##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12377698 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.52971053260469 31.0 31.0 34.0 27.0 34.0 2 30.659573048235625 31.0 31.0 34.0 27.0 34.0 3 30.741099920195175 31.0 31.0 34.0 27.0 34.0 4 34.32827057179776 37.0 35.0 37.0 30.0 37.0 5 34.331608591516776 37.0 35.0 37.0 30.0 37.0 6 34.3622808538389 37.0 35.0 37.0 30.0 37.0 7 34.22861326880006 37.0 35.0 37.0 30.0 37.0 8 34.21998815934918 37.0 35.0 37.0 30.0 37.0 9 35.759029182970856 39.0 37.0 39.0 30.0 39.0 10-11 35.71335615071558 39.0 37.0 39.0 30.0 39.0 12-13 35.675778363634336 39.0 37.0 39.0 30.0 39.0 14-15 36.86272289887829 40.0 37.0 41.0 30.0 41.0 16-17 36.82779887665703 40.0 37.0 41.0 30.0 41.0 18-19 36.8204595070909 40.0 37.0 41.0 30.0 41.0 20-21 36.856168125931006 40.0 37.0 41.0 30.0 41.0 22-23 36.79522298895966 40.0 37.0 41.0 30.0 41.0 24-25 36.769331664094565 40.0 37.0 41.0 30.0 41.0 26-27 36.738250682800626 40.0 37.0 41.0 30.0 41.0 28-29 36.688909965326346 40.0 37.0 41.0 30.0 41.0 30-31 36.62336781039576 40.0 37.0 41.0 30.0 41.0 32-33 36.575946472437764 40.0 37.0 41.0 30.0 41.0 34-35 36.49591943510013 40.0 37.0 41.0 30.0 41.0 36-37 36.398438950441346 40.0 36.0 41.0 30.0 41.0 38-39 36.29119950252462 40.0 36.0 41.0 29.5 41.0 40-41 36.16844258924398 40.0 36.0 41.0 29.0 41.0 42-43 36.04967329143109 40.0 36.0 41.0 28.5 41.0 44-45 35.88805846612189 40.0 35.0 41.0 28.0 41.0 46-47 35.74484407359107 39.0 35.0 41.0 28.0 41.0 48-49 35.611156210145055 39.0 35.0 41.0 27.0 41.0 50-51 35.458508520728174 39.0 35.0 41.0 27.0 41.0 52-53 35.3044333041572 39.0 35.0 41.0 27.0 41.0 54-55 35.12843583677676 39.0 35.0 41.0 26.0 41.0 56-57 34.939069324522215 38.5 34.5 40.0 26.0 41.0 58-59 34.70944455907714 38.0 34.0 40.0 26.0 41.0 60-61 34.464866164936325 38.0 34.0 40.0 26.0 41.0 62-63 34.18792391767839 37.0 34.0 40.0 25.0 41.0 64-65 33.91606601647577 37.0 34.0 40.0 24.5 41.0 66-67 33.64207948844769 36.5 33.0 39.5 24.0 41.0 68-69 33.3293511038967 36.0 33.0 39.0 23.0 41.0 70-71 33.0097517729064 35.5 33.0 39.0 23.0 41.0 72-73 32.66506780986255 35.0 33.0 38.5 22.0 40.0 74-75 32.35015036721691 35.0 32.5 37.5 22.0 40.0 76-77 32.035686522647424 35.0 32.0 37.0 20.5 39.0 78-79 31.721566562700108 35.0 32.0 36.5 20.0 39.0 80-81 31.407607537362765 35.0 32.0 36.0 19.5 38.5 82-83 31.134189693430876 35.0 32.0 36.0 18.0 37.0 84-85 30.87746804777431 35.0 32.0 35.0 17.5 37.0 86-87 30.618657160644894 35.0 31.5 35.0 13.5 36.5 88-89 30.403832845170403 35.0 31.0 35.0 8.5 36.0 90-91 30.21101140939131 35.0 31.0 35.0 4.5 36.0 92-93 29.997071466762236 34.5 31.0 35.0 2.0 36.0 94-95 29.787550116346353 34.0 31.0 35.0 2.0 35.0 96-97 29.55862935902944 34.0 31.0 35.0 2.0 35.0 98-99 29.346820305358882 34.0 31.0 35.0 2.0 35.0 100 29.19685801026976 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.388783669388296 1101 2 0.3764330536718745 1101 3 0.3924272764941499 1101 4 0.4856148548177117 1101 5 0.463576441557187 1101 6 0.48843086749290876 1101 7 0.4798697195752766 1101 8 0.47690922145127956 1101 9 0.6144288497987347 1101 10-11 0.6548543874030273 1101 12-13 0.6698004844884409 1101 14-15 0.7757625995061801 1101 16-17 0.7620039874645883 1101 18-19 0.784432179859543 1101 20-21 0.8088853302190628 1101 22-23 0.7671994354216878 1101 24-25 0.7815354906414598 1101 26-27 0.7432619086454579 1101 28-29 0.7483470265923984 1101 30-31 0.7616465432709276 1101 32-33 0.7530147448176976 1101 34-35 0.7210720540968225 1101 36-37 0.7468159095211249 1101 38-39 0.7609753866059776 1101 40-41 0.8324799238898848 1101 42-43 0.7882440880767163 1101 44-45 0.7601463432307938 1101 46-47 0.6941413252466475 1101 48-49 0.6901973778550499 1101 50-51 0.7030567894049753 1101 52-53 0.6913078011478859 1101 54-55 0.6710173511820088 1101 56-57 0.6877894387394505 1101 58-59 0.7355396684133666 1101 60-61 0.7170638735729256 1101 62-63 0.704065825014986 1101 64-65 0.6981196341765639 1101 66-67 0.7048824937603086 1101 68-69 0.6453699106483697 1101 70-71 0.6585850640687667 1101 72-73 0.6734001807152197 1101 74-75 0.6753993880479072 1101 76-77 0.6683344695698281 1101 78-79 0.5949281279320893 1101 80-81 0.5936885190521117 1101 82-83 0.5977973931436509 1101 84-85 0.5782344861614668 1101 86-87 0.5943204810242904 1101 88-89 0.5520909077725626 1101 90-91 0.6209067649137907 1101 92-93 0.5636704568211286 1101 94-95 0.5520424563406863 1101 96-97 0.587063460778797 1101 98-99 0.6514759734430378 1101 100 0.6201227580657473 1102 1 0.2253899514099338 1102 2 0.2730246101618121 1102 3 0.28049851416244564 1102 4 0.329468569477136 1102 5 0.26442705617960627 1102 6 0.333129306881375 1102 7 0.36716813657762515 1102 8 0.39898407948150094 1102 9 0.501144826594178 1102 10-11 0.49984774055125314 1102 12-13 0.4932023825933882 1102 14-15 0.6114192742554536 1102 16-17 0.5567625887145624 1102 18-19 0.5645949597753273 1102 20-21 0.5769703861865523 1102 22-23 0.5832275133163876 1102 24-25 0.574399680264051 1102 26-27 0.5555840352293089 1102 28-29 0.5874628212840918 1102 30-31 0.5500988092773582 1102 32-33 0.5397332507948249 1102 34-35 0.6191109866911546 1102 36-37 0.5910785107697123 1102 38-39 0.6342136139936727 1102 40-41 0.6126007996823049 1102 42-43 0.5915218273757219 1102 44-45 0.54743026466938 1102 46-47 0.5049120962243876 1102 48-49 0.48320876990933215 1102 50-51 0.45594428734595027 1102 52-53 0.4668343888144122 1102 54-55 0.4746935180857079 1102 56-57 0.45646641102985797 1102 58-59 0.47254255998247174 1102 60-61 0.48069467605753147 1102 62-63 0.4223971841630245 1102 64-65 0.40568480673813667 1102 66-67 0.47294353015190893 1102 68-69 0.46251988051837856 1102 70-71 0.4297150881414993 1102 72-73 0.39639541208352114 1102 74-75 0.4475736009425475 1102 76-77 0.40803758688004876 1102 78-79 0.4356743983104501 1102 80-81 0.4127710511464109 1102 82-83 0.41141291144142755 1102 84-85 0.40646252457911203 1102 86-87 0.3944504501628181 1102 88-89 0.3321859080141074 1102 90-91 0.33437033906313474 1102 92-93 0.34125198464669637 1102 94-95 0.38936461252701093 1102 96-97 0.4290156392616993 1102 98-99 0.4636564621940238 1102 100 0.42003808436258083 1103 1 0.271039552487494 1103 2 0.333759688359347 1103 3 0.3159774303517615 1103 4 0.3242984150764272 1103 5 0.3281229733136186 1103 6 0.3399711945638373 1103 7 0.354933753684449 1103 8 0.3922614926947503 1103 9 0.4327527528415587 1103 10-11 0.48457433101265224 1103 12-13 0.4478800808877139 1103 14-15 0.5720004401855405 1103 16-17 0.5577343297578992 1103 18-19 0.5261796396174887 1103 20-21 0.5239673531123898 1103 22-23 0.541900195160764 1103 24-25 0.5519637514145188 1103 26-27 0.5576348729791576 1103 28-29 0.5385261209642493 1103 30-31 0.5621220587756142 1103 32-33 0.6259662228779135 1103 34-35 0.5921771985271818 1103 36-37 0.5628310241094638 1103 38-39 0.6090218466962796 1103 40-41 0.5991047440899209 1103 42-43 0.5850793840812614 1103 44-45 0.5257795411426898 1103 46-47 0.5383861770995537 1103 48-49 0.5221257382837265 1103 50-51 0.5452575748553343 1103 52-53 0.5876674156635247 1103 54-55 0.5032619408449293 1103 56-57 0.4983205517984004 1103 58-59 0.5204491607971562 1103 60-61 0.4959043481550154 1103 62-63 0.5347723983677142 1103 64-65 0.5411984663975034 1103 66-67 0.5345878991048423 1103 68-69 0.5086387544465438 1103 70-71 0.48230960876103524 1103 72-73 0.49407827377912383 1103 74-75 0.48942952987133026 1103 76-77 0.4846539927121398 1103 78-79 0.4453033173656529 1103 80-81 0.42081033441699134 1103 82-83 0.39601291304594 1103 84-85 0.41648579096018423 1103 86-87 0.42187487405383806 1103 88-89 0.429954640063837 1103 90-91 0.4326590954949978 1103 92-93 0.36962156398796253 1103 94-95 0.4156569183441654 1103 96-97 0.4469112005228304 1103 98-99 0.42580134847299433 1103 100 0.4312113633074901 1104 1 0.15607013027670646 1104 2 0.16231711864659815 1104 3 0.15231745770612193 1104 4 0.2319090228082672 1104 5 0.23618064506022307 1104 6 0.28863745832913423 1104 7 0.31707962940617307 1104 8 0.29442815003585565 1104 9 0.3648676420268231 1104 10-11 0.3426152291208311 1104 12-13 0.3808691989317836 1104 14-15 0.48686457325026566 1104 16-17 0.46052673072747297 1104 18-19 0.3882827785952898 1104 20-21 0.44201762998257976 1104 22-23 0.458234873385436 1104 24-25 0.49529222617672275 1104 26-27 0.4897040607270853 1104 28-29 0.46235356770315406 1104 30-31 0.42429568693525255 1104 32-33 0.44830075238848366 1104 34-35 0.41213284844565834 1104 36-37 0.4495294731556996 1104 38-39 0.46670423186745325 1104 40-41 0.5230056988325558 1104 42-43 0.4726835802189484 1104 44-45 0.4443088054505395 1104 46-47 0.42729139268038807 1104 48-49 0.39622672709327134 1104 50-51 0.44726072401857664 1104 52-53 0.41909408150496574 1104 54-55 0.4422055118569759 1104 56-57 0.382749884502978 1104 58-59 0.43815228706868936 1104 60-61 0.4270382625064002 1104 62-63 0.4520970506704103 1104 64-65 0.48331552443308823 1104 66-67 0.4828912330502959 1104 68-69 0.4587772954827116 1104 70-71 0.44547211760369265 1104 72-73 0.44537324939239653 1104 74-75 0.4335159330696996 1104 76-77 0.41385371756631173 1104 78-79 0.41625051108769284 1104 80-81 0.4439081000464853 1104 82-83 0.4364333529693276 1104 84-85 0.3467121255848973 1104 86-87 0.36387036728680755 1104 88-89 0.35204439137075383 1104 90-91 0.36099750654242513 1104 92-93 0.3593736055049277 1104 94-95 0.35598998409729177 1104 96-97 0.3816154053117877 1104 98-99 0.39166541370701324 1104 100 0.3583837543997035 1105 1 0.11611632196484223 1105 2 0.15363988495340308 1105 3 0.14711485533373647 1105 4 0.18054678163387194 1105 5 0.1449925328779429 1105 6 0.19110505464644234 1105 7 0.16361138957064725 1105 8 0.2254767099225461 1105 9 0.2563791066663228 1105 10-11 0.28877094596675335 1105 12-13 0.282461120369355 1105 14-15 0.33889253703029 1105 16-17 0.3313506167479616 1105 18-19 0.3198663588075661 1105 20-21 0.3239953206039914 1105 22-23 0.31116952574534196 1105 24-25 0.2807521675692115 1105 26-27 0.30706238401619856 1105 28-29 0.3070045538561743 1105 30-31 0.29677576103267 1105 32-33 0.31352493275233684 1105 34-35 0.33039927762267496 1105 36-37 0.3031419660748753 1105 38-39 0.35652240197916285 1105 40-41 0.34378891615300944 1105 42-43 0.3469665047810082 1105 44-45 0.3165585505570192 1105 46-47 0.30013613965165575 1105 48-49 0.30024022029596154 1105 50-51 0.30535820177131257 1105 52-53 0.26217243858602757 1105 54-55 0.24741535051033026 1105 56-57 0.23338123200935712 1105 58-59 0.2407138803175357 1105 60-61 0.22623014468017288 1105 62-63 0.19655771136614675 1105 64-65 0.23397844694379444 1105 66-67 0.2740510914274523 1105 68-69 0.2538798300810541 1105 70-71 0.3058544545827928 1105 72-73 0.30103206818069594 1105 74-75 0.2805797067865399 1105 76-77 0.2608401206717943 1105 78-79 0.2491873075219999 1105 80-81 0.2686564278225312 1105 82-83 0.23136978576340184 1105 84-85 0.1999198144731018 1105 86-87 0.18357222372388549 1105 88-89 0.21440786014251145 1105 90-91 0.2066660134469558 1105 92-93 0.15939110247423827 1105 94-95 0.16603040568001504 1105 96-97 0.13510039371103844 1105 98-99 0.13912194134787015 1105 100 0.09344167183092011 1106 1 0.08692454275401573 1106 2 0.10822864568813273 1106 3 0.10482772567032583 1106 4 0.12303351886419733 1106 5 0.1522163522892015 1106 6 0.1407074436506477 1106 7 0.15078738211380482 1106 8 0.1255857141483716 1106 9 0.169167522564166 1106 10-11 0.14763383504941885 1106 12-13 0.14647451090151975 1106 14-15 0.22132453904004734 1106 16-17 0.24080800954198622 1106 18-19 0.21258157605902994 1106 20-21 0.16729341055073377 1106 22-23 0.2017797382323181 1106 24-25 0.21877691361181917 1106 26-27 0.218943761378668 1106 28-29 0.253409022603023 1106 30-31 0.24089828249725542 1106 32-33 0.22252752195297631 1106 34-35 0.2533060277380912 1106 36-37 0.21707841686706786 1106 38-39 0.23736259141202254 1106 40-41 0.30207978382355805 1106 42-43 0.2751890130226258 1106 44-45 0.21904329337673545 1106 46-47 0.20984074230138106 1106 48-49 0.1712967413485913 1106 50-51 0.1991232563022649 1106 52-53 0.18683309029005102 1106 54-55 0.22533344614587492 1106 56-57 0.16414565942385195 1106 58-59 0.17411189628711554 1106 60-61 0.1709369581976219 1106 62-63 0.16401024796729757 1106 64-65 0.20204101287589538 1106 66-67 0.18234110829567385 1106 68-69 0.1766265115864698 1106 70-71 0.18968833110298533 1106 72-73 0.145089575351804 1106 74-75 0.14937924622429932 1106 76-77 0.12872846909024815 1106 78-79 0.11904382163825034 1106 80-81 0.11754809636001795 1106 82-83 0.09201049636650538 1106 84-85 0.0707649141928215 1106 86-87 0.09825270013745424 1106 88-89 0.07535759067981118 1106 90-91 0.05833592724098935 1106 92-93 0.019587476992306563 1106 94-95 0.07049265474815058 1106 96-97 0.05154173651509453 1106 98-99 0.06163310899141905 1106 100 0.03501021262898263 1107 1 0.12296277800222555 1107 2 0.11469388274572623 1107 3 0.1502255815206368 1107 4 0.13772049760026306 1107 5 0.1295408632529842 1107 6 0.1892355792589413 1107 7 0.1328232647533909 1107 8 0.17045606915014844 1107 9 0.22984807721027067 1107 10-11 0.23598320390269123 1107 12-13 0.2238010123862395 1107 14-15 0.2691421206702387 1107 16-17 0.2561293128974498 1107 18-19 0.2615111419233145 1107 20-21 0.29949533659461025 1107 22-23 0.302637725281933 1107 24-25 0.31676284399888743 1107 26-27 0.33042427195174895 1107 28-29 0.31430028124295006 1107 30-31 0.2827520028642283 1107 32-33 0.2794889023996987 1107 34-35 0.30357114385482475 1107 36-37 0.2958868638973797 1107 38-39 0.3041412828759178 1107 40-41 0.31951497264359574 1107 42-43 0.3134951694550594 1107 44-45 0.23382539990225126 1107 46-47 0.2116577716707866 1107 48-49 0.26985247861980355 1107 50-51 0.317202359451052 1107 52-53 0.29335898974614594 1107 54-55 0.24881801696077588 1107 56-57 0.27800514114736785 1107 58-59 0.266839451367467 1107 60-61 0.3182851574129444 1107 62-63 0.2856854679490155 1107 64-65 0.31354844624728173 1107 66-67 0.31044966125730866 1107 68-69 0.3131893223369602 1107 70-71 0.28860735764376244 1107 72-73 0.2987280710269289 1107 74-75 0.3099826782088968 1107 76-77 0.3314999056158001 1107 78-79 0.306419039381165 1107 80-81 0.31277662881203483 1107 82-83 0.28815530124505884 1107 84-85 0.29607614930620585 1107 86-87 0.2652309738189018 1107 88-89 0.24180448997431725 1107 90-91 0.2854241651602898 1107 92-93 0.2723546125251026 1107 94-95 0.23614037794257214 1107 96-97 0.25032498700286254 1107 98-99 0.28505038148480466 1107 100 0.24950621568340026 1108 1 0.04527923168124559 1108 2 0.060085268399127756 1108 3 0.08840234398816804 1108 4 0.08785437654351114 1108 5 0.09143319705004416 1108 6 0.15060616920197134 1108 7 0.06610210678068995 1108 8 0.04730802316230154 1108 9 0.1174604192163784 1108 10-11 0.14085854634111428 1108 12-13 0.14264300925589168 1108 14-15 0.13967221868077218 1108 16-17 0.11566886591641179 1108 18-19 0.12163433917686461 1108 20-21 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-0.6731649247852829 2215 30-31 -0.8377943819410589 2215 32-33 -0.7572063617203995 2215 34-35 -0.6905352861925209 2215 36-37 -0.7522608619273896 2215 38-39 -0.7264350452803114 2215 40-41 -0.6883379837858357 2215 42-43 -0.6666882243037904 2215 44-45 -0.6564116296606528 2215 46-47 -0.5792217570672449 2215 48-49 -0.5997843386130839 2215 50-51 -0.6088729139525455 2215 52-53 -0.5884078282709666 2215 54-55 -0.6412152559977216 2215 56-57 -0.6209236378409884 2215 58-59 -0.5994378292836728 2215 60-61 -0.5437489013741441 2215 62-63 -0.5930665551374759 2215 64-65 -0.5511714080208918 2215 66-67 -0.5238446130816072 2215 68-69 -0.5489999246563713 2215 70-71 -0.5174703734529444 2215 72-73 -0.5259137323253071 2215 74-75 -0.5154351428125956 2215 76-77 -0.5399210696755254 2215 78-79 -0.4931144323713106 2215 80-81 -0.5856480780971935 2215 82-83 -0.5268379848881395 2215 84-85 -0.512552843637895 2215 86-87 -0.519952017527558 2215 88-89 -0.47497607376132933 2215 90-91 -0.453732848864945 2215 92-93 -0.40612527777348717 2215 94-95 -0.4532514825929326 2215 96-97 -0.4805964333291719 2215 98-99 -0.46690725865037663 2215 100 -0.4328164779402144 2216 1 -0.671235918596885 2216 2 -0.7429982976176603 2216 3 -0.7046716007525262 2216 4 -0.8320681138186856 2216 5 -0.751018878323066 2216 6 -1.205254946185697 2216 7 -1.0851549913281389 2216 8 -1.362900875842577 2216 9 -1.2733881862768897 2216 10-11 -1.0832992032230564 2216 12-13 -1.0455622626474508 2216 14-15 -1.2106639646846773 2216 16-17 -1.3135912774881362 2216 18-19 -1.2730354262424513 2216 20-21 -1.194274603984475 2216 22-23 -1.260656385941033 2216 24-25 -1.2828905297837991 2216 26-27 -1.314826727508681 2216 28-29 -1.399997315010765 2216 30-31 -1.351967201096528 2216 32-33 -1.43596395229644 2216 34-35 -1.6773954649550262 2216 36-37 -1.4219720673557887 2216 38-39 -1.3602835524411958 2216 40-41 -1.5206057012481367 2216 42-43 -1.4438548185270506 2216 44-45 -1.4002539811905663 2216 46-47 -1.2334105029372893 2216 48-49 -1.399404651077127 2216 50-51 -1.4277546456147974 2216 52-53 -1.618136845286088 2216 54-55 -1.3867596158721298 2216 56-57 -1.379707729312024 2216 58-59 -1.6759033910572683 2216 60-61 -1.8282632204431692 2216 62-63 -1.5961664980452213 2216 64-65 -1.580541805247833 2216 66-67 -1.5111694243137634 2216 68-69 -1.5338301212530006 2216 70-71 -1.4486571737310001 2216 72-73 -1.6080680064018367 2216 74-75 -1.765173975985288 2216 76-77 -1.9121819341605004 2216 78-79 -1.6316662741349681 2216 80-81 -1.5588463793458764 2216 82-83 -1.5809649469115428 2216 84-85 -1.5475706346379532 2216 86-87 -1.5488834397764961 2216 88-89 -1.5213318236377127 2216 90-91 -1.5890345018705148 2216 92-93 -1.6389821337174624 2216 94-95 -1.5604308821582151 2216 96-97 -1.6111469928250166 2216 98-99 -1.5621119861472295 2216 100 -1.5231649660530167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 279299.0 3 47152.0 4 30260.0 5 24431.0 6 21858.0 7 22330.0 8 24002.0 9 27456.0 10 30251.0 11 32614.0 12 35040.0 13 37452.0 14 39553.0 15 41910.0 16 43944.0 17 45776.0 18 44475.0 19 42337.0 20 42520.0 21 45409.0 22 50697.0 23 59722.0 24 71931.0 25 86915.0 26 105926.0 27 124763.0 28 152887.0 29 187212.0 30 225738.0 31 273058.0 32 347375.0 33 457985.0 34 626877.0 35 922347.0 36 1537859.0 37 2575751.0 38 2809823.0 39 801393.0 40 1370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61349128313303 19.030443963829505 13.75866246636944 23.59740228666802 2 16.01724700340103 19.685701627519897 39.018896897344504 25.278154471734567 3 18.62590706047641 24.71529959105666 29.44132678353848 27.21746656492845 4 13.226000870555843 16.5985199904234 37.30890131634605 32.86657782267471 5 14.297954468528411 36.960531747524 34.23466473563277 14.506849048314821 6 31.979657451337957 36.40551357049285 17.627779451685882 13.987049526483315 7 28.567076555742293 31.71532816634018 21.43311738023524 18.28447789768229 8 26.455821893761687 34.93385123397731 19.782898015120896 18.82742885714011 9 25.703481698494983 16.870390202524447 20.382469869714868 37.043658229265695 10-11 24.83466312017339 26.332005553098696 27.929115330225706 20.904215996502202 12-13 25.245065133407056 23.6854243924465 27.93618688845832 23.133323585688125 14-15 22.67423224512694 25.648754897866066 25.063026960210983 26.613985896796017 16-17 22.767625666465875 28.921259559394198 25.535344255354048 22.775770518785883 18-19 22.788182567641368 28.124194269464653 27.09668103861681 21.990942124277172 20-21 23.595453414034147 27.232076573739207 26.86486904257898 22.307600969647666 22-23 23.413142860513492 27.061367916528056 26.775782315145875 22.749706907812577 24-25 23.161247666034985 27.25284514956466 26.664505167095026 22.92140201730533 26-27 22.9647245723939 27.348150154336608 26.694557653892826 22.99256761937666 28-29 22.997207815273015 27.283840816705148 26.83781524392589 22.88113612409595 30-31 23.1069691872387 27.505339724789042 26.58765204499703 22.80003904297523 32-33 22.91732360497009 27.361292304318507 26.79518840980442 22.926195680906982 34-35 23.12066799793087 27.147524047353443 26.84571911618149 22.8860888385342 36-37 23.264417863947827 27.20427404194042 26.653365869436264 22.877942224675497 38-39 23.061692236248113 27.377912086731087 26.66464088125463 22.895754795766166 40-41 23.078853005325065 27.208356235694232 26.807529064030255 22.90526169495045 42-43 23.015827902681394 27.247928728806276 26.76605924245471 22.970184126057624 44-45 22.978223662847473 27.38127161229111 26.678632132548476 22.961872592312947 46-47 23.152919889662808 27.232320519244485 26.646971331060943 22.96778826003176 48-49 23.111598433654592 27.246749015539418 26.590956416595002 23.050696134210988 50-51 23.014261235149547 27.319720200253116 26.58823185296026 23.07778671163708 52-53 23.001536871441054 27.29350519607665 26.614164467504807 23.090793464977484 54-55 23.05441805294747 27.39517244611362 26.631689134812103 22.918720366126806 56-57 22.936013633845622 27.265865040006652 26.646784985202675 23.151336340945047 58-59 22.986654493377745 26.99868908221616 26.758599236880215 23.25605718752588 60-61 23.091728160052792 27.227969307718 26.59438782466708 23.08591470756213 62-63 22.907414537537303 27.339471410027105 26.645643033495737 23.107471018939854 64-65 23.02386150318537 27.086697194903586 26.634139254061008 23.255302047850034 66-67 22.852734320758756 27.096112193695703 26.67736587850208 23.37378760704346 68-69 23.02027507363917 27.20529665512433 26.604648749996567 23.169779521239935 70-71 22.987623914091966 27.00324271899343 26.66352429821055 23.34560906870405 72-73 22.8530455685525 27.104319554433314 26.749382435675074 23.293252441339117 74-75 22.84975411274007 27.116537850493078 26.681206009961546 23.352502026805304 76-77 22.949166123590846 27.0721468501259 26.55675304935508 23.421933976928173 78-79 22.978511311160936 27.097307973027775 26.668313114453547 23.255867601357743 80-81 22.851730289301855 27.038134148141353 26.75478238918638 23.355353173370414 82-83 22.806743670864726 27.016534356985535 26.816765908522637 23.359956063627102 84-85 22.839865686466926 26.961162374272963 26.65931891238585 23.53965302687426 86-87 22.927572799624297 27.097981347613814 26.617078557771606 23.35736729499029 88-89 22.78767484160732 26.996422092342492 26.70976203292092 23.506141033129268 90-91 22.758613500964245 26.932514571528753 26.724959649954442 23.58391227755256 92-93 22.840044284325927 27.0290895857499 26.708471001393946 23.42239512853023 94-95 22.83031287212364 26.772850861106782 26.79710391904044 23.599732347729137 96-97 22.846775729026817 26.857458936990025 26.72186052096051 23.57390481302265 98-99 22.743764812656444 26.901167730283422 26.718690305754517 23.636377151305616 100 22.8739656395892 26.69629290173387 26.65419093158628 23.77555052709065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3882.0 1 2989.5 2 2889.5 3 3614.5 4 3246.5 5 2719.5 6 2351.0 7 2295.5 8 2476.0 9 2549.5 10 2273.0 11 2002.5 12 2040.5 13 2165.0 14 2346.5 15 2552.0 16 2867.5 17 3407.0 18 4259.0 19 5491.0 20 7403.5 21 10260.0 22 14613.0 23 21881.0 24 32019.5 25 44748.0 26 61601.5 27 82367.5 28 107215.5 29 133694.0 30 156689.5 31 178689.0 32 201995.5 33 222967.0 34 243077.0 35 265071.5 36 287806.0 37 311830.5 38 337079.5 39 356437.5 40 374543.0 41 391560.0 42 401708.0 43 408727.0 44 415045.0 45 421916.5 46 427338.5 47 425647.5 48 421563.5 49 423090.5 50 423868.0 51 420267.0 52 408995.0 53 400497.5 54 394005.0 55 377858.5 56 353831.0 57 323524.5 58 295642.0 59 274536.0 60 251114.5 61 221323.0 62 191950.5 63 164435.5 64 137703.5 65 113809.0 66 91317.0 67 71148.5 68 54475.5 69 40867.5 70 30550.0 71 22726.5 72 17244.0 73 13036.5 74 10118.0 75 7705.5 76 5361.0 77 3785.5 78 2827.5 79 2027.0 80 1395.0 81 974.5 82 701.5 83 529.5 84 440.5 85 351.0 86 287.5 87 263.5 88 220.5 89 182.0 90 145.5 91 112.5 92 98.0 93 74.5 94 57.5 95 46.0 96 33.0 97 29.5 98 24.0 99 23.0 100 54.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05259459392206855 2 0.01414641074616621 3 0.01732955514022074 4 0.01371014222515366 5 0.012918395649982733 6 0.011884277674249284 7 0.020036035779835634 8 0.012756814716274382 9 0.013023423256893162 10-11 0.015390583935720518 12-13 0.016638796648617536 14-15 0.013290031797511944 16-17 0.014404940240099574 18-19 0.012175123354924315 20-21 0.015047224451590272 22-23 0.015342109655608012 24-25 0.016404504294740427 26-27 0.009525196042107345 28-29 0.01394443457903077 30-31 0.01286184232318481 32-33 0.014457454043554786 34-35 0.012053937654643052 36-37 0.012098372411412848 38-39 0.018185934088875005 40-41 0.014465533090240203 42-43 0.011484364863321111 44-45 0.01907866874761365 46-47 0.01760424272752494 48-49 0.017281080860108235 50-51 0.012611391875936868 52-53 0.012777012332987928 54-55 0.013395059404422373 56-57 0.01873934878682611 58-59 0.007053007756369561 60-61 0.009949345993091769 62-63 0.015697587709766387 64-65 0.020985323765372203 66-67 0.013803051262035963 68-69 0.014639232593976684 70-71 0.022164864581443174 72-73 0.018311159312498982 74-75 0.018670676890000064 76-77 0.011581313423546123 78-79 0.012595233782566032 80-81 0.0183192383591844 82-83 0.013084016107033795 84-85 0.012772972809645218 86-87 0.016287358117801874 88-89 0.015265358712096546 90-91 0.01565315295299659 92-93 0.01058355115789705 94-95 0.015665271523024715 96-97 0.008713251850222876 98-99 0.006661173992126807 100 0.004887823244677646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2377698E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.93197951996383 #Duplication Level Percentage of deduplicated Percentage of total 1 73.71695465117617 37.545504245678075 2 14.355729949219064 14.62331487535514 3 4.648477359829704 7.102684610695861 4 2.1664841028814728 4.4137329583314555 5 1.2621450957147764 3.214177408308389 6 0.8160274720903433 2.4937136697739937 7 0.5492075389149921 1.9580558986959682 8 0.38936718701716067 1.5864993275923163 9 0.2913859383769503 1.335677638123825 >10 1.6186628927699016 15.04735376106743 >50 0.12305139650606439 4.332304541685285 >100 0.06011141772602904 5.374270514315892 >500 0.0020294423019688594 0.6721874272931925 >1k 3.4966227477265985E-4 0.25659306858463216 >5k 1.5893200589221145E-5 0.043930054498579535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.615809337083519E-5 0.0 3 0.0 8.079046685417595E-6 0.0 1.615809337083519E-5 0.0 4 0.0 8.079046685417595E-6 0.0 1.615809337083519E-5 0.0 5 0.0 1.615809337083519E-5 0.0 2.4237140056252788E-5 0.0 6 0.0 1.615809337083519E-5 0.0 2.4237140056252788E-5 8.079046685417595E-6 7 0.0 1.615809337083519E-5 0.0 4.039523342708798E-5 8.079046685417595E-6 8 0.0 1.615809337083519E-5 0.0 4.039523342708798E-5 8.079046685417595E-6 9 0.0 1.615809337083519E-5 0.0 6.463237348334076E-5 8.079046685417595E-6 10-11 0.0 1.615809337083519E-5 0.0 8.079046685417595E-5 8.079046685417595E-6 12-13 0.0 3.231618674167038E-5 0.0 9.290903688230235E-5 8.079046685417595E-6 14-15 0.0 3.231618674167038E-5 0.0 9.694856022501115E-5 1.615809337083519E-5 16-17 0.0 3.231618674167038E-5 0.0 1.1310665359584633E-4 1.615809337083519E-5 18-19 0.0 4.039523342708798E-5 0.0 1.4138331699480792E-4 1.615809337083519E-5 20-21 0.0 4.039523342708798E-5 0.0 2.0601569047814868E-4 1.615809337083519E-5 22-23 8.079046685417595E-6 4.039523342708798E-5 0.0 3.79715194214627E-4 1.615809337083519E-5 24-25 2.4237140056252788E-5 4.039523342708798E-5 0.0 6.867189682604957E-4 1.615809337083519E-5 26-27 2.4237140056252788E-5 5.251380345521437E-5 0.0 0.0012037779561272217 1.615809337083519E-5 28-29 2.4237140056252788E-5 6.059285014063196E-5 0.0 0.0029165358534357522 1.615809337083519E-5 30-31 4.039523342708798E-5 6.463237348334076E-5 0.0 0.007145916793251862 1.615809337083519E-5 32-33 4.039523342708798E-5 7.271142016875836E-5 0.0 0.01619444908091957 1.615809337083519E-5 34-35 4.8474280112505575E-5 8.482999019688474E-5 0.0 0.030247950790203475 2.4237140056252788E-5 36-37 4.8474280112505575E-5 1.1310665359584633E-4 0.0 0.05439622133291667 2.4237140056252788E-5 38-39 4.8474280112505575E-5 1.535018870229343E-4 0.0 0.08930982158394882 2.4237140056252788E-5 40-41 4.8474280112505575E-5 1.575414103656431E-4 0.0 0.12761662144285635 2.4237140056252788E-5 42-43 4.8474280112505575E-5 1.6965998039376951E-4 0.0 0.1677694834693818 2.4237140056252788E-5 44-45 4.8474280112505575E-5 1.6965998039376951E-4 0.0 0.21613469645163422 2.4237140056252788E-5 46-47 4.8474280112505575E-5 1.777390270791871E-4 0.0 0.2774748584106673 2.4237140056252788E-5 48-49 5.251380345521437E-5 1.777390270791871E-4 0.0 0.34506820250421366 2.4237140056252788E-5 50-51 5.655332679792317E-5 2.2621330719169267E-4 0.0 0.4153033948638915 2.4237140056252788E-5 52-53 5.655332679792317E-5 2.3429235387711027E-4 0.0 0.487223068457479 2.4237140056252788E-5 54-55 5.655332679792317E-5 2.3429235387711027E-4 0.0 0.5676580572574965 2.4237140056252788E-5 56-57 5.655332679792317E-5 2.4237140056252785E-4 0.0 0.663851226617421 2.4237140056252788E-5 58-59 5.655332679792317E-5 2.5852949393336303E-4 0.0 0.7705067614349614 2.4237140056252788E-5 60-61 5.655332679792317E-5 2.7468758730419824E-4 0.0 0.8834720317138129 2.4237140056252788E-5 62-63 5.655332679792317E-5 2.8276663398961585E-4 0.0 0.998691356017896 2.4237140056252788E-5 64-65 5.655332679792317E-5 2.9892472736045106E-4 0.0 1.1208101861913256 2.4237140056252788E-5 66-67 5.655332679792317E-5 3.312409141021214E-4 0.0 1.259693038236997 2.4237140056252788E-5 68-69 5.655332679792317E-5 3.473990074729566E-4 0.0 1.4177232309271077 2.4237140056252788E-5 70-71 5.655332679792317E-5 3.635571008437918E-4 0.0 1.5881830369427337 2.4237140056252788E-5 72-73 5.655332679792317E-5 3.756756708719182E-4 0.0 1.7670814072212782 2.4237140056252788E-5 74-75 6.463237348334076E-5 3.9183376424275335E-4 0.0 1.9491265661837929 2.4237140056252788E-5 76-77 6.463237348334076E-5 4.2011042764171497E-4 0.0 2.1456291791898625 2.4237140056252788E-5 78-79 7.271142016875836E-5 4.2414995098442377E-4 0.0 2.3654559999767324 2.8276663398961583E-5 80-81 8.886951353959355E-5 4.281894743271326E-4 0.0 2.6021680283361253 3.231618674167038E-5 82-83 9.694856022501115E-5 4.281894743271326E-4 0.0 2.848910193155464 3.231618674167038E-5 84-85 9.694856022501115E-5 4.40308044355259E-4 0.0 3.0969005706876995 3.231618674167038E-5 86-87 9.694856022501115E-5 4.4434756769796773E-4 0.0 3.3599947259983236 3.231618674167038E-5 88 9.694856022501115E-5 4.4434756769796773E-4 0.0 3.5741137003019463 3.231618674167038E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18560 0.0 35.64657 1 GGTATCA 11755 0.0 34.3615 1 TCAACGC 19985 0.0 31.514278 4 ATCAACG 20225 0.0 31.164186 3 CAACGCA 21800 0.0 29.041653 5 AACGCAG 23055 0.0 27.480602 6 TATCAAC 23555 0.0 27.2173 2 ACGCAGA 27305 0.0 23.134325 7 GTACATG 40025 0.0 22.850191 1 TACCTGG 9785 0.0 22.624231 2 CGCAGAG 28550 0.0 22.02538 8 TACATGG 40540 0.0 21.796556 2 GTACCTG 11045 0.0 21.627163 1 ACATGGG 42570 0.0 20.116863 3 GCAGAGT 33605 0.0 18.041378 9 TATAACG 1110 0.0 17.784435 2 AGAGTAC 28865 0.0 17.259277 10-11 CATGGGG 32535 0.0 16.627665 4 ACCTGGG 13140 0.0 16.597326 3 GAGTACA 29230 0.0 15.926045 1 >>END_MODULE