FastQCFastQC Report
Wed 25 May 2016
SRR2049430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12377698
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313620.25337506214806665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276670.2235229846454486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157790.12747927764920425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193800.049.928031
GTATCAA327850.039.313281
TCAACGC398550.031.7473054
ATCAACG400300.031.5615463
CAACGCA418700.030.3204945
TATCAAC428450.029.6963272
AACGCAG434150.029.4102926
TATAACG13350.025.7013652
ACGCAGA500000.025.4805417
CGCAGAG515950.024.7019548
ATAACGC16800.023.2211343
GCAGAGT572400.022.142659
GTACATG403250.022.0587581
GTGGTAT95700.022.0206431
TACCTGG100950.021.9295252
TACATGG403550.021.139462
GTACCTG110900.020.6991331
GAGTACT356450.020.2288512-13
CAGAGTA547300.020.11735310-11
AGAGTAC525900.020.00627710-11
Produced by FastQC (version 0.11.5)