FastQCFastQC Report
Wed 25 May 2016
SRR2049430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12377698
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185600.035.646571
GGTATCA117550.034.36151
TCAACGC199850.031.5142784
ATCAACG202250.031.1641863
CAACGCA218000.029.0416535
AACGCAG230550.027.4806026
TATCAAC235550.027.21732
ACGCAGA273050.023.1343257
GTACATG400250.022.8501911
TACCTGG97850.022.6242312
CGCAGAG285500.022.025388
TACATGG405400.021.7965562
GTACCTG110450.021.6271631
ACATGGG425700.020.1168633
GCAGAGT336050.018.0413789
TATAACG11100.017.7844352
AGAGTAC288650.017.25927710-11
CATGGGG325350.016.6276654
ACCTGGG131400.016.5973263
GAGTACA292300.015.9260451
Produced by FastQC (version 0.11.5)