCA	1070	0.0	30.751373	4
TACCTGG	4820	0.0	30.426908	2
CAACGCA	18655	0.0	29.506107	5
ATAACGC	1030	0.0	29.207409	3
TATCAAC	19200	0.0	28.6196	2
AACGCAG	19695	0.0	28.135332	6
GTACCTG	5985	0.0	25.867874	1
TATCACG	1015	0.0	25.471054	2
ACGCAGA	22640	0.0	24.516598	7
CGCAGAG	23165	0.0	23.900099	8
GTACATG	18120	0.0	23.42488	1
GTATAAC	1840	0.0	23.017262	1
TACATGG	18310	0.0	22.103706	2
ACCTGGG	6705	0.0	21.241938	3
GTGGTAT	4375	0.0	21.189327	1
>>END_MODULE